FastQCFastQC Report
Fri 27 May 2016
SRR939715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073476
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123830.4028988676013738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79730.25941312051891735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61670.20065229076133992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64750.047.7896841
GTATCAA117100.043.042151
TCAACGC148750.034.044574
TATCAAC149200.033.845912
ATCAACG150450.033.563613
CAACGCA152100.033.482515
AACGCAG158350.032.243546
ACGCAGA176500.028.4170387
CGCAGAG176850.028.1734398
TACATGG108500.026.8141562
GTACATG110850.026.6734161
GCAGAGT180200.025.49129
ACATGGG113600.024.481533
GAGTACT101900.022.58589412-13
AGAGTAC155350.021.81022810-11
CATGGGG59000.021.5587334
AGTACTT107600.021.32327712-13
CAGAGTA169900.020.66862510-11
GTACTTT113900.020.22882314-15
CATGGGA56000.019.1540624
Produced by FastQC (version 0.11.5)