FastQCFastQC Report
Fri 27 May 2016
SRR939715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073476
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132720.4318237721719642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96960.3154734248778907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70240.22853602891319144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58250.051.559051
GTATCAA109800.042.915281
TCAACGC140400.033.1185234
ATCAACG143150.032.5151753
TATCAAC143450.032.4164772
CAACGCA144100.032.2681545
AACGCAG148900.031.1827266
ACGCAGA168200.027.3532287
CGCAGAG170000.026.787168
GTACATG104750.024.8514251
TACATGG102200.024.8226032
GCAGAGT180750.024.206019
ACATGGG106600.023.1342373
GAGTACT108700.021.29259312-13
AGAGTAC157600.021.21634510-11
AGTACTT113750.020.4712412-13
CAGAGTA169300.019.95831310-11
CGCGATA1001.0466771E-519.45932666-67
GTACTTT119500.019.1126214-15
CATGGGA54200.018.9294644
Produced by FastQC (version 0.11.5)