##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939715_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3073476 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.550201465702028 31.0 31.0 34.0 30.0 34.0 2 31.762688890363876 31.0 31.0 34.0 30.0 34.0 3 31.804711343117695 31.0 31.0 34.0 30.0 34.0 4 35.59878359225841 37.0 35.0 37.0 33.0 37.0 5 35.32988251738423 37.0 35.0 37.0 33.0 37.0 6 35.34869932285139 37.0 35.0 37.0 32.0 37.0 7 35.276911549008354 37.0 35.0 37.0 32.0 37.0 8 35.24092005273508 37.0 35.0 37.0 32.0 37.0 9 36.852835681814334 39.0 37.0 39.0 33.0 39.0 10-11 36.73513523450322 39.0 35.0 39.0 32.0 39.0 12-13 36.680040286633115 39.0 35.0 39.0 32.0 39.0 14-15 37.74107915597844 40.0 37.0 41.0 32.0 41.0 16-17 37.70411693470194 39.0 37.0 41.0 32.0 41.0 18-19 37.697914185762315 39.5 37.0 41.0 32.0 41.0 20-21 37.636700432995084 39.0 36.5 41.0 32.0 41.0 22-23 37.498457934924495 39.0 36.0 41.0 32.0 41.0 24-25 37.37611925390014 39.0 36.0 41.0 31.5 41.0 26-27 37.1156490241017 39.0 36.0 40.5 31.0 41.0 28-29 36.91453325160177 39.0 36.0 40.0 31.0 41.0 30-31 36.667746551461605 38.5 35.5 40.0 30.0 41.0 32-33 36.47149595441773 38.0 35.0 40.0 30.0 41.0 34-35 36.450950161966446 38.0 35.0 40.0 30.0 41.0 36-37 36.334916394336574 38.0 35.0 40.0 30.0 41.0 38-39 36.356823999927116 38.5 35.0 40.0 30.0 41.0 40-41 36.26015397549875 38.5 35.0 40.0 30.0 41.0 42-43 36.06434929051016 38.0 35.0 40.0 29.5 41.0 44-45 35.829526568614824 38.0 35.0 40.0 28.5 41.0 46-47 35.506624421339225 38.0 34.5 40.0 27.5 41.0 48-49 35.18709988299892 38.0 34.0 40.0 26.5 41.0 50-51 34.93069931243973 38.0 34.0 40.0 26.0 41.0 52-53 34.683386985940345 38.0 34.0 40.0 26.0 41.0 54-55 34.387015548519 38.0 33.0 40.0 24.0 41.0 56-57 32.35998963388684 36.5 30.5 39.5 15.5 41.0 58-59 27.333645520576702 32.5 17.5 38.0 6.5 40.0 60-61 28.073954050723025 32.5 19.0 38.0 8.0 40.0 62-63 31.152364944447264 34.5 29.0 38.5 8.5 40.0 64-65 31.399306843456724 35.0 30.5 39.0 4.5 40.0 66-67 24.724895362774916 27.0 18.0 35.5 2.0 38.5 68-69 17.23462424954677 13.0 7.0 28.0 2.0 35.0 70-71 16.107030443706083 12.5 7.0 26.5 2.0 33.5 72-73 17.212090968011463 13.0 7.0 28.5 2.0 34.0 74-75 18.20954059833231 17.0 8.5 29.0 2.0 34.5 76-77 19.425184058700964 21.0 8.0 30.0 2.0 34.5 78-79 22.502041662274245 27.5 10.5 33.5 2.0 35.5 80-81 26.15117687595413 31.5 22.5 34.5 2.0 36.0 82-83 27.453785876317237 33.0 26.5 35.0 2.0 36.0 84-85 27.51100447831706 34.0 27.0 35.0 2.0 36.0 86-87 27.271391740166507 34.0 26.5 35.0 2.0 36.0 88-89 26.848276023629275 33.0 26.0 35.0 2.0 35.0 90-91 25.507211216225535 32.5 16.0 35.0 2.0 35.0 92-93 23.046200621055767 31.5 2.0 34.5 2.0 35.0 94-95 15.71297091631755 4.5 2.0 32.0 2.0 34.5 96-97 13.14350478090605 2.0 2.0 30.0 2.0 34.0 98-99 12.806765369243163 2.0 2.0 29.5 2.0 34.0 100-101 12.510071820961024 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34945962377996054 1101 2 0.3606873749348338 1101 3 0.38516419117593514 1101 4 0.2875602348322701 1101 5 0.3343217945252235 1101 6 0.42972484313011705 1101 7 0.41342849224017897 1101 8 0.4146782660691244 1101 9 0.4514420378853288 1101 10-11 0.4667828875358566 1101 12-13 0.3565582209630378 1101 14-15 0.7392639946083079 1101 16-17 0.7925452798094241 1101 18-19 0.6145905356258154 1101 20-21 0.4864065203639214 1101 22-23 0.6364257304387166 1101 24-25 0.5450356920499146 1101 26-27 0.5850524645477719 1101 28-29 0.6887876765979684 1101 30-31 0.4808464967338395 1101 32-33 0.6050032411265676 1101 34-35 0.4424206739123946 1101 36-37 0.449990054055327 1101 38-39 0.5140957550617102 1101 40-41 0.5487713986674052 1101 42-43 0.449520423826975 1101 44-45 0.38185847881349133 1101 46-47 0.4411137561538325 1101 48-49 0.5183772201595787 1101 50-51 0.41696462070942886 1101 52-53 0.31211101052541323 1101 54-55 0.158806706213511 1101 56-57 -2.447250182292702 1101 58-59 -10.17757691852129 1101 60-61 -10.4326061181903 1101 62-63 -4.0815916572877775 1101 64-65 -5.508645546047841 1101 66-67 -10.325099913709185 1101 68-69 -5.385520319102088 1101 70-71 -5.077397667723293 1101 72-73 -5.411895787220287 1101 74-75 -7.0970723109594935 1101 76-77 -8.214998524895877 1101 78-79 -11.320407935483061 1101 80-81 -8.154984988949948 1101 82-83 -4.733389942920894 1101 84-85 -4.064068588786089 1101 86-87 -3.842375454075416 1101 88-89 -3.925370154361186 1101 90-91 -10.126959179330608 1101 92-93 -17.051786872392917 1101 94-95 -11.107455866171023 1101 96-97 -9.181379932185546 1101 98-99 -9.109284980212754 1101 100-101 -8.922906985353531 1102 1 0.3774378802963305 1102 2 0.4157344329730819 1102 3 0.41502393485981415 1102 4 0.2692689255508256 1102 5 0.31644662348093533 1102 6 0.3649918808099173 1102 7 0.4827473187929314 1102 8 0.4839240859135998 1102 9 0.5351723886512758 1102 10-11 0.5642249410685451 1102 12-13 0.4278310199150823 1102 14-15 0.5854951797366255 1102 16-17 0.5119773710552948 1102 18-19 0.5755966097839504 1102 20-21 0.5802973190155924 1102 22-23 0.6211934022339349 1102 24-25 0.5755144283246594 1102 26-27 0.5673811908101598 1102 28-29 0.6219010163837098 1102 30-31 0.7121614727042029 1102 32-33 0.73082769545357 1102 34-35 0.7019908104140811 1102 36-37 0.5006895626158965 1102 38-39 0.55472450969242 1102 40-41 0.46036444676005317 1102 42-43 0.5481477934161418 1102 44-45 0.6543857416042655 1102 46-47 0.7826730334291412 1102 48-49 0.6131232402510207 1102 50-51 0.5811222901684303 1102 52-53 0.5665419962411278 1102 54-55 0.5199354316415352 1102 56-57 -2.290638277610263 1102 58-59 -9.806574035799265 1102 60-61 -10.219564199824315 1102 62-63 -3.9524646923643694 1102 64-65 -5.043677815012899 1102 66-67 -6.489322246252655 1102 68-69 -4.264519000809525 1102 70-71 -4.224614130527419 1102 72-73 -4.305837899158343 1102 74-75 -5.12888654851915 1102 76-77 -7.394376528600446 1102 78-79 -9.942268647027301 1102 80-81 -5.6399137969798545 1102 82-83 -3.0179059501631578 1102 84-85 -2.411195670520229 1102 86-87 -2.345005781479209 1102 88-89 -2.47561128508908 1102 90-91 -8.567420122827585 1102 92-93 -13.306336233271335 1102 94-95 -10.610856414347106 1102 96-97 -9.208954565900463 1102 98-99 -9.253582217638915 1102 100-101 -9.152533420456825 1103 1 0.2679177534569277 1103 2 0.18062216924636232 1103 3 0.1824730750499164 1103 4 0.2291014990882161 1103 5 0.2859050556652747 1103 6 0.23194376169251996 1103 7 0.2748423398036124 1103 8 0.30658441836308725 1103 9 0.3927096806765178 1103 10-11 0.40557635056754293 1103 12-13 0.40553066986623065 1103 14-15 0.5537058602967022 1103 16-17 0.4791069899168434 1103 18-19 0.5277081447268088 1103 20-21 0.3235366857489339 1103 22-23 0.42286194900790264 1103 24-25 0.4834824490562326 1103 26-27 0.42771611157429845 1103 28-29 0.48094142512291427 1103 30-31 0.4506131074284312 1103 32-33 0.45504575495024824 1103 34-35 0.43817686738326955 1103 36-37 0.27859873792717593 1103 38-39 0.22861141833082854 1103 40-41 0.37460732561136467 1103 42-43 0.3688332863531443 1103 44-45 0.4387512441550143 1103 46-47 0.4788668337268547 1103 48-49 0.29151828762197596 1103 50-51 0.28747036586891284 1103 52-53 0.324527364354509 1103 54-55 0.18850934117930507 1103 56-57 -2.5371620173568914 1103 58-59 -10.05960328600472 1103 60-61 -10.323644614145692 1103 62-63 -3.9599526558011284 1103 64-65 -4.979133198322195 1103 66-67 -6.340867907662773 1103 68-69 -4.082314313333754 1103 70-71 -3.7737714257314003 1103 72-73 -4.2241763107196455 1103 74-75 -5.0775208540811665 1103 76-77 -6.579436023336147 1103 78-79 -9.008829291148185 1103 80-81 -4.729925630026326 1103 82-83 -2.497549185265729 1103 84-85 -2.0440073225336874 1103 86-87 -1.886886625317885 1103 88-89 -2.103801652737282 1103 90-91 -4.9194106137487985 1103 92-93 -10.170015855234091 1103 94-95 -8.612319554552716 1103 96-97 -7.963792934820157 1103 98-99 -8.343893995638945 1103 100-101 -8.354039493009584 1104 1 0.18871264035351132 1104 2 0.2199402231950529 1104 3 0.1553354367034494 1104 4 0.1795728370715466 1104 5 0.19546152382423543 1104 6 0.17435786248525886 1104 7 0.23178407984416793 1104 8 0.24436776340579058 1104 9 0.2711950690755671 1104 10-11 0.2909565798077054 1104 12-13 0.35412897235804763 1104 14-15 0.3756511666519913 1104 16-17 0.2578805954013532 1104 18-19 0.20390678043136035 1104 20-21 0.2928181731388335 1104 22-23 0.34794310170337894 1104 24-25 0.33446574767304327 1104 26-27 0.4400059037649555 1104 28-29 0.401565404669185 1104 30-31 0.4562270177433305 1104 32-33 0.5007997928293264 1104 34-35 0.44923979997642505 1104 36-37 0.42322823425158873 1104 38-39 0.3017172174919551 1104 40-41 0.15079895421744993 1104 42-43 0.16632294445200557 1104 44-45 0.27828277280550395 1104 46-47 0.21489045042684296 1104 48-49 0.1999751249243502 1104 50-51 0.1416667946975778 1104 52-53 0.19708501497419206 1104 54-55 0.12572892862274898 1104 56-57 -2.8591503335089428 1104 58-59 -10.374782009202313 1104 60-61 -10.424638253634697 1104 62-63 -4.240813522606988 1104 64-65 -5.324222302685364 1104 66-67 -6.705073683405168 1104 68-69 -4.2322386963489596 1104 70-71 -4.365348337743235 1104 72-73 -4.419201250228117 1104 74-75 -5.118335710693913 1104 76-77 -6.616865761997097 1104 78-79 -9.10157801433343 1104 80-81 -5.0318809500500805 1104 82-83 -2.767704934235038 1104 84-85 -2.220862423557321 1104 86-87 -2.068201008822868 1104 88-89 -2.0993701517036065 1104 90-91 -4.5165766385611334 1104 92-93 -6.7496397479376125 1104 94-95 -7.7876093767884935 1104 96-97 -7.9693102274337075 1104 98-99 -8.355474897889817 1104 100-101 -8.241700630550799 1105 1 0.3423411492516806 1105 2 0.3045082079834103 1105 3 0.29368621293719954 1105 4 0.18909425377818678 1105 5 0.19231797978085297 1105 6 0.27725777392419104 1105 7 0.26321716356773806 1105 8 0.3424000435731429 1105 9 0.36374307961764174 1105 10-11 0.37722160088949863 1105 12-13 0.372949506517692 1105 14-15 0.4202701977838217 1105 16-17 0.46017624633854837 1105 18-19 0.3865362993335637 1105 20-21 0.4929706010575714 1105 22-23 0.48080515105555577 1105 24-25 0.5215239349509702 1105 26-27 0.5010157008142926 1105 28-29 0.5450957176495166 1105 30-31 0.4824384513332305 1105 32-33 0.36220181818609376 1105 34-35 0.35436802191950534 1105 36-37 0.15202550153336603 1105 38-39 0.2898742362822091 1105 40-41 0.35620003197177397 1105 42-43 0.2920487215438996 1105 44-45 0.40891593784581914 1105 46-47 0.372180313591123 1105 48-49 0.24671447799919832 1105 50-51 0.283244983119026 1105 52-53 0.33734108895932025 1105 54-55 0.21289277849128752 1105 56-57 1.2561288131816184 1105 58-59 -8.892776362729542 1105 60-61 -10.183195237914518 1105 62-63 -3.971171649317462 1105 64-65 -5.224672198667349 1105 66-67 -6.609790300784141 1105 68-69 -3.903003972416556 1105 70-71 -3.9238699611231134 1105 72-73 -2.2500192223363484 1105 74-75 -4.408785542122528 1105 76-77 -6.548686032717523 1105 78-79 -9.172733039039437 1105 80-81 -4.751827950711089 1105 82-83 -2.3147400544431456 1105 84-85 -1.8044895686375284 1105 86-87 -1.6896423379582544 1105 88-89 -1.7109679633239736 1105 90-91 -4.288200915012666 1105 92-93 -6.59438710706449 1105 94-95 -7.91510217422095 1105 96-97 -8.086489497359718 1105 98-99 -8.481776173372133 1105 100-101 -8.496214114878214 1106 1 0.19360906219908358 1106 2 0.20714130339261416 1106 3 0.1612480970452772 1106 4 0.11291327696779518 1106 5 0.01047769125485587 1106 6 0.15998839227952288 1106 7 0.1237145776589017 1106 8 0.08495334418964262 1106 9 0.2215770468320244 1106 10-11 0.24061368814725625 1106 12-13 0.2764613463053891 1106 14-15 0.4411772299714656 1106 16-17 0.32035083666916364 1106 18-19 0.3715612593426343 1106 20-21 0.29356791552592654 1106 22-23 0.3472324454122955 1106 24-25 0.34047079333271313 1106 26-27 0.33016686741488854 1106 28-29 0.4339713315426508 1106 30-31 0.44055704652490846 1106 32-33 0.24164476315942807 1106 34-35 0.31372475182946147 1106 36-37 0.33283275402559553 1106 38-39 0.3381241392842327 1106 40-41 0.26572628961168476 1106 42-43 0.054593562367649895 1106 44-45 0.1873400872353841 1106 46-47 0.38579973515128785 1106 48-49 0.3665351931473566 1106 50-51 0.30108476719518507 1106 52-53 0.4824526019407571 1106 54-55 0.46547238170166594 1106 56-57 1.7466308554190277 1106 58-59 -8.50652702572507 1106 60-61 -9.789634878017313 1106 62-63 -3.6070609020619173 1106 64-65 -5.1132710892397455 1106 66-67 -6.577446920089244 1106 68-69 -3.763084732663497 1106 70-71 -3.854859050886823 1106 72-73 -4.305574415846564 1106 74-75 -4.74081732246238 1106 76-77 -6.379993360334161 1106 78-79 -8.965753830699844 1106 80-81 -4.563931548778008 1106 82-83 -2.056970470958202 1106 84-85 -1.5857808757479184 1106 86-87 -1.4914074900082426 1106 88-89 -1.3903981160286634 1106 90-91 -3.9325202124516174 1106 92-93 -6.0913517189428354 1106 94-95 -7.298731147743972 1106 96-97 -7.21062778419738 1106 98-99 -6.743166330928229 1106 100-101 -7.334473256070466 1107 1 0.0810541343899196 1107 2 0.06006032410824602 1107 3 0.05239687293981632 1107 4 0.09816123679202349 1107 5 -0.0403697663722582 1107 6 0.018996552982599724 1107 7 0.21624439561244913 1107 8 0.1818773868763941 1107 9 0.21806166704545404 1107 10-11 0.15181896121693228 1107 12-13 0.16114433437820708 1107 14-15 0.1625519945665701 1107 16-17 0.1892460030219567 1107 18-19 0.21176841565148408 1107 20-21 0.1315465721486504 1107 22-23 0.20033979004506364 1107 24-25 0.31300688875015936 1107 26-27 0.2500978153119391 1107 28-29 0.18271583876173736 1107 30-31 0.16011134658768356 1107 32-33 0.23530452461579898 1107 34-35 0.19244414919293007 1107 36-37 0.08581454938027377 1107 38-39 0.1122296006778285 1107 40-41 0.006686741589085443 1107 42-43 -0.02158346211445661 1107 44-45 0.011508323268024867 1107 46-47 -0.13391150150911102 1107 48-49 -0.1267165331552249 1107 50-51 -0.09549401497681487 1107 52-53 -0.10361142819107272 1107 54-55 -0.22310263399199215 1107 56-57 1.0146785641447025 1107 58-59 -8.877556529805421 1107 60-61 -10.501593446755543 1107 62-63 -4.24262273508138 1107 64-65 -5.474099714475152 1107 66-67 -6.968407372066643 1107 68-69 -4.1103226485454805 1107 70-71 -4.010508768400946 1107 72-73 -2.5168424635552906 1107 74-75 -4.971984930748633 1107 76-77 -6.91737565788595 1107 78-79 -7.76926921048641 1107 80-81 -2.7411129674848524 1107 82-83 -1.5395191213034636 1107 84-85 -1.3110476679638658 1107 86-87 -1.3098004592486703 1107 88-89 -1.1332418071774377 1107 90-91 -0.741434211196129 1107 92-93 -3.5180309405373116 1107 94-95 -6.077764418302666 1107 96-97 -6.540885160217467 1107 98-99 -6.2630658915057555 1107 100-101 -7.03494406586331 1108 1 -0.13221146099752445 1108 2 -0.13624492620972717 1108 3 -0.07419060852738824 1108 4 -0.12569712061971927 1108 5 -0.09994855298268135 1108 6 -0.2164085364138515 1108 7 -0.25796560977347127 1108 8 -0.18450825087283107 1108 9 -0.2694410080650087 1108 10-11 -0.2910894536143971 1108 12-13 -0.1675484421384823 1108 14-15 -0.1662719564379529 1108 16-17 -0.18253110397609618 1108 18-19 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11.459180760521463 2316 96-97 13.141306224862467 2316 98-99 13.054610816964662 2316 100-101 11.85220520344106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 37.0 5 100.0 6 249.0 7 653.0 8 1563.0 9 2867.0 10 4706.0 11 7700.0 12 11304.0 13 15724.0 14 19198.0 15 26245.0 16 36022.0 17 48328.0 18 52245.0 19 51583.0 20 53383.0 21 53708.0 22 53683.0 23 46111.0 24 46548.0 25 54982.0 26 77144.0 27 113673.0 28 161263.0 29 216620.0 30 269040.0 31 295604.0 32 296314.0 33 270466.0 34 239286.0 35 215516.0 36 174768.0 37 111372.0 38 42635.0 39 2820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72288968810502 20.27413380856716 14.667886861630997 23.335089641696822 2 15.230671413873148 21.498181401684082 39.98007318646148 23.291073997981286 3 18.080809822665834 26.297697418189188 31.037041929575853 24.58445082956912 4 12.630682653777026 17.831569206982582 38.342938093546195 31.194810045694194 5 12.803044052150051 38.43347180103921 35.108194118929305 13.655290027881437 6 30.312942089022332 38.14173268312491 17.870970848641736 13.674354379211028 7 26.52521982446579 33.777076436281405 22.275762776661594 17.421940962591204 8 25.267453452687423 35.91646081644234 20.457456029611194 18.358629701259048 9 26.121726670388835 16.11266852254581 20.775499792417445 36.99010501464791 10-11 24.12149956596375 26.959052226209025 28.644033660910317 20.275414546916913 12-13 25.081487540491615 24.529132487125327 28.71273437632179 21.676645596061267 14-15 22.29620149417993 25.593252682604955 27.07933098820108 25.031214835014044 16-17 21.51855098266588 28.733134730838962 27.456502019212124 22.29181226728304 18-19 21.381643324704108 27.629710452727625 29.691467674734472 21.29717854783379 20-21 22.521942582274924 26.76572063682944 29.541925819495578 21.17041096140006 22-23 22.100360066094815 26.745998447679632 29.306220815701895 21.84742067052366 24-25 21.847020667817592 26.863070909335818 29.579817496422617 21.710090926423973 26-27 21.755201376947518 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28.35838737342827 22.204070425136404 52-53 22.19799010962905 27.477212922058985 28.21592147492913 22.108875493382836 54-55 22.26453922128075 27.42782996174864 28.14575730614032 22.161873510830286 56-57 21.226302510244434 27.530288339396265 29.1962758335704 22.0471333167889 58-59 20.825990355699293 27.054603984993054 30.100935570557212 22.01847008875044 60-61 21.937539146375965 27.480387972270876 28.63114015506462 21.95093272628854 62-63 22.000992078502073 27.459994328655856 28.3329192894193 22.20609430342277 64-65 21.63876437590833 27.510241137228387 28.61718465351477 22.23380983334851 66-67 21.603230837216977 27.167537083574118 29.134543874732927 22.09468820447598 68-69 21.76360589584494 26.71106585343513 29.535506539058776 21.989821711661158 70-71 21.71397749404275 26.69787205423032 29.450092878355704 22.138057573371224 72-73 20.92515682287455 26.864974055926545 29.87945773604112 22.33041138515778 74-75 21.3854108204324 27.155510204962436 29.131265565404203 22.327813409200957 76-77 19.597253220697077 27.694546208179283 29.76984436831369 22.938356202809945 78-79 20.509426965690594 27.670015637434858 29.139412280756495 22.681145116118053 80-81 21.573247577203222 27.5917483869046 27.97173589590377 22.863268139988406 82-83 21.920140375627792 27.60796598500456 27.7472060877159 22.72468755165175 84-85 21.989210244703788 27.519927794991396 27.59586799419939 22.894993966105424 86-87 21.66650669521208 27.53494834006569 27.808141625168986 22.99040333955324 88-89 21.844390267550924 27.480997868533652 27.706963885457192 22.96764797845823 90-91 21.23154296852703 27.53029596811572 28.17703615684722 23.061124906510035 92-93 20.181984956049675 27.64627215196696 28.807054656898696 23.36468823508467 94-95 19.46176497928397 27.682543423988104 29.281246680123235 23.574444916604694 96-97 19.46941734697671 27.727509675986767 28.89482952142437 23.908243455612155 98-99 19.717322825550674 27.613621175294167 28.687500721915033 23.981555277240123 100-101 20.17799027471755 27.671841352552658 28.308330344273948 23.841838028455843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 466.0 1 352.5 2 263.0 3 318.0 4 366.5 5 474.0 6 628.5 7 894.5 8 1260.0 9 1605.5 10 1825.5 11 2109.5 12 2517.0 13 2935.5 14 3393.5 15 3733.5 16 4173.5 17 4800.5 18 5395.0 19 5998.0 20 6808.0 21 7957.0 22 9388.0 23 11284.5 24 13779.0 25 17321.5 26 21957.5 27 27243.0 28 32373.5 29 37441.0 30 43246.0 31 49298.5 32 55864.0 33 62870.0 34 69866.5 35 76326.5 36 82079.0 37 87144.5 38 91670.5 39 95464.0 40 98445.5 41 101600.5 42 104767.0 43 107942.5 44 111507.0 45 114373.5 46 116846.5 47 118545.5 48 118265.5 49 117180.0 50 114693.5 51 110603.0 52 106013.0 53 100572.0 54 93685.0 55 85445.5 56 76533.5 57 67789.5 58 59062.0 59 50226.5 60 41178.5 61 32262.0 62 24554.5 63 18001.5 64 12849.0 65 9056.5 66 6150.0 67 4134.5 68 2781.0 69 1841.5 70 1202.0 71 821.0 72 574.5 73 379.5 74 268.5 75 204.5 76 152.5 77 105.5 78 66.5 79 43.5 80 31.5 81 20.0 82 14.0 83 12.5 84 6.5 85 2.5 86 1.0 87 1.0 88 1.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024174582785094142 2 0.007483383634685938 3 4.2297385761268355E-4 4 0.0 5 0.1434532106318709 6 0.0 7 3.253645058559104E-5 8 0.0014316038257660057 9 0.0 10-11 0.0 12-13 0.0 14-15 5.693878852478432E-4 16-17 0.0 18-19 1.7895047822075072E-4 20-21 0.0 22-23 4.0670563231988795E-4 24-25 6.507290117118207E-5 26-27 3.5790095644150144E-4 28-29 0.003579009564415014 30-31 0.01252653347545255 32-33 0.0027655982997752382 34-35 0.0047177853349107 36-37 0.10883442720880203 38-39 0.10771191966359912 40-41 0.12031979426551567 42-43 0.12230451775123671 44-45 0.11786329224630354 46-47 0.1110957105245006 48-49 0.11081915069452307 50-51 0.10994066652871212 52-53 0.0882225857628301 54-55 0.07624917194733259 56-57 0.5550881152154759 58-59 1.5772695150376967 60-61 0.5219172038434658 62-63 0.00424600680141963 64-65 0.11799343804864591 66-67 1.8922711613820964 68-69 2.8505347040289237 70-71 2.7620030219855303 72-73 2.6097649696955503 74-75 1.9973313603229699 76-77 1.4091211384113622 78-79 0.9389694274495718 80-81 0.054156921999716286 82-83 2.602916046847283E-4 84-85 1.626822529279552E-4 86-87 3.5790095644150144E-4 88-89 1.626822529279552E-4 90-91 0.24854594602332997 92-93 0.39626143168191325 94-95 1.9950050041061003 96-97 2.4132122717079945 98-99 1.410162304830101 100-101 0.9448585250055637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3073476.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.03762247212888 #Duplication Level Percentage of deduplicated Percentage of total 1 66.5963707244153 28.661494612513216 2 15.216621709192465 13.097744408428518 3 6.510552336358935 8.405960806117575 4 3.503398038938245 6.031116886776835 5 2.1154825966523294 4.552267067054093 6 1.3130706588318413 3.3906863576420596 7 0.8969383313458572 2.7021465299670977 8 0.6658913866301059 2.292670568418313 9 0.5019670305949406 1.9443120800580564 >10 2.4919526261991654 18.436291635281812 >50 0.12096440576246771 3.5674476665355814 >100 0.06330804327238636 4.895280738876971 >500 0.0028765271442080654 0.8299412378456401 >1k 3.7849041361895976E-4 0.2155403818855965 >5k 1.513961654475839E-4 0.5447151126250263 >10k+ 7.569808272379195E-5 0.43238390997364523 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13272 0.4318237721719642 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9696 0.3154734248778907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7024 0.22853602891319144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 6.507290117118207E-5 0.0 0.0 0.0 4 0.0 6.507290117118207E-5 0.0 0.0 0.0 5 0.0 6.507290117118207E-5 0.0 0.0 0.0 6 0.0 6.507290117118207E-5 0.0 0.0 0.0 7 0.0 6.507290117118207E-5 0.0 3.253645058559104E-5 0.0 8 0.0 6.507290117118207E-5 0.0 3.253645058559104E-5 0.0 9 0.0 6.507290117118207E-5 0.0 3.253645058559104E-5 0.0 10-11 0.0 6.507290117118207E-5 0.0 6.507290117118207E-5 0.0 12-13 0.0 6.507290117118207E-5 0.0 1.1387757704956863E-4 0.0 14-15 0.0 1.4641402763515966E-4 0.0 1.3014580234236415E-4 0.0 16-17 0.0 2.2775515409913726E-4 0.0 2.2775515409913726E-4 0.0 18-19 0.0 2.2775515409913726E-4 0.0 2.602916046847283E-4 0.0 20-21 0.0 2.2775515409913726E-4 0.0 3.741691817342969E-4 0.0 22-23 0.0 2.2775515409913726E-4 0.0 7.483383634685938E-4 0.0 24-25 0.0 2.765598299775238E-4 0.0 0.001252653347545255 0.0 26-27 0.0 3.5790095644150144E-4 0.0 0.0018383094580858936 0.0 28-29 0.0 3.5790095644150144E-4 0.0 0.00406705632319888 0.0 30-31 0.0 3.5790095644150144E-4 0.0 0.010574346440317087 0.0 32-33 0.0 3.5790095644150144E-4 0.0 0.022987002338720065 0.0 34-35 0.0 3.9043740702709247E-4 0.0 0.04146770627133578 0.0 36-37 0.0 4.8804675878386556E-4 0.0 0.0693677126484801 0.0 38-39 0.0 6.344607864190253E-4 0.0 0.11441442848423089 0.0 40-41 0.0 7.320701381757983E-4 0.0 0.16889671498980308 0.0 42-43 0.0 8.78484165810958E-4 0.0 0.22472926419467731 0.0 44-45 0.0 9.11020616396549E-4 0.0 0.28427096876630886 0.0 46-47 0.0 9.435570669821401E-4 0.0 0.3532970486836403 0.0 48-49 0.0 9.435570669821401E-4 0.0 0.42123315750635437 0.0 50-51 0.0 9.598252922749357E-4 0.0 0.4916257683482806 0.0 52-53 1.626822529279552E-5 9.760935175677311E-4 0.0 0.5583905649499133 0.0 54-55 3.253645058559104E-5 9.760935175677311E-4 1.626822529279552E-5 0.63472107802371 0.0 56-57 3.253645058559104E-5 9.760935175677311E-4 3.253645058559104E-5 0.6961661649545986 0.0 58-59 3.253645058559104E-5 9.760935175677311E-4 3.253645058559104E-5 0.7386262329687949 0.0 60-61 3.253645058559104E-5 9.760935175677311E-4 3.253645058559104E-5 0.7740421594312108 0.0 62-63 1.626822529279552E-4 9.760935175677311E-4 3.253645058559104E-5 0.8072130708032208 0.0 64-65 1.9521870351354623E-4 9.923617428605265E-4 3.253645058559104E-5 0.8400260812187894 0.0 66-67 1.9521870351354623E-4 0.001008629968153322 6.507290117118207E-5 0.8675681866394922 0.0 68-69 1.9521870351354623E-4 0.001008629968153322 8.13411264639776E-5 0.9078971171403323 0.0 70-71 2.1148692880634175E-4 0.001008629968153322 9.760935175677312E-5 0.9713594640075276 0.0 72-73 2.2775515409913726E-4 0.001008629968153322 9.760935175677312E-5 1.060346656359119 0.0 74-75 2.2775515409913726E-4 0.001008629968153322 9.760935175677312E-5 1.1760788110920664 3.253645058559104E-5 76-77 2.2775515409913726E-4 0.0010411664187389132 9.760935175677312E-5 1.3614877747540568 3.253645058559104E-5 78-79 2.2775515409913726E-4 0.0010411664187389132 9.760935175677312E-5 1.6353470793329767 3.253645058559104E-5 80-81 2.2775515409913726E-4 0.0010411664187389132 9.760935175677312E-5 1.9723596344985288 3.253645058559104E-5 82-83 2.2775515409913726E-4 0.0010411664187389132 9.760935175677312E-5 2.3177177892392846 3.253645058559104E-5 84-85 2.2775515409913726E-4 0.0010411664187389132 9.760935175677312E-5 2.5828247886106803 3.253645058559104E-5 86-87 2.2775515409913726E-4 0.0010411664187389132 9.760935175677312E-5 2.7840464672572685 3.253645058559104E-5 88-89 2.2775515409913726E-4 0.0010411664187389132 9.760935175677312E-5 2.9774593977633144 3.253645058559104E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5825 0.0 51.55905 1 GTATCAA 10980 0.0 42.91528 1 TCAACGC 14040 0.0 33.118523 4 ATCAACG 14315 0.0 32.515175 3 TATCAAC 14345 0.0 32.416477 2 CAACGCA 14410 0.0 32.268154 5 AACGCAG 14890 0.0 31.182726 6 ACGCAGA 16820 0.0 27.353228 7 CGCAGAG 17000 0.0 26.78716 8 GTACATG 10475 0.0 24.851425 1 TACATGG 10220 0.0 24.822603 2 GCAGAGT 18075 0.0 24.20601 9 ACATGGG 10660 0.0 23.134237 3 GAGTACT 10870 0.0 21.292593 12-13 AGAGTAC 15760 0.0 21.216345 10-11 AGTACTT 11375 0.0 20.47124 12-13 CAGAGTA 16930 0.0 19.958313 10-11 CGCGATA 100 1.0466771E-5 19.459326 66-67 GTACTTT 11950 0.0 19.11262 14-15 CATGGGA 5420 0.0 18.929464 4 >>END_MODULE