FastQCFastQC Report
Fri 27 May 2016
SRR939714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046074
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.17261563573307806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.11408127314044242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.065.3237151
GTATCAA85350.055.5952071
TATCAAC129300.036.8248862
GTACATG92800.034.1965681
TCAACGC143450.033.518994
ATCAACG143550.033.334963
CAACGCA146850.032.6528055
AACGCAG153250.031.412876
ACGCAGA162250.029.3213337
CGCAGAG171850.027.050378
TACATGG105600.026.3469772
ACATGGG108300.025.080323
GCAGAGT172950.025.022359
AGAGTAC147200.022.10595910-11
GAGTACT95550.021.85591712-13
CATGGGG60600.021.20024
AGTACTT103050.020.72321112-13
GTGTAAG11350.020.415121
GTATTAC6452.0008883E-1020.3050291
CAGAGTA164100.019.90125310-11
Produced by FastQC (version 0.11.5)