FastQCFastQC Report
Fri 27 May 2016
SRR939714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046074
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129670.42569550181643645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95060.31207383668289085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71600.235056666384336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.050.970921
GTATCAA110950.041.331631
TCAACGC141250.032.0859154
ATCAACG143250.031.7037873
TATCAAC144450.031.6394212
CAACGCA145550.031.1703995
AACGCAG150750.030.1389586
ACGCAGA169850.026.444537
CGCAGAG171200.026.0983528
GTACATG110050.023.8357241
TACATGG107550.023.5058882
GCAGAGT182850.023.3275579
ACATGGG112950.021.586583
GAGTACT103800.021.13673812-13
AGAGTAC159250.021.04291210-11
AGTACTT107850.020.60521712-13
CAGAGTA172400.019.80691110-11
CATGGGG59700.019.1170374
GTACTTT114300.018.96824814-15
CATGGGA54800.017.7282624
Produced by FastQC (version 0.11.5)