FastQCFastQC Report
Fri 27 May 2016
SRR939711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595653
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153020.9589804299556357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111440.6983974585953212No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81260.5092585919369688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78030.4890160956047462No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54390.34086358374909836No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49720.3115965689282068No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43180.27061021412550224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30430.190705623340413No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28370.17779554828023386No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28100.17610345106360845No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25230.15811708435355304No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23220.14552036062978604No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.1432015607403364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.13524243679546868No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20380.12772200472157794No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18750.11750675115454301No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17860.11192909736640737No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16450.10309258967958572No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA16380.10265389780860876No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG16330.10234054647219665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG140200.028.2310731
TACATGG141350.027.8335152
ACATGGG143400.026.2442043
GTGTAGC7350.025.8274781
CATGGGG35800.024.6584934
TACCTGG12750.024.566492
CATGGGA105750.023.7417054
GAGTACT107500.023.30594412-13
GTATAGG5000.022.7798391
AGTACTT109600.022.772812-13
ATGGGAT37950.021.8858245
GTACTTT118300.021.21937614-15
ATGGGAG36350.021.1517935
GTACCTG15600.020.9910351
ACTTTTT138000.019.2064316-17
TACTTTT130950.019.1151914-15
TATAGGG4001.8189894E-1218.9831962
GTATCAA305750.018.7193281
ATGGGGG19250.018.4912855
ATGGGAC18500.018.214755
Produced by FastQC (version 0.11.5)