FastQCFastQC Report
Fri 27 May 2016
SRR939710_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939710_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607957
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66430.4131329382564335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48030.2987020175290757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35710.2220830532159753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34050.21175939406339844No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23740.14764076402540616No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21400.13308813606334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19730.12270228619297656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG15000.036.3106731
GTACATG115850.031.6910881
TACCTGG12950.029.1829782
TACATGG138550.026.3902662
GTATAGG3908.292773E-925.8623031
ACATGGG138600.025.3590283
GAGTACT100700.023.78945712-13
CATGGGA101750.023.1658614
CATGGGG35350.023.11744
AGTACTT107000.022.05790112-13
GTATCAA274750.021.8796481
GTACTTT110950.021.63558414-15
ATGGGAG35150.021.2159445
CCATGCG1708.346897E-519.4372069
ATGGGAT39200.019.2648035
ACTTTTT132450.019.24837716-17
TATCACG1250.006226467418.8959792
TACTTTT127050.018.57805814-15
CCGGGTC3357.9489837E-1018.318229
ACCTGGG18550.018.081623
Produced by FastQC (version 0.11.5)