FastQCFastQC Report
Fri 27 May 2016
SRR939708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853963
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA134530.7256347618587857No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA107570.5802165415383155No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77630.4187246455296033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64530.3480651987121642No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT47330.25529096319613714No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA44990.2426693520852358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43920.2368979316199946No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT41570.2242223820000723No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG40630.21915216215210334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39930.21537646652063716No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG35260.1901871828078554No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC32070.17298079843017364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32020.1727111058850689No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31020.1673172549829743No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27710.14946360849704118No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG24670.13306630175467363No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22170.11958167449943714No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT20850.11246179130867229No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20340.10971092734860405No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG19510.10523403109986554No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG19350.10437101495553039No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125350.030.7136381
TACATGG147350.024.6565382
ACATGGG147150.023.823173
GCTTATA32150.023.0881
CATGGGG30200.022.9942554
ATGGGAG29900.022.891045
AGTACTT102600.022.77548612-13
TAGGACG2504.110916E-1022.6751524
GAGTACT96750.022.59122712-13
ATGGGCG2303.9035513E-922.5753025
TACACCG1050.002278291222.4775735
CATGGGA109800.022.07114
GTATAGG5507.548806E-1021.5935761
TATCACG1605.2026502E-520.6696912
GTACTTT107400.020.30954714-15
GTATAGC7800.020.3016531
ATGGGAT36500.019.7864235
GTCGGGA5650.019.2324812
CGGGTCG3759.094947E-1218.8811636
TACTTTT123200.018.7587814-15
Produced by FastQC (version 0.11.5)