##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939708_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1853963 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.531052669335903 2.0 2.0 31.0 2.0 33.0 2 22.690942052241603 16.0 16.0 31.0 16.0 34.0 3 27.08024000478974 28.0 25.0 31.0 16.0 34.0 4 32.241468680874426 35.0 32.0 35.0 25.0 37.0 5 32.862190885147115 35.0 33.0 35.0 26.0 37.0 6 33.318862889928226 35.0 33.0 37.0 28.0 37.0 7 33.50753871571331 35.0 33.0 37.0 28.0 37.0 8 33.461891634299064 35.0 33.0 37.0 28.0 37.0 9 34.73405833881259 37.0 34.0 39.0 27.0 39.0 10-11 34.5761296207098 37.0 34.0 39.0 27.0 39.0 12-13 34.600294881828816 37.0 34.0 39.0 27.0 39.0 14-15 35.071573434852795 38.0 33.0 40.0 26.5 41.0 16-17 34.77085707751449 38.0 33.0 40.0 25.0 41.0 18-19 35.24395740368065 38.0 33.5 40.0 27.0 41.0 20-21 35.28074966976148 38.0 34.0 40.0 26.5 41.0 22-23 35.13258058548094 38.0 34.0 40.0 26.0 41.0 24-25 34.898988545078836 38.0 33.0 40.0 25.0 41.0 26-27 34.86561355323704 38.0 33.5 40.0 24.5 41.0 28-29 34.59131978362028 38.0 33.0 40.0 24.0 41.0 30-31 34.47502674001585 38.0 33.0 40.0 24.0 41.0 32-33 34.14329061583214 38.0 33.0 40.0 24.0 41.0 34-35 33.84634024519367 38.0 33.0 40.0 22.0 41.0 36-37 33.45219861453546 37.0 32.0 40.0 19.0 41.0 38-39 33.14578850818489 37.0 31.0 40.0 18.0 41.0 40-41 32.72465011437661 37.0 31.0 40.0 15.5 41.0 42-43 32.484612422146505 37.0 30.5 40.0 15.0 41.0 44-45 32.101037884790586 36.5 30.0 39.5 11.0 41.0 46-47 31.61177704193665 36.0 30.0 39.0 8.5 40.0 48-49 31.147075481010138 36.0 29.5 39.0 7.5 40.0 50-51 30.43958913958909 35.5 28.0 38.5 2.0 40.0 52-53 30.39035002316659 35.5 28.0 38.5 2.0 40.0 54-55 30.536975387318947 36.0 28.0 39.0 2.0 40.0 56-57 30.31982056815589 35.0 28.0 39.0 2.0 40.0 58-59 29.969921460137016 35.0 28.0 39.0 2.0 40.0 60-61 29.302281113485005 34.5 26.0 38.0 2.0 40.0 62-63 28.854236573221797 34.0 26.0 38.0 2.0 40.0 64-65 28.16965117426831 33.5 25.0 37.5 2.0 39.5 66-67 27.564457327357665 33.0 23.0 37.0 2.0 39.0 68-69 27.2830320777707 33.0 23.0 36.0 2.0 39.0 70-71 26.689576598885736 32.5 22.0 36.0 2.0 39.0 72-73 26.05691267840836 32.0 20.5 35.5 2.0 38.0 74-75 25.478679455846745 31.0 19.0 35.0 2.0 37.5 76-77 24.784671269059846 31.0 15.5 34.5 2.0 36.5 78-79 24.095903208424332 30.5 10.0 34.0 2.0 36.0 80-81 23.35348817640913 30.0 6.5 34.0 2.0 36.0 82-83 22.68324475731177 29.0 2.0 34.0 2.0 35.0 84-85 22.007835107820384 29.0 2.0 34.0 2.0 35.0 86-87 21.258926149011604 28.5 2.0 33.5 2.0 35.0 88-89 20.309577105907724 26.5 2.0 33.0 2.0 34.5 90-91 19.22730011332481 25.5 2.0 32.0 2.0 34.0 92-93 18.225609141067 24.5 2.0 32.0 2.0 34.0 94-95 17.280495349691446 20.5 2.0 31.5 2.0 34.0 96-97 16.32276156536026 17.0 2.0 31.0 2.0 34.0 98-99 15.309732448813705 2.0 2.0 31.0 2.0 34.0 100-101 13.81967736141444 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.848689531004055 1101 2 7.911428130891391 1101 3 3.5701708976635658 1101 4 2.293282277706595 1101 5 1.7302892291882657 1101 6 1.4403516136777057 1101 7 1.087268585241027 1101 8 1.0341875587284903 1101 9 0.9110120704180531 1101 10-11 0.9459934084036448 1101 12-13 0.5403732778551671 1101 14-15 0.6982008548771006 1101 16-17 0.8439069779654602 1101 18-19 0.840781866236938 1101 20-21 0.9198342864008779 1101 22-23 0.8404305501106606 1101 24-25 1.0376494533947493 1101 26-27 0.9803850982495987 1101 28-29 1.0933383121189237 1101 30-31 1.1881650745213221 1101 32-33 0.8618255998616959 1101 34-35 1.0507759659647036 1101 36-37 0.9702201956571486 1101 38-39 0.4976373496450677 1101 40-41 0.9509689649893218 1101 42-43 0.6147748979065284 1101 44-45 0.6433974831911797 1101 46-47 0.3834241406428447 1101 48-49 0.40799605742835254 1101 50-51 0.40190680417295965 1101 52-53 0.468231606604089 1101 54-55 0.42457284996115163 1101 56-57 0.28183198852046587 1101 58-59 0.3237250520996362 1101 60-61 0.21477494599952607 1101 62-63 -0.08180474090817214 1101 64-65 -0.02117801422780019 1101 66-67 0.008030517595376097 1101 68-69 -0.14453813288350403 1101 70-71 -0.39641935006397944 1101 72-73 -0.4491119173887377 1101 74-75 -0.4706250023202223 1101 76-77 -0.5949847724031585 1101 78-79 -0.7297087681099406 1101 80-81 -0.9680516809543285 1101 82-83 -0.9153197819880887 1101 84-85 -0.9293502937801712 1101 86-87 -0.9132479020428264 1101 88-89 -0.9132827740078717 1101 90-91 -0.9705296896066038 1101 92-93 -0.8047343723837201 1101 94-95 -1.0623140640235285 1101 96-97 -1.451530114410545 1101 98-99 -1.7186553100360342 1101 100-101 -1.9671192671925581 1102 1 14.675827178003587 1102 2 7.912879841537556 1102 3 3.691126912428974 1102 4 2.2360214504159046 1102 5 1.4426630649618062 1102 6 1.2479489403666904 1102 7 1.1347823002804276 1102 8 0.8658886907186059 1102 9 1.0139397073748455 1102 10-11 0.9836662830488621 1102 12-13 0.6178901397017285 1102 14-15 0.7453513349495822 1102 16-17 0.9962032252750035 1102 18-19 1.0473672977490054 1102 20-21 0.9155919355392541 1102 22-23 0.7663696446414789 1102 24-25 0.786154719994471 1102 26-27 0.8312931242058355 1102 28-29 0.839342727482709 1102 30-31 0.7823234449668988 1102 32-33 0.8319459898199355 1102 34-35 0.9258093085991419 1102 36-37 0.847768832880675 1102 38-39 0.9007006311994914 1102 40-41 0.9096695794771748 1102 42-43 0.7018944384726922 1102 44-45 0.5101277415858476 1102 46-47 0.37069155656101316 1102 48-49 0.2148091407708037 1102 50-51 0.2512601721785366 1102 52-53 0.31596055833624703 1102 54-55 0.25005412092706436 1102 56-57 0.2798566294955833 1102 58-59 0.10945944436636168 1102 60-61 0.36633150924359015 1102 62-63 0.07532664935369127 1102 64-65 0.08238820332228514 1102 66-67 0.08074143050134452 1102 68-69 -0.45845119987730953 1102 70-71 -0.31786717637005424 1102 72-73 -0.038244615542321725 1102 74-75 -0.153687435711781 1102 76-77 -0.38624574474198425 1102 78-79 -0.5054142490858915 1102 80-81 -0.5279239894201027 1102 82-83 -0.44408541000352386 1102 84-85 -0.7095110882506397 1102 86-87 -0.7603425754570985 1102 88-89 -0.5939872531682617 1102 90-91 -0.47249798060759574 1102 92-93 -0.2878799011518076 1102 94-95 -0.17033006357117841 1102 96-97 -0.9851063009473755 1102 98-99 -1.1246985319240448 1102 100-101 -1.0127178550628457 1103 1 14.738077952717173 1103 2 7.629613360664813 1103 3 3.6912487477276628 1103 4 2.1579529813323504 1103 5 1.4938380104449251 1103 6 1.1491681762180477 1103 7 0.6435097099854659 1103 8 0.7584264258180653 1103 9 0.8348309296418677 1103 10-11 0.788100322771875 1103 12-13 0.4890941902839785 1103 14-15 0.5476757699898798 1103 16-17 0.6346906724134982 1103 18-19 0.5617007497785664 1103 20-21 0.600022914665395 1103 22-23 0.8836733470808369 1103 24-25 0.9380344856233762 1103 26-27 0.8570979287543565 1103 28-29 0.9053711321760218 1103 30-31 0.8462223805266191 1103 32-33 0.925017269355429 1103 34-35 1.0247117609023917 1103 36-37 1.0358382436863565 1103 38-39 0.9034255924270269 1103 40-41 0.8556971307348178 1103 42-43 0.8355570312102429 1103 44-45 0.7807585188303108 1103 46-47 0.5651309817573775 1103 48-49 0.38593858930422087 1103 50-51 0.48412642115220805 1103 52-53 0.8775764063422962 1103 54-55 0.5784471898522945 1103 56-57 0.5581452261236279 1103 58-59 0.5102760940133599 1103 60-61 0.4101763252104895 1103 62-63 0.5184213247979912 1103 64-65 0.4324876727207325 1103 66-67 0.4175161205423379 1103 68-69 0.4835372463694867 1103 70-71 0.2162056540640478 1103 72-73 0.017084354478541286 1103 74-75 0.033893309602415655 1103 76-77 0.2854215154058082 1103 78-79 0.040770893482008574 1103 80-81 0.21741301466756013 1103 82-83 -0.1707333501335384 1103 84-85 -0.3658007619733574 1103 86-87 -0.3669880435665611 1103 88-89 -0.5452270891908988 1103 90-91 -0.758239130626869 1103 92-93 -0.5184668883179029 1103 94-95 -0.4582867620111557 1103 96-97 -0.9245847634683049 1103 98-99 -1.1373632229361892 1103 100-101 -1.0756591769464077 1104 1 14.73453570112865 1104 2 8.041156287772115 1104 3 3.8834465058497187 1104 4 2.289386418104499 1104 5 1.4731657285284214 1104 6 1.1001888428706437 1104 7 0.8175810475830119 1104 8 0.8209442813274137 1104 9 1.0524901163454032 1104 10-11 0.841657703166355 1104 12-13 0.5417811365872751 1104 14-15 0.7345390407673591 1104 16-17 0.8351663502191826 1104 18-19 0.7873324653851625 1104 20-21 0.7217689899716717 1104 22-23 0.8228126235720836 1104 24-25 0.8855776379036087 1104 26-27 0.849219691013964 1104 28-29 0.8828496176066309 1104 30-31 0.8318837908128032 1104 32-33 0.8334654760641769 1104 34-35 0.8526493880019501 1104 36-37 0.9153840980416845 1104 38-39 0.7740426366101758 1104 40-41 0.9500875102215645 1104 42-43 0.6814500881438121 1104 44-45 0.5671904427218593 1104 46-47 0.5468223118960722 1104 48-49 0.5340911158148138 1104 50-51 0.5788257097464928 1104 52-53 0.6845905429800432 1104 54-55 0.6044532554479822 1104 56-57 0.7912182032615398 1104 58-59 0.5816917984176051 1104 60-61 0.2568089344721294 1104 62-63 0.3251202193396594 1104 64-65 0.5671424896621815 1104 66-67 0.42637456417521946 1104 68-69 0.5260217481392075 1104 70-71 0.45902721155702864 1104 72-73 0.4048750562440375 1104 74-75 0.11866880515876943 1104 76-77 -0.1260524524929778 1104 78-79 -0.2714616257104687 1104 80-81 -0.5207404266080466 1104 82-83 -0.5971644296266163 1104 84-85 -0.3951042036007948 1104 86-87 -0.5395000586531502 1104 88-89 -0.5164394342965544 1104 90-91 -0.5300552206397455 1104 92-93 -0.34278697163188454 1104 94-95 -0.3158254811994041 1104 96-97 -0.8240865683700385 1104 98-99 -0.9997938239368018 1104 100-101 -1.0568917172488437 1105 1 14.901371957765672 1105 2 8.042080128450696 1105 3 3.90077345010695 1105 4 2.3283263575014246 1105 5 1.6768049265514264 1105 6 1.226182957906893 1105 7 0.9911206286218146 1105 8 1.0803591274361608 1105 9 1.2024676504688756 1105 10-11 0.8254615870232485 1105 12-13 0.8293381916944185 1105 14-15 1.0461778716650656 1105 16-17 1.2041611118343098 1105 18-19 1.0204070301772958 1105 20-21 1.041994991210025 1105 22-23 0.9937147928990342 1105 24-25 1.142699868710352 1105 26-27 1.1305398626313448 1105 28-29 1.1911347314976624 1105 30-31 1.1012123898736164 1105 32-33 0.7894194812916027 1105 34-35 1.0041713141494881 1105 36-37 0.9396323131004038 1105 38-39 1.035716810167294 1105 40-41 1.136973191786943 1105 42-43 1.325023888548202 1105 44-45 1.190163445313658 1105 46-47 1.0022359032034203 1105 48-49 1.1970784472522489 1105 50-51 1.3079587460918987 1105 52-53 1.2410087973266428 1105 54-55 1.25378147094505 1105 56-57 1.0263825055316893 1105 58-59 1.1460262605242448 1105 60-61 1.048147243175194 1105 62-63 0.9401179727520059 1105 64-65 1.1807573587847493 1105 66-67 0.9993942793517441 1105 68-69 0.7597843988934585 1105 70-71 0.9563391146359486 1105 72-73 0.9759348976678304 1105 74-75 0.7860705443463836 1105 76-77 0.7138172180257634 1105 78-79 0.8005249331199913 1105 80-81 0.7777823050233614 1105 82-83 0.8695992238728536 1105 84-85 0.592224083129544 1105 86-87 0.6213026032792435 1105 88-89 0.5810824699330546 1105 90-91 0.8110289770818859 1105 92-93 1.0198444674444929 1105 94-95 1.1725695237047304 1105 96-97 0.46213760272130955 1105 98-99 0.25174892424263895 1105 100-101 0.07813587194342553 1106 1 14.661010019976867 1106 2 7.855077481723292 1106 3 3.5653782753617946 1106 4 2.1446670603254745 1106 5 1.6101574042551476 1106 6 1.1558579694808628 1106 7 0.911272225830082 1106 8 0.8536217212290111 1106 9 1.0309752194104504 1106 10-11 0.9381429489959814 1106 12-13 0.7305275812001071 1106 14-15 0.8523214675456003 1106 16-17 1.169844827909472 1106 18-19 0.8940743854589641 1106 20-21 0.7324370733022576 1106 22-23 0.8532253230306708 1106 24-25 0.8273817154796816 1106 26-27 0.7934031325189252 1106 28-29 1.037909523821284 1106 30-31 0.9091106437323404 1106 32-33 0.7680534904284926 1106 34-35 0.8788814188830258 1106 36-37 0.978393657777687 1106 38-39 1.120328012026114 1106 40-41 1.2062690793141329 1106 42-43 0.9854921103491989 1106 44-45 0.9211897728265228 1106 46-47 0.8899902030435669 1106 48-49 0.8742863463796091 1106 50-51 0.8989394546810949 1106 52-53 0.6944175507442374 1106 54-55 0.7454902479473517 1106 56-57 0.8034863480907184 1106 58-59 0.7816896020394317 1106 60-61 0.6992028768611327 1106 62-63 0.811253248474614 1106 64-65 0.6963647521513323 1106 66-67 0.6809789964663722 1106 68-69 0.792008086666975 1106 70-71 0.7792747982865116 1106 72-73 0.7784512540937847 1106 74-75 0.4342446955155701 1106 76-77 0.7510553841843404 1106 78-79 0.533929384513673 1106 80-81 0.6013490977662634 1106 82-83 0.5243044806403532 1106 84-85 0.5832881024864669 1106 86-87 0.42701217517327095 1106 88-89 0.567573678081132 1106 90-91 0.7824505448529528 1106 92-93 1.128310064355773 1106 94-95 1.270678598573049 1106 96-97 0.487478598170334 1106 98-99 0.04973505294521985 1106 100-101 0.03299464763546389 1107 1 14.608007544510127 1107 2 7.908694773700919 1107 3 3.5921501997671754 1107 4 2.0067321525722477 1107 5 1.2934842393082775 1107 6 1.116049882956176 1107 7 0.8670980510396618 1107 8 0.8253204508782375 1107 9 0.906877058297745 1107 10-11 0.8648418082507945 1107 12-13 0.540804845355737 1107 14-15 0.6393919060229081 1107 16-17 0.957112509891914 1107 18-19 0.8412005705400674 1107 20-21 0.7009869271719538 1107 22-23 0.7760370946742583 1107 24-25 0.7675262095102653 1107 26-27 0.7510501862598815 1107 28-29 0.8310158025640177 1107 30-31 0.7818098745231694 1107 32-33 0.8076031758109181 1107 34-35 0.69869117407967 1107 36-37 0.554972334850234 1107 38-39 0.6571921616082506 1107 40-41 0.9715281122836288 1107 42-43 0.6195360153223817 1107 44-45 0.700724996385361 1107 46-47 0.5532670535413615 1107 48-49 0.45801655182606993 1107 50-51 0.6052538659585132 1107 52-53 0.6856050457490248 1107 54-55 0.4426572047865278 1107 56-57 0.31054958265739074 1107 58-59 0.4056217059917522 1107 60-61 0.4430638921481105 1107 62-63 0.25506072045280703 1107 64-65 0.0017862619268349533 1107 66-67 -0.23195439922165306 1107 68-69 -0.14662312711096348 1107 70-71 0.08359601061718891 1107 72-73 0.28483567939020205 1107 74-75 0.07653408103387349 1107 76-77 0.11647766619314126 1107 78-79 0.1821517358614635 1107 80-81 0.10975780034951654 1107 82-83 -0.04937670149222129 1107 84-85 0.012629025667074245 1107 86-87 0.0209088589982791 1107 88-89 0.23717501129062057 1107 90-91 0.5071416785346408 1107 92-93 0.5648095362129979 1107 94-95 0.6988633318987496 1107 96-97 0.3780524647154593 1107 98-99 -0.01086582016442783 1107 100-101 0.08058946492398356 1108 1 14.383878220813688 1108 2 7.49734526415007 1108 3 3.4311669782906655 1108 4 0.03347040760580455 1108 5 0.20882058417146965 1108 6 0.4070274255478097 1108 7 0.5261571630685751 1108 8 0.4968392944507727 1108 9 0.7343496963978993 1108 10-11 0.6780205640587411 1108 12-13 0.4333100079783492 1108 14-15 0.41678904076736245 1108 16-17 0.5245948279094677 1108 18-19 0.5974112055426417 1108 20-21 0.5294297511265214 1108 22-23 -0.1495686100237208 1108 24-25 0.5382771129692259 1108 26-27 0.5051468563682988 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-0.020084621851239604 2312 12-13 -0.8107653344874066 2312 14-15 -0.12607739758880143 2312 16-17 -0.38849665937037514 2312 18-19 -0.24571990991724135 2312 20-21 -0.171071716583846 2312 22-23 -0.1108802538593423 2312 24-25 0.06291801316491785 2312 26-27 0.11384401879491435 2312 28-29 -0.6481846880366717 2312 30-31 -0.1844758508758204 2312 32-33 -0.04411438124817835 2312 34-35 -0.9039808619839249 2312 36-37 -0.03716519802232909 2312 38-39 0.1676386425888836 2312 40-41 0.08594599880090215 2312 42-43 0.019697233620490806 2312 44-45 0.19035188829458605 2312 46-47 0.19971189076720464 2312 48-49 0.0675343669152717 2312 50-51 -0.12064383430022829 2312 52-53 -0.1128860994944425 2312 54-55 -0.1819865388927937 2312 56-57 -0.16971412026651223 2312 58-59 -0.09390812659177072 2312 60-61 0.008182521335417192 2312 62-63 -0.27663384222456244 2312 64-65 -0.20007569196922503 2312 66-67 -0.12491067756682028 2312 68-69 0.054757924952717474 2312 70-71 -0.15667305193743175 2312 72-73 -0.4140654700257933 2312 74-75 -0.0739525794969147 2312 76-77 -0.1525624092963227 2312 78-79 0.16568248983673328 2312 80-81 0.14661271246874819 2312 82-83 -0.1345526499346974 2312 84-85 -0.03730671938221164 2312 86-87 0.17118790329681488 2312 88-89 0.39493859550451305 2312 90-91 0.5808640052104472 2312 92-93 1.04168444718049 2312 94-95 1.0066176466287935 2312 96-97 0.9271750461884167 2312 98-99 0.7351230443313757 2312 100-101 0.997617607784159 2313 1 -14.205621779186313 2313 2 -7.297625296153825 2313 3 -3.422783164159476 2313 4 -0.9827195259174601 2313 5 -0.3441219609377413 2313 6 -0.728985394965008 2313 7 -0.2542108331327526 2313 8 0.07242523937004819 2313 9 -2.4077210538395164 2313 10-11 -0.45348964486813514 2313 12-13 -0.5901871430188095 2313 14-15 -0.9078636183019668 2313 16-17 -0.6538137665824593 2313 18-19 -0.06789743643265922 2313 20-21 -1.0103316448848716 2313 22-23 -1.9084677078394847 2313 24-25 -1.2284090218835786 2313 26-27 -0.31378215597738546 2313 28-29 0.08579919549058701 2313 30-31 -1.450696164470564 2313 32-33 -0.259745148248804 2313 34-35 -1.0957440286329359 2313 36-37 -1.2644537854707352 2313 38-39 -0.546970441883218 2313 40-41 -0.19360729502179908 2313 42-43 0.020074266531764806 2313 44-45 -0.17987979529326026 2313 46-47 -0.5474295818308867 2313 48-49 0.050582056583966306 2313 50-51 -0.9496946034194877 2313 52-53 -0.037045801622181074 2313 54-55 -0.7152314100372088 2313 56-57 -0.2597385681676734 2313 58-59 -0.22277463045827517 2313 60-61 -1.3698834554748522 2313 62-63 -0.052762246206849994 2313 64-65 0.1161973086645034 2313 66-67 -1.0520178803589566 2313 68-69 -0.22053626757891465 2313 70-71 -0.01529848704688419 2313 72-73 -0.8289252593148078 2313 74-75 -0.1069381969574188 2313 76-77 0.43249231274469935 2313 78-79 0.5243889997989513 2313 80-81 0.3820861431476601 2313 82-83 0.5488103256657233 2313 84-85 0.35305777828822116 2313 86-87 0.30979105559859477 2313 88-89 0.8412289447629 2313 90-91 1.1006743783313233 2313 92-93 1.553512315743518 2313 94-95 1.5907717176550094 2313 96-97 1.4398350354465776 2313 98-99 1.295623468987051 2313 100-101 1.1887607492697647 2314 1 -14.205621779186313 2314 2 -7.204020166759898 2314 3 -3.5225092374050497 2314 4 -1.4658228037567334 2314 5 -1.2550390691747033 2314 6 -6.277514461789696 2314 7 -1.9899947387128414 2314 8 -4.142916122015286 2314 9 -1.452941829841869 2314 10-11 -0.38992466945112625 2314 12-13 -2.978790377192574 2314 14-15 -2.8864527743505946 2314 16-17 -2.47546691980358 2314 18-19 -1.137726011597465 2314 20-21 -1.947372728411274 2314 22-23 -1.255484233519148 2314 24-25 -3.050184369938819 2314 26-27 -4.658281285182014 2314 28-29 -2.02611104011568 2314 30-31 -1.9273065414982042 2314 32-33 -3.761599331975887 2314 34-35 -2.3250608669210457 2314 36-37 -0.612122893290703 2314 38-39 -1.0569090312633023 2314 40-41 -1.9197181477977097 2314 42-43 -1.1367302825452867 2314 44-45 -2.161942530966975 2314 46-47 -2.016863955774973 2314 48-49 -0.8367759104963532 2314 50-51 -1.8338652171278973 2314 52-53 -0.7170705697949167 2314 54-55 -0.12118196696674488 2314 56-57 -0.6134140836149733 2314 58-59 -1.2267491060263964 2314 60-61 -0.08603159996515686 2314 62-63 -2.257681039795198 2314 64-65 -0.7333561600913399 2314 66-67 -1.6930812428955981 2314 68-69 -1.5994455306560482 2314 70-71 -2.0842768422152567 2314 72-73 -2.201269607786582 2314 74-75 -1.6757428045599738 2314 76-77 -0.49972384660193825 2314 78-79 -0.11315574591462862 2314 80-81 0.11887366953557077 2314 82-83 -0.01674268768860543 2314 84-85 0.46069793884926113 2314 86-87 0.6359645032212491 2314 88-89 0.7952661980699318 2314 90-91 1.1267916094718267 2314 92-93 1.487624724017376 2314 94-95 1.5596527284634831 2314 96-97 1.4098061979229541 2314 98-99 1.4243938483191396 2314 100-101 1.6333132144048026 2315 1 -14.205621779186313 2315 2 -7.450244626169436 2315 3 -4.723346850989259 2315 4 -2.9600212471390677 2315 5 -3.5226655865486087 2315 6 -2.718439193431685 2315 7 -3.2217672527635663 2315 8 -3.434248926817709 2315 9 -2.5237211190527447 2315 10-11 -4.937815034415269 2315 12-13 -4.4748361532042935 2315 14-15 -3.3657470822655426 2315 16-17 -4.519026178289863 2315 18-19 -1.6060604205899267 2315 20-21 -3.303995976102847 2315 22-23 -4.245439735440986 2315 24-25 -5.030489935194822 2315 26-27 -4.66719708736084 2315 28-29 -2.486922723082543 2315 30-31 -3.111825820593065 2315 32-33 -4.189935407838213 2315 34-35 -3.406674580622145 2315 36-37 -4.698686246169576 2315 38-39 -3.321110544349498 2315 40-41 -4.442290803004305 2315 42-43 -3.1345085929867125 2315 44-45 -1.6812890123677136 2315 46-47 -0.9291429778504359 2315 48-49 -0.5694561339120199 2315 50-51 -0.33312086560111354 2315 52-53 -0.408022451211135 2315 54-55 -1.9067025389189247 2315 56-57 -3.652430203348576 2315 58-59 -3.3924553709544654 2315 60-61 -4.233102787935959 2315 62-63 -2.2679801602802705 2315 64-65 -3.005852302297839 2315 66-67 -1.753930258237002 2315 68-69 -1.1237058341962651 2315 70-71 -1.7304155244099881 2315 72-73 -1.8052334977832736 2315 74-75 -2.430777910997417 2315 76-77 -2.200594636865855 2315 78-79 -1.7746116074992209 2315 80-81 -1.424340620360649 2315 82-83 -0.8883475596323649 2315 84-85 -0.8170519429659926 2315 86-87 -1.2259223567242579 2315 88-89 -1.874805153603102 2315 90-91 -1.4183230498220425 2315 92-93 -0.5263412805621499 2315 94-95 -0.5455758932991799 2315 96-97 -1.2359798242023299 2315 98-99 -0.9493306575836691 2315 100-101 -0.4806846122356827 2316 1 -14.205621779186313 2316 2 -7.295713837504778 2316 3 -5.088843660007207 2316 4 -2.8033333749001024 2316 5 -2.094284616774157 2316 6 -1.1460612269344566 2316 7 -4.409782553243481 2316 8 -2.448380563705257 2316 9 -1.04164439343662 2316 10-11 -4.352482391024001 2316 12-13 -3.573823561761607 2316 14-15 -1.7172340088780302 2316 16-17 -3.181307654360033 2316 18-19 -2.198617163251683 2316 20-21 -3.260301336343929 2316 22-23 -2.920935631300317 2316 24-25 -3.4549037380946075 2316 26-27 -4.201436476965036 2316 28-29 -3.8638472048650314 2316 30-31 -2.6703704486838546 2316 32-33 -2.983334285667734 2316 34-35 -3.934504611365149 2316 36-37 -2.284980749969332 2316 38-39 -3.177763919485205 2316 40-41 -2.804487050937013 2316 42-43 -6.795452798995296 2316 44-45 -3.5917213422411898 2316 46-47 -4.558326512585975 2316 48-49 -4.047659037756414 2316 50-51 -2.9333613307032387 2316 52-53 -4.54369448522122 2316 54-55 -4.63910140660224 2316 56-57 -4.63540080978736 2316 58-59 -3.46730393881737 2316 60-61 -3.405500032226179 2316 62-63 -2.3575792836480005 2316 64-65 -2.4616097673695236 2316 66-67 -2.867190335956945 2316 68-69 -3.0385284277696556 2316 70-71 -2.988064242436753 2316 72-73 -3.7585619609373246 2316 74-75 -4.359849837457713 2316 76-77 -4.168177506010224 2316 78-79 -4.307287466629287 2316 80-81 -4.0292250043337 2316 82-83 -3.661960319502086 2316 84-85 -3.3572400536408153 2316 86-87 -3.4772581048548226 2316 88-89 -3.355686656053411 2316 90-91 -2.3008208430220556 2316 92-93 -2.4599850878248084 2316 94-95 -1.9364524241081398 2316 96-97 -2.390574533970085 2316 98-99 -2.4631619667487374 2316 100-101 -2.16522179301065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12243.0 3 3796.0 4 3711.0 5 4559.0 6 5400.0 7 7195.0 8 9003.0 9 11654.0 10 13913.0 11 17524.0 12 21124.0 13 23567.0 14 26380.0 15 29858.0 16 33555.0 17 35056.0 18 29965.0 19 28589.0 20 30297.0 21 32892.0 22 36718.0 23 40553.0 24 44764.0 25 50007.0 26 56043.0 27 62463.0 28 70252.0 29 79019.0 30 89710.0 31 101449.0 32 115871.0 33 133228.0 34 151573.0 35 169415.0 36 167794.0 37 93568.0 38 11209.0 39 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.75465429664326 17.98956985541311 13.543028584020295 19.71274726392334 2 14.01983892614551 22.915125804532483 40.64582863201675 22.41920663730526 3 22.201658980202403 28.775800058323526 30.195545810966983 18.826995150507088 4 11.260251245011172 16.13977631682055 40.94823509702035 31.65173734114792 5 11.568075218789575 41.35526674641471 33.32789678478558 13.748761250010125 6 26.60656958871062 38.02969598001758 19.124613026927545 16.239121404344253 7 25.584065069111077 36.72951653036771 21.064104267891466 16.622314132629743 8 23.987244430782994 31.80799875534341 20.92125116078813 23.283505653085466 9 26.921464792140647 19.237550152645532 22.11032177634891 31.730663278864913 10-11 23.618660924577835 26.16935921593916 27.9101054174491 22.301874442033906 12-13 29.83763199827153 24.302265914062712 26.717935560536905 19.14216652712885 14-15 25.124424938952306 25.15718717934936 29.543978243495562 20.17440963820277 16-17 19.738414237594284 28.884382204303517 30.338341640901323 21.03886191720088 18-19 19.077643614284383 30.07202153218281 31.978613669910988 18.87172118362182 20-21 21.846081771237333 27.166623843608573 29.78696271584105 21.200331669313048 22-23 20.401149538657766 27.177320526453038 29.343825283094837 23.077704651794363 24-25 21.807849712285694 26.9440818752556 30.877181981021586 20.370886431437125 26-27 22.8091844856734 24.931492039725022 32.545760939043525 19.713562535558058 28-29 20.13491855427418 26.984305447823115 32.962910911051594 19.917865086851116 30-31 20.017516046239116 28.555885783484907 31.4187321680297 20.00786600224627 32-33 20.032942490379835 28.097284677120076 30.754387894851078 21.11538493764901 34-35 21.738278552756075 26.061773714025037 30.80891626964362 21.39103146357527 36-37 22.411263769298635 25.85257173275975 32.22609684885218 19.510067649089432 38-39 22.806936482408585 25.27258263944731 32.727750252911726 19.192730625232375 40-41 20.664100951861638 26.72364992611163 33.224556608976364 19.387692513050368 42-43 19.371765660128887 28.733245197445196 30.84645307088184 21.048536071544078 44-45 20.45294984620989 28.341942084551864 29.469520048382673 21.73558802085557 46-47 21.165972178674064 27.684041120032177 28.310161509003116 22.83982519229064 48-49 21.957424677944186 27.364540315295464 29.010297028204246 21.667737978556104 50-51 22.379774635139842 28.25381566665054 29.714905314913644 19.65150438329598 52-53 20.98151151616167 28.923658412727942 30.00380726376172 20.09102280734867 54-55 19.15175317357835 27.80936576017719 30.924220204917237 22.114660861327227 56-57 21.42090620891288 29.44805939035361 27.912752577676287 21.21828182305723 58-59 21.712982602140485 27.82449750474868 28.36861630410994 22.0939035890009 60-61 21.069480740748148 26.966573592600408 30.279570787407685 21.684374879243762 62-63 23.386799731384382 26.608484632295216 29.293235561572832 20.711480074747566 64-65 22.1818989479111 27.897202940996067 29.889300587988455 20.031597523104374 66-67 20.527120504529318 27.79964535078223 29.863631376673467 21.809602768014987 68-69 19.99297489063796 29.660146824785667 29.30383868187333 21.043039602703047 70-71 21.175692952344097 28.995351177554856 27.661452876592314 22.167502993508727 72-73 20.876780056555294 27.593653827753396 28.054577859753465 23.474988255937845 74-75 20.753195271055617 29.3127068142621 28.244295447903017 21.689802466779266 76-77 22.292632506173216 26.842657528891035 28.889680771764965 21.975029193170787 78-79 20.972822450372753 27.221617675883557 30.054598419768443 21.750961453975247 80-81 20.900453944504676 29.548651008870706 28.607452909002916 20.943442137621698 82-83 20.828997670434468 28.60061427861193 28.276416183627184 22.293971867326416 84-85 20.943744003459415 27.553384413724142 28.207024229402304 23.295847353414143 86-87 21.815785988252816 27.93645816721222 28.694565102808134 21.55319074172683 88-89 22.699891122305225 26.462658843042046 28.636482569297357 22.200967465355376 90-91 20.922312529847417 26.8874175049168 29.928387821956832 22.26188214327895 92-93 20.7302643811863 28.91507067894161 29.05355297353161 21.30111196634048 94-95 20.620557697314684 29.0069168050271 27.798753769257246 22.57377172840097 96-97 19.85208014326829 28.00630557879837 27.648297120517256 24.493317157416087 98-99 21.230208533943838 28.048053474014946 27.049364790503127 23.67237320153809 100-101 22.235815050858605 27.70236808231935 27.74323672627827 22.31858014054378 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5690.0 1 3949.0 2 2055.5 3 1682.5 4 1394.0 5 1426.0 6 2130.0 7 4601.5 8 6097.0 9 5173.0 10 4134.5 11 3618.5 12 3676.5 13 3840.0 14 3922.5 15 4086.0 16 4326.5 17 4548.5 18 4784.5 19 5255.5 20 5916.0 21 6638.5 22 7542.5 23 8410.5 24 9562.5 25 11509.5 26 14064.5 27 16555.5 28 18852.0 29 21540.0 30 24384.5 31 27639.0 32 31915.0 33 36049.0 34 39535.0 35 42902.0 36 46743.0 37 49731.0 38 50104.0 39 48791.0 40 48030.0 41 48366.0 42 48762.0 43 51242.5 44 55016.5 45 59442.0 46 74388.0 47 123058.5 48 161182.5 49 130527.5 50 82231.0 51 58041.5 52 45935.0 53 40005.0 54 35926.0 55 31704.0 56 27888.5 57 24672.0 58 22146.0 59 19879.0 60 17542.0 61 14709.0 62 11495.0 63 8368.0 64 6064.5 65 4385.0 66 3030.5 67 2199.0 68 1520.0 69 1082.5 70 829.0 71 605.5 72 448.5 73 354.5 74 295.5 75 221.0 76 155.0 77 96.5 78 59.5 79 37.5 80 19.5 81 13.5 82 11.5 83 6.5 84 4.0 85 5.5 86 5.0 87 3.0 88 3.0 89 3.0 90 2.5 91 1.5 92 1.0 93 2.0 94 2.5 95 1.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.26792551954921 2 0.08872884733945607 3 0.11990530555356282 4 0.19299198527694458 5 0.12395069373013377 6 0.1475757606813081 7 0.15561259852542905 8 0.15372475070969593 9 0.14104920108977365 10-11 0.12794214339768378 12-13 0.14094132407173174 14-15 0.14814211502602803 16-17 0.14234372530627634 18-19 0.1499760243327402 20-21 0.17931857324013478 22-23 0.19717221972606785 24-25 0.22246938045689152 26-27 0.21551131279318952 28-29 0.21348861870490402 30-31 0.2281868624131118 32-33 0.21966457798780234 34-35 0.20709690538592193 36-37 0.19447529427502058 38-39 0.18959385920862498 40-41 0.19075353715257531 42-43 0.18401122352495708 44-45 0.20032762250379324 46-47 0.22433025901811418 48-49 0.22686536894209863 50-51 0.23649339280233747 52-53 0.26246478489592295 54-55 0.24978923527600064 56-57 0.22842958570370606 58-59 0.1859799791042216 60-61 0.1965788961268375 62-63 0.20148730044774357 64-65 0.1888656893368422 66-67 0.19870946723316482 68-69 0.18651936419443108 70-71 0.20008489921319897 72-73 0.2207163789137108 74-75 0.24965438900344827 76-77 0.25046346663876246 78-79 0.2454471852998145 80-81 0.24908803465872836 82-83 0.2414557356322645 84-85 0.21240984852448513 86-87 0.2242763205090932 88-89 0.225516906216575 90-91 0.21189743268878614 92-93 0.2107377547448358 94-95 0.2495465119854064 96-97 0.276380920223327 98-99 0.293371550564925 100-101 0.28897556207971786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1853963.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.32344346125439 #Duplication Level Percentage of deduplicated Percentage of total 1 83.97104845913374 54.85278036402475 2 10.038812675990306 13.115396245163529 3 2.947404668631405 5.776038666863424 4 1.14825965559724 3.0003309876498294 5 0.5851855397687877 1.9113167260715016 6 0.33620306271607253 1.3177165055300355 7 0.20217450012155158 0.9244714169598279 8 0.14236195076913905 0.7439658273681387 9 0.10168582443694514 0.5978221383076041 >10 0.47255162046965105 5.472514573946433 >50 0.03111043542894811 1.3974938838114235 >100 0.017007658218475826 1.9903772406855262 >500 0.0026245545414180687 1.2443473759520154 >1k 0.0031494654497016827 5.020250738857387 >5k 2.099643633134455E-4 0.974904507937936 >10k+ 2.099643633134455E-4 1.6602728008706693 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13453 0.7256347618587857 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10757 0.5802165415383155 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7763 0.4187246455296033 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6453 0.3480651987121642 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4733 0.25529096319613714 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4499 0.2426693520852358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4392 0.2368979316199946 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4157 0.2242223820000723 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4063 0.21915216215210334 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3993 0.21537646652063716 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3526 0.1901871828078554 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3207 0.17298079843017364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3202 0.1727111058850689 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3102 0.1673172549829743 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2771 0.14946360849704118 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2467 0.13306630175467363 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2217 0.11958167449943714 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2085 0.11246179130867229 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2034 0.10971092734860405 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1951 0.10523403109986554 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1935 0.10437101495553039 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6181552706283784E-4 0.0 12-13 0.0 0.0 0.0 4.584773266780405E-4 0.0 14-15 0.0 0.0 0.0 5.933235992304054E-4 0.0 16-17 0.0 0.0 0.0 7.821083808037161E-4 0.0 18-19 0.0 0.0 0.0 9.978624168875E-4 0.0 20-21 0.0 0.0 0.0 0.0016181552706283782 0.0 22-23 0.0 0.0 0.0 0.0034790338318510133 0.0 24-25 0.0 0.0 0.0 0.006041113010345945 0.0 26-27 0.0 0.0 0.0 0.007389575735869594 0.0 28-29 0.0 0.0 0.0 0.01181253347558716 0.0 30-31 0.0 0.0 0.0 0.02303174335194392 0.0 32-33 0.0 0.0 0.0 0.044418362178748984 0.0 34-35 0.0 0.0 0.0 0.08193259520281689 0.0 36-37 0.0 0.0 0.0 0.1352777806245324 0.0 38-39 0.0 0.0 0.0 0.20067822281242936 0.0 40-41 0.0 0.0 0.0 0.2742233798624892 0.0 42-43 0.0 0.0 0.0 0.3485236760388422 0.0 44-45 0.0 0.0 0.0 0.4312114103679523 0.0 46-47 0.0 0.0 0.0 0.533317007944603 5.393850902094594E-5 48-49 0.0 0.0 0.0 0.652035666299705 5.393850902094594E-5 50-51 0.0 0.0 0.0 0.7764448373565168 5.393850902094594E-5 52-53 0.0 0.0 0.0 0.8897696448095243 5.393850902094594E-5 54-55 0.0 0.0 0.0 1.0118594599784354 5.393850902094594E-5 56-57 0.0 0.0 0.0 1.1509399054889444 5.393850902094594E-5 58-59 0.0 0.0 0.0 1.3307708945647783 5.393850902094594E-5 60-61 0.0 0.0 0.0 1.5772698807905012 5.393850902094594E-5 62-63 0.0 0.0 0.0 1.800089861556029 5.393850902094594E-5 64-65 0.0 0.0 0.0 1.9876340574218578 5.393850902094594E-5 66-67 0.0 0.0 0.0 2.196214271805856 5.393850902094594E-5 68-69 0.0 0.0 0.0 2.443684151193956 5.393850902094594E-5 70-71 0.0 0.0 0.0 2.721440503397317 5.393850902094594E-5 72-73 0.0 0.0 0.0 3.027865173145311 5.393850902094594E-5 74-75 0.0 0.0 0.0 3.3326716876226765 5.393850902094594E-5 76-77 0.0 0.0 0.0 3.614041919930441 5.393850902094594E-5 78-79 5.393850902094594E-5 0.0 0.0 3.953719680489848 5.393850902094594E-5 80-81 5.393850902094594E-5 0.0 0.0 4.335739170630697 5.393850902094594E-5 82-83 5.393850902094594E-5 0.0 0.0 4.721534356403014 5.393850902094594E-5 84-85 5.393850902094594E-5 0.0 0.0 5.192471478664892 5.393850902094594E-5 86-87 5.393850902094594E-5 0.0 0.0 5.685172789316724 5.393850902094594E-5 88-89 5.393850902094594E-5 0.0 0.0 6.123558021384461 5.393850902094594E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12535 0.0 30.713638 1 TACATGG 14735 0.0 24.656538 2 ACATGGG 14715 0.0 23.82317 3 GCTTATA 3215 0.0 23.088 1 CATGGGG 3020 0.0 22.994255 4 ATGGGAG 2990 0.0 22.89104 5 AGTACTT 10260 0.0 22.775486 12-13 TAGGACG 250 4.110916E-10 22.675152 4 GAGTACT 9675 0.0 22.591227 12-13 ATGGGCG 230 3.9035513E-9 22.575302 5 TACACCG 105 0.0022782912 22.477573 5 CATGGGA 10980 0.0 22.0711 4 GTATAGG 550 7.548806E-10 21.593576 1 TATCACG 160 5.2026502E-5 20.669691 2 GTACTTT 10740 0.0 20.309547 14-15 GTATAGC 780 0.0 20.301653 1 ATGGGAT 3650 0.0 19.786423 5 GTCGGGA 565 0.0 19.232481 2 CGGGTCG 375 9.094947E-12 18.881163 6 TACTTTT 12320 0.0 18.75878 14-15 >>END_MODULE