##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939707_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1044852 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41284985816173 31.0 31.0 34.0 30.0 34.0 2 31.573975070153477 31.0 31.0 34.0 30.0 34.0 3 31.616191575457577 31.0 31.0 34.0 30.0 34.0 4 35.297578987263265 37.0 35.0 37.0 33.0 37.0 5 35.10721901283627 37.0 35.0 37.0 32.0 37.0 6 35.194521329336595 37.0 35.0 37.0 32.0 37.0 7 35.16435342038872 37.0 35.0 37.0 32.0 37.0 8 35.184918055380095 37.0 35.0 37.0 32.0 37.0 9 36.677776374070206 39.0 35.0 39.0 32.0 39.0 10-11 36.5745837688017 38.5 35.0 39.0 32.0 39.0 12-13 36.536219005179674 38.0 35.0 39.0 32.0 39.0 14-15 37.196035897907066 39.0 36.0 40.5 32.0 41.0 16-17 37.18713176603002 39.0 36.0 40.0 31.5 41.0 18-19 37.405577536340076 39.0 36.0 40.5 32.0 41.0 20-21 37.36203213469467 39.0 36.0 41.0 32.0 41.0 22-23 37.24472556878869 39.0 36.0 40.5 31.5 41.0 24-25 37.06115507268015 39.0 36.0 40.0 31.0 41.0 26-27 36.45960863356724 38.5 34.5 40.0 30.0 41.0 28-29 36.18870040924456 38.0 35.0 40.0 30.0 41.0 30-31 36.09436695340584 38.0 35.0 40.0 30.0 41.0 32-33 35.959309548146535 38.0 35.0 40.0 30.0 41.0 34-35 35.96997230229736 38.0 35.0 40.0 30.0 41.0 36-37 35.69529129484367 38.0 34.5 40.0 29.5 41.0 38-39 35.49710915995758 38.0 34.5 40.0 28.0 41.0 40-41 35.321368480894904 38.0 34.0 40.0 27.5 41.0 42-43 35.34039031365208 38.0 34.0 40.0 27.5 41.0 44-45 35.21634547285166 38.0 34.0 40.0 27.0 41.0 46-47 34.82541211578291 38.0 33.0 40.0 25.0 41.0 48-49 34.527533086025585 38.0 33.0 40.0 24.0 41.0 50-51 34.31480391481281 38.0 33.0 40.0 24.0 41.0 52-53 33.7480600123271 37.0 33.0 40.0 23.0 41.0 54-55 33.56545089639489 37.0 33.0 40.0 23.0 41.0 56-57 31.80613522297895 36.0 30.0 39.5 14.5 40.5 58-59 26.982332904564473 32.5 17.0 38.0 4.0 40.0 60-61 27.636940447068103 32.0 18.5 38.0 5.5 40.0 62-63 30.549857778900744 34.5 29.0 38.0 2.0 40.0 64-65 30.44743896743271 35.0 29.5 38.0 2.0 40.0 66-67 23.897415136306385 26.5 16.5 35.0 2.0 38.0 68-69 16.81433112057976 13.0 7.0 28.0 2.0 34.5 70-71 15.76256733010991 12.5 7.0 26.0 2.0 33.0 72-73 16.80805511211157 13.0 7.0 28.0 2.0 34.0 74-75 17.727577685643517 17.0 7.5 29.0 2.0 34.0 76-77 18.669734565278148 20.5 7.0 29.5 2.0 33.5 78-79 21.53096754372868 26.5 9.0 33.0 2.0 35.0 80-81 25.121919180898345 30.5 20.5 34.0 2.0 36.0 82-83 26.403814607236242 32.5 25.0 35.0 2.0 36.0 84-85 26.46583583129477 33.0 25.0 35.0 2.0 35.5 86-87 26.16099122172327 33.0 25.0 35.0 2.0 35.0 88-89 25.487390080126183 32.5 21.5 35.0 2.0 35.0 90-91 24.04150444273447 31.5 9.5 34.0 2.0 35.0 92-93 21.868931676447957 30.5 2.0 34.0 2.0 35.0 94-95 15.161937767262732 4.5 2.0 31.5 2.0 34.0 96-97 12.679575671961196 2.0 2.0 29.0 2.0 34.0 98-99 12.301895388054959 2.0 2.0 29.0 2.0 34.0 100-101 11.988011699264586 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.512535979391501 1101 2 0.5936851128905296 1101 3 0.5666003242786388 1101 4 0.37770713159064684 1101 5 0.6730791961548803 1101 6 0.6296244398787394 1101 7 0.44313513569211693 1101 8 0.6069220640557731 1101 9 0.7724723667270936 1101 10-11 0.7785219246808026 1101 12-13 0.6778853157335689 1101 14-15 1.0412738081838384 1101 16-17 0.9906288918937278 1101 18-19 0.836452824466356 1101 20-21 0.9558542862118529 1101 22-23 0.9576612104927591 1101 24-25 0.8287840756285831 1101 26-27 0.9399248699924811 1101 28-29 1.2392979049043475 1101 30-31 1.2036313457601011 1101 32-33 1.2912998983166162 1101 34-35 1.0807550432592308 1101 36-37 0.9179767529093823 1101 38-39 1.0493690015450525 1101 40-41 1.3615994936388844 1101 42-43 0.9278609980949923 1101 44-45 0.7986527499680207 1101 46-47 0.7283601536071629 1101 48-49 1.142228744179441 1101 50-51 0.9928116913040839 1101 52-53 1.0893744097653268 1101 54-55 1.2935082145108439 1101 56-57 -1.5104826177124515 1101 58-59 -9.402805053355674 1101 60-61 -9.239075832049021 1101 62-63 -2.179407139247516 1101 64-65 -2.969868940891576 1101 66-67 -8.793507971268554 1101 68-69 -4.061131360600315 1101 70-71 -3.8607869604186593 1101 72-73 -3.380224149008985 1101 74-75 -5.6423380765933135 1101 76-77 -6.545004385110168 1101 78-79 -9.259684998128623 1101 80-81 -5.792720398602686 1101 82-83 -2.1929968959063686 1101 84-85 -1.3453827122648683 1101 86-87 -0.971246138746519 1101 88-89 -1.1418880421383513 1101 90-91 -7.858661625252157 1101 92-93 -15.571041289645136 1101 94-95 -10.746982168328204 1101 96-97 -8.697134836341302 1101 98-99 -8.312963255793345 1101 100-101 -8.294614940480045 1102 1 0.24773525351213976 1102 2 0.23645879454151242 1102 3 0.3549431717232103 1102 4 0.18183230661438898 1102 5 0.28485613186037995 1102 6 0.1859829015628165 1102 7 0.4247613143485225 1102 8 0.3621662486980739 1102 9 0.3837531978328528 1102 10-11 0.3331268850740301 1102 12-13 0.28803032841361187 1102 14-15 0.4626926347006233 1102 16-17 0.38202441454971137 1102 18-19 0.5264176402059206 1102 20-21 0.5198615711162375 1102 22-23 0.4711539500672117 1102 24-25 0.4344421159501408 1102 26-27 0.42390917742066847 1102 28-29 0.573026163609839 1102 30-31 0.41441541949691185 1102 32-33 0.5048533454276196 1102 34-35 0.27599178633253985 1102 36-37 -8.375244587810471E-4 1102 38-39 0.07652012088104954 1102 40-41 0.35110786050823606 1102 42-43 0.3525420387396636 1102 44-45 0.2891892283434103 1102 46-47 0.39374494473734245 1102 48-49 0.32332895404474016 1102 50-51 0.40893655952630326 1102 52-53 0.43912244085019836 1102 54-55 0.3786138162498176 1102 56-57 -2.791205200589662 1102 58-59 -10.28578148512334 1102 60-61 -10.650487706573724 1102 62-63 -4.38671032117103 1102 64-65 -5.06292037584149 1102 66-67 -6.304636870340641 1102 68-69 -4.311831455578236 1102 70-71 -4.220249013312548 1102 72-73 -4.546824722793122 1102 74-75 -5.31196668355118 1102 76-77 -7.36398903524219 1102 78-79 -9.660440415297106 1102 80-81 -5.699041489249481 1102 82-83 -3.707556913139477 1102 84-85 -3.2681495072006825 1102 86-87 -3.118949859857402 1102 88-89 -3.1838415192767435 1102 90-91 -8.815777951989258 1102 92-93 -12.480599299612921 1102 94-95 -9.539483155641635 1102 96-97 -8.46020490785217 1102 98-99 -8.696307686203252 1102 100-101 -8.61545508709201 1103 1 0.30923866470921624 1103 2 0.31126413518916607 1103 3 0.24837866705486533 1103 4 0.3835451101562697 1103 5 0.28208096279121975 1103 6 0.22083027968643165 1103 7 0.32638318576155 1103 8 0.3116864947614104 1103 9 0.41400676431020145 1103 10-11 0.4221621100937796 1103 12-13 0.4637305803403251 1103 14-15 0.769408444468624 1103 16-17 0.7127738841123019 1103 18-19 0.5045355438973758 1103 20-21 0.35738374513946525 1103 22-23 0.5607891780683545 1103 24-25 0.6572519187317667 1103 26-27 0.48551445247793623 1103 28-29 0.6961746016145014 1103 30-31 0.7644941776522316 1103 32-33 0.6194278277852945 1103 34-35 0.7614823974660112 1103 36-37 0.7835576775049518 1103 38-39 0.5945363594710571 1103 40-41 0.5874568610001631 1103 42-43 0.5322708821128046 1103 44-45 0.5273643248656299 1103 46-47 0.5094809235496527 1103 48-49 0.5113932425629457 1103 50-51 0.5342079894464504 1103 52-53 0.6709186236883724 1103 54-55 0.5725414879401214 1103 56-57 -2.66256729431435 1103 58-59 -10.062610189862244 1103 60-61 -10.160772229726632 1103 62-63 -3.835047952662368 1103 64-65 -4.788331433784638 1103 66-67 -6.261018020060643 1103 68-69 -4.348401823629754 1103 70-71 -4.248408159444951 1103 72-73 -4.28538367215436 1103 74-75 -5.201499053898363 1103 76-77 -6.429764517356386 1103 78-79 -9.014252111255844 1103 80-81 -4.774907704733785 1103 82-83 -2.726235266820737 1103 84-85 -2.1027163870361107 1103 86-87 -1.9860796344152902 1103 88-89 -2.0328618870798145 1103 90-91 -4.826289805464306 1103 92-93 -9.236003053171945 1103 94-95 -7.917241554441132 1103 96-97 -7.62389640445588 1103 98-99 -7.575165469347516 1103 100-101 -7.881609204627999 1104 1 0.18098166811448735 1104 2 0.26486673311894293 1104 3 0.1811346826604847 1104 4 0.14868729719819385 1104 5 0.1665452525325506 1104 6 0.22760248725019494 1104 7 0.23061384223495196 1104 8 0.20988463200967544 1104 9 0.2699186331508301 1104 10-11 0.3353795288521013 1104 12-13 0.19685982283632342 1104 14-15 0.35865815693733083 1104 16-17 0.3864427833748678 1104 18-19 0.25152266700586523 1104 20-21 0.33729601990661706 1104 22-23 0.4123316988720873 1104 24-25 0.5338626577435619 1104 26-27 0.5156229250666158 1104 28-29 0.35465733846135095 1104 30-31 0.48366067518283984 1104 32-33 0.3863324124999039 1104 34-35 0.527396972483487 1104 36-37 0.44572194243966834 1104 38-39 0.5854582637174985 1104 40-41 0.3166373404445011 1104 42-43 0.4381714752165635 1104 44-45 0.5529151446116956 1104 46-47 0.4509519561816333 1104 48-49 0.5126741662713101 1104 50-51 0.6712138302781483 1104 52-53 0.20016111968548955 1104 54-55 0.23035219086955294 1104 56-57 -2.7355844559858653 1104 58-59 -10.433615760076261 1104 60-61 -10.506144667315432 1104 62-63 -4.375816506887386 1104 64-65 -5.432288766396322 1104 66-67 -6.533631510352233 1104 68-69 -4.067691226692244 1104 70-71 -4.200475002013112 1104 72-73 -4.182780104511217 1104 74-75 -4.970781691518731 1104 76-77 -6.764735087683157 1104 78-79 -8.811490683296526 1104 80-81 -4.898831435649598 1104 82-83 -3.1478147720414036 1104 84-85 -2.764969730292094 1104 86-87 -2.576859464377179 1104 88-89 -2.4049048250858114 1104 90-91 -4.051718847252186 1104 92-93 -5.666366357413876 1104 94-95 -7.1476680028095325 1104 96-97 -7.302708167304582 1104 98-99 -7.748936977815594 1104 100-101 -7.662231850817927 1105 1 0.20933050708279666 1105 2 0.27870834873046846 1105 3 0.39181466148205857 1105 4 0.2701228920851193 1105 5 0.2394217369134637 1105 6 0.19869393267792645 1105 7 0.4001925427871029 1105 8 0.23417931471144016 1105 9 0.4242261741840352 1105 10-11 0.40127847749441514 1105 12-13 0.33492048390583307 1105 14-15 0.4138317444118016 1105 16-17 0.42456395423939597 1105 18-19 0.6216762106107367 1105 20-21 0.4517124785708688 1105 22-23 0.47651368208061484 1105 24-25 0.5122682246446999 1105 26-27 0.5953672711826457 1105 28-29 0.5689830324330671 1105 30-31 0.5302229624530881 1105 32-33 0.5594131174390142 1105 34-35 0.7160589496410381 1105 36-37 0.5397087815592485 1105 38-39 0.626283466047127 1105 40-41 0.48873097935229737 1105 42-43 0.7072018057513176 1105 44-45 0.5887142520922239 1105 46-47 0.6448215575733585 1105 48-49 0.40759890721374603 1105 50-51 0.49958536041958723 1105 52-53 0.5177543425884465 1105 54-55 0.34071071140505893 1105 56-57 1.3599189098714852 1105 58-59 -8.504953026546271 1105 60-61 -9.88955741975473 1105 62-63 -3.7385235638422287 1105 64-65 -4.993278857917385 1105 66-67 -5.931634687116471 1105 68-69 -3.756916701315948 1105 70-71 -3.616404205552934 1105 72-73 -1.7950598110387084 1105 74-75 -4.169401311819765 1105 76-77 -6.009493426689286 1105 78-79 -8.643163779128916 1105 80-81 -4.218406354339564 1105 82-83 -2.010514265271869 1105 84-85 -1.5485904851517844 1105 86-87 -1.5432803100015633 1105 88-89 -1.677202684551812 1105 90-91 -3.9813913379866257 1105 92-93 -6.095116073774214 1105 94-95 -7.4844125758372915 1105 96-97 -7.335602804623998 1105 98-99 -7.847579289289763 1105 100-101 -7.635838234601302 1106 1 0.18431345631782747 1106 2 0.3572378570790278 1106 3 0.19103327333928277 1106 4 0.20217075210886293 1106 5 0.17108142890507594 1106 6 0.37847467247062383 1106 7 0.2957719740914655 1106 8 0.19082911304930406 1106 9 0.21013592722160013 1106 10-11 0.17553013836521814 1106 12-13 0.22711712764167657 1106 14-15 0.5608392299081331 1106 16-17 0.5021933506002156 1106 18-19 0.31399999832240155 1106 20-21 0.2503926230603142 1106 22-23 0.4859770671416257 1106 24-25 0.5140528713781052 1106 26-27 0.46172716441538597 1106 28-29 0.5744569594978941 1106 30-31 0.39349014162505824 1106 32-33 0.5532011659052927 1106 34-35 0.7586618646903389 1106 36-37 0.6098135279870078 1106 38-39 0.5019964406267476 1106 40-41 0.5756747244580964 1106 42-43 0.6001364739560628 1106 44-45 0.5729652393525626 1106 46-47 0.5731485617750067 1106 48-49 0.6589760196140801 1106 50-51 0.3564688173885102 1106 52-53 0.5966349703933957 1106 54-55 0.5936350228488649 1106 56-57 1.741837941893241 1106 58-59 -8.635381274401244 1106 60-61 -9.85491994848843 1106 62-63 -3.6095157920614547 1106 64-65 -5.628455217558017 1106 66-67 -6.5669209294646755 1106 68-69 -3.693444070951273 1106 70-71 -4.032063964905596 1106 72-73 -4.342312743349279 1106 74-75 -4.95503193751969 1106 76-77 -6.449277281930396 1106 78-79 -8.612884146583426 1106 80-81 -4.752643027377459 1106 82-83 -2.540457513589949 1106 84-85 -2.075197677627557 1106 86-87 -2.135972478437857 1106 88-89 -1.9891672694814666 1106 90-91 -4.195226454984343 1106 92-93 -5.912107897827159 1106 94-95 -6.835371207734431 1106 96-97 -7.302859020785597 1106 98-99 -6.588849718632236 1106 100-101 -6.798420015122171 1107 1 -0.07052805457427525 1107 2 -0.11677026161848758 1107 3 -0.013587819485600505 1107 4 0.0966251914242946 1107 5 -0.036866086249965235 1107 6 -0.16206272381239017 1107 7 0.014688040719633477 1107 8 0.035786919920695937 1107 9 0.09817601380996166 1107 10-11 0.0371075921522106 1107 12-13 0.0035238768802940967 1107 14-15 -0.15823804729511437 1107 16-17 -0.1826200315394786 1107 18-19 0.07154095536476746 1107 20-21 0.09509656482507012 1107 22-23 -0.003920680480725025 1107 24-25 0.07087045828847494 1107 26-27 -0.04992777847535024 1107 28-29 0.20126652742861495 1107 30-31 0.15168217333533818 1107 32-33 -0.0908612574885197 1107 34-35 -0.05284973374575941 1107 36-37 -0.323816743037888 1107 38-39 -0.17926199903769913 1107 40-41 -0.07748612463966253 1107 42-43 -0.007950646233062741 1107 44-45 -0.07795112642924806 1107 46-47 0.009233970140506642 1107 48-49 -0.04165718893340653 1107 50-51 0.07594081120599583 1107 52-53 0.22389493075554157 1107 54-55 -0.21757077408398118 1107 56-57 1.098596113565879 1107 58-59 -9.344920951730796 1107 60-61 -10.735105589944475 1107 62-63 -4.755706776733987 1107 64-65 -6.0682160608389 1107 66-67 -7.342443513487378 1107 68-69 -4.893632703943766 1107 70-71 -4.539339630834073 1107 72-73 -3.2028543776422573 1107 74-75 -5.022604928931486 1107 76-77 -6.775148255301566 1107 78-79 -8.047020485856898 1107 80-81 -3.6595954411734297 1107 82-83 -2.6762811625165277 1107 84-85 -2.3742445408769477 1107 86-87 -2.1209400078027016 1107 88-89 -1.7208418235555634 1107 90-91 -1.6443661805108754 1107 92-93 -3.4023309750378843 1107 94-95 -5.857450408661807 1107 96-97 -6.281622435956842 1107 98-99 -5.8709707179716055 1107 100-101 -6.235853692343699 1108 1 -0.048543153638313186 1108 2 -0.02002764711222582 1108 3 0.05127329476800924 1108 4 0.05496248430239348 1108 5 0.12458076911729421 1108 6 -0.13578265955463564 1108 7 -0.12026078687802055 1108 8 -0.02186329766571049 1108 9 -0.061506015890394394 1108 10-11 -0.19632190155400053 1108 12-13 -0.039580104536241834 1108 14-15 0.028393775377615782 1108 16-17 -0.041516562978266336 1108 18-19 -0.12206796228424821 1108 20-21 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10.495053685224653 2316 96-97 12.197398670302258 2316 98-99 12.025208321329705 2316 100-101 10.854127657729082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 35.0 5 123.0 6 355.0 7 511.0 8 850.0 9 1241.0 10 2145.0 11 3250.0 12 4547.0 13 6423.0 14 7741.0 15 10381.0 16 14037.0 17 18397.0 18 20315.0 19 20115.0 20 21048.0 21 21131.0 22 21065.0 23 18439.0 24 17890.0 25 20332.0 26 28432.0 27 42476.0 28 59389.0 29 77618.0 30 93632.0 31 99355.0 32 97025.0 33 87110.0 34 77507.0 35 66307.0 36 48489.0 37 27559.0 38 9064.0 39 505.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.91076724545613 19.19444300847985 14.572838825419254 18.321950920644767 2 12.81646584308099 25.393072806645606 39.58255366435645 22.207907685916954 3 23.509352556544112 31.324077760592928 31.41988116931841 13.746688513544553 4 10.577861745012692 16.632116318866213 42.16281348937457 30.627208446746522 5 9.959404133954857 43.90592415971541 34.4301053422473 11.704566364082433 6 22.67804435460716 39.63269439116736 19.869895449307652 17.819365804917826 7 22.57496755521356 39.34394536259681 21.500269894683647 16.580817187505982 8 20.417404021097987 31.79513781549119 22.913290947217703 24.874167216193115 9 24.965066822861036 22.477058951889838 23.755996064514402 28.801878160734727 10-11 21.156537002369713 25.809253367941103 29.0987623127486 23.935447316940582 12-13 31.413779176380963 24.377136666245555 26.41402801545099 17.79505614192249 14-15 26.85218107035271 25.610654237361135 31.13684598396409 16.400318708322068 16-17 17.258616531336497 29.603809917576847 33.911166366145636 19.226407184941024 18-19 16.36975816157431 33.59499756185215 33.44358834110717 16.591655935466363 20-21 18.346426096710346 31.069328479057322 29.056603231845273 21.527642192387056 22-23 17.525268017325 29.42192097706031 27.518439276124724 25.53437172948997 24-25 20.156108233510583 29.904809484979687 30.432300459778034 19.50678182173169 26-27 23.16087325411626 25.583707908451974 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18.26604301509925 30.3771508268045 33.446428451692064 17.91037770640418 54-55 15.223658589614288 29.237962241959103 34.82121392360084 20.71716524482577 56-57 18.81429180694335 33.14879548983799 28.420432435658853 19.616480267559815 58-59 17.822963306407864 29.371172024109832 30.633586111304368 22.172278558177936 60-61 18.361734837494318 27.467249853842326 31.702526422661535 22.468488886001825 62-63 24.955302621352903 26.531196281790653 29.66695188762677 18.846549209229675 64-65 22.054289900710007 27.898609403464658 32.94743984000138 17.099660855823956 66-67 18.826221112067337 28.158714181599493 33.124585380093265 19.890479326239905 68-69 20.23949609256715 31.78676651177655 29.405170653665238 18.56856674199107 70-71 19.993782657061807 30.75131317968318 27.898429702148874 21.356474461106135 72-73 18.78291156676768 28.88159707190366 27.433734109961037 24.901757251367627 74-75 19.904997642045387 30.031985104568566 28.79822505882669 21.264792194559355 76-77 19.81792798726924 27.600313994314686 31.850701143864192 20.731056874551886 78-79 17.774254745822105 27.14269487777469 33.94347305968075 21.139577316722452 80-81 17.196557116604307 33.32157040376695 30.72379843350338 18.758074046125365 82-83 17.461589002445805 31.846039218050816 28.604666409213568 22.087705370289818 84-85 18.382773420695113 28.03314924001089 28.690659572828842 24.893417766465152 86-87 20.268900614156905 28.877098752257506 30.414118047905582 20.439882585680007 88-89 22.826136370705665 25.051813632668026 31.857380553485882 20.26466944314043 90-91 18.35538393183587 27.166187013769672 34.03912201928272 20.439307035111742 92-93 15.79205360901634 31.485205041800345 32.50810728723537 20.21463406194794 94-95 16.9986092229143 31.758634204124636 28.886234825331886 22.35652174762918 96-97 17.18735810759402 29.409015828136237 27.23538983749715 26.168236226772596 98-99 17.7861536354018 28.826967562793833 28.712955937202768 24.6739228646016 100-101 20.601673177873142 29.328317054108826 29.113575321762188 20.95643444625584 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7114.0 1 5061.0 2 2844.5 3 2725.0 4 2768.5 5 3018.0 6 3956.5 7 5479.0 8 6525.0 9 6763.0 10 6536.0 11 6097.0 12 5887.5 13 5673.0 14 5388.5 15 5086.0 16 4669.0 17 4236.0 18 3865.5 19 3581.0 20 3396.0 21 3376.5 22 3387.5 23 3484.0 24 3700.0 25 4171.5 26 4970.0 27 5977.0 28 6952.0 29 8109.5 30 9759.0 31 11797.0 32 14858.5 33 18657.5 34 22656.0 35 26418.5 36 30033.5 37 32914.5 38 33347.5 39 31891.5 40 29782.5 41 28075.0 42 27522.0 43 29805.5 44 35959.5 45 45541.0 46 59611.5 47 77004.0 48 94971.5 49 86061.0 50 55772.5 51 34049.0 52 19328.5 53 10882.5 54 6500.5 55 4500.0 56 3638.5 57 3301.5 58 3198.0 59 3113.5 60 2970.5 61 2667.0 62 2090.5 63 1446.0 64 929.0 65 629.0 66 431.0 67 301.0 68 234.5 69 191.0 70 163.5 71 142.5 72 138.0 73 124.5 74 85.5 75 61.5 76 34.0 77 11.0 78 5.0 79 2.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024692492333842495 2 0.006795220758538051 3 3.82829338509186E-4 4 0.0 5 0.1336074391397059 6 0.0 7 0.0 8 0.0012441953501548544 9 0.0 10-11 0.0 12-13 0.0 14-15 9.092196789593168E-4 16-17 0.0 18-19 1.4356100194094475E-4 20-21 0.0 22-23 3.3497567119553773E-4 24-25 0.0 26-27 1.4356100194094475E-4 28-29 0.003684732383150915 30-31 0.012585514503489489 32-33 0.0024405370329960607 34-35 0.004689659396737529 36-37 0.10685723911137654 38-39 0.10561304376122169 40-41 0.11738504592037914 42-43 0.11953846094949333 44-45 0.11618870423753797 46-47 0.10982416648482272 48-49 0.10958489814825449 50-51 0.10767075145570856 52-53 0.08546664982217578 54-55 0.07493884301317316 56-57 0.5867816685999548 58-59 1.640280154509921 60-61 0.5488337104202318 62-63 0.004593952062110232 64-65 0.1285349504044592 66-67 1.9687477269508025 68-69 2.968362983465601 70-71 2.8663389647529027 72-73 2.726893378200932 74-75 2.078715454437566 76-77 1.4867177361004238 78-79 0.994351353110297 80-81 0.05847718145727816 82-83 1.4356100194094475E-4 84-85 1.4356100194094475E-4 86-87 4.7853667313648254E-4 88-89 1.4356100194094475E-4 90-91 0.26597068292925696 92-93 0.42398349239892347 94-95 2.07522213672367 96-97 2.512748216972356 98-99 1.4902589074816337 100-101 0.9909058890637142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1044852.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.220269823473165 #Duplication Level Percentage of deduplicated Percentage of total 1 71.82273220758312 10.931613636584716 2 11.003984959631435 3.349672404380618 3 4.460801852929755 2.036838234919198 4 2.537968493333979 1.5451426108806725 5 1.5775551281206106 1.2005407355699735 6 1.0918929831979418 0.9971343493577836 7 0.8273491577835853 0.8814734193782607 8 0.6749153277970267 0.8217914717654867 9 0.5247311562794413 0.7187894805020547 >10 4.17125312608821 13.318470700196642 >50 0.7122365274012985 7.600081402391906 >100 0.514043500783446 14.880025308366132 >500 0.03649268426614427 3.73008543911605 >1k 0.030200842151291808 9.340870935709447 >5k 0.007550210537822952 9.086139933474621 >10k+ 0.006291842114852461 19.56132993740644 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 44137 4.224234628444985 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 38146 3.6508519866928526 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 23464 2.2456768996948853 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 17773 1.7010064583309406 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 17010 1.6279817620103134 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 15246 1.4591540237277625 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 14155 1.3547373216493819 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12043 1.1526034309165318 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11529 1.1034098609181013 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 10764 1.0301937499282194 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9807 0.9386018306898968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9586 0.9174505097372643 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 9464 0.9057742149127341 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 8826 0.8447129354205188 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7875 0.7536952601899599 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 7830 0.7493884301317316 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7828 0.749197015462477 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 7580 0.7254615964749075 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 7207 0.6897627606589258 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6884 0.6588492915743092 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 6375 0.6101342582490151 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 5619 0.537779513270779 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4529 0.4334585185270258 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 4454 0.42628046842997863 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4392 0.42034661368308623 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4054 0.38799753457906 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 3661 0.35038455207053243 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3596 0.3441635753197582 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3273 0.31325010623514143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3180 0.30434932411480287 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2845 0.2722873670146585 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2841 0.2719045376761493 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2837 0.27152170833764017 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2564 0.24539360598438822 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2456 0.23505721384464018 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2288 0.2189783816272544 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2286 0.21878696695799982 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2081 0.19916696335940404 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2075 0.19859271935164022 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2029 0.1941901819587846 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2016 0.19294598660862974 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1973 0.188830571219656 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1925 0.18423661915754574 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1910 0.18280100913813632 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1864 0.17839847174528067 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1696 0.16231963952789485 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1655 0.15839563880817573 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1625 0.1555244187693568 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1567 0.1499733933609736 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1558 0.14911202734932796 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1545 0.14786783199917308 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1487 0.1423168065907899 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1485 0.1421253919215353 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1412 0.13513875649374266 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1403 0.134277390482097 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1337 0.12796070639669543 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1291 0.12355816900383979 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1251 0.11972987561874793 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1172 0.11216899618319151 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1160 0.11102050816766394 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1147 0.10977631281750909 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1141 0.10920206880974531 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1090 0.10432099474375318 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1083 0.10365104340136211 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1079 0.10326821406285293 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1059 0.101354067370307 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1059 0.101354067370307 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1059 0.101354067370307 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.570733462729649E-5 0.0 10-11 0.0 0.0 0.0 2.871220038818895E-4 0.0 12-13 0.0 0.0 0.0 6.220976750774272E-4 0.0 14-15 0.0 0.0 0.0 8.613660116456685E-4 0.0 16-17 0.0 0.0 0.0 0.0017705856906049852 0.0 18-19 0.0 0.0 0.0 0.0022491223637414676 0.0 20-21 0.0 0.0 0.0 0.0030147810407598393 0.0 22-23 0.0 0.0 0.0 0.006795220758538051 0.0 24-25 0.0 0.0 0.0 0.011389172820648283 0.0 26-27 0.0 0.0 0.0 0.0163659542212677 0.0 28-29 0.0 0.0 0.0 0.03124844475581231 0.0 30-31 0.0 0.0 0.0 0.06388464586372042 0.0 32-33 0.0 0.0 0.0 0.11896421694172954 0.0 34-35 0.0 0.0 0.0 0.233860872161799 0.0 36-37 0.0 0.0 0.0 0.4035978301233093 0.0 38-39 0.0 0.0 0.0 0.5720427390673513 0.0 40-41 0.0 0.0 0.0 0.7600597979426751 0.0 42-43 0.0 0.0 0.0 0.9478854421487445 0.0 44-45 0.0 0.0 0.0 1.133126988319877 0.0 46-47 0.0 0.0 0.0 1.3806261556660657 0.0 48-49 0.0 0.0 0.0 1.6344898607649696 0.0 50-51 0.0 0.0 0.0 1.8333218484531781 0.0 52-53 0.0 0.0 0.0 1.9967899759966004 0.0 54-55 0.0 0.0 0.0 2.154419956127758 0.0 56-57 0.0 0.0 0.0 2.279700857154889 0.0 58-59 0.0 0.0 0.0 2.405412441187843 0.0 60-61 0.0 0.0 0.0 2.5451930034110095 0.0 62-63 0.0 9.570733462729649E-5 0.0 2.6481262418026668 0.0 64-65 0.0 9.570733462729649E-5 0.0 2.7082304479486092 0.0 66-67 0.0 9.570733462729649E-5 0.0 2.759816701312722 0.0 68-69 0.0 9.570733462729649E-5 0.0 2.829013104248257 0.0 70-71 0.0 9.570733462729649E-5 0.0 2.943479076462504 0.0 72-73 0.0 9.570733462729649E-5 0.0 3.1470007235474498 0.0 74-75 0.0 9.570733462729649E-5 0.0 3.3853598404367315 0.0 76-77 0.0 9.570733462729649E-5 0.0 3.669227794941293 0.0 78-79 0.0 9.570733462729649E-5 0.0 4.12422046375946 0.0 80-81 0.0 9.570733462729649E-5 0.0 4.687218859704533 0.0 82-83 0.0 9.570733462729649E-5 0.0 5.217341786205127 0.0 84-85 0.0 9.570733462729649E-5 0.0 5.8514507317782805 0.0 86-87 0.0 9.570733462729649E-5 0.0 6.373869217841378 0.0 88-89 0.0 9.570733462729649E-5 0.0 6.680180542316041 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCGG 15 0.009573007 48.419434 94-95 GACGCGG 55 2.5465852E-11 44.220505 70-71 CAATCGG 50 2.7326678E-8 38.45202 54-55 GACATAT 265 0.0 37.28155 1 CGCGCCG 40 9.209234E-6 36.453014 72-73 ACGCACG 35 1.7895573E-4 34.733494 62-63 GCAACTC 485 0.0 34.628857 68-69 GGCATTC 95 9.483865E-7 34.615536 7 GCGACTC 65 6.526534E-9 33.70233 68-69 TAGCGAC 90 3.6379788E-12 32.460537 66-67 CGTTCGG 30 0.0036689867 32.150387 88-89 GTAGCGA 115 0.0 31.754873 64-65 AGCAACT 560 0.0 30.866491 66-67 GTAGCAA 1280 0.0 30.241552 64-65 GAACCCG 95 3.7638332E-5 29.67046 9 CTGCGGG 355 0.0 29.45009 62-63 CGCGACG 50 4.190858E-5 29.208689 68-69 AGTGGTT 510 0.0 29.200266 60-61 AACTCGA 50 4.213592E-5 29.185534 70-71 ACTGCGG 460 0.0 29.070423 62-63 >>END_MODULE