FastQCFastQC Report
Fri 27 May 2016
SRR939705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940359
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147340.5010952744205724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100900.3431553766053737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72700.24724872030932277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA177900.032.2249641
GGTATCA116400.030.4149671
GTACATG139250.028.5253981
TACATGG143100.027.3268172
TCAACGC214500.026.728264
ATCAACG214250.026.6690253
TATCAAC214250.026.6247232
CAACGCA215900.026.5338615
AACGCAG219900.026.0687986
ACATGGG143500.025.8285853
CATGGGA96250.023.5206174
ACGCAGA243350.023.3422167
CGCAGAG243350.023.1487878
GAGTACT110100.022.67291312-13
AGTACTT116800.021.47390412-13
GCAGAGT246700.021.4501029
ATGGGAT44800.020.6586745
GTACTTT123800.020.12622514-15
AGAGTAC194900.019.85801310-11
CATGGGG51900.019.5693994
Produced by FastQC (version 0.11.5)