FastQCFastQC Report
Fri 27 May 2016
SRR939705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940359
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154490.5254120330204577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117890.400937436551115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85790.29176709374603577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111100.033.1752851
GTATCAA167400.030.706771
GTACATG134100.027.0640371
TACATGG134950.026.434712
ACATGGG133200.025.6496263
ATCAACG202250.025.2225743
TCAACGC202550.025.1619834
CAACGCA205400.024.8586735
TATCAAC207050.024.7077122
AACGCAG207750.024.5913816
CATGGGA86150.022.5055984
CATGGGG47400.022.437724
ACGCAGA234100.021.6226397
CGCAGAG232250.021.5321648
GAGTACT116450.020.78593412-13
GCAGAGT240650.020.0579419
AGTACTT124550.020.0001812-13
AGAGTAC199000.019.46148310-11
ATGGGAT39200.019.2080175
GTACTTT128100.018.9137514-15
Produced by FastQC (version 0.11.5)