##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939705_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2940359 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55624840368132 31.0 31.0 34.0 30.0 34.0 2 31.767000560135685 31.0 31.0 34.0 30.0 34.0 3 31.805260174012766 31.0 31.0 34.0 30.0 34.0 4 35.5940019569039 37.0 35.0 37.0 33.0 37.0 5 35.33732309558118 37.0 35.0 37.0 33.0 37.0 6 35.36133342901326 37.0 35.0 37.0 33.0 37.0 7 35.29056451950255 37.0 35.0 37.0 32.0 37.0 8 35.257865451123486 37.0 35.0 37.0 32.0 37.0 9 36.85881757975812 39.0 37.0 39.0 33.0 39.0 10-11 36.74548158915289 39.0 36.0 39.0 32.0 39.0 12-13 36.688105772118305 39.0 35.0 39.0 32.0 39.0 14-15 37.73167069055173 39.5 37.0 41.0 32.0 41.0 16-17 37.70222547654895 39.0 37.0 41.0 32.0 41.0 18-19 37.71445221484859 39.5 37.0 41.0 32.0 41.0 20-21 37.66360621271076 39.0 37.0 41.0 32.0 41.0 22-23 37.52667225328608 39.0 36.0 41.0 32.0 41.0 24-25 37.40220939007788 39.0 36.0 41.0 32.0 41.0 26-27 37.13928690340193 39.0 36.0 40.5 31.0 41.0 28-29 36.94306409523463 39.0 36.0 40.0 31.0 41.0 30-31 36.69104384872732 38.5 35.5 40.0 30.0 41.0 32-33 36.49650297803772 38.0 35.0 40.0 30.0 41.0 34-35 36.46921872465233 38.0 35.0 40.0 30.0 41.0 36-37 36.346819895121655 38.0 35.0 40.0 30.0 41.0 38-39 36.36958004107662 38.5 35.0 40.0 30.0 41.0 40-41 36.2693399003319 39.0 35.0 40.0 30.0 41.0 42-43 36.070765338518186 38.0 35.0 40.0 29.5 41.0 44-45 35.82952965947355 38.0 35.0 40.0 29.0 41.0 46-47 35.49358598728931 38.0 34.5 40.0 27.5 41.0 48-49 35.16684459278612 38.0 34.0 40.0 26.5 41.0 50-51 34.91132409341853 38.0 34.0 40.0 26.0 41.0 52-53 34.68098861397537 38.0 34.0 40.0 25.5 41.0 54-55 34.39974183424541 38.0 33.0 40.0 24.0 41.0 56-57 32.41650509342567 37.0 30.5 39.5 15.5 41.0 58-59 27.548419597742996 33.0 17.5 38.5 6.5 40.0 60-61 28.304741359813548 33.0 20.0 38.0 7.5 40.0 62-63 31.281497599442787 35.0 29.5 38.5 8.5 40.0 64-65 31.49053006792708 35.5 30.5 39.0 4.5 40.0 66-67 24.851562173190416 27.0 18.0 35.5 2.0 39.0 68-69 17.463447320548273 13.0 7.5 28.0 2.0 35.5 70-71 16.348307468577815 12.5 7.5 27.0 2.0 34.0 72-73 17.496950032291977 13.5 7.0 28.5 2.0 34.0 74-75 18.49839815478314 17.5 8.5 30.0 2.0 34.5 76-77 19.690696952310923 21.5 8.0 30.0 2.0 34.5 78-79 22.785067061539085 27.5 10.5 33.5 2.0 36.0 80-81 26.32409426876106 31.5 22.5 35.0 2.0 36.0 82-83 27.579305622204636 33.5 26.5 35.0 2.0 36.0 84-85 27.6166559933668 34.0 27.0 35.0 2.0 36.0 86-87 27.368197556828946 34.0 26.5 35.0 2.0 36.0 88-89 26.948003288033874 33.0 26.0 35.0 2.0 35.5 90-91 25.633599332598504 32.5 18.5 35.0 2.0 35.0 92-93 23.195763680557377 31.5 2.0 34.5 2.0 35.0 94-95 16.001704553763673 7.0 2.0 32.0 2.0 35.0 96-97 13.473197150415988 2.0 2.0 30.0 2.0 34.0 98-99 13.15379159483587 2.0 2.0 30.0 2.0 34.0 100-101 12.854846976168556 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3322665891068226 1101 2 0.37378967837824817 1101 3 0.392033161252904 1101 4 0.19880350238394584 1101 5 0.2575889282774213 1101 6 0.4199626984157092 1101 7 0.37324856283851204 1101 8 0.30339931327344516 1101 9 0.4452022351414868 1101 10-11 0.5124554020057204 1101 12-13 0.5539088096638025 1101 14-15 0.7141202603368129 1101 16-17 0.6848187023436125 1101 18-19 0.5779759654683758 1101 20-21 0.4656653821226584 1101 22-23 0.5974421571910895 1101 24-25 0.5036686264062311 1101 26-27 0.5265741129219563 1101 28-29 0.5826446576374025 1101 30-31 0.6048534006875101 1101 32-33 0.5718244314918834 1101 34-35 0.43303257611340484 1101 36-37 0.290233868853754 1101 38-39 0.3148359890166361 1101 40-41 0.4844716858801945 1101 42-43 0.3651147978721241 1101 44-45 0.09838248815851358 1101 46-47 0.20364802994055964 1101 48-49 0.1934475662835453 1101 50-51 0.12270069065636591 1101 52-53 0.14775679913960005 1101 54-55 0.03805698502378618 1101 56-57 -2.2702345895155247 1101 58-59 -9.552642280301288 1101 60-61 -9.789300673396333 1101 62-63 -3.914718574768351 1101 64-65 -5.26503220156113 1101 66-67 -10.007155259895583 1101 68-69 -5.054067908188252 1101 70-71 -4.586451898221853 1101 72-73 -4.697958266391744 1101 74-75 -6.783973831751279 1101 76-77 -7.9188176389709515 1101 78-79 -11.298654186387338 1101 80-81 -8.306463117442568 1101 82-83 -4.528199260273688 1101 84-85 -3.628747000023857 1101 86-87 -3.3505558325022164 1101 88-89 -3.4574735405446937 1101 90-91 -9.7013039414655 1101 92-93 -16.928900937165775 1101 94-95 -11.057265024636095 1101 96-97 -9.129297111738701 1101 98-99 -9.165239084171391 1101 100-101 -9.086615164622263 1102 1 0.34845043037634227 1102 2 0.30285421941861657 1102 3 0.36228160665523745 1102 4 0.29079133059081386 1102 5 0.30606915763105746 1102 6 0.29194782626622384 1102 7 0.41730948237294285 1102 8 0.47744453540930465 1102 9 0.5123398447961449 1102 10-11 0.4421814672584432 1102 12-13 0.3389255859430591 1102 14-15 0.41099832032005423 1102 16-17 0.4285509418223228 1102 18-19 0.4260671983955504 1102 20-21 0.46454296765076464 1102 22-23 0.506637312196581 1102 24-25 0.555586447243904 1102 26-27 0.44519527816357396 1102 28-29 0.4784282530987838 1102 30-31 0.431418913351429 1102 32-33 0.5399726873645179 1102 34-35 0.26130663199815984 1102 36-37 0.20825833736305555 1102 38-39 0.15335231355403778 1102 40-41 0.3515703044081775 1102 42-43 0.4935869324659592 1102 44-45 0.474276034811588 1102 46-47 0.43475231795500946 1102 48-49 0.24898452709557262 1102 50-51 0.467167578483064 1102 52-53 0.317734609529154 1102 54-55 0.0936343249961169 1102 56-57 -2.8385386206175696 1102 58-59 -10.56335789996998 1102 60-61 -10.811054630554043 1102 62-63 -4.568728635488515 1102 64-65 -5.279488256416219 1102 66-67 -6.452052217325853 1102 68-69 -4.185597029133934 1102 70-71 -4.186242999159656 1102 72-73 -4.700793640555137 1102 74-75 -5.49577688113499 1102 76-77 -7.773556291165054 1102 78-79 -10.28262113604462 1102 80-81 -6.024825414411104 1102 82-83 -3.5950302406797014 1102 84-85 -3.1074474661507914 1102 86-87 -3.072856043988068 1102 88-89 -3.103317073822165 1102 90-91 -8.878685964915697 1102 92-93 -13.253138969792136 1102 94-95 -10.262499047107097 1102 96-97 -9.126738082453715 1102 98-99 -9.117142772302946 1102 100-101 -9.087793573632872 1103 1 0.20865676788555376 1103 2 0.2167377861098494 1103 3 0.22418285941868277 1103 4 0.1774530844596356 1103 5 0.19613548040187112 1103 6 0.152419454858709 1103 7 0.16010977714081065 1103 8 0.21915418905705764 1103 9 0.27500749402237545 1103 10-11 0.33873268317591254 1103 12-13 0.25664113494821805 1103 14-15 0.3800182171512958 1103 16-17 0.31304615184442497 1103 18-19 0.3503487017116882 1103 20-21 0.1923469470170147 1103 22-23 0.2995407757190591 1103 24-25 0.34257244577005963 1103 26-27 0.2856079531820015 1103 28-29 0.3111032715217732 1103 30-31 0.28983453604855214 1103 32-33 0.379235767688769 1103 34-35 0.4317193070165857 1103 36-37 0.26182502859371226 1103 38-39 0.3044945390514613 1103 40-41 0.39784149084885456 1103 42-43 0.3131521498572596 1103 44-45 0.30843226178284766 1103 46-47 0.4513919642335722 1103 48-49 0.29258806431001005 1103 50-51 0.1624291849679551 1103 52-53 0.3357375572078496 1103 54-55 0.3458229764331122 1103 56-57 -2.8182585911407827 1103 58-59 -10.591272417287591 1103 60-61 -10.710406141239378 1103 62-63 -4.495139836667587 1103 64-65 -5.6879480942938905 1103 66-67 -6.830659439138675 1103 68-69 -4.328087817637986 1103 70-71 -4.390325534163342 1103 72-73 -4.8555099264799715 1103 74-75 -5.415172607000873 1103 76-77 -7.013895274215901 1103 78-79 -9.496792101409396 1103 80-81 -5.483410528633655 1103 82-83 -3.3220015008123447 1103 84-85 -2.8800119466224174 1103 86-87 -2.6360837521678775 1103 88-89 -2.5657562779489176 1103 90-91 -5.4949571513563775 1103 92-93 -10.6473467811407 1103 94-95 -8.609912459045194 1103 96-97 -8.111999688938607 1103 98-99 -8.203878218139351 1103 100-101 -8.247646189697722 1104 1 0.29780201294418873 1104 2 0.2994588861799663 1104 3 0.2406468545842131 1104 4 0.15118731399437735 1104 5 0.1468226251566449 1104 6 0.1910173443386043 1104 7 0.2415690119490037 1104 8 0.31457797488088346 1104 9 0.26369984951560355 1104 10-11 0.2656362845145068 1104 12-13 0.41888329176065753 1104 14-15 0.4497705075387941 1104 16-17 0.40345012660028345 1104 18-19 0.3486184400915775 1104 20-21 0.3115860309760379 1104 22-23 0.4224322794788904 1104 24-25 0.4576921958370477 1104 26-27 0.47241098840864737 1104 28-29 0.45952271886424256 1104 30-31 0.5392580482230969 1104 32-33 0.5489702708727933 1104 34-35 0.5571319188279702 1104 36-37 0.4333906445611433 1104 38-39 0.5396228406585806 1104 40-41 0.35751932582693513 1104 42-43 0.48134156077017565 1104 44-45 0.5243204532461263 1104 46-47 0.5347405193083361 1104 48-49 0.411145647530752 1104 50-51 0.4339735361055972 1104 52-53 0.5154400206215186 1104 54-55 0.431459695606236 1104 56-57 -2.701647053402837 1104 58-59 -10.219738613049447 1104 60-61 -10.278538142812863 1104 62-63 -3.9711149117786135 1104 64-65 -5.188567909922686 1104 66-67 -6.413690504033461 1104 68-69 -4.3321121154140165 1104 70-71 -4.270411904718678 1104 72-73 -4.596609483510193 1104 74-75 -5.210745930756479 1104 76-77 -6.710907708155254 1104 78-79 -9.496712971158036 1104 80-81 -5.138599318134645 1104 82-83 -2.8584182344660753 1104 84-85 -2.389691605929059 1104 86-87 -2.2862385350442764 1104 88-89 -2.2525431222595778 1104 90-91 -4.502619160284183 1104 92-93 -6.997190630017883 1104 94-95 -7.9353559134113265 1104 96-97 -7.766510341307099 1104 98-99 -8.020211374740853 1104 100-101 -8.099347262060853 1105 1 0.31488077509650125 1105 2 0.2746091870534322 1105 3 0.29050362113219563 1105 4 0.17391378650027178 1105 5 0.18956838263761 1105 6 0.24761185141525033 1105 7 0.3156531369637321 1105 8 0.2102945344295506 1105 9 0.35265708983272503 1105 10-11 0.432433714044933 1105 12-13 0.29863789458516266 1105 14-15 0.47565787508762014 1105 16-17 0.4439227613293397 1105 18-19 0.3673606077639846 1105 20-21 0.3987545938174435 1105 22-23 0.4732414072475635 1105 24-25 0.522577294904913 1105 26-27 0.6330634124521239 1105 28-29 0.6928920317976619 1105 30-31 0.6148350209620403 1105 32-33 0.6286252901345577 1105 34-35 0.6007501646288702 1105 36-37 0.5515657995491026 1105 38-39 0.5109652816536823 1105 40-41 0.338266560305577 1105 42-43 0.3114190120106173 1105 44-45 0.31269151364691083 1105 46-47 0.5812223586108516 1105 48-49 0.39774817133327645 1105 50-51 0.4034229723704712 1105 52-53 0.5620317913012656 1105 54-55 0.5382198752687515 1105 56-57 1.576600527229452 1105 58-59 -8.586933810757802 1105 60-61 -9.963507647894104 1105 62-63 -3.7596613982031073 1105 64-65 -4.9211911386378056 1105 66-67 -6.360057804124498 1105 68-69 -4.1253855944522755 1105 70-71 -4.0411813779413315 1105 72-73 -2.139853781485135 1105 74-75 -4.33420922652634 1105 76-77 -6.395198265276379 1105 78-79 -8.942061000199393 1105 80-81 -4.622397572886285 1105 82-83 -2.162848616147592 1105 84-85 -1.7482795504081636 1105 86-87 -1.608902354590164 1105 88-89 -1.5805172726491001 1105 90-91 -4.019591840355684 1105 92-93 -6.190119687598035 1105 94-95 -7.737667880092722 1105 96-97 -8.020917247941169 1105 98-99 -8.494640045749755 1105 100-101 -8.437038621624424 1106 1 0.22983070216143275 1106 2 0.18276634835004302 1106 3 0.16541944481358684 1106 4 0.15699975716113812 1106 5 0.09740255759614769 1106 6 0.24601903559646132 1106 7 0.2609294824661532 1106 8 0.2188445067196696 1106 9 0.27407916166175994 1106 10-11 0.2930441759638782 1106 12-13 0.24106661465807377 1106 14-15 0.28484497370524764 1106 16-17 0.39572228367381257 1106 18-19 0.4259618131854239 1106 20-21 0.3300647072990657 1106 22-23 0.36184683715527655 1106 24-25 0.3890433773183517 1106 26-27 0.2578801877782837 1106 28-29 0.30855804003422804 1106 30-31 0.3712110740382357 1106 32-33 0.3268502608221624 1106 34-35 0.4431835089294651 1106 36-37 0.3321950989868867 1106 38-39 0.32019483801402515 1106 40-41 0.26512724427024636 1106 42-43 0.24721184708322141 1106 44-45 0.27876588494708443 1106 46-47 0.22138636612978502 1106 48-49 0.29271705891439126 1106 50-51 0.28503694846593675 1106 52-53 0.23133375526161615 1106 54-55 0.1867180774967494 1106 56-57 1.5915789447406823 1106 58-59 -8.471581623190612 1106 60-61 -9.972431106400116 1106 62-63 -3.5187246479400223 1106 64-65 -5.092678730214661 1106 66-67 -6.898839936297936 1106 68-69 -3.978294351935304 1106 70-71 -4.014601175265334 1106 72-73 -4.238441665346295 1106 74-75 -4.9776644778395145 1106 76-77 -6.484530361224728 1106 78-79 -9.175188423336095 1106 80-81 -4.610016410215891 1106 82-83 -2.1973603154436425 1106 84-85 -1.6918397870645485 1106 86-87 -1.5576799263999241 1106 88-89 -1.5385098303626528 1106 90-91 -3.9970114172305706 1106 92-93 -6.144223872985332 1106 94-95 -7.162419218457751 1106 96-97 -7.175690030422938 1106 98-99 -6.509407238947622 1106 100-101 -7.259078589616728 1107 1 0.048414825319468946 1107 2 -0.06448663647731223 1107 3 -0.05895031876696777 1107 4 0.08693209561270976 1107 5 -0.007764361523129537 1107 6 0.0016776001120675232 1107 7 0.024769696346922387 1107 8 0.09453291040595957 1107 9 0.13067272630480176 1107 10-11 0.07516611603794132 1107 12-13 0.057183749333042044 1107 14-15 0.0918839412226049 1107 16-17 -0.02175757169996473 1107 18-19 0.014414632158697316 1107 20-21 0.09163023058599151 1107 22-23 0.10058955941977388 1107 24-25 0.2136884113448403 1107 26-27 0.11792419099705143 1107 28-29 0.0355223459048446 1107 30-31 0.12474924141432098 1107 32-33 0.13125133368507846 1107 34-35 0.08541431009026468 1107 36-37 0.04611500839524041 1107 38-39 -0.11188543372450965 1107 40-41 -0.1687928289895595 1107 42-43 -0.1506876455234334 1107 44-45 -0.008435108903022126 1107 46-47 0.027095178425192046 1107 48-49 -0.0881340310618981 1107 50-51 -0.0707935690497834 1107 52-53 -0.1270109718164747 1107 54-55 -0.21875351430193746 1107 56-57 1.0141949743286105 1107 58-59 -8.586116976873083 1107 60-61 -10.441085753955633 1107 62-63 -4.04887798104275 1107 64-65 -5.439747404911262 1107 66-67 -6.831827876830783 1107 68-69 -4.275881024804052 1107 70-71 -4.24588922521454 1107 72-73 -2.5092449971746227 1107 74-75 -4.775417733021772 1107 76-77 -6.790225519232148 1107 78-79 -7.685292255093421 1107 80-81 -2.708558723944094 1107 82-83 -1.4034787336216894 1107 84-85 -1.1371899785886974 1107 86-87 -1.0811812317498593 1107 88-89 -1.0471046357253293 1107 90-91 -0.48821327676254 1107 92-93 -3.1648385480678947 1107 94-95 -6.213137957420535 1107 96-97 -6.629538878365146 1107 98-99 -6.452016445942986 1107 100-101 -7.06569791534978 1108 1 -0.16370968392255492 1108 2 -0.1328224652368455 1108 3 -0.11708511224808049 1108 4 -0.1218980174784079 1108 5 -0.03800585078467833 1108 6 -0.20033331011700994 1108 7 -0.09458741276858262 1108 8 -0.08429345335202498 1108 9 -0.2313402526642463 1108 10-11 -0.15117065748746938 1108 12-13 -0.20594165223374716 1108 14-15 -0.1864208399203946 1108 16-17 -0.15664685075440588 1108 18-19 -0.21721017727772818 1108 20-21 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94-95 11.259046553340722 2316 96-97 13.077425425142225 2316 98-99 12.81208902436391 2316 100-101 11.610127706162773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 55.0 5 88.0 6 283.0 7 651.0 8 1478.0 9 2638.0 10 4351.0 11 7234.0 12 10809.0 13 14971.0 14 18930.0 15 25462.0 16 34959.0 17 46883.0 18 50067.0 19 48359.0 20 50042.0 21 49659.0 22 49676.0 23 42695.0 24 43068.0 25 50813.0 26 71537.0 27 106266.0 28 150294.0 29 202360.0 30 251797.0 31 276826.0 32 280895.0 33 261916.0 34 233499.0 35 212612.0 36 175077.0 37 115559.0 38 45170.0 39 3360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15732798576703 20.353680959160172 14.492755102214375 22.996235952858413 2 15.165821810088215 21.847399311054154 40.09490691612383 22.8918719627338 3 18.466994110232985 26.750180420452775 30.877030452795818 23.905795016518418 4 12.308020891326535 17.746030331670383 38.4354087375045 31.51054003949858 5 12.469788614807351 38.98684542566339 34.864134330792915 13.679231628736343 6 29.92488332207054 38.127691210495044 18.03324015876973 13.91418530866469 7 26.58097191533415 34.0388027448349 22.17368695455215 17.206538385278805 8 25.3012171120092 35.87550474931138 20.52857494130743 18.294703197371987 9 26.45557906364495 16.290833874367042 20.938157551509867 36.31542951047814 10-11 24.051586898062446 27.37141961236706 28.328377589267163 20.248615900303328 12-13 25.257000930838718 24.967631503500083 28.681089622049555 21.094277943611647 14-15 22.238441076387005 25.87977600522933 27.33248388407245 24.549299034311208 16-17 21.068141679298343 29.181334660155443 27.890965014816217 21.859558645729994 18-19 21.00592666834107 27.754605016029537 30.42627357560919 20.813194740020204 20-21 22.018025690060295 26.90836391066533 30.315243818867017 20.75836658040736 22-23 21.619062631840194 27.039557712442008 30.05222678460503 21.28915287111277 24-25 21.44989778459025 27.082696364627584 30.27531672152958 21.192089129252583 26-27 21.35942025929575 27.133955867143662 30.285826614459708 21.220797259100877 28-29 21.104497749263288 27.332961597437233 30.40039493112768 21.1621457221718 30-31 21.36558767096589 27.190868531051326 30.235014644622577 21.208529153360203 32-33 21.216367355552425 27.213212213328696 30.159921871373115 21.410498559745765 34-35 21.53839901055208 27.259048668205892 30.035298313003544 21.167254008238483 36-37 21.54959126094191 27.049648709950592 30.088199122519587 21.312560906587912 38-39 21.35541422177073 27.399901682161033 30.106365739637475 21.138318356430762 40-41 21.40993771690387 27.26089886087271 30.135376274270335 21.19378714795308 42-43 21.266965860753878 27.3645463518739 29.823312964483158 21.545174822889063 44-45 21.396067389429906 27.43100141891299 29.609505270065874 21.563425921591225 46-47 21.55996434129878 27.508284417804635 29.25036551530551 21.681385725591078 48-49 21.536506306670066 27.666074169803633 29.130827188490606 21.6665923350357 50-51 21.49166811072236 27.79897995764179 29.06404083437708 21.64531109725877 52-53 21.686532509188915 27.920152911137166 28.83427042064348 21.559044159030442 54-55 21.427042333093393 27.83666780680304 28.917449021439666 21.818840838663895 56-57 20.78053606560797 28.02231549126808 29.560331404914304 21.636817038209642 58-59 20.60589149483356 27.2672769697132 30.627272546643088 21.49955898881015 60-61 21.62462544079614 27.77442177289362 29.230027640033434 21.3709251462768 62-63 21.597783166623017 27.78423796777803 29.004533658931443 21.613445206667507 64-65 21.059543726740664 27.763577986477728 29.34145448274066 21.83542380404095 66-67 21.422783298503006 27.49364506909026 29.434590588495556 21.648981043911178 68-69 21.709505873865545 26.961016584105103 29.837398373983742 21.49207916804561 70-71 21.59608627028094 27.034129321447804 29.746109310538234 21.623675097733027 72-73 20.766805473244496 27.16140985004453 30.226892075833423 21.844892600877554 74-75 21.278785863444003 27.423611419653664 29.457695575869998 21.839907141032334 76-77 19.45213432587136 27.866888411225077 30.263630408257676 22.41734685464589 78-79 20.17030936421694 27.935871860451105 29.61294153413354 22.280877241198414 80-81 20.919904554096647 28.07130370427082 28.615651596932462 22.393140144700073 82-83 21.415014978656455 28.01557161297607 28.334020042848636 22.23539336551884 84-85 21.345453413865574 27.79940309918413 28.242904044393285 22.612239442557012 86-87 21.17887686480808 27.876158939609052 28.44563205836585 22.49933213721702 88-89 21.315698721990618 27.76388489277141 28.291848532523634 22.628567852714333 90-91 20.70912734589843 27.792150142954654 28.846905217623142 22.651817293523777 92-93 19.828962389241163 27.9771663048327 29.436605988842985 22.757265317083156 94-95 19.235197763731687 27.667642465705168 29.86675648946274 23.230403281100408 96-97 19.36301979930522 27.787093411793823 29.317526193684234 23.532360595216723 98-99 19.49306609631572 27.75541603422106 29.114012694908237 23.63750517455499 100-101 19.749497747481357 27.692168774699553 29.01504603144548 23.54328744637361 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 871.0 1 666.5 2 507.5 3 599.0 4 691.0 5 792.0 6 1029.0 7 1517.5 8 2066.5 9 2528.0 10 2847.0 11 3173.5 12 3574.0 13 4026.0 14 4560.5 15 5019.5 16 5426.0 17 5851.5 18 6383.5 19 7140.0 20 7930.5 21 8904.0 22 10346.5 23 12203.5 24 14536.0 25 17466.5 26 21369.0 27 25901.0 28 30433.5 29 35516.0 30 41389.5 31 47807.5 32 55310.0 33 63402.5 34 71509.5 35 79592.0 36 86853.0 37 92965.0 38 97201.0 39 99629.0 40 101395.5 41 102910.0 42 104170.0 43 105854.5 44 108129.0 45 109869.5 46 110857.0 47 111805.5 48 112421.5 49 110153.0 50 104868.0 51 99108.0 52 93713.0 53 87814.0 54 81146.5 55 73711.0 56 65619.5 57 57437.0 58 49455.5 59 41871.0 60 34116.5 61 26624.5 62 20288.5 63 15053.5 64 10841.5 65 7648.0 66 5223.5 67 3437.0 68 2175.5 69 1348.0 70 838.0 71 501.5 72 322.0 73 219.0 74 129.0 75 72.5 76 45.5 77 24.0 78 14.5 79 10.0 80 6.5 81 4.0 82 1.5 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023942654621425478 2 0.0073120323062592025 3 1.700472629362605E-4 4 0.0 5 0.13488148896104182 6 0.0 7 0.0 8 0.00129235919831558 9 0.0 10-11 0.0 12-13 0.0 14-15 7.652126832131722E-4 16-17 0.0 18-19 1.8705198922988655E-4 20-21 0.0 22-23 3.23089799578895E-4 24-25 0.0 26-27 3.40094525872521E-4 28-29 0.003383940532431584 30-31 0.01265151636245778 32-33 0.002703751480686542 34-35 0.0045402619203981556 36-37 0.10105908836301962 38-39 0.10022585677463194 40-41 0.1117550612017104 42-43 0.11420374178799256 44-45 0.1105817350874502 46-47 0.10432399581139583 48-49 0.10447703834803845 50-51 0.10304864133937387 52-53 0.08310209739695051 54-55 0.07189598276945094 56-57 0.5510211508186585 58-59 1.5794499923308682 60-61 0.5187801897659435 62-63 0.004387219383755521 64-65 0.11905008878167597 66-67 1.917384917964099 68-69 2.8878786569939248 70-71 2.7995901180774183 72-73 2.6439798677644464 74-75 2.0212157767129795 76-77 1.4123445470434053 78-79 0.9406844538370994 80-81 0.051745382111504076 82-83 2.890803469916429E-4 84-85 1.5304253664263445E-4 86-87 3.23089799578895E-4 88-89 1.700472629362605E-4 90-91 0.24694263523603752 92-93 0.3921119836047231 94-95 2.0109959362105103 96-97 2.442694922626795 98-99 1.4133988400736102 100-101 0.9430991249707943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2940359.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.23549028514875 #Duplication Level Percentage of deduplicated Percentage of total 1 70.59639769448881 33.34655457464524 2 14.868450467769401 14.046370952510737 3 5.73120221627523 8.121484398272749 4 2.8190662173357746 5.326398996886201 5 1.6198416465947447 3.825700718060271 6 0.9882144383847605 2.800727610238025 7 0.6707552432438033 2.217841694316868 8 0.46907866455561464 1.7725728562069827 9 0.35818866811819416 1.5227295617832597 >10 1.687189002797989 14.166787880596504 >50 0.1171210159704984 3.819372023522922 >100 0.06937708598247831 5.927038552511692 >500 0.0038202090083377348 1.1058146302417688 >1k 0.0010811912286200925 0.7811418165145607 >5k 7.207941524133951E-5 0.29208977221280635 >10k+ 1.4415883048267902E-4 0.9273739614794755 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15449 0.5254120330204577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11789 0.400937436551115 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8579 0.29176709374603577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.40094525872521E-5 0.0 14-15 0.0 0.0 0.0 5.101417888087815E-5 0.0 16-17 0.0 0.0 0.0 1.020283577617563E-4 0.0 18-19 0.0 0.0 0.0 1.700472629362605E-4 0.0 20-21 0.0 0.0 0.0 2.5507089440439076E-4 0.0 22-23 0.0 0.0 0.0 6.63184325451416E-4 0.0 24-25 0.0 0.0 0.0 0.0011903308405538235 0.0 26-27 0.0 0.0 0.0 0.0017344820819498571 0.0 28-29 0.0 0.0 0.0 0.003298916900963454 0.0 30-31 0.0 0.0 0.0 0.009131538019677189 0.0 32-33 0.0 0.0 0.0 0.022106144181713867 0.0 34-35 0.0 0.0 0.0 0.04074332419952802 0.0 36-37 0.0 0.0 0.0 0.06944730218316879 0.0 38-39 0.0 0.0 0.0 0.11328548656813675 0.0 40-41 0.0 0.0 0.0 0.1650138639533472 0.0 42-43 0.0 0.0 0.0 0.21682726497002577 0.0 44-45 0.0 0.0 0.0 0.2773640905753345 0.0 46-47 0.0 0.0 0.0 0.34898799772408745 0.0 48-49 0.0 0.0 0.0 0.4167178225516 0.0 50-51 0.0 1.700472629362605E-5 0.0 0.48317229290709063 0.0 52-53 0.0 3.40094525872521E-5 0.0 0.5496947821677557 0.0 54-55 0.0 3.40094525872521E-5 0.0 0.6218118263790238 0.0 56-57 0.0 3.40094525872521E-5 0.0 0.6831478741201329 0.0 58-59 0.0 3.40094525872521E-5 0.0 0.7254556331386746 0.0 60-61 0.0 3.40094525872521E-5 0.0 0.7612335772604637 0.0 62-63 1.020283577617563E-4 3.40094525872521E-5 0.0 0.7947668975114943 0.0 64-65 1.020283577617563E-4 5.101417888087815E-5 0.0 0.826888825480154 3.40094525872521E-5 66-67 1.020283577617563E-4 6.80189051745042E-5 0.0 0.854674548243939 3.40094525872521E-5 68-69 1.020283577617563E-4 8.502363146813024E-5 0.0 0.8934793336459936 3.40094525872521E-5 70-71 1.020283577617563E-4 1.020283577617563E-4 0.0 0.9556996271543713 3.40094525872521E-5 72-73 1.020283577617563E-4 1.020283577617563E-4 0.0 1.0433589911980135 3.40094525872521E-5 74-75 1.020283577617563E-4 1.020283577617563E-4 0.0 1.1547399484212642 3.40094525872521E-5 76-77 1.020283577617563E-4 1.020283577617563E-4 0.0 1.3372176662781654 3.40094525872521E-5 78-79 1.020283577617563E-4 1.020283577617563E-4 0.0 1.6071506914631852 3.40094525872521E-5 80-81 1.020283577617563E-4 1.020283577617563E-4 0.0 1.9371784193698798 3.40094525872521E-5 82-83 1.020283577617563E-4 1.020283577617563E-4 0.0 2.266644991308884 3.40094525872521E-5 84-85 1.020283577617563E-4 1.020283577617563E-4 0.0 2.5244196371939616 3.40094525872521E-5 86-87 1.020283577617563E-4 1.360378103490084E-4 0.0 2.7248373412906384 3.40094525872521E-5 88-89 1.020283577617563E-4 1.5304253664263445E-4 0.0 2.9183171170595155 3.40094525872521E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11110 0.0 33.175285 1 GTATCAA 16740 0.0 30.70677 1 GTACATG 13410 0.0 27.064037 1 TACATGG 13495 0.0 26.43471 2 ACATGGG 13320 0.0 25.649626 3 ATCAACG 20225 0.0 25.222574 3 TCAACGC 20255 0.0 25.161983 4 CAACGCA 20540 0.0 24.858673 5 TATCAAC 20705 0.0 24.707712 2 AACGCAG 20775 0.0 24.591381 6 CATGGGA 8615 0.0 22.505598 4 CATGGGG 4740 0.0 22.43772 4 ACGCAGA 23410 0.0 21.622639 7 CGCAGAG 23225 0.0 21.532164 8 GAGTACT 11645 0.0 20.785934 12-13 GCAGAGT 24065 0.0 20.057941 9 AGTACTT 12455 0.0 20.00018 12-13 AGAGTAC 19900 0.0 19.461483 10-11 ATGGGAT 3920 0.0 19.208017 5 GTACTTT 12810 0.0 18.91375 14-15 >>END_MODULE