FastQCFastQC Report
Fri 27 May 2016
SRR939704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986972
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61660.20642978909745385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.1474402840066797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31110.1041522987158902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140650.041.9057351
GGTATCA91250.037.6695751
GTACATG114600.030.7000661
TATCAAC193300.030.2544882
ATCAACG205850.028.4109253
TCAACGC208200.028.0642574
CAACGCA211350.027.6237245
AACGCAG216350.027.2262026
TACATGG137600.025.816682
ACGCAGA228500.025.509987
ACATGGG135100.025.3162163
CGCAGAG243200.023.7351678
GAGTACT110800.022.74773212-13
GCAGAGT242200.022.3315059
CATGGGA91300.021.8843754
AGTACTT122500.021.3649612-13
GTATAGG8850.020.5624051
GTACTTT123700.020.4741514-15
AGAGTAC196700.020.41925810-11
GAGTACA109950.019.6771971
Produced by FastQC (version 0.11.5)