FastQCFastQC Report
Fri 27 May 2016
SRR939704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986972
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155440.520393227656637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121250.4059294831019507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.2866448028304249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110950.032.9193081
GTATCAA170450.031.1453021
GTACATG136500.026.2389321
TACATGG136600.025.6644212
TCAACGC206850.025.383164
ATCAACG207200.025.294753
CAACGCA209950.025.0533085
TATCAAC212300.024.8920922
AACGCAG212250.024.8108986
ACATGGG137450.024.2993473
ACGCAGA238250.021.964797
GAGTACT117150.021.85216712-13
CGCAGAG238850.021.7911838
CATGGGA90950.021.4216754
AGTACTT125050.020.3397112-13
CATGGGG47150.020.310494
GCAGAGT249050.020.2172349
GTACTTT129900.019.48956514-15
AGAGTAC201550.019.43816410-11
ATGGGAT41300.019.3037975
Produced by FastQC (version 0.11.5)