##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939704_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2986972 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.477539461367567 31.0 31.0 34.0 30.0 34.0 2 31.681101463287906 31.0 31.0 34.0 30.0 34.0 3 31.728114960568764 31.0 31.0 34.0 30.0 34.0 4 35.53619551840459 37.0 35.0 37.0 33.0 37.0 5 35.2966261484875 37.0 35.0 37.0 33.0 37.0 6 35.299832740313605 37.0 35.0 37.0 32.0 37.0 7 35.20956105380298 37.0 35.0 37.0 32.0 37.0 8 35.17049607428526 37.0 35.0 37.0 32.0 37.0 9 36.76494255721178 39.0 35.0 39.0 32.0 39.0 10-11 36.64437915722009 39.0 35.0 39.0 32.0 39.0 12-13 36.57798901362316 39.0 35.0 39.0 32.0 39.0 14-15 37.63037199545225 39.0 36.0 41.0 32.0 41.0 16-17 37.587745884460915 39.0 36.0 41.0 32.0 41.0 18-19 37.58802777528547 39.0 36.0 41.0 32.0 41.0 20-21 37.54173758575574 39.0 36.0 41.0 32.0 41.0 22-23 37.402984862261846 39.0 36.0 41.0 32.0 41.0 24-25 37.27349285497152 39.0 36.0 41.0 31.0 41.0 26-27 36.98976153777136 39.0 36.0 40.0 31.0 41.0 28-29 36.79280923959113 39.0 36.0 40.0 30.0 41.0 30-31 36.53716389038799 38.0 35.0 40.0 30.0 41.0 32-33 36.337521744428805 38.0 35.0 40.0 30.0 41.0 34-35 36.29879138472005 38.0 35.0 40.0 30.0 41.0 36-37 36.19834333900686 38.0 35.0 40.0 30.0 41.0 38-39 36.228010339567966 38.0 35.0 40.0 30.0 41.0 40-41 36.11369055351038 38.0 35.0 40.0 30.0 41.0 42-43 35.91716912645984 38.0 35.0 40.0 29.0 41.0 44-45 35.674569764965995 38.0 35.0 40.0 28.0 41.0 46-47 35.347395288606656 38.0 34.0 40.0 27.0 41.0 48-49 35.01907399868496 38.0 34.0 40.0 26.0 41.0 50-51 34.776438145386024 38.0 34.0 40.0 25.5 41.0 52-53 34.54842847539247 38.0 33.0 40.0 24.0 41.0 54-55 34.27785914966729 38.0 33.0 40.0 24.0 41.0 56-57 33.51026691914086 37.5 32.5 40.0 20.5 41.0 58-59 30.52133481666383 35.5 27.0 39.0 8.0 40.5 60-61 30.255518130066168 34.5 26.0 39.0 10.5 40.0 62-63 32.004648018126716 35.5 30.5 39.0 17.0 40.0 64-65 32.050705865337875 36.0 31.0 39.0 12.5 40.0 66-67 27.2443467832976 32.0 18.5 37.0 2.0 39.5 68-69 19.812267573984624 20.0 8.0 32.5 2.0 37.0 70-71 17.869030911571986 15.5 7.5 29.0 2.0 35.0 72-73 19.583744340422342 20.0 7.5 31.0 2.0 36.0 74-75 21.016913449473243 23.5 9.0 32.5 2.0 36.0 76-77 22.83819332755714 27.5 11.5 33.0 2.0 36.0 78-79 24.989119918097657 30.5 13.0 34.5 2.0 37.0 80-81 26.571051051030942 32.0 22.5 35.0 2.0 37.0 82-83 27.667344722347583 33.0 26.5 35.0 2.0 36.5 84-85 27.69419616253517 34.0 27.0 35.0 2.0 36.0 86-87 27.41739678175758 33.5 26.5 35.0 2.0 36.0 88-89 26.97555367107559 33.0 26.0 35.0 2.0 35.5 90-91 25.170052313848274 32.0 16.0 34.5 2.0 35.0 92-93 23.583604734158875 31.0 2.0 34.0 2.0 35.0 94-95 19.875170741473305 28.0 2.0 34.0 2.0 35.0 96-97 17.185379541555797 18.5 2.0 33.0 2.0 35.0 98-99 17.453640007338535 21.5 2.0 33.0 2.0 35.0 100-101 16.67575357251424 10.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17346860702017608 1101 2 0.17134566978026555 1101 3 0.19591110398135214 1101 4 0.21600291430340235 1101 5 0.3451489022326726 1101 6 0.26921268046450564 1101 7 0.3019423523445397 1101 8 0.34905891599883176 1101 9 0.4086305028126418 1101 10-11 0.28104204289366663 1101 12-13 0.20411630854709273 1101 14-15 0.32170521979262645 1101 16-17 0.39670408487086206 1101 18-19 0.3713992920426179 1101 20-21 0.33984375341129436 1101 22-23 0.29776107628237014 1101 24-25 0.3551057024106754 1101 26-27 0.3765988719279889 1101 28-29 0.3184031608643636 1101 30-31 0.19731065588302954 1101 32-33 0.3246318607301504 1101 34-35 0.2144664531408651 1101 36-37 0.11789696924950732 1101 38-39 0.24238235299972644 1101 40-41 0.24618591832948056 1101 42-43 0.3010142935585449 1101 44-45 0.25138413674399374 1101 46-47 0.31371257948755016 1101 48-49 0.22467307165976536 1101 50-51 0.20748930764538187 1101 52-53 0.1977463527975658 1101 54-55 0.23210982138973435 1101 56-57 -3.1735906752148217 1101 58-59 -10.023994481292316 1101 60-61 -12.170789638856121 1101 62-63 -4.325365824397448 1101 64-65 -1.2914947363946183 1101 66-67 -2.0605022772294355 1101 68-69 -4.576740896226589 1101 70-71 -4.5365095024812945 1101 72-73 -3.546751664424036 1101 74-75 -6.36429891177977 1101 76-77 -4.952030352344099 1101 78-79 1.0802222176861669 1101 80-81 1.842438344815772 1101 82-83 0.5714174019941325 1101 84-85 0.20251189852570306 1101 86-87 0.1484017884425981 1101 88-89 -0.18774880176183117 1101 90-91 1.2814310627635699 1101 92-93 0.6956793392729601 1101 94-95 -8.500999834395046 1101 96-97 -9.751256681770718 1101 98-99 -8.894336404455476 1101 100-101 -9.555563122445072 1102 1 0.2174233801254708 1102 2 0.3309236718904849 1102 3 0.28705578304824897 1102 4 0.27216770496314524 1102 5 0.28928935625246055 1102 6 0.2288524226306592 1102 7 0.26246119918773303 1102 8 0.30892972262805785 1102 9 0.18609054455629348 1102 10-11 0.26969650130897804 1102 12-13 0.31333009040196913 1102 14-15 0.49756704646893724 1102 16-17 0.4345186020774463 1102 18-19 0.3035906263189503 1102 20-21 0.3171090144934041 1102 22-23 0.3699015671618966 1102 24-25 0.35725658312796327 1102 26-27 0.12156371695584056 1102 28-29 0.28459737020001796 1102 30-31 0.30527293455184434 1102 32-33 0.11748786817582157 1102 34-35 0.16357513867664863 1102 36-37 -0.13791039716850406 1102 38-39 0.11773102305441796 1102 40-41 0.05506093618341623 1102 42-43 0.08913048465783646 1102 44-45 0.056730756475928956 1102 46-47 -0.07311294495736576 1102 48-49 -0.12062224392867904 1102 50-51 0.010276940282828662 1102 52-53 0.025087406709531024 1102 54-55 -0.1942020396122075 1102 56-57 -3.4038086314346714 1102 58-59 -9.782620695935769 1102 60-61 -7.9745396561341195 1102 62-63 -2.497324665895981 1102 64-65 -0.9314338717573847 1102 66-67 -2.0520164751385686 1102 68-69 -5.065838017020548 1102 70-71 -4.618540381724195 1102 72-73 -3.5350424785173544 1102 74-75 -4.236331818308088 1102 76-77 -4.199970123537295 1102 78-79 1.7368555877256533 1102 80-81 1.9395681233348405 1102 82-83 0.5335440243983989 1102 84-85 0.2770705032004379 1102 86-87 0.058579567551422684 1102 88-89 -0.020764872606246598 1102 90-91 1.2477687946919325 1102 92-93 0.9334262419033656 1102 94-95 -8.771530476353403 1102 96-97 -9.681863968940306 1102 98-99 -8.787140232338944 1102 100-101 -9.492424469944831 1103 1 0.23289537616614808 1103 2 0.23718816965834577 1103 3 0.25890332730367405 1103 4 0.23020235427373592 1103 5 0.21618590978253138 1103 6 0.3002263931391127 1103 7 0.300824447839922 1103 8 0.3488138321361518 1103 9 0.27057649816954665 1103 10-11 0.3625692295101217 1103 12-13 0.3836823706130801 1103 14-15 0.4144991191211105 1103 16-17 0.4475021101368455 1103 18-19 0.4281745962993213 1103 20-21 0.5008032890561935 1103 22-23 0.471393579778308 1103 24-25 0.49037271453104125 1103 26-27 0.5600310440333445 1103 28-29 0.42790574609650633 1103 30-31 0.41915425132340545 1103 32-33 0.2811561836873935 1103 34-35 0.3738238139925514 1103 36-37 0.3427982155366749 1103 38-39 0.49139657376119317 1103 40-41 0.5056235750510254 1103 42-43 0.3587439051556274 1103 44-45 0.31297038845794134 1103 46-47 0.2821458687035161 1103 48-49 0.22481392966876257 1103 50-51 0.26061086629255925 1103 52-53 0.3208050470370125 1103 54-55 0.26981338219400897 1103 56-57 0.7613227175238464 1103 58-59 -8.051838435115386 1103 60-61 -7.509974873942692 1103 62-63 -1.8874762108043122 1103 64-65 -0.06531414327125162 1103 66-67 -1.5389329693049838 1103 68-69 -3.2895957206133986 1103 70-71 -3.7991318884708 1103 72-73 -2.8567806525940043 1103 74-75 -3.6366024505820675 1103 76-77 -0.34732232649971806 1103 78-79 3.5169909780563025 1103 80-81 3.0620477757244657 1103 82-83 1.4467926547616763 1103 84-85 1.0066495174809695 1103 86-87 0.9395272802264465 1103 88-89 0.773060065714823 1103 90-91 2.2056145229484976 1103 92-93 1.8628329414159985 1103 94-95 -7.081948335028454 1103 96-97 -8.630834935653102 1103 98-99 -7.852502880022509 1103 100-101 -9.323039042190032 1104 1 0.2001991037766544 1104 2 0.23803022503917504 1104 3 0.1617664219454653 1104 4 0.12557989774784772 1104 5 0.15846789475315148 1104 6 0.1297142197067629 1104 7 0.21906308237873873 1104 8 0.24294977462880496 1104 9 0.3315390531854945 1104 10-11 0.20282686782871906 1104 12-13 0.2037321154760363 1104 14-15 0.30007274728230726 1104 16-17 0.310533688170743 1104 18-19 0.28368606039732924 1104 20-21 0.20075182775298828 1104 22-23 0.18036842467655845 1104 24-25 0.28477816153823454 1104 26-27 0.236583513186865 1104 28-29 0.2658111487570096 1104 30-31 0.18204712099242215 1104 32-33 0.23513362451540587 1104 34-35 0.16680293531552337 1104 36-37 0.06842891161381459 1104 38-39 0.09319721954258853 1104 40-41 0.017319456296327473 1104 42-43 0.12465979405366312 1104 44-45 0.18670228049192872 1104 46-47 0.09342220048142025 1104 48-49 0.018201742432566448 1104 50-51 0.347630185922867 1104 52-53 0.18113275312629185 1104 54-55 0.15277810626294297 1104 56-57 0.5103057745358228 1104 58-59 -8.192301471989499 1104 60-61 -7.3383736010969685 1104 62-63 -2.1177009368598085 1104 64-65 -0.41908736599522456 1104 66-67 -1.6823263971892892 1104 68-69 -3.1604430040117215 1104 70-71 -3.768244950410896 1104 72-73 -2.8285905144046133 1104 74-75 -3.531001655021587 1104 76-77 -0.23998339591218 1104 78-79 3.542974575949014 1104 80-81 2.9289916996004486 1104 82-83 1.3052250449887275 1104 84-85 0.9814263004891082 1104 86-87 0.9284842630860801 1104 88-89 0.8446435467109374 1104 90-91 2.3088075697277723 1104 92-93 2.0109929472523227 1104 94-95 -6.135884385612181 1104 96-97 -7.714846922233816 1104 98-99 -5.473530474723962 1104 100-101 -4.865531147522036 1105 1 0.24669454155985449 1105 2 0.26293150372811525 1105 3 0.2184894313585879 1105 4 0.13945480229099871 1105 5 0.24553841194205717 1105 6 0.27393974004436217 1105 7 0.23239596098182602 1105 8 0.2577075442061485 1105 9 0.2628328587791344 1105 10-11 0.2288288335983779 1105 12-13 0.24250381398321252 1105 14-15 0.33907243668902964 1105 16-17 0.35553706840178734 1105 18-19 0.3450790243019668 1105 20-21 0.27552548994390236 1105 22-23 0.38700134326670366 1105 24-25 0.3442259020816678 1105 26-27 0.37261679693791905 1105 28-29 0.3475142845809671 1105 30-31 0.36096996724499775 1105 32-33 0.3072343906524395 1105 34-35 0.3082002629618117 1105 36-37 0.38810703977723904 1105 38-39 0.3872601112389873 1105 40-41 0.28556988792502835 1105 42-43 0.32282211022426566 1105 44-45 0.28299871121409126 1105 46-47 0.16676153291655993 1105 48-49 0.16856476527066633 1105 50-51 0.1449039842199582 1105 52-53 0.14751567229006213 1105 54-55 0.15000268731141375 1105 56-57 0.8575001410538263 1105 58-59 -8.371457941121765 1105 60-61 -7.314767364951379 1105 62-63 -1.8941814123491199 1105 64-65 -0.032986029150869456 1105 66-67 -1.3778014244678864 1105 68-69 -3.132230903222638 1105 70-71 -3.4567866972550583 1105 72-73 -2.6179307821656366 1105 74-75 -3.625723269372372 1105 76-77 -0.13962938424229776 1105 78-79 3.8946974195316137 1105 80-81 3.2882303163777813 1105 82-83 1.7420075281120795 1105 84-85 1.3626363147338445 1105 86-87 1.2788894319088548 1105 88-89 1.220942369981696 1105 90-91 2.6285825366607476 1105 92-93 2.2885102807141458 1105 94-95 -5.870076356035524 1105 96-97 -7.60819411441012 1105 98-99 -5.280712073334406 1105 100-101 -4.614591806242602 1106 1 0.17448773339206625 1106 2 0.19383283574582677 1106 3 0.23468579635535392 1106 4 0.19057440672848713 1106 5 0.32591673143955546 1106 6 0.21943545265997955 1106 7 0.21287740106598108 1106 8 0.21605472257510172 1106 9 0.2310371911528506 1106 10-11 0.22832325329476788 1106 12-13 0.27683648531162675 1106 14-15 0.2761280212727044 1106 16-17 0.25856638776398455 1106 18-19 0.23126638836365743 1106 20-21 0.26323191648472744 1106 22-23 0.2281860774108253 1106 24-25 0.237074530817182 1106 26-27 0.4025371078276194 1106 28-29 0.4248990593678812 1106 30-31 0.4171548859895893 1106 32-33 0.42055535524259113 1106 34-35 0.25263255411552166 1106 36-37 0.3902896207946043 1106 38-39 0.4005645384901868 1106 40-41 0.2651844728148163 1106 42-43 0.24944679639067147 1106 44-45 0.3344665508966074 1106 46-47 0.36239625838292255 1106 48-49 0.28047632313197113 1106 50-51 0.12066655548674277 1106 52-53 0.26496933526973265 1106 54-55 0.2599435200467113 1106 56-57 0.9240925379892317 1106 58-59 -7.94620124163303 1106 60-61 -7.467771397628017 1106 62-63 -1.9383146302790308 1106 64-65 -0.18943815324779933 1106 66-67 1.0244660216320973 1106 68-69 -2.1210852788112753 1106 70-71 -2.175633628484375 1106 72-73 -0.7944850798129863 1106 74-75 -1.0159865198768543 1106 76-77 2.3110306928521815 1106 78-79 4.52269739457731 1106 80-81 3.534102910550356 1106 82-83 1.9352858106178985 1106 84-85 1.4683935688933119 1106 86-87 1.2828485240345167 1106 88-89 1.2671489938558231 1106 90-91 2.5589421353178388 1106 92-93 2.3077579739496805 1106 94-95 -6.48757829127236 1106 96-97 -7.950794238179368 1106 98-99 -5.801705391615386 1106 100-101 -5.081846443319604 1107 1 1.1547718441917709E-4 1107 2 0.09261771320025503 1107 3 0.030576975298270526 1107 4 0.024337728921118185 1107 5 0.014969959444421477 1107 6 0.036811702053448414 1107 7 0.07836681174844529 1107 8 0.20573859979278097 1107 9 0.14409768536612688 1107 10-11 -0.02624838444688038 1107 12-13 0.008195493102768125 1107 14-15 0.07870442480638218 1107 16-17 0.06950759458790401 1107 18-19 0.14984567736340182 1107 20-21 0.14156709601765272 1107 22-23 0.11150599584046006 1107 24-25 0.14702334227208524 1107 26-27 0.1899471162515809 1107 28-29 0.18862149309445897 1107 30-31 0.17836015768074986 1107 32-33 0.05690350393072663 1107 34-35 0.1599153477392221 1107 36-37 0.20073813448778566 1107 38-39 0.14882823013999769 1107 40-41 0.2234005657056457 1107 42-43 0.1161825547415134 1107 44-45 0.15805122507688907 1107 46-47 0.14386873048913884 1107 48-49 0.12883262725263478 1107 50-51 0.19944571535445732 1107 52-53 0.1592723447037514 1107 54-55 0.0828809798559007 1107 56-57 0.5629109709192406 1107 58-59 -8.559764827323985 1107 60-61 -7.926444837291886 1107 62-63 -2.430642013959684 1107 64-65 -0.4625991674887189 1107 66-67 0.5610995382617823 1107 68-69 -2.6803428709331563 1107 70-71 -2.367388120404799 1107 72-73 -0.8915030103935422 1107 74-75 -0.888487595057903 1107 76-77 2.24447405279572 1107 78-79 4.2167097416366275 1107 80-81 3.0626769061196804 1107 82-83 1.4525571439998295 1107 84-85 1.0647960717563656 1107 86-87 0.9250887350738317 1107 88-89 0.7626920939008563 1107 90-91 2.1669871769057103 1107 92-93 1.7501763005498248 1107 94-95 -6.83512453709309 1107 96-97 -8.156966133382884 1107 98-99 -5.981615043316889 1107 100-101 -5.438999327797982 1108 1 -0.057722998633813916 1108 2 -0.07425901085915143 1108 3 -0.0873814948110514 1108 4 -0.06101906095637588 1108 5 -0.06394764665144947 1108 6 -0.06479736322931018 1108 7 -0.09016013825620917 1108 8 2.74801934992297E-4 1108 9 -0.16359477969967173 1108 10-11 -0.1839130599766463 1108 12-13 -0.06844536818287139 1108 14-15 -0.17168348399328437 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98-99 -0.5422416278364786 2312 100-101 0.06330095753492415 2313 1 -0.29684073533879385 2313 2 -0.2767479906718684 2313 3 -0.20064592392910896 2313 4 -0.15175012786718156 2313 5 -0.1447492670725694 2313 6 -0.19657480654586124 2313 7 -0.25797034116452267 2313 8 -0.3275183107349733 2313 9 -0.3239596535871314 2313 10-11 -0.3364809403233622 2313 12-13 -0.4374113232215038 2313 14-15 -0.3310015132492836 2313 16-17 -0.30271363667363715 2313 18-19 -0.40021138269282375 2313 20-21 -0.34160659064401244 2313 22-23 -0.3334268040204478 2313 24-25 -0.228042491840732 2313 26-27 -0.48171313538551175 2313 28-29 -0.4457245755268815 2313 30-31 -0.41663232779764314 2313 32-33 -0.3955580928750422 2313 34-35 -0.2728611034364974 2313 36-37 -0.34380680754744475 2313 38-39 -0.508360588751259 2313 40-41 -0.42112776919552886 2313 42-43 -0.2620179792021773 2313 44-45 -0.1946827874811845 2313 46-47 -0.42420899787524746 2313 48-49 -0.50025947293841 2313 50-51 -0.37991389372701434 2313 52-53 -0.17695900543421317 2313 54-55 -0.31547775976086 2313 56-57 0.47702541913505314 2313 58-59 3.469491498970495 2313 60-61 3.454138118096804 2313 62-63 0.7289356984769135 2313 64-65 0.0373356065922863 2313 66-67 -1.9996230841341287 2313 68-69 -4.894675516142993 2313 70-71 -3.3808159843482137 2313 72-73 -2.809564108673168 2313 74-75 -3.524038897285802 2313 76-77 0.540777042199192 2313 78-79 3.053713429081366 2313 80-81 2.4548518522245253 2313 82-83 1.073236707190894 2313 84-85 0.6573603720732066 2313 86-87 0.6590032477168712 2313 88-89 0.7282995363205096 2313 90-91 2.4101393963908784 2313 92-93 3.680610402220921 2313 94-95 5.739855158413409 2313 96-97 1.0653413787793866 2313 98-99 2.6076308137815225 2313 100-101 3.6329669394314603 2314 1 -0.3660591173283372 2314 2 -0.299451985916523 2314 3 -0.3630932200226482 2314 4 -0.21528559871129715 2314 5 -0.20808907869349724 2314 6 -0.28150773576482635 2314 7 -0.25241755946787237 2314 8 -0.4281192821821733 2314 9 -0.3618939521669944 2314 10-11 -0.3340497856929687 2314 12-13 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0.4645417319664453 2314 78-79 3.338116412673134 2314 80-81 2.407657985134975 2314 82-83 0.841093126804676 2314 84-85 0.49349577265249067 2314 86-87 0.5237107714227953 2314 88-89 0.5553850213909222 2314 90-91 2.1159206444751923 2314 92-93 3.752901581025462 2314 94-95 6.398364622392535 2314 96-97 3.750953281015729 2314 98-99 5.910634191312926 2314 100-101 6.30600722925063 2315 1 -0.4162377812110698 2315 2 -0.4303591401106672 2315 3 -0.44796068622079943 2315 4 -0.2715494441012609 2315 5 -0.391419016988678 2315 6 -0.4182921253794021 2315 7 -0.5404674495535176 2315 8 -0.6601349163328578 2315 9 -0.7054926077318839 2315 10-11 -0.5486246844783906 2315 12-13 -0.6060164339203808 2315 14-15 -0.7937427860350965 2315 16-17 -0.6786338418374882 2315 18-19 -0.6540150222899186 2315 20-21 -0.817423694331481 2315 22-23 -0.7799286786701742 2315 24-25 -0.7176026688635346 2315 26-27 -0.8151462058760899 2315 28-29 -0.8235786679868795 2315 30-31 -0.8362973693704134 2315 32-33 -0.7568934635378426 2315 34-35 -0.7112134473022778 2315 36-37 -0.6284070838474776 2315 38-39 -0.8324942126936321 2315 40-41 -0.7462653655896574 2315 42-43 -0.8364035012167435 2315 44-45 -0.8160610766354779 2315 46-47 -0.7575494857100509 2315 48-49 -0.7944830221503167 2315 50-51 -0.7623289808671316 2315 52-53 -0.6562080979197731 2315 54-55 -0.7783360597491011 2315 56-57 -0.13711179057531098 2315 58-59 2.9855393012609532 2315 60-61 3.180978911701356 2315 62-63 0.5239234165827433 2315 64-65 -0.2480195547843067 2315 66-67 -1.9873325449402337 2315 68-69 -4.920727014925875 2315 70-71 -3.7690015601918923 2315 72-73 -2.8589645784854305 2315 74-75 -0.526171875619589 2315 76-77 1.8601532207755511 2315 78-79 3.5646664597792928 2315 80-81 2.5353727425170582 2315 82-83 0.8080698028925717 2315 84-85 0.4968748965628933 2315 86-87 0.44905133618739157 2315 88-89 0.47821368230299655 2315 90-91 2.1306580807121307 2315 92-93 3.5865421653115312 2315 94-95 6.153028980433259 2315 96-97 4.021892815386245 2315 98-99 6.305669160205891 2315 100-101 6.519745463321533 2316 1 -0.43345568936944545 2316 2 -0.39355058867743864 2316 3 -0.4641815662898239 2316 4 -0.35419252721888483 2316 5 -0.4778201290584647 2316 6 -0.5290005905313535 2316 7 -0.4626081839030505 2316 8 -0.44047716539814274 2316 9 -0.6739280405762926 2316 10-11 -0.586300842085528 2316 12-13 -0.5264315427306556 2316 14-15 -0.6269395073465702 2316 16-17 -0.7282911134317729 2316 18-19 -0.6831061928392472 2316 20-21 -0.6101856538751491 2316 22-23 -0.8746846943925419 2316 24-25 -0.6915385331143256 2316 26-27 -0.6808014642405809 2316 28-29 -0.5681669145595976 2316 30-31 -0.6097874470235638 2316 32-33 -0.4753960040421106 2316 34-35 -0.42409092788899017 2316 36-37 -0.4989659947860261 2316 38-39 -0.6188641676356355 2316 40-41 -0.605842587332539 2316 42-43 -0.6467764280693942 2316 44-45 -0.5906584306763136 2316 46-47 -0.7114465233862006 2316 48-49 -0.8048487588965543 2316 50-51 -0.6560400344573978 2316 52-53 -0.6142634138051193 2316 54-55 -0.41788883513017794 2316 56-57 0.34646121297181054 2316 58-59 3.371288510349242 2316 60-61 3.401561789661283 2316 62-63 0.7924906680447314 2316 64-65 0.23632998025547636 2316 66-67 -1.8062640951271796 2316 68-69 -2.942445929699147 2316 70-71 -3.062202116226789 2316 72-73 -0.6097171429567183 2316 74-75 5.713742801784306 2316 76-77 5.939833980050789 2316 78-79 4.9521844350453215 2316 80-81 3.2363769607128248 2316 82-83 1.5307114479889172 2316 84-85 1.063443298441907 2316 86-87 0.8288902778037297 2316 88-89 0.8076212175345212 2316 90-91 2.4653671210725925 2316 92-93 3.9546947873036693 2316 94-95 6.64487060635599 2316 96-97 4.030710282551425 2316 98-99 6.336402071719647 2316 100-101 6.514652471278586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 18.0 4 46.0 5 144.0 6 298.0 7 751.0 8 1637.0 9 2791.0 10 4753.0 11 7588.0 12 10904.0 13 15128.0 14 18497.0 15 24335.0 16 32689.0 17 42806.0 18 44891.0 19 42323.0 20 44096.0 21 45195.0 22 45457.0 23 45977.0 24 49179.0 25 52067.0 26 64919.0 27 90702.0 28 127494.0 29 169347.0 30 206129.0 31 231119.0 32 240951.0 33 242970.0 34 247765.0 35 265984.0 36 268441.0 37 208841.0 38 84965.0 39 5773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02204553970786 20.391538215025886 14.574153133353848 23.012263111912407 2 15.159021462021084 21.90450073629418 40.015462512614775 22.921015289069967 3 18.410286558333773 26.739641495751876 30.95883916991355 23.8912327760008 4 12.351103391662193 17.8121857185136 38.350911893382325 31.485798996441883 5 12.44624999497507 38.92706347090029 34.946347143628586 13.68033939049605 6 29.98943411588726 38.17434512275308 17.984868957593175 13.851351803766491 7 26.52843749455971 34.05609426536305 22.155848799386135 17.259619440691107 8 25.279062059545293 35.91673167122536 20.555948000816883 18.24825826841247 9 26.467841010896652 16.251173429144966 20.912415650364316 36.368569909594065 10-11 24.00961575803188 27.382831174848643 28.33749362230379 20.270059444815686 12-13 25.212958139547343 24.98329411859234 28.707081954568036 21.09666578729228 14-15 22.188899574232305 25.933981317109467 27.339090027226703 24.538029081431525 16-17 21.038831298050333 29.21274454531211 27.909987103996958 21.8384370526406 18-19 21.004540909785597 27.791650129487994 30.409197455671016 20.794611505055396 20-21 21.963731163198048 26.918012622816683 30.320588877297812 20.797667336687457 22-23 21.571410779880093 27.055359072666228 30.082555176278852 21.290674971174823 24-25 21.445511672363633 27.071212633538334 30.307150724647386 21.176124969450647 26-27 21.306247235910384 27.143552544402034 30.322226310350057 21.227973909337525 28-29 21.105313473350442 27.352265835538624 30.42701329067212 21.11540740043881 30-31 21.335660891630265 27.18827140963586 30.279919687185807 21.196148011548065 32-33 21.199157628301815 27.252917415097404 30.14224229569724 21.40568266090354 34-35 21.527909370126665 27.27175637228742 30.049669927329774 21.150664330256134 36-37 21.556316220821962 27.019402101911126 30.093277918541062 21.331003758725853 38-39 21.334781382152883 27.39616565249643 30.122112529676286 21.146940435674402 40-41 21.419241264916984 27.23639786708893 30.14915601448053 21.19520485351356 42-43 21.26287569520754 27.36058111870175 29.830935376545863 21.54560780954484 44-45 21.38614551451868 27.44715620960192 29.63422332813512 21.532474947744277 46-47 21.550977752372425 27.547346320888806 29.261018100866544 21.64065782587222 48-49 21.55408107181332 27.65703997304988 29.157321317681223 21.631557637455572 50-51 21.484983394914924 27.793927426749516 29.073941122217644 21.647148056117917 52-53 21.672532984955257 27.93596877996491 28.826359490999536 21.565138744080294 54-55 21.428002025734326 27.801394969962324 28.933719500104505 21.83688350419884 56-57 21.436554282473285 28.09555141108668 28.772292107556225 21.695602198883808 58-59 20.73352673929251 27.449487147201417 30.234556557758246 21.582429555747826 60-61 21.060436838171697 27.833544670523917 29.727215471561852 21.37880301974253 62-63 21.553778524748232 27.827298050139277 29.048237625883864 21.570685799228624 64-65 21.38514994634102 27.77243503630916 29.007369970087904 21.835045047261918 66-67 21.547018542488047 27.643464118592593 29.11258786474477 21.69692947417459 68-69 21.15589933805341 27.06814180251144 30.3105764415838 21.46538241785134 70-71 21.976659446460783 27.116913702118445 29.295158600923898 21.611268250496877 72-73 21.16794652721552 27.335459343004732 29.667787272469788 21.828806857309964 74-75 21.2042661476673 27.591213404017612 29.279718845203806 21.924801603111284 76-77 19.943904869295444 27.870255095751755 29.84235322327788 22.34348681167492 78-79 21.181896580892857 27.799050883019504 28.856913486123737 22.1621390499639 80-81 21.01022218940029 28.08944378047018 28.53197048638585 22.36836354374368 82-83 21.396388780733915 28.053957038054296 28.323946448724442 22.225707732487347 84-85 21.315820838357997 27.84179362516882 28.221530840654662 22.620854695818522 86-87 21.10924037207359 27.937977244909163 28.47124946768193 22.481532915335315 88-89 21.32033711732149 27.774481983761483 28.28811250992644 22.617068388990592 90-91 21.103435558217328 27.7669358633771 28.557697701376057 22.571930877029516 92-93 20.902805180918794 27.837422644980574 28.627520800519353 22.63225137358128 94-95 20.07490225365725 27.68982390451441 29.15524180603476 23.08003203579358 96-97 19.96687137506766 27.715528779174097 28.98184613295456 23.335753712803683 98-99 20.421161793222577 27.458836542399272 28.738756813969186 23.38124485040896 100-101 19.592684973870337 27.686837268914168 29.284125022429798 23.436352734785697 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1026.0 1 792.5 2 606.5 3 734.5 4 819.0 5 882.5 6 1153.5 7 1794.5 8 2587.5 9 3203.5 10 3496.5 11 3689.5 12 3956.5 13 4159.0 14 4473.5 15 4924.5 16 5325.5 17 5822.5 18 6384.0 19 6919.0 20 7642.0 21 8727.5 22 10177.0 23 11991.0 24 14182.5 25 17021.0 26 20965.5 27 25758.5 28 30422.5 29 35382.0 30 41393.5 31 47899.0 32 55088.5 33 62895.5 34 71177.5 35 79721.5 36 88046.5 37 94504.0 38 97961.0 39 99733.5 40 100691.5 41 101723.5 42 103252.0 43 105625.5 44 108307.5 45 110026.0 46 111371.5 47 112957.0 48 115110.5 49 113079.5 50 106343.5 51 100642.5 52 95120.0 53 89573.0 54 83871.5 55 76501.0 56 68676.0 57 60611.5 58 52566.5 59 45063.5 60 37189.5 61 29252.0 62 22405.5 63 16699.5 64 12126.5 65 8604.0 66 5831.5 67 3949.0 68 2632.5 69 1619.5 70 993.5 71 589.5 72 364.5 73 268.0 74 181.0 75 109.0 76 59.5 77 24.0 78 11.0 79 5.0 80 2.5 81 4.5 82 4.5 83 2.0 84 0.0 85 0.0 86 1.0 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02340162545882586 2 0.013190615780797409 3 2.0087232153498592E-4 4 0.0 5 0.062136504794822314 6 0.0 7 0.0 8 6.695744051166197E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.8544144370954933E-4 16-17 0.0 18-19 5.189201639653803E-4 20-21 0.0 22-23 0.0 24-25 6.695744051166197E-5 26-27 1.0043616076749296E-4 28-29 9.206648070353522E-4 30-31 0.00505528675863048 32-33 0.005590946282723775 34-35 0.0018078508938148734 36-37 0.08202286462678592 38-39 0.07586278009971302 40-41 0.08076741261719227 42-43 0.08441659312507784 44-45 0.08737945986771888 46-47 0.08282635391292587 48-49 0.07360296648244444 50-51 0.06839702548266272 52-53 0.057231872277343075 54-55 0.04646846371509341 56-57 0.06705787667242948 58-59 0.6920888444886661 60-61 0.3313388943719593 62-63 0.0030800422635364508 64-65 0.001791111533686958 66-67 1.0786006698422348 68-69 2.3222514305457165 70-71 2.3101488731732336 72-73 1.7741545618773793 74-75 1.262382104686619 76-77 0.7308572025449184 78-79 0.5603500802819712 80-81 0.03895249101765936 82-83 1.0043616076749296E-4 84-85 0.0012721913697215776 86-87 0.0012052339292099156 88-89 0.0 90-91 0.32953104347814444 92-93 0.057248611637470984 94-95 0.9301058061474965 96-97 1.6313343412660046 98-99 0.6326306373143102 100-101 0.5555124052050036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2986972.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.198035797010334 #Duplication Level Percentage of deduplicated Percentage of total 1 70.7434076182176 33.38949885167128 2 14.736419517294763 13.910601117940798 3 5.691147135880479 8.05832898736019 4 2.810837594094277 5.306640535425764 5 1.6099870518616537 3.7994113253244732 6 0.999585209054068 2.830707508745758 7 0.6677313027791293 2.206092415194758 8 0.4805831640201035 1.8146065103089069 9 0.34959925713784845 1.4850358427700354 >10 1.7180766501348348 14.319791830404027 >50 0.11800058988616083 3.825802293727746 >100 0.06988103036744375 6.029204629060688 >500 0.003540208411833773 1.0656053994603203 >1k 9.912583552085259E-4 0.7478987937981497 >5k 7.080416822918042E-5 0.28612642861929216 >10k+ 1.4160833645836084E-4 0.9246475301877124 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15544 0.520393227656637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12125 0.4059294831019507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8562 0.2866448028304249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.347872025583099E-5 0.0 14-15 0.0 0.0 0.0 3.347872025583099E-5 0.0 16-17 0.0 0.0 0.0 1.1717552089540847E-4 0.0 18-19 0.0 0.0 0.0 2.1761168166290143E-4 0.0 20-21 0.0 0.0 0.0 2.845691221745634E-4 0.0 22-23 0.0 0.0 0.0 5.356595240932958E-4 0.0 24-25 0.0 0.0 0.0 0.0010211009678028453 0.0 26-27 0.0 0.0 0.0 0.0014730636912565634 0.0 28-29 0.0 0.0 0.0 0.0033143933053272675 0.0 30-31 0.0 0.0 0.0 0.009189908710225608 0.0 32-33 1.6739360127915495E-5 0.0 0.0 0.021828125606801807 0.0 34-35 3.347872025583099E-5 0.0 0.0 0.0411620865545442 0.0 36-37 3.347872025583099E-5 0.0 0.0 0.06930095092957014 0.0 38-39 3.347872025583099E-5 0.0 0.0 0.11381090950969745 0.0 40-41 3.347872025583099E-5 0.0 0.0 0.16503335150111886 0.0 42-43 3.347872025583099E-5 0.0 0.0 0.21695884661791273 0.0 44-45 3.347872025583099E-5 0.0 0.0 0.2792292662937583 0.0 46-47 3.347872025583099E-5 0.0 0.0 0.35040502555765507 0.0 48-49 3.347872025583099E-5 0.0 0.0 0.4254643163712281 0.0 50-51 3.347872025583099E-5 0.0 0.0 0.5039551760110239 0.0 52-53 3.347872025583099E-5 0.0 0.0 0.5811738442810981 0.0 54-55 3.347872025583099E-5 0.0 0.0 0.6642847673161985 0.0 56-57 3.347872025583099E-5 1.6739360127915495E-5 0.0 0.7471111212291244 0.0 58-59 3.347872025583099E-5 6.695744051166198E-5 0.0 0.8131981150141347 0.0 60-61 3.347872025583099E-5 6.695744051166198E-5 0.0 0.8679358226324183 0.0 62-63 1.1717552089540847E-4 6.695744051166198E-5 0.0 0.9162288766014546 0.0 64-65 1.6739360127915494E-4 1.3391488102332396E-4 1.6739360127915495E-5 0.9710502810203778 0.0 66-67 1.6739360127915494E-4 1.3391488102332396E-4 3.347872025583099E-5 1.019745079632484 0.0 68-69 1.6739360127915494E-4 1.3391488102332396E-4 3.347872025583099E-5 1.07639107430535 0.0 70-71 1.6739360127915494E-4 1.3391488102332396E-4 6.695744051166198E-5 1.16592991162957 0.0 72-73 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 1.2865035226309454 0.0 74-75 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 1.4501307678813193 3.347872025583099E-5 76-77 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 1.6538487806380506 3.347872025583099E-5 78-79 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 1.9150162773537884 3.347872025583099E-5 80-81 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 2.23236106665881 3.347872025583099E-5 82-83 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 2.549036281558716 3.347872025583099E-5 84-85 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 2.829320127540533 5.0218080383746486E-5 86-87 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 3.089717613690386 6.695744051166198E-5 88-89 1.6739360127915494E-4 1.3391488102332396E-4 1.0043616076749297E-4 3.3447752439594343 6.695744051166198E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11095 0.0 32.919308 1 GTATCAA 17045 0.0 31.145302 1 GTACATG 13650 0.0 26.238932 1 TACATGG 13660 0.0 25.664421 2 TCAACGC 20685 0.0 25.38316 4 ATCAACG 20720 0.0 25.29475 3 CAACGCA 20995 0.0 25.053308 5 TATCAAC 21230 0.0 24.892092 2 AACGCAG 21225 0.0 24.810898 6 ACATGGG 13745 0.0 24.299347 3 ACGCAGA 23825 0.0 21.96479 7 GAGTACT 11715 0.0 21.852167 12-13 CGCAGAG 23885 0.0 21.791183 8 CATGGGA 9095 0.0 21.421675 4 AGTACTT 12505 0.0 20.33971 12-13 CATGGGG 4715 0.0 20.31049 4 GCAGAGT 24905 0.0 20.217234 9 GTACTTT 12990 0.0 19.489565 14-15 AGAGTAC 20155 0.0 19.438164 10-11 ATGGGAT 4130 0.0 19.303797 5 >>END_MODULE