##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939703_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 746266 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51002055567318 31.0 31.0 34.0 30.0 34.0 2 31.70718081756371 31.0 31.0 34.0 30.0 34.0 3 31.74486979173646 31.0 31.0 34.0 30.0 34.0 4 35.52882618262121 37.0 35.0 37.0 33.0 37.0 5 35.25252925900416 37.0 35.0 37.0 33.0 37.0 6 35.283199288189465 37.0 35.0 37.0 32.0 37.0 7 35.21441148330488 37.0 35.0 37.0 32.0 37.0 8 35.18952089469438 37.0 35.0 37.0 32.0 37.0 9 36.775688561451275 39.0 35.0 39.0 32.0 39.0 10-11 36.65161282974167 39.0 35.0 39.0 32.0 39.0 12-13 36.60748647265184 39.0 35.0 39.0 32.0 39.0 14-15 37.612670281106205 39.0 36.0 41.0 32.0 41.0 16-17 37.57539268839797 39.0 36.0 41.0 32.0 41.0 18-19 37.56916970624415 39.0 36.0 41.0 32.0 41.0 20-21 37.48160642451887 39.0 36.0 41.0 32.0 41.0 22-23 37.33880278613792 39.0 36.0 41.0 31.5 41.0 24-25 37.1640500840183 39.0 36.0 40.5 31.0 41.0 26-27 36.861818038072215 39.0 36.0 40.0 30.5 41.0 28-29 36.68189559754833 38.5 35.0 40.0 30.0 41.0 30-31 36.43705126590251 38.0 35.0 40.0 30.0 41.0 32-33 36.202356800390206 38.0 35.0 40.0 30.0 41.0 34-35 36.135006820624284 38.0 35.0 40.0 30.0 41.0 36-37 36.02279415114718 38.0 35.0 40.0 30.0 41.0 38-39 36.02612406300166 38.0 35.0 40.0 30.0 41.0 40-41 35.95037627333953 38.0 35.0 40.0 29.0 41.0 42-43 35.716766541688884 38.0 35.0 40.0 28.5 41.0 44-45 35.46433912304728 38.0 34.0 40.0 27.5 41.0 46-47 35.11374429492969 38.0 34.0 40.0 26.5 41.0 48-49 34.735463628250514 38.0 33.0 40.0 26.0 41.0 50-51 34.41655924295091 38.0 33.0 40.0 24.0 41.0 52-53 34.171462990408244 37.5 33.0 40.0 24.0 41.0 54-55 33.85260483527321 37.0 33.0 40.0 23.5 41.0 56-57 31.754761706951676 36.0 29.5 39.5 14.5 40.5 58-59 26.714036281969165 32.0 16.5 37.5 4.0 40.0 60-61 27.344807347514156 32.0 18.0 37.0 6.5 40.0 62-63 30.312778553491647 34.0 29.0 37.5 2.0 40.0 64-65 30.47852709355645 35.0 29.5 38.0 2.0 40.0 66-67 23.91667314335639 26.5 16.5 34.5 2.0 37.5 68-69 16.71595851881233 13.0 7.0 28.0 2.0 34.5 70-71 15.57934302246116 12.0 7.0 25.0 2.0 32.5 72-73 16.649650392755397 13.0 7.0 28.0 2.0 34.0 74-75 17.609219768822378 16.5 7.5 29.0 2.0 34.0 76-77 18.762482144436433 20.5 7.0 30.0 2.0 33.5 78-79 21.76177127190573 26.5 9.0 33.0 2.0 35.0 80-81 25.22830532276695 30.5 21.0 34.0 2.0 35.0 82-83 26.515037533533622 32.5 25.0 35.0 2.0 35.0 84-85 26.59190087716712 33.0 25.0 35.0 2.0 35.0 86-87 26.436971535618667 33.0 25.0 35.0 2.0 35.0 88-89 26.034869604135793 33.0 24.5 35.0 2.0 35.0 90-91 24.734036523170023 32.5 11.0 34.5 2.0 35.0 92-93 22.316294726009225 31.0 2.0 34.0 2.0 35.0 94-95 15.326158501124263 4.5 2.0 32.0 2.0 34.5 96-97 12.83787684284156 2.0 2.0 29.0 2.0 34.0 98-99 12.53447698273806 2.0 2.0 29.5 2.0 34.0 100-101 12.24060187654268 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4027350835819199 1101 2 0.460806439304136 1101 3 0.4405380181314378 1101 4 0.3782635925762676 1101 5 0.3913308659683281 1101 6 0.4939195649687207 1101 7 0.508471773910756 1101 8 0.6247066687119585 1101 9 0.6556695615641033 1101 10-11 0.5269175291363482 1101 12-13 0.5408453129230324 1101 14-15 0.7657123491137909 1101 16-17 0.850754189326203 1101 18-19 0.7725536611072528 1101 20-21 0.7301216257717371 1101 22-23 0.8759978966945638 1101 24-25 0.6424647991073016 1101 26-27 0.767556114880442 1101 28-29 0.8315508989367828 1101 30-31 0.9520817847056193 1101 32-33 0.9687637736644206 1101 34-35 0.759203314724104 1101 36-37 0.3387874489156175 1101 38-39 0.5569244614810103 1101 40-41 0.38833659334786574 1101 42-43 0.5978441946046757 1101 44-45 0.4059009807844447 1101 46-47 0.7748250179674088 1101 48-49 0.6473939192211304 1101 50-51 0.5859913268651553 1101 52-53 0.6846558595883749 1101 54-55 0.37811769369341164 1101 56-57 -2.12752176657623 1101 58-59 -10.300046437138521 1101 60-61 -10.637998083701557 1101 62-63 -3.867869887902419 1101 64-65 -4.7438875860365 1101 66-67 -8.546662030801148 1101 68-69 -4.151670631391092 1101 70-71 -3.64249614780587 1101 72-73 -3.9348251837078347 1101 74-75 -6.009727481851186 1101 76-77 -7.364125408231487 1101 78-79 -10.41058698080705 1101 80-81 -7.062490172732662 1101 82-83 -3.560611709683016 1101 84-85 -2.8529046660984356 1101 86-87 -2.813912135000521 1101 88-89 -3.1126190406896193 1101 90-91 -9.013009069136258 1101 92-93 -15.88130592025615 1101 94-95 -10.428899096907797 1101 96-97 -8.466136045910815 1101 98-99 -8.300832357273936 1101 100-101 -8.373248482388973 1102 1 0.23752332009429367 1102 2 0.2111481603355152 1102 3 0.2725148225220302 1102 4 0.171232423476134 1102 5 0.41745181450307456 1102 6 0.3604205487110832 1102 7 0.1910181133536426 1102 8 0.4994348451642736 1102 9 0.35037288557777657 1102 10-11 0.3139347187397519 1102 12-13 0.3784449815571804 1102 14-15 0.38262236257553184 1102 16-17 0.4797538786707207 1102 18-19 0.34294819357074857 1102 20-21 0.4517924345115105 1102 22-23 0.7064706107879672 1102 24-25 0.53907865434185 1102 26-27 0.3523210522315807 1102 28-29 0.2383438988332287 1102 30-31 0.2555093502021535 1102 32-33 0.3146760652663687 1102 34-35 0.16676259814545347 1102 36-37 0.23289276112438273 1102 38-39 0.02018427301667458 1102 40-41 0.372897015839321 1102 42-43 0.3495087902347862 1102 44-45 0.35139129868855434 1102 46-47 0.22459461515079937 1102 48-49 0.2513159446948805 1102 50-51 0.5763713620727771 1102 52-53 0.5366621762498696 1102 54-55 0.28177566200655946 1102 56-57 -2.085363228728035 1102 58-59 -9.959278082577061 1102 60-61 -10.522408356042863 1102 62-63 -4.575297142743469 1102 64-65 -5.6119599687640545 1102 66-67 -7.209090838919611 1102 68-69 -4.2178790812202305 1102 70-71 -4.491005001487139 1102 72-73 -5.043924800566073 1102 74-75 -5.5359493934179245 1102 76-77 -7.150107079557985 1102 78-79 -9.625702958854063 1102 80-81 -6.224385688938526 1102 82-83 -3.9516415326093934 1102 84-85 -3.3292223631057887 1102 86-87 -3.1853887840633774 1102 88-89 -3.414252570771424 1102 90-91 -8.873493691689845 1102 92-93 -13.417279825195573 1102 94-95 -10.163897025871243 1102 96-97 -8.73255936578241 1102 98-99 -8.867449836822452 1102 100-101 -8.895701107427804 1103 1 0.28482358478586534 1103 2 0.21470903346160597 1103 3 0.13594940334791872 1103 4 0.22782063223824878 1103 5 0.26388322615187576 1103 6 0.22732104604636305 1103 7 0.07509982352231503 1103 8 0.2036331145799295 1103 9 0.38477091997580715 1103 10-11 0.27007663140339844 1103 12-13 0.3678454492185139 1103 14-15 0.5597698658099901 1103 16-17 0.3144314416091518 1103 18-19 0.38577659509479645 1103 20-21 0.3939045070583589 1103 22-23 0.39200693762864347 1103 24-25 0.5031049335855187 1103 26-27 0.4796875491633017 1103 28-29 0.4273075660577703 1103 30-31 0.28539694530713433 1103 32-33 0.021052402077494037 1103 34-35 0.18433847441698248 1103 36-37 0.14248219245294536 1103 38-39 0.454809609815932 1103 40-41 0.5750477832074807 1103 42-43 0.6198888540931122 1103 44-45 0.28135189169262276 1103 46-47 0.06928119896782192 1103 48-49 -0.1507422399720042 1103 50-51 -0.16073530981650208 1103 52-53 -2.9473717830796886E-6 1103 54-55 -0.1624699670216856 1103 56-57 -3.3007930854622423 1103 58-59 -10.671467544766525 1103 60-61 -11.11383970834378 1103 62-63 -5.23795189235474 1103 64-65 -6.623194523476869 1103 66-67 -7.003693742218168 1103 68-69 -4.822488631481955 1103 70-71 -4.600421730469085 1103 72-73 -4.869243201971429 1103 74-75 -5.599656381037956 1103 76-77 -7.3555694589334095 1103 78-79 -9.376382492142291 1103 80-81 -6.0092792781799425 1103 82-83 -3.9283267158163113 1103 84-85 -3.400285521125472 1103 86-87 -3.2235732135521573 1103 88-89 -3.0716906412529745 1103 90-91 -5.825573241595485 1103 92-93 -10.552593003987013 1103 94-95 -8.364448367726933 1103 96-97 -7.760945459385895 1103 98-99 -7.775389396935925 1103 100-101 -8.041901655802267 1104 1 0.21727538781531308 1104 2 0.28192657134678356 1104 3 -0.0212142234709205 1104 4 0.11949569321417641 1104 5 0.20532247947593874 1104 6 0.10659298246437032 1104 7 0.28066921335403094 1104 8 0.26757815017691655 1104 9 0.1362311889005028 1104 10-11 0.28572669182538135 1104 12-13 0.34901322120692413 1104 14-15 0.4676796061062518 1104 16-17 0.37874633814183767 1104 18-19 0.3253060365366096 1104 20-21 0.3722983220290388 1104 22-23 0.3327550452871506 1104 24-25 0.36380797616043736 1104 26-27 0.2555373344905618 1104 28-29 0.43742590451291363 1104 30-31 0.5906685715191955 1104 32-33 0.29935997297744166 1104 34-35 0.10046441121337324 1104 36-37 0.1617064389678191 1104 38-39 0.2197450886835739 1104 40-41 0.13965925026003134 1104 42-43 0.27541367312544196 1104 44-45 0.1207981570346135 1104 46-47 0.29460576472210676 1104 48-49 0.2131063249846079 1104 50-51 -0.03647822749091034 1104 52-53 -0.25028971742906947 1104 54-55 -0.40035957802002287 1104 56-57 -3.5697586849863647 1104 58-59 -10.568523485806942 1104 60-61 -10.574508321467537 1104 62-63 -4.736680388861512 1104 64-65 -6.00409078004105 1104 66-67 -6.869366897643278 1104 68-69 -4.244262191367895 1104 70-71 -3.9863111096651753 1104 72-73 -4.418691222874539 1104 74-75 -4.741180174004462 1104 76-77 -6.506638902454622 1104 78-79 -8.847905756736033 1104 80-81 -5.160871981181611 1104 82-83 -2.9235918140680823 1104 84-85 -2.5617997011165023 1104 86-87 -2.4732711036403927 1104 88-89 -2.4408911109934053 1104 90-91 -4.79551564896407 1104 92-93 -6.549075916241341 1104 94-95 -7.165912301172417 1104 96-97 -7.338101518543192 1104 98-99 -7.611437032297357 1104 100-101 -7.750621681300513 1105 1 0.37597735854833303 1105 2 0.20140205058940452 1105 3 0.2361940862012979 1105 4 0.20128195352565825 1105 5 0.24867524572650268 1105 6 0.16660205489258573 1105 7 0.1955967179322471 1105 8 0.18885803458746153 1105 9 0.36832070352559043 1105 10-11 0.462105616910641 1105 12-13 0.5059595090717082 1105 14-15 0.6575394874926559 1105 16-17 0.5668312157480528 1105 18-19 0.5781067287292814 1105 20-21 0.4385967713158436 1105 22-23 0.37889758321493616 1105 24-25 0.39114370640690765 1105 26-27 0.6150818649923977 1105 28-29 0.5450753055646445 1105 30-31 0.4180244627172627 1105 32-33 0.48630743689773936 1105 34-35 0.6416036729865269 1105 36-37 0.6123767906084154 1105 38-39 0.7499159527483599 1105 40-41 0.784731577673881 1105 42-43 0.8570806478066473 1105 44-45 0.7937648410620994 1105 46-47 0.7571693977255833 1105 48-49 0.6538314472103792 1105 50-51 0.5344463201477367 1105 52-53 0.7611113006989925 1105 54-55 0.7188588490897416 1105 56-57 1.8906712473064395 1105 58-59 -8.330342003640983 1105 60-61 -9.621406055040563 1105 62-63 -3.3047597222060467 1105 64-65 -4.45540796221205 1105 66-67 -6.443426473255247 1105 68-69 -3.8214534347945843 1105 70-71 -4.159625570107709 1105 72-73 -2.6455842522255253 1105 74-75 -4.558596716065246 1105 76-77 -5.716241395692302 1105 78-79 -8.369644302795406 1105 80-81 -4.1787127432655815 1105 82-83 -1.6123470433149016 1105 84-85 -1.0571366410200689 1105 86-87 -0.9150093136839104 1105 88-89 -1.145142551661408 1105 90-91 -4.02440629260245 1105 92-93 -7.2681397760555235 1105 94-95 -8.337486199460416 1105 96-97 -8.275607608830654 1105 98-99 -8.352097471470087 1105 100-101 -8.125969037695736 1106 1 0.37111309902482503 1106 2 0.30825500292218777 1106 3 0.3482978378043562 1106 4 0.21623779045646074 1106 5 0.2107040375188589 1106 6 0.29675396140054744 1106 7 0.2054191625252102 1106 8 0.1673545033481858 1106 9 0.3041876182520369 1106 10-11 0.4145332310211529 1106 12-13 0.4238053628007279 1106 14-15 0.35357384256735 1106 16-17 0.3597705434578984 1106 18-19 0.631032870737485 1106 20-21 0.6072030607149017 1106 22-23 0.4885764300982345 1106 24-25 0.7933952101187671 1106 26-27 0.7907072826901427 1106 28-29 0.6460777801415176 1106 30-31 0.6437320652676988 1106 32-33 0.6506699172546533 1106 34-35 0.520061307146527 1106 36-37 0.6252545248352419 1106 38-39 0.8117731497933178 1106 40-41 0.7699825575702093 1106 42-43 0.5833472879814892 1106 44-45 0.5085843053252361 1106 46-47 0.751673850157708 1106 48-49 0.8173485976538188 1106 50-51 0.977449770105288 1106 52-53 0.7914600896013297 1106 54-55 0.43742540075782443 1106 56-57 1.7877640710334752 1106 58-59 -7.68786236922811 1106 60-61 -9.322773394238222 1106 62-63 -3.52399549338897 1106 64-65 -5.190816989902022 1106 66-67 -6.7121564914984795 1106 68-69 -3.2851114735569347 1106 70-71 -3.2312880522708856 1106 72-73 -3.9336250014623797 1106 74-75 -4.517805032088585 1106 76-77 -6.300923403531472 1106 78-79 -9.006175921385438 1106 80-81 -4.481835767668159 1106 82-83 -1.9898609063642283 1106 84-85 -1.6283782644870044 1106 86-87 -1.4315347909882448 1106 88-89 -1.4922471160816855 1106 90-91 -3.80375531631865 1106 92-93 -5.957594827263009 1106 94-95 -6.877336821717158 1106 96-97 -7.207829489870337 1106 98-99 -6.680357320105456 1106 100-101 -7.440905908891299 1107 1 0.08325608752154423 1107 2 0.06884282547562393 1107 3 0.030892584109036392 1107 4 0.04382067282946167 1107 5 0.07982407122705837 1107 6 0.10991265956391061 1107 7 -0.03125422316772841 1107 8 0.07275013734990665 1107 9 0.15623394561085036 1107 10-11 0.23807130718955705 1107 12-13 0.06313737613404413 1107 14-15 0.22264209989950956 1107 16-17 0.17701243375982756 1107 18-19 0.34801070152157365 1107 20-21 0.1802577419241871 1107 22-23 0.19339752486507678 1107 24-25 0.2597039651468833 1107 26-27 0.34963415480784477 1107 28-29 0.24451181257496302 1107 30-31 0.29910493551788875 1107 32-33 0.12105561554835731 1107 34-35 0.0011647152818454742 1107 36-37 0.31121562827908633 1107 38-39 0.2011788105580692 1107 40-41 0.05248160684882208 1107 42-43 0.2712196538583598 1107 44-45 0.18450971877744848 1107 46-47 0.09518600243024622 1107 48-49 -0.03428351869662549 1107 50-51 -0.16256783386256757 1107 52-53 -0.055883514498646036 1107 54-55 -0.06030358026524141 1107 56-57 1.2818945939213116 1107 58-59 -8.649138996122186 1107 60-61 -10.358561582439993 1107 62-63 -3.7561384461778395 1107 64-65 -5.444362332616997 1107 66-67 -7.75312588808945 1107 68-69 -4.492176806416545 1107 70-71 -4.44471384511896 1107 72-73 -2.481059303490049 1107 74-75 -4.400587780200084 1107 76-77 -6.46326555253674 1107 78-79 -7.446779491098759 1107 80-81 -2.4035231062017424 1107 82-83 -1.035680321076935 1107 84-85 -0.8515058789708227 1107 86-87 -0.720027402142307 1107 88-89 -0.6815236929847828 1107 90-91 -0.3340373547367186 1107 92-93 -3.0697969122441116 1107 94-95 -5.6052912442231 1107 96-97 -6.366009889476837 1107 98-99 -6.247358628797995 1107 100-101 -6.952117279697635 1108 1 -0.06469982141456043 1108 2 -0.06371854310261682 1108 3 -0.034074930496291955 1108 4 0.04520588673531023 1108 5 0.04197301938142317 1108 6 -0.04446009724099298 1108 7 -0.13575764199353557 1108 8 0.02130237596037432 1108 9 -0.2259279050087315 1108 10-11 -0.24719401203184077 1108 12-13 -0.08140134436057878 1108 14-15 -0.07339743272997623 1108 16-17 -0.14467156718329477 1108 18-19 0.04311136694821016 1108 20-21 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2312 76-77 3.8479570109430803 2312 78-79 5.95734467461374 2312 80-81 3.0681168976817084 2312 82-83 1.618821787853932 2312 84-85 1.036866414747692 2312 86-87 1.0948782777162869 2312 88-89 0.9666385244053828 2312 90-91 2.1503617964513566 2312 92-93 4.365189919459045 2312 94-95 10.583473093347614 2312 96-97 12.94112271966438 2312 98-99 12.718299778338931 2312 100-101 11.560722002356648 2313 1 -0.3293256717546953 2313 2 -0.5405892914019361 2313 3 -0.3310137060064946 2313 4 -0.28659683435312644 2313 5 -0.594506572455316 2313 6 -0.2897832264926947 2313 7 -0.42929505695952486 2313 8 -0.42997313424370986 2313 9 -0.38102994582505545 2313 10-11 -0.42534027053524426 2313 12-13 -0.4814863783741785 2313 14-15 -0.436832806879643 2313 16-17 -0.5142293903125505 2313 18-19 -0.5048478167252597 2313 20-21 -0.5163274711083972 2313 22-23 -0.6938296895123344 2313 24-25 -0.5315294537662538 2313 26-27 -0.5591064466959139 2313 28-29 -0.665801317811983 2313 30-31 -0.7217482645554654 2313 32-33 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12.594385212512599 2313 100-101 12.11353312680643 2314 1 -0.31717830095803734 2314 2 -0.5366849808625567 2314 3 -0.3614639429733195 2314 4 -0.3754706777293393 2314 5 -0.2895810477430629 2314 6 -0.48649390128393577 2314 7 -0.4401391102206418 2314 8 -0.5461545831265795 2314 9 -0.4361755111601937 2314 10-11 -0.47736013061197724 2314 12-13 -0.627785182255927 2314 14-15 -0.7628258107162438 2314 16-17 -0.804152658197907 2314 18-19 -0.5892333956013687 2314 20-21 -0.7619971844913849 2314 22-23 -0.631151964393851 2314 24-25 -0.5048509387583593 2314 26-27 -0.9059158576640911 2314 28-29 -0.8015917714341896 2314 30-31 -0.8394446084958886 2314 32-33 -0.9018319119051128 2314 34-35 -0.8658007709108091 2314 36-37 -0.9836917143463424 2314 38-39 -0.9121431966330604 2314 40-41 -0.8400422267207759 2314 42-43 -0.854022940548127 2314 44-45 -0.9838971888511168 2314 46-47 -1.0226745734690965 2314 48-49 -0.8451252832629379 2314 50-51 -0.8223964816002791 2314 52-53 -1.0029728379112157 2314 54-55 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2315 78-79 6.6570454935694805 2315 80-81 3.2804711534634414 2315 82-83 1.9411528863583136 2315 84-85 1.7076538633011218 2315 86-87 1.626419414026408 2315 88-89 1.586533413769068 2315 90-91 2.7942381195437704 2315 92-93 5.03832559972393 2315 94-95 11.407489342446532 2315 96-97 13.32516442298831 2315 98-99 13.235693017222928 2315 100-101 12.376138421631943 2316 1 -0.6493150107738863 2316 2 -0.6981230511745018 2316 3 -0.6080037557577391 2316 4 -0.5153459459380585 2316 5 -0.6047233457317915 2316 6 -0.6502132193853711 2316 7 -0.6228629318706851 2316 8 -0.5736120823602704 2316 9 -0.5167442315393416 2316 10-11 -0.8380979109048212 2316 12-13 -0.6689419576208024 2316 14-15 -3.3413814920253415 2316 16-17 -1.1718697598932195 2316 18-19 -0.9700308302291774 2316 20-21 -0.6355971939228979 2316 22-23 -0.6535311820496474 2316 24-25 -0.6312185084926227 2316 26-27 -1.0978324594890907 2316 28-29 -0.9730596689136135 2316 30-31 -0.846721612103444 2316 32-33 -0.7435239257694448 2316 34-35 -0.6880903891702204 2316 36-37 -0.7385843129646332 2316 38-39 -0.6814483297353249 2316 40-41 -0.7255465770072576 2316 42-43 -0.5215973905728859 2316 44-45 -0.5544567135027378 2316 46-47 -0.8461048156232565 2316 48-49 -0.4249984293806932 2316 50-51 -0.5482812756395603 2316 52-53 -0.47070526581906336 2316 54-55 -0.41269119290806344 2316 56-57 1.478349737970003 2316 58-59 6.0078065393583024 2316 60-61 5.106855235907297 2316 62-63 1.3234579556384922 2316 64-65 0.8731650816595007 2316 66-67 7.165376912413812 2316 68-69 13.811316158795904 2316 70-71 14.23778348849538 2316 72-73 12.86009675830623 2316 74-75 11.355931293238879 2316 76-77 9.431881625565048 2316 78-79 6.971082259199811 2316 80-81 3.017759441266307 2316 82-83 1.532290160277526 2316 84-85 1.1723183567185131 2316 86-87 1.0790883796675175 2316 88-89 1.31449109827075 2316 90-91 2.5239784072747895 2316 92-93 4.48452869974728 2316 94-95 11.013801194737425 2316 96-97 12.858113396532142 2316 98-99 12.519394096663273 2316 100-101 10.981567952677075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 12.0 5 51.0 6 106.0 7 231.0 8 514.0 9 857.0 10 1376.0 11 2193.0 12 3162.0 13 4210.0 14 5277.0 15 7061.0 16 9710.0 17 12688.0 18 13976.0 19 13990.0 20 14550.0 21 14633.0 22 14329.0 23 12279.0 24 12355.0 25 14387.0 26 19831.0 27 29253.0 28 41574.0 29 55496.0 30 66850.0 31 71541.0 32 69647.0 33 62384.0 34 55747.0 35 49273.0 36 37782.0 37 22110.0 38 6575.0 39 250.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04899604745202 18.548293059518752 14.35690342192277 23.045807471106457 2 16.937854954691765 21.698636598760153 35.457276788944846 25.906231657603236 3 21.415774331569434 23.36816913072201 30.11605827972176 25.099998257986794 4 13.930019590869744 16.50282875007035 35.23287942905077 34.33427223000914 5 14.09994431884505 37.125913204484014 33.57104043256878 15.203102044102156 6 32.96331334939552 34.28455805302667 16.095065298432463 16.657063299145346 7 27.67846853534798 32.37116524134826 20.648669509263453 19.3016967140403 8 26.413766949726032 31.392654750211392 19.697930053025054 22.495648247037526 9 24.99685098878952 16.652507282925928 21.10989915124098 37.24074257704358 10-11 26.086810869046694 24.597864568397863 26.46690322217547 22.84842134037997 12-13 27.355594385916014 22.722527892199295 26.634470818716117 23.287406903168577 14-15 24.04351814071035 24.39031253705963 26.064453105370962 25.50171621685906 16-17 22.514894153023185 27.072786379119513 26.712660097069946 23.69965937078736 18-19 22.356334546039275 26.748273066538026 28.03742638338928 22.85796600403342 20-21 23.868097970428774 24.60322458747953 27.436530674049198 24.092146768042493 22-23 23.01762247357835 24.8668364906206 28.140883307895475 23.974657727905576 24-25 24.48081515170194 25.029949106618822 27.44269469599312 23.046541045686123 26-27 23.413331725325452 24.16795642298647 29.342994780674424 23.07571707101365 28-29 22.60975428360468 26.622637426105182 27.901638399644085 22.865969890646053 30-31 23.07942302615091 24.960900278623207 27.601521907235494 24.358154787990387 32-33 23.450711963615216 25.0602348287294 28.011205420196557 23.477847787458828 34-35 24.368057612187112 24.94066850613282 27.519829650604173 23.1714442310759 36-37 23.6650999833674 24.474664799523556 28.695052070758287 23.165183146350753 38-39 23.23130504065237 25.286826727616717 28.28530364701014 23.19656458472077 40-41 23.257249025749722 25.632809932322303 27.94820543433788 23.1617356075901 42-43 22.892010742850395 25.864673354565042 26.74718885576838 24.49612704681618 44-45 23.46780332882827 24.770560360583936 27.162916732342218 24.59871957824557 46-47 24.49382964453387 25.727162977867202 27.01797451374916 22.761032863849763 48-49 23.768931073822216 25.346327735528014 27.144716871417256 23.740024319232518 50-51 22.223392183111248 25.91212237463012 28.34594655499427 23.518538887264356 52-53 23.58339440861138 26.43890114119688 26.175578963461447 23.80212548673029 54-55 22.86279586964203 25.458315929072413 26.77230131150465 24.90658688978091 56-57 22.678107169829627 25.35011052221325 27.671717025406302 24.30006528255083 58-59 22.245169124874412 25.046491302110418 29.014928949310185 23.693410623704985 60-61 23.0116701849205 25.77032246244539 28.231326424353952 22.98668092828016 62-63 22.60312568049716 25.777249200154106 27.692792174073265 23.926832945275464 64-65 22.045308797999393 27.140603240789186 27.06097878380518 23.75310917740624 66-67 21.6862129025648 25.765581776578667 27.649260612683996 24.898944708172536 68-69 21.482183542928567 25.331258419851295 28.794322131697687 24.39223590552246 70-71 21.637573198861762 25.378959389061293 28.703740076760166 24.279727335316775 72-73 21.043327472845068 24.91978241647084 29.637188754743725 24.39970135594037 74-75 21.14972223513869 26.597934337535595 28.087210781270088 24.16513264605563 76-77 20.074060127634095 25.90962570952199 28.355322765808637 25.660991397035282 78-79 21.1660488148068 26.11619222859617 27.9791508821301 24.738608074466935 80-81 23.40516102909531 25.563812999507952 26.31670329110462 24.71432268029212 82-83 22.94317504963046 25.16745045831544 27.194745033771493 24.69462945828261 84-85 22.585810670472288 25.806784453243818 26.733398993655065 24.874005882628826 86-87 22.638153094512738 25.95205855296614 26.425014187329023 24.9847741651921 88-89 22.396854738695545 25.9270326713482 25.33166815295629 26.34444443699997 90-91 21.935368595416595 25.297050685858107 26.48572363219654 26.281857086528753 92-93 21.312123866301246 25.86750406658298 27.502209880081214 25.318162187034567 94-95 19.664816234211553 25.585949385350563 28.716049669718664 26.03318471071922 96-97 19.121980430006957 25.609236036860096 28.714422174289545 26.55436135884341 98-99 19.93920885875915 26.151405557250996 27.242573917582604 26.666811666407252 100-101 20.071944976568503 25.238847040488327 27.381976687554793 27.30723129538838 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 788.0 1 588.0 2 385.5 3 395.0 4 427.5 5 505.0 6 619.5 7 786.5 8 999.5 9 1096.0 10 1089.5 11 1145.5 12 1221.5 13 1249.5 14 1347.5 15 1435.0 16 1444.5 17 1487.0 18 1551.0 19 1658.0 20 1745.0 21 1836.5 22 2026.0 23 2214.0 24 2411.5 25 2637.0 26 2912.5 27 3276.5 28 3627.0 29 3985.5 30 4548.0 31 5284.0 32 6032.0 33 6731.0 34 7487.5 35 8457.0 36 9531.5 37 10679.5 38 12062.0 39 13527.5 40 15002.5 41 16511.0 42 18177.5 43 20304.0 44 23131.5 45 26745.5 46 30894.0 47 35416.0 48 39015.0 49 38990.5 50 36534.5 51 34773.0 52 33702.5 53 32525.0 54 30994.0 55 29220.0 56 27114.0 57 24641.0 58 21945.0 59 18996.0 60 15758.0 61 12511.5 62 9527.0 63 7000.5 64 5105.5 65 3653.0 66 2528.5 67 1752.5 68 1150.5 69 716.0 70 463.0 71 285.5 72 158.5 73 82.0 74 49.0 75 24.5 76 11.0 77 8.0 78 7.0 79 2.0 80 0.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022110078711880213 2 0.005896020989834724 3 4.0200143112509485E-4 4 0.0 5 0.12743445366665507 6 0.0 7 0.0 8 0.002010007155625474 9 0.0 10-11 0.0 12-13 0.0 14-15 3.350011926042457E-4 16-17 0.0 18-19 1.3400047704169828E-4 20-21 0.0 22-23 4.0200143112509485E-4 24-25 0.0 26-27 1.3400047704169828E-4 28-29 0.0026130093023131163 30-31 0.012998046273044734 32-33 0.0026800095408339654 34-35 0.00469001669645944 36-37 0.09942835396494012 38-39 0.09882535181825249 40-41 0.11001439165123428 42-43 0.11256040071502656 44-45 0.10833938568821305 46-47 0.10264436541394088 48-49 0.10257736517542003 50-51 0.10076835873535711 52-53 0.08060128694058151 54-55 0.06961324782316225 56-57 0.5504069594487756 58-59 1.5704855909287039 60-61 0.528899882883583 62-63 0.003819013595688401 64-65 0.11952842552119486 66-67 1.908300793550825 68-69 2.8698212165635306 70-71 2.781782903147135 72-73 2.6329083731538083 74-75 2.019655189972476 76-77 1.4267700793014824 78-79 0.9575674089399757 80-81 0.0545381941559712 82-83 6.700023852084914E-5 84-85 1.3400047704169828E-4 86-87 2.0100071556254743E-4 88-89 6.700023852084914E-5 90-91 0.25547190947999776 92-93 0.40374343732663687 94-95 2.0094041534787865 96-97 2.4336496637928033 98-99 1.4247600721458569 100-101 0.9500633822256407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 746266.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.53457181124447 #Duplication Level Percentage of deduplicated Percentage of total 1 66.3180633741617 26.218562388484568 2 14.966191343450655 11.833639328169504 3 6.561417446436186 7.7820848765905115 4 3.57482361758358 5.653164840875631 5 2.1629295129865547 4.275524607691349 6 1.4447512866200816 3.4270574094161668 7 0.9768593631768158 2.7033801650100253 8 0.7099330758251822 2.245352013391066 9 0.5255199601683986 1.8698585943167898 >10 2.570939175990255 17.083195878031685 >50 0.11144427858489586 3.0201917218934358 >100 0.06523564970502455 4.874698625706713 >500 0.003737459097681905 0.9901185883183993 >1k 0.00747491819536381 6.128365263173291 >5k 6.795380177603463E-4 1.894805698930856 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8604 1.152940104466772 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5502 0.7372706246834239 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3944 0.528497881452458 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3656 0.48990574406444887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3225 0.4321515384594769 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3040 0.4073614502067628 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2569 0.3442472255201228 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2491 0.3337951883108704 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2362 0.3165091267724913 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2293 0.3072630938566141 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2176 0.29158503804273544 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2115 0.2834110089431918 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2064 0.2765769846140652 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2006 0.2688049569456467 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1573 0.2107827503865914 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1536 0.20582473273604857 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1519 0.2035467246263397 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1478 0.19805270506763006 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1432 0.1918886831237119 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1383 0.1853226597486687 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1372 0.18384865450121002 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1211 0.1622745776974966 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1162 0.1557085543224534 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1016 0.13614448467436543 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 886 0.11872442265894467 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 849 0.11376640500840184 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 848 0.11363240453136014 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.3400047704169828E-4 0.0 16-17 0.0 0.0 0.0 2.0100071556254743E-4 0.0 18-19 0.0 0.0 0.0 5.360019081667931E-4 0.0 20-21 0.0 0.0 0.0 8.710031007710388E-4 0.0 22-23 0.0 0.0 0.0 0.001876006678583776 6.700023852084914E-5 24-25 0.0 0.0 0.0 0.003752013357167552 1.3400047704169828E-4 26-27 0.0 0.0 0.0 0.006901024567647461 1.3400047704169828E-4 28-29 0.0 0.0 0.0 0.016616059153170586 1.3400047704169828E-4 30-31 0.0 0.0 0.0 0.03819013595688401 1.3400047704169828E-4 32-33 0.0 0.0 0.0 0.0845543010133116 1.3400047704169828E-4 34-35 0.0 0.0 0.0 0.14760152546143063 1.3400047704169828E-4 36-37 0.0 0.0 0.0 0.23490283625409708 1.3400047704169828E-4 38-39 0.0 0.0 0.0 0.3563072684538757 1.3400047704169828E-4 40-41 0.0 0.0 0.0 0.48387572259757244 1.3400047704169828E-4 42-43 0.0 0.0 0.0 0.6095681700626854 1.3400047704169828E-4 44-45 0.0 0.0 0.0 0.7578396979093245 1.3400047704169828E-4 46-47 0.0 0.0 0.0 0.9260772968351767 1.3400047704169828E-4 48-49 0.0 0.0 0.0 1.0588047693449787 1.3400047704169828E-4 50-51 0.0 0.0 0.0 1.1926042456711148 1.3400047704169828E-4 52-53 0.0 0.0 0.0 1.3270737243824589 1.3400047704169828E-4 54-55 0.0 0.0 0.0 1.4736032460275559 1.3400047704169828E-4 56-57 0.0 0.0 0.0 1.6110207352338173 1.3400047704169828E-4 58-59 0.0 0.0 0.0 1.6888080121565232 1.3400047704169828E-4 60-61 0.0 0.0 0.0 1.751051233742392 1.3400047704169828E-4 62-63 0.0 0.0 0.0 1.8065944314761762 2.6800095408339655E-4 64-65 0.0 1.3400047704169828E-4 0.0 1.8621376292099598 2.6800095408339655E-4 66-67 0.0 1.3400047704169828E-4 0.0 1.9148668169258682 2.6800095408339655E-4 68-69 0.0 2.0100071556254743E-4 0.0 1.9901080847847816 2.6800095408339655E-4 70-71 0.0 2.6800095408339655E-4 0.0 2.097911468564828 2.6800095408339655E-4 72-73 0.0 2.6800095408339655E-4 0.0 2.2394159723208613 2.6800095408339655E-4 74-75 0.0 2.6800095408339655E-4 0.0 2.440081686690805 2.6800095408339655E-4 76-77 0.0 2.6800095408339655E-4 0.0 2.7349497364210618 2.6800095408339655E-4 78-79 0.0 2.6800095408339655E-4 0.0 3.2409355377305142 2.6800095408339655E-4 80-81 0.0 2.6800095408339655E-4 0.0 3.8133855756526494 2.6800095408339655E-4 82-83 0.0 2.6800095408339655E-4 0.0 4.345903471416358 2.6800095408339655E-4 84-85 0.0 2.6800095408339655E-4 0.0 4.766597969088769 2.6800095408339655E-4 86-87 0.0 2.6800095408339655E-4 0.0 5.084715101585761 2.6800095408339655E-4 88-89 0.0 2.6800095408339655E-4 0.0 5.396869212854398 2.6800095408339655E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGCG 185 1.0059011E-8 25.397213 5 AACGCGT 50 0.0014268061 24.36003 62-63 GAACGAC 145 2.559173E-5 22.658022 6 CTTACGC 260 0.0 22.48618 62-63 TAGGACA 230 4.107278E-9 22.47101 4 GCTTACG 300 0.0 22.441038 60-61 TACCGCG 55 0.0024837733 22.14548 62-63 TACATGG 2510 0.0 21.9013 2 GAGTACT 2965 0.0 21.528122 12-13 GTACATG 2590 0.0 21.416277 1 GTACTTT 3050 0.0 20.697334 14-15 TAGATAG 160 5.4230426E-5 20.555868 5 AGTACTT 3070 0.0 20.333176 12-13 ACATGGG 2640 0.0 20.110935 3 GTGCTAT 215 1.1911616E-6 19.67731 1 TTACGCT 200 0.0 19.488024 62-63 CAACGGA 75 6.510901E-4 19.488022 62-63 AGAGTAC 4520 0.0 19.4695 10-11 ATCAACT 950 0.0 19.235174 60-61 CAACTTA 190 7.2759576E-12 19.231604 62-63 >>END_MODULE