FastQCFastQC Report
Fri 27 May 2016
SRR939702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734417
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32470.44211939538436607No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20410.27790751031089966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.21173257154995048No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14970.20383515087477552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.1522295916352699No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10650.14501298308726512No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9290.1264948932282341No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8900.12118455863630608No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8410.11451259979003756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.1109723767287522No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7880.10729599124203279No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7490.10198565665010478No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1653.6567787E-430.7426871
TAAGGCG1701.1641532E-1030.54225
GTACATG19200.028.5330561
GTATTAA2955.1416464E-727.5120981
TACATGG21450.026.213262
ACATGGG22500.023.9399743
CATGGGG14450.022.5591774
GAGTACT26250.022.03041312-13
AGTACTT27900.021.48895812-13
GTGTAGC3804.6574824E-621.3580761
AGAGTAC39100.020.94709410-11
GTTTAAG2450.003664122420.7042581
TTTGCGT2109.045798E-720.2499793
GTACTTT28550.019.59489414-15
GTGCTAG3156.8773504E-419.3239751
GGGTAGG2700.006427364418.7871951
TACTTTT32300.018.56230414-15
GAATCGA1300.00784036918.1544576
ATTAAGG2905.529182E-817.9223963
ACAGCGC1851.5963605E-417.8588398
Produced by FastQC (version 0.11.5)