##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939702_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 734417 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42784412670186 31.0 31.0 34.0 30.0 34.0 2 31.617092196939886 31.0 31.0 34.0 30.0 34.0 3 31.66390075393135 31.0 31.0 34.0 30.0 34.0 4 35.46283923166266 37.0 35.0 37.0 33.0 37.0 5 35.19717953151956 37.0 35.0 37.0 32.0 37.0 6 35.21601760307836 37.0 35.0 37.0 32.0 37.0 7 35.12534976723033 37.0 35.0 37.0 32.0 37.0 8 35.10056275930432 37.0 35.0 37.0 32.0 37.0 9 36.674219142530745 39.0 35.0 39.0 32.0 39.0 10-11 36.54335275463395 38.0 35.0 39.0 32.0 39.0 12-13 36.49745716670502 38.0 35.0 39.0 32.0 39.0 14-15 37.50733098498537 39.0 36.0 41.0 32.0 41.0 16-17 37.458883713203804 39.0 36.0 41.0 32.0 41.0 18-19 37.44151959990033 39.0 36.0 41.0 32.0 41.0 20-21 37.35930540823537 39.0 36.0 41.0 31.5 41.0 22-23 37.21750585838836 39.0 36.0 40.5 31.0 41.0 24-25 37.03957628976453 39.0 36.0 40.0 31.0 41.0 26-27 36.71053502301825 38.5 35.5 40.0 30.0 41.0 28-29 36.520038343339 38.0 35.0 40.0 30.0 41.0 30-31 36.2555115145755 38.0 35.0 40.0 30.0 41.0 32-33 36.03248154658729 38.0 35.0 40.0 30.0 41.0 34-35 35.94611303932235 38.0 35.0 40.0 29.5 41.0 36-37 35.851610869574095 38.0 35.0 40.0 29.0 41.0 38-39 35.862594411621735 38.0 35.0 40.0 29.0 41.0 40-41 35.77585690418387 38.0 35.0 40.0 29.0 41.0 42-43 35.54267194250678 38.0 34.0 40.0 27.5 41.0 44-45 35.28663552178122 38.0 34.0 40.0 27.0 41.0 46-47 34.94681904149822 38.0 33.5 40.0 26.0 41.0 48-49 34.58905294948238 38.0 33.0 40.0 25.0 41.0 50-51 34.2704029182331 37.5 33.0 40.0 24.0 41.0 52-53 34.03641391743383 37.0 33.0 40.0 24.0 41.0 54-55 33.717639297565285 37.0 33.0 40.0 23.0 41.0 56-57 32.901098422285976 36.5 31.5 39.5 19.5 41.0 58-59 29.87129110573421 34.5 25.5 38.5 7.5 40.0 60-61 29.495124023545205 34.0 25.5 38.0 9.0 40.0 62-63 31.128553669100796 34.5 29.5 38.0 12.5 40.0 64-65 31.104631973388415 35.0 30.0 38.0 7.0 40.0 66-67 26.40334714474202 31.0 18.5 36.0 2.0 38.5 68-69 19.131884202026914 19.0 7.0 31.5 2.0 36.0 70-71 17.177944546490618 15.0 7.0 28.5 2.0 34.5 72-73 18.875071655476383 19.5 7.5 30.5 2.0 35.0 74-75 20.28388640241171 22.5 7.5 31.5 2.0 35.0 76-77 22.03889615844949 27.0 11.0 32.5 2.0 35.0 78-79 24.201833563220895 30.5 12.5 34.0 2.0 35.5 80-81 25.70092059415836 31.5 21.0 35.0 2.0 35.5 82-83 26.73061966158191 32.5 25.0 35.0 2.0 35.0 84-85 26.772004869168335 33.0 25.5 35.0 2.0 35.0 86-87 26.561333002912512 33.0 25.0 35.0 2.0 35.0 88-89 26.143598255487007 33.0 24.5 35.0 2.0 35.0 90-91 24.46366097190016 31.5 11.0 34.5 2.0 35.0 92-93 23.000840122164927 31.0 2.0 34.0 2.0 35.0 94-95 19.38950623419665 26.5 2.0 34.0 2.0 35.0 96-97 16.774462600947416 15.5 2.0 32.5 2.0 35.0 98-99 17.03023554737976 19.0 2.0 33.0 2.0 35.0 100-101 16.263345619722855 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23921182415958597 1101 2 0.19988946842794775 1101 3 0.25768555391554315 1101 4 0.18235151200195077 1101 5 0.36332140636393007 1101 6 0.21359262873298235 1101 7 0.33789844087165477 1101 8 0.4485601203098213 1101 9 0.4544680868859956 1101 10-11 0.41545770620003 1101 12-13 0.25229952368387387 1101 14-15 0.4290746345984644 1101 16-17 0.27311208096349304 1101 18-19 0.36361850127483564 1101 20-21 0.40933384544932494 1101 22-23 0.45850141152752144 1101 24-25 0.6234155697443811 1101 26-27 0.6773085399865337 1101 28-29 0.5301532774927225 1101 30-31 0.435044854689032 1101 32-33 0.4298901347651878 1101 34-35 0.3403605070713098 1101 36-37 -0.08298360029876761 1101 38-39 -0.013503318729554792 1101 40-41 0.12364235228616849 1101 42-43 0.3330208255150282 1101 44-45 0.3443333116025613 1101 46-47 0.13936561435485828 1101 48-49 -0.05063216522039937 1101 50-51 0.07504962760656753 1101 52-53 -0.060449517553365695 1101 54-55 0.21004159551773682 1101 56-57 -2.9889291382450445 1101 58-59 -9.676238349111895 1101 60-61 -11.775414856052798 1101 62-63 -3.7535627549461523 1101 64-65 -0.6983911706507513 1101 66-67 -1.5840565128611033 1101 68-69 -3.9789470392287356 1101 70-71 -4.314385591462884 1101 72-73 -3.342955654282031 1101 74-75 -5.60287782637543 1101 76-77 -4.266066647707035 1101 78-79 1.343727538378591 1101 80-81 1.8760568089329084 1101 82-83 0.5046164779620739 1101 84-85 0.15915502436330797 1101 86-87 0.22453133860712882 1101 88-89 0.02046454053610347 1101 90-91 1.2003519113461003 1101 92-93 0.5729861640972871 1101 94-95 -8.382270186677523 1101 96-97 -9.652233615152682 1101 98-99 -9.093867572738155 1101 100-101 -9.388617045909575 1102 1 0.21056924502056518 1102 2 0.287210038307677 1102 3 0.2639962665516471 1102 4 0.17063775518548852 1102 5 0.3010181126195022 1102 6 -0.0733935181338623 1102 7 0.27859206427697103 1102 8 0.29281352272382577 1102 9 0.18205834350374062 1102 10-11 0.28059285391546496 1102 12-13 0.3317706412081449 1102 14-15 0.1045455659343304 1102 16-17 0.12931376596852573 1102 18-19 0.17900554041379024 1102 20-21 0.19835374254442684 1102 22-23 0.18910469775279637 1102 24-25 0.21304290649832325 1102 26-27 0.18782728951658356 1102 28-29 0.13388528077251038 1102 30-31 0.23900458070563957 1102 32-33 -0.022194996300704872 1102 34-35 -0.05455941401345399 1102 36-37 -0.1224349532081419 1102 38-39 0.18491712332467358 1102 40-41 0.225391628120704 1102 42-43 -0.017295336712365383 1102 44-45 0.012481130870909851 1102 46-47 0.14825252996496374 1102 48-49 0.0840044272454179 1102 50-51 0.2188640550593277 1102 52-53 -0.13906219913181417 1102 54-55 -0.08603734836436416 1102 56-57 -3.6005383431131506 1102 58-59 -9.43482986966244 1102 60-61 -7.714511555863389 1102 62-63 -2.290580231592042 1102 64-65 -0.912790695154218 1102 66-67 -1.6589642242834053 1102 68-69 -4.34785518747767 1102 70-71 -3.977349675004188 1102 72-73 -3.2845551558700024 1102 74-75 -3.8455377067135768 1102 76-77 -3.7423876007052073 1102 78-79 1.718657270159877 1102 80-81 2.086331544241034 1102 82-83 0.7787135537300109 1102 84-85 0.33653617708434425 1102 86-87 0.38679363509783826 1102 88-89 0.21698639798023933 1102 90-91 1.3966965317544968 1102 92-93 0.7317699599775089 1102 94-95 -9.171530875502997 1102 96-97 -9.615577773670621 1102 98-99 -8.698845606087364 1102 100-101 -9.261112006290832 1103 1 0.11683911828711047 1103 2 0.037396857556046825 1103 3 0.22816576854129522 1103 4 0.22489714970556918 1103 5 0.17758583396447136 1103 6 0.2178791974842227 1103 7 0.12366619314261129 1103 8 0.13550243789017458 1103 9 0.11021516708733259 1103 10-11 0.19480697323349716 1103 12-13 0.32606400899831556 1103 14-15 0.15678832468532278 1103 16-17 0.32268307430715737 1103 18-19 0.2938913655982063 1103 20-21 0.29838849176805127 1103 22-23 0.12557265846255916 1103 24-25 0.16686101961548871 1103 26-27 0.1976439451905705 1103 28-29 0.16438531535304435 1103 30-31 0.08312647963263231 1103 32-33 0.038283834505996595 1103 34-35 0.13404031035435082 1103 36-37 0.11850874124160526 1103 38-39 0.13090822684868186 1103 40-41 -0.01279068384972959 1103 42-43 0.010191130489268119 1103 44-45 0.03059880253798042 1103 46-47 0.04129745598791601 1103 48-49 -0.1134082737645059 1103 50-51 -0.1792448112219489 1103 52-53 -0.19742908683277705 1103 54-55 -0.2529795347652737 1103 56-57 -0.0735132308950881 1103 58-59 -9.66658526211781 1103 60-61 -8.469151673673377 1103 62-63 -3.0913254843589044 1103 64-65 -1.0727130323262735 1103 66-67 -2.5248027011203504 1103 68-69 -3.9306781064461926 1103 70-71 -4.416045128422516 1103 72-73 -3.536274483354445 1103 74-75 -4.217264152728296 1103 76-77 -0.8569529233416233 1103 78-79 2.6978600285060175 1103 80-81 1.9811468251277802 1103 82-83 0.4996696971734558 1103 84-85 -0.2360374077703895 1103 86-87 -0.007857177376124014 1103 88-89 -0.06286208865733656 1103 90-91 1.3798946073897262 1103 92-93 0.37173342268703635 1103 94-95 -8.349594500319341 1103 96-97 -8.905132426945904 1103 98-99 -8.463761204271632 1103 100-101 -9.520342901386973 1104 1 0.14176071527123213 1104 2 0.07360036416140048 1104 3 0.15102532558456616 1104 4 -0.05075647958222618 1104 5 0.20640077034834547 1104 6 -0.10629493033300008 1104 7 0.06544646970620249 1104 8 0.1343877338717192 1104 9 0.2938017191420599 1104 10-11 0.1733854818700209 1104 12-13 0.08571448591614228 1104 14-15 0.1994623143832399 1104 16-17 0.10043936686008692 1104 18-19 0.218448977596708 1104 20-21 0.20843707726389482 1104 22-23 0.12305319989022223 1104 24-25 0.07909107762559842 1104 26-27 0.04396428447657996 1104 28-29 -0.11006505106933417 1104 30-31 -7.241240299649121E-4 1104 32-33 0.20890748378046453 1104 34-35 -0.061158825181308885 1104 36-37 -0.3274167393323282 1104 38-39 -0.33727802296851195 1104 40-41 -0.3016672051932261 1104 42-43 -0.39736237042882294 1104 44-45 -0.3100367087471767 1104 46-47 -0.233057039389152 1104 48-49 -0.5591566366445306 1104 50-51 -0.345072470830992 1104 52-53 -0.5006350795855852 1104 54-55 -0.46289325082448585 1104 56-57 -0.07687277981369789 1104 58-59 -8.749357722753803 1104 60-61 -7.702069565810326 1104 62-63 -2.663691061778838 1104 64-65 -0.9513142900243956 1104 66-67 -1.9848894725410275 1104 68-69 -3.23147351113575 1104 70-71 -3.8968692944256667 1104 72-73 -2.993457499555028 1104 74-75 -3.9353529065672 1104 76-77 -0.4423626501903932 1104 78-79 2.9220447183875606 1104 80-81 2.5331861160070304 1104 82-83 1.0054097728953337 1104 84-85 0.7328435008921268 1104 86-87 0.7453691270388205 1104 88-89 0.49999925581220594 1104 90-91 1.4696169063448927 1104 92-93 0.7902730954255368 1104 94-95 -6.624142362720018 1104 96-97 -7.761065057224904 1104 98-99 -6.001567707253384 1104 100-101 -5.673016728591602 1105 1 0.4068397676280284 1105 2 0.41402624539405863 1105 3 0.42561036227865046 1105 4 0.33728141301164527 1105 5 0.5019449428023322 1105 6 0.4211312300045478 1105 7 0.3314186577243916 1105 8 0.4598798614719044 1105 9 0.4130960762978191 1105 10-11 0.3356964703938843 1105 12-13 0.3991106999403371 1105 14-15 0.4293741500725474 1105 16-17 0.5056898224577822 1105 18-19 0.6353364654884857 1105 20-21 0.8288675555183431 1105 22-23 0.7685891633767383 1105 24-25 0.8323139182566948 1105 26-27 0.8144645389545744 1105 28-29 0.8526916843884749 1105 30-31 0.5406121316957879 1105 32-33 0.543313729162918 1105 34-35 0.875127273753165 1105 36-37 0.7298457341937521 1105 38-39 0.7220457222908081 1105 40-41 0.6478271224102556 1105 42-43 0.759482266652725 1105 44-45 0.8548313731454797 1105 46-47 1.131018056245324 1105 48-49 1.3737547557605296 1105 50-51 1.2573229058785245 1105 52-53 1.246535289325415 1105 54-55 1.157190809002472 1105 56-57 1.8373147786752 1105 58-59 -7.314018117769322 1105 60-61 -6.710450921603396 1105 62-63 -0.9182503223150462 1105 64-65 1.003782657881498 1105 66-67 -0.3878791784958153 1105 68-69 -2.4015510037887218 1105 70-71 -2.843139960501288 1105 72-73 -2.1918107806793294 1105 74-75 -2.7303733468962186 1105 76-77 0.6151953461648247 1105 78-79 4.584919662977029 1105 80-81 4.064758625816744 1105 82-83 2.81504792160899 1105 84-85 2.550446810747811 1105 86-87 2.336690606623119 1105 88-89 2.266487787884639 1105 90-91 3.648253889385167 1105 92-93 3.3904610807807387 1105 94-95 -5.124486318457265 1105 96-97 -6.723491792190181 1105 98-99 -4.4024550966375475 1105 100-101 -4.203352147002674 1106 1 0.13920488200508885 1106 2 0.23735106963588137 1106 3 -0.004219373278612437 1106 4 0.12395931498360824 1106 5 0.11476971334600705 1106 6 0.16083399008947907 1106 7 0.2273185974172378 1106 8 0.17936072427726657 1106 9 0.14092741453833213 1106 10-11 0.0106103468220482 1106 12-13 0.06604585735853163 1106 14-15 0.12462558133479007 1106 16-17 0.21637905887008202 1106 18-19 0.0821835579401764 1106 20-21 0.0975929266551745 1106 22-23 0.09187929035221032 1106 24-25 0.05979332708137264 1106 26-27 0.05792864793703245 1106 28-29 0.16468702504128174 1106 30-31 0.34167287669566093 1106 32-33 0.2616487560532974 1106 34-35 0.07523962680610907 1106 36-37 0.032036618726209554 1106 38-39 0.1185512118940224 1106 40-41 0.18752492574284219 1106 42-43 0.25346807381853864 1106 44-45 0.06978672106819062 1106 46-47 0.006098810002121979 1106 48-49 0.11515602964588112 1106 50-51 0.05346820481199899 1106 52-53 0.25292454308635826 1106 54-55 0.01621664194339445 1106 56-57 0.8471093212908727 1106 58-59 -7.969925327365594 1106 60-61 -7.2686341066472195 1106 62-63 -1.6485939078644627 1106 64-65 -0.22269218246976763 1106 66-67 0.37353993969794885 1106 68-69 -1.9444905231489713 1106 70-71 -1.829143744155573 1106 72-73 -0.9447918541466578 1106 74-75 -1.8297413609733937 1106 76-77 1.7234359469232103 1106 78-79 3.9794622786552374 1106 80-81 2.8705117630279133 1106 82-83 1.3243608331227001 1106 84-85 1.0870078159771879 1106 86-87 0.884359693837176 1106 88-89 0.870486234768908 1106 90-91 2.219475811448497 1106 92-93 1.7184477305944483 1106 94-95 -6.000128011353413 1106 96-97 -7.840525671592397 1106 98-99 -5.767201021774065 1106 100-101 -5.019610294664325 1107 1 5.265380560430799E-4 1107 2 0.09723960466773107 1107 3 -5.569528964457504E-4 1107 4 -0.1011362264176654 1107 5 0.056226719715432694 1107 6 -0.09178543666211425 1107 7 0.1277724190732883 1107 8 -0.03512175979916776 1107 9 -0.05368245807313343 1107 10-11 -0.12966831559833736 1107 12-13 0.018064169460444646 1107 14-15 0.11445440299084453 1107 16-17 -0.18896727870953356 1107 18-19 -0.10350513632734248 1107 20-21 0.110968722833519 1107 22-23 -0.09951800264141752 1107 24-25 0.05383791306862662 1107 26-27 -0.042815778814564 1107 28-29 -0.1455160004364302 1107 30-31 -0.11142823795402279 1107 32-33 -0.07459726305497583 1107 34-35 -0.11509078720662558 1107 36-37 -0.02596895452219883 1107 38-39 -0.010592896386235395 1107 40-41 -0.4267067621552485 1107 42-43 -0.543992117264267 1107 44-45 -0.3166901897598322 1107 46-47 -0.39490039381953324 1107 48-49 -0.18499524423947378 1107 50-51 0.05591645321964478 1107 52-53 -0.07887083907925074 1107 54-55 -0.3662714153814477 1107 56-57 -0.05706265323141935 1107 58-59 -8.608368798703172 1107 60-61 -7.905811654417921 1107 62-63 -2.6883192263358 1107 64-65 -0.9399851761003397 1107 66-67 0.00856780593998252 1107 68-69 -2.6621776250597993 1107 70-71 -2.2729056868307307 1107 72-73 -0.9514638286689525 1107 74-75 -0.9151155647950482 1107 76-77 1.8478826029741668 1107 78-79 3.8362377563622445 1107 80-81 2.8386845337285465 1107 82-83 1.4113433171991296 1107 84-85 1.170921032537695 1107 86-87 0.9432251396970486 1107 88-89 0.8141685596096728 1107 90-91 1.9755188050790693 1107 92-93 1.6568553739065486 1107 94-95 -6.627520527276978 1107 96-97 -7.673248601528702 1107 98-99 -6.015262327506548 1107 100-101 -5.334701855173881 1108 1 -0.1707234619439575 1108 2 -0.3265103953322708 1108 3 -0.2993069528964476 1108 4 -0.2773862264176685 1108 5 -0.280023280284567 1108 6 -0.21428543666211652 1108 7 -0.10847758092671 1108 8 -0.3463717597991689 1108 9 -0.08743245807313116 1108 10-11 -0.31904331559833565 1108 12-13 -0.2694358305395568 1108 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-1.4777938339543795 2312 100-101 -0.9360214624423442 2313 1 -0.3082615181500472 2313 2 -0.34486226886857096 2313 3 -0.2786014493835651 2313 4 -0.2083025027642691 2313 5 -0.20518135990986508 2313 6 -0.17410686523354713 2313 7 -0.2035127097323297 2313 8 -0.1170040783003401 2313 9 -0.3536444018670437 2313 10-11 -0.2758363483852193 2313 12-13 -0.26811410922807966 2313 14-15 -0.5008193089529556 2313 16-17 -0.22808759486878216 2313 18-19 -0.2185592932360052 2313 20-21 -0.21924205000020436 2313 22-23 -0.37046501669294685 2313 24-25 -0.3593119698353533 2313 26-27 -0.4287686476670203 2313 28-29 -0.28735879903127426 2313 30-31 -0.4549455096167847 2313 32-33 -0.30449334033834674 2313 34-35 -0.5705635623822687 2313 36-37 -0.7292754533512422 2313 38-39 -0.6606646176977122 2313 40-41 -0.5870331673074674 2313 42-43 -0.24870230461789333 2313 44-45 -0.4576547682141694 2313 46-47 -0.7281834734448296 2313 48-49 -0.5208588273776442 2313 50-51 -0.6842255257030772 2313 52-53 -0.5626574315851016 2313 54-55 -0.6379473521495953 2313 56-57 0.5071176746374348 2313 58-59 3.5880700186270396 2313 60-61 3.566947420523533 2313 62-63 0.9511845792848099 2313 64-65 0.03627946090199785 2313 66-67 -2.0576046179476037 2313 68-69 -4.7776152714298235 2313 70-71 -3.612216284020427 2313 72-73 -2.7948537584113406 2313 74-75 -3.498215681891068 2313 76-77 0.42151989805613255 2313 78-79 2.6776282715847266 2313 80-81 2.135003327639552 2313 82-83 0.6832549097049885 2313 84-85 0.4019968522098267 2313 86-87 0.3825152450834679 2313 88-89 0.6698433254176344 2313 90-91 2.193291053322632 2313 92-93 3.52235888678711 2313 94-95 5.805721568741756 2313 96-97 1.2768977684947167 2313 98-99 2.6070781291679257 2313 100-101 3.7960373134443888 2314 1 -0.43313555967935713 2314 2 -0.4183354847244054 2314 3 -0.4371779302504386 2314 4 -0.18316244811015991 2314 5 -0.3088343649091243 2314 6 -0.3599573675322034 2314 7 -0.568024660783685 2314 8 -0.5423431543164554 2314 9 -0.5523698835796864 2314 10-11 -0.37783130725506453 2314 12-13 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-0.04282582968376403 2314 78-79 2.9524222021310145 2314 80-81 2.22073533825775 2314 82-83 0.5900456413945996 2314 84-85 0.31927249260921187 2314 86-87 0.49977385840980304 2314 88-89 0.6544017240911977 2314 90-91 2.126274168607079 2314 92-93 3.7200138959566083 2314 94-95 6.148393507287981 2314 96-97 3.6218795271959685 2314 98-99 6.066629061051261 2314 100-101 6.314282799176537 2315 1 -0.4816503127077638 2315 2 -0.4307950604321533 2315 3 -0.6191527225791731 2315 4 -0.37655338270438676 2315 5 -0.4554668760542526 2315 6 -0.632139431961761 2315 7 -0.5092296373309395 2315 8 -0.7914716422903538 2315 9 -0.8153907424444569 2315 10-11 -0.7142790676547435 2315 12-13 -0.6814224639120567 2315 14-15 -0.7233210082900072 2315 16-17 -0.6701298815297463 2315 18-19 -0.7957532268091256 2315 20-21 -0.9330970821018525 2315 22-23 -0.7726547535227439 2315 24-25 -0.6992701950394817 2315 26-27 -0.7167133992610957 2315 28-29 -0.7864465233506479 2315 30-31 -0.9480050593406233 2315 32-33 -1.0197683852641504 2315 34-35 -0.6643174323300087 2315 36-37 -0.5196433082237277 2315 38-39 -0.7097534428022172 2315 40-41 -0.7496026199695791 2315 42-43 -0.7352039268999846 2315 44-45 -0.8340895728385718 2315 46-47 -0.8265888485786377 2315 48-49 -0.5398762665661465 2315 50-51 -0.4270043752174857 2315 52-53 -0.49198702591827015 2315 54-55 -0.5346152755459528 2315 56-57 -0.1677970833136797 2315 58-59 3.0267597265612203 2315 60-61 3.376177916674912 2315 62-63 0.6507796279532734 2315 64-65 -0.33276425953159716 2315 66-67 -1.9702930930024323 2315 68-69 -4.689330239630891 2315 70-71 -3.4165021909435396 2315 72-73 -3.357935626553793 2315 74-75 -0.7695970571569752 2315 76-77 1.611628930823752 2315 78-79 3.283868484916887 2315 80-81 2.289980802823731 2315 82-83 0.6114094159065324 2315 84-85 0.2999038468737716 2315 86-87 0.3989382713069176 2315 88-89 0.607691620714256 2315 90-91 1.7904174537277164 2315 92-93 3.0639176535775263 2315 94-95 5.533454061442175 2315 96-97 3.302383008342037 2315 98-99 5.3072104985804085 2315 100-101 5.68341873824563 2316 1 -0.577763241270322 2316 2 -0.44129988429858713 2316 3 -0.7138844790288523 2316 4 -0.4579074227010622 2316 5 -0.5048519678571566 2316 6 -0.8518072136656016 2316 7 -0.6176994160022033 2316 8 -0.4794495762916213 2316 9 -0.7246769412322465 2316 10-11 -0.6661564108364288 2316 12-13 -0.46185090022596853 2316 14-15 -0.777651433246092 2316 16-17 -0.7285731149464638 2316 18-19 -0.7120380921926142 2316 20-21 -0.5702159461560257 2316 22-23 -0.9742806333034366 2316 24-25 -0.9256568604505375 2316 26-27 -0.7937369460619479 2316 28-29 -0.839404647358613 2316 30-31 -0.6943354679191813 2316 32-33 -0.6579995568993482 2316 34-35 -0.9280967395875734 2316 36-37 -0.656599761723129 2316 38-39 -1.0731996037033085 2316 40-41 -1.1608131791122744 2316 42-43 -1.1271033251620537 2316 44-45 -1.1768099632790054 2316 46-47 -1.4114197027626219 2316 48-49 -1.3718898435426041 2316 50-51 -1.4100352018326205 2316 52-53 -1.2331892188701872 2316 54-55 -0.7831464153814451 2316 56-57 0.10079448962572002 2316 58-59 3.081382217556989 2316 60-61 2.975741045930512 2316 62-63 0.42447621501147026 2316 64-65 -0.08145439793541698 2316 66-67 -2.076425660958968 2316 68-69 -3.4332149363257685 2316 70-71 -3.386458967899257 2316 72-73 -1.0487141190289968 2316 74-75 4.959244191302513 2316 76-77 5.128651331197162 2316 78-79 4.039078929416831 2316 80-81 2.3048829948203036 2316 82-83 0.7690015634244496 2316 84-85 0.6115082857316558 2316 86-87 0.6349999654810219 2316 88-89 0.7043914109453304 2316 90-91 1.9669720571115938 2316 92-93 3.755008684011081 2316 94-95 5.987253717786903 2316 96-97 3.4145998305270453 2316 98-99 5.596939327545714 2316 100-101 5.900709730191975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 10.0 5 36.0 6 118.0 7 284.0 8 565.0 9 903.0 10 1488.0 11 2202.0 12 3023.0 13 4135.0 14 5118.0 15 6658.0 16 8570.0 17 11093.0 18 11893.0 19 11578.0 20 12251.0 21 12413.0 22 12230.0 23 12619.0 24 13201.0 25 14034.0 26 17717.0 27 24356.0 28 33373.0 29 43949.0 30 52151.0 31 57229.0 32 59484.0 33 59729.0 34 61763.0 35 65065.0 36 61476.0 37 40898.0 38 12312.0 39 486.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.850734234590064 18.70711517036803 14.439810741534714 23.00233985350719 2 16.905773842514733 21.719011505741978 35.526310413996995 25.848904237746297 3 21.328131914516995 23.43879141902058 30.12656331910432 25.1065133473581 4 14.037392925272698 16.514868256045272 35.17579249935663 34.271946319325394 5 14.148107981438136 37.05724149770157 33.52689045175741 15.26776006910289 6 32.897250472143206 34.50219698073438 16.04292928949085 16.557623257631562 7 27.695845820562432 32.26436751872574 20.719427791023357 19.320358869688473 8 26.20336947537979 31.407633537894686 19.887611534046734 22.50138545267879 9 24.931476259400316 16.69936834250841 21.113345687804067 37.25580971028721 10-11 26.05216110193528 24.737172478305922 26.425586553688163 22.78507986607064 12-13 27.384033866318454 22.60575395177399 26.739100538250067 23.271111643657484 14-15 23.90761620311146 24.437902574091332 26.143002350852278 25.51147887194493 16-17 22.516378648900183 27.0665895986814 26.732719104214027 23.684312648204394 18-19 22.400505438364295 26.732610578258527 28.01799262539283 22.848891357984346 20-21 23.811676472630673 24.68597540634272 27.410721701703526 24.09162641932308 22-23 22.968558734343024 24.981788275598195 28.117881258195276 23.931771731863506 24-25 24.387047404299878 25.12736301540066 27.523006359470408 22.962583220829053 26-27 23.37127501901169 24.273487864175163 29.287059863170285 23.068177253642858 28-29 22.61973735361539 26.718608623662952 27.9277433094207 22.733910713300958 30-31 23.038622950998256 25.06556533248364 27.62356648174928 24.27224523476883 32-33 23.452127579900843 25.132526937725615 27.979422069530855 23.435923412842687 34-35 24.389948325515213 25.043130152980986 27.43887909259877 23.128042428905033 36-37 23.65220887091554 24.504549929987974 28.725917396029587 23.117323803066903 38-39 23.285414396938855 25.369850656168513 28.24532751192863 23.099407434963997 40-41 23.279399399563093 25.647554711848887 27.92317787295976 23.149868015628257 42-43 22.905867706592183 25.974606501624148 26.680072311437975 24.439453480345694 44-45 23.42869252826568 24.828961283921544 27.18132284477589 24.561023343036883 46-47 24.45036805664863 25.777320787449753 27.070408081914838 22.701903073986777 48-49 23.747201430251398 25.44153697518726 27.09826301309383 23.712998581467506 50-51 22.269420534922286 25.941714209409717 28.330319757024448 23.45854549864355 52-53 23.61197114641173 26.504687107236354 26.100385363936283 23.782956382415634 54-55 22.811821409207447 25.56930106340371 26.807009800315505 24.811867727073338 56-57 23.553998280426704 25.325349535292812 26.762596691933403 24.35805549234708 58-59 22.998141058739392 25.1204609022345 28.268798380643425 23.61259965838269 60-61 22.487415901054277 25.82985369106242 28.756032442654224 22.92669796522908 62-63 22.628022481166408 25.751760809104123 27.7049397971793 23.91527691255017 64-65 22.506348763267724 27.10169595381232 26.631376847609257 23.7605784353107 66-67 22.34683671692318 25.745845629004915 27.097491855085572 24.809825798986335 68-69 21.1885874298289 25.246814714821713 29.28126523090311 24.28333262444628 70-71 22.124160336876155 25.334104876407864 28.44811779510651 24.09361699160947 72-73 21.95121105779163 24.75808654394097 28.999319217613266 24.291383180654133 74-75 21.53494331857046 26.684063959879563 27.673351107629202 24.107641613920777 76-77 20.984662450441057 25.842467726667856 27.67734899098679 25.4955208319043 78-79 22.307823463474456 25.9055635176655 27.286496953893792 24.500116064966253 80-81 23.508963349059464 25.449223126469008 26.28586139892403 24.755952125547502 82-83 22.980368074769615 25.19886549312651 27.145582340254027 24.67518409184985 84-85 22.571482647952298 25.836693177779686 26.739323728331215 24.8525004459368 86-87 22.687621313119415 25.95745028502413 26.40447487546151 24.950453526394945 88-89 22.445626939463548 25.98516918862172 25.419686635794108 26.149517236120623 90-91 22.31394796793508 25.161127258940986 26.378146122869992 26.146778650253943 92-93 22.615430727939074 25.72405618605158 26.515143258082535 25.14536982792681 94-95 21.092295288284877 25.321284547067318 27.86702253064378 25.719397634004025 96-97 19.974191386652212 25.427853225972825 28.453650070371395 26.144305317003568 98-99 21.320516958193977 25.85537860863118 26.643999553235854 26.180104879938987 100-101 20.17565902948626 25.0495911172158 27.754112964353965 27.020636888943972 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1059.0 1 763.5 2 449.0 3 469.0 4 479.0 5 484.0 6 594.0 7 855.0 8 1114.5 9 1230.5 10 1234.5 11 1233.5 12 1272.0 13 1267.0 14 1290.0 15 1311.0 16 1326.0 17 1389.5 18 1454.5 19 1561.0 20 1628.0 21 1767.5 22 1989.0 23 2123.0 24 2268.0 25 2477.5 26 2783.0 27 3177.0 28 3487.5 29 3869.0 30 4456.0 31 5206.0 32 5964.5 33 6515.0 34 7052.0 35 7770.5 36 8725.5 37 9895.5 38 11655.0 39 13203.5 40 14285.5 41 15642.0 42 17197.0 43 19068.5 44 21502.0 45 24641.0 46 28912.0 47 34684.0 48 39365.0 49 39071.5 50 35806.0 51 33730.0 52 32640.5 53 31562.0 54 30487.0 55 29100.5 56 27233.5 57 24972.0 58 22650.5 59 19915.5 60 16641.0 61 13404.5 62 10327.0 63 7642.5 64 5488.0 65 3895.5 66 2764.0 67 1973.5 68 1320.5 69 839.5 70 570.0 71 359.0 72 192.5 73 95.0 74 53.5 75 32.5 76 14.0 77 5.5 78 3.5 79 3.0 80 2.0 81 1.0 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024917723854431473 2 0.011982293438196557 3 2.7232485086810357E-4 4 0.0 5 0.05977530476554873 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.404060635851294E-4 16-17 6.808121271702589E-5 18-19 6.808121271702588E-4 20-21 0.0 22-23 0.0 24-25 6.808121271702589E-5 26-27 6.808121271702589E-5 28-29 0.0010212181907553884 30-31 0.004765684890191812 32-33 0.005174172166493967 34-35 0.001633949105208621 36-37 0.08367181042922481 38-39 0.07808915098642868 40-41 0.08414837891824399 42-43 0.08802900804311448 44-45 0.09054801291364444 46-47 0.08585040923616964 48-49 0.07625095824306899 50-51 0.0702598115239707 52-53 0.05929873627652955 54-55 0.04888231073082458 56-57 0.06998748667310262 58-59 0.7132868656362802 60-61 0.34598872302792555 62-63 0.0033359794231342683 64-65 0.0029955733595491392 66-67 1.0952224689787955 68-69 2.3597629139848344 70-71 2.3473721332703352 72-73 1.795710066624275 74-75 1.2799267990800867 76-77 0.747123228356642 78-79 0.5749458413952836 80-81 0.03928285973772394 82-83 1.3616242543405179E-4 84-85 0.0010892994034724143 86-87 7.488933398872848E-4 88-89 0.0 90-91 0.33557229748222056 92-93 0.05814135566034011 94-95 0.9511626228695687 96-97 1.6581179357231655 98-99 0.6434355413886117 100-101 0.5712694559085643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 734417.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.02451030691022 #Duplication Level Percentage of deduplicated Percentage of total 1 66.57239580460441 26.645275420370957 2 14.944570413346673 11.962982250826789 3 6.561383959548234 7.878485397496012 4 3.5708373812287557 5.716840702771625 5 2.118603532118023 4.239803445375711 6 1.392889129568866 3.3449823193687367 7 0.9383341323128643 2.628945490505733 8 0.6765085581219856 2.1661539005813113 9 0.5468062314886594 1.9697086483290525 >10 2.49167976212985 16.741599079328942 >50 0.10812646636633466 2.9839414224715295 >100 0.06698398229509836 5.0349744831539995 >500 0.002720161717566518 0.7736797561079287 >1k 0.007480444723307923 6.038240474714634 >5k 6.800404293916295E-4 1.8743872085970983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8571 1.1670481483952577 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5202 0.7083169371079373 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3926 0.5345736822540872 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3595 0.48950391943541616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3029 0.4124359866397428 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2936 0.39977288107437603 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2511 0.341903850264904 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2417 0.32910458227410316 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2272 0.30936103058616565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2157 0.2937023516612497 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2153 0.29315770195951346 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2021 0.27518426180221867 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1923 0.26184034410968154 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1919 0.26129569440794537 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1622 0.22085545405403198 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1551 0.2111879218482143 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1442 0.19634621747590267 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1440 0.19607389262503455 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1415 0.19266983198918325 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1362 0.1854532234411785 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1298 0.1767388282133992 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1189 0.16189712384108756 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1136 0.15468051529308283 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1055 0.14365135883292462 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 957 0.13030744114038753 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 795 0.10824912822007117 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 789 0.10743215366746685 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3616242543405176E-4 0.0 12-13 0.0 0.0 0.0 2.723248508681035E-4 0.0 14-15 0.0 0.0 0.0 2.723248508681035E-4 0.0 16-17 0.0 0.0 0.0 3.404060635851294E-4 0.0 18-19 0.0 0.0 0.0 4.0848727630215533E-4 0.0 20-21 0.0 0.0 0.0 6.12730914453233E-4 0.0 22-23 0.0 0.0 0.0 0.0013616242543405177 0.0 24-25 0.0 0.0 0.0 0.0027913297213980615 0.0 26-27 0.0 0.0 0.0 0.005038009741059915 0.0 28-29 0.0 0.0 0.0 0.015045948010462721 0.0 30-31 0.0 0.0 0.0 0.03519798697470239 0.0 32-33 0.0 0.0 0.0 0.08142513040956295 0.0 34-35 0.0 0.0 0.0 0.1435832776202076 0.0 36-37 0.0 0.0 0.0 0.2280039813893197 0.0 38-39 0.0 0.0 0.0 0.3400656575215443 0.0 40-41 0.0 0.0 0.0 0.4691476368330254 0.0 42-43 0.0 0.0 0.0 0.5970041543155999 0.0 44-45 0.0 0.0 0.0 0.7512761823323806 0.0 46-47 0.0 0.0 0.0 0.9278788481203458 0.0 48-49 0.0 0.0 0.0 1.080993495520937 0.0 50-51 0.0 0.0 0.0 1.2418694011712692 0.0 52-53 0.0 0.0 0.0 1.3906949321706876 0.0 54-55 0.0 0.0 0.0 1.5587193651563078 0.0 56-57 0.0 0.0 0.0 1.743764101321184 0.0 58-59 0.0 0.0 0.0 1.8652890660210752 0.0 60-61 0.0 0.0 0.0 1.9602623577613263 0.0 62-63 0.0 0.0 6.808121271702588E-5 2.046657416699232 0.0 64-65 0.0 0.0 1.3616242543405176E-4 2.1476218551585817 0.0 66-67 0.0 0.0 1.3616242543405176E-4 2.2462715323855518 0.0 68-69 0.0 0.0 1.3616242543405176E-4 2.3560184472853978 0.0 70-71 0.0 0.0 1.3616242543405176E-4 2.5102223940894612 0.0 72-73 0.0 0.0 1.3616242543405176E-4 2.7178020116636734 0.0 74-75 0.0 0.0 1.3616242543405176E-4 3.013751043344585 0.0 76-77 0.0 0.0 1.3616242543405176E-4 3.3643692888372683 0.0 78-79 0.0 0.0 1.3616242543405176E-4 3.861430222884274 0.0 80-81 0.0 0.0 1.3616242543405176E-4 4.410164797383503 0.0 82-83 0.0 0.0 1.3616242543405176E-4 4.921182380037499 0.0 84-85 0.0 0.0 1.3616242543405176E-4 5.38631322532022 0.0 86-87 0.0 0.0 1.3616242543405176E-4 5.817335383031711 0.0 88-89 0.0 0.0 1.3616242543405176E-4 6.243932261916595 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATA 35 0.007592404 27.705833 70-71 CTTACGC 160 0.0 24.240059 62-63 GCTTACG 180 0.0 23.916122 60-61 TACATGG 2270 0.0 23.272964 2 GTACATG 2325 0.0 23.129753 1 ACGCATA 365 0.0 22.625711 64-65 TTACGCG 55 0.002556 22.036419 62-63 CGCATAG 335 0.0 21.751673 66-67 ACATGGG 2325 0.0 21.708027 3 TAAGGTA 135 3.9773923E-4 20.964138 4 AGAGTAC 3725 0.0 20.000576 10-11 GAGTACT 2720 0.0 19.329369 12-13 CATGGGG 1595 0.0 19.222603 4 AACGCAT 420 0.0 19.084503 64-65 AGTACTT 2880 0.0 18.91042 12-13 GGTTGCG 90 1.0384417E-4 18.89173 66-67 TTTGCGT 200 1.3633962E-5 18.867725 3 TATACAG 275 3.0693627E-8 18.867723 5 CTACGTT 65 0.00664218 18.68544 68-69 TAAGGCG 205 1.6866026E-5 18.407536 5 >>END_MODULE