##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939701_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012718 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.163927174198545 31.0 30.0 33.0 26.0 34.0 2 30.25244836173545 31.0 30.0 34.0 26.0 34.0 3 30.264155470723342 31.0 30.0 34.0 26.0 34.0 4 33.86643665857623 35.0 35.0 37.0 28.0 37.0 5 33.90238348681469 35.0 35.0 37.0 28.0 37.0 6 34.047458423766535 36.0 35.0 37.0 29.0 37.0 7 33.85740551663938 35.0 35.0 37.0 28.0 37.0 8 33.87258150837647 35.0 35.0 37.0 28.0 37.0 9 35.09299726083668 37.0 35.0 39.0 28.0 39.0 10-11 35.00727152079848 37.0 34.0 39.0 27.5 39.0 12-13 34.882373474155685 37.0 34.0 39.0 27.0 39.0 14-15 35.46196966974024 38.0 34.0 40.0 27.0 41.0 16-17 35.235374013298866 38.0 33.5 40.0 27.0 41.0 18-19 35.299990718047866 38.0 34.0 40.0 27.0 41.0 20-21 35.43643097091194 38.0 34.0 40.0 27.0 41.0 22-23 35.267524128138334 38.0 34.0 40.0 26.5 41.0 24-25 35.11428502307651 38.0 34.0 40.0 25.5 41.0 26-27 35.13775355034669 38.0 34.0 40.0 25.5 41.0 28-29 35.00338198787817 38.0 34.0 40.0 25.0 41.0 30-31 34.70860249348782 38.0 33.0 40.0 24.5 41.0 32-33 34.37936227064198 38.0 33.0 40.0 24.0 41.0 34-35 34.09106384995626 38.0 33.0 40.0 23.5 41.0 36-37 33.720466605708594 37.5 33.0 40.0 21.0 41.0 38-39 33.50251353288872 37.0 32.5 40.0 20.0 41.0 40-41 33.2161075442522 37.0 31.5 40.0 18.5 41.0 42-43 32.832210941249194 37.0 31.0 40.0 15.5 41.0 44-45 32.47261231655801 37.0 31.0 40.0 14.0 41.0 46-47 31.8922681338734 36.5 30.0 39.5 9.0 41.0 48-49 31.42745956919893 36.0 30.0 39.0 7.5 40.5 50-51 30.77427625459407 35.5 28.5 38.5 6.0 40.0 52-53 30.82605917935694 35.5 29.0 39.0 2.0 40.0 54-55 30.856623956520966 36.0 29.0 39.0 2.0 40.0 56-57 30.70537750884254 35.5 28.5 39.0 2.0 40.0 58-59 30.314912443543022 35.0 28.0 39.0 2.0 40.0 60-61 29.85186893093635 35.0 28.0 38.5 2.0 40.0 62-63 29.452083403277122 34.5 27.0 38.0 2.0 40.0 64-65 28.837002995898168 34.0 26.0 38.0 2.0 40.0 66-67 28.266059258352275 34.0 26.0 37.0 2.0 39.0 68-69 27.733939754206006 33.0 24.5 36.5 2.0 39.0 70-71 27.153915502637457 33.0 23.5 36.0 2.0 39.0 72-73 26.393105978169636 32.0 21.5 35.5 2.0 38.0 74-75 25.943992799575007 32.0 20.0 35.0 2.0 37.5 76-77 25.579567559774784 31.5 19.5 35.0 2.0 37.0 78-79 24.984688235026926 31.0 17.5 35.0 2.0 36.5 80-81 24.297333512389432 31.0 11.5 34.0 2.0 36.0 82-83 23.622574596284455 30.0 7.0 34.0 2.0 35.5 84-85 22.947784082044556 30.0 2.0 34.0 2.0 35.0 86-87 22.29441364723447 29.0 2.0 34.0 2.0 35.0 88-89 21.49644570354235 29.0 2.0 34.0 2.0 35.0 90-91 20.280393949747115 27.0 2.0 33.0 2.0 35.0 92-93 18.736562399404377 25.0 2.0 32.0 2.0 34.0 94-95 18.44211024194297 25.0 2.0 32.0 2.0 34.0 96-97 17.75975246811057 23.5 2.0 32.0 2.0 34.0 98-99 16.805293773785003 19.0 2.0 32.0 2.0 34.0 100-101 15.196029891835634 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7178203128188656 1101 2 0.9962886044018973 1101 3 0.9003143732243082 1101 4 1.2556781111554685 1101 5 0.7019339730233014 1101 6 0.7766050577989745 1101 7 0.9771805896146262 1101 8 1.1181591477571118 1101 9 1.3688630285444532 1101 10-11 1.1887617097812821 1101 12-13 1.1243251439864466 1101 14-15 1.3581159680479473 1101 16-17 1.2119032579972995 1101 18-19 1.3016141805189463 1101 20-21 1.5121639430061435 1101 22-23 1.6419085091443506 1101 24-25 1.189680532981427 1101 26-27 1.2539173704270254 1101 28-29 1.390296036212753 1101 30-31 1.350797387482281 1101 32-33 1.105904569578243 1101 34-35 1.291294502396262 1101 36-37 1.207938748466546 1101 38-39 1.1815324526458184 1101 40-41 1.3920334045092844 1101 42-43 1.5061262497761092 1101 44-45 1.284020190161101 1101 46-47 1.1678914951011485 1101 48-49 1.2161989126676538 1101 50-51 1.2882383025626467 1101 52-53 0.9939378593697121 1101 54-55 1.3486291819285618 1101 56-57 1.2459820668239843 1101 58-59 1.228293621420054 1101 60-61 0.9773618952591434 1101 62-63 0.9844177850340898 1101 64-65 1.3738804682875063 1101 66-67 1.261513021961143 1101 68-69 1.2786901638478625 1101 70-71 0.9205628716035292 1101 72-73 0.8161636752135273 1101 74-75 0.8131708432229843 1101 76-77 0.23590939660774168 1101 78-79 0.8789745849445048 1101 80-81 0.7070940110806205 1101 82-83 0.19459272695791796 1101 84-85 0.1559205496518139 1101 86-87 -0.5591955824929968 1101 88-89 -0.864090350032594 1101 90-91 -3.9316985559545756 1101 92-93 -9.001611940801347 1101 94-95 -6.332777557505846 1101 96-97 -3.3707299685493037 1101 98-99 -2.816016156099554 1101 100-101 -2.2394373594000996 1102 1 0.7485308388728171 1102 2 0.6792831418783933 1102 3 0.7673332681826679 1102 4 0.8460584978105885 1102 5 0.7785510889302856 1102 6 0.7101916174041634 1102 7 0.8996704655387262 1102 8 0.6540038489103068 1102 9 1.0073514318756978 1102 10-11 0.7440473728958139 1102 12-13 0.7815866187682943 1102 14-15 0.9590146974391089 1102 16-17 1.1370000212779914 1102 18-19 1.1252587483427874 1102 20-21 0.8701893253330368 1102 22-23 0.9210966925816635 1102 24-25 1.019849781660568 1102 26-27 0.8422157296799782 1102 28-29 0.9624804486183578 1102 30-31 0.8927700151650413 1102 32-33 0.8037780161420258 1102 34-35 0.9258638684848322 1102 36-37 1.1018685316570895 1102 38-39 1.0195721131534654 1102 40-41 1.1108378183078784 1102 42-43 1.4404043310274162 1102 44-45 1.401623644784344 1102 46-47 1.1116344679477272 1102 48-49 1.1297761193181977 1102 50-51 1.173479290393857 1102 52-53 1.0353545334988432 1102 54-55 1.2668617222507415 1102 56-57 1.2607614763938457 1102 58-59 1.060772502647481 1102 60-61 1.1281049079055308 1102 62-63 1.0865378710016742 1102 64-65 1.0878653742107147 1102 66-67 1.064925606660104 1102 68-69 1.0429496884391654 1102 70-71 0.9860472352543681 1102 72-73 0.7932228177266865 1102 74-75 0.6557849940443532 1102 76-77 0.6401560360115361 1102 78-79 0.2579408844579518 1102 80-81 -0.08509254056460236 1102 82-83 -0.6806062720769397 1102 84-85 -0.8484288230037293 1102 86-87 -1.4458357822880288 1102 88-89 -2.23516758155802 1102 90-91 -5.414181665911492 1102 92-93 -9.988032744160451 1102 94-95 -8.385466283215525 1102 96-97 -5.223780375004832 1102 98-99 -3.963124948871908 1102 100-101 -3.245838909602579 1103 1 0.8462588498969268 1103 2 0.8951504178615259 1103 3 0.5732194224948017 1103 4 0.810834749728194 1103 5 0.6069004894595267 1103 6 0.5842952435924431 1103 7 0.5552028101393276 1103 8 0.7149474416655579 1103 9 0.7926656410263107 1103 10-11 0.5666835644906243 1103 12-13 0.45291178960388123 1103 14-15 0.6846263356457101 1103 16-17 0.7937236774542598 1103 18-19 0.6615200530861856 1103 20-21 0.7760103939181349 1103 22-23 0.6361899917575116 1103 24-25 0.8760681275120135 1103 26-27 0.5816849630903462 1103 28-29 0.7065374743509167 1103 30-31 0.7590581518751307 1103 32-33 0.8801716657172349 1103 34-35 0.5468400850272488 1103 36-37 0.45351989412216653 1103 38-39 0.6391963268936038 1103 40-41 0.6445945364150063 1103 42-43 0.4566584220063987 1103 44-45 0.3856591448288569 1103 46-47 0.4983517773985717 1103 48-49 0.3797145595497362 1103 50-51 0.155215403030315 1103 52-53 0.40402876151622635 1103 54-55 0.30957032894220404 1103 56-57 0.1378702597300503 1103 58-59 0.2891719602278897 1103 60-61 0.062349248742027186 1103 62-63 -0.08613641250620674 1103 64-65 -0.17844449627678216 1103 66-67 -0.29550770474677535 1103 68-69 -0.25208691794709637 1103 70-71 -0.016137703006677384 1103 72-73 -0.1600593872806364 1103 74-75 -0.2872649947997452 1103 76-77 -0.26333096360580655 1103 78-79 -0.468815245218714 1103 80-81 -0.7954703036733655 1103 82-83 -0.6697850474248241 1103 84-85 -0.8166188964477108 1103 86-87 -1.0518792752759936 1103 88-89 -1.2098824124333944 1103 90-91 -2.7279538288732077 1103 92-93 -9.145258126900364 1103 94-95 -5.846861661088459 1103 96-97 -3.4224756547289523 1103 98-99 -3.0458422028322527 1103 100-101 -2.6227615685683983 1104 1 0.7153142967206172 1104 2 0.6604409569037664 1104 3 0.4909043657560481 1104 4 0.6726891475839807 1104 5 0.7704054691647002 1104 6 0.6281070078446191 1104 7 0.8878933935313142 1104 8 0.8482396390026494 1104 9 0.8304014900829131 1104 10-11 0.46264147079696016 1104 12-13 0.4457507518198227 1104 14-15 0.5632960360920904 1104 16-17 0.790734890226986 1104 18-19 0.32524292502147745 1104 20-21 0.5891388828451625 1104 22-23 0.789962612538261 1104 24-25 0.6832204881718695 1104 26-27 0.5494571596561286 1104 28-29 0.5588422149531098 1104 30-31 0.5615952465811134 1104 32-33 0.5531433879335665 1104 34-35 0.5879980954590778 1104 36-37 0.5269558424836376 1104 38-39 0.4754001814650053 1104 40-41 0.480785375300087 1104 42-43 0.5485708601818899 1104 44-45 0.29778253353509143 1104 46-47 0.30685921562226426 1104 48-49 0.6207669983036972 1104 50-51 0.5939038074999985 1104 52-53 0.592220159593758 1104 54-55 0.42267905330853495 1104 56-57 0.6147958582105879 1104 58-59 0.39804094529359446 1104 60-61 0.3416615384422812 1104 62-63 0.2929502719646351 1104 64-65 0.5472706433767129 1104 66-67 0.2773892977888721 1104 68-69 0.11079330881508298 1104 70-71 0.24428496935475508 1104 72-73 0.21012890631468295 1104 74-75 0.14817623696624338 1104 76-77 0.11273704576548482 1104 78-79 0.010054993209379859 1104 80-81 0.13551281449942465 1104 82-83 0.17599883433489794 1104 84-85 0.22595830585461485 1104 86-87 -0.09008513522720563 1104 88-89 -0.2977493824780346 1104 90-91 -0.6728777112114024 1104 92-93 -3.9586030618701393 1104 94-95 -1.4865676994027837 1104 96-97 -0.6500906025215869 1104 98-99 -0.6211422867111231 1104 100-101 -0.39969584417314685 1105 1 1.0383497133676656 1105 2 1.020313076535004 1105 3 1.078856490660705 1105 4 1.0959543345548681 1105 5 0.8889754314032672 1105 6 1.0017888889478925 1105 7 1.1159969408461095 1105 8 1.0957926792218728 1105 9 1.3318563882400412 1105 10-11 1.2608631049913086 1105 12-13 1.1936649269537725 1105 14-15 1.191214072120836 1105 16-17 1.3992825346103288 1105 18-19 1.2387447565922898 1105 20-21 1.167105415479135 1105 22-23 1.1874808105362433 1105 24-25 1.3239788244087123 1105 26-27 1.201464354103969 1105 28-29 1.1484036738063352 1105 30-31 1.5398626465038987 1105 32-33 1.399838556165001 1105 34-35 1.5814171532124135 1105 36-37 1.38355138079595 1105 38-39 1.5063560825130935 1105 40-41 1.3126937873423117 1105 42-43 1.52093014940057 1105 44-45 1.2155073364882085 1105 46-47 1.3914401202016684 1105 48-49 1.2617546340235641 1105 50-51 1.3776409653164308 1105 52-53 1.1828880943917213 1105 54-55 1.5154088000903307 1105 56-57 1.5872271860758929 1105 58-59 1.1702811117829093 1105 60-61 1.311794692228311 1105 62-63 1.3585784207126679 1105 64-65 1.4508170164898146 1105 66-67 1.502768089234639 1105 68-69 1.1821643031367444 1105 70-71 1.2001634170867135 1105 72-73 1.241958687938702 1105 74-75 1.2120015361855891 1105 76-77 0.6963494307626128 1105 78-79 0.7645661191781343 1105 80-81 0.9420113596012669 1105 82-83 0.9671135970782068 1105 84-85 0.9405821511174857 1105 86-87 0.5163554964528458 1105 88-89 0.3499340806504243 1105 90-91 -0.7243279009602546 1105 92-93 -6.743806888864384 1105 94-95 -2.385554920782724 1105 96-97 -0.681685434572664 1105 98-99 -0.060661840730688255 1105 100-101 0.32944543632556744 1106 1 0.9678105112035595 1106 2 0.8732293242325255 1106 3 0.8427102800697561 1106 4 0.8905189329228946 1106 5 0.7901191059332007 1106 6 0.7266952326597931 1106 7 0.7793937943507672 1106 8 0.7153323619028029 1106 9 1.084988646046213 1106 10-11 0.9440545820466255 1106 12-13 1.0446919204312053 1106 14-15 1.1600939340167429 1106 16-17 1.2830580843126782 1106 18-19 1.0389798983892504 1106 20-21 0.8527945789349474 1106 22-23 1.2527843814881123 1106 24-25 1.1611431300174218 1106 26-27 1.2144021583200626 1106 28-29 0.8932659256505389 1106 30-31 1.1455368605474447 1106 32-33 1.1954031189391756 1106 34-35 1.3099273730933518 1106 36-37 1.4797081360616176 1106 38-39 1.3886405840658824 1106 40-41 1.1640233284487422 1106 42-43 1.3944613474002026 1106 44-45 1.4089809836998768 1106 46-47 1.8245654149631356 1106 48-49 1.8425515355799043 1106 50-51 1.6320740666965818 1106 52-53 1.4710902940909563 1106 54-55 1.7099608293840625 1106 56-57 1.7729169056854204 1106 58-59 1.5340746141276504 1106 60-61 1.4904568533082276 1106 62-63 1.8105028238484877 1106 64-65 1.6718152679424918 1106 66-67 1.4846663976374543 1106 68-69 1.566421481969087 1106 70-71 1.6144829296290908 1106 72-73 1.7015190817845287 1106 74-75 1.6263716587173782 1106 76-77 1.6829316658777138 1106 78-79 1.6415304541517486 1106 80-81 1.6160174016553874 1106 82-83 1.3938742685559724 1106 84-85 1.1717207535254097 1106 86-87 1.392751998468345 1106 88-89 1.2557589011240005 1106 90-91 0.4000959237756838 1106 92-93 -2.4538633718371834 1106 94-95 -0.2879204650570095 1106 96-97 0.2716237913503292 1106 98-99 0.24050485856366421 1106 100-101 0.34439095550619747 1107 1 0.7301846650708583 1107 2 0.7157498547620236 1107 3 0.80344027354122 1107 4 0.9710819333008587 1107 5 0.641504701919871 1107 6 0.7433773616499337 1107 7 0.567332323024786 1107 8 0.518308000153155 1107 9 0.6403265837158685 1107 10-11 0.5424422053157016 1107 12-13 0.5012578242899153 1107 14-15 0.5039072865920744 1107 16-17 0.8213337775401754 1107 18-19 0.5454440086184391 1107 20-21 0.6318100584387736 1107 22-23 0.44395883085758925 1107 24-25 0.46939099106266724 1107 26-27 0.4135375567325994 1107 28-29 0.3915564798889264 1107 30-31 0.46520966060241875 1107 32-33 0.4836895498913165 1107 34-35 0.32785937323079395 1107 36-37 0.5828547188881288 1107 38-39 0.35301281373292426 1107 40-41 0.38200987622732896 1107 42-43 0.1956609659962183 1107 44-45 0.3479907077833033 1107 46-47 0.46763700552081744 1107 48-49 0.6058920846641946 1107 50-51 0.3633367372116467 1107 52-53 0.5708609362927888 1107 54-55 0.5574786969578547 1107 56-57 0.32304919445290636 1107 58-59 0.4292443560786552 1107 60-61 0.15006668734595863 1107 62-63 0.23927949635647394 1107 64-65 0.4181880645613951 1107 66-67 0.25153751058113727 1107 68-69 0.5168539975173161 1107 70-71 0.4581481016044897 1107 72-73 0.6347798280993437 1107 74-75 0.4408237770556944 1107 76-77 0.32038313136237306 1107 78-79 0.2729172527635768 1107 80-81 0.06809708416234628 1107 82-83 -0.09947478480622252 1107 84-85 -0.30490966258289376 1107 86-87 -0.2547564199077641 1107 88-89 -0.4199940281272809 1107 90-91 -0.4286974995827322 1107 92-93 -4.15449775660851 1107 94-95 -1.8536288971774084 1107 96-97 -0.9724897880057526 1107 98-99 -0.5148661512978627 1107 100-101 -0.49773227921187235 1108 1 0.4056675106934122 1108 2 0.16261635140421404 1108 3 -0.048777555812925755 1108 4 -0.3255885913509786 1108 5 -0.7651849760920015 1108 6 -0.062117877323231596 1108 7 0.038949672349978925 1108 8 -0.20078373454038 1108 9 0.15583448917133325 1108 10-11 0.24409618842934577 1108 12-13 0.2316828172055594 1108 14-15 0.12272554817712944 1108 16-17 0.12769535610480176 1108 18-19 0.07224932376119142 1108 20-21 0.037355997482613645 1108 22-23 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0.31408441213222815 2311 66-67 0.321211010057187 2311 68-69 0.10117283220431617 2311 70-71 0.509598296804004 2311 72-73 0.613129788927214 2311 74-75 0.9167552307879561 2311 76-77 0.810486383806083 2311 78-79 0.6118202182755752 2311 80-81 0.6390432646318196 2311 82-83 0.7703059834964172 2311 84-85 0.5808126747866567 2311 86-87 0.9083702928398694 2311 88-89 1.1518975725087266 2311 90-91 1.8009528271822468 2311 92-93 3.049649365314327 2311 94-95 2.4497518063341985 2311 96-97 2.0871997770018 2311 98-99 1.7867129648334519 2311 100-101 2.0903306342028465 2312 1 0.23474036425219325 2312 2 -0.0928196324939492 2312 3 0.010253107056847455 2312 4 -0.10373494383281212 2312 5 0.06382414774532918 2312 6 -0.016493043706205412 2312 7 0.20247368393427223 2312 8 -0.6263458064823624 2312 9 0.01801210955193966 2312 10-11 0.009234820741120586 2312 12-13 -3.099063123173096E-4 2312 14-15 -0.4231203227016138 2312 16-17 0.08742704591046646 2312 18-19 0.1929764072793958 2312 20-21 0.08104110812224263 2312 22-23 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0.9944539145193332 2312 88-89 1.1015117224278903 2312 90-91 1.2620651532958576 2312 92-93 2.319702634495531 2312 94-95 1.5527398012416036 2312 96-97 1.1473189687474203 2312 98-99 1.3319851297335923 2312 100-101 1.0542563853998423 2313 1 -0.010987556765702067 2313 2 -0.840971223149193 2313 3 -0.34545927060758785 2313 4 -0.1015385664495625 2313 5 -0.7933191557249728 2313 6 -0.25768293037250345 2313 7 -1.2233157856390307 2313 8 -1.1063940767961142 2313 9 -1.3328818285402093 2313 10-11 -1.7941546457078914 2313 12-13 -0.4282563090804459 2313 14-15 -0.3290252348699525 2313 16-17 -1.705293353374067 2313 18-19 -1.055604383010298 2313 20-21 -1.610331549634985 2313 22-23 -1.6971071138518568 2313 24-25 -0.6547759813780019 2313 26-27 -0.9522198041567549 2313 28-29 -2.432508182222527 2313 30-31 -0.7162953094647833 2313 32-33 -0.4984011839993556 2313 34-35 -0.2684449760325336 2313 36-37 -0.22330894127628653 2313 38-39 -0.5199219721095787 2313 40-41 -0.719700061593116 2313 42-43 -0.4706259624129956 2313 44-45 -1.1170894876476076 2313 46-47 -1.1332113933803818 2313 48-49 -0.670142092605392 2313 50-51 -0.7614359983252434 2313 52-53 -0.7534991696207172 2313 54-55 -0.4641258893216502 2313 56-57 -0.9753188814187155 2313 58-59 -0.5644480220497456 2313 60-61 -1.0093093353441276 2313 62-63 -0.776996771283379 2313 64-65 -0.7847681915747486 2313 66-67 -0.5022664833232184 2313 68-69 -0.569612692950141 2313 70-71 -0.5279127358526594 2313 72-73 -0.43850304425901854 2313 74-75 -0.4371723949843336 2313 76-77 -0.36427089774731414 2313 78-79 -0.47246927863528043 2313 80-81 -0.2925542640530914 2313 82-83 -0.4578493563094099 2313 84-85 -0.33180868443664124 2313 86-87 -0.2434390628529819 2313 88-89 0.21897612502417374 2313 90-91 0.5997171696359054 2313 92-93 1.684689082878318 2313 94-95 1.2710227683818616 2313 96-97 0.8621335810618191 2313 98-99 0.9923616850452781 2313 100-101 0.9133668213167034 2314 1 -0.4808308623948072 2314 2 -0.7931745366237415 2314 3 -2.1733368019893646 2314 4 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-0.40336312145608133 2314 66-67 -0.48902116607304436 2314 68-69 -0.7002427955538479 2314 70-71 -0.551459030105871 2314 72-73 -0.14220304487194468 2314 74-75 -0.13865207344368358 2314 76-77 -0.10954332775123632 2314 78-79 -0.11371371633754634 2314 80-81 -0.05198718550057535 2314 82-83 0.26540602823996906 2314 84-85 0.18283027187403533 2314 86-87 0.36852209913093503 2314 88-89 0.3306545751810823 2314 90-91 0.9651363799428587 2314 92-93 1.8217721403952325 2314 94-95 1.1944994189898814 2314 96-97 0.857234775040439 2314 98-99 0.4425308524474545 2314 100-101 0.5320303222238323 2315 1 -7.616037566165364 2315 2 -3.391728613037703 2315 3 -1.9486859044763314 2315 4 -1.4246575980611098 2315 5 -1.7222306108885803 2315 6 -1.9993753797338059 2315 7 -3.4960135848430873 2315 8 -3.125839432884071 2315 9 -3.660573239158964 2315 10-11 -2.6669728084081115 2315 12-13 -3.185781616481325 2315 14-15 -4.992288240659505 2315 16-17 -5.25603003213816 2315 18-19 -4.214025765175155 2315 20-21 -5.140181856000304 2315 22-23 -6.578161533127858 2315 24-25 -4.701252541164646 2315 26-27 -5.138616339344956 2315 28-29 -3.4855139239834827 2315 30-31 -3.4291017146189837 2315 32-33 -2.3574775156449945 2315 34-35 -3.3305273680718166 2315 36-37 -3.8623040853142 2315 38-39 -2.8134882206239524 2315 40-41 -4.285540726133441 2315 42-43 -8.160644554630075 2315 44-45 -4.737331903393159 2315 46-47 -3.6702823146430568 2315 48-49 -6.532713426497395 2315 50-51 -7.410541357408345 2315 52-53 -7.755402601613348 2315 54-55 -5.07809641898476 2315 56-57 -4.426736159594604 2315 58-59 -4.939495418661405 2315 60-61 -3.9199197240441386 2315 62-63 -4.322857971700152 2315 64-65 -2.9654619983112767 2315 66-67 -3.5529250970798643 2315 68-69 -3.0345021592722574 2315 70-71 -2.6010823504027023 2315 72-73 -3.2865431346232477 2315 74-75 -3.514546766083072 2315 76-77 -3.7618651413292525 2315 78-79 -2.7022412810186545 2315 80-81 -2.435335560951838 2315 82-83 -2.686937095005117 2315 84-85 -2.7292579501476233 2315 86-87 -2.741490623993812 2315 88-89 -2.488716225560996 2315 90-91 -1.6733482520266527 2315 92-93 -0.8877739636962225 2315 94-95 -0.629847815767441 2315 96-97 -0.9969256240770896 2315 98-99 -1.1507302787198128 2315 100-101 -1.2849995489153567 2316 1 -4.8156098970281604 2316 2 -3.406467688665181 2316 3 -4.659522499226824 2316 4 -4.761744036065586 2316 5 -4.276468033258048 2316 6 -4.547372660602875 2316 7 -3.076288995847648 2316 8 -1.8368245238627594 2316 9 -2.3295619468457858 2316 10-11 -3.3935227790388574 2316 12-13 -4.418628652166618 2316 14-15 -3.1107956555711738 2316 16-17 -3.295729673767262 2316 18-19 -3.623107299504184 2316 20-21 -6.220236172620666 2316 22-23 -7.0730714124169225 2316 24-25 -4.785885491487708 2316 26-27 -4.845384962421932 2316 28-29 -5.451556961490333 2316 30-31 -6.761991484218839 2316 32-33 -5.366569609705806 2316 34-35 -6.8569167977803005 2316 36-37 -9.611435382379252 2316 38-39 -7.01491592936015 2316 40-41 -6.291020236953866 2316 42-43 -5.5239562515129705 2316 44-45 -4.223558053248951 2316 46-47 -6.052842422616319 2316 48-49 -3.6352401723180527 2316 50-51 -4.45718154774751 2316 52-53 -5.74678074560849 2316 54-55 -7.173134830009122 2316 56-57 -5.081357032224439 2316 58-59 -6.128014680272159 2316 60-61 -6.303433507794029 2316 62-63 -6.52980314029287 2316 64-65 -5.568722150503412 2316 66-67 -6.805431383420192 2316 68-69 -5.646205147016371 2316 70-71 -6.115693554278124 2316 72-73 -7.899326019937959 2316 74-75 -7.525088348326886 2316 76-77 -6.974941554188366 2316 78-79 -6.363654206130356 2316 80-81 -6.287247697383574 2316 82-83 -5.507806919911566 2316 84-85 -6.205723772915729 2316 86-87 -5.298605573274102 2316 88-89 -5.203374948229438 2316 90-91 -5.65586612107278 2316 92-93 -4.304022915972833 2316 94-95 -4.455281424753771 2316 96-97 -5.064856048644305 2316 98-99 -6.299623321836517 2316 100-101 -5.394066728608992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5505.0 3 2001.0 4 2002.0 5 2390.0 6 2802.0 7 3507.0 8 4367.0 9 5561.0 10 6835.0 11 8426.0 12 10255.0 13 11784.0 14 12888.0 15 14498.0 16 16368.0 17 17992.0 18 16204.0 19 14583.0 20 14911.0 21 16182.0 22 17468.0 23 19789.0 24 21969.0 25 24535.0 26 27927.0 27 30931.0 28 35009.0 29 39542.0 30 44403.0 31 50906.0 32 58390.0 33 67355.0 34 79215.0 35 92371.0 36 101113.0 37 86592.0 38 25764.0 39 378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.73573415086843 20.097210774227086 14.159308653103016 21.007746421801464 2 15.209679444030954 22.625828917546613 39.30885401068092 22.85563762774152 3 19.230453703969104 27.813101995672795 31.271503816695805 21.684940483662288 4 11.92254393993607 17.739359231093125 39.553454407436035 30.78464242153477 5 11.886340409465504 39.200381909508 34.87770791038964 14.035569770636853 6 27.998869479199506 38.7963313414163 18.135767247112675 15.069031932271526 7 25.002792034449257 35.22796343571452 23.307607083169025 16.4616374466672 8 24.947422517394056 33.506087919038585 21.450031625553446 20.096457938013916 9 26.342851778099508 16.96559425632106 22.650769101537215 34.040784864042216 10-11 23.653344863796406 27.01691271851257 28.637222805608747 20.69251961208228 12-13 26.011401023285323 25.736604341204476 28.553208563813016 19.698786071697178 14-15 22.826814262064193 26.50530009142349 28.669219935262287 21.99866571125003 16-17 19.909903187436655 29.714256911317992 29.220088347455846 21.155751553789507 18-19 19.58660994158318 28.455430797490166 32.31590251047535 19.64205675045131 20-21 20.98386031781177 26.964033655309844 31.96814567624952 20.083960350628864 22-23 20.517713838324525 27.092712207216024 31.945655418937864 20.44391853552159 24-25 20.989047769483555 27.438024455515492 31.847294671015327 19.725633103985626 26-27 20.437205912672535 26.937447436950297 33.009323282099864 19.616023368277308 28-29 19.694666150492466 28.17831544275534 32.492275927741545 19.634742479010647 30-31 20.173068822099648 27.423675482609067 32.06015382576829 20.343101869522997 32-33 20.176097805526084 27.784523683567798 32.128035534003814 19.911342976902304 34-35 20.929503642800395 27.34020942703449 31.9543079074748 19.77597902269032 36-37 20.833388953243183 27.159473899924702 32.21569450480234 19.791442642029764 38-39 20.301859555713754 27.911627228661334 31.984704332371795 19.801808883253123 40-41 20.037886521365795 27.964345935973554 32.15598727163162 19.841780271029023 42-43 19.87446468040005 28.280941262778335 31.090136376422063 20.754457680399554 44-45 20.233258776861955 27.715581478689614 31.067310900485644 20.98384884396278 46-47 20.938767196295597 28.32122230275368 30.62357058033079 20.116439920619932 48-49 20.676992883336563 28.321670022588187 30.449103395348654 20.552233698726596 50-51 19.880086483321495 28.59176786388965 31.045722220793582 20.48242343199527 52-53 20.456857586984402 29.220754492090034 29.803411866704455 20.518976054221113 54-55 20.08091952112973 28.293098596494872 30.1600042334134 21.465977648962 56-57 20.624423391255423 28.48479335034772 29.67781105724211 21.212972201154752 58-59 21.11849358071198 28.04638102441653 29.80591355589502 21.029211838976472 60-61 20.513378236420934 28.609775690539458 30.433566087869984 20.44327998516962 62-63 20.25667618088147 28.568992228135514 30.402958469315422 20.771373121667594 64-65 20.200846961692974 29.125280738936514 30.027846534653463 20.646025764717045 66-67 20.329234666084016 28.45614340359102 29.366158263195928 21.84846366712903 68-69 20.09287215595088 28.556012962951975 29.794572011017323 21.556542870079824 70-71 20.883994943684968 28.457591566612304 29.374975590970415 21.283437898732313 72-73 20.626417658254795 27.845020993828197 30.050621556762458 21.477939791154554 74-75 20.077844681561345 29.51782292065926 29.102436973750912 21.301895424028487 76-77 20.34387029413903 28.451376870981125 29.359745225576162 21.84500760930368 78-79 20.333691077336997 28.57270034076353 29.568333226174992 21.525275355724478 80-81 20.687243547385123 28.56466661654671 29.095229239456017 21.652860596612157 82-83 20.504386886870797 28.217384229365507 29.503699882432716 21.77452900133098 84-85 20.297446593669815 28.480145420092875 29.158884604943236 22.063523381294075 86-87 20.22827060366948 28.67273021274828 29.1364218748532 21.962577308729045 88-89 20.487171066298156 28.275240919639376 28.733834000383812 22.503754013678655 90-91 20.399981585917782 27.772841505737322 29.369718635793706 22.457458272551186 92-93 20.553001475537496 28.30987180740687 29.370740086527146 21.76638663052849 94-95 20.794087913696675 27.803018163185765 29.047990693864957 22.354903229252603 96-97 20.33936284509589 27.575222149453012 29.284847933233475 22.800567072217618 98-99 20.649850786438584 27.967351122861732 28.413260217368936 22.969537873330747 100-101 20.657658415425185 27.50968527195452 28.82866345720916 23.003992855411134 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5192.0 1 3534.5 2 1946.0 3 1911.0 4 1531.5 5 1513.0 6 2220.0 7 3596.0 8 5180.0 9 5470.5 10 4371.0 11 3292.0 12 2904.5 13 2795.0 14 2737.5 15 2707.5 16 2703.5 17 2772.0 18 2900.0 19 3163.0 20 3443.5 21 3815.5 22 4265.5 23 4953.0 24 5765.5 25 6651.5 26 8050.0 27 9616.0 28 10663.5 29 11890.5 30 13925.5 31 15892.5 32 18291.0 33 21297.0 34 24487.0 35 26757.5 36 29541.5 37 32894.5 38 33639.0 39 32683.0 40 31420.0 41 30317.0 42 29086.5 43 28402.0 44 29014.5 45 29958.0 46 31613.0 47 36226.0 48 44560.0 49 44120.5 50 34773.5 51 28551.5 52 25586.0 53 24356.0 54 23229.0 55 22136.5 56 21227.5 57 19859.0 58 18229.5 59 16695.5 60 14522.0 61 12087.0 62 9985.0 63 7796.0 64 5726.5 65 4215.5 66 3040.5 67 2216.0 68 1617.0 69 1098.5 70 802.5 71 567.0 72 376.5 73 289.0 74 210.5 75 144.0 76 104.0 77 73.5 78 42.0 79 18.5 80 11.0 81 8.5 82 5.0 83 3.5 84 3.5 85 5.0 86 4.0 87 4.0 88 2.5 89 0.5 90 1.0 91 1.5 92 0.5 93 0.5 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08847477777624176 2 0.09943538082664671 3 0.09667054402113916 4 0.09834919493876873 5 0.09479440476025902 6 0.0785016164420895 7 0.08995594035062081 8 0.0866973826869869 9 0.08126645324759706 10-11 0.08941284740668183 12-13 0.08679612685861217 14-15 0.09311575384262943 16-17 0.09059777746618505 18-19 0.09257266089869046 20-21 0.10368138020653331 22-23 0.11217337896630651 24-25 0.13542763138405756 26-27 0.14145102585319902 28-29 0.14135228168157374 30-31 0.14258658382688963 32-33 0.1387355611335041 34-35 0.13226781789204892 36-37 0.13720502647331242 38-39 0.1303916786311688 40-41 0.12604693507965692 42-43 0.12733060931078544 44-45 0.11656749460363103 46-47 0.1363657010144976 48-49 0.1550283494516736 50-51 0.16352034821144681 52-53 0.17245669574353376 54-55 0.16919813807989983 56-57 0.13992049119300734 58-59 0.12965109734397928 60-61 0.12644191176615802 62-63 0.123331470359962 64-65 0.10881607713104732 66-67 0.12352895870321255 68-69 0.12046788938282918 70-71 0.12930549274329084 72-73 0.15675637245511584 74-75 0.17087678899752942 76-77 0.176208974265294 78-79 0.173098532859098 80-81 0.17872695064173838 82-83 0.1784800902126752 84-85 0.15586767491048842 86-87 0.15734883748486744 88-89 0.17773950892548568 90-91 0.2588578459156448 92-93 0.5222085516402395 94-95 0.17497467211997814 96-97 0.18879885614751588 98-99 0.20598034201031284 100-101 0.2135836432254586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1012718.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.12636621321611 #Duplication Level Percentage of deduplicated Percentage of total 1 76.42864332592397 36.018042345567686 2 11.310664806225395 10.660610635462259 3 4.433408059347521 6.267912353323051 4 2.359404775804201 4.447606940390393 5 1.3077590567831094 3.081496611430545 6 0.8507358324572324 2.4055253034650876 7 0.6135002917380632 2.0238427594254023 8 0.44159193142447783 1.6648498461689083 9 0.3375397528854953 1.431631980540029 >10 1.7078105903898984 14.717318901064347 >50 0.13258019313910582 4.3218796290373485 >100 0.06872524554507146 5.6116707069709255 >500 0.0025453794454949733 0.8622728151010882 >1k 0.004242299075824956 3.7063936283698293 >5k 8.484598151649913E-4 2.77894554368308 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9667 0.9545599071014833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6620 0.6536864161592862 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6506 0.6424295805940055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5007 0.494412067327726 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3967 0.39171812883744533 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3914 0.38648468774130607 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3825 0.37769645646665706 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2814 0.2778660989535093 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2255 0.22266810701498346 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1783 0.1760608580078561 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1693 0.1671738825615818 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1643 0.16223667398031832 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1637 0.1616442089505667 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1588 0.15680574454092847 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1477 0.14584514149052352 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1337 0.13202095746298576 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1301 0.12846616728447602 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1299 0.12826867894122548 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1231 0.12155407527070716 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1150 0.1135557973690603 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1055 0.10417510106465964 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1049 0.10358263603490803 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1037 0.1023977059754048 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1023 0.10101528757265102 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.874417162526982E-5 0.0 10-11 0.0 0.0 0.0 9.874417162526982E-5 0.0 12-13 0.0 0.0 0.0 1.4811625743790474E-4 0.0 14-15 0.0 0.0 0.0 2.4686042906317453E-4 0.0 16-17 0.0 0.0 0.0 5.430929439389841E-4 0.0 18-19 0.0 0.0 0.0 9.380696304400633E-4 0.0 20-21 0.0 0.0 0.0 0.0013330463169411427 0.0 22-23 0.0 0.0 0.0 0.0021229996899433013 0.0 24-25 0.0 0.0 0.0 0.003307929749446539 0.0 26-27 0.0 0.0 0.0 0.005381557353577204 0.0 28-29 0.0 0.0 0.0 0.01288611439709771 0.0 30-31 0.0 0.0 0.0 0.03189436743496215 0.0 32-33 0.0 0.0 0.0 0.06936778056675205 0.0 34-35 0.0 0.0 0.0 0.12984858568722982 0.0 36-37 0.0 0.0 0.0 0.21778027051953258 0.0 38-39 0.0 0.0 0.0 0.3415067175659957 0.0 40-41 0.0 0.0 0.0 0.478859860296746 0.0 42-43 0.0 0.0 0.0 0.6050549116338408 0.0 44-45 0.0 0.0 0.0 0.7594907960557629 0.0 46-47 0.0 0.0 0.0 0.9477959313451524 0.0 48-49 0.0 0.0 0.0 1.1362985549777924 0.0 50-51 0.0 0.0 0.0 1.337588548835905 0.0 52-53 0.0 0.0 0.0 1.5355212408587584 0.0 54-55 0.0 0.0 0.0 1.7484630469686526 0.0 56-57 0.0 0.0 0.0 2.0075183812275483 0.0 58-59 0.0 0.0 0.0 2.2741770167015893 0.0 60-61 0.0 0.0 0.0 2.572779391696405 0.0 62-63 0.0 0.0 0.0 2.8647659071923277 0.0 64-65 0.0 0.0 0.0 3.160405957038386 0.0 66-67 0.0 0.0 0.0 3.5034925813503857 0.0 68-69 0.0 0.0 0.0 3.8821764795332956 0.0 70-71 0.0 0.0 0.0 4.300851767224439 0.0 72-73 0.0 0.0 0.0 4.719576427001396 0.0 74-75 0.0 0.0 0.0 5.155334456383712 0.0 76-77 0.0 0.0 0.0 5.598597042809548 0.0 78-79 0.0 0.0 0.0 6.141591242576907 0.0 80-81 0.0 0.0 0.0 6.711888205798653 0.0 82-83 0.0 0.0 0.0 7.249500848212435 0.0 84-85 0.0 0.0 0.0 7.801332651340255 0.0 86-87 0.0 0.0 0.0 8.37686305565814 0.0 88-89 0.0 0.0 0.0 8.990804942738254 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4855 0.0 29.622307 1 TACGCTT 65 0.00586536 29.208693 5 TACATGG 5135 0.0 27.175175 2 ACATGGG 4945 0.0 26.779558 3 CATGGGA 3385 0.0 23.697008 4 GAGTACT 6165 0.0 23.133106 12-13 GTCGGGA 165 2.4454057E-6 23.012909 2 CGCCGTA 95 3.287587E-7 22.501352 94-95 AGTACTT 6460 0.0 22.150187 12-13 GAGTACG 150 3.0504913E-5 22.149925 1 CGTATAG 195 4.2512147E-7 21.906519 1 ACGCGCG 55 0.002877522 21.591675 82-83 CATGGGG 1590 0.0 21.493189 4 ATGGGAT 1355 0.0 21.017324 5 GTACTTT 6790 0.0 20.829544 14-15 CATATAG 325 2.1827873E-11 20.446085 3 TAGGACA 445 0.0 19.198973 4 GTATATG 325 4.9294613E-10 18.985651 1 GTCCAAG 400 1.8189894E-12 18.98565 1 AGAGTAC 10110 0.0 18.941994 10-11 >>END_MODULE