##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939701_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012718 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.528989313905747 31.0 31.0 34.0 30.0 34.0 2 31.731232189020044 31.0 31.0 34.0 30.0 34.0 3 31.755407724559056 31.0 31.0 34.0 30.0 34.0 4 35.55058663912362 37.0 35.0 37.0 33.0 37.0 5 35.29213858152023 37.0 35.0 37.0 33.0 37.0 6 35.336924988002586 37.0 35.0 37.0 32.0 37.0 7 35.26503824361767 37.0 35.0 37.0 32.0 37.0 8 35.24561230273383 37.0 35.0 37.0 32.0 37.0 9 36.821361919112725 39.0 37.0 39.0 32.0 39.0 10-11 36.71327605513085 39.0 35.0 39.0 32.0 39.0 12-13 36.66680852912657 39.0 35.0 39.0 32.0 39.0 14-15 37.63724551158368 39.0 36.5 41.0 32.0 41.0 16-17 37.621385716458086 39.0 36.0 41.0 32.0 41.0 18-19 37.6691285234389 39.0 36.5 41.0 32.0 41.0 20-21 37.62651202012801 39.0 36.0 41.0 32.0 41.0 22-23 37.472185248015734 39.0 36.0 41.0 32.0 41.0 24-25 37.31641681099774 39.0 36.0 41.0 31.5 41.0 26-27 37.02319303103134 39.0 36.0 40.5 31.0 41.0 28-29 36.82748948868293 39.0 35.0 40.0 30.5 41.0 30-31 36.53898814872452 38.0 35.0 40.0 30.0 41.0 32-33 36.26188287361339 38.0 35.0 40.0 30.0 41.0 34-35 36.16088190394562 38.0 35.0 40.0 30.0 41.0 36-37 35.96346120045264 38.0 35.0 40.0 30.0 41.0 38-39 35.90457067021619 38.0 35.0 40.0 30.0 41.0 40-41 35.800532823550085 38.0 35.0 40.0 29.0 41.0 42-43 35.54810371692811 38.0 35.0 40.0 27.5 41.0 44-45 35.25541019316335 38.0 34.0 40.0 27.0 41.0 46-47 34.838793721450585 38.0 34.0 40.0 25.5 41.0 48-49 34.42714605645402 38.0 33.0 40.0 24.0 41.0 50-51 34.138206292373596 38.0 33.0 40.0 23.0 41.0 52-53 33.90210947173843 38.0 33.0 40.0 23.0 41.0 54-55 33.623934797248594 37.5 33.0 40.0 21.5 41.0 56-57 31.693267029913557 36.0 30.0 39.5 13.0 41.0 58-59 26.940997395128754 32.5 16.5 38.0 2.0 40.0 60-61 27.62471783852958 32.5 18.5 38.0 2.0 40.0 62-63 30.498580552532886 34.5 29.0 38.0 2.0 40.0 64-65 30.706910512107022 35.0 29.5 38.5 2.0 40.0 66-67 24.25492782788496 26.5 16.5 35.5 2.0 38.5 68-69 17.00315487628343 13.0 7.0 28.0 2.0 35.0 70-71 15.923581391858345 12.5 7.0 26.5 2.0 33.5 72-73 17.06664145398818 13.0 7.0 28.5 2.0 34.0 74-75 18.05186636358789 17.0 7.5 29.5 2.0 34.5 76-77 19.163354951723974 21.0 7.0 30.0 2.0 34.0 78-79 22.172597899908958 27.5 9.0 33.5 2.0 35.5 80-81 25.57694935806414 31.5 21.5 34.5 2.0 36.0 82-83 26.750302157165173 33.0 25.5 35.0 2.0 36.0 84-85 26.77230581464929 33.0 25.5 35.0 2.0 36.0 86-87 26.554316206485915 33.0 25.0 35.0 2.0 35.5 88-89 26.13444413943467 33.0 24.5 35.0 2.0 35.0 90-91 24.883474965390167 32.5 11.0 35.0 2.0 35.0 92-93 22.62807020315626 31.5 2.0 34.5 2.0 35.0 94-95 15.617320912633133 4.5 2.0 32.0 2.0 35.0 96-97 13.151151159552807 2.0 2.0 30.0 2.0 34.0 98-99 12.8709339618729 2.0 2.0 29.5 2.0 34.0 100-101 12.596568837524366 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.31543277729340957 1101 2 0.3367468506562652 1101 3 0.5915356685621269 1101 4 0.35733803541749154 1101 5 0.5105702690445284 1101 6 0.36469827446449443 1101 7 0.40216715413226467 1101 8 0.5797360633393964 1101 9 0.5081252221702357 1101 10-11 0.4883829651719154 1101 12-13 0.47690134976147647 1101 14-15 0.9981240586003111 1101 16-17 0.8378151818624318 1101 18-19 0.7979517705067494 1101 20-21 0.4976427875566287 1101 22-23 0.68403917843073 1101 24-25 0.5371124744380182 1101 26-27 0.5772412812309042 1101 28-29 0.5882195950378701 1101 30-31 0.41852708991380894 1101 32-33 0.7808569497420166 1101 34-35 0.7642476236978055 1101 36-37 0.2261890007122318 1101 38-39 0.514833266263544 1101 40-41 0.6074248321483822 1101 42-43 0.5982925421964111 1101 44-45 0.2312148640878462 1101 46-47 0.7349237560404731 1101 48-49 0.6893289935550229 1101 50-51 0.6554532032483991 1101 52-53 0.4291630635948067 1101 54-55 0.38795503837172873 1101 56-57 -1.9256163089030593 1101 58-59 -9.793386430142363 1101 60-61 -10.008262715755013 1101 62-63 -3.2465055649170544 1101 64-65 -4.584877260081161 1101 66-67 -9.431142294583964 1101 68-69 -4.35494440492289 1101 70-71 -4.070455985233444 1101 72-73 -4.093256037611912 1101 74-75 -6.070028630288462 1101 76-77 -7.26329595741602 1101 78-79 -11.143398903794878 1101 80-81 -7.659579386127962 1101 82-83 -3.751268841056188 1101 84-85 -3.01809150888079 1101 86-87 -2.7537099844473403 1101 88-89 -2.6962164141269582 1101 90-91 -9.544274398560141 1101 92-93 -16.439215500537728 1101 94-95 -11.020519280733232 1101 96-97 -9.052827113000928 1101 98-99 -9.273437521964368 1101 100-101 -9.021287726049984 1102 1 0.3310989763123473 1102 2 0.3832081503189606 1102 3 0.3088053023044033 1102 4 0.32210525629646725 1102 5 0.2692118021330927 1102 6 0.2394905692218643 1102 7 0.45491184832049925 1102 8 0.2621037538560955 1102 9 0.4217671035071149 1102 10-11 0.4958802538100642 1102 12-13 0.2923745197166028 1102 14-15 0.4635069327433641 1102 16-17 0.4667479002887447 1102 18-19 0.4610202858744472 1102 20-21 0.6236626576102609 1102 22-23 0.741838448104275 1102 24-25 0.4536124794129961 1102 26-27 0.434285349567169 1102 28-29 0.5003714711012677 1102 30-31 0.4753127082535613 1102 32-33 0.5647303167061892 1102 34-35 0.5538036568510449 1102 36-37 0.41968446850921026 1102 38-39 0.640141714767573 1102 40-41 0.5714184442793595 1102 42-43 0.6357416730681322 1102 44-45 0.7724611452933772 1102 46-47 0.7026063127800697 1102 48-49 0.7330341565849778 1102 50-51 0.6852595274377293 1102 52-53 0.40085047591086465 1102 54-55 0.2051918372732544 1102 56-57 -2.6148133975471772 1102 58-59 -9.780677854278956 1102 60-61 -10.05799754954107 1102 62-63 -4.054068523236705 1102 64-65 -4.774204941841905 1102 66-67 -6.056170900695221 1102 68-69 -3.9664572453356115 1102 70-71 -4.049414324785495 1102 72-73 -4.334508786286083 1102 74-75 -4.76330768663504 1102 76-77 -6.848395407183689 1102 78-79 -9.41713451367717 1102 80-81 -5.487360646388847 1102 82-83 -2.904056568785709 1102 84-85 -2.467784552869528 1102 86-87 -2.3946697070426843 1102 88-89 -2.543512017243284 1102 90-91 -8.50462010963344 1102 92-93 -12.00118255624751 1102 94-95 -9.860052677746257 1102 96-97 -8.782076488641458 1102 98-99 -8.895187618976397 1102 100-101 -8.898545121351617 1103 1 0.12456797620020765 1103 2 0.10400425396133173 1103 3 0.08315204622617145 1103 4 0.08410536496536025 1103 5 0.21989346818897815 1103 6 0.043818365451514296 1103 7 0.22823086202318166 1103 8 0.20528033039309435 1103 9 0.4617214163324732 1103 10-11 0.30011961206248117 1103 12-13 0.30565986918534094 1103 14-15 0.29221817754547175 1103 16-17 0.2099378870288291 1103 18-19 0.2894800893687304 1103 20-21 0.2486284095700526 1103 22-23 0.3925327151268547 1103 24-25 0.41554888961723435 1103 26-27 0.39461369145335823 1103 28-29 0.40870695276455393 1103 30-31 0.38679727553752485 1103 32-33 0.3122069442250819 1103 34-35 0.34806050617007145 1103 36-37 0.02510831763055421 1103 38-39 0.09628341437208832 1103 40-41 0.3710856169114436 1103 42-43 0.1293932135652156 1103 44-45 0.2192844878327378 1103 46-47 0.3524260264722372 1103 48-49 0.08426529415169881 1103 50-51 0.07429587718044672 1103 52-53 9.044635387951416E-4 1103 54-55 0.04133649637086734 1103 56-57 -2.724382849104554 1103 58-59 -10.12647150131085 1103 60-61 -10.367691161082167 1103 62-63 -4.308197651931881 1103 64-65 -5.208583962735233 1103 66-67 -6.1359118988073895 1103 68-69 -3.735752042061211 1103 70-71 -3.9989798226450652 1103 72-73 -4.231138085498927 1103 74-75 -5.171630451726433 1103 76-77 -6.535686234026754 1103 78-79 -9.271033241210745 1103 80-81 -4.962013628482762 1103 82-83 -3.124444008049629 1103 84-85 -2.533479519303558 1103 86-87 -2.491454957668978 1103 88-89 -2.439226879332061 1103 90-91 -5.0327959867589875 1103 92-93 -9.471382426076055 1103 94-95 -8.338181330148013 1103 96-97 -7.828421456867368 1103 98-99 -8.217744042983899 1103 100-101 -8.116671322923153 1104 1 0.11412168277843193 1104 2 0.1431197657670431 1104 3 0.1009083347309101 1104 4 0.14318983194890222 1104 5 0.21083165814086868 1104 6 -0.027231855815152528 1104 7 0.04384004434884048 1104 8 0.11028124631176439 1104 9 0.09381520640391017 1104 10-11 0.16722704450143056 1104 12-13 0.13518282017886918 1104 14-15 0.13563754295845598 1104 16-17 0.018690212980182253 1104 18-19 0.1802789632708297 1104 20-21 9.765357932920438E-4 1104 22-23 0.06192898010694137 1104 24-25 0.19006658612999416 1104 26-27 0.3869956584610037 1104 28-29 0.22473938592659692 1104 30-31 0.21630528259490944 1104 32-33 0.25121833054028997 1104 34-35 0.04611634828497557 1104 36-37 -0.038550439998083164 1104 38-39 -0.16931950114165062 1104 40-41 -0.306042929863068 1104 42-43 -0.2715771781587435 1104 44-45 -0.1772263441106503 1104 46-47 -0.25666615351060074 1104 48-49 -0.42537932940572887 1104 50-51 -0.3250567129411266 1104 52-53 -0.4423206307044225 1104 54-55 -0.5498094624995744 1104 56-57 -3.1564332535549546 1104 58-59 -10.492253744058218 1104 60-61 -10.583721183931932 1104 62-63 -4.928361842404396 1104 64-65 -6.03224262395138 1104 66-67 -7.064371902085416 1104 68-69 -4.546254254727884 1104 70-71 -4.133210113521553 1104 72-73 -4.35234449500134 1104 74-75 -4.949974277952558 1104 76-77 -6.559678000400005 1104 78-79 -8.929698795920011 1104 80-81 -5.512516901686833 1104 82-83 -3.4239204882224 1104 84-85 -2.8416331236845416 1104 86-87 -2.7724159009687916 1104 88-89 -2.7338191027005188 1104 90-91 -4.572825971043752 1104 92-93 -6.364709235497486 1104 94-95 -7.755111845665603 1104 96-97 -7.71461036750263 1104 98-99 -7.783469884423916 1104 100-101 -7.632963105686528 1105 1 0.4228755554442394 1105 2 0.36436504558774985 1105 3 0.44147598043260317 1105 4 0.24821652862382138 1105 5 0.40333681384792897 1105 6 0.33423453085816845 1105 7 0.48677507773145834 1105 8 0.3291205295036903 1105 9 0.5167696384286202 1105 10-11 0.6052432037586328 1105 12-13 0.5087681789124971 1105 14-15 0.7085858540524086 1105 16-17 0.5243374778658065 1105 18-19 0.6881453009767071 1105 20-21 0.5226361555422372 1105 22-23 0.5293764578126243 1105 24-25 0.6255213712853234 1105 26-27 0.7658101754254005 1105 28-29 0.8007839463025093 1105 30-31 0.7810298877948796 1105 32-33 0.6464270542791439 1105 34-35 0.6889680428411111 1105 36-37 0.7456985217978911 1105 38-39 0.6248034636488669 1105 40-41 0.5474817845719642 1105 42-43 0.6228993880932734 1105 44-45 0.8653753339660639 1105 46-47 0.7873664098410273 1105 48-49 0.831643732378005 1105 50-51 0.8598777930997628 1105 52-53 0.9321226000170029 1105 54-55 1.012697011561805 1105 56-57 2.1778317168291608 1105 58-59 -8.420851674241941 1105 60-61 -9.435787233483218 1105 62-63 -3.2802998232505907 1105 64-65 -4.195209109501274 1105 66-67 -5.6123185652364675 1105 68-69 -3.533821231835603 1105 70-71 -3.6089900613523866 1105 72-73 -1.9133471034595253 1105 74-75 -3.924009289676494 1105 76-77 -5.617522208586987 1105 78-79 -8.316397071700901 1105 80-81 -3.685369632092794 1105 82-83 -1.1520052019040925 1105 84-85 -0.7177690271810029 1105 86-87 -0.6377398865422279 1105 88-89 -0.8466118479850486 1105 90-91 -3.6111302848874125 1105 92-93 -6.039026280877124 1105 94-95 -7.640020926301803 1105 96-97 -7.7771031871800105 1105 98-99 -8.434365423677916 1105 100-101 -8.17365411168504 1106 1 0.18363045627090813 1106 2 0.1988381232979961 1106 3 0.31789417244356954 1106 4 0.09543887443576438 1106 5 0.1400692104700738 1106 6 0.137732070434069 1106 7 0.08944661778787122 1106 8 -0.06114255345665498 1106 9 0.06350434865028376 1106 10-11 0.13516705518994598 1106 12-13 0.25065556445606063 1106 14-15 0.18938297807023474 1106 16-17 0.3616625119024164 1106 18-19 0.3770557068354563 1106 20-21 0.1944298180592554 1106 22-23 0.17229684329389983 1106 24-25 0.2511748519178312 1106 26-27 0.2670103551742855 1106 28-29 0.2837912252425312 1106 30-31 0.3481759960535413 1106 32-33 0.3690959915361063 1106 34-35 0.49479141731499965 1106 36-37 0.45045485972580224 1106 38-39 0.2107920602728015 1106 40-41 0.43481838660615324 1106 42-43 0.2451158386756802 1106 44-45 0.2416633860042552 1106 46-47 0.46946170789493635 1106 48-49 0.505190947605044 1106 50-51 0.4244144280582489 1106 52-53 0.5679392339076728 1106 54-55 0.356521258084733 1106 56-57 1.7024025886112497 1106 58-59 -8.14793535980597 1106 60-61 -9.765653133695892 1106 62-63 -3.6376586269416755 1106 64-65 -4.995643354333492 1106 66-67 -6.426280693392087 1106 68-69 -3.683656276639347 1106 70-71 -3.7936436665633266 1106 72-73 -4.454749411719966 1106 74-75 -5.05335118184496 1106 76-77 -6.3845566411767045 1106 78-79 -9.058281676475815 1106 80-81 -4.430677363074562 1106 82-83 -2.145774055515531 1106 84-85 -1.712864307437993 1106 86-87 -1.6657110822282526 1106 88-89 -1.8534147204078302 1106 90-91 -4.2067753787216695 1106 92-93 -5.930766508143783 1106 94-95 -6.311970311863162 1106 96-97 -6.804684519101454 1106 98-99 -6.075234158049996 1106 100-101 -6.65247744611585 1107 1 0.08730447613140058 1107 2 0.069100639189827 1107 3 0.016413225462159176 1107 4 0.03058301765123872 1107 5 -0.15436778915196214 1107 6 -0.052952738692141565 1107 7 0.12558979890322064 1107 8 0.25137247935783336 1107 9 0.3452278823066024 1107 10-11 0.2543966019720685 1107 12-13 0.1387663262331884 1107 14-15 0.06819272277009958 1107 16-17 0.11442854976792916 1107 18-19 0.07839448634272372 1107 20-21 0.0619134108160182 1107 22-23 0.0653534010370933 1107 24-25 0.24460551198723834 1107 26-27 0.2013169558683714 1107 28-29 0.3261193357466041 1107 30-31 0.11076275686857429 1107 32-33 0.14586943413652875 1107 34-35 -0.001033755347620513 1107 36-37 0.02868347876850663 1107 38-39 0.09439727279384869 1107 40-41 0.20790019947767746 1107 42-43 0.1149424029473991 1107 44-45 -0.03251796986970845 1107 46-47 0.04259205528180132 1107 48-49 -0.2824490723241695 1107 50-51 -0.22686937429889298 1107 52-53 -0.146499569652093 1107 54-55 -0.34673957413187395 1107 56-57 0.9267437218277621 1107 58-59 -9.228779688569329 1107 60-61 -10.177114151460383 1107 62-63 -3.941682267116228 1107 64-65 -5.392449525518597 1107 66-67 -7.13689471943751 1107 68-69 -3.864511772636323 1107 70-71 -4.058871907638128 1107 72-73 -2.7469701449777055 1107 74-75 -4.423048893421086 1107 76-77 -6.588442408966966 1107 78-79 -7.579572436878522 1107 80-81 -2.5467156580956924 1107 82-83 -1.4036984962948367 1107 84-85 -1.299734647265499 1107 86-87 -1.2543101957217075 1107 88-89 -1.0165851839339304 1107 90-91 -0.8749668022862522 1107 92-93 -3.799066167972665 1107 94-95 -5.806111809303289 1107 96-97 -6.522048278851308 1107 98-99 -6.07727647327523 1107 100-101 -6.73613935382296 1108 1 -0.29080879841033536 1108 2 -0.32820184391868423 1108 3 -0.3289993570321492 1108 4 -0.1923351213659643 1108 5 -0.28353603486122836 1108 6 -0.46021855387075306 1108 7 -0.1789281407278409 1108 8 -0.1387969988582043 1108 9 -0.40716628769738605 1108 10-11 -0.23902433835207404 1108 12-13 -0.3323141486062511 1108 14-15 -0.49786247031693875 1108 16-17 -0.3599824246635279 1108 18-19 -0.30250771885429373 1108 20-21 -0.3375088302978142 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58-59 6.662556494247166 2311 60-61 5.709350307683248 2311 62-63 1.8943654303228747 2311 64-65 1.123626749393722 2311 66-67 0.056016447429147576 2311 68-69 0.8026424796057441 2311 70-71 0.6911499923919528 2311 72-73 -2.361656057225524 2311 74-75 -0.11343411908230294 2311 76-77 2.9534287957165013 2311 78-79 5.381289730117057 2311 80-81 2.476671094782713 2311 82-83 1.2062648604095507 2311 84-85 0.817237132273096 2311 86-87 0.7669927486340349 2311 88-89 0.9622974021538475 2311 90-91 1.9932375770586326 2311 92-93 3.681848575623434 2311 94-95 5.631781358217891 2311 96-97 10.996951856680951 2311 98-99 11.845788721048281 2311 100-101 11.117169286193436 2312 1 -0.16948515629708893 2312 2 -0.27777550014927144 2312 3 -0.27650381407912406 2312 4 -0.24862217939227804 2312 5 -0.22608732115288888 2312 6 -0.24122790358558177 2312 7 -0.35385735233472104 2312 8 -0.35137353502683766 2312 9 -0.05432466815575765 2312 10-11 -0.40846674362469315 2312 12-13 -0.23946276793007826 2312 14-15 -0.32545017935445486 2312 16-17 -0.2634044686935013 2312 18-19 -0.4471374814409117 2312 20-21 -0.4130882464044916 2312 22-23 -0.41122593010870645 2312 24-25 -0.3489702727066728 2312 26-27 -0.5282462008052562 2312 28-29 -0.5963277160581129 2312 30-31 -0.5194409155320727 2312 32-33 -0.4076140433749913 2312 34-35 -0.4109547059292993 2312 36-37 -0.5778708283475922 2312 38-39 -0.6064189083072122 2312 40-41 -0.5835667721175639 2312 42-43 -0.48570639888216505 2312 44-45 -0.48808208773122885 2312 46-47 -0.31174820523326474 2312 48-49 -0.5000893560403199 2312 50-51 -0.45005886089197844 2312 52-53 -0.45816391621004726 2312 54-55 -0.4374931122976662 2312 56-57 1.1119016549423364 2312 58-59 6.1250501480287305 2312 60-61 5.185739250813324 2312 62-63 1.7877391972038161 2312 64-65 0.8360364378236902 2312 66-67 2.532706884751942 2312 68-69 5.3084755330545725 2312 70-71 2.463769867576481 2312 72-73 4.063700697884645 2312 74-75 2.804441909005085 2312 76-77 3.9719699074958648 2312 78-79 5.763139935929804 2312 80-81 2.8152561028668224 2312 82-83 1.4335715643594362 2312 84-85 0.9702149732310019 2312 86-87 1.0205903710477067 2312 88-89 1.08438263284377 2312 90-91 2.2088777835743407 2312 92-93 4.159107243581474 2312 94-95 10.428404779474013 2312 96-97 13.003965111490409 2312 98-99 12.794999056206722 2312 100-101 11.781866347015592 2313 1 -0.3452781406990617 2313 2 -0.34346452372986747 2313 3 -0.35725583120024496 2313 4 -0.3748154637262999 2313 5 -0.4116749614531301 2313 6 -0.4268788108019166 2313 7 -0.41635413040843616 2313 8 -0.48883614115514007 2313 9 -0.46152459942133106 2313 10-11 -0.4781833703264553 2313 12-13 -0.3501216811450476 2313 14-15 -0.39648955324631174 2313 16-17 -0.6775145531275015 2313 18-19 -0.4769584595005725 2313 20-21 -0.4676024580284235 2313 22-23 -0.4480886721437187 2313 24-25 -0.42754153390530547 2313 26-27 -0.22249242626980248 2313 28-29 -0.5467522292543379 2313 30-31 -0.7803407897793164 2313 32-33 -0.8022679889654398 2313 34-35 -0.7466020983169628 2313 36-37 -0.7558937762734601 2313 38-39 -0.7312789009651297 2313 40-41 -0.8035274841437428 2313 42-43 -0.7039249274967787 2313 44-45 -0.652071886917355 2313 46-47 -0.7946626230604679 2313 48-49 -0.9001807415857854 2313 50-51 -1.0419587429499515 2313 52-53 -0.8825236315870342 2313 54-55 -1.022669127106802 2313 56-57 0.6075561550946489 2313 58-59 5.447640342437811 2313 60-61 4.476781905211936 2313 62-63 1.1194316262628554 2313 64-65 0.4135649665164074 2313 66-67 2.503059695443273 2313 68-69 4.940330034769026 2313 70-71 5.6840890921271665 2313 72-73 4.659526643568828 2313 74-75 2.568825369002429 2313 76-77 3.403119881329914 2313 78-79 5.208738979896406 2313 80-81 1.9228317302637414 2313 82-83 0.8726373228985196 2313 84-85 0.7267693476305546 2313 86-87 0.4245390857920235 2313 88-89 0.7729770138043328 2313 90-91 1.652549139282815 2313 92-93 3.567550252587246 2313 94-95 10.095594244360877 2313 96-97 12.037110726936909 2313 98-99 11.65558572016567 2313 100-101 10.872244308258749 2314 1 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6.538210792079862 2314 60-61 5.655799784244987 2314 62-63 2.054309071835089 2314 64-65 1.2314288296624305 2314 66-67 3.2921149169544996 2314 68-69 10.592938874991642 2314 70-71 7.952247006219551 2314 72-73 6.2587404901658665 2314 74-75 6.381560579113245 2314 76-77 6.778203775489963 2314 78-79 6.891757346205122 2314 80-81 3.4590437442139184 2314 82-83 2.219350817063468 2314 84-85 1.7489691214610907 2314 86-87 1.6627563617065064 2314 88-89 1.862711203394408 2314 90-91 2.8694542339184927 2314 92-93 5.023791978094749 2314 94-95 11.409268379523178 2314 96-97 13.072306434007618 2314 98-99 12.870225672480077 2314 100-101 11.790911793180788 2315 1 -0.46857783704211187 2315 2 -0.484043670495268 2315 3 -0.43449876631706985 2315 4 -0.4470295785169114 2315 5 -0.5762806057124834 2315 6 -0.5792139104224248 2315 7 -0.6051224876339134 2315 8 -0.6635296961718495 2315 9 -1.1307790945319098 2315 10-11 -0.8802607757642491 2315 12-13 -0.9081498705785549 2315 14-15 -1.1329930693579726 2315 16-17 -1.0321746357808195 2315 18-19 -0.957270544282359 2315 20-21 -1.069580051778125 2315 22-23 -1.0800464150772413 2315 24-25 -1.2757250089770835 2315 26-27 -1.1251831996231871 2315 28-29 -1.029456050616112 2315 30-31 -1.1740166479367886 2315 32-33 -1.2723420073378051 2315 34-35 -1.190032051701003 2315 36-37 -1.1276903229324304 2315 38-39 -1.052189011498804 2315 40-41 -1.153289784161906 2315 42-43 -1.0517969391205 2315 44-45 -1.2485785574925785 2315 46-47 -1.0725409914062283 2315 48-49 -1.095703713409094 2315 50-51 -1.0037001183597951 2315 52-53 -1.3471259302032124 2315 54-55 -0.9661942720050192 2315 56-57 0.518133883944607 2315 58-59 5.584020308726469 2315 60-61 4.747102306306758 2315 62-63 1.2601322517388134 2315 64-65 0.7281916405328666 2315 66-67 6.8162465480214856 2315 68-69 13.22480967573372 2315 70-71 7.699577030286939 2315 72-73 10.705229722516446 2315 74-75 6.425975566076744 2315 76-77 5.879206896729432 2315 78-79 6.178602700697699 2315 80-81 2.7032133566286802 2315 82-83 1.1558480092014563 2315 84-85 0.7959146313888894 2315 86-87 0.7920026262472213 2315 88-89 0.733629462018559 2315 90-91 1.7851556980179701 2315 92-93 3.8485325015604523 2315 94-95 10.321496054513153 2315 96-97 12.158090379053746 2315 98-99 11.60442048044237 2315 100-101 10.644754114179582 2316 1 -0.4975643007859354 2316 2 -0.3197243442061577 2316 3 -0.5243059852137115 2316 4 -0.38964238615159275 2316 5 -0.5924039961783691 2316 6 -0.5409447824582685 2316 7 -0.5497383443911517 2316 8 -0.4757413659954395 2316 9 -0.7149368569247514 2316 10-11 -0.5936886296967501 2316 12-13 -0.5644000768233113 2316 14-15 -3.2524599348995906 2316 16-17 -1.13932703910978 2316 18-19 -0.8991977943302061 2316 20-21 -0.7749024865882816 2316 22-23 -0.6089536808326912 2316 24-25 -0.6252455311558194 2316 26-27 -0.64664502949271 2316 28-29 -0.6425144789320356 2316 30-31 -0.7838881821998172 2316 32-33 -0.7259732351046182 2316 34-35 -0.6404227617769678 2316 36-37 -0.6163534608887318 2316 38-39 -0.7786342097907735 2316 40-41 -0.5752616160773769 2316 42-43 -0.6155975185857798 2316 44-45 -0.9317701044272866 2316 46-47 -0.927192058269128 2316 48-49 -0.8221711861352858 2316 50-51 -0.6452409482227139 2316 52-53 -0.6779128282160016 2316 54-55 -0.5836968624392185 2316 56-57 0.8825428029580706 2316 58-59 5.900245501606573 2316 60-61 4.789472405106249 2316 62-63 1.4292471812196048 2316 64-65 0.5937349057339283 2316 66-67 6.915324056452771 2316 68-69 13.74065669821935 2316 70-71 14.4642633072555 2316 72-73 13.018591413129503 2316 74-75 11.639398470134982 2316 76-77 9.704223066318725 2316 78-79 7.251837740527687 2316 80-81 3.295970700592143 2316 82-83 1.5528238010163449 2316 84-85 1.1666379400168516 2316 86-87 0.7366902561814186 2316 88-89 1.0198221177251021 2316 90-91 1.841276547548393 2316 92-93 3.8673420237985887 2316 94-95 10.505088541263985 2316 96-97 12.23326329466169 2316 98-99 12.162881795524969 2316 100-101 11.136730367982734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 23.0 5 85.0 6 183.0 7 373.0 8 758.0 9 1321.0 10 2182.0 11 3381.0 12 5000.0 13 6745.0 14 8153.0 15 10835.0 16 14343.0 17 18769.0 18 19778.0 19 18046.0 20 18209.0 21 18218.0 22 17988.0 23 15783.0 24 16098.0 25 18493.0 26 25334.0 27 37203.0 28 51791.0 29 68166.0 30 84614.0 31 93565.0 32 94043.0 33 87284.0 34 79148.0 35 70701.0 36 56028.0 37 35772.0 38 13416.0 39 880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.557656960958894 21.097939055621893 14.957871480113027 21.38653250330618 2 15.279567630767893 23.327141676368903 38.589329217412086 22.803961475451118 3 18.873269755252696 27.725980973973012 31.799968006888395 21.6007812638859 4 11.938565326181621 18.385276059080613 39.11207265991125 30.564085954826513 5 11.69250607666186 39.61785757372588 34.97635590153138 13.713280448080884 6 27.99150405147336 39.158482420575126 17.79873567962651 15.051277848325004 7 24.79683386688101 35.38596134363169 22.936888650147424 16.880316139339875 8 24.878567556331916 34.10081731761434 21.25618271102189 19.76443241503185 9 25.78121451381332 16.95575668646158 22.498267039787976 34.764761759937116 10-11 23.577688951909614 27.27758369062266 28.51983474175437 20.624892615713357 12-13 25.68242097010224 25.83725183121066 28.5504454349582 19.929881763728897 14-15 22.398179545261545 26.786091631003423 28.570504604458907 22.245224219276125 16-17 19.726518142266652 30.015660825619765 29.11368218990874 21.14413884220484 18-19 19.46703768180054 28.696516400929383 32.099836380746055 19.736609536524025 20-21 20.77799545381834 27.25245329894403 31.575818737299034 20.3937325099386 22-23 20.417986348544133 27.38886900280188 31.642741646814866 20.550403001839122 24-25 20.81981361050164 27.86644455810996 31.524027419281573 19.789714412106825 26-27 20.34682988460234 27.334034089534526 32.5872369880781 19.731899037785045 28-29 19.621617511570793 28.46760918316663 32.14473688108473 19.766036424177848 30-31 20.118911771139526 27.778603485385883 31.75460781878719 20.347876924687398 32-33 20.01682654684006 28.103246269448906 31.859792600960517 20.02013458275052 34-35 20.83026797714141 27.63797559104182 31.66543394087459 19.86632249094218 36-37 20.63876331910191 27.52401861980544 31.932976213188667 19.904241847903986 38-39 20.181513714852848 28.211829925543984 31.708652792990144 19.898003566613024 40-41 19.92880750343802 28.266911060294635 31.942701953468323 19.86157948279902 42-43 19.796611543570283 28.489807096350983 30.919998596093677 20.793582763985057 44-45 20.158921999698467 27.928601263961284 30.92014107725883 20.992335659081416 46-47 20.795224481403277 28.506113233212332 30.489602363045385 20.209059922339005 48-49 20.430040560351685 28.51635907823314 30.325955758274937 20.727644603140238 50-51 19.737209551756642 28.635449921387263 30.884451383915355 20.742889142940744 52-53 20.336142094454228 29.339348153946197 29.684760543725552 20.63974920787402 54-55 19.926133079040078 28.538274923292047 29.945056845413088 21.590535152254795 56-57 19.833782279707656 28.65212828382016 30.2267832835074 21.28730615296478 58-59 19.74893659919382 28.140336305990605 31.049247206018137 21.06147988879744 60-61 20.42254639513959 28.829304993513567 30.26947245969427 20.47867615165258 62-63 20.208921089506152 28.844628949548675 30.11867531567259 20.82777464527259 64-65 19.676070094686747 29.332823223066473 30.247265077416152 20.743841604830628 66-67 20.287546528645944 28.574175066483875 29.163672124283067 21.97460628058711 68-69 20.538693500028717 28.46275554624877 29.36461023399734 21.633940719725175 70-71 20.65457887827829 28.520392747326966 29.284457433329692 21.54057094106505 72-73 19.987709855235885 28.158374537091497 30.11136169125372 21.742553916418903 74-75 19.78421416160058 29.46041953095599 29.31038912687645 21.444977180566983 76-77 18.158151692229115 29.10834372355501 30.39998597945991 22.333518604755966 78-79 18.863944215364096 29.105928811994975 30.08949582721594 21.940631145424987 80-81 19.948260753158248 29.08319674843977 29.120788467789183 21.847754030612798 82-83 19.975244787284886 28.841303978608018 29.15728595183645 22.026165282270647 84-85 19.744993050875493 28.955360206572912 28.948744343807626 22.350902398743973 86-87 19.59611579159409 29.284666184794155 28.908499520102378 22.210718503509376 88-89 19.712091050205586 28.969572910865438 28.497229233072254 22.821106805856726 90-91 19.386268777274097 28.426383879718543 29.216055147810327 22.971292195197037 92-93 18.67124414000645 29.23149892427394 29.77548276335464 22.321774172364968 94-95 18.327064923131793 28.95733484594622 29.47428977888112 23.24131045204087 96-97 18.22041059196525 29.12355302610954 28.758438467368897 23.89759791455631 98-99 18.218614966827126 29.161281405814734 28.75206000311473 23.868043624243413 100-101 18.295266578587096 28.823400127285115 28.97675028245434 23.90458301167345 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2959.0 1 2136.5 2 1276.5 3 1243.5 4 1302.5 5 1501.0 6 1959.5 7 2640.0 8 3203.0 9 3427.0 10 3415.5 11 3379.0 12 3452.5 13 3542.5 14 3552.0 15 3541.0 16 3536.5 17 3594.5 18 3680.5 19 3885.5 20 4033.5 21 4265.5 22 4806.0 23 5406.5 24 6143.0 25 7189.5 26 8478.5 27 9942.5 28 11301.0 29 12640.5 30 14457.5 31 16694.5 32 19436.0 33 22441.0 34 25336.0 35 28129.0 36 30702.0 37 32858.5 38 33768.5 39 33410.0 40 32968.5 41 32586.0 42 32159.5 43 32202.0 44 32923.5 45 34640.0 46 36911.0 47 38980.0 48 40228.5 49 38215.5 50 33775.5 51 30068.0 52 26871.0 53 24471.5 54 22931.5 55 21424.0 56 19929.5 57 18126.0 58 16136.0 59 14000.5 60 11670.0 61 9345.0 62 7161.5 63 5343.0 64 3881.5 65 2732.0 66 1891.5 67 1310.0 68 913.5 69 592.0 70 397.0 71 269.0 72 173.5 73 119.0 74 76.0 75 52.0 76 32.5 77 10.0 78 4.0 79 3.0 80 2.0 81 1.0 82 0.0 83 0.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02182246192918463 2 0.006418371155642539 3 0.0 4 0.0 5 0.14535142063239717 6 0.0 7 0.0 8 0.0014811625743790472 9 0.0 10-11 0.0 12-13 0.0 14-15 3.949766865010793E-4 16-17 0.0 18-19 9.874417162526982E-5 20-21 0.0 22-23 5.43092943938984E-4 24-25 0.0 26-27 2.4686042906317453E-4 28-29 0.0038510226933855226 30-31 0.011701184337594473 32-33 0.0032091855778212694 34-35 0.00562841778264038 36-37 0.10930979798917369 38-39 0.11000100719055059 40-41 0.12194905195720823 42-43 0.123874563303901 44-45 0.12002354061051547 46-47 0.11276584399605813 48-49 0.11246961148118231 50-51 0.10995163510473793 52-53 0.08728984771673852 54-55 0.07534180295008087 56-57 0.5475364316621212 58-59 1.560009795421825 60-61 0.5165307617717864 62-63 0.0038510226933855226 64-65 0.11572816914481622 66-67 1.9002328387566925 68-69 2.8603717915550035 70-71 2.776241757330274 72-73 2.622842686710417 74-75 1.9998163358407772 76-77 1.4004885861612018 78-79 0.9312562825979196 80-81 0.052038178446517196 82-83 1.9748834325053965E-4 84-85 4.937208581263491E-5 86-87 1.9748834325053965E-4 88-89 1.9748834325053965E-4 90-91 0.24562612691785868 92-93 0.3815968512458552 94-95 1.9921636625398185 96-97 2.422688250825995 98-99 1.4057713993431538 100-101 0.9339717473176145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1012718.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.30856028442803 #Duplication Level Percentage of deduplicated Percentage of total 1 69.32238181472569 27.249630246242823 2 14.13885624137684 11.115561658340463 3 5.849562366432522 6.898136247553029 4 3.1357709554008415 4.930505665541299 5 1.8050656986884188 3.547726691712346 6 1.2007814762451061 2.832059462844314 7 0.815471518221628 2.2438507933974243 8 0.5856036049510055 1.8415387686395983 9 0.43214031628503646 1.528813230661994 >10 2.415565217405828 17.33218066694702 >50 0.18866539488003534 5.101149725499409 >100 0.09984940175538977 6.93786716968038 >500 0.0035122905083717526 0.9715696396687219 >1k 0.00577019154946788 4.446913292892984 >5k 0.0010035115738205008 3.022496740378264 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9827 0.9703589745615264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7559 0.7464071933154145 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7320 0.722807336296975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5943 0.5868366119689785 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4860 0.4798966740988113 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4234 0.4180828226613924 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4214 0.41610793922888706 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3103 0.30640316455321226 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2591 0.25584614868107414 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2033 0.20074690091417355 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1946 0.19215615798277508 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1902 0.1878114144312632 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1873 0.18494783345413035 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1805 0.17823322978361203 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1572 0.15522583779492416 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1564 0.154435884421922 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1561 0.1541396519070462 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.15255974516104187 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1366 0.13488453844011858 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1202 0.11869049429357433 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1190 0.11750556423407109 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1143 0.1128645881676834 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1131 0.11167965810818016 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1115 0.11009975136217584 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1062 0.10486631026603654 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1046 0.10328640352003224 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1035 0.10220021763215426 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.874417162526982E-5 0.0 10-11 0.0 0.0 0.0 9.874417162526982E-5 0.0 12-13 0.0 0.0 0.0 1.4811625743790474E-4 0.0 14-15 0.0 0.0 0.0 2.4686042906317453E-4 0.0 16-17 0.0 0.0 0.0 5.430929439389841E-4 0.0 18-19 0.0 0.0 0.0 8.393254588147935E-4 0.0 20-21 0.0 0.0 0.0 0.0012343021453158727 0.0 22-23 0.0 0.0 0.0 0.0020242555183180313 0.0 24-25 0.0 0.0 0.0 0.003209185577821269 0.0 26-27 0.0 0.0 0.0 0.005282813181951935 0.0 28-29 0.0 0.0 0.0 0.012737998139659807 0.0 30-31 0.0 0.0 0.0 0.03228934412146323 0.0 32-33 0.0 0.0 0.0 0.07208324528644697 0.0 34-35 0.0 0.0 0.0 0.1349339105259312 0.0 36-37 0.0 0.0 0.0 0.22592666467861733 0.0 38-39 0.0 0.0 0.0 0.35370162276171646 0.0 40-41 0.0 0.0 0.0 0.49539950904397867 0.0 42-43 0.0 0.0 0.0 0.6264330247907117 0.0 44-45 0.0 0.0 0.0 0.7865466990810868 0.0 46-47 0.0 0.0 0.0 0.9728769509379709 0.0 48-49 0.0 0.0 0.0 1.1323981601985942 0.0 50-51 0.0 0.0 0.0 1.2902900906274009 0.0 52-53 0.0 0.0 0.0 1.4396406502106214 0.0 54-55 0.0 0.0 0.0 1.600741766217249 0.0 56-57 0.0 0.0 0.0 1.7442664196745787 0.0 58-59 0.0 0.0 0.0 1.835802266771204 0.0 60-61 0.0 0.0 0.0 1.9131683252396026 0.0 62-63 0.0 0.0 0.0 1.9784382226839061 0.0 64-65 0.0 0.0 0.0 2.043856236385647 0.0 66-67 0.0 0.0 0.0 2.0992023445816113 0.0 68-69 0.0 0.0 0.0 2.1738035662445023 0.0 70-71 0.0 0.0 0.0 2.2933827580827044 0.0 72-73 0.0 0.0 0.0 2.447719898333001 0.0 74-75 0.0 0.0 0.0 2.6611060532152093 0.0 76-77 0.0 0.0 0.0 2.9732857518084996 0.0 78-79 0.0 0.0 0.0 3.4823119565367655 0.0 80-81 0.0 0.0 0.0 4.080948497014964 0.0 82-83 0.0 0.0 0.0 4.644629600737817 0.0 84-85 0.0 0.0 0.0 5.0893733497380325 0.0 86-87 0.0 0.0 0.0 5.422783045230755 0.0 88-89 0.0 0.0 0.0 5.744047207613571 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5055 0.0 25.687174 1 TACATGG 5200 0.0 24.154236 2 ACATGGG 4965 0.0 24.064587 3 TGTCGCG 115 2.935849E-9 23.138523 90-91 GAGTACT 6330 0.0 23.038628 12-13 CATGGGA 3400 0.0 22.689705 4 AGTACTT 6595 0.0 21.970455 12-13 GTACTTT 6830 0.0 20.973835 14-15 CATGGGG 1375 0.0 20.184278 4 GCTTACG 325 0.0 19.960505 60-61 CGCCTAT 75 6.513211E-4 19.487503 66-67 AGAGTAC 10235 0.0 19.47998 10-11 GGATATG 515 0.0 19.184057 1 ATAGGCC 125 0.0063795294 18.815853 3 ACTTTTT 7795 0.0 18.800034 16-17 ATGGGAG 1180 0.0 18.736124 5 GTATCAA 15375 0.0 18.726847 1 TACTTTT 7775 0.0 18.454807 14-15 ATGGGAT 1510 0.0 18.379725 5 CGTTGCA 80 0.0010051543 18.269535 66-67 >>END_MODULE