FastQCFastQC Report
Fri 27 May 2016
SRR939700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014807
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.41288639120542137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.2868525739377044No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27770.2736480926915167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.20851255460397886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17540.17284074705830763No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17260.17008160172328335No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.1659428837207469No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12800.12613235817253923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG39050.032.210171
TATCACG753.183794E-431.4850542
CCGATAA700.00865689626.9698689
ATCACGC1109.850025E-525.761773
ACATGGG45500.023.666863
TACATGG47550.023.638622
CATGGGG14100.023.445164
GAGTACT55900.023.17533312-13
GTCCAAG3502.6287598E-622.8173641
GGATATG4053.7580867E-722.183551
AGTACTT59850.021.48808912-13
GTACTTT61150.020.95926514-15
GTATAGT2450.004025688420.3726481
AGTACAT33300.019.571792
ATGGGGA7500.019.5062775
CATGGGA31600.019.2786624
CAACGCT1250.00626105818.8770435
TAGACCC1250.00626105818.8770435
ATGGGAT12500.018.4995025
AGAGTAC97100.018.44539610-11
Produced by FastQC (version 0.11.5)