##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939700_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1014807 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.357276802387055 2.0 2.0 31.0 2.0 33.0 2 22.587754124675925 16.0 16.0 31.0 16.0 34.0 3 26.955353086843115 28.0 25.0 31.0 16.0 34.0 4 32.204836978854104 35.0 32.0 35.0 25.0 37.0 5 32.85566910752488 35.0 33.0 35.0 26.0 37.0 6 33.262648956895255 35.0 33.0 37.0 28.0 37.0 7 33.43863315881739 35.0 33.0 37.0 28.0 37.0 8 33.38197312395362 35.0 33.0 37.0 27.0 37.0 9 34.69512823620649 37.0 34.0 39.0 27.0 39.0 10-11 34.558641692459744 37.0 34.0 39.0 27.0 39.0 12-13 34.590774896113246 37.0 34.0 39.0 27.0 39.0 14-15 35.177660875417686 38.0 33.5 40.0 26.5 41.0 16-17 34.814932297471344 38.0 33.0 40.0 25.0 41.0 18-19 35.14499210194648 38.0 33.5 40.0 26.0 41.0 20-21 35.17212878902097 38.0 34.0 40.0 26.0 41.0 22-23 34.96850879034142 38.0 33.5 40.0 25.5 41.0 24-25 34.715202496632365 38.0 33.0 40.0 24.5 41.0 26-27 34.72507136825031 38.0 33.0 40.0 24.5 41.0 28-29 34.448306919443795 38.0 33.0 40.0 24.0 41.0 30-31 34.25683504351073 38.0 33.0 40.0 24.0 41.0 32-33 33.84724139663996 37.0 33.0 40.0 22.0 41.0 34-35 33.51624939520519 37.0 32.0 40.0 20.0 41.0 36-37 33.15829660221106 37.0 31.0 40.0 18.0 41.0 38-39 32.892204133396795 37.0 31.0 40.0 16.0 41.0 40-41 32.49482167545159 37.0 30.5 40.0 15.0 41.0 42-43 32.17367391040858 36.0 30.0 39.5 14.0 41.0 44-45 31.736902189283285 36.0 30.0 39.0 9.0 41.0 46-47 31.282420696743323 36.0 29.5 39.0 8.0 40.0 48-49 30.804696360982923 35.5 28.5 39.0 4.0 40.0 50-51 30.09858574093399 34.5 27.5 38.5 2.0 40.0 52-53 30.14828878791731 35.0 28.0 38.5 2.0 40.0 54-55 30.280995795259592 35.0 28.0 39.0 2.0 40.0 56-57 29.97001104643543 35.0 27.5 39.0 2.0 40.0 58-59 29.566010088617837 35.0 26.5 38.0 2.0 40.0 60-61 28.952756534001047 34.0 26.0 38.0 2.0 40.0 62-63 28.570403534859338 34.0 26.0 38.0 2.0 40.0 64-65 28.00484032924487 33.5 24.5 37.5 2.0 39.5 66-67 27.38376607571686 33.0 22.5 36.5 2.0 39.0 68-69 27.027169698277604 33.0 22.0 36.0 2.0 39.0 70-71 26.48746510420208 32.0 21.5 36.0 2.0 38.5 72-73 25.874545110548112 32.0 20.0 35.0 2.0 38.0 74-75 25.333079590503417 31.0 18.0 35.0 2.0 37.0 76-77 24.702439478639782 31.0 14.5 35.0 2.0 36.5 78-79 24.034284351605773 30.5 7.5 34.0 2.0 36.0 80-81 23.265712100921654 30.0 4.5 34.0 2.0 35.5 82-83 22.57273599807648 29.0 2.0 34.0 2.0 35.0 84-85 21.86848238137892 29.0 2.0 34.0 2.0 35.0 86-87 21.20625054813378 28.5 2.0 33.5 2.0 35.0 88-89 20.385288532696364 27.0 2.0 33.0 2.0 35.0 90-91 19.425572054587718 25.5 2.0 32.5 2.0 34.0 92-93 18.40730700517438 24.5 2.0 32.0 2.0 34.0 94-95 17.4208002112717 21.5 2.0 32.0 2.0 34.0 96-97 16.509279597007115 18.5 2.0 31.0 2.0 34.0 98-99 15.50458609371043 2.0 2.0 31.0 2.0 34.0 100-101 13.949685013997735 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.893568763560456 1101 2 8.06266408162508 1101 3 3.6575328763001664 1101 4 2.3942822116112055 1101 5 1.6842605654638447 1101 6 1.3114115806401827 1101 7 1.1141582027097172 1101 8 1.3359194087166912 1101 9 1.2462458517866608 1101 10-11 1.1866760748077425 1101 12-13 0.7632140764269977 1101 14-15 0.9740434601959933 1101 16-17 1.110473111276697 1101 18-19 1.1237802123127736 1101 20-21 0.9752084542504207 1101 22-23 0.9340174126935423 1101 24-25 1.041000641407713 1101 26-27 1.4441292349073933 1101 28-29 1.2630512054672423 1101 30-31 1.4236842292283 1101 32-33 1.6299951754705546 1101 34-35 1.5045191205937911 1101 36-37 1.3770074973560966 1101 38-39 1.3665640098143328 1101 40-41 1.643823966325165 1101 42-43 1.4363967313909782 1101 44-45 1.2348069561719655 1101 46-47 0.9844281391011371 1101 48-49 0.9828952595808218 1101 50-51 1.1448451180542207 1101 52-53 1.1119884604191945 1101 54-55 0.9576616504805671 1101 56-57 0.894584284135977 1101 58-59 1.0990609279912071 1101 60-61 1.0116625282436402 1101 62-63 0.8190596357431836 1101 64-65 0.7086126158685104 1101 66-67 0.6336275410779528 1101 68-69 0.5266122604271644 1101 70-71 0.5319930870132836 1101 72-73 0.764025221577306 1101 74-75 0.6213367000406969 1101 76-77 0.21751428107510762 1101 78-79 0.05613193413906359 1101 80-81 0.20111764517125508 1101 82-83 0.25612831622716925 1101 84-85 0.20246991289233307 1101 86-87 0.20784597194273502 1101 88-89 -0.06668732596024896 1101 90-91 0.09303040698792131 1101 92-93 -0.06352898712313504 1101 94-95 -0.48528257782904305 1101 96-97 -1.3404578836359828 1101 98-99 -1.2757436274889358 1101 100-101 -1.0573765880715076 1102 1 14.960834749761808 1102 2 7.985390116088851 1102 3 3.8327217817402186 1102 4 2.188202847175859 1102 5 1.6799975140348096 1102 6 1.2659544884025493 1102 7 1.096139975822254 1102 8 0.9463771675089276 1102 9 1.0975742274993436 1102 10-11 1.1156759337686495 1102 12-13 0.9132434434705985 1102 14-15 1.0005448019994034 1102 16-17 1.0230230787217778 1102 18-19 1.0901892411330536 1102 20-21 0.902698422120551 1102 22-23 0.712862321858573 1102 24-25 0.910294595854019 1102 26-27 0.952129822248267 1102 28-29 1.0462702584960155 1102 30-31 0.9681583949241102 1102 32-33 0.5021200549594127 1102 34-35 0.8660481968650302 1102 36-37 0.9322474859956813 1102 38-39 0.9032076078954319 1102 40-41 0.8980469278755905 1102 42-43 0.9873408238817092 1102 44-45 0.9256990187624154 1102 46-47 0.702215680132884 1102 48-49 0.4009409214695481 1102 50-51 0.2218442505672087 1102 52-53 0.6004715560147176 1102 54-55 0.5297239317975233 1102 56-57 0.7417645649390074 1102 58-59 0.3491493189283794 1102 60-61 0.4218713433800332 1102 62-63 0.044676295357898965 1102 64-65 0.005184013527262721 1102 66-67 0.15952946674455504 1102 68-69 -0.11035988145430053 1102 70-71 -0.06380433448300948 1102 72-73 0.13879216896972224 1102 74-75 0.1836571408554768 1102 76-77 0.06839249092195487 1102 78-79 0.10384130473763165 1102 80-81 0.2274562124072972 1102 82-83 0.29799470777100723 1102 84-85 -0.09277465468760937 1102 86-87 -0.1428689803261598 1102 88-89 0.05728700106110196 1102 90-91 0.05736538835337157 1102 92-93 0.12541992388119638 1102 94-95 0.06968016273437883 1102 96-97 -0.5434150676456255 1102 98-99 -0.6887833676678827 1102 100-101 -0.7854860653188105 1103 1 15.005689144721764 1103 2 8.06347340194835 1103 3 4.037227886316696 1103 4 2.3270610177018654 1103 5 1.7834737156813105 1103 6 1.501963670400471 1103 7 0.8689246233430055 1103 8 1.2783281745127155 1103 9 1.2111651482683072 1103 10-11 1.2759336329074173 1103 12-13 0.8254308143138545 1103 14-15 1.087747252766242 1103 16-17 1.286282241171648 1103 18-19 1.1079975138674314 1103 20-21 0.9572263088088846 1103 22-23 1.2758628915445769 1103 24-25 1.325613843421685 1103 26-27 1.346733722921833 1103 28-29 1.3502131000004312 1103 30-31 1.2699914434144404 1103 32-33 1.1388377141084547 1103 34-35 1.0377945637204746 1103 36-37 1.4986131127061455 1103 38-39 1.1454386546655329 1103 40-41 1.1443914426930135 1103 42-43 1.1109975936503744 1103 44-45 1.233131187032015 1103 46-47 1.2760710022288784 1103 48-49 1.244157189914528 1103 50-51 1.4221572986107276 1103 52-53 1.4496806977995114 1103 54-55 1.3478740161333924 1103 56-57 1.1767533387736684 1103 58-59 1.1798520433679123 1103 60-61 1.2438374861589807 1103 62-63 1.373139371885717 1103 64-65 1.271436820068601 1103 66-67 1.1618766289263398 1103 68-69 0.7092549102751988 1103 70-71 0.7538458783114699 1103 72-73 1.0264830417063386 1103 74-75 0.7462681458486102 1103 76-77 0.5417080076048215 1103 78-79 0.7635712065505729 1103 80-81 0.9576931347624473 1103 82-83 1.032409327477673 1103 84-85 0.8444342190096528 1103 86-87 0.7381992926316308 1103 88-89 0.879887059146732 1103 90-91 0.9159492159730895 1103 92-93 1.0193787389343107 1103 94-95 0.9575232309961663 1103 96-97 0.4310300470176429 1103 98-99 0.0920857944468807 1103 100-101 0.42448511527165067 1104 1 14.965846706390366 1104 2 8.15837632501865 1104 3 4.000990855019609 1104 4 2.416776459606382 1104 5 1.8195815980697034 1104 6 1.3895718326115016 1104 7 1.3330750387719945 1104 8 1.2612159441110649 1104 9 1.3823412643301225 1104 10-11 1.1031323930778925 1104 12-13 1.1310724691418628 1104 14-15 1.1741424914764806 1104 16-17 1.15728400620673 1104 18-19 1.1135401545321173 1104 20-21 1.3210603094115783 1104 22-23 1.117364893352665 1104 24-25 1.0580872602713143 1104 26-27 1.083883406507006 1104 28-29 1.1343998923720378 1104 30-31 1.013566285207034 1104 32-33 1.0832122028940248 1104 34-35 1.4283031951725391 1104 36-37 1.5016696368785105 1104 38-39 1.0626757290122555 1104 40-41 1.2626326498048073 1104 42-43 1.290762112140193 1104 44-45 1.2535959078551286 1104 46-47 1.166703401385103 1104 48-49 1.072781969285142 1104 50-51 1.2678458775422357 1104 52-53 1.2925064759216873 1104 54-55 1.3126701761058435 1104 56-57 0.8864588792618484 1104 58-59 0.9397480784964145 1104 60-61 0.9857710605702792 1104 62-63 0.9431596052147455 1104 64-65 0.9818849667817595 1104 66-67 0.8451765987915927 1104 68-69 1.0312715704844209 1104 70-71 1.0294819366867785 1104 72-73 0.748855937655371 1104 74-75 0.6919448861301554 1104 76-77 0.4758528265811002 1104 78-79 0.05137954957007551 1104 80-81 0.37703964128446543 1104 82-83 0.31147321313784104 1104 84-85 0.6029741905968926 1104 86-87 0.4412805703448335 1104 88-89 0.45013868427049886 1104 90-91 0.42828293675315265 1104 92-93 0.8236051369712456 1104 94-95 0.5753554665637743 1104 96-97 0.043976148873081655 1104 98-99 -0.23326721257020822 1104 100-101 -0.0625031321776568 1105 1 14.660202232346212 1105 2 7.830949208340886 1105 3 3.7031216761967727 1105 4 2.2072248236255447 1105 5 1.5888882486952411 1105 6 1.2507658945274187 1105 7 1.054609187052229 1105 8 0.9317478795741465 1105 9 1.1432728675125716 1105 10-11 1.0953606784668537 1105 12-13 0.7355188709865956 1105 14-15 1.0430348654927073 1105 16-17 1.4440443416383104 1105 18-19 1.1474270502146169 1105 20-21 1.1551688448703672 1105 22-23 1.1040754583013523 1105 24-25 1.1723140990937182 1105 26-27 1.0872751395939915 1105 28-29 1.1782892228695232 1105 30-31 1.076567123069836 1105 32-33 0.6921433191134767 1105 34-35 1.0885883191690695 1105 36-37 1.1400459017290956 1105 38-39 1.0131576791558317 1105 40-41 1.2006997289577797 1105 42-43 1.1369686345798797 1105 44-45 1.1268020221052382 1105 46-47 1.20542054033427 1105 48-49 1.1873910382548551 1105 50-51 1.1030205397116575 1105 52-53 1.294815969551781 1105 54-55 1.5815740080749627 1105 56-57 1.5170150912626212 1105 58-59 1.465389723858447 1105 60-61 1.267489896728712 1105 62-63 1.1424814376278611 1105 64-65 1.2093557669049346 1105 66-67 1.180540138153244 1105 68-69 1.5382394380877749 1105 70-71 1.293630718205101 1105 72-73 1.5740845596216957 1105 74-75 1.2251944331829527 1105 76-77 1.3248235801563375 1105 78-79 1.5547900808610287 1105 80-81 1.4823794053938606 1105 82-83 1.5282299321530282 1105 84-85 1.508282266305315 1105 86-87 1.202777909951397 1105 88-89 1.226885843056241 1105 90-91 1.5666162342803815 1105 92-93 1.6381975561031652 1105 94-95 1.58844617774459 1105 96-97 1.365950891248172 1105 98-99 0.9228715647229926 1105 100-101 0.6375903217506309 1106 1 14.717061049805636 1106 2 7.920640406681279 1106 3 3.547579153260255 1106 4 2.1961488827329347 1106 5 1.6109794306109606 1106 6 1.0961151240853937 1106 7 0.7107096132010184 1106 8 0.934200868417868 1106 9 1.044772154289106 1106 10-11 0.8974362569455394 1106 12-13 0.7048457986984076 1106 14-15 0.9968195023557413 1106 16-17 1.043993371600834 1106 18-19 0.8767038469753885 1106 20-21 0.7288384019219905 1106 22-23 0.669597396840949 1106 24-25 0.6577840678332834 1106 26-27 0.710585585505946 1106 28-29 0.9844455286040557 1106 30-31 0.6394173747064968 1106 32-33 0.7921789356545474 1106 34-35 0.6984001852794464 1106 36-37 0.86128576797514 1106 38-39 1.135224224065709 1106 40-41 1.0485738052932234 1106 42-43 0.8180350063165847 1106 44-45 0.9180292842019213 1106 46-47 0.92508578055401 1106 48-49 0.971893111869516 1106 50-51 0.8261907510721755 1106 52-53 0.6288638653193921 1106 54-55 0.5767046288382467 1106 56-57 0.6815023648208687 1106 58-59 0.7137713582202885 1106 60-61 0.9233385969481276 1106 62-63 0.6550061415821027 1106 64-65 0.5168648763485848 1106 66-67 0.49992276692176674 1106 68-69 0.7112068404725669 1106 70-71 0.9088895049575285 1106 72-73 0.7549433913634438 1106 74-75 0.7487593722242174 1106 76-77 0.804429762973637 1106 78-79 1.0880826203114786 1106 80-81 0.7689394723779515 1106 82-83 1.1355678322588965 1106 84-85 1.0257584728491196 1106 86-87 1.1726667577786074 1106 88-89 1.2022211652029462 1106 90-91 1.4253506777072111 1106 92-93 1.9839823964892886 1106 94-95 1.8942766680031546 1106 96-97 1.7361348475585494 1106 98-99 1.2732490643525445 1106 100-101 1.3964819599865486 1107 1 14.71430826840243 1107 2 7.93333831054764 1107 3 3.7978634631695023 1107 4 2.104139326866992 1107 5 1.6065551359157908 1107 6 1.0796594955124377 1107 7 0.7535227420856287 1107 8 0.8510755201859297 1107 9 0.5895150287322508 1107 10-11 0.7758788237997081 1107 12-13 0.4100221471669485 1107 14-15 0.4788272009846892 1107 16-17 0.6394121022175128 1107 18-19 0.6565296737638704 1107 20-21 0.8451599532491585 1107 22-23 0.7071479302241457 1107 24-25 0.8973498305598113 1107 26-27 0.7030281574484363 1107 28-29 1.124306922752993 1107 30-31 1.0677458359729286 1107 32-33 0.9086773061199054 1107 34-35 0.80751540266521 1107 36-37 1.1257728525455732 1107 38-39 0.861671024402554 1107 40-41 0.9158322436367641 1107 42-43 0.8178302014155179 1107 44-45 0.5303348785559194 1107 46-47 0.30158333688807204 1107 48-49 0.6199467020612701 1107 50-51 0.9584552826795445 1107 52-53 0.6423033919020504 1107 54-55 0.8849589023914461 1107 56-57 0.7024509804160601 1107 58-59 0.930454943552693 1107 60-61 0.9208147899684747 1107 62-63 0.675007057479796 1107 64-65 0.6086772597942414 1107 66-67 0.47057587830256153 1107 68-69 0.5492710398126093 1107 70-71 0.4221254987192502 1107 72-73 0.793103251129331 1107 74-75 0.3584584743900585 1107 76-77 0.39861839210376004 1107 78-79 0.4513785290473642 1107 80-81 0.5345981427489157 1107 82-83 0.534142339264239 1107 84-85 0.6309684042331156 1107 86-87 0.6501458511416551 1107 88-89 0.5253282463642073 1107 90-91 1.046770726198904 1107 92-93 1.1554893280539211 1107 94-95 1.3198701569882125 1107 96-97 0.714568001019753 1107 98-99 0.8241971456740842 1107 100-101 0.8878178222151831 1108 1 14.05111534662985 1108 2 7.34934184506254 1108 3 3.2852134474842742 1108 4 -0.4006138730022677 1108 5 -0.007329496335344743 1108 6 0.20961368806795377 1108 7 0.248678387208507 1108 8 0.2763275925252273 1108 9 0.2957265444840047 1108 10-11 0.2716310825551105 1108 12-13 -0.019039958135969925 1108 14-15 0.052174133659733 1108 16-17 0.292696228270934 1108 18-19 0.2554135587081774 1108 20-21 0.245639215074263 1108 22-23 -0.6964511322753353 1108 24-25 0.002017541813593482 1108 26-27 0.413004949876175 1108 28-29 0.27353711460554564 1108 30-31 0.1651297693690026 1108 32-33 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2312 5 -1.9186959785043207 2312 6 -0.6768188974815317 2312 7 -0.2643561153782841 2312 8 -0.1842955310107186 2312 9 -0.3021449502300868 2312 10-11 -0.18587618763843494 2312 12-13 -0.9526423920600777 2312 14-15 -0.2216792495921922 2312 16-17 -0.6795953398756467 2312 18-19 -0.38069381234898714 2312 20-21 -0.32486555866600497 2312 22-23 -0.12760935148247654 2312 24-25 -0.29411421247507263 2312 26-27 -0.03733385449709914 2312 28-29 -0.9717087836855924 2312 30-31 -0.38397761897009275 2312 32-33 -0.34158369189864146 2312 34-35 -1.0380357154637139 2312 36-37 -0.4297358825037563 2312 38-39 -0.48929259685322535 2312 40-41 -0.27322974960687674 2312 42-43 -0.585035923764103 2312 44-45 -0.5095155535999822 2312 46-47 -0.543147501753996 2312 48-49 -0.18520042348844257 2312 50-51 -0.22463867423979877 2312 52-53 -0.1690291696820374 2312 54-55 -0.3679511367405013 2312 56-57 -0.49384793747787015 2312 58-59 -0.5688672112532593 2312 60-61 -0.4348223332217991 2312 62-63 -0.41343635270492385 2312 64-65 -0.5114631070312754 2312 66-67 -0.5701575675578106 2312 68-69 -0.20186310803970997 2312 70-71 -0.3051033612303584 2312 72-73 -0.7382184838702877 2312 74-75 -0.42623926899305786 2312 76-77 -0.14731844282475848 2312 78-79 0.0391137133163042 2312 80-81 -0.3001536151901192 2312 82-83 -0.2255256349571262 2312 84-85 -0.03427944327302157 2312 86-87 -0.015680808296764326 2312 88-89 0.5668105572876136 2312 90-91 0.8394739870367367 2312 92-93 1.3373368862724568 2312 94-95 1.5283998799870488 2312 96-97 1.4626159410095418 2312 98-99 1.4596813551953218 2312 100-101 1.3774220916140614 2313 1 -14.18396602769203 2313 2 -7.461970906016404 2313 3 -3.832813490330633 2313 4 -1.2907958517203575 2313 5 -0.6658723468796452 2313 6 -1.0738649443972506 2313 7 -0.6892040736754126 2313 8 -0.21296625212278997 2313 9 -2.6887652803777797 2313 10-11 -0.6897858314264411 2313 12-13 -1.0725381348924046 2313 14-15 -1.243833897724251 2313 16-17 -1.1192130635941524 2313 18-19 -0.406758104269052 2313 20-21 -1.1902000504123151 2313 22-23 -2.1006762498317357 2313 24-25 -1.3240881481831508 2313 26-27 -0.5716518388585214 2313 28-29 -0.10333345788193782 2313 30-31 -1.5186431272237826 2313 32-33 -0.7008365155302698 2313 34-35 -1.5054553505636292 2313 36-37 -1.668224153572389 2313 38-39 -1.222133604672507 2313 40-41 -0.8010316609882793 2313 42-43 -0.39824183167026916 2313 44-45 -0.655041183487036 2313 46-47 -1.3537469804681557 2313 48-49 -0.9037060707516922 2313 50-51 -1.1933615239357174 2313 52-53 -0.4361881618627521 2313 54-55 -1.3401492253590952 2313 56-57 -1.0325099704926366 2313 58-59 -0.7443667768483913 2313 60-61 -2.385300178573665 2313 62-63 -1.2140445195163956 2313 64-65 -0.9402207091164172 2313 66-67 -1.9793669507029001 2313 68-69 -1.275711935146571 2313 70-71 -1.0249122230896113 2313 72-73 -2.0201472414723725 2313 74-75 -1.1521297989669925 2313 76-77 -0.6668666617648107 2313 78-79 -0.668531812313585 2313 80-81 -0.5173560478573656 2313 82-83 -0.3619287626721537 2313 84-85 -0.4030891739420035 2313 86-87 -0.3175921897042393 2313 88-89 -0.0370122055273292 2313 90-91 0.515407957497203 2313 92-93 0.9641831069501308 2313 94-95 1.1057239460165889 2313 96-97 0.6706958715047655 2313 98-99 0.9010366983751652 2313 100-101 0.5689946372265737 2314 1 -14.18396602769203 2314 2 -7.238037879447987 2314 3 -3.558344697761637 2314 4 -1.4910074612861521 2314 5 -1.6558013557776654 2314 6 -6.563185633254577 2314 7 -2.4624930011780677 2314 8 -5.186869782064548 2314 9 -2.07603913318885 2314 10-11 -0.5958203049384778 2314 12-13 -3.172942353599545 2314 14-15 -3.5097942542580185 2314 16-17 -2.9797124070069074 2314 18-19 -1.7381680277004108 2314 20-21 -2.4147708936480825 2314 22-23 -1.44065781635544 2314 24-25 -3.413203586487704 2314 26-27 -5.023385780818359 2314 28-29 -2.5426060327612348 2314 30-31 -2.249393705281051 2314 32-33 -4.7041121733816595 2314 34-35 -2.9773182502429165 2314 36-37 -1.205318651570451 2314 38-39 -1.70682370621752 2314 40-41 -3.2930591441828625 2314 42-43 -2.2744019684733274 2314 44-45 -3.0908668668510906 2314 46-47 -2.7645085757070156 2314 48-49 -1.6861627030490425 2314 50-51 -2.78751399008355 2314 52-53 -1.5798455443704107 2314 54-55 -0.7987233201191124 2314 56-57 -1.5581490513011396 2314 58-59 -1.894434887240859 2314 60-61 -0.9706078705277541 2314 62-63 -3.2684558358014435 2314 64-65 -1.6242026617777796 2314 66-67 -2.7085013434631264 2314 68-69 -2.297092775670187 2314 70-71 -3.1397410583789167 2314 72-73 -2.970339314311417 2314 74-75 -2.2179643543034295 2314 76-77 -1.0678598936297483 2314 78-79 -0.94747604760013 2314 80-81 -0.6547559317096088 2314 82-83 -0.8033262438900834 2314 84-85 -0.7306737296615111 2314 86-87 -0.5508522794659179 2314 88-89 -0.007032906796222704 2314 90-91 0.2893147818673931 2314 92-93 0.566382023006561 2314 94-95 0.3490956326260388 2314 96-97 0.332021350307798 2314 98-99 0.5172059063486021 2314 100-101 0.60293491153754 2315 1 -14.18396602769203 2315 2 -7.366258432456366 2315 3 -4.715684361100713 2315 4 -3.016276385881973 2315 5 -3.4219511545584282 2315 6 -3.0109963772315496 2315 7 -3.455713174819664 2315 8 -3.8993205998709435 2315 9 -2.7315284156901285 2315 10-11 -4.701473953904973 2315 12-13 -4.59008709983414 2315 14-15 -3.3578497285206907 2315 16-17 -4.502427186672229 2315 18-19 -1.6017860564486668 2315 20-21 -3.2920933538648995 2315 22-23 -4.3420112267079745 2315 24-25 -4.619687119646684 2315 26-27 -4.512528535764449 2315 28-29 -2.475544437531653 2315 30-31 -3.3189351205204467 2315 32-33 -4.308933150971146 2315 34-35 -3.705455884511661 2315 36-37 -5.368517596156575 2315 38-39 -3.6226739600835742 2315 40-41 -4.416251925994317 2315 42-43 -3.001494795653951 2315 44-45 -1.507838575379722 2315 46-47 -1.1444893131650744 2315 48-49 -0.5626261684794365 2315 50-51 -0.4023026363325428 2315 52-53 -0.19141150851173094 2315 54-55 -1.6750547813935093 2315 56-57 -3.351205245586481 2315 58-59 -2.641754931897747 2315 60-61 -4.092931592719779 2315 62-63 -1.9001174003719932 2315 64-65 -2.551212105688016 2315 66-67 -1.4142458856138695 2315 68-69 -0.9047957794808887 2315 70-71 -1.4103685465217666 2315 72-73 -1.4949555731975082 2315 74-75 -2.0678647898784384 2315 76-77 -1.755397152867328 2315 78-79 -1.2105642016013647 2315 80-81 -0.9011414953325136 2315 82-83 -0.2390158320525977 2315 84-85 -0.0815620673072388 2315 86-87 -1.1142251133977261 2315 88-89 -1.9542792459067684 2315 90-91 -1.8967015262589548 2315 92-93 -0.7999689950125877 2315 94-95 -0.6202584992975986 2315 96-97 -1.6749469981150433 2315 98-99 -0.7230230763445284 2315 100-101 -0.010210070059299525 2316 1 -14.18396602769203 2316 2 -7.35471162880782 2316 3 -5.255041983455687 2316 4 -2.8371184400832163 2316 5 -2.3598358446018004 2316 6 -1.2288420432475604 2316 7 -4.893158883300263 2316 8 -2.8273849662690544 2316 9 -1.5387748273161321 2316 10-11 -4.650923657776456 2316 12-13 -3.6407044249214877 2316 14-15 -1.7977139038281607 2316 16-17 -3.2701145016161206 2316 18-19 -2.3003638026793496 2316 20-21 -3.2829491315674204 2316 22-23 -3.121921446648198 2316 24-25 -3.6091992185674755 2316 26-27 -4.184174201315514 2316 28-29 -4.0505289201339565 2316 30-31 -2.730292615791612 2316 32-33 -3.203425307808061 2316 34-35 -4.345659156609713 2316 36-37 -2.9673868602406017 2316 38-39 -3.484554784919471 2316 40-41 -2.8642300923764665 2316 42-43 -7.1296254935997645 2316 44-45 -3.749925864906853 2316 46-47 -4.612999650735311 2316 48-49 -4.29846775386542 2316 50-51 -3.416861868622366 2316 52-53 -4.476390357581888 2316 54-55 -4.821558000720785 2316 56-57 -4.941151521682247 2316 58-59 -3.454152887088309 2316 60-61 -3.3124742924912596 2316 62-63 -2.635629265272609 2316 64-65 -2.4710051760560248 2316 66-67 -2.905439907715607 2316 68-69 -2.8465766730505244 2316 70-71 -2.809600774355136 2316 72-73 -3.8590351803535654 2316 74-75 -4.392450392058077 2316 76-77 -4.236155579724787 2316 78-79 -4.764129638525564 2316 80-81 -4.545553256163892 2316 82-83 -4.161357940109479 2316 84-85 -3.737088236885082 2316 86-87 -4.091685173049623 2316 88-89 -3.821346484863845 2316 90-91 -3.0530731131095017 2316 92-93 -2.721654374253138 2316 94-95 -2.0516820626500376 2316 96-97 -2.1311848370084228 2316 98-99 -2.187627136672141 2316 100-101 -1.9169464416771458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7318.0 3 1997.0 4 2148.0 5 2694.0 6 3396.0 7 4562.0 8 5734.0 9 6932.0 10 8542.0 11 10250.0 12 11966.0 13 13256.0 14 14577.0 15 16196.0 16 17919.0 17 18825.0 18 16377.0 19 15956.0 20 16968.0 21 18339.0 22 20062.0 23 22526.0 24 24825.0 25 27596.0 26 30687.0 27 34448.0 28 38463.0 29 42807.0 30 48361.0 31 54488.0 32 61896.0 33 71066.0 34 81663.0 35 90769.0 36 91744.0 37 53007.0 38 6417.0 39 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.8307976947983 19.92924380883327 14.234787210547589 21.005171285820843 2 15.247567328726728 22.591048874353245 39.447542474351664 22.713841322568364 3 19.161854472947628 27.86494483366237 31.36830078997919 21.60489990341081 4 11.962956380941097 17.71123353671781 39.67883024307407 30.646979839267026 5 11.959737913629102 38.980463890113946 35.067322178580625 13.992476017676328 6 27.930076859004977 38.80234958807205 18.294476178445528 14.973097374477442 7 24.97658420556551 35.22288184523369 23.38727108531837 16.413262863882434 8 24.938809912004707 33.44620601715691 21.510092416771613 20.104891654066773 9 26.39497281844458 16.921936783270887 22.72741177712802 33.955678621156515 10-11 23.62338159522762 26.98007297498367 28.714264853014015 20.682280576774694 12-13 25.95807417100125 25.73727427836674 28.620057412906156 19.684594137725846 14-15 22.86487716083101 26.50354560585179 28.722442049291597 21.909135184025605 16-17 19.98447706049012 29.675163000733217 29.238734520234587 21.101625418542067 18-19 19.63488187224073 28.413526848474053 32.347697620174316 19.603893659110906 20-21 20.98461413923634 27.036142532568658 31.911679996209187 20.06756333198582 22-23 20.553658431827706 27.048486335525464 31.99269740385598 20.405157828790852 24-25 21.006745976290073 27.4766809263335 31.915119510098222 19.601453587278197 26-27 20.431860714303347 26.982505345177486 33.02524701386042 19.560386926658733 28-29 19.801285982475008 27.985524549901196 32.65819747168459 19.55499199593921 30-31 20.16085224231147 27.397919172884063 32.198399576486295 20.24282900831817 32-33 20.1671572454311 27.721823323502004 32.26112122554328 19.849898205523616 34-35 20.909803673736203 27.305120361172385 32.045261093589225 19.73981487150218 36-37 20.783380404481115 27.128136698365026 32.32580836735933 19.76267452979453 38-39 20.301560276265704 27.857252517733105 32.135757413003006 19.70542979299818 40-41 20.135479227407046 28.008864105113396 32.092839110272116 19.76281755720744 42-43 19.891235616510034 28.261611069345317 31.12851069207102 20.71864262207363 44-45 20.31999731426594 27.662419026845985 31.11630265260672 20.90128100628136 46-47 20.96969810698353 28.307099873482855 30.602976165197216 20.120225854336397 48-49 20.68459075719019 28.275159794305726 30.484501569670712 20.555747878833373 50-51 19.95079876685767 28.534542110467186 30.98393576280679 20.53072335986835 52-53 20.43025679356082 29.295787589669764 29.772313760323044 20.50164185644637 54-55 19.95276849151591 28.424358457611177 30.208802585927558 21.41407046494535 56-57 20.64339747686102 28.52995992963735 29.62846269861102 21.198179894890607 58-59 21.098271745349123 28.14027636475646 29.772865153092532 20.98858673680189 60-61 20.581277208336996 28.582466007166364 30.409750520099486 20.426506264397155 62-63 20.331364931441655 28.536396674205545 30.357230137136444 20.775008257216363 64-65 20.233424427076958 29.05938140502885 30.058565905402045 20.648628262492153 66-67 20.323411490616536 28.513412038620984 29.413422949340195 21.74975352142228 68-69 20.199743508484065 28.555463081709036 29.80193484246702 21.442858567339883 70-71 20.87812748553545 28.532502842551526 29.429332169487658 21.160037502425364 72-73 20.726136980889834 27.87353710927856 29.93704014616562 21.463285763665993 74-75 20.00752782925856 29.549150204891312 29.130278865569963 21.31304310028017 76-77 20.36753838980171 28.549080857655433 29.284819530391758 21.798561222151093 78-79 20.331693814711016 28.615148440810874 29.501259027807276 21.55189871667083 80-81 20.74851370121492 28.590876130900988 29.06818105480288 21.592429113081206 82-83 20.54192026528519 28.309187238138794 29.45914162619543 21.689750870380585 84-85 20.367977412969275 28.4952398069753 29.074432423331043 22.062350356724387 86-87 20.268087432611477 28.651844360351625 29.131298546032593 21.94876966100431 88-89 20.51735615223399 28.267476152945104 28.71814549024488 22.497022204576027 90-91 20.533110936314163 27.820563527580145 29.14714397096198 22.49918156514371 92-93 20.90582441961458 28.364018449089468 29.017715846298405 21.71244128499755 94-95 20.788027303895877 27.865474125858924 28.966748383406447 22.37975018683875 96-97 20.445481154430702 27.584731442484994 29.198675444334864 22.77111195874944 98-99 20.64522473700831 28.04091635314891 28.330210922130146 22.98364798771264 100-101 20.698473346570193 27.518454351115462 28.760027712411585 23.02304458990276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5354.0 1 3932.0 2 2250.5 3 1772.0 4 1574.0 5 1701.5 6 2332.5 7 4399.0 8 5695.5 9 4830.0 10 3751.0 11 3191.5 12 2993.0 13 2810.0 14 2744.5 15 2738.0 16 2756.0 17 2910.5 18 3033.0 19 3174.0 20 3556.0 21 4048.5 22 4573.0 23 5213.5 24 5953.0 25 7120.5 26 8706.5 27 10088.0 28 11045.5 29 12643.5 30 14746.5 31 16682.5 32 19126.0 33 22463.5 34 25614.0 35 28034.5 36 30931.5 37 32913.0 38 33316.0 39 32631.5 40 31380.5 41 30038.5 42 28888.5 43 28926.5 44 29585.0 45 30664.0 46 33034.5 47 40073.0 48 44539.0 49 39792.0 50 32661.0 51 27771.5 52 25507.0 53 24105.0 54 23003.5 55 21852.0 56 20497.5 57 19261.0 58 17854.5 59 16035.0 60 13669.0 61 11245.5 62 8918.0 63 6718.0 64 5045.5 65 3709.5 66 2631.5 67 1941.5 68 1434.0 69 997.5 70 674.0 71 472.0 72 350.5 73 258.0 74 195.0 75 139.5 76 85.0 77 49.0 78 29.0 79 24.0 80 19.5 81 13.5 82 9.5 83 3.5 84 2.5 85 2.5 86 3.5 87 3.5 88 2.0 89 2.0 90 1.5 91 1.5 92 1.5 93 1.5 94 3.0 95 4.0 96 2.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.704504403300334 2 0.08878535524488894 3 0.12179655836035819 4 0.19185914168901083 5 0.12297904921822574 6 0.1500777980443572 7 0.1578625295253186 8 0.15480776147582742 9 0.1415047393248174 10-11 0.1289900444123858 12-13 0.1424901483730404 14-15 0.1488460367340785 16-17 0.14465804827913092 18-19 0.14963436397265686 20-21 0.1804776671820356 22-23 0.1987077345741604 24-25 0.22442691073277973 26-27 0.21797248146691933 28-29 0.21639582698976262 30-31 0.22910760371183883 32-33 0.21949986549166492 34-35 0.2074286046509336 36-37 0.19496318019091313 38-39 0.1913171667124882 40-41 0.19294309164205609 42-43 0.185601794232795 44-45 0.2018610435284739 46-47 0.2262499174719922 48-49 0.22994520140282834 50-51 0.2398485623374691 52-53 0.2651243044243881 54-55 0.25216617544025616 56-57 0.23038863547452867 58-59 0.1878682350437078 60-61 0.19905262774103843 62-63 0.20264937076705228 64-65 0.1902824872118541 66-67 0.20117125719471782 68-69 0.18801604640094124 70-71 0.204226025244209 72-73 0.22240682218392266 74-75 0.25256033905954534 76-77 0.25216617544025616 78-79 0.24812599834254195 80-81 0.2523632572499007 82-83 0.24265697812490455 84-85 0.21585385201323995 86-87 0.22679189244851483 88-89 0.22792511285397127 90-91 0.21713488377592982 92-93 0.21506552477466157 94-95 0.25329939584571254 96-97 0.28034887421943283 98-99 0.29912091658808027 100-101 0.29424314179937666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1014807.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.74025213273514 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75991103154973 58.26417655056928 2 8.976257670723877 12.340604309879142 3 2.8410898386535046 5.858916955223811 4 1.2565360107344277 3.45498408766983 5 0.6596043779005605 2.267068562237022 6 0.3851049323596757 1.5883326088778411 7 0.22214123567281044 1.0689031184458428 8 0.16939234074738854 0.931525776986374 9 0.115410187943158 0.7139992876109127 >10 0.5602220764899043 6.855463825672964 >50 0.03745203232643319 1.7632966582321248 >100 0.013361959784912426 1.660680260845219 >500 0.002109783068205681 1.0037078346225767 >1k 0.0014065220454704541 2.2283401631271604 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4190 0.41288639120542137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2911 0.2868525739377044 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2777 0.2736480926915167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2116 0.20851255460397886 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1754 0.17284074705830763 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1726 0.17008160172328335 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1684 0.1659428837207469 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1280 0.12613235817253923 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.478113572334444E-4 0.0 18-19 0.0 0.0 0.0 2.9562271446688875E-4 9.854090482229626E-5 20-21 0.0 0.0 0.0 8.375976909895182E-4 9.854090482229626E-5 22-23 0.0 0.0 0.0 0.001527384024745592 9.854090482229626E-5 24-25 0.0 0.0 0.0 0.002463522620557407 9.854090482229626E-5 26-27 0.0 0.0 0.0 0.005074856598348257 9.854090482229626E-5 28-29 0.0 9.854090482229626E-5 0.0 0.013007399436543107 9.854090482229626E-5 30-31 0.0 9.854090482229626E-5 0.0 0.03133600773349021 9.854090482229626E-5 32-33 0.0 9.854090482229626E-5 0.0 0.0672541675412172 9.854090482229626E-5 34-35 0.0 9.854090482229626E-5 0.0 0.12273269695616998 9.854090482229626E-5 36-37 0.0 9.854090482229626E-5 0.0 0.20363477981527522 9.854090482229626E-5 38-39 0.0 9.854090482229626E-5 0.0 0.3227707337454314 9.854090482229626E-5 40-41 0.0 1.9708180964459252E-4 0.0 0.4425472035569325 9.854090482229626E-5 42-43 0.0 1.9708180964459252E-4 0.0 0.568778102634294 9.854090482229626E-5 44-45 0.0 1.9708180964459252E-4 0.0 0.719102252940707 9.854090482229626E-5 46-47 0.0 1.9708180964459252E-4 0.0 0.8972642088594186 9.854090482229626E-5 48-49 0.0 1.9708180964459252E-4 0.0 1.07498273070643 9.854090482229626E-5 50-51 0.0 1.9708180964459252E-4 0.0 1.2715225653744997 9.854090482229626E-5 52-53 0.0 1.9708180964459252E-4 0.0 1.4622484866580543 9.854090482229626E-5 54-55 0.0 1.9708180964459252E-4 0.0 1.6619909007328486 9.854090482229626E-5 56-57 0.0 1.9708180964459252E-4 0.0 1.9087373264078784 9.854090482229626E-5 58-59 0.0 1.9708180964459252E-4 0.0 2.165288572112727 9.854090482229626E-5 60-61 0.0 1.9708180964459252E-4 0.0 2.460615663865149 9.854090482229626E-5 62-63 0.0 1.9708180964459252E-4 0.0 2.7500795717806437 9.854090482229626E-5 64-65 0.0 1.9708180964459252E-4 0.0 3.038114636576216 9.854090482229626E-5 66-67 0.0 1.9708180964459252E-4 0.0 3.3721683039238 9.854090482229626E-5 68-69 0.0 1.9708180964459252E-4 0.0 3.7431255401273344 9.854090482229626E-5 70-71 0.0 1.9708180964459252E-4 0.0 4.147340331708394 9.854090482229626E-5 72-73 0.0 1.9708180964459252E-4 0.0 4.5598325592945255 9.854090482229626E-5 74-75 0.0 1.9708180964459252E-4 0.0 4.994545760918086 9.854090482229626E-5 76-77 0.0 1.9708180964459252E-4 0.0 5.424085565038475 9.854090482229626E-5 78-79 0.0 1.9708180964459252E-4 0.0 5.950638889956416 9.854090482229626E-5 80-81 0.0 1.9708180964459252E-4 0.0 6.515327545040584 9.854090482229626E-5 82-83 0.0 1.9708180964459252E-4 0.0 7.054986810299889 9.854090482229626E-5 84-85 0.0 1.9708180964459252E-4 0.0 7.630810587628978 9.854090482229626E-5 86-87 0.0 1.9708180964459252E-4 0.0 8.222745802896512 9.854090482229626E-5 88-89 0.0 1.9708180964459252E-4 0.0 8.830201210673557 9.854090482229626E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3905 0.0 32.21017 1 TATCACG 75 3.183794E-4 31.485054 2 CCGATAA 70 0.008656896 26.969868 9 ATCACGC 110 9.850025E-5 25.76177 3 ACATGGG 4550 0.0 23.66686 3 TACATGG 4755 0.0 23.63862 2 CATGGGG 1410 0.0 23.44516 4 GAGTACT 5590 0.0 23.175333 12-13 GTCCAAG 350 2.6287598E-6 22.817364 1 GGATATG 405 3.7580867E-7 22.18355 1 AGTACTT 5985 0.0 21.488089 12-13 GTACTTT 6115 0.0 20.959265 14-15 GTATAGT 245 0.0040256884 20.372648 1 AGTACAT 3330 0.0 19.57179 2 ATGGGGA 750 0.0 19.506277 5 CATGGGA 3160 0.0 19.278662 4 CAACGCT 125 0.006261058 18.877043 5 TAGACCC 125 0.006261058 18.877043 5 ATGGGAT 1250 0.0 18.499502 5 AGAGTAC 9710 0.0 18.445396 10-11 >>END_MODULE