##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939700_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1014807 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45767126162906 31.0 31.0 34.0 30.0 34.0 2 31.65545172628884 31.0 31.0 34.0 30.0 34.0 3 31.68919114669095 31.0 31.0 34.0 30.0 34.0 4 35.499540306679 37.0 35.0 37.0 33.0 37.0 5 35.263240202324184 37.0 35.0 37.0 33.0 37.0 6 35.28853466718302 37.0 35.0 37.0 32.0 37.0 7 35.201179140467104 37.0 35.0 37.0 32.0 37.0 8 35.17352659175587 37.0 35.0 37.0 32.0 37.0 9 36.739394781470764 39.0 35.0 39.0 32.0 39.0 10-11 36.624615813647324 39.0 35.0 39.0 32.0 39.0 12-13 36.56429744769203 39.0 35.0 39.0 32.0 39.0 14-15 37.54380241760256 39.0 36.0 41.0 32.0 41.0 16-17 37.52365474420259 39.0 36.0 41.0 32.0 41.0 18-19 37.56002668487702 39.0 36.0 41.0 32.0 41.0 20-21 37.524015896618764 39.0 36.0 41.0 32.0 41.0 22-23 37.369850621842374 39.0 36.0 41.0 32.0 41.0 24-25 37.21146681093055 39.0 36.0 41.0 31.0 41.0 26-27 36.90017954152859 39.0 35.5 40.0 31.0 41.0 28-29 36.701276695962875 39.0 35.0 40.0 30.0 41.0 30-31 36.41020164425353 38.0 35.0 40.0 30.0 41.0 32-33 36.13810704892654 38.0 35.0 40.0 30.0 41.0 34-35 36.01642479801578 38.0 35.0 40.0 30.0 41.0 36-37 35.841887176576435 38.0 35.0 40.0 30.0 41.0 38-39 35.792769955272284 38.0 35.0 40.0 29.0 41.0 40-41 35.67771852184701 38.0 35.0 40.0 29.0 41.0 42-43 35.42650868588805 38.0 34.0 40.0 27.0 41.0 44-45 35.126831505892255 38.0 34.0 40.0 26.5 41.0 46-47 34.71169394771617 38.0 33.5 40.0 24.5 41.0 48-49 34.30466679871148 38.0 33.0 40.0 24.0 41.0 50-51 34.03905126787655 38.0 33.0 40.0 23.0 41.0 52-53 33.82268697397633 38.0 33.0 40.0 23.0 41.0 54-55 33.54047814017838 37.0 33.0 40.0 21.5 41.0 56-57 32.729035176146795 36.5 32.0 40.0 14.5 41.0 58-59 29.778948115257386 34.5 25.5 39.0 6.5 40.5 60-61 29.47434339731594 34.0 25.0 38.0 7.0 40.0 62-63 31.20417330586013 35.0 29.5 38.5 7.5 40.0 64-65 31.236127657771377 35.0 30.5 39.0 2.0 40.0 66-67 26.503231648973646 31.5 18.0 36.5 2.0 39.5 68-69 19.295529100607308 19.0 7.5 32.0 2.0 36.5 70-71 17.394096611473906 15.0 7.0 29.0 2.0 35.0 72-73 19.08939187451407 19.5 7.5 31.0 2.0 35.0 74-75 20.45291025781257 22.5 7.5 32.5 2.0 36.0 76-77 22.14360464600658 27.0 11.0 33.0 2.0 35.5 78-79 24.21715459195689 30.5 11.5 34.5 2.0 36.0 80-81 25.73352814870217 31.5 20.0 35.0 2.0 36.0 82-83 26.816903608272312 33.0 25.5 35.0 2.0 36.0 84-85 26.842538039252783 33.0 26.0 35.0 2.0 36.0 86-87 26.58961457695897 33.0 25.0 35.0 2.0 35.5 88-89 26.146457405201186 33.0 24.5 35.0 2.0 35.0 90-91 24.38214704865063 32.0 11.0 34.5 2.0 35.0 92-93 22.857868540520514 31.0 2.0 34.0 2.0 35.0 94-95 19.283381470565338 26.5 2.0 34.0 2.0 35.0 96-97 16.687053794465353 13.5 2.0 33.0 2.0 35.0 98-99 16.975227801936725 18.5 2.0 33.0 2.0 35.0 100-101 16.245040682612554 2.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1897905497355481 1101 2 0.14486123412331509 1101 3 0.21537037068686615 1101 4 0.20686091424435915 1101 5 0.2843113123385592 1101 6 0.27607315946108457 1101 7 0.24578775650611107 1101 8 0.29659374835853214 1101 9 0.41958879436082697 1101 10-11 0.36626740650011413 1101 12-13 0.27629471835000885 1101 14-15 0.5577474576428116 1101 16-17 0.5309101968208623 1101 18-19 0.47990112349972947 1101 20-21 0.3352633875211595 1101 22-23 0.20779167081298766 1101 24-25 0.4261658903525003 1101 26-27 0.5315135022520465 1101 28-29 0.3492906393597508 1101 30-31 0.12456103395812335 1101 32-33 0.28171413398828804 1101 34-35 0.1509441937534035 1101 36-37 0.2619950691752493 1101 38-39 0.19545845170444665 1101 40-41 0.22386041902053222 1101 42-43 0.4576922114944253 1101 44-45 0.25774775798762306 1101 46-47 0.21886127246603593 1101 48-49 -0.14103809295657754 1101 50-51 -0.15237748033833753 1101 52-53 -0.3148215669940768 1101 54-55 -0.14063529801509844 1101 56-57 -3.4365464978914417 1101 58-59 -9.711952150928315 1101 60-61 -11.044712215119297 1101 62-63 -3.638877193508403 1101 64-65 -0.9117342157417845 1101 66-67 -2.227385605627564 1101 68-69 -4.8216313212295425 1101 70-71 -4.725495048210899 1101 72-73 -3.489620163786773 1101 74-75 -5.826500695414145 1101 76-77 -5.029374051950249 1101 78-79 0.7342667370698024 1101 80-81 1.3895296414545335 1101 82-83 0.14126541746302834 1101 84-85 -0.22285593741478493 1101 86-87 -0.40548264025980174 1101 88-89 -0.524168373977691 1101 90-91 0.9251733236060389 1101 92-93 0.4860685524879109 1101 94-95 -8.412141102823472 1101 96-97 -9.263938969300249 1101 98-99 -8.602679006372123 1101 100-101 -9.309536426700218 1102 1 0.2864409679261115 1102 2 0.38368485218172665 1102 3 0.2403381538808027 1102 4 0.21977371924139533 1102 5 0.3133460446637528 1102 6 0.31815787178963717 1102 7 0.3341815917454056 1102 8 0.3748028223885953 1102 9 0.3427476063093522 1102 10-11 0.2781484042840532 1102 12-13 0.3379225219849502 1102 14-15 0.44290830982804863 1102 16-17 0.40315727798789425 1102 18-19 0.45454451678433827 1102 20-21 0.39027153107962675 1102 22-23 0.4514700282678703 1102 24-25 0.2449765444067893 1102 26-27 -0.12268328012462604 1102 28-29 0.29893050666707666 1102 30-31 0.3902110599440931 1102 32-33 0.11763543803959209 1102 34-35 0.2530893403664436 1102 36-37 -0.19391572456570572 1102 38-39 -0.05556214966305362 1102 40-41 -0.02088732387100123 1102 42-43 0.030909371121701668 1102 44-45 -0.030498469216688306 1102 46-47 -0.30119429307173107 1102 48-49 -0.06130029042289209 1102 50-51 -0.5899531922476413 1102 52-53 -0.6735612571331302 1102 54-55 -0.8326242776455715 1102 56-57 -4.233306269678604 1102 58-59 -10.094972783208494 1102 60-61 -8.030617000208565 1102 62-63 -2.880931186363707 1102 64-65 -1.376294763050737 1102 66-67 -2.4196426561330817 1102 68-69 -5.195808512696871 1102 70-71 -5.212857076237105 1102 72-73 -3.761926558653723 1102 74-75 -4.3632882306497685 1102 76-77 -4.631311987022034 1102 78-79 1.1100977567824302 1102 80-81 1.3628075638134831 1102 82-83 -0.12018772954414203 1102 84-85 -0.26476869797484426 1102 86-87 -0.1768973975856234 1102 88-89 -0.31954238839459137 1102 90-91 0.836838415658196 1102 92-93 0.33941253137455263 1102 94-95 -8.407721072954098 1102 96-97 -8.967902264359823 1102 98-99 -7.964884297463282 1102 100-101 -9.083281710101849 1103 1 0.27342475980082526 1103 2 0.2235919151711272 1103 3 0.24103629849168584 1103 4 0.17753876286147374 1103 5 0.13902212790492996 1103 6 0.22853844437992166 1103 7 0.1660516139743322 1103 8 0.334871743224582 1103 9 0.35088797247223624 1103 10-11 0.4830161477689714 1103 12-13 0.47391550702790397 1103 14-15 0.5873809593147783 1103 16-17 0.44033487480658806 1103 18-19 0.3544640905251626 1103 20-21 0.377864105055032 1103 22-23 0.2872440751608494 1103 24-25 0.30162958329065503 1103 26-27 0.31268947324601015 1103 28-29 0.2967676546625668 1103 30-31 0.24072992101889668 1103 32-33 0.09358714877314611 1103 34-35 0.18055770045268105 1103 36-37 0.32504588306584736 1103 38-39 0.4374871225910013 1103 40-41 0.429710567173764 1103 42-43 0.5075396784170323 1103 44-45 0.5113916037254569 1103 46-47 0.5562878623520149 1103 48-49 0.3017991365400121 1103 50-51 0.17045754018982961 1103 52-53 0.24898239086794405 1103 54-55 0.2786278586769342 1103 56-57 0.7812654046397327 1103 58-59 -8.193497743274623 1103 60-61 -7.385285632850458 1103 62-63 -1.8454528735279006 1103 64-65 -0.18006865355932433 1103 66-67 -1.555602130234707 1103 68-69 -3.0380054900099616 1103 70-71 -3.9575386105914045 1103 72-73 -2.9520857914765735 1103 74-75 -3.746601736676155 1103 76-77 -0.4728596898470059 1103 78-79 3.373436507717944 1103 80-81 2.793582336776854 1103 82-83 1.4260671435052394 1103 84-85 0.9143724165990612 1103 86-87 0.8229922955658573 1103 88-89 0.8052621311143575 1103 90-91 2.012314271024973 1103 92-93 1.8264382119259182 1103 94-95 -6.4819673560321895 1103 96-97 -7.932266170841956 1103 98-99 -7.290395618576293 1103 100-101 -9.240487121001841 1104 1 0.1702132569380268 1104 2 0.2835385336222025 1104 3 0.24211253939744992 1104 4 0.20962150426230863 1104 5 0.23807934087147942 1104 6 0.29206894504719116 1104 7 0.35087446472124384 1104 8 0.34522518073803354 1104 9 0.6040736455532993 1104 10-11 0.34724297630226886 1104 12-13 0.31455113490684283 1104 14-15 0.4618751260890619 1104 16-17 0.2728341214379597 1104 18-19 0.2896959128162635 1104 20-21 0.3293364026141177 1104 22-23 0.34456466580186174 1104 24-25 0.3586273016599293 1104 26-27 0.36381091626982 1104 28-29 0.460474658838379 1104 30-31 0.5066421428106196 1104 32-33 0.47252619048823874 1104 34-35 0.3385014345781201 1104 36-37 0.40015673587914335 1104 38-39 0.471060457646324 1104 40-41 0.42948993778808386 1104 42-43 0.2928619049442176 1104 44-45 0.43729779856811035 1104 46-47 0.6431405034471922 1104 48-49 0.5960003505397538 1104 50-51 0.5080420251009272 1104 52-53 0.3012069808201616 1104 54-55 0.3530337736969571 1104 56-57 0.6889346973342043 1104 58-59 -8.452403636769823 1104 60-61 -6.985123120473688 1104 62-63 -1.8894496017555475 1104 64-65 -0.2627777671673144 1104 66-67 -1.3591074468647832 1104 68-69 -3.4591600812536676 1104 70-71 -3.8214371856484455 1104 72-73 -3.1924211481428095 1104 74-75 -3.4097921452171995 1104 76-77 -0.029717496898534534 1104 78-79 3.608137743242505 1104 80-81 2.9699190279818097 1104 82-83 1.2869791500487864 1104 84-85 1.0160933258073754 1104 86-87 0.9051256202892617 1104 88-89 0.9468147162450187 1104 90-91 2.5611002712832693 1104 92-93 2.1362126318320698 1104 94-95 -6.053445895788528 1104 96-97 -7.70289232780093 1104 98-99 -5.502307452921292 1104 100-101 -5.037793290014713 1105 1 0.059735317077954875 1105 2 0.13962306466967433 1105 3 0.26311639929533115 1105 4 0.25459934028467757 1105 5 0.19652635858118117 1105 6 0.2313149401345953 1105 7 0.13756139764208797 1105 8 0.2506666341793604 1105 9 0.26559497524873166 1105 10-11 0.2274787236830491 1105 12-13 0.20274747409089144 1105 14-15 0.3771722093239376 1105 16-17 0.32271291778000943 1105 18-19 0.4267097554556045 1105 20-21 0.45635139104874867 1105 22-23 0.526824890234515 1105 24-25 0.5563199563960097 1105 26-27 0.3857931779264945 1105 28-29 0.37506904730595636 1105 30-31 0.2360688512988176 1105 32-33 0.06436526353984817 1105 34-35 0.23745571734608006 1105 36-37 0.19034371534792882 1105 38-39 0.14393679768971168 1105 40-41 0.27549326059441626 1105 42-43 0.33551323943766675 1105 44-45 0.40155793706611576 1105 46-47 0.19760069064150088 1105 48-49 0.19203231822832834 1105 50-51 0.16755284212458577 1105 52-53 0.08480742768031746 1105 54-55 0.2555212236579294 1105 56-57 0.6718319123355272 1105 58-59 -7.850684764805283 1105 60-61 -6.861793475168945 1105 62-63 -1.8080077258022094 1105 64-65 -0.050474432186899065 1105 66-67 -1.5108796699224811 1105 68-69 -3.0183905364924577 1105 70-71 -3.492883071885295 1105 72-73 -2.81367582979075 1105 74-75 -3.607682628671199 1105 76-77 -0.04653114761716637 1105 78-79 3.959730398694699 1105 80-81 3.3903659239507604 1105 82-83 1.8845561510728395 1105 84-85 1.6350653684023158 1105 86-87 1.404271644737669 1105 88-89 1.3357495562991524 1105 90-91 2.758020409351605 1105 92-93 1.867693715467965 1105 94-95 -5.832814956451228 1105 96-97 -7.364908676867465 1105 98-99 -5.118655803334136 1105 100-101 -4.1692652430943955 1106 1 0.13289383478714356 1106 2 0.14728553054831295 1106 3 0.16754713271043187 1106 4 0.18983793540405003 1106 5 0.11896352975302449 1106 6 0.1979193001705113 1106 7 0.32495885503167443 1106 8 0.12333080501021954 1106 9 0.25665602682041566 1106 10-11 0.26224680554932434 1106 12-13 0.2774507142142184 1106 14-15 0.14983870103215224 1106 16-17 0.2135004628809014 1106 18-19 0.21621108817480206 1106 20-21 0.24510870404404983 1106 22-23 0.35645106830293827 1106 24-25 0.22108127201212113 1106 26-27 0.22846670367841426 1106 28-29 0.1984426924937992 1106 30-31 0.3821221100784271 1106 32-33 0.49261400089330465 1106 34-35 0.6226276318735202 1106 36-37 0.34756753540494856 1106 38-39 0.6419930628981589 1106 40-41 0.33848499648571817 1106 42-43 0.41119481863403706 1106 44-45 0.51166125181458 1106 46-47 0.3244750222734538 1106 48-49 0.44207248725960824 1106 50-51 0.4251099758767438 1106 52-53 0.5052245429011677 1106 54-55 0.3215421508305738 1106 56-57 0.9196310957437603 1106 58-59 -7.423551833876381 1106 60-61 -6.961398836752707 1106 62-63 -1.7936200714415484 1106 64-65 0.022312298821628218 1106 66-67 0.9320504020425702 1106 68-69 -1.8746228955928856 1106 70-71 -2.2200027916661327 1106 72-73 -0.82184576443127 1106 74-75 -0.7490044218881664 1106 76-77 2.2537372707763232 1106 78-79 4.346878183238726 1106 80-81 3.55145445113984 1106 82-83 1.9153979332924216 1106 84-85 1.45583382875013 1106 86-87 1.4056213241888003 1106 88-89 1.4343591722296587 1106 90-91 2.6258683133315515 1106 92-93 2.6873802899065353 1106 94-95 -5.139594774815794 1106 96-97 -6.528059660651673 1106 98-99 -4.251751425266786 1106 100-101 -3.91954955057137 1107 1 0.02429126569411011 1107 2 -0.03816594341093449 1107 3 -0.12614818374494163 1107 4 0.01700549634305304 1107 5 -0.043758416427806424 1107 6 -0.15677480272041322 1107 7 -0.09082562406423733 1107 8 0.09532723300920765 1107 9 -0.00893903954401054 1107 10-11 -0.07177599521494216 1107 12-13 -0.07062602783591387 1107 14-15 0.01082960057089366 1107 16-17 0.14339739894870007 1107 18-19 0.1151048872929934 1107 20-21 0.2391579131918391 1107 22-23 -0.01462361043322602 1107 24-25 0.08121606162278994 1107 26-27 0.25028669416325755 1107 28-29 0.1780054531112114 1107 30-31 0.06025657912490345 1107 32-33 0.04351119900960754 1107 34-35 -0.011370744952230893 1107 36-37 -0.06265286519852253 1107 38-39 0.08936837996413516 1107 40-41 -0.08198073749179713 1107 42-43 -0.12532151360276345 1107 44-45 -0.11485856592178578 1107 46-47 -0.002361797831518686 1107 48-49 -0.10284084592107945 1107 50-51 -1.5277227428356355E-4 1107 52-53 0.17180725227920135 1107 54-55 -0.09156459958984442 1107 56-57 0.4894199558836405 1107 58-59 -8.12369964958759 1107 60-61 -7.280767274409335 1107 62-63 -2.165137434063258 1107 64-65 -0.4997023199232373 1107 66-67 0.3844262661029987 1107 68-69 -2.748957915704473 1107 70-71 -2.69019413795942 1107 72-73 -1.2153561204356187 1107 74-75 -1.0412724644367017 1107 76-77 2.0323902396841405 1107 78-79 4.107321325073116 1107 80-81 3.0994093279134383 1107 82-83 1.4944284555830798 1107 84-85 1.035242158839658 1107 86-87 0.8445805591089233 1107 88-89 0.6809302802368897 1107 90-91 2.0302485940541963 1107 92-93 1.4943472183672917 1107 94-95 -6.38261932341816 1107 96-97 -8.181817258150254 1107 98-99 -6.13736597989881 1107 100-101 -5.616331442256291 1108 1 -0.2664431151827209 1108 2 -0.21743335278561204 1108 3 -0.11251763581301333 1108 4 -0.09392029362595622 1108 5 -0.21754913438435608 1108 6 -0.2623922506101337 1108 7 -0.3717637158532199 1108 8 -0.33007840677576183 1108 9 -0.16060454829570858 1108 10-11 -0.3637799764715055 1108 12-13 -0.2642351584470788 1108 14-15 -0.417798902532482 1108 16-17 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-0.30327290295598885 2312 34-35 -0.5092164755928081 2312 36-37 -0.5514250184657143 2312 38-39 -0.5660921404852459 2312 40-41 -0.3536558042307476 2312 42-43 -0.5483000711260928 2312 44-45 -0.6082016368682659 2312 46-47 -0.6889274426800895 2312 48-49 -0.4744049638424599 2312 50-51 -0.3884475931108753 2312 52-53 -0.29197774374762986 2312 54-55 -0.2611813527034812 2312 56-57 0.5127965403171828 2312 58-59 3.380704765954402 2312 60-61 3.23582178332272 2312 62-63 0.5973950082074531 2312 64-65 0.28385160901428463 2312 66-67 -1.848422632812845 2312 68-69 -4.5704360601289 2312 70-71 -4.774577184411939 2312 72-73 -7.083224048730802 2312 74-75 -7.192472976411048 2312 76-77 -4.96741090468268 2312 78-79 -0.11518865913679832 2312 80-81 1.345368889200035 2312 82-83 0.44192583376625194 2312 84-85 0.15520497599269234 2312 86-87 0.21936384986979007 2312 88-89 0.5201681265672562 2312 90-91 2.0114117574685473 2312 92-93 3.401328944955001 2312 94-95 5.144633367643042 2312 96-97 0.28158501969367755 2312 98-99 -0.575381021907063 2312 100-101 0.2496497248526719 2313 1 -0.3375873203160822 2313 2 -0.3367737172457943 2313 3 -0.22540634175783936 2313 4 -0.18094344984046273 2313 5 -0.20025306508068041 2313 6 -0.14298590060079164 2313 7 -0.28037844351357677 2313 8 -0.29649411887766774 2313 9 -0.4958378464891169 2313 10-11 -0.42369956836658673 2313 12-13 -0.24944182675815085 2313 14-15 -0.47103942143026956 2313 16-17 -0.27447167355548885 2313 18-19 -0.40297322035893046 2313 20-21 -0.5619907937336208 2313 22-23 -0.5132059749371791 2313 24-25 -0.4412218768755807 2313 26-27 -0.3719704593130544 2313 28-29 -0.5943494315959299 2313 30-31 -0.6294595860231169 2313 32-33 -0.6677385849716231 2313 34-35 -0.6043511411010627 2313 36-37 -0.8229280592997767 2313 38-39 -0.8632250683740423 2313 40-41 -0.7359320857251035 2313 42-43 -0.7407414264692775 2313 44-45 -0.9744049383452378 2313 46-47 -0.9934452532795746 2313 48-49 -1.020451879567922 2313 50-51 -0.9653372542750844 2313 52-53 -0.6025772224617967 2313 54-55 -0.7729102840675068 2313 56-57 -0.2978029383969769 2313 58-59 2.6065952959283365 2313 60-61 2.699331774634622 2313 62-63 0.2216347996931205 2313 64-65 -0.27001459428719343 2313 66-67 -2.0621498265574125 2313 68-69 -4.566092011475554 2313 70-71 -3.757635394663893 2313 72-73 -2.9355263945170726 2313 74-75 -3.662064635837634 2313 76-77 0.21026068523912755 2313 78-79 2.4877652939474757 2313 80-81 1.7298171950210595 2313 82-83 0.49709525166373325 2313 84-85 0.010548155836307416 2313 86-87 0.0360449966986387 2313 88-89 0.09922981205813386 2313 90-91 1.6291354759741914 2313 92-93 3.1923802047291083 2313 94-95 5.453929631761202 2313 96-97 1.8324103889378112 2313 98-99 3.057981271750627 2313 100-101 3.8930033304130536 2314 1 -0.44911674722343875 2314 2 -0.2785366501758304 2314 3 -0.5302949670924519 2314 4 -0.37780393615938834 2314 5 -0.30831108379609873 2314 6 -0.2937810836867314 2314 7 -0.2493227094459698 2314 8 -0.48746907049554267 2314 9 -0.5192543373252434 2314 10-11 -0.5356316050741867 2314 12-13 -0.6269480823414355 2314 14-15 -0.7433296774303457 2314 16-17 -0.777006851155825 2314 18-19 -0.6207533272209886 2314 20-21 -0.5358073660877025 2314 22-23 -0.5420601880514582 2314 24-25 -0.7091500797878538 2314 26-27 -0.6150077231293452 2314 28-29 -0.636524627767642 2314 30-31 -0.7300783256514194 2314 32-33 -0.7357551245346485 2314 34-35 -0.48990997576213147 2314 36-37 -0.7106075864956622 2314 38-39 -0.8031775979587721 2314 40-41 -0.5927838073321041 2314 42-43 -0.7387429342448897 2314 44-45 -0.7623038897976286 2314 46-47 -0.4824292209052956 2314 48-49 -0.3950475459388585 2314 50-51 -0.6952603944936229 2314 52-53 -0.6908020158704815 2314 54-55 -0.7378001045901144 2314 56-57 0.21289284488785398 2314 58-59 3.1095947580937384 2314 60-61 3.0523474024837256 2314 62-63 0.5015869002360098 2314 64-65 -0.06983937666338136 2314 66-67 -1.994679549197187 2314 68-69 -4.8869685746052305 2314 70-71 -3.778670703275221 2314 72-73 -2.6844871172687057 2314 74-75 -3.7413791495570123 2314 76-77 0.18523950129816313 2314 78-79 2.849497749583783 2314 80-81 2.1137793811118293 2314 82-83 0.6320438509187305 2314 84-85 0.3883735112898137 2314 86-87 0.44188027221245463 2314 88-89 0.3948063140493474 2314 90-91 1.9892877551823638 2314 92-93 3.596629174645617 2314 94-95 6.000928101001197 2314 96-97 3.3966731954537313 2314 98-99 5.543196183406312 2314 100-101 5.931835447872453 2315 1 -0.41416080796548016 2315 2 -0.3453214624976617 2315 3 -0.5214309460832744 2315 4 -0.3389188409383266 2315 5 -0.37932931972507333 2315 6 -0.4378792978756678 2315 7 -0.36251414411823646 2315 8 -0.6504055552055092 2315 9 -0.6563838159823803 2315 10-11 -0.6231093269534256 2315 12-13 -0.6852010131433843 2315 14-15 -0.9262026607862097 2315 16-17 -0.8108037861819071 2315 18-19 -0.6689987171403686 2315 20-21 -0.7352695168960608 2315 22-23 -0.8676525034740337 2315 24-25 -0.5460076531101663 2315 26-27 -0.5826945767541574 2315 28-29 -0.6734652746731769 2315 30-31 -0.4693976022841255 2315 32-33 -0.722821427009201 2315 34-35 -0.5679252061771223 2315 36-37 -0.40272163498958236 2315 38-39 -0.719929815706017 2315 40-41 -0.7887151372991426 2315 42-43 -0.5619367225396488 2315 44-45 -0.37125609422831474 2315 46-47 -0.6409220818418291 2315 48-49 -0.4124364805065994 2315 50-51 -0.5719216726035441 2315 52-53 -0.5805022851434813 2315 54-55 -0.5131220631137055 2315 56-57 0.3276191665939834 2315 58-59 3.029428363177562 2315 60-61 3.1809635808511487 2315 62-63 0.5515727320480046 2315 64-65 0.1422657528400535 2315 66-67 -1.6413919102057157 2315 68-69 -4.264020445657689 2315 70-71 -3.4150360288416017 2315 72-73 -2.6975652064369307 2315 74-75 -0.25894073472175805 2315 76-77 2.054474481083332 2315 78-79 3.6170817870683187 2315 80-81 2.571187717580745 2315 82-83 0.84026937440618 2315 84-85 0.6844596074198037 2315 86-87 0.6672543356023191 2315 88-89 0.5243294887144607 2315 90-91 2.0947633340263963 2315 92-93 3.8021008049782345 2315 94-95 6.287248599349791 2315 96-97 4.323139525490445 2315 98-99 6.2562617302441765 2315 100-101 6.602058097462967 2316 1 -0.5479366615773351 2316 2 -0.38849818570370687 2316 3 -0.5354310871153025 2316 4 -0.43769627922325327 2316 5 -0.7019757315060531 2316 6 -0.5205899334729267 2316 7 -0.40820619680842185 2316 8 -0.4462361214956019 2316 9 -0.5314007669474066 2316 10-11 -0.5597909827934302 2316 12-13 -0.5126762221148837 2316 14-15 -0.7942048181113108 2316 16-17 -1.0944835132926798 2316 18-19 -0.7752190820103664 2316 20-21 -0.7643709336480313 2316 22-23 -1.0761555724704621 2316 24-25 -0.8679884247103473 2316 26-27 -0.5361559444393791 2316 28-29 -0.761754036977635 2316 30-31 -0.712675915045125 2316 32-33 -0.8823096667202535 2316 34-35 -0.9271810530008437 2316 36-37 -0.6910457327914159 2316 38-39 -0.7475410973384626 2316 40-41 -0.8181737835311438 2316 42-43 -1.1847062661305543 2316 44-45 -1.099091787265131 2316 46-47 -1.2778355106481598 2316 48-49 -1.1898743779825196 2316 50-51 -1.050155858612385 2316 52-53 -0.96778902263096 2316 54-55 -0.9409613206963101 2316 56-57 -0.22164789305093535 2316 58-59 2.8401877896576444 2316 60-61 2.446346726903762 2316 62-63 0.0752967215186473 2316 64-65 -0.5172511582175545 2316 66-67 -2.268359093912327 2316 68-69 -2.8054218700811813 2316 70-71 -2.9360722732981515 2316 72-73 -0.6373739763044313 2316 74-75 5.144411255650866 2316 76-77 5.386641329722643 2316 78-79 4.324205919275773 2316 80-81 2.512016257504044 2316 82-83 0.9278657624364364 2316 84-85 0.4396105698084227 2316 86-87 0.3314299530242728 2316 88-89 0.415013393317917 2316 90-91 1.8780544481561812 2316 92-93 3.3955643578850037 2316 94-95 5.862778224987245 2316 96-97 3.137936187525783 2316 98-99 5.260110277343063 2316 100-101 5.33335643677208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 28.0 5 75.0 6 195.0 7 413.0 8 776.0 9 1321.0 10 2238.0 11 3513.0 12 4829.0 13 6557.0 14 7946.0 15 10377.0 16 13470.0 17 17363.0 18 17622.0 19 15751.0 20 16080.0 21 16416.0 22 16317.0 23 17236.0 24 17690.0 25 18458.0 26 22803.0 27 31495.0 28 43509.0 29 57901.0 30 69091.0 31 76617.0 32 79647.0 33 81467.0 34 83063.0 35 88163.0 36 85555.0 37 64172.0 38 25052.0 39 1591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39474768422235 21.168937925922712 15.062066192110487 21.374248197744443 2 15.200864126717562 23.377249262318834 38.62926322553432 22.792623385429295 3 18.846515037894054 27.76824664491532 31.866874654489592 21.518363662701038 4 11.944734318939464 18.486963530996533 39.05038100840849 30.517921141655506 5 11.657761076440828 39.63449446190284 35.05532180914789 13.65242265250844 6 28.032522440227552 39.337332123251024 17.74613300854251 14.884012427978915 7 24.71662099295728 35.48576231736675 22.90809976675368 16.88951692292229 8 24.909490089731438 34.13105559092083 21.160202048470346 19.79925227087739 9 25.719176158619323 17.000572522657016 22.531870592142152 34.74838072658151 10-11 23.56384021789365 27.301004033279234 28.53113941862837 20.604016330198746 12-13 25.605410684001985 25.949958957713143 28.549714379187375 19.8949159790975 14-15 22.352417168652437 26.785149432944127 28.583133869332272 22.27929952907116 16-17 19.676647874916117 29.959095670408264 29.219004204740408 21.145252249935208 18-19 19.4528300306414 28.70851352010512 32.09596734737316 19.742689101880316 20-21 20.717338370744386 27.30051132875512 31.658335033163944 20.32381526733655 22-23 20.317311567618276 27.43723683419606 31.705339044764173 20.540112553421487 24-25 20.815298285929387 27.87960069234874 31.567643683795875 19.737457337926 26-27 20.345799913579583 27.372459675810116 32.645780254659385 19.63596015595091 28-29 19.551131237771553 28.464799065427464 32.254429587044875 19.7296401097561 30-31 20.08400094407443 27.769781833071285 31.791903527024623 20.354313695829664 32-33 20.031209688307158 28.081870492111843 31.866451703820353 20.020468115760647 34-35 20.742302460069265 27.713508775473905 31.65132438926929 19.89286437518754 36-37 20.620852631828114 27.498629050711315 31.957814213686607 19.92270410377396 38-39 20.133743673902526 28.171481067444347 31.827414346501744 19.867360912151383 40-41 19.92613648131911 28.274568324001354 31.92005696831138 19.879238226368162 42-43 19.779635029764854 28.536435131553706 30.944451595308443 20.739478243373 44-45 20.205715063528924 27.89305302321544 30.889168247718178 21.01206366553745 46-47 20.718547282269384 28.547834603537503 30.50936058444844 20.224257529744673 48-49 20.414733682059534 28.533663518663165 30.34830212943896 20.703300669838338 50-51 19.66816629061016 28.756045174116796 30.911894299193715 20.663894236079337 52-53 20.3197474914805 29.303580162186037 29.736112425848795 20.640559920484666 54-55 19.865380946582736 28.516927700182247 29.997007698644197 21.620683654590817 56-57 20.43050143650037 28.684745485266934 29.588505630381327 21.296247447851364 58-59 19.987982808767885 28.13059739630831 30.931317435446047 20.950102359477754 60-61 19.864360055925026 28.78121805025738 30.95859609988194 20.395825793935654 62-63 20.127082107694598 28.888465150471482 30.19525472098796 20.78919802084596 64-65 19.971284472921276 29.360049744674228 29.946234757810053 20.722431024594446 66-67 20.395647223027517 28.555310812305173 29.27260446335974 21.776437501307566 68-69 20.002603369143113 28.36779349833783 30.133089679508107 21.496513453010955 70-71 21.1149903825345 28.489490402964996 28.989154687083037 21.40636452741747 72-73 20.442259185012613 28.100650360497575 29.88743606259996 21.56965439188986 74-75 19.77573242806479 29.451039538289088 29.402329116754288 21.370898916891836 76-77 18.73117061501506 28.977483418723825 30.162062397522725 22.12928356873839 78-79 19.849305497053997 29.003414288475167 29.3537629026903 21.793517311780533 80-81 19.98757898680021 29.06186847527134 29.098195009907236 21.852357528021216 82-83 19.965894942429856 28.912092021574576 29.20111292262409 21.920900113371477 84-85 19.751063267888323 29.007702040607175 28.881371231262243 22.359863460242256 86-87 19.622612876412788 29.279799289214996 28.859566010227695 22.238021824144518 88-89 19.712486918681996 28.925282270700016 28.555211537968834 22.807019272649157 90-91 19.823286565359403 28.440352912736312 28.89738746296636 22.83897305893793 92-93 19.77814498366306 29.12771403162484 28.90316741367649 22.190973571035606 94-95 19.122923343462414 28.69955625370887 29.26019569405432 22.917324708774398 96-97 18.668505541673262 28.85868182118761 28.858982400102594 23.61383023703653 98-99 19.114617786985804 28.793393626295376 28.640909095417133 23.451079491301687 100-101 18.132805207005834 28.730197740168983 29.38810942798647 23.74888762483872 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3525.0 1 2478.0 2 1424.0 3 1448.5 4 1445.0 5 1576.0 6 2053.0 7 2812.5 8 3663.5 9 4120.5 10 3941.0 11 3597.0 12 3402.0 13 3294.5 14 3211.0 15 3169.0 16 3284.0 17 3371.0 18 3462.5 19 3693.5 20 3959.5 21 4249.5 22 4713.5 23 5285.5 24 5877.0 25 6930.5 26 8369.0 27 9850.0 28 11098.0 29 12417.5 30 14371.0 31 16583.5 32 19153.5 33 22056.0 34 24882.5 35 27666.5 36 30654.5 37 33016.0 38 33783.5 39 33464.5 40 32914.5 41 32126.0 42 31192.0 43 31053.0 44 31930.0 45 33545.5 46 35856.5 47 39151.0 48 41948.5 49 39586.0 50 33674.5 51 29285.0 52 26329.5 53 24432.0 54 23133.0 55 21732.5 56 20232.5 57 18655.5 58 16906.5 59 14826.5 60 12513.0 61 10042.5 62 7874.5 63 6012.5 64 4333.0 65 3104.5 66 2211.5 67 1542.0 68 1031.0 69 672.0 70 431.0 71 302.0 72 213.5 73 159.0 74 123.0 75 73.0 76 34.0 77 16.5 78 6.0 79 3.5 80 3.0 81 1.0 82 1.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02414252168146258 2 0.013894267579943774 3 3.94163619289185E-4 4 0.0 5 0.06405158813449256 6 0.0 7 0.0 8 9.854090482229625E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 3.94163619289185E-4 16-17 0.0 18-19 3.448931668780369E-4 20-21 0.0 22-23 0.0 24-25 4.927045241114812E-5 26-27 4.927045241114812E-5 28-29 0.0011332204054564068 30-31 0.005567561122459739 32-33 0.0057153724796931826 34-35 0.001823006739212481 36-37 0.08977076429311188 38-39 0.08400612136100756 40-41 0.0896722233882896 42-43 0.09297334369983652 44-45 0.0963237344637946 46-47 0.09144595967509092 48-49 0.08188749190732819 50-51 0.0763692012372796 52-53 0.06533261989718242 54-55 0.05296573634198424 56-57 0.07001331287624149 58-59 0.6980637697611467 60-61 0.340261744351389 62-63 0.0029562271446688876 64-65 0.0024635226205574064 66-67 1.087300343809217 68-69 2.343647609841083 70-71 2.329654801356317 72-73 1.786448063523409 74-75 1.2778784537355379 76-77 0.7461024608620161 78-79 0.5727197388271859 80-81 0.03912073921445162 82-83 1.478113572334444E-4 84-85 0.0010839499530452589 86-87 0.0013303022151009995 88-89 9.854090482229625E-5 90-91 0.33459564232410693 92-93 0.05331062950886228 94-95 0.9475200703187896 96-97 1.6488849603914835 98-99 0.641304208583504 100-101 0.5621758620112002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1014807.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.32037868413583 #Duplication Level Percentage of deduplicated Percentage of total 1 69.34589130442772 27.2670670627902 2 14.060270163535698 11.057102944629602 3 5.9371934647383755 7.003580860634681 4 3.118068867266714 4.904145944965666 5 1.8158469312013144 3.5699894483630814 6 1.191602681040308 2.811256119572186 7 0.8014100180912894 2.205822177482675 8 0.581102537083765 1.8279337449956583 9 0.4339105591851309 1.535537475198403 >10 2.4192855636528936 17.385953339582013 >50 0.1865157467771042 5.091153585297711 >100 0.09863782121287952 6.915402315464632 >500 0.0035048971954969865 0.9657800182133037 >1k 0.005758045392602192 4.437902411692017 >5k 7.510493990350685E-4 2.031770418501946 >10k+ 2.5034979967835617E-4 0.9896021326162818 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10053 0.9906317161785442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7470 0.736100559022553 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7250 0.7144215599616479 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5920 0.5833621565479938 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4905 0.4833431381533631 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4376 0.43121499950236847 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4038 0.39790817367243225 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3201 0.3154294363361703 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2598 0.2560092707283257 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1952 0.1923518462131223 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.19116935535525476 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1920 0.1891985372588088 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1906 0.18781896459129668 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1806 0.17796487410906703 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1605 0.1581581522397855 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1531 0.15086612528293555 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1527 0.1504719616636464 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1526 0.1503734207588241 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1348 0.13283313970045538 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1188 0.11706659492888795 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1170 0.11529285864208662 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1147 0.11302641783117381 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1140 0.11233663149741774 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1136 0.11194246787812855 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1061 0.10455190001645633 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1048 0.10327086825376648 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.956227144668888E-4 0.0 18-19 0.0 0.0 0.0 5.419749765226294E-4 0.0 20-21 0.0 0.0 0.0 0.001133220405456407 0.0 22-23 0.0 0.0 0.0 0.0018722771916236287 0.0 24-25 0.0 0.0 0.0 0.0029069566922577394 0.0 26-27 0.0 0.0 0.0 0.005666102027282035 0.0 28-29 0.0 0.0 0.0 0.014091349389588366 0.0 30-31 0.0 0.0 0.0 0.03409515306851451 0.0 32-33 0.0 0.0 0.0 0.07341297409261072 0.0 34-35 0.0 0.0 0.0 0.13238970562875502 0.0 36-37 0.0 0.0 0.0 0.21984475865854294 0.0 38-39 0.0 0.0 0.0 0.34646982135519366 0.0 40-41 0.0 0.0 0.0 0.472996343147022 0.0 42-43 0.0 0.0 0.0 0.6077510304915121 0.0 44-45 0.0 0.0 0.0 0.7656135600168308 0.0 46-47 0.0 0.0 0.0 0.9507719201779254 0.0 48-49 0.0 0.0 0.0 1.1226765286404214 0.0 50-51 0.0 0.0 0.0 1.3020702458694116 0.0 52-53 0.0 0.0 0.0 1.4754036974518308 0.0 54-55 0.0 0.0 0.0 1.6636660961148277 0.0 56-57 0.0 0.0 0.0 1.8535544197073928 0.0 58-59 0.0 0.0 0.0 1.9852050685499805 0.0 60-61 0.0 0.0 0.0 2.099808140858311 0.0 62-63 0.0 0.0 0.0 2.197905611608907 0.0 64-65 0.0 0.0 0.0 2.303640002483231 0.0 66-67 0.0 0.0 0.0 2.399273950613269 0.0 68-69 0.0 0.0 0.0 2.5094426822045968 0.0 70-71 0.0 0.0 0.0 2.673907452353009 0.0 72-73 0.0 0.0 0.0 2.8952303245838866 0.0 74-75 0.0 0.0 0.0 3.197159656959402 0.0 76-77 0.0 0.0 0.0 3.5565876072987277 0.0 78-79 0.0 0.0 0.0 4.043034783953993 0.0 80-81 0.0 0.0 0.0 4.610728936635242 0.0 82-83 0.0 0.0 0.0 5.132256675407245 0.0 84-85 0.0 0.0 0.0 5.624961199518726 4.927045241114813E-5 86-87 0.0 0.0 0.0 6.069971925696216 9.854090482229626E-5 88-89 0.0 0.0 0.0 6.488672230286153 9.854090482229626E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5215 0.0 27.13391 1 TACATGG 5385 0.0 25.047333 2 GTCGGGA 170 1.2031524E-7 24.967482 2 ACATGGG 5125 0.0 24.659216 3 CATGGGA 3700 0.0 24.34277 4 ATGGGAT 1540 0.0 23.884283 5 GGATATG 445 0.0 22.25895 1 GAGTACT 6315 0.0 21.753305 12-13 AGTACTT 6545 0.0 21.456882 12-13 AGGACCG 115 0.0038830093 20.502672 5 CTTTGCG 300 1.8371793E-10 20.436346 2 AGTACAT 3895 0.0 19.978258 2 GTACTTT 6885 0.0 19.849709 14-15 TCGACGA 60 0.0048973807 19.697926 84-85 TTTGCGT 290 2.6684575E-9 19.512888 3 CGGATAG 75 6.56821E-4 19.463186 66-67 AGAGTAC 10065 0.0 18.939337 10-11 GGACCGA 125 0.0063141757 18.84945 6 CGCATAG 325 0.0 18.714603 66-67 ACTTTTT 7695 0.0 18.065586 16-17 >>END_MODULE