FastQCFastQC Report
Fri 27 May 2016
SRR939699_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939699_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551274
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72371.3127773121895827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43460.7883556997064981No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42410.7693089099068703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.7128941325003537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.6677260309755222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.27100860914898944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.2557711773092872No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.16616056625199085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.1618070142977902No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.15690926834931448No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.10212707292562319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44350.039.9037631
GGTATCA24800.039.2193951
TATCAAC54700.032.2666782
ATCAACG56950.031.4084343
TCAACGC56950.031.2418124
CAACGCA57600.030.8892565
AACGCAG59250.030.0263216
GGGTCGC350.00838973327.14397494-95
ACGCAGA65500.026.5093467
CGCAGAG65850.025.9361768
CTACACT2101.2787496E-924.8526134
GCAGAGT64750.024.5495269
GTACATG21550.023.3376561
GTATAGA1252.2712903E-422.774031
AGTACTT43400.022.74317612-13
GAGTACT41900.022.53806312-13
TACATGG21500.022.5092142
CATATAG1302.96155E-421.8981063
GTATCAC1100.002894562221.5663171
AGAGTAC56250.021.55391510-11
Produced by FastQC (version 0.11.5)