##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939699_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 551274 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.658565432071892 31.0 31.0 33.0 27.0 34.0 2 30.79829268204196 31.0 31.0 34.0 27.0 34.0 3 30.832429971302837 31.0 31.0 34.0 27.0 34.0 4 34.475872252273824 37.0 35.0 37.0 30.0 37.0 5 34.473619289137524 37.0 35.0 37.0 30.0 37.0 6 34.62418325551359 37.0 35.0 37.0 31.0 37.0 7 34.4692494113635 37.0 35.0 37.0 30.0 37.0 8 34.48712074213549 37.0 35.0 37.0 30.0 37.0 9 35.808119011598585 38.0 35.0 39.0 30.0 39.0 10-11 35.75332411831503 38.0 35.0 39.0 30.0 39.0 12-13 35.607500988619094 38.0 35.0 39.0 30.0 39.0 14-15 36.312526075962225 38.0 35.5 40.0 29.5 41.0 16-17 36.06960422584776 38.0 35.0 40.0 28.5 41.0 18-19 36.08460584028995 38.0 35.0 40.0 29.0 41.0 20-21 36.18060800981 39.0 35.0 40.0 29.0 41.0 22-23 35.991752740016764 38.5 35.0 40.0 27.5 41.0 24-25 35.849183890406586 38.0 35.0 40.0 27.0 41.0 26-27 35.87327082358319 38.0 35.0 40.0 27.5 41.0 28-29 35.67693742131862 38.0 35.0 40.0 27.0 41.0 30-31 35.3546004346296 38.0 34.0 40.0 27.0 41.0 32-33 35.00792165057666 38.0 34.0 40.0 25.5 41.0 34-35 34.7196865805389 38.0 34.0 40.0 25.0 41.0 36-37 34.37082557856891 38.0 33.0 40.0 24.0 41.0 38-39 34.15925474446465 38.0 33.0 40.0 23.5 41.0 40-41 33.825288332117964 37.5 33.0 40.0 22.0 41.0 42-43 33.41499599110424 37.0 33.0 40.0 20.0 41.0 44-45 33.04181223856014 37.0 32.5 40.0 18.0 41.0 46-47 32.45779866273396 37.0 31.0 40.0 12.0 41.0 48-49 32.00806223402519 36.5 31.0 39.5 8.5 41.0 50-51 31.3478705689004 35.5 30.0 38.5 7.5 40.5 52-53 31.386518319383825 36.0 30.0 39.0 6.0 40.0 54-55 31.43302695211456 36.0 30.0 39.0 2.0 40.0 56-57 31.29856205806913 36.0 30.0 39.0 2.0 40.0 58-59 30.910749463968916 35.0 29.5 39.0 2.0 40.0 60-61 30.50508186491654 35.0 28.5 39.0 2.0 40.0 62-63 30.077071474439208 35.0 28.0 38.0 2.0 40.0 64-65 29.47174635480723 34.5 27.5 38.0 2.0 40.0 66-67 28.918360016978852 34.0 26.0 37.5 2.0 39.5 68-69 28.384154703468692 34.0 26.0 37.0 2.0 39.0 70-71 27.813871142118074 33.5 25.5 36.0 2.0 39.0 72-73 27.061736994670525 33.0 23.5 36.0 2.0 38.5 74-75 26.614335158197193 32.0 23.0 35.0 2.0 38.0 76-77 26.247343426317947 32.0 22.0 35.0 2.0 37.0 78-79 25.656500215863616 32.0 20.0 35.0 2.0 36.5 80-81 24.955796028835024 31.0 18.0 34.5 2.0 36.0 82-83 24.252246614206364 31.0 10.5 34.0 2.0 35.5 84-85 23.579481346843856 30.5 6.0 34.0 2.0 35.0 86-87 22.860443264148138 30.0 2.0 34.0 2.0 35.0 88-89 22.021515616553657 29.0 2.0 34.0 2.0 35.0 90-91 20.756795169008516 28.0 2.0 33.5 2.0 35.0 92-93 19.159875125618115 25.0 2.0 33.0 2.0 34.0 94-95 18.928171834695632 25.0 2.0 32.0 2.0 34.0 96-97 18.298913063195435 25.0 2.0 32.0 2.0 34.0 98-99 17.31738028639116 22.0 2.0 32.0 2.0 34.0 100-101 15.693704945272223 9.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7858789666452495 1101 2 0.7091838749970201 1101 3 0.5170886782202047 1101 4 0.8543597408382411 1101 5 0.6191129043811472 1101 6 0.9840208654108196 1101 7 0.4600194700009155 1101 8 0.6288963159295449 1101 9 0.9622562246612176 1101 10-11 0.7680429438286893 1101 12-13 0.4727777426724984 1101 14-15 0.6424450758021081 1101 16-17 0.6028039443003692 1101 18-19 0.6446104397474599 1101 20-21 0.6838258617060475 1101 22-23 0.8976077314351656 1101 24-25 0.6375341786501281 1101 26-27 0.4291327212346161 1101 28-29 0.6593818033833827 1101 30-31 0.9594385868319364 1101 32-33 0.7622350105857834 1101 34-35 1.052241540290403 1101 36-37 0.7483496371194818 1101 38-39 0.19111521312849078 1101 40-41 0.4644077418574213 1101 42-43 0.41746688194196935 1101 44-45 0.24416932135746805 1101 46-47 0.189131744988579 1101 48-49 -0.037212007865978336 1101 50-51 0.08750125737141445 1101 52-53 -0.11785289421129619 1101 54-55 0.40769255818813477 1101 56-57 0.18689362406574972 1101 58-59 -0.08717817552266993 1101 60-61 -0.48051695925588334 1101 62-63 -0.27557467284481163 1101 64-65 -0.24289026548349568 1101 66-67 0.07711942776497338 1101 68-69 0.047142523971153594 1101 70-71 -0.561804727178906 1101 72-73 -0.1843467828522556 1101 74-75 -0.5598171669708876 1101 76-77 -1.1376400547708236 1101 78-79 -1.3831435569512784 1101 80-81 -1.3237809247124872 1101 82-83 -0.778250358570876 1101 84-85 -1.1219447604678443 1101 86-87 -1.6506905275723653 1101 88-89 -2.4299937829069123 1101 90-91 -5.274819211040461 1101 92-93 -9.472094691419827 1101 94-95 -7.8455617771806505 1101 96-97 -4.736615189897833 1101 98-99 -3.5890176784488936 1101 100-101 -2.7485158582146507 1102 1 0.8884466522347694 1102 2 0.807183874997019 1102 3 0.9150100756001187 1102 4 0.9459143259910832 1102 5 0.8397591925907548 1102 6 0.8661344025287221 1102 7 0.8757050595205627 1102 8 0.9259094163662311 1102 9 1.2436972726961528 1102 10-11 0.7374927254880816 1102 12-13 0.658183856209611 1102 14-15 1.0662049011296162 1102 16-17 1.0258563460470924 1102 18-19 1.0739947192234425 1102 20-21 0.8700966040641305 1102 22-23 1.104236552395868 1102 24-25 1.0351018642396497 1102 26-27 0.8089056469988094 1102 28-29 1.3305783099335997 1102 30-31 1.2657442636878358 1102 32-33 1.3611083730311933 1102 34-35 1.1723070424738111 1102 36-37 1.4414195061151176 1102 38-39 1.7000366105084126 1102 40-41 1.6654645104163777 1102 42-43 0.9852485413306198 1102 44-45 0.8401518541085622 1102 46-47 0.9739789065606388 1102 48-49 1.161556551085983 1102 50-51 0.6319466722185787 1102 52-53 0.8471733066620679 1102 54-55 1.0695746542580018 1102 56-57 0.7476054144587643 1102 58-59 0.500987763341957 1102 60-61 0.565334569128396 1102 62-63 0.5908008730067174 1102 64-65 0.3796119179226167 1102 66-67 0.776337768376326 1102 68-69 0.5111556244078344 1102 70-71 1.063955098148604 1102 72-73 0.6676750512088816 1102 74-75 0.26583348805094786 1102 76-77 -0.42175795870095456 1102 78-79 -0.6264841683049873 1102 80-81 -0.549558217288908 1102 82-83 -0.34475690878921483 1102 84-85 -0.6688006556643487 1102 86-87 -0.558528955519968 1102 88-89 -1.2220942195881328 1102 90-91 -4.73380611060378 1102 92-93 -10.09896805386524 1102 94-95 -8.3286665806741 1102 96-97 -4.596204709548488 1102 98-99 -3.11805697975894 1102 100-101 -2.836380487035612 1103 1 0.8034932843591243 1103 2 0.8482166901956383 1103 3 0.6305126662736953 1103 4 0.8027951550066277 1103 5 0.6770165328325533 1103 6 0.619840792856877 1103 7 0.7919537641837735 1103 8 0.6618057894232194 1103 9 0.9519010723507293 1103 10-11 0.7053614646936097 1103 12-13 0.5529886921336242 1103 14-15 0.4341824486253003 1103 16-17 0.7167389021783492 1103 18-19 0.451492128549873 1103 20-21 0.6774264831660304 1103 22-23 0.4466061551592517 1103 24-25 0.5627478055177093 1103 26-27 0.6825360442354267 1103 28-29 0.7139047348040748 1103 30-31 0.44235220842013234 1103 32-33 0.7118130362436332 1103 34-35 0.6153208593995458 1103 36-37 0.936388418032216 1103 38-39 0.8658518091267169 1103 40-41 0.5888807453041096 1103 42-43 0.7277839472028091 1103 44-45 0.3967494361465569 1103 46-47 0.5582103400666725 1103 48-49 0.5946860847647422 1103 50-51 0.38215738033602165 1103 52-53 0.6319038766102523 1103 54-55 0.6447542742925485 1103 56-57 0.5574447926971047 1103 58-59 0.37242126938686226 1103 60-61 0.054108316969504244 1103 62-63 0.12033455176319308 1103 64-65 -0.08110138086840024 1103 66-67 -0.21031335424889264 1103 68-69 -0.05336769165433708 1103 70-71 0.17509672163737733 1103 72-73 0.07861978350594612 1103 74-75 -0.3061423323290171 1103 76-77 -0.3802847289945639 1103 78-79 0.014232584717465357 1103 80-81 -0.5160469910367489 1103 82-83 -0.42682944764932174 1103 84-85 -0.5367108456470788 1103 86-87 -0.6662232560381014 1103 88-89 -1.3325588137159379 1103 90-91 -3.1145349534362516 1103 92-93 -9.624177034866968 1103 94-95 -6.218941192073064 1103 96-97 -3.652342878805829 1103 98-99 -2.832823819137177 1103 100-101 -2.4611283281409655 1104 1 0.5815473233757089 1104 2 0.4021368951312496 1104 3 0.4710906125128709 1104 4 0.5523170105548374 1104 5 0.539638387221629 1104 6 0.4253693018575788 1104 7 0.3481748581782824 1104 8 0.5974127720709248 1104 9 0.2473885478639417 1104 10-11 0.34538534293774603 1104 12-13 0.33487513137740166 1104 14-15 0.42881563938465206 1104 16-17 0.19918520510749715 1104 18-19 0.19564572593485252 1104 20-21 0.1743851946681545 1104 22-23 -0.0548775415638616 1104 24-25 0.15048441457521733 1104 26-27 0.18234188860954958 1104 28-29 0.2961890481886371 1104 30-31 0.08695902878850603 1104 32-33 0.1157929367895818 1104 34-35 -0.30987416557987757 1104 36-37 -0.4550771381801866 1104 38-39 -0.32635936264595244 1104 40-41 -0.13119320770443466 1104 42-43 0.21036934669974272 1104 44-45 0.40723239102131004 1104 46-47 0.5167305844129828 1104 48-49 0.2242545376631675 1104 50-51 -0.16414728751296437 1104 52-53 0.2101196153868976 1104 54-55 0.20195049318417801 1104 56-57 0.021310112445341645 1104 58-59 -0.12830753867146427 1104 60-61 0.3254016832223563 1104 62-63 -0.014232684040262455 1104 64-65 0.1372092333588597 1104 66-67 -0.13776290276461367 1104 68-69 0.10122944991119454 1104 70-71 0.36298865519558277 1104 72-73 0.3171515612759954 1104 74-75 -0.009126243492676878 1104 76-77 -0.12777809292914455 1104 78-79 0.12922052968158582 1104 80-81 -0.26755821728890794 1104 82-83 -0.5905287208697558 1104 84-85 -0.2551764945905255 1104 86-87 -0.4000054655870784 1104 88-89 -0.039570729655245884 1104 90-91 -1.142712150872235 1104 92-93 -4.001827114267925 1104 94-95 -1.9334585269828253 1104 96-97 -1.117674508206207 1104 98-99 -1.0709093287522258 1104 100-101 -1.2989576682436645 1105 1 0.8942244300125495 1105 2 0.6809787467918902 1105 3 0.7761040926941369 1105 4 0.48336731744407047 1105 5 0.43596432079588254 1105 6 0.28244209483641214 1105 7 0.4712948031103039 1105 8 0.5800290744858856 1105 9 0.7919878709867518 1105 10-11 0.558928622923986 1105 12-13 0.581397531423292 1105 14-15 0.4333587472834637 1105 16-17 0.7077623289530734 1105 18-19 0.3580374542661815 1105 20-21 0.6214812194487465 1105 22-23 0.44386048401980105 1105 24-25 0.6792129753507581 1105 26-27 0.4852817153748745 1105 28-29 0.3626979680532614 1105 30-31 0.5502570842006591 1105 32-33 0.7220827320055534 1105 34-35 0.47841815358491857 1105 36-37 0.4474109591065698 1105 38-39 0.57577165324345 1105 40-41 0.18883203178389607 1105 42-43 0.6189579430400229 1105 44-45 0.4786988626555697 1105 46-47 0.7911583937401261 1105 48-49 0.455453986983418 1105 50-51 0.35550222777413154 1105 52-53 0.04167758016633982 1105 54-55 0.0667285004118483 1105 56-57 0.48804131189466204 1105 58-59 0.26662878898298104 1105 60-61 0.18328328707711705 1105 62-63 0.5181513003571432 1105 64-65 0.49996234527304395 1105 66-67 0.3529787940173499 1105 68-69 -0.057707623455414137 1105 70-71 0.12098928618279459 1105 72-73 0.30229898283281287 1105 74-75 0.37265400087146006 1105 76-77 0.21925913531613972 1105 78-79 -0.3536209204417382 1105 80-81 0.16470673997604734 1105 82-83 0.20515762112531277 1105 84-85 0.2759856691219724 1105 86-87 -0.22916998116099307 1105 88-89 -0.21830789480181068 1105 90-91 -1.7796693584670287 1105 92-93 -7.367412498309685 1105 94-95 -3.3191537601612797 1105 96-97 -1.8269055642493441 1105 98-99 -1.258655270357231 1105 100-101 -0.779688179343303 1106 1 0.7352658404266528 1106 2 0.8494052772110408 1106 3 0.6645451309506711 1106 4 0.7243866507143295 1106 5 0.6110802258010892 1106 6 0.657662077805476 1106 7 0.6845316277862494 1106 8 0.7174813720857856 1106 9 0.9114094498179242 1106 10-11 0.7504226147869701 1106 12-13 0.721682011191163 1106 14-15 0.8098986280668896 1106 16-17 1.1776238737223679 1106 18-19 0.8602640919171733 1106 20-21 0.8466870099681856 1106 22-23 1.1644505745360902 1106 24-25 0.9716073992949958 1106 26-27 1.244211920061538 1106 28-29 1.1947997121476277 1106 30-31 1.194319909444289 1106 32-33 1.0691969339168068 1106 34-35 1.0320671900752885 1106 36-37 0.932980391723973 1106 38-39 0.7638484186264876 1106 40-41 1.0622984587558548 1106 42-43 1.09695333837859 1106 44-45 1.0236573891639082 1106 46-47 1.2580379471510383 1106 48-49 1.0389292448129233 1106 50-51 1.2488396611484625 1106 52-53 1.2475091000200003 1106 54-55 1.3871798203096652 1106 56-57 1.153151539920021 1106 58-59 1.6179250327146555 1106 60-61 1.8623825396080989 1106 62-63 1.5138636036340216 1106 64-65 1.5191063828672675 1106 66-67 1.8828728237268813 1106 68-69 1.9945836686882785 1106 70-71 1.8150584191818133 1106 72-73 2.126579110249473 1106 74-75 2.036401753733603 1106 76-77 1.931330602184655 1106 78-79 1.9621689682263757 1106 80-81 1.5686963952572164 1106 82-83 1.8093759325391972 1106 84-85 1.7547048793909994 1106 86-87 1.6909359891294784 1106 88-89 1.367791389267957 1106 90-91 1.369116398621312 1106 92-93 -2.3380972051567532 1106 94-95 0.2843186591782967 1106 96-97 1.5722602351009556 1106 98-99 1.3518692195030546 1106 100-101 1.860589992669187 1107 1 0.7816418984147369 1107 2 0.8339716508883619 1107 3 0.6734413149889136 1107 4 0.666786991525889 1107 5 0.6503737936094325 1107 6 0.6532922972655655 1107 7 0.6618306961928866 1107 8 0.6967820819010342 1107 9 0.7149196835620302 1107 10-11 0.6443450175084564 1107 12-13 0.7024487118972189 1107 14-15 0.8434018451024485 1107 16-17 0.9800346142983614 1107 18-19 0.7557621216003554 1107 20-21 0.49540050898772847 1107 22-23 0.6760735642804221 1107 24-25 0.7801561936114609 1107 26-27 0.6695372259461791 1107 28-29 0.8758856104429427 1107 30-31 1.0389123451818918 1107 32-33 0.6804258602977526 1107 34-35 0.6459148523210061 1107 36-37 0.7820256181694418 1107 38-39 0.6242080875882081 1107 40-41 0.7150027107559325 1107 42-43 0.4678427688348634 1107 44-45 0.4633606826314818 1107 46-47 0.5156936773585983 1107 48-49 0.8302526461623891 1107 50-51 0.5861945499774883 1107 52-53 0.8167301827231874 1107 54-55 0.9083505455992587 1107 56-57 0.2999432752397517 1107 58-59 0.5798672200482393 1107 60-61 0.5797658085171875 1107 62-63 0.3227193789489924 1107 64-65 0.04120274984112271 1107 66-67 0.21222741183982308 1107 68-69 0.5161318553076661 1107 70-71 0.010544232274238396 1107 72-73 0.16592123117492363 1107 74-75 -0.14288298053988768 1107 76-77 -0.1357087227077436 1107 78-79 -0.35954868443402077 1107 80-81 -0.18627638367260957 1107 82-83 0.22176261582877999 1107 84-85 0.24491751071934686 1107 86-87 0.13619939762094901 1107 88-89 0.32515705375652004 1107 90-91 -0.06134770652907662 1107 92-93 -3.6029340980078555 1107 94-95 -1.166661487295496 1107 96-97 -0.516007765575651 1107 98-99 -0.8690756554805006 1107 100-101 -0.5617438147096347 1108 1 0.34005542120610244 1108 2 0.38793598292282283 1108 3 0.3192124364770166 1108 4 -0.08650051380655555 1108 5 -0.29325261179373285 1108 6 5.897145017428329E-4 1108 7 0.23259544737891247 1108 8 0.5438318446967472 1108 9 0.5798692794415174 1108 10-11 0.2496883410024111 1108 12-13 0.22068638572057608 1108 14-15 0.18214587920719083 1108 16-17 0.23092717235400784 1108 18-19 0.3892679064072624 1108 20-21 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1.1371033994675948 2311 50-51 1.1633197556938732 2311 52-53 1.0259245843452014 2311 54-55 1.1657194583465902 2311 56-57 1.0112187023633616 2311 58-59 0.9893693647048174 2311 60-61 0.8498320137621285 2311 62-63 0.4166952512009239 2311 64-65 0.7898180508016637 2311 66-67 0.5651571891599723 2311 68-69 0.9712459140160163 2311 70-71 0.8675377216579747 2311 72-73 0.8062798212259175 2311 74-75 0.7852167929913207 2311 76-77 0.9794158062053491 2311 78-79 0.7852279270953524 2311 80-81 0.8604230433584519 2311 82-83 0.7897235000693854 2311 84-85 0.9392743017462095 2311 86-87 0.9852274328957051 2311 88-89 1.482800158606075 2311 90-91 1.9797782164831013 2311 92-93 2.7448343311432772 2311 94-95 1.994575327332715 2311 96-97 2.4070116447956345 2311 98-99 1.8290469384693395 2311 100-101 1.8288034993357698 2312 1 0.1228439586320782 2312 2 0.14521081102395783 2312 3 -0.03964312305307871 2312 4 -0.04175570767895209 2312 5 0.22998141481297552 2312 6 0.1796966920910137 2312 7 0.19100472282022452 2312 8 -0.7609188664620561 2312 9 0.06743464643353292 2312 10-11 0.07462403861939748 2312 12-13 0.13345658348234224 2312 14-15 -0.17474796082323962 2312 16-17 0.050277221467965205 2312 18-19 0.2394930357217575 2312 20-21 0.253066301033833 2312 22-23 0.12334766350698345 2312 24-25 0.3625463086840881 2312 26-27 0.07902685912002028 2312 28-29 0.12528538064067618 2312 30-31 0.31913820308177065 2312 32-33 0.33768749761032524 2312 34-35 0.2176437428105089 2312 36-37 0.24256765426326865 2312 38-39 0.3439793714511694 2312 40-41 0.12874397169583318 2312 42-43 0.07095224503432007 2312 44-45 -0.1266494926927848 2312 46-47 0.1098711624528903 2312 48-49 -0.17320439167495394 2312 50-51 -0.05426208199017779 2312 52-53 0.10083323932199661 2312 54-55 0.059968593651941404 2312 56-57 -0.19340131954797002 2312 58-59 -0.10480348244928805 2312 60-61 -0.02557452178069397 2312 62-63 0.0480399302457748 2312 64-65 0.16015400546470815 2312 66-67 0.18373507477363304 2312 68-69 -0.11792518367297333 2312 70-71 -0.13320651801301153 2312 72-73 -0.014173433639601996 2312 74-75 0.030540558758019642 2312 76-77 0.47002318608019067 2312 78-79 0.36484579802497663 2312 80-81 -0.05333262806331618 2312 82-83 0.5934249093926027 2312 84-85 0.4023777955140986 2312 86-87 -0.07457272656373704 2312 88-89 0.00892598243206777 2312 90-91 0.8665103877127187 2312 92-93 1.900264269367085 2312 94-95 1.1913098503023285 2312 96-97 0.736044448700671 2312 98-99 0.9684750067730477 2312 100-101 0.7856834860283612 2313 1 -0.2673184600277523 2313 2 -0.7528195792344121 2313 3 -0.32976367224098624 2313 4 -0.16957099352532623 2313 5 -0.6096846761484542 2313 6 -0.13836300679770375 2313 7 -1.1216904499785727 2313 8 -1.006587992960199 2313 9 -0.9254737117597998 2313 10-11 -1.786866514581007 2313 12-13 -0.37533600562112923 2313 14-15 -0.1478555479204715 2313 16-17 -1.1381833776143893 2313 18-19 -0.8930674569423545 2313 20-21 -1.459015658457453 2313 22-23 -0.6492487671205325 2313 24-25 -0.18941108912822813 2313 26-27 -0.7552877899614643 2313 28-29 -2.149653123572442 2313 30-31 -0.5058343200772768 2313 32-33 -0.23810060797053012 2313 34-35 -0.2465098832602095 2313 36-37 -0.4375321346448118 2313 38-39 -0.827585151149627 2313 40-41 -0.38607607680297207 2313 42-43 0.13624681421490692 2313 44-45 -0.44349236005378856 2313 46-47 -0.7526704889488656 2313 48-49 -0.33277505513163064 2313 50-51 -0.18260945904221515 2313 52-53 0.08095396296603852 2313 54-55 0.38655047463796777 2313 56-57 0.12739297922560056 2313 58-59 0.26361297923142146 2313 60-61 0.19055045859299113 2313 62-63 0.32931555349030717 2313 64-65 0.5423873928794407 2313 66-67 0.14115469411035164 2313 68-69 0.27305717881198177 2313 70-71 0.4350276370087087 2313 72-73 0.7565127023315092 2313 74-75 0.9952393602443834 2313 76-77 0.9521850464803912 2313 78-79 0.9995521011250652 2313 80-81 1.165300159222319 2313 82-83 1.1370738338705415 2313 84-85 1.1446363046119856 2313 86-87 1.4045884883487751 2313 88-89 1.4964567303255123 2313 90-91 1.9303700551993295 2313 92-93 2.6478436905216327 2313 94-95 1.7076563899649315 2313 96-97 1.1861718016777658 2313 98-99 1.3588860254224073 2313 100-101 1.5345538825671525 2314 1 -0.25344582088350975 2314 2 -0.478988168013732 2314 3 -1.278265910062963 2314 4 -0.5958992940806027 2314 5 -0.2700185851870245 2314 6 -2.9181093250698424 2314 7 -3.884481320407751 2314 8 -1.2845421965369965 2314 9 -1.7901736950457803 2314 10-11 -0.8980054823972239 2314 12-13 -1.5579738498835738 2314 14-15 -2.7886015504832855 2314 16-17 -1.6611651593292507 2314 18-19 -2.022345782568671 2314 20-21 -1.5137958690541495 2314 22-23 -2.9456487522636294 2314 24-25 -1.2924716124628617 2314 26-27 -1.9135603028219776 2314 28-29 -1.4221241990269675 2314 30-31 -1.6292163097888661 2314 32-33 -2.6354579352125285 2314 34-35 -0.7317969001785158 2314 36-37 -0.3760106014117639 2314 38-39 -0.38112109558478124 2314 40-41 -0.754578500336315 2314 42-43 -0.5153679461245773 2314 44-45 -1.1051169630957425 2314 46-47 -1.3843723479196512 2314 48-49 -2.2125223019606075 2314 50-51 -1.5518454424767647 2314 52-53 -0.9671313528361409 2314 54-55 -0.5505722991470137 2314 56-57 -1.017638313139802 2314 58-59 -0.8366108029662875 2314 60-61 -0.49918155990386026 2314 62-63 -0.6400234997531378 2314 64-65 -0.7938755372745128 2314 66-67 -0.6880744180036054 2314 68-69 -0.711278067348438 2314 70-71 -0.8261846867976317 2314 72-73 -0.5732317588269602 2314 74-75 -0.414718483275216 2314 76-77 -0.5672310769805229 2314 78-79 -0.014566605580974112 2314 80-81 -0.3549238086867561 2314 82-83 -0.4236816399720098 2314 84-85 -0.8095533438363951 2314 86-87 -0.45406658992856563 2314 88-89 -0.4036676962906398 2314 90-91 0.38611503634962574 2314 92-93 1.4865301540200662 2314 94-95 0.8965341361717769 2314 96-97 0.4308418854335905 2314 98-99 0.6217279664776214 2314 100-101 -0.01175324689224233 2315 1 -6.759154722335676 2315 2 -2.806280042528755 2315 3 -1.2980345979393988 2315 4 -0.6970891104350372 2315 5 -1.1485981957253983 2315 6 -1.4414807177461881 2315 7 -3.0324323975241114 2315 8 -2.5657194496131552 2315 9 -3.0004608029052164 2315 10-11 -2.0520811576734275 2315 12-13 -2.7244003362302465 2315 14-15 -4.253516748354919 2315 16-17 -3.7022914202759267 2315 18-19 -2.6611977206390947 2315 20-21 -3.7755185162107807 2315 22-23 -5.5358734132398695 2315 24-25 -3.353375798990598 2315 26-27 -4.340139026540712 2315 28-29 -2.9600265010629556 2315 30-31 -2.3762694820166317 2315 32-33 -1.5351734139103783 2315 34-35 -2.0299438166327164 2315 36-37 -2.699394926874575 2315 38-39 -2.319025245161697 2315 40-41 -3.329099063466785 2315 42-43 -7.7745040359889686 2315 44-45 -4.108026840049519 2315 46-47 -3.0858836363946907 2315 48-49 -5.91858777881092 2315 50-51 -6.734761231561492 2315 52-53 -6.392823256911818 2315 54-55 -4.341624658456773 2315 56-57 -3.7274358226546376 2315 58-59 -4.084098147310964 2315 60-61 -2.8092358776070014 2315 62-63 -2.8846286802578867 2315 64-65 -2.052817635341988 2315 66-67 -2.778160513410615 2315 68-69 -2.0426622450079712 2315 70-71 -1.6278696441194391 2315 72-73 -1.8102527838426639 2315 74-75 -2.459410498203347 2315 76-77 -2.246063800625354 2315 78-79 -1.278460113322172 2315 80-81 -0.8882626846428607 2315 82-83 -1.7264751218476349 2315 84-85 -1.8469106213000899 2315 86-87 -2.1377213953825063 2315 88-89 -1.13381586907267 2315 90-91 -0.27269958139415706 2315 92-93 0.5237673413237616 2315 94-95 0.5111925258551118 2315 96-97 0.011644087702372019 2315 98-99 0.3547952539180379 2315 100-101 0.5342689975004724 2316 1 -3.9513129106067595 2316 2 -2.504841625913727 2316 3 -4.573230361558352 2316 4 -4.247612085666475 2316 5 -4.8110021917444 2316 6 -4.78655047907419 2316 7 -3.0697448494047563 2316 8 -1.49812701350627 2316 9 -1.8718764977956468 2316 10-11 -3.0272140140383286 2316 12-13 -3.9799727922421155 2316 14-15 -3.0322850806554413 2316 16-17 -2.9594788087798634 2316 18-19 -3.056968850904063 2316 20-21 -5.988074616336597 2316 22-23 -6.765541225381909 2316 24-25 -4.778164073829572 2316 26-27 -4.098941347536709 2316 28-29 -4.769192181869677 2316 30-31 -6.731822220829471 2316 32-33 -4.6625746870780915 2316 34-35 -6.490434305431471 2316 36-37 -10.149660639604374 2316 38-39 -6.823611841222011 2316 40-41 -6.141278658982536 2316 42-43 -4.557614846647525 2316 44-45 -3.442416451902371 2316 46-47 -6.01350743223718 2316 48-49 -3.2327203159449773 2316 50-51 -4.570545131060111 2316 52-53 -5.0321909920628904 2316 54-55 -7.128969972335806 2316 56-57 -5.092818993555806 2316 58-59 -6.042072345947663 2316 60-61 -6.178745576591094 2316 62-63 -6.387159054133537 2316 64-65 -5.164182253297785 2316 66-67 -6.158636730712928 2316 68-69 -4.660481898360835 2316 70-71 -6.172327233363237 2316 72-73 -7.807102726568896 2316 74-75 -7.603008042003697 2316 76-77 -6.721206046132647 2316 78-79 -5.437549742075479 2316 80-81 -5.736820512370876 2316 82-83 -4.71200645341581 2316 84-85 -6.432225063678924 2316 86-87 -4.725204729654756 2316 88-89 -4.99318529426937 2316 90-91 -4.78572232189704 2316 92-93 -3.9703268881093194 2316 94-95 -3.879921589781569 2316 96-97 -4.893516184958324 2316 98-99 -5.8527564333108515 2316 100-101 -5.160058082664026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1874.0 3 643.0 4 689.0 5 861.0 6 1078.0 7 1526.0 8 2040.0 9 2543.0 10 3002.0 11 3720.0 12 4577.0 13 5532.0 14 6300.0 15 7441.0 16 8605.0 17 9560.0 18 8162.0 19 6923.0 20 7058.0 21 7673.0 22 8653.0 23 9836.0 24 11019.0 25 12507.0 26 14544.0 27 16427.0 28 19008.0 29 21711.0 30 24747.0 31 28616.0 32 32874.0 33 38531.0 34 45633.0 35 52989.0 36 57615.0 37 51003.0 38 15524.0 39 230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96529762272194 20.70105555938838 15.253323359925053 21.080323457964624 2 15.380551026411066 22.892071596393308 40.08232350559415 21.645053871601476 3 18.623517065323565 27.432158121597922 31.192012578844736 22.752312234233777 4 12.764709620604817 18.49295045801596 37.999073981606735 30.743265939772492 5 12.966069690763577 37.473491945909046 34.26420178076023 15.296236582567142 6 28.585355049995282 37.309295418733164 19.118746959255844 14.986602572015714 7 24.74291378897144 35.14873236163461 23.201485871151878 16.906867978242083 8 23.997966612503518 33.70448706892639 22.470202703316055 19.827343615254037 9 25.664630958259504 17.819407732303134 23.005925581665554 33.51003572777181 10-11 23.058348493266084 27.823741725581613 28.817216600035582 20.300693181116717 12-13 24.967728582399687 26.266936820643537 29.179625161357087 19.585709435599686 14-15 21.95218931817171 27.03964312202863 29.241625232739594 21.76654232706007 16-17 19.79068038407036 30.140735623460717 29.461159491597893 20.60742450087103 18-19 19.25648134530923 27.976135358850197 33.68483930766396 19.082543988176617 20-21 20.187314338008324 27.37565639911972 33.63005962972916 18.8069696331428 22-23 20.016960910005483 27.482212835813307 33.35697086137452 19.143855392806685 24-25 19.791581528402126 27.403078599645823 33.64555237706035 19.159787494891702 26-27 19.487166912330316 27.810124248591634 33.53113571077623 19.171573128301812 28-29 19.29587953754916 28.03585537966924 33.51560726189503 19.152657820886574 30-31 19.51525422497348 27.968561547292094 33.28020329279103 19.2359809349434 32-33 19.352287020765505 28.083062014975862 33.03944673759542 19.525204226663213 34-35 19.78630022040174 28.040785672706605 32.902428414842014 19.270485692049647 36-37 19.735179929617434 27.91345215120899 32.702735838347145 19.648632080826427 38-39 19.42488769182291 28.120042389696437 33.00938693856128 19.44568297991938 40-41 19.644605150128854 27.97410951515273 32.892774963813984 19.48851037090444 42-43 19.538980526762646 28.249678775578772 32.388865430803946 19.82247526685463 44-45 19.50736509879302 28.40604607284016 32.199596311049326 19.88699251731749 46-47 19.72263567848551 28.374491781781064 31.78628544132458 20.116587098408846 48-49 19.80811692823595 28.561748909837846 31.418287112516722 20.21184704940948 50-51 19.59979434708834 29.018679533901846 31.180578516900965 20.20094760210885 52-53 19.78528206483871 29.198223462540184 30.829047786459395 20.187446686161703 54-55 19.404693448739156 28.911320835275085 31.01631433552172 20.66767138046404 56-57 19.57357037187722 29.295559250705853 30.633260166935628 20.49761021048129 58-59 19.666804695899806 28.825770784296633 30.7797925202685 20.72763199953506 60-61 19.763615362822087 28.74990011405159 30.96682333626332 20.519661186863 62-63 19.677232476881613 28.659169990048017 30.914075155635945 20.749522377434424 64-65 19.43016471673479 28.76523280243616 30.822330729994206 20.98227175083484 66-67 19.509444447471218 28.90068120608527 30.409831450981674 21.180042895461835 68-69 19.480326085574763 28.649893853297815 30.657510265085268 21.212269796042154 70-71 19.501229536481095 28.75063111362951 30.43202679190867 21.316112557980727 72-73 19.68742960535399 28.57695633533648 30.230730941671453 21.504883117638084 74-75 19.61231760958868 28.753685962365484 30.07091219279105 21.563084235254788 76-77 19.592862373019322 28.521346199618808 30.29803099318833 21.587760434173543 78-79 19.597958907432258 28.55194893089737 30.105116085550016 21.744976076120363 80-81 19.629278343771976 28.561902321938952 30.077458531613 21.731360802676072 82-83 19.764792455915618 28.361814432240422 29.98410145994022 21.889291651903736 84-85 19.77263809565331 28.13894217494296 29.791294269811512 22.297125459592216 86-87 19.8444272473941 27.879839849441034 30.008519806278862 22.267213096886003 88-89 20.076582755707367 27.78754871589887 29.693850668168647 22.442017860225118 90-91 19.82388228265584 27.758727456741223 29.94537695538588 22.472013305217057 92-93 19.79223848803894 27.588584059337506 30.181864272935016 22.43731317968854 94-95 20.241908669211607 27.214725286305836 29.651527236982915 22.89183880749964 96-97 20.251823530908258 27.076662802150075 29.506765326927198 23.164748340014466 98-99 20.300541432588098 27.004018487587402 29.24690162066277 23.448538459161732 100-101 20.61132942429541 26.843676046661262 29.42156901948097 23.12342550956236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4802.0 1 3485.5 2 2191.5 3 2073.5 4 1744.0 5 1649.5 6 2166.5 7 3274.0 8 4533.5 9 4601.5 10 3527.0 11 2642.5 12 2260.5 13 2152.5 14 2069.0 15 2058.0 16 2064.0 17 2014.0 18 2032.0 19 2160.5 20 2290.0 21 2470.5 22 2725.5 23 2973.0 24 3214.5 25 3567.5 26 4113.5 27 4808.5 28 5445.0 29 6043.0 30 6721.5 31 7509.5 32 8553.0 33 9682.5 34 10866.0 35 11997.0 36 13033.5 37 14282.0 38 14963.5 39 15019.0 40 15260.5 41 15966.5 42 16716.0 43 17343.5 44 17967.0 45 18224.0 46 18299.0 47 18601.5 48 19857.5 49 20398.0 50 19175.0 51 17928.0 52 16769.0 53 16003.0 54 15130.5 55 13622.5 56 12266.0 57 10850.5 58 9570.0 59 8509.0 60 7416.5 61 6013.0 62 4672.5 63 3588.5 64 2629.0 65 1977.0 66 1436.5 67 1048.0 68 759.0 69 495.0 70 342.5 71 267.0 72 211.0 73 161.5 74 125.5 75 93.5 76 64.0 77 48.5 78 30.0 79 15.5 80 11.0 81 8.5 82 8.0 83 6.5 84 2.5 85 2.5 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08634544709164589 2 0.09432695900768039 3 0.09215018303058008 4 0.09595954099050563 5 0.09033620304966315 6 0.07654995519469447 7 0.08815942707256283 8 0.08507566110500404 9 0.08017791515652833 10-11 0.08707103908401266 12-13 0.08652684508973758 14-15 0.09024550405061729 16-17 0.08879432006588375 18-19 0.09088039704393822 20-21 0.09831771496569765 22-23 0.10811320686264907 24-25 0.12679720066609343 26-27 0.13142284961743161 28-29 0.13223914060884423 30-31 0.13387172259166946 32-33 0.13087865562315656 34-35 0.12489252168613069 36-37 0.1290646756422396 38-39 0.12126456172429681 40-41 0.11736450476532541 42-43 0.11727380576627955 44-45 0.10929229385024508 46-47 0.12670650166704758 48-49 0.14194393350674983 50-51 0.1509231344122886 52-53 0.15917674332546067 54-55 0.15754416134263544 56-57 0.12743209365941438 58-59 0.12081106672906756 60-61 0.11718310676723373 62-63 0.11428073879776662 64-65 0.10357825691035673 66-67 0.1148249327920417 68-69 0.11264815681494139 70-71 0.11972267874051742 72-73 0.1462067864619046 74-75 0.15872324833023144 76-77 0.16235120829206529 78-79 0.16008373331591913 80-81 0.16597916825389916 82-83 0.16489078026534898 84-85 0.14257882650007075 86-87 0.1436672144886209 88-89 0.16126282030351513 90-91 0.25658746830069984 92-93 0.5356682883647697 94-95 0.16343959628061544 96-97 0.1755932621527589 98-99 0.1935516639638365 100-101 0.19881220590849558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 551274.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.69867921847201 #Duplication Level Percentage of deduplicated Percentage of total 1 87.99126999304143 61.32875301270957 2 7.5256212955542825 10.490517291970795 3 1.8776052338377518 3.9259981467654477 4 0.7728152904365285 2.1545682009306355 5 0.42790388741786023 1.49121678927373 6 0.26710969968702797 1.1170315964777136 7 0.18536979014475263 0.9044020678066212 8 0.14511511318547 0.8091465378933065 9 0.11150365280991673 0.6994491595997636 >10 0.6392736895315886 8.080223037797651 >50 0.03760824015876759 1.8084766631989238 >100 0.015931263414963218 1.8066559150279569 >500 0.0010446730108168312 0.589051742169162 >1k 0.0015670095162252473 3.4771527746957145 >5k 2.611682527042078E-4 1.3173570636830114 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7237 1.3127773121895827 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4346 0.7883556997064981 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4241 0.7693089099068703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3930 0.7128941325003537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3681 0.6677260309755222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.27100860914898944 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1410 0.2557711773092872 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 0.16616056625199085 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 892 0.1618070142977902 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.15690926834931448 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 0.10212707292562319 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.8139799809169306E-4 0.0 4 0.0 0.0 0.0 1.8139799809169306E-4 0.0 5 0.0 0.0 0.0 1.8139799809169306E-4 0.0 6 0.0 0.0 0.0 1.8139799809169306E-4 0.0 7 0.0 0.0 0.0 1.8139799809169306E-4 0.0 8 0.0 0.0 0.0 1.8139799809169306E-4 0.0 9 0.0 0.0 0.0 1.8139799809169306E-4 0.0 10-11 0.0 0.0 0.0 1.8139799809169306E-4 0.0 12-13 0.0 0.0 0.0 2.720969971375396E-4 0.0 14-15 0.0 0.0 0.0 8.162909914126188E-4 0.0 16-17 0.0 0.0 0.0 0.0011790869875960047 0.0 18-19 0.0 0.0 0.0 0.0017232809818710842 0.0 20-21 0.0 0.0 0.0 0.0021767759771003166 0.0 22-23 0.0 0.0 0.0 0.0037186589608797075 0.0 24-25 9.069899904584653E-5 0.0 0.0 0.006439628932255104 0.0 26-27 1.8139799809169306E-4 0.0 0.0 0.011155976882639123 0.0 28-29 1.8139799809169306E-4 0.0 0.0 0.024851525738561947 0.0 30-31 1.8139799809169306E-4 0.0 0.0 0.06475908531873442 0.0 32-33 1.8139799809169306E-4 0.0 0.0 0.16090002430733175 0.0 34-35 1.8139799809169306E-4 0.0 0.0 0.29032749594575474 0.0 36-37 3.627959961833861E-4 0.0 0.0 0.47226968803172287 0.0 38-39 5.441939942750792E-4 0.0 0.0 0.7048219215852733 0.0 40-41 5.441939942750792E-4 0.0 0.0 0.9552418579508557 0.0 42-43 7.255919923667722E-4 0.0 0.0 1.2020338343546042 0.0 44-45 7.255919923667722E-4 0.0 0.0 1.5053312871639148 0.0 46-47 7.255919923667722E-4 0.0 0.0 1.8194219208596814 0.0 48-49 7.255919923667722E-4 0.0 0.0 2.1375033105134653 0.0 50-51 7.255919923667722E-4 0.0 0.0 2.478350148927756 0.0 52-53 7.255919923667722E-4 0.0 0.0 2.809582893443188 0.0 54-55 7.255919923667722E-4 0.0 0.0 3.1508832268527085 0.0 56-57 9.069899904584653E-4 0.0 0.0 3.556489150585734 0.0 58-59 9.069899904584653E-4 0.0 0.0 3.9930234329933936 0.0 60-61 9.069899904584653E-4 0.0 0.0 4.456767415114807 0.0 62-63 9.069899904584653E-4 0.0 0.0 4.923141668208549 0.0 64-65 9.069899904584653E-4 0.0 0.0 5.423800142941623 0.0 66-67 9.069899904584653E-4 0.0 0.0 5.988492111001063 0.0 68-69 9.069899904584653E-4 0.0 0.0 6.615131495408816 0.0 70-71 0.0012697859866418514 0.0 0.0 7.302720607175379 0.0 72-73 0.0014511839847335445 0.0 0.0 7.9892213309533915 0.0 74-75 0.0014511839847335445 0.0 0.0 8.718350584282952 0.0 76-77 0.0014511839847335445 1.8139799809169306E-4 0.0 9.474598838327221 0.0 78-79 0.0014511839847335445 1.8139799809169306E-4 0.0 10.294971284696903 0.0 80-81 0.0016325819828252376 1.8139799809169306E-4 0.0 11.170851518482642 0.0 82-83 0.0016325819828252376 1.8139799809169306E-4 0.0 11.994307730819882 0.0 84-85 0.0017232809818710842 1.8139799809169306E-4 0.0 12.809238237246813 0.0 86-87 0.0018139799809169306 2.720969971375396E-4 0.0 13.701988484855082 0.0 88-89 0.0018139799809169306 3.627959961833861E-4 0.0 14.61632509423626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4435 0.0 39.903763 1 GGTATCA 2480 0.0 39.219395 1 TATCAAC 5470 0.0 32.266678 2 ATCAACG 5695 0.0 31.408434 3 TCAACGC 5695 0.0 31.241812 4 CAACGCA 5760 0.0 30.889256 5 AACGCAG 5925 0.0 30.026321 6 GGGTCGC 35 0.008389733 27.143974 94-95 ACGCAGA 6550 0.0 26.509346 7 CGCAGAG 6585 0.0 25.936176 8 CTACACT 210 1.2787496E-9 24.852613 4 GCAGAGT 6475 0.0 24.549526 9 GTACATG 2155 0.0 23.337656 1 GTATAGA 125 2.2712903E-4 22.77403 1 AGTACTT 4340 0.0 22.743176 12-13 GAGTACT 4190 0.0 22.538063 12-13 TACATGG 2150 0.0 22.509214 2 CATATAG 130 2.96155E-4 21.898106 3 GTATCAC 110 0.0028945622 21.566317 1 AGAGTAC 5625 0.0 21.553915 10-11 >>END_MODULE