FastQCFastQC Report
Fri 27 May 2016
SRR939699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551274
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69041.252371778825049No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51690.9376462521359614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.7800113917942801No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.7798299937961884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42360.7684019199164118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.288422816965792No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.21459383174247287No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.18448176405925185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.17123971019855827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.14348581649052922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42750.037.752541
GGTATCA25250.037.4561231
TAGCCCG501.1842367E-633.64621460-61
TTAGCGG403.8916347E-430.42521564-65
TATCAAC53500.030.0733382
ATCAACG54200.029.6795433
TCAACGC55050.029.1358364
CAACGCA57000.028.1390825
AACGCAG58450.027.4111546
GGGTAGC950.00130618324.7288486
CGCAGAG65250.023.9806278
ACGCAGA66100.023.8833527
GCAGAGT66550.022.947339
GTACATG21650.022.8202081
TACATGG20950.022.4556312
AGTACTT43450.021.89939312-13
GCTTAGC1605.4160006E-520.5577188
ACATGGG21250.020.3638233
GAGTACT42400.020.16983612-13
AGAGTAC57500.020.0214310-11
Produced by FastQC (version 0.11.5)