##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939699_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 551274 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.343959628061544 31.0 31.0 34.0 30.0 34.0 2 31.541240834866148 31.0 31.0 34.0 30.0 34.0 3 31.567487311210034 31.0 31.0 34.0 30.0 34.0 4 35.416847157674766 37.0 35.0 37.0 33.0 37.0 5 35.1188120607901 37.0 35.0 37.0 32.0 37.0 6 35.15398694660006 37.0 35.0 37.0 32.0 37.0 7 35.08116834822611 37.0 35.0 37.0 32.0 37.0 8 35.04383301225888 37.0 35.0 37.0 32.0 37.0 9 36.59478408196287 39.0 35.0 39.0 32.0 39.0 10-11 36.496342109368484 38.0 35.0 39.0 32.0 39.0 12-13 36.422290004607504 38.0 35.0 39.0 32.0 39.0 14-15 37.36708333786828 39.0 36.0 41.0 32.0 41.0 16-17 37.34732818888611 39.0 36.0 40.5 32.0 41.0 18-19 37.33295693248729 39.0 36.0 40.5 32.0 41.0 20-21 37.28701154054064 39.0 36.0 41.0 31.5 41.0 22-23 37.115875227200995 39.0 36.0 40.0 31.0 41.0 24-25 36.95767349811527 39.0 35.5 40.0 31.0 41.0 26-27 36.63545895507497 38.5 35.0 40.0 30.5 41.0 28-29 36.36246313085689 38.0 35.0 40.0 30.0 41.0 30-31 36.00111650467825 38.0 35.0 40.0 30.0 41.0 32-33 35.6754944002438 38.0 34.0 40.0 29.0 41.0 34-35 35.51711762208992 38.0 34.0 40.0 28.5 41.0 36-37 35.285758080373824 38.0 34.0 40.0 27.0 41.0 38-39 35.202468282560034 38.0 34.0 40.0 27.0 41.0 40-41 35.019020487089904 38.0 34.0 40.0 26.0 41.0 42-43 34.71787441453796 38.0 33.0 40.0 25.0 41.0 44-45 34.37727337041109 38.0 33.0 40.0 24.0 41.0 46-47 33.9167963662353 38.0 33.0 40.0 23.0 41.0 48-49 33.48663096754065 37.0 33.0 40.0 20.5 41.0 50-51 33.18351037777947 37.0 33.0 40.0 19.5 41.0 52-53 32.91850150741736 37.0 32.0 40.0 17.5 41.0 54-55 32.58168986747062 36.5 32.0 40.0 13.5 41.0 56-57 30.56243265599321 35.5 27.5 39.0 5.0 40.5 58-59 25.713630608372604 31.5 12.0 37.5 2.0 40.0 60-61 26.384612733413874 31.5 17.5 37.0 2.0 40.0 62-63 29.265355340538463 34.0 27.0 38.0 2.0 40.0 64-65 29.421619739004562 34.5 28.0 38.0 2.0 40.0 66-67 23.0716540232262 26.0 15.0 34.5 2.0 38.0 68-69 16.048081353374183 13.0 6.5 28.0 2.0 34.5 70-71 14.987329349833296 12.0 6.0 24.5 2.0 32.5 72-73 16.00158360452334 12.5 6.0 27.5 2.0 34.0 74-75 16.88664076303254 15.5 6.5 28.5 2.0 34.0 76-77 18.00757518040031 19.0 7.0 29.0 2.0 33.5 78-79 20.81883147037589 25.0 7.0 33.0 2.0 35.0 80-81 24.102134328845548 30.5 14.0 34.0 2.0 36.0 82-83 25.243641093176898 32.0 18.0 35.0 2.0 36.0 84-85 25.250599520383695 33.0 18.0 35.0 2.0 36.0 86-87 24.976701241125102 33.0 11.5 35.0 2.0 35.0 88-89 24.49189241647529 32.0 4.0 35.0 2.0 35.0 90-91 23.15913048683595 31.5 2.0 34.5 2.0 35.0 92-93 20.754555810722074 30.0 2.0 34.0 2.0 35.0 94-95 14.12200103759655 2.0 2.0 31.0 2.0 34.0 96-97 11.849197495256442 2.0 2.0 27.0 2.0 34.0 98-99 11.519852196911156 2.0 2.0 28.0 2.0 34.0 100-101 11.275030202766683 2.0 2.0 28.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3444165932570904 1101 2 0.05076372488688108 1101 3 0.3099764313260849 1101 4 0.2549708003571496 1101 5 0.1477656801725047 1101 6 -0.004445371669341114 1101 7 -0.19848961728416015 1101 8 0.08196083793276188 1101 9 0.19093612391507975 1101 10-11 0.29673400276517725 1101 12-13 0.14659651518139327 1101 14-15 0.30140809079154707 1101 16-17 0.3478089209264823 1101 18-19 0.4020263266643198 1101 20-21 0.17890710797848186 1101 22-23 0.3130580150453426 1101 24-25 0.27639821492822847 1101 26-27 0.5333796847609662 1101 28-29 0.34241473217164753 1101 30-31 0.43098098626838777 1101 32-33 0.4534845164941643 1101 34-35 0.3749931392441326 1101 36-37 0.26857331201117063 1101 38-39 0.5270517029279489 1101 40-41 0.7890004867722809 1101 42-43 0.5002608284011387 1101 44-45 0.301811518237848 1101 46-47 0.510295316120029 1101 48-49 0.35375416223593703 1101 50-51 0.49882116921317277 1101 52-53 0.07801750416733455 1101 54-55 -0.11132972928064788 1101 56-57 -1.559241642341913 1101 58-59 -8.745421215462923 1101 60-61 -9.063362410498826 1101 62-63 -3.7191056268716167 1101 64-65 -5.016085422357509 1101 66-67 -9.252824749901277 1101 68-69 -4.035993325435724 1101 70-71 -4.104402860989373 1101 72-73 -4.144382212573458 1101 74-75 -6.121878340673618 1101 76-77 -7.086419943650718 1101 78-79 -10.222117032259273 1101 80-81 -7.733232448716564 1101 82-83 -4.633836639403643 1101 84-85 -3.932217091997938 1101 86-87 -3.775301230531941 1101 88-89 -3.952672118531229 1101 90-91 -9.268098629084589 1101 92-93 -15.192700348863633 1101 94-95 -10.045763420613397 1101 96-97 -8.193183694625809 1101 98-99 -8.290702029805722 1101 100-101 -8.030155660425212 1102 1 0.3786262002439891 1102 2 0.4635934192100244 1102 3 0.6393301431164815 1102 4 0.37356468682003197 1102 5 0.43428969763975545 1102 6 0.37544982483721157 1102 7 0.5984972822791548 1102 8 0.5831224099851653 1102 9 0.6909099230417155 1102 10-11 0.6638737407564435 1102 12-13 0.7693519736966792 1102 14-15 0.916643898651806 1102 16-17 0.8117434187430774 1102 18-19 0.6574761083237135 1102 20-21 0.7203743568867864 1102 22-23 0.7766431678837691 1102 24-25 0.7333152454959091 1102 26-27 0.9163491170753773 1102 28-29 0.8727990116476292 1102 30-31 0.6881250910718748 1102 32-33 0.8442880099439449 1102 34-35 0.8721547112965382 1102 36-37 0.8427043163779899 1102 38-39 1.0048770304388697 1102 40-41 0.8872319278203165 1102 42-43 1.0047455445583466 1102 44-45 1.2125145750064164 1102 46-47 1.5499896392641261 1102 48-49 1.3478764329782962 1102 50-51 1.5706071954140413 1102 52-53 1.492017504167336 1102 54-55 1.956246689933323 1102 56-57 -0.9243988475820863 1102 58-59 -9.242229075724929 1102 60-61 -9.087938829712797 1102 62-63 -2.9797038801467295 1102 64-65 -3.9479806188640616 1102 66-67 -6.261278898372893 1102 68-69 -4.423840487007775 1102 70-71 -3.8638963107710307 1102 72-73 -3.4259979330974755 1102 74-75 -4.560930742420343 1102 76-77 -6.510092432733687 1102 78-79 -8.608876857586782 1102 80-81 -4.795079610288614 1102 82-83 -2.4456619669145603 1102 84-85 -2.1543000614302557 1102 86-87 -2.207213894287399 1102 88-89 -2.307663384906771 1102 90-91 -8.104871554848781 1102 92-93 -12.228591178557956 1102 94-95 -9.516540713189816 1102 96-97 -8.266406402049391 1102 98-99 -8.239994606224936 1102 100-101 -7.853374001036565 1103 1 0.07476782373276336 1103 2 0.05644661437410292 1103 3 0.07936123119937832 1103 4 0.062065550377887746 1103 5 0.0651808893495982 1103 6 0.0604308265643283 1103 7 0.08120885395445754 1103 8 0.01893242725631694 1103 9 0.20801873133186888 1103 10-11 0.3558858305664643 1103 12-13 0.12857304450842122 1103 14-15 0.12218966721830071 1103 16-17 0.1560594809192395 1103 18-19 0.17483707550851335 1103 20-21 0.2084641669040579 1103 22-23 0.3115119070892973 1103 24-25 0.17893873426965712 1103 26-27 0.053741172343080734 1103 28-29 -0.10027180009674908 1103 30-31 0.11466049694407587 1103 32-33 0.09757989250007171 1103 34-35 0.21999581665058798 1103 36-37 -0.039637652533933476 1103 38-39 0.11157478518843078 1103 40-41 0.28965852540235204 1103 42-43 0.13544157219219954 1103 44-45 0.29377364236392367 1103 46-47 0.374100174670005 1103 48-49 0.36902669204565797 1103 50-51 0.156895623738734 1103 52-53 0.21532320364919855 1103 54-55 0.00810333932883367 1103 56-57 -2.4165905574266446 1103 58-59 -9.379329248436502 1103 60-61 -10.107665945429552 1103 62-63 -4.707291099490426 1103 64-65 -5.897023796756979 1103 66-67 -6.524947292155275 1103 68-69 -4.601099554365289 1103 70-71 -4.395806500753759 1103 72-73 -4.20883040287295 1103 74-75 -5.727609498897026 1103 76-77 -6.825859272111925 1103 78-79 -9.018603973303536 1103 80-81 -5.69341639785338 1103 82-83 -3.66352379765722 1103 84-85 -3.2542016158344005 1103 86-87 -3.049428056636277 1103 88-89 -3.1582799652176554 1103 90-91 -5.831519223242562 1103 92-93 -9.630889969576955 1103 94-95 -7.724644340132821 1103 96-97 -7.574320046263552 1103 98-99 -7.507977335067768 1103 100-101 -7.468975037305995 1104 1 0.018532240512445952 1104 2 0.1647477816261329 1104 3 0.08072611627084214 1104 4 0.1302895190347968 1104 5 0.11325614059277456 1104 6 -0.027744806035272518 1104 7 0.09977245006439261 1104 8 0.12878683951536374 1104 9 -0.02965383534754551 1104 10-11 0.29759857297121073 1104 12-13 0.3497815038980221 1104 14-15 0.35886537516187644 1104 16-17 0.252709861696097 1104 18-19 0.21930161167941975 1104 20-21 0.1284213367525595 1104 22-23 0.036267328957592326 1104 24-25 0.08513403744222359 1104 26-27 -0.12861732587763441 1104 28-29 0.031033910976489665 1104 30-31 0.2776150239577859 1104 32-33 0.3401672045748185 1104 34-35 0.40084598646431857 1104 36-37 0.3950130412101984 1104 38-39 0.2969441445328229 1104 40-41 -0.029513038622638987 1104 42-43 0.1742958801288168 1104 44-45 0.01264209178493303 1104 46-47 0.2963654781903102 1104 48-49 0.20727911754205053 1104 50-51 0.1593051819912219 1104 52-53 0.2801987122210221 1104 54-55 0.30289098523534363 1104 56-57 -3.170533075770006 1104 58-59 -9.680893505255131 1104 60-61 -10.114328091457764 1104 62-63 -4.7489186452474 1104 64-65 -5.906376592018422 1104 66-67 -6.461171515822556 1104 68-69 -4.54228344002791 1104 70-71 -4.369305706744186 1104 72-73 -4.772877127728346 1104 74-75 -5.311380406849871 1104 76-77 -6.646998473002142 1104 78-79 -8.926272830741146 1104 80-81 -5.433314509617471 1104 82-83 -3.398695523961539 1104 84-85 -3.019454423846362 1104 86-87 -2.9991736258310233 1104 88-89 -2.9045895594034192 1104 90-91 -4.862750749479655 1104 92-93 -6.515745540974063 1104 94-95 -7.47863467292136 1104 96-97 -7.3651782141299265 1104 98-99 -7.433766418305472 1104 100-101 -7.500676014459384 1105 1 0.3129680805858719 1105 2 0.29287547049207774 1105 3 0.35295407474040985 1105 4 0.3322484475037939 1105 5 0.16572559507564932 1105 6 0.2052959786833668 1105 7 0.02815540193727628 1105 8 -0.03668955582680411 1105 9 0.21345693158872336 1105 10-11 0.38460023648293884 1105 12-13 0.43642034976505073 1105 14-15 0.33077210378001354 1105 16-17 0.40795709395675317 1105 18-19 0.2617154245630289 1105 20-21 0.22885298936541432 1105 22-23 0.39328419352479216 1105 24-25 0.4979990061796755 1105 26-27 0.5723320230582871 1105 28-29 0.36096995181857494 1105 30-31 0.22549261243940322 1105 32-33 0.3664076680636086 1105 34-35 0.5225820617238881 1105 36-37 0.3961402138138794 1105 38-39 0.5031163466781834 1105 40-41 0.1993259449143352 1105 42-43 -0.07406642125361884 1105 44-45 0.08710431859615397 1105 46-47 0.36434861362310755 1105 48-49 0.10221831332017217 1105 50-51 0.3902909560978003 1105 52-53 0.4361371622869967 1105 54-55 0.1047509634376027 1105 56-57 1.0233191011358649 1105 58-59 -8.105160699656555 1105 60-61 -9.223887547661516 1105 62-63 -3.4590115724544184 1105 64-65 -5.330100276983718 1105 66-67 -6.402065223159216 1105 68-69 -4.018361854529143 1105 70-71 -3.695075797950519 1105 72-73 -1.917279984379526 1105 74-75 -4.19222134071094 1105 76-77 -6.379895851537107 1105 78-79 -8.751064891774817 1105 80-81 -5.003207815416822 1105 82-83 -2.464174787427382 1105 84-85 -1.7029239930541884 1105 86-87 -1.5582053472788537 1105 88-89 -1.9577574020007908 1105 90-91 -4.317410016387242 1105 92-93 -6.785112546079324 1105 94-95 -7.39787404652315 1105 96-97 -7.112440590083578 1105 98-99 -7.29235358058391 1105 100-101 -7.291485112147677 1106 1 0.24148228880487466 1106 2 0.3198222014978498 1106 3 0.24420837189876465 1106 4 0.2652916240894072 1106 5 0.2741937566803401 1106 6 0.47322104254939035 1106 7 0.3568367656739895 1106 8 0.30181613692243303 1106 9 0.534061214554626 1106 10-11 0.44920584407748265 1106 12-13 0.3223445936228728 1106 14-15 0.4374520167329905 1106 16-17 0.36982828959178704 1106 18-19 0.3539631931945664 1106 20-21 0.5174075672188891 1106 22-23 0.6351708431605232 1106 24-25 0.6231086034294933 1106 26-27 0.39125686615287236 1106 28-29 0.48703517400925733 1106 30-31 0.6775568253892246 1106 32-33 0.5527529545933945 1106 34-35 0.7664351541009609 1106 36-37 0.8287781171159949 1106 38-39 1.195548617154735 1106 40-41 1.1787263927649647 1106 42-43 0.8304909320122178 1106 44-45 0.9221123609842792 1106 46-47 0.6351925912936522 1106 48-49 0.8687399016129831 1106 50-51 0.928315682498912 1106 52-53 0.909198315975452 1106 54-55 1.1111359888263124 1106 56-57 2.274416650572899 1106 58-59 -7.238609149525669 1106 60-61 -8.998927759602097 1106 62-63 -2.9203939170470967 1106 64-65 -4.181943718495059 1106 66-67 -6.7573526991109 1106 68-69 -4.6565600442033475 1106 70-71 -3.7104793366012903 1106 72-73 -4.013599409112235 1106 74-75 -4.071237015483073 1106 76-77 -5.985262174431103 1106 78-79 -8.837910067918887 1106 80-81 -4.601954149034004 1106 82-83 -2.213772668021573 1106 84-85 -1.5808941573712154 1106 86-87 -1.502077362922087 1106 88-89 -1.2349438277112021 1106 90-91 -3.666203658169813 1106 92-93 -5.3751557542037105 1106 94-95 -6.171736285145536 1106 96-97 -6.343963597621345 1106 98-99 -5.679618222461098 1106 100-101 -6.085909056387119 1107 1 0.23194708309628354 1107 2 0.1580620100419452 1107 3 0.23117734175824367 1107 4 0.24219287018166114 1107 5 0.1789857927161549 1107 6 0.24194218476930018 1107 7 0.2208911702248244 1107 8 0.25000526227718467 1107 9 0.510173080936454 1107 10-11 0.17817764568004435 1107 12-13 0.08581886673572825 1107 14-15 0.05433659814246994 1107 16-17 0.2473444374187963 1107 18-19 0.2235745803101281 1107 20-21 0.21844566418729272 1107 22-23 0.42621702187358323 1107 24-25 0.3700826478728274 1107 26-27 0.30268188447775657 1107 28-29 0.20059527650332143 1107 30-31 0.2477583678121178 1107 32-33 0.2819722204702586 1107 34-35 0.13089834457327498 1107 36-37 0.4177195965138054 1107 38-39 0.3870468097257955 1107 40-41 0.17068693630928067 1107 42-43 0.38851973810673 1107 44-45 0.3345162727992843 1107 46-47 0.44313055607227625 1107 48-49 0.13987303739255452 1107 50-51 0.17001466570266643 1107 52-53 0.11876453302981815 1107 54-55 -0.6417584034452801 1107 56-57 0.8379288264416864 1107 58-59 -8.580549958577219 1107 60-61 -10.466719814432661 1107 62-63 -4.232068905613623 1107 64-65 -5.006955151971024 1107 66-67 -6.7609800868278995 1107 68-69 -3.7615365820841777 1107 70-71 -4.074280011959486 1107 72-73 -2.5544529415864403 1107 74-75 -4.232979978413551 1107 76-77 -6.559055081290563 1107 78-79 -7.084788572357581 1107 80-81 -2.5254140754838623 1107 82-83 -1.4509624762524211 1107 84-85 -1.139041996914127 1107 86-87 -1.0189660165284877 1107 88-89 -0.7335326548558392 1107 90-91 -0.6511177348148252 1107 92-93 -2.946453657335546 1107 94-95 -5.479141731865537 1107 96-97 -6.200807081166538 1107 98-99 -6.032765404187584 1107 100-101 -5.955586225145224 1108 1 -0.11948172555702286 1108 2 -0.01203221316771419 1108 3 -0.2009295533422062 1108 4 0.14154782341362449 1108 5 0.08751398094498342 1108 6 -0.010270242616414293 1108 7 -0.03248079529251413 1108 8 -0.27155212627116043 1108 9 -0.028479621646312125 1108 10-11 -0.11230669769211943 1108 12-13 -0.008787992576507975 1108 14-15 -0.10116891753705914 1108 16-17 -0.2955871040225233 1108 18-19 -0.2710500299562213 1108 20-21 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32-33 -0.5997265620050527 2316 34-35 -0.5715611356979977 2316 36-37 -0.722971457756806 2316 38-39 -0.6912650460638687 2316 40-41 -0.34472617782630977 2316 42-43 -0.27838195999539295 2316 44-45 -0.48034699147810755 2316 46-47 -0.17868067038978097 2316 48-49 -0.13021828469019425 2316 50-51 -0.12030172990472465 2316 52-53 -0.5758987071259298 2316 54-55 -0.2563671534182177 2316 56-57 1.327970915623741 2316 58-59 6.114399339575616 2316 60-61 4.9350447768445775 2316 62-63 1.8515110561009962 2316 64-65 1.3201869585494173 2316 66-67 7.669169189058799 2316 68-69 14.228576635032296 2316 70-71 14.608497131851918 2316 72-73 13.19866509058194 2316 74-75 12.119366161040496 2316 76-77 10.42196767655593 2316 78-79 7.735400009444181 2316 80-81 4.47077284872778 2316 82-83 2.8481413117739613 2316 84-85 2.5017418329231162 2316 86-87 2.1922360146379205 2316 88-89 2.2815588373154476 2316 90-91 2.7718151482477573 2316 92-93 4.984048165704337 2316 94-95 11.175532146555176 2316 96-97 12.166121831101796 2316 98-99 11.869619237126614 2316 100-101 10.816711609163798 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 19.0 5 77.0 6 207.0 7 442.0 8 665.0 9 1240.0 10 1845.0 11 2684.0 12 3559.0 13 4421.0 14 5544.0 15 7226.0 16 9384.0 17 11973.0 18 12588.0 19 11675.0 20 11600.0 21 11830.0 22 11620.0 23 10740.0 24 10525.0 25 11984.0 26 15776.0 27 22875.0 28 31080.0 29 40188.0 30 47344.0 31 49339.0 32 47613.0 33 42943.0 34 37421.0 35 32016.0 36 23874.0 37 14153.0 38 4578.0 39 222.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.815467464038434 21.984635594327433 16.007613255337986 21.19228368629614 2 15.705632323408238 23.91764584229059 38.697165787721616 21.679556046579552 3 18.574859597440103 27.545567342437128 31.602185491009884 22.27738756911289 4 13.104191382143906 19.468902941187142 37.246088152171154 30.1808175244978 5 12.708385554598923 38.28917684754297 34.145442127939376 14.856995469918735 6 28.599026981138238 38.16904116646169 18.360742570844987 14.87118928155509 7 24.668676556485522 35.47092734284584 22.635023599879553 17.22537250078908 8 24.13059370504674 34.56383204508886 21.85202451806475 19.453549731799654 9 25.180944503096462 18.018263150447872 22.570990106553186 34.229802239902476 10-11 23.13386809463171 28.401031066221154 28.25781734672776 20.20728349241938 12-13 24.697881634178284 26.589409259279417 28.96789981025769 19.744809296284608 14-15 21.493512265292388 27.556591948424643 28.865839124495942 22.08405666178703 16-17 19.487586934990585 30.470782224447372 29.409785333609058 20.63184550695299 18-19 19.168615941089133 28.348088562210954 33.32828441780713 19.15501107889278 20-21 20.066065150904993 27.82382263629339 33.179598529950624 18.930513682850997 22-23 19.82349926760359 28.0298763315783 32.872036969012605 19.274587431805504 24-25 19.682317685942017 28.00821370135359 33.20000580473594 19.109462807968452 26-27 19.466507035993995 28.406731698025933 32.983506342144764 19.14325492383531 28-29 19.227343475058685 28.629996335627496 32.99159733988325 19.151062849430573 30-31 19.478284691065507 28.550020772821526 32.72807923062549 19.24361530548747 32-33 19.241949981542167 28.598755751632858 32.57585171331313 19.58344255351184 34-35 19.648108205184954 28.612931724139184 32.42282525682586 19.316134813850002 36-37 19.695492217065073 28.389281122660215 32.340037625387694 19.575189034887014 38-39 19.441468401689505 28.58502180884484 32.537216675685585 19.436293113780067 40-41 19.599248875401578 28.49261133842195 32.45581059165591 19.45232919452057 42-43 19.358245358506878 28.70216170175671 32.026529563355496 19.913063376380915 44-45 19.4670618392414 28.84959561096931 31.752098219271748 19.931244330517547 46-47 19.61186134584636 28.969845436410836 31.265282255831206 20.1530109619116 48-49 19.65511167285003 29.043112856860088 31.066734947001024 20.23504052328886 50-51 19.446197316607453 29.318383427140997 30.86591481313676 20.369504443114785 52-53 19.73833153447232 29.490488125247932 30.543026062455127 20.22815427782463 54-55 19.404074252914462 29.350178080650018 30.567970171037295 20.67777749539822 56-57 19.173650356998223 29.395164379713307 30.902054427179294 20.529130836109175 58-59 19.668266102347907 28.497390701644104 31.31499051583183 20.519352680176155 60-61 19.88144390058868 29.109798886838284 30.51529320796283 20.493464004610214 62-63 19.46026450721676 29.19348065266035 30.46458830497513 20.88166653514775 64-65 19.001667338687707 29.234277432080468 30.59303132344554 21.17102390578628 66-67 20.529345797157454 28.578164393414795 30.07487724624805 20.8176125631797 68-69 21.411618835278144 27.778337973622246 30.051892808392484 20.75815038270713 70-71 21.340138776568605 27.84467247405285 29.857483049353455 20.95770570002509 72-73 20.57333068825202 27.97601917125132 30.281337926833697 21.169312213662963 74-75 20.46443611458189 28.285991947799527 30.00832986255727 21.24124207506132 76-77 18.111874278300956 29.06265382828278 30.817181842857078 22.008290050559186 78-79 18.44681797952826 29.15336589618348 30.342514298900593 22.05730182538767 80-81 18.60155940890562 29.381027917630103 29.78830370722606 22.22910896623822 82-83 18.813308131013365 29.3563794279412 29.30966927397002 22.52064316707542 84-85 18.781892093391114 29.014934524273816 29.242589424840325 22.960583957494745 86-87 18.80499790484172 28.90710739364124 29.352532601932623 22.935362099584413 88-89 18.882930961610672 28.64615172518879 29.324490769546124 23.146426543654414 90-91 18.294449440633905 28.71803638539383 29.664453086993408 23.323061086978857 92-93 17.53549409418343 28.795523054085802 30.192883878076987 23.476098973653777 94-95 18.33066146361691 27.96781168316685 29.995964427726236 23.705562425490008 96-97 18.671156684649045 27.96895158142077 29.437997970011192 23.921893763918995 98-99 17.92550837218761 28.0491519232061 29.65172398879966 24.373615715806626 100-101 17.829321069900285 28.348291754691047 29.449638149972486 24.372749025436185 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2651.0 1 2057.0 2 1393.0 3 1429.5 4 1584.5 5 1689.5 6 1945.0 7 2374.5 8 2765.0 9 2923.5 10 2838.0 11 2755.0 12 2781.5 13 2786.0 14 2788.5 15 2828.0 16 2765.5 17 2688.5 18 2721.0 19 2809.0 20 2838.5 21 2919.0 22 3096.5 23 3312.0 24 3597.0 25 3969.5 26 4432.5 27 4983.5 28 5576.5 29 6257.5 30 7101.0 31 8028.0 32 9081.0 33 10277.5 34 11494.0 35 12678.0 36 13780.0 37 14807.5 38 15668.0 39 16236.0 40 16771.0 41 17428.0 42 18025.0 43 18533.0 44 18886.0 45 19272.0 46 19710.0 47 20049.0 48 20164.0 49 19728.0 50 18958.0 51 17796.5 52 16543.5 53 15505.5 54 14185.0 55 12666.0 56 11171.0 57 9766.0 58 8518.5 59 7200.0 60 5896.5 61 4629.0 62 3555.0 63 2714.5 64 1948.5 65 1364.5 66 955.5 67 640.0 68 404.5 69 276.5 70 195.0 71 134.0 72 97.5 73 80.0 74 54.0 75 36.0 76 21.5 77 7.0 78 3.5 79 2.0 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023581739751920096 2 0.008162909914126187 3 3.627959961833861E-4 4 0.0 5 0.1327833346031193 6 0.0 7 0.0 8 0.0012697859866418514 9 0.0 10-11 0.0 12-13 0.0 14-15 5.441939942750792E-4 16-17 0.0 18-19 9.069899904584653E-5 20-21 0.0 22-23 2.720969971375396E-4 24-25 0.0 26-27 2.720969971375396E-4 28-29 0.0036279599618338613 30-31 0.013242053860693595 32-33 0.003174464966604629 34-35 0.0045349499522923265 36-37 0.10557363488936536 38-39 0.10521083889318196 40-41 0.1148249327920417 42-43 0.11663891277295864 44-45 0.11400864180062908 46-47 0.10784110986551151 48-49 0.10938299284929093 50-51 0.10711551787314474 52-53 0.08389657411740804 54-55 0.07255919923667722 56-57 0.560882610099515 58-59 1.5946698012240739 60-61 0.5307705424162938 62-63 0.004081454957063094 64-65 0.12625300667181838 66-67 1.8966067690476967 68-69 2.8564742759498905 70-71 2.763145005931715 72-73 2.6173010154659933 74-75 2.004266480915117 76-77 1.424246382016928 78-79 0.9528836839756636 80-81 0.05369380743514115 82-83 3.627959961833861E-4 84-85 1.8139799809169306E-4 86-87 5.441939942750792E-4 88-89 1.8139799809169306E-4 90-91 0.25586187630833307 92-93 0.40615011772730075 94-95 2.0095270228597757 96-97 2.419849294543185 98-99 1.432590689929146 100-101 0.9542441689613512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 551274.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.53943344328485 #Duplication Level Percentage of deduplicated Percentage of total 1 86.1585403743264 57.32940462808062 2 8.745373501761938 11.63824396114311 3 2.062267253128256 4.116662838953803 4 0.8831764301762466 2.350642371775611 5 0.4991649477369751 1.6607076408582606 6 0.3167624266798339 1.2646315444437723 7 0.22182754704606344 1.0332195511791074 8 0.16008007472101227 0.8521309981995888 9 0.13866251763144954 0.8303872826714533 >10 0.7432214724074497 9.020869422025008 >50 0.048912731133933136 2.2364699701746953 >100 0.01902161268559267 2.0668674119750907 >500 8.152119722395266E-4 0.4108059452372339 >1k 0.0016304239444790531 3.0060074117821363 >5k 5.434746481596844E-4 2.182949021500495 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6904 1.252371778825049 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5169 0.9376462521359614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4300 0.7800113917942801 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4299 0.7798299937961884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4236 0.7684019199164118 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1590 0.288422816965792 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1183 0.21459383174247287 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.18448176405925185 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 0.17123971019855827 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 791 0.14348581649052922 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.8139799809169306E-4 0.0 4 0.0 0.0 0.0 1.8139799809169306E-4 0.0 5 0.0 0.0 0.0 1.8139799809169306E-4 0.0 6 0.0 0.0 0.0 1.8139799809169306E-4 0.0 7 0.0 0.0 0.0 1.8139799809169306E-4 0.0 8 0.0 0.0 0.0 1.8139799809169306E-4 0.0 9 0.0 0.0 0.0 1.8139799809169306E-4 0.0 10-11 0.0 0.0 0.0 1.8139799809169306E-4 0.0 12-13 0.0 0.0 0.0 3.627959961833861E-4 0.0 14-15 3.627959961833861E-4 0.0 0.0 0.001360484985687698 0.0 16-17 3.627959961833861E-4 0.0 0.0 0.002176775977100317 0.0 18-19 3.627959961833861E-4 0.0 0.0 0.003265163965650475 0.0 20-21 3.627959961833861E-4 0.0 0.0 0.0039000569589714013 0.0 22-23 3.627959961833861E-4 0.0 0.0 0.005804735938934178 0.0 24-25 5.441939942750792E-4 0.0 0.0 0.00888850190649296 0.0 26-27 5.441939942750792E-4 0.0 0.0 0.014239742850197905 0.0 28-29 5.441939942750792E-4 0.0 0.0 0.028116689704212425 0.0 30-31 5.441939942750792E-4 0.0 0.0 0.06947543326911844 0.0 32-33 5.441939942750792E-4 0.0 0.0 0.167248954240541 0.0 34-35 5.441939942750792E-4 0.0 0.0 0.30148347282839383 0.0 36-37 5.441939942750792E-4 0.0 0.0 0.4883234108628377 0.0 38-39 7.255919923667722E-4 0.0 0.0 0.7294013503266978 0.0 40-41 7.255919923667722E-4 0.0 0.0 0.9876214006102229 0.0 42-43 8.162909914126188E-4 0.0 0.0 1.241669296937639 0.0 44-45 9.069899904584653E-4 0.0 0.0 1.5504086896897005 0.0 46-47 9.069899904584653E-4 0.0 0.0 1.8556108214789742 0.0 48-49 9.069899904584653E-4 0.0 0.0 2.114102968759637 0.0 50-51 9.069899904584653E-4 0.0 0.0 2.3646136041242647 0.0 52-53 9.069899904584653E-4 0.0 0.0 2.602244981624383 0.0 54-55 9.069899904584653E-4 0.0 0.0 2.849399754024315 0.0 56-57 9.069899904584653E-4 0.0 0.0 3.0550143848612485 0.0 58-59 9.069899904584653E-4 0.0 0.0 3.18643723447868 0.0 60-61 9.069899904584653E-4 0.0 0.0 3.2911038793775873 0.0 62-63 9.069899904584653E-4 0.0 0.0 3.3818935774224794 0.0 64-65 9.069899904584653E-4 0.0 0.0 3.478578710405352 0.0 66-67 9.069899904584653E-4 0.0 0.0 3.557577538574284 0.0 68-69 9.069899904584653E-4 0.0 0.0 3.666325638430254 0.0 70-71 9.069899904584653E-4 0.0 0.0 3.829855933709916 0.0 72-73 9.976889895043119E-4 0.0 0.0 4.071296669169959 0.0 74-75 0.0010883879885501583 0.0 0.0 4.39228042679321 0.0 76-77 0.0010883879885501583 0.0 0.0 4.891669115539641 0.0 78-79 0.0010883879885501583 0.0 0.0 5.632407840747069 0.0 80-81 0.0012697859866418514 0.0 0.0 6.5291488443133545 0.0 82-83 0.0012697859866418514 0.0 0.0 7.367479692494113 0.0 84-85 0.0012697859866418514 0.0 0.0 7.987316651973428 0.0 86-87 0.0012697859866418514 0.0 0.0 8.464756182950765 0.0 88-89 0.0012697859866418514 0.0 0.0 8.89829739838991 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4275 0.0 37.75254 1 GGTATCA 2525 0.0 37.456123 1 TAGCCCG 50 1.1842367E-6 33.646214 60-61 TTAGCGG 40 3.8916347E-4 30.425215 64-65 TATCAAC 5350 0.0 30.073338 2 ATCAACG 5420 0.0 29.679543 3 TCAACGC 5505 0.0 29.135836 4 CAACGCA 5700 0.0 28.139082 5 AACGCAG 5845 0.0 27.411154 6 GGGTAGC 95 0.001306183 24.728848 6 CGCAGAG 6525 0.0 23.980627 8 ACGCAGA 6610 0.0 23.883352 7 GCAGAGT 6655 0.0 22.94733 9 GTACATG 2165 0.0 22.820208 1 TACATGG 2095 0.0 22.455631 2 AGTACTT 4345 0.0 21.899393 12-13 GCTTAGC 160 5.4160006E-5 20.557718 8 ACATGGG 2125 0.0 20.363823 3 GAGTACT 4240 0.0 20.169836 12-13 AGAGTAC 5750 0.0 20.02143 10-11 >>END_MODULE