FastQCFastQC Report
Fri 27 May 2016
SRR939698_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939698_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511008
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.556155676623458No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18110.35439758281670736No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.3324801177280982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15670.30664881958795165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.2886451875508798No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.1133054668420064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.10723902561212348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.046.7313161
GTATCAA32250.038.2426451
GTACAGG1905.3244366E-737.244511
TATCAAC45200.029.9922562
GTACATG18700.029.7330111
GTGTAAG1755.2647927E-428.8834971
GTCGGGA856.614976E-427.7854082
GTACATA1857.286474E-427.3222271
ATCAACG50300.027.2356833
TCAACGC51800.026.6267874
CAACGCA52550.026.138945
AACGCAG53750.025.643196
ACCGTGG1151.3361225E-424.6275448
ACGCAGA57050.023.9116697
CAACCGT1000.001713865623.6013976
TACATGG21450.023.1221222
CGCAGAG59850.022.5563898
GCAGAGT58300.022.5083649
GTATATA2250.002279948722.4649411
GAGTACT34550.022.13271312-13
Produced by FastQC (version 0.11.5)