FastQCFastQC Report
Fri 27 May 2016
SRR939698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511008
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65271.2772794163692154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49920.9768927296637234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.7931382678940448No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.7708294195002818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38440.7522387125054794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14850.2906021040766485No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.20077963554386624No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.19040797795729225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.17299142087795102No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.1561619387563404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.040.6060521
GTATCAA37700.038.5584641
ATTGGCG500.001676271337.7129177
TATCAAC48500.029.9634442
ATCAACG49600.029.2989333
TCAACGC50650.028.691554
CAACGCA51800.028.0545755
AACGCAG52950.027.510136
ACGCAGA59350.024.464157
CGCAGAG59000.024.1298758
GCAGAGT58450.023.22789
AGTACTT38000.022.39204412-13
GAGTACT37100.021.91872612-13
CATAGGG1303.0746253E-421.7766282
AGAGTAC50550.021.35573410-11
CGCAGAA1150.003887571420.4961518
TTGCGTG856.6370674E-520.02432864-65
GTATCGT1456.4612745E-419.5066816
GTACTTT41700.019.50083414-15
GTACATG19700.019.4056931
Produced by FastQC (version 0.11.5)