FastQCFastQC Report
Fri 27 May 2016
SRR939697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240950
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA196900.8786452174301077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159680.7125549432160467No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA151040.6739998661282045No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT125820.5614583100917021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105530.4709163524398135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83750.37372542894754457No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT75900.33869564247305833No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67470.30107766795332336No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG65200.29094803543140185No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA64790.28911845422700194No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT60000.26774359088779315No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC51640.23043798389076062No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51480.2297240009817265No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG51220.2285637787545461No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44390.19808563332515228No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG39480.1761752828041679No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37560.1676074878957585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36180.16144938530533925No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34450.15372944510140787No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT32960.14708047926102769No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31800.14190410317053034No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG31050.13855730828443294No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29660.13235458176219908No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA29650.13230995783038443No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG29340.13092661594413083No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29060.12967714585332113No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25400.11334478680916575No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24280.10834690644592694No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT22760.10156406881010285No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22630.10098395769651265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129950.024.8670041
TACATGG130450.024.2988112
AGTACTT118750.022.77533512-13
ACTAACG1050.002211877422.592568
CATTCCG3600.022.4047899
ACATGGG133350.022.3825873
TAGGACG2554.984031E-1022.3292584
GAGTACT115950.021.81122212-13
ATGGGAG35850.021.4416925
CATGGGA103250.021.323634
GTACTTT131300.019.33424214-15
ATGGGAT38000.018.6052675
GTACCGA2051.607242E-518.5140516
CATGGGG27600.018.0515064
GTCGGGA3951.8189894E-1118.0196692
TACTTTT151650.017.83489214-15
AGTACAT97700.017.7762092
TAGACTG6700.017.7051015
GCTTATA24450.017.0787641
ACTTTTT155700.017.0673716-17
Produced by FastQC (version 0.11.5)