##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939697_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2240950 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.555342153997188 31.0 31.0 34.0 30.0 34.0 2 31.733988263905932 31.0 31.0 34.0 30.0 34.0 3 31.77929226444142 31.0 31.0 34.0 30.0 34.0 4 35.542547580267296 37.0 35.0 37.0 33.0 37.0 5 35.304403489591465 37.0 35.0 37.0 33.0 37.0 6 35.35101363261117 37.0 35.0 37.0 33.0 37.0 7 35.28850442892523 37.0 35.0 37.0 32.0 37.0 8 35.27194493406814 37.0 35.0 37.0 32.0 37.0 9 36.85404716749593 39.0 37.0 39.0 33.0 39.0 10-11 36.74168098351146 39.0 35.0 39.0 32.0 39.0 12-13 36.69151743680136 39.0 35.0 39.0 32.0 39.0 14-15 37.629539927263 39.0 36.0 41.0 32.0 41.0 16-17 37.63030567393293 39.0 36.5 41.0 32.0 41.0 18-19 37.71862067426761 39.5 37.0 41.0 32.0 41.0 20-21 37.67547937258752 39.0 36.5 41.0 32.0 41.0 22-23 37.53718445302216 39.0 36.0 41.0 32.0 41.0 24-25 37.39624199558223 39.0 36.0 41.0 32.0 41.0 26-27 37.07046989000201 39.0 36.0 40.5 31.0 41.0 28-29 36.871375309578525 39.0 36.0 40.0 30.5 41.0 30-31 36.63515540284254 38.0 35.0 40.0 30.0 41.0 32-33 36.43217273924006 38.0 35.0 40.0 30.0 41.0 34-35 36.38113969521854 38.0 35.0 40.0 30.0 41.0 36-37 36.20956357794685 38.0 35.0 40.0 30.0 41.0 38-39 36.1676797786653 38.0 35.0 40.0 30.0 41.0 40-41 36.04937704991187 38.0 35.0 40.0 30.0 41.0 42-43 35.86616769673576 38.0 35.0 40.0 29.5 41.0 44-45 35.61232356812959 38.0 35.0 40.0 28.0 41.0 46-47 35.21671300118254 38.0 34.0 40.0 26.5 41.0 48-49 34.85772417947746 38.0 34.0 40.0 24.5 41.0 50-51 34.589834891452284 38.0 34.0 40.0 24.0 41.0 52-53 34.3140636783507 38.0 33.0 40.0 24.0 41.0 54-55 34.077951092170736 38.0 33.0 40.0 23.0 41.0 56-57 32.22421562283853 37.0 30.5 39.5 14.5 41.0 58-59 27.561438898681363 33.0 17.5 38.5 4.0 40.0 60-61 28.29554385416899 33.5 20.0 38.0 6.5 40.0 62-63 31.139037461790757 35.5 29.5 39.0 4.5 40.0 64-65 31.289947566880116 36.0 30.5 39.0 2.0 40.0 66-67 24.77066668154131 27.5 18.0 35.5 2.0 39.0 68-69 17.44953791918606 13.0 7.5 28.0 2.0 35.5 70-71 16.340698141413238 13.0 7.5 27.0 2.0 34.0 72-73 17.50104196880787 13.5 7.0 29.0 2.0 34.5 74-75 18.51080635444789 17.5 8.5 30.0 2.0 34.5 76-77 19.669030545081327 21.5 8.0 30.0 2.0 34.5 78-79 22.751827350007808 27.5 10.5 33.5 2.0 36.0 80-81 26.197252281398512 31.5 22.5 35.0 2.0 36.0 82-83 27.35701242776501 33.0 26.0 35.0 2.0 36.0 84-85 27.373062094201117 34.0 26.0 35.0 2.0 36.0 86-87 27.123381601552914 34.0 26.0 35.0 2.0 36.0 88-89 26.656744461054465 33.0 25.5 35.0 2.0 35.5 90-91 25.46793279635869 32.5 17.5 35.0 2.0 35.0 92-93 23.237095205158525 31.5 2.0 34.5 2.0 35.0 94-95 16.082442267788217 7.0 2.0 32.0 2.0 35.0 96-97 13.534507463352597 2.0 2.0 30.0 2.0 34.0 98-99 13.2381556928981 2.0 2.0 30.5 2.0 34.0 100-101 12.953237912492469 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37959389825375567 1101 2 0.41608820168927707 1101 3 0.4713847641547204 1101 4 0.2549995214424001 1101 5 0.4828600111210477 1101 6 0.3462175693454128 1101 7 0.4011735068540858 1101 8 0.47698048579373875 1101 9 0.4717599266884278 1101 10-11 0.3902399727186108 1101 12-13 0.4204840175192146 1101 14-15 0.6014928205077439 1101 16-17 0.6015537513956772 1101 18-19 0.4716919559451398 1101 20-21 0.4719994899148716 1101 22-23 0.5611563144520346 1101 24-25 0.5123363614826175 1101 26-27 0.3527946630738725 1101 28-29 0.4841993462881362 1101 30-31 0.5446681532324718 1101 32-33 0.3888557210912751 1101 34-35 0.45680278305563604 1101 36-37 0.3119978838198847 1101 38-39 0.6395017494731903 1101 40-41 0.619426228127594 1101 42-43 0.5892277138329334 1101 44-45 0.4478916482047808 1101 46-47 0.5230531222855959 1101 48-49 0.4431577821714754 1101 50-51 0.43272622714147957 1101 52-53 0.5439467546251535 1101 54-55 0.3459225583278567 1101 56-57 -1.7231223223826646 1101 58-59 -9.616982590190013 1101 60-61 -9.860222154404237 1101 62-63 -3.6994923105459634 1101 64-65 -4.900174562485763 1101 66-67 -9.816402103403462 1101 68-69 -4.63270420379593 1101 70-71 -4.325853227376371 1101 72-73 -4.886886635126004 1101 74-75 -6.905492126712785 1101 76-77 -7.86943890960616 1101 78-79 -11.130811154344148 1101 80-81 -8.142234445719648 1101 82-83 -4.164412178089403 1101 84-85 -3.4048760763955173 1101 86-87 -3.2408370815872907 1101 88-89 -3.262869247325778 1101 90-91 -9.563976137928513 1101 92-93 -16.637057245534557 1101 94-95 -11.216223724742967 1101 96-97 -9.18834574087825 1101 98-99 -9.22680487195985 1101 100-101 -9.055291172751186 1102 1 0.30216933254736134 1102 2 0.3668133552733508 1102 3 0.38281014886015186 1102 4 0.2778579499303362 1102 5 0.3100816765612464 1102 6 0.33891925221001173 1102 7 0.47052688492048844 1102 8 0.37848954156444137 1102 9 0.4972759678855567 1102 10-11 0.459499570608358 1102 12-13 0.26021021421737345 1102 14-15 0.36620270902900387 1102 16-17 0.4372839551837018 1102 18-19 0.4437700506800937 1102 20-21 0.4439679205917386 1102 22-23 0.4350800726915196 1102 24-25 0.4347385961924175 1102 26-27 0.5067358021962178 1102 28-29 0.5158256773247558 1102 30-31 0.5183052618090258 1102 32-33 0.6170746766977828 1102 34-35 0.4995828626665215 1102 36-37 0.48963211676114327 1102 38-39 0.37707326501742244 1102 40-41 0.5535836230317841 1102 42-43 0.5112530871367866 1102 44-45 0.5837362594565789 1102 46-47 0.7448306200041941 1102 48-49 0.4071468470982538 1102 50-51 0.6660704543514839 1102 52-53 0.5862568454156829 1102 54-55 0.29886884426296234 1102 56-57 -2.7112775161725438 1102 58-59 -10.331836021130162 1102 60-61 -10.577660088681522 1102 62-63 -4.264174086772975 1102 64-65 -5.145611427973659 1102 66-67 -6.629702525914709 1102 68-69 -4.142617051331992 1102 70-71 -4.2780860441716815 1102 72-73 -4.609410022509504 1102 74-75 -5.255921666032354 1102 76-77 -7.727863698557245 1102 78-79 -9.805598473523563 1102 80-81 -5.563471064637483 1102 82-83 -3.2435854938648845 1102 84-85 -2.781801017875381 1102 86-87 -2.635714725655184 1102 88-89 -2.8037867786485755 1102 90-91 -8.908339992829038 1102 92-93 -13.117398222423729 1102 94-95 -10.281980600396544 1102 96-97 -9.139933193395235 1102 98-99 -9.074766099622869 1102 100-101 -9.036030769787995 1103 1 0.2413737398618494 1103 2 0.2426191644449105 1103 3 0.28654395120347687 1103 4 0.23744696268154541 1103 5 0.29108366812941 1103 6 0.34864974389456904 1103 7 0.2573393412741112 1103 8 0.22579032008654565 1103 9 0.4451532665949216 1103 10-11 0.474244934392857 1103 12-13 0.39850173324072813 1103 14-15 0.5503613361401989 1103 16-17 0.4159260677978267 1103 18-19 0.38092559991321195 1103 20-21 0.3186341389400269 1103 22-23 0.48390129711798124 1103 24-25 0.4869386324027545 1103 26-27 0.4212239433130449 1103 28-29 0.5043598055610587 1103 30-31 0.5570139755663703 1103 32-33 0.5789054968617648 1103 34-35 0.5396269616759071 1103 36-37 0.2986099508661013 1103 38-39 0.3140610828277275 1103 40-41 0.4660265271002757 1103 42-43 0.4453350259413327 1103 44-45 0.46759951373068986 1103 46-47 0.5416592416838384 1103 48-49 0.38675086127200586 1103 50-51 0.44712637344726147 1103 52-53 0.5047007064714677 1103 54-55 0.45336125302586083 1103 56-57 -2.3631215272425585 1103 58-59 -10.241303858597508 1103 60-61 -10.516075498763513 1103 62-63 -4.069607446832979 1103 64-65 -5.373373888206956 1103 66-67 -6.254685713975643 1103 68-69 -3.840872360025058 1103 70-71 -4.045965024074949 1103 72-73 -4.161411936484104 1103 74-75 -4.67384539155805 1103 76-77 -6.419315344834047 1103 78-79 -8.872086718098481 1103 80-81 -4.706460615370741 1103 82-83 -2.447815377733182 1103 84-85 -1.980717811709276 1103 86-87 -1.8428357457519162 1103 88-89 -1.8326663275713209 1103 90-91 -4.6302887552116765 1103 92-93 -9.512912021146022 1103 94-95 -8.299873417818887 1103 96-97 -7.857764970546517 1103 98-99 -8.131428929718826 1103 100-101 -8.328001646336633 1104 1 0.23971514534348515 1104 2 0.23972729411887173 1104 3 0.17377177135230326 1104 4 0.16387406283185157 1104 5 0.194061270237448 1104 6 0.3411040481124985 1104 7 0.2831407107558235 1104 8 0.24609928852166263 1104 9 0.18787738468952142 1104 10-11 0.29286654548231894 1104 12-13 0.29850368169031327 1104 14-15 0.41353610279016806 1104 16-17 0.4224075389700488 1104 18-19 0.4035974810021159 1104 20-21 0.41032891895078905 1104 22-23 0.474294048885497 1104 24-25 0.42309202177720806 1104 26-27 0.5132637844680161 1104 28-29 0.4232656119408347 1104 30-31 0.6289992601327938 1104 32-33 0.6346071242810893 1104 34-35 0.5681731399312184 1104 36-37 0.44593073989540244 1104 38-39 0.4502432350294825 1104 40-41 0.2937494390117976 1104 42-43 0.404051461577545 1104 44-45 0.5232989143504838 1104 46-47 0.49252388938558767 1104 48-49 0.4186443831410358 1104 50-51 0.6019001677280968 1104 52-53 0.4712451775137936 1104 54-55 0.41121127807736 1104 56-57 -2.3828428989510684 1104 58-59 -9.704232007199263 1104 60-61 -9.88604218955384 1104 62-63 -3.5260287944178295 1104 64-65 -4.615214709153715 1104 66-67 -5.775837062117194 1104 68-69 -3.4578733249715228 1104 70-71 -3.735892189173578 1104 72-73 -3.6521493782625747 1104 74-75 -4.440327326646845 1104 76-77 -6.077041689728182 1104 78-79 -8.837109171660433 1104 80-81 -4.262367850613554 1104 82-83 -1.9015736586439402 1104 84-85 -1.4090954026833167 1104 86-87 -1.4615193360950265 1104 88-89 -1.3363644848947054 1104 90-91 -3.8632748006012747 1104 92-93 -6.527919722161069 1104 94-95 -7.791905743525431 1104 96-97 -7.819146885222981 1104 98-99 -8.04704686658906 1104 100-101 -8.325952805200222 1105 1 0.3034759854423541 1105 2 0.3383860062987125 1105 3 0.25201934537452786 1105 4 0.2220575694660667 1105 5 0.18923942541560024 1105 6 0.1692374170909403 1105 7 0.27284340243034677 1105 8 0.19371674149122953 1105 9 0.3260419431357704 1105 10-11 0.38900843176504907 1105 12-13 0.31313384026413615 1105 14-15 0.5007220623704924 1105 16-17 0.4740436024371064 1105 18-19 0.5386919639839292 1105 20-21 0.5296995952228727 1105 22-23 0.5189204084414314 1105 24-25 0.46242358223553026 1105 26-27 0.5506721038481643 1105 28-29 0.6058480998754447 1105 30-31 0.4936862163690421 1105 32-33 0.5060077424422715 1105 34-35 0.3990177882406556 1105 36-37 0.443125001033323 1105 38-39 0.45623977370207314 1105 40-41 0.4651282948976885 1105 42-43 0.375784142042626 1105 44-45 0.4995178128229014 1105 46-47 0.6087137089556975 1105 48-49 0.4933681570197379 1105 50-51 0.5910274723483653 1105 52-53 0.7246409835856582 1105 54-55 0.6738103691271533 1105 56-57 1.9288302166657019 1105 58-59 -8.289767785974476 1105 60-61 -9.261357390784536 1105 62-63 -3.133912272442153 1105 64-65 -4.29078430387824 1105 66-67 -5.6735414234835275 1105 68-69 -3.741104084822 1105 70-71 -3.584314283511164 1105 72-73 -1.8608911529198426 1105 74-75 -3.940574966331104 1105 76-77 -6.111926429401654 1105 78-79 -8.559623746287683 1105 80-81 -4.184924542747712 1105 82-83 -1.7771034809343291 1105 84-85 -1.2649987481641247 1105 86-87 -1.2110249478541597 1105 88-89 -1.3151746887753326 1105 90-91 -3.5995792473282577 1105 92-93 -5.799663096157968 1105 94-95 -7.829806528186779 1105 96-97 -8.079002428740496 1105 98-99 -8.303600453846872 1105 100-101 -8.330710676436967 1106 1 0.20353024081041937 1106 2 0.1880636926291146 1106 3 0.15600573555338215 1106 4 0.11749919896627148 1106 5 0.0034764018283084397 1106 6 0.23409557148884375 1106 7 0.1789981681667001 1106 8 0.19522117847683518 1106 9 0.1906315794408897 1106 10-11 0.34343852050476187 1106 12-13 0.2807038434826765 1106 14-15 0.3577205450593439 1106 16-17 0.36847369117681694 1106 18-19 0.3374321359458534 1106 20-21 0.24804342712319283 1106 22-23 0.3481006006341829 1106 24-25 0.27625410317902066 1106 26-27 0.3046092963627629 1106 28-29 0.23982759318547409 1106 30-31 0.3763015090261774 1106 32-33 0.2690028226758443 1106 34-35 0.4244102200765951 1106 36-37 0.24741761218785996 1106 38-39 0.1591953118877356 1106 40-41 0.06972620744539171 1106 42-43 -0.06335880705848496 1106 44-45 -0.26189098758171525 1106 46-47 -0.14764934221907566 1106 48-49 -0.10764062499323046 1106 50-51 -0.08904517466989148 1106 52-53 -0.22735405066878656 1106 54-55 -0.2677234944987674 1106 56-57 0.9127844214567347 1106 58-59 -9.111469013617128 1106 60-61 -10.220121471891712 1106 62-63 -4.547049428173452 1106 64-65 -5.822714047774511 1106 66-67 -7.25145213202185 1106 68-69 -4.195720848811149 1106 70-71 -4.158489647895733 1106 72-73 -4.525515733360322 1106 74-75 -5.250157016080518 1106 76-77 -6.978460982714449 1106 78-79 -9.548404839671287 1106 80-81 -5.147964682524254 1106 82-83 -2.8652997198418646 1106 84-85 -2.375575786188861 1106 86-87 -2.2571043078247364 1106 88-89 -2.166138871036587 1106 90-91 -4.691094259512724 1106 92-93 -6.671056723451176 1106 94-95 -7.241865933216895 1106 96-97 -7.197612939568581 1106 98-99 -6.70440600812523 1106 100-101 -7.402938365066329 1107 1 0.035433376710091835 1107 2 0.052008543829295206 1107 3 0.028640680616550895 1107 4 0.12711120463464454 1107 5 -0.09120518744280304 1107 6 -0.13962630326703618 1107 7 0.004051725822577623 1107 8 0.026750765789621767 1107 9 -0.017478750729750914 1107 10-11 0.09730562626914008 1107 12-13 0.00473222910390092 1107 14-15 0.09530876983075842 1107 16-17 0.11309674172012762 1107 18-19 0.08198979004036744 1107 20-21 0.0649854077500649 1107 22-23 0.20046570010167386 1107 24-25 0.23468346369081416 1107 26-27 0.17027913793796046 1107 28-29 0.10700931414010029 1107 30-31 0.23894606507716531 1107 32-33 0.05133252408889177 1107 34-35 0.08101321299469788 1107 36-37 -0.0021617003453329175 1107 38-39 0.05824380628082082 1107 40-41 -0.011357373542004723 1107 42-43 -0.03581125277116115 1107 44-45 -0.06970303869679384 1107 46-47 0.16860012309498273 1107 48-49 -0.012076947046054443 1107 50-51 -0.003786781086581925 1107 52-53 0.056682956087399816 1107 54-55 0.16621667716564303 1107 56-57 1.4122242258068383 1107 58-59 -8.751920864909167 1107 60-61 -10.406432264824929 1107 62-63 -3.9538666135459195 1107 64-65 -4.929588003371595 1107 66-67 -6.375606272215045 1107 68-69 -3.6512966848996573 1107 70-71 -3.8454885030761883 1107 72-73 -2.139914351943041 1107 74-75 -4.302663112327295 1107 76-77 -6.312447389922614 1107 78-79 -7.442590077529875 1107 80-81 -2.28265872002212 1107 82-83 -1.0596009664203194 1107 84-85 -0.8584663448501146 1107 86-87 -0.8307643740303696 1107 88-89 -0.6340521161585073 1107 90-91 -0.3622571228796936 1107 92-93 -2.7942389502740532 1107 94-95 -5.737211970764635 1107 96-97 -6.235569216571653 1107 98-99 -5.852709122955542 1107 100-101 -6.831756620701302 1108 1 -0.033578302189415155 1108 2 -0.10530012892045448 1108 3 -0.055173503322272666 1108 4 0.047769572460993004 1108 5 -3.590442493361934E-4 1108 6 -0.11472465324420966 1108 7 -0.03559088036205793 1108 8 -0.026299237544279208 1108 9 0.010864507360082598 1108 10-11 -0.03304720267114192 1108 12-13 0.09711709423492465 1108 14-15 -0.012930060330241133 1108 16-17 -7.712461957751771E-4 1108 18-19 0.02711127213733988 1108 20-21 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13.113455282088 2316 98-99 12.735417326125962 2316 100-101 11.54504497240857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 29.0 5 142.0 6 310.0 7 645.0 8 1307.0 9 2375.0 10 4035.0 11 6573.0 12 9715.0 13 13402.0 14 16538.0 15 21946.0 16 29118.0 17 39316.0 18 40493.0 19 36638.0 20 37255.0 21 37231.0 22 37585.0 23 31766.0 24 32779.0 25 38342.0 26 53094.0 27 78847.0 28 109632.0 29 148649.0 30 185990.0 31 207938.0 32 214524.0 33 200132.0 34 180953.0 35 163997.0 36 133677.0 37 88418.0 38 34925.0 39 2623.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99794504742593 19.991019625154156 14.759103230122998 21.251932097296915 2 14.392157002752617 23.01135856506853 40.24146099386998 22.355023438308873 3 19.993297458523003 27.89149736650809 31.38998304730022 20.725222127668687 4 11.561792989580312 17.715210067159017 40.373502309288476 30.349494633972203 5 11.626035333731044 40.3341220526364 34.741364864230796 13.298477749401757 6 27.411142595774113 38.93201543988041 18.740668020259264 14.916173944086214 7 24.969276422945626 35.77058836654098 22.111292085945692 17.148843124567705 8 24.033454146916576 34.250204603649046 21.323805690346546 20.392535559087836 9 25.751400075860687 17.889645016622417 22.08014458153908 34.27881032597782 10-11 23.288359847386154 27.045204042928223 28.855909324170554 20.810526785515073 12-13 26.823177670184524 25.179901381110692 28.23284767620875 19.764073272496038 14-15 23.01818921766417 26.03598128109461 29.28550614629645 21.660323354944772 16-17 19.700194114103393 29.338985698029852 29.850398268591444 21.110421919275307 18-19 19.24732646271997 29.2608252322123 32.052370763227124 19.439477541840606 20-21 21.23554742408354 27.417055266739553 30.812110935094488 20.53528637408242 22-23 20.303492906108232 27.59109785081452 30.369304515690278 21.736104727386966 24-25 20.890988395767064 27.440288145716806 31.314181778750484 20.354541679765653 26-27 21.405185227825964 26.44899937213932 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52-53 20.76499486400786 28.914831852083427 29.85563396007324 20.46453932383547 54-55 19.75415979814069 28.04767745022454 30.542875343035565 21.655287408599204 56-57 20.391068472323006 28.90759945447961 29.605274081812798 21.096057991384583 58-59 20.124025136123766 27.822722747466315 30.8252921473425 21.22795996906742 60-61 20.744530154891184 27.701746487744707 30.395131664570453 21.15859169279366 62-63 21.8785129015948 27.61926628636404 29.565650845416936 20.936569966624226 64-65 20.814507638215137 28.359432960568746 30.278888734249804 20.547170666966313 66-67 20.678911073621627 27.84622617080429 30.007618243189434 21.46724451238465 68-69 21.049150980868106 28.258779817728445 29.69842994902575 20.993639252377694 70-71 20.961624577172238 28.127157196626108 29.331719717724436 21.579498508477222 72-73 20.244995906111345 27.532461636560363 30.029286608662076 22.19325584866621 74-75 20.53931095688082 28.481571180864023 29.552853640068776 21.426264222186383 76-77 19.217066704337512 27.990864819844386 30.697822991848472 22.09424548396963 78-79 19.548219390211294 27.995502603844592 30.566434475411537 21.889843530532577 80-81 20.18939156319478 28.92400472652904 29.35546905085662 21.53113465941956 82-83 20.376341315012485 28.75832043140738 28.81753653426044 22.047801719319686 84-85 20.516121546936674 28.11253734473626 28.62781103507672 22.743530073250348 86-87 20.646876008923936 28.39101635720197 28.976663582394796 21.985444051479302 88-89 21.21353633386607 27.527475555529772 28.969299356321088 22.28968875428307 90-91 20.06329811874985 27.72318022382276 29.92022754933296 22.293294108094432 92-93 19.20289757672046 28.938266095682042 30.022325928992522 21.83651039860498 94-95 18.71580336997525 28.9454215976702 29.601760014650086 22.737015017704465 96-97 18.533028155576595 28.64779927454696 28.97892964789399 23.840242921982455 98-99 18.957378483306485 28.554260838538315 28.87326112018228 23.615099557972925 100-101 19.740526979665646 28.326390458054046 29.06351948018887 22.86956308209144 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2542.0 1 1810.0 2 1066.5 3 1116.0 4 1239.0 5 1389.5 6 1777.0 7 2442.5 8 3122.5 9 3596.0 10 3777.0 11 3909.5 12 4186.5 13 4495.0 14 4796.5 15 5063.5 16 5360.0 17 5747.5 18 6107.0 19 6574.0 20 7186.5 21 7987.5 22 9098.0 23 10532.0 24 12197.5 25 14401.0 26 17336.5 27 20759.0 28 24391.5 29 28333.0 30 32767.5 31 37555.0 32 43142.0 33 49678.5 34 55913.0 35 61422.0 36 66448.0 37 70755.0 38 73109.0 39 73905.0 40 74808.0 41 76065.0 42 78130.0 43 80905.5 44 84998.5 45 91036.5 46 99816.5 47 110905.5 48 120760.5 49 112485.5 50 90850.0 51 74154.5 52 61337.5 53 52036.0 54 45048.0 55 39101.0 56 33561.0 57 28458.0 58 23898.0 59 19795.5 60 15807.5 61 11967.5 62 8741.0 63 6257.5 64 4409.5 65 3022.5 66 2049.5 67 1394.0 68 950.0 69 685.5 70 497.0 71 374.5 72 290.5 73 217.0 74 150.0 75 95.0 76 52.5 77 32.0 78 17.5 79 8.5 80 4.5 81 3.0 82 2.0 83 1.5 84 1.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022713581293647783 2 0.007764564135746001 3 4.016153863316897E-4 4 0.0 5 0.14034226555701823 6 0.0 7 0.0 8 0.0014279658180682301 9 0.0 10-11 0.0 12-13 0.0 14-15 3.123675227024253E-4 16-17 0.0 18-19 2.2311965907316096E-4 20-21 0.0 22-23 3.5699145451705753E-4 24-25 2.2311965907316096E-5 26-27 3.123675227024253E-4 28-29 0.0031459871929315694 30-31 0.011892277828599479 32-33 0.002922867533858408 34-35 0.004239273522390057 36-37 0.10120707735558579 38-39 0.10031459871929316 40-41 0.11307704321827797 42-43 0.11584372699078516 44-45 0.11080122269573171 46-47 0.10457618420759054 48-49 0.1034382739463174 50-51 0.10323746625315157 52-53 0.08255427385706955 54-55 0.06999263705125058 56-57 0.5631093955688435 58-59 1.6133782547580269 60-61 0.5325196903099132 62-63 0.004596264976907115 64-65 0.12331823556973605 66-67 1.945291059595261 68-69 2.933309533903032 70-71 2.8400901403422654 72-73 2.689060443115643 74-75 2.0510944019277537 76-77 1.4433833865101855 78-79 0.9568932818670652 80-81 0.055846850666012186 82-83 2.4543162498047704E-4 84-85 2.2311965907316096E-4 86-87 4.6855128405363797E-4 88-89 1.5618376135121266E-4 90-91 0.2523260224458377 92-93 0.4023293692407238 94-95 2.041143265133091 96-97 2.47917177982552 98-99 1.4497422967937705 100-101 0.960686316071309 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2240950.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.06448625288837 #Duplication Level Percentage of deduplicated Percentage of total 1 68.51100092871971 28.13369055809033 2 14.989715719969196 12.310899502347594 3 6.3312528566070005 7.799689376810953 4 3.2208687982626056 5.290532899544474 5 1.8683374226145157 3.836115820335533 6 1.2256807359919653 3.01991698401433 7 0.8071940004056396 2.320290485314995 8 0.5592901221042429 1.8373569224420752 9 0.40777455139123386 1.5070547213894743 >10 1.885814819527959 13.209483926482632 >50 0.11597692517096019 3.2901881099508272 >100 0.06696877910696794 5.237934102247775 >500 0.004136827149071562 1.1582907421291608 >1k 0.0043545548937595335 4.2441006632773455 >5k 9.79774851095895E-4 2.7963042922007357 >10k+ 6.5318323406393E-4 4.00815089342178 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19784 0.8828398670206832 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16660 0.7434347040317723 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16648 0.7428992168499966 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13952 0.6225930966777483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12039 0.5372275151163569 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10576 0.47194270287155005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9509 0.42432896762533745 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7955 0.35498337758539905 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7611 0.3396327450411656 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7609 0.3395434971775363 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7037 0.3140186081795667 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6384 0.2848791807046119 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6043 0.2696624199558223 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5262 0.23481112920859457 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5141 0.22941163345902407 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4258 0.19000870166670386 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4218 0.18822374439411857 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3873 0.17282848791807046 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3860 0.17224837680448024 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3833 0.17104353064548516 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3816 0.17028492380463642 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3749 0.16729512037305605 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3601 0.1606907784644905 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3469 0.15480041946495907 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3341 0.14908855619268613 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3262 0.1455632655793302 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2857 0.12749057319440416 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2739 0.12222494924027756 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2733 0.12195720564938976 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2657 0.11856578683147771 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2611 0.11651308596800465 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2421 0.10803453892322454 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2399 0.10705281242330263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.4623931814632185E-5 0.0 0.0 0.0 0.0 9 4.4623931814632185E-5 0.0 0.0 8.924786362926437E-5 0.0 10-11 4.4623931814632185E-5 0.0 0.0 1.7849572725852877E-4 0.0 12-13 4.4623931814632185E-5 0.0 0.0 3.5699145451705753E-4 0.0 14-15 4.4623931814632185E-5 0.0 0.0 5.577991476829024E-4 0.0 16-17 4.4623931814632185E-5 0.0 0.0 0.0011155982953658047 0.0 18-19 6.693589772194827E-5 0.0 0.0 0.0014725897498828622 0.0 20-21 8.924786362926437E-5 0.0 0.0 0.002432004283897454 0.0 22-23 8.924786362926437E-5 0.0 0.0 0.005087128226868069 0.0 24-25 8.924786362926437E-5 0.0 0.0 0.007206764988063098 0.0 26-27 8.924786362926437E-5 0.0 0.0 0.008701666703853276 0.0 28-29 1.1155982953658047E-4 0.0 0.0 0.015239072714696891 0.0 30-31 1.3387179544389658E-4 0.0 0.0 0.02907249157723287 0.0 32-33 1.3387179544389658E-4 0.0 0.0 0.05537829938195854 0.0 34-35 1.3387179544389658E-4 0.0 0.0 0.09716861152636158 0.0 36-37 1.7849572725852874E-4 0.0 0.0 0.15678618443071018 0.0 38-39 1.7849572725852874E-4 0.0 0.0 0.2355697360494433 0.0 40-41 1.7849572725852874E-4 0.0 0.0 0.326602556951293 0.0 42-43 2.2311965907316093E-4 0.0 0.0 0.4178138735804011 0.0 44-45 2.2311965907316093E-4 0.0 0.0 0.5196233740154845 0.0 46-47 2.6774359088779315E-4 0.0 0.0 0.643320913005645 0.0 48-49 2.6774359088779315E-4 0.0 0.0 0.7642071442914835 0.0 50-51 2.6774359088779315E-4 0.0 0.0 0.8811664695776344 0.0 52-53 2.6774359088779315E-4 0.0 0.0 0.9888886409781565 0.0 54-55 2.6774359088779315E-4 0.0 0.0 1.0993998081170933 0.0 56-57 2.6774359088779315E-4 0.0 0.0 1.1936009281777817 0.0 58-59 2.6774359088779315E-4 0.0 0.0 1.262790334456369 0.0 60-61 2.6774359088779315E-4 0.0 0.0 1.3289676253374685 0.0 62-63 2.9005555679510926E-4 0.0 0.0 1.3849929717307392 0.0 64-65 3.123675227024253E-4 0.0 0.0 1.433833865101854 0.0 66-67 3.569914545170575E-4 0.0 0.0 1.4739730917691158 0.0 68-69 3.569914545170575E-4 0.0 0.0 1.5303777415828108 0.0 70-71 3.569914545170575E-4 0.0 0.0 1.621789865905085 0.0 72-73 3.569914545170575E-4 0.0 0.0 1.7531404092014546 0.0 74-75 3.793034204243736E-4 0.0 0.0 1.924050068051496 0.0 76-77 4.016153863316897E-4 0.0 0.0 2.1625203596688904 0.0 78-79 4.016153863316897E-4 0.0 0.0 2.5360003569914547 0.0 80-81 4.016153863316897E-4 0.0 0.0 2.9878399785805128 0.0 82-83 4.4623931814632186E-4 0.0 0.0 3.436310493317566 0.0 84-85 4.4623931814632186E-4 2.2311965907316092E-5 0.0 3.824962627457105 0.0 86-87 4.4623931814632186E-4 4.4623931814632185E-5 0.0 4.130123385171467 0.0 88-89 4.4623931814632186E-4 4.4623931814632185E-5 0.0 4.3820254802650656 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13320 0.0 24.908133 1 TACATGG 13045 0.0 24.741148 2 CATTCCG 285 0.0 24.72446 9 ACATGGG 12980 0.0 23.448814 3 CATGGGA 10105 0.0 22.20566 4 AGTACTT 12130 0.0 21.822952 12-13 GAGTACT 11880 0.0 21.748367 12-13 GCTTACG 520 0.0 21.712751 60-61 ATGGGAT 4000 0.0 20.34547 5 GTACTTT 13045 0.0 19.842113 14-15 ATGGGAG 3545 0.0 18.71046 5 GTAGGAC 565 0.0 18.317472 3 CATGGGG 2600 0.0 18.27383 4 TGCGTTA 465 0.0 18.081541 60-61 TACTTTT 14605 0.0 17.91571 14-15 TAGGACG 185 1.6394407E-4 17.799503 4 ACTTTTT 14875 0.0 17.637888 16-17 GCGTGCG 565 0.0 17.460281 9 GCTTATA 2795 0.0 17.174126 1 TAGGCCG 85 0.0015717618 17.091702 94-95 >>END_MODULE