FastQCFastQC Report
Fri 27 May 2016
SRR939696_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939696_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235572
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA83880.3752059875503898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.30265184928063155No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA62570.27988362709856807No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53610.23980439905312823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44810.20044087150849982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.15226528154763075No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30270.13540158849726155No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29440.13168889214930227No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA27860.12462134970378945No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG27710.12395038048427873No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT25590.11446734884852736No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG110100.030.4753041
GTATAGC7950.025.8137151
TACATGG131900.024.7435422
GTATCGA2501.337875E-424.6262821
ACATGGG129750.023.5534613
GTATAGG5303.0195224E-1023.2323421
GAGTACT106650.023.09742212-13
CATGGGA102300.022.6224334
GCTTATA22850.022.4528471
AGTACTT113550.022.0471312-13
GTACTTT118650.020.66520714-15
GTCCTAC5551.2989403E-820.3370191
ATGGGAG35600.020.018185
CTTTGCG6150.019.9676462
CATTCCG3850.019.6109459
ATGGGAT39400.019.4051255
CATGGGG25850.019.367084
GTACTAA5452.3901703E-718.8274331
ATAGCCC8300.018.7799193
TTGCGTT6300.018.7421024
Produced by FastQC (version 0.11.5)