##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939695_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1120900 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.521083950397003 31.0 31.0 34.0 30.0 34.0 2 31.6799678829512 31.0 31.0 34.0 30.0 34.0 3 31.69789365688286 31.0 31.0 34.0 30.0 34.0 4 35.47921045588367 37.0 35.0 37.0 33.0 37.0 5 35.25167097867785 37.0 35.0 37.0 33.0 37.0 6 35.32574538317424 37.0 35.0 37.0 32.0 37.0 7 35.271481844946024 37.0 35.0 37.0 32.0 37.0 8 35.262687126416274 37.0 35.0 37.0 32.0 37.0 9 36.79050851993934 39.0 37.0 39.0 32.0 39.0 10-11 36.68353956641984 39.0 35.0 39.0 32.0 39.0 12-13 36.638186724953165 39.0 35.0 39.0 32.0 39.0 14-15 37.45393879917923 39.0 36.0 41.0 32.0 41.0 16-17 37.484835846195026 39.0 36.0 41.0 32.0 41.0 18-19 37.608802747791955 39.0 36.0 41.0 32.0 41.0 20-21 37.56926041573736 39.0 36.0 41.0 32.0 41.0 22-23 37.42232045677581 39.0 36.0 41.0 32.0 41.0 24-25 37.23717459184584 39.0 36.0 41.0 31.5 41.0 26-27 36.890188241591574 39.0 35.5 40.0 30.5 41.0 28-29 36.69595369792131 38.5 35.0 40.0 30.5 41.0 30-31 36.38115844410741 38.0 35.0 40.0 30.0 41.0 32-33 36.03745918458382 38.0 35.0 40.0 30.0 41.0 34-35 35.87734945133375 38.0 35.0 40.0 30.0 41.0 36-37 35.617748684093144 38.0 35.0 40.0 29.5 41.0 38-39 35.48458693906682 38.0 34.5 40.0 28.5 41.0 40-41 35.35275448300473 38.0 34.5 40.0 27.5 41.0 42-43 35.07033678294228 38.0 34.0 40.0 26.5 41.0 44-45 34.70501784280489 38.0 34.0 40.0 24.5 41.0 46-47 34.164820679810866 38.0 33.0 40.0 23.5 41.0 48-49 33.6557587652779 38.0 33.0 40.0 20.5 41.0 50-51 33.32533990543313 37.0 33.0 40.0 18.5 41.0 52-53 33.077755375144974 37.0 33.0 40.0 16.0 41.0 54-55 32.826476046034436 37.0 32.5 40.0 13.0 41.0 56-57 30.90629404942457 36.0 28.5 39.5 5.0 40.5 58-59 26.122577393166203 32.0 14.0 37.5 2.0 40.0 60-61 26.75999152466768 32.0 17.5 37.0 2.0 40.0 62-63 29.676654028013203 34.5 28.0 38.0 2.0 40.0 64-65 29.874357212953875 35.0 28.5 38.0 2.0 40.0 66-67 23.51266036220894 26.0 16.0 34.5 2.0 38.5 68-69 16.4904697118387 13.0 7.0 28.0 2.0 34.5 70-71 15.399731465786424 12.0 6.5 25.5 2.0 33.5 72-73 16.477006869479883 13.0 6.5 28.0 2.0 34.0 74-75 17.44197653671157 16.0 6.5 29.0 2.0 34.5 76-77 18.517346774913015 20.5 7.0 30.0 2.0 34.0 78-79 21.404991078597554 26.5 8.5 33.0 2.0 35.0 80-81 24.743176911410472 30.5 16.5 34.0 2.0 36.0 82-83 25.91576634846998 32.5 23.0 35.0 2.0 36.0 84-85 25.93162280310465 33.0 23.0 35.0 2.0 36.0 86-87 25.748297350343474 33.0 21.0 35.0 2.0 35.0 88-89 25.29197207601035 33.0 19.0 35.0 2.0 35.0 90-91 24.014138192523866 32.0 4.0 34.5 2.0 35.0 92-93 21.79901686145062 30.5 2.0 34.0 2.0 35.0 94-95 15.032004193059148 4.0 2.0 31.5 2.0 34.5 96-97 12.660858238915157 2.0 2.0 29.0 2.0 34.0 98-99 12.398548041752164 2.0 2.0 29.0 2.0 34.0 100-101 12.145193148362923 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37937489151541826 1101 2 0.3635501665773013 1101 3 0.39092856302714196 1101 4 0.06212987867017006 1101 5 0.4686106260301841 1101 6 0.2819216529130415 1101 7 0.34888415789893656 1101 8 0.24630890719091525 1101 9 0.34599148850658423 1101 10-11 0.32539347908411287 1101 12-13 0.21942416459974368 1101 14-15 0.5644890872731239 1101 16-17 0.5726986903914124 1101 18-19 0.3905471856096199 1101 20-21 0.3123419905243381 1101 22-23 0.7012611725860154 1101 24-25 0.3026066537040819 1101 26-27 0.3160315494557224 1101 28-29 0.4486456634481186 1101 30-31 0.5459395447179887 1101 32-33 0.46221780900038567 1101 34-35 0.4613466199415228 1101 36-37 0.19526184572531236 1101 38-39 0.22645381607534176 1101 40-41 0.321959146705737 1101 42-43 0.40999344698727924 1101 44-45 0.15117401155431054 1101 46-47 0.27842220809254314 1101 48-49 0.3162366415014404 1101 50-51 0.11580344360391592 1101 52-53 0.28809367978834644 1101 54-55 0.4477937815881958 1101 56-57 -1.9702988994144128 1101 58-59 -9.379536136809882 1101 60-61 -10.46381155034354 1101 62-63 -4.36066760708805 1101 64-65 -4.86298687528949 1101 66-67 -9.22637919659353 1101 68-69 -4.675604939043772 1101 70-71 -4.10714813361586 1101 72-73 -4.597086087489831 1101 74-75 -6.621849327756507 1101 76-77 -8.098549357886878 1101 78-79 -10.623915494308665 1101 80-81 -7.420012619092294 1101 82-83 -3.9569091699599177 1101 84-85 -3.102985262438846 1101 86-87 -3.1105699062289 1101 88-89 -3.1494896855941867 1101 90-91 -9.444562663617269 1101 92-93 -15.976306579388892 1101 94-95 -10.632821962379305 1101 96-97 -9.03628010276919 1101 98-99 -9.071648326937828 1101 100-101 -8.963561767246102 1102 1 0.41283017462438565 1102 2 0.4171942547758043 1102 3 0.4562960107248948 1102 4 0.2811540436694031 1102 5 0.2276848338499704 1102 6 0.32147837139849855 1102 7 0.33798760998530497 1102 8 0.4373398761193741 1102 9 0.5625371920462854 1102 10-11 0.5657785877930621 1102 12-13 0.5147794173199358 1102 14-15 0.5566097109929515 1102 16-17 0.6052196172097126 1102 18-19 0.6133590948537346 1102 20-21 0.555780776867536 1102 22-23 0.8073268912806455 1102 24-25 0.6295218374377995 1102 26-27 0.5451284107289709 1102 28-29 0.6743316802685015 1102 30-31 0.5359171675261436 1102 32-33 0.580775486508351 1102 34-35 0.38613439181256126 1102 36-37 0.29672287507613504 1102 38-39 0.6240347508657536 1102 40-41 0.45164622121349396 1102 42-43 0.5476299498307995 1102 44-45 0.7096240654253236 1102 46-47 0.8671199502457299 1102 48-49 0.6860601892532614 1102 50-51 0.6227760897561865 1102 52-53 0.5050231896607471 1102 54-55 0.43843147235671864 1102 56-57 -1.951785423766875 1102 58-59 -9.419153040864217 1102 60-61 -9.465470106758136 1102 62-63 -3.51853139431924 1102 64-65 -4.197377895997274 1102 66-67 -5.601569126462543 1102 68-69 -3.557051208130492 1102 70-71 -4.116232207922343 1102 72-73 -4.324102106717641 1102 74-75 -4.9466155906865374 1102 76-77 -6.8145247784952065 1102 78-79 -9.331345550784434 1102 80-81 -5.449470001789631 1102 82-83 -3.0528952858398277 1102 84-85 -2.564308634567201 1102 86-87 -2.401366842092788 1102 88-89 -2.619597190833293 1102 90-91 -7.888644350233971 1102 92-93 -12.142008303656107 1102 94-95 -9.466722618698 1102 96-97 -8.489706338941309 1102 98-99 -8.416465264143559 1102 100-101 -8.306321541974476 1103 1 0.137749199553042 1103 2 0.18774729275306612 1103 3 0.203688974551401 1103 4 0.2718611241037152 1103 5 0.20625074635429286 1103 6 0.27817962570188826 1103 7 0.12296708148522839 1103 8 0.15823468538118135 1103 9 0.3564836457178089 1103 10-11 0.33592512998622226 1103 12-13 0.32764181433019957 1103 14-15 0.4206234448095145 1103 16-17 0.4205936766673588 1103 18-19 0.380132370209715 1103 20-21 0.31300186427616694 1103 22-23 0.41303292538184166 1103 24-25 0.45483837635305946 1103 26-27 0.29782989467007326 1103 28-29 0.2574040476295778 1103 30-31 0.3366772868636758 1103 32-33 0.31866811606384005 1103 34-35 0.43414534034594254 1103 36-37 0.3805267496569087 1103 38-39 0.315875760086449 1103 40-41 0.30936065931409473 1103 42-43 0.16900459079039365 1103 44-45 0.2653996390103188 1103 46-47 0.1983931802297434 1103 48-49 0.2790648505283855 1103 50-51 0.1647970127145797 1103 52-53 0.2575880947222231 1103 54-55 0.2903373727495122 1103 56-57 -2.931825462212675 1103 58-59 -9.886050950235106 1103 60-61 -9.992926628376491 1103 62-63 -4.133508124981983 1103 64-65 -5.045951271727159 1103 66-67 -6.5930155847638545 1103 68-69 -4.034778665207684 1103 70-71 -3.941185771103271 1103 72-73 -4.274131367006429 1103 74-75 -4.945222023135088 1103 76-77 -6.34350403887659 1103 78-79 -9.096906746456135 1103 80-81 -5.243772023527434 1103 82-83 -2.9956557718569314 1103 84-85 -2.6118005833579936 1103 86-87 -2.372062570275819 1103 88-89 -2.023193798862092 1103 90-91 -4.829560326907227 1103 92-93 -9.576895364497137 1103 94-95 -7.954530986029445 1103 96-97 -7.402582440137461 1103 98-99 -7.877535190678012 1103 100-101 -7.948685810346882 1104 1 0.1919903886347818 1104 2 0.2571248209506294 1104 3 0.10686741255230459 1104 4 0.2118536737943444 1104 5 0.2321861697930956 1104 6 0.20923875982754936 1104 7 0.22975536440531386 1104 8 0.24921424649303958 1104 9 0.13588802285184443 1104 10-11 0.2603346311400827 1104 12-13 0.2840926693382144 1104 14-15 0.408243101878881 1104 16-17 0.2015391240182467 1104 18-19 0.10001078487043458 1104 20-21 0.17131691962021733 1104 22-23 0.13057693553404448 1104 24-25 0.2663714398058872 1104 26-27 0.21359116326299699 1104 28-29 0.3220509385165471 1104 30-31 0.2678620097106261 1104 32-33 0.3946489060130247 1104 34-35 0.31571401689586054 1104 36-37 0.08461189958278936 1104 38-39 0.3350804009181658 1104 40-41 0.1622247777276442 1104 42-43 0.5307256122556296 1104 44-45 0.6110650119199406 1104 46-47 0.5343144140751761 1104 48-49 0.4545416625997447 1104 50-51 0.420792425055204 1104 52-53 0.25926016363604276 1104 54-55 0.20738930697039137 1104 56-57 -2.8065721594225153 1104 58-59 -10.248081886021783 1104 60-61 -10.01806678538944 1104 62-63 -4.0521927125795365 1104 64-65 -5.634952327445273 1104 66-67 -6.159153696805998 1104 68-69 -4.246449760325877 1104 70-71 -4.112422707944123 1104 72-73 -4.055126663418996 1104 74-75 -4.893565256723926 1104 76-77 -6.642648258008428 1104 78-79 -8.828680151943086 1104 80-81 -5.028458855492044 1104 82-83 -2.940813689047207 1104 84-85 -2.465564689869595 1104 86-87 -2.2244463601338147 1104 88-89 -2.1905374853431816 1104 90-91 -4.185337717227696 1104 92-93 -6.199311135039974 1104 94-95 -7.4622444520772095 1104 96-97 -7.379806828926137 1104 98-99 -7.612656598260942 1104 100-101 -7.742578102368095 1105 1 0.15559623029409408 1105 2 0.22911494019763623 1105 3 0.21868995435207594 1105 4 0.12193001874968701 1105 5 0.23303865113927458 1105 6 0.14594588074940162 1105 7 0.15332855850002858 1105 8 0.06302506204125535 1105 9 0.34139780129163455 1105 10-11 0.3771423355946908 1105 12-13 0.29429271674976576 1105 14-15 0.44878538503086673 1105 16-17 0.43030836179645604 1105 18-19 0.2541431187002843 1105 20-21 0.16251528137345872 1105 22-23 0.40570409347397884 1105 24-25 0.4638699012469374 1105 26-27 0.48420341320373694 1105 28-29 0.5598592583947308 1105 30-31 0.4738528532579025 1105 32-33 0.4675793029187645 1105 34-35 0.5826584349576507 1105 36-37 0.3304435051742658 1105 38-39 0.501270249440573 1105 40-41 0.5602768115211205 1105 42-43 0.5590161786692533 1105 44-45 0.2864518523569046 1105 46-47 0.3722804121153942 1105 48-49 0.31811916543168905 1105 50-51 0.44656454227447995 1105 52-53 0.45433862821212045 1105 54-55 0.25680967370724517 1105 56-57 1.5230358149845138 1105 58-59 -8.530362474388347 1105 60-61 -9.420859588408337 1105 62-63 -3.8454972592239365 1105 64-65 -4.620846618213996 1105 66-67 -6.444026091004492 1105 68-69 -4.237438862618234 1105 70-71 -4.045051971991727 1105 72-73 -2.514261611061661 1105 74-75 -4.173697653872129 1105 76-77 -5.696333734089144 1105 78-79 -8.490211081804102 1105 80-81 -4.467925730337125 1105 82-83 -2.240919554257861 1105 84-85 -1.7604135867788102 1105 86-87 -1.8249507449303053 1105 88-89 -1.7845899608208313 1105 90-91 -4.1311240733564105 1105 92-93 -6.336608506633041 1105 94-95 -7.799333259167013 1105 96-97 -7.617153001987573 1105 98-99 -8.04317782419707 1105 100-101 -7.890164707600508 1106 1 0.1489188529570562 1106 2 0.21328461736627702 1106 3 0.14328857407889828 1106 4 0.12123376520544582 1106 5 0.15517429644216918 1106 6 0.2428375674461023 1106 7 0.2569434997052866 1106 8 0.2820966520180832 1106 9 0.4196084836560914 1106 10-11 0.431912481969853 1106 12-13 0.4157655718570652 1106 14-15 0.19738073398523426 1106 16-17 0.3421774157552022 1106 18-19 0.36351834358838175 1106 20-21 0.4902388561226658 1106 22-23 0.5419278085533463 1106 24-25 0.6121345747454043 1106 26-27 0.4789068487741943 1106 28-29 0.5248932075016555 1106 30-31 0.2763687213619406 1106 32-33 0.2743091241597142 1106 34-35 0.42969944734988275 1106 36-37 0.3054858740914952 1106 38-39 0.3941335606569112 1106 40-41 0.1955834329462931 1106 42-43 0.24414990612647358 1106 44-45 0.37091982109566146 1106 46-47 0.01978226519329951 1106 48-49 0.06367132867526237 1106 50-51 0.005287771595568813 1106 52-53 0.1699703298773727 1106 54-55 0.04081275377460969 1106 56-57 1.2566036916090972 1106 58-59 -8.81527860012418 1106 60-61 -9.958260727601473 1106 62-63 -4.25767710853092 1106 64-65 -5.300521412036591 1106 66-67 -6.483633832192474 1106 68-69 -4.406751712544111 1106 70-71 -3.9281902223983245 1106 72-73 -4.419774427884011 1106 74-75 -4.839313793281159 1106 76-77 -6.4701129515505365 1106 78-79 -9.233454543857384 1106 80-81 -5.131688342061533 1106 82-83 -2.643624418317504 1106 84-85 -2.195023774749277 1106 86-87 -2.039050059111567 1106 88-89 -1.9496312862572935 1106 90-91 -3.9064667128589967 1106 92-93 -5.962910158930857 1106 94-95 -7.327250417588981 1106 96-97 -6.810235174298629 1106 98-99 -6.2821996869181795 1106 100-101 -6.8679448641945875 1107 1 0.00754122733492224 1107 2 -0.05899626444909245 1107 3 -0.06439743385069008 1107 4 0.11182440716478936 1107 5 -0.02332055776022912 1107 6 -0.0719049317806224 1107 7 0.027159384191975278 1107 8 -0.028236418870200453 1107 9 0.09995041206483535 1107 10-11 0.07434633175755323 1107 12-13 0.040275244831782686 1107 14-15 -0.05609988491644202 1107 16-17 -0.10100088753754477 1107 18-19 0.12580200216611104 1107 20-21 -0.006739217999317759 1107 22-23 0.020467653335849434 1107 24-25 0.12682241744709444 1107 26-27 0.15102393675889658 1107 28-29 0.06177933212556752 1107 30-31 0.20786353338566954 1107 32-33 0.24926466081297605 1107 34-35 -0.023076260548769767 1107 36-37 0.03682620674059223 1107 38-39 -0.10942350708203463 1107 40-41 -0.2564901758606055 1107 42-43 -0.4834400398848331 1107 44-45 -0.2895770436232894 1107 46-47 -0.4361594790352541 1107 48-49 -0.6746056375536824 1107 50-51 -0.5829473674534498 1107 52-53 -0.5226280167188122 1107 54-55 -0.7266140662349514 1107 56-57 0.08055520019949114 1107 58-59 -8.74229720091714 1107 60-61 -10.556938602077755 1107 62-63 -4.36357456515719 1107 64-65 -5.699949891073789 1107 66-67 -6.747326961287321 1107 68-69 -3.781124304845097 1107 70-71 -4.008510709983737 1107 72-73 -2.42355996341251 1107 74-75 -4.377078640172591 1107 76-77 -6.727676114107126 1107 78-79 -7.518657915338981 1107 80-81 -3.0020996543805154 1107 82-83 -1.9372960362912401 1107 84-85 -1.7971058192899498 1107 86-87 -1.7335631415286876 1107 88-89 -1.422375957685464 1107 90-91 -1.2733604999410773 1107 92-93 -3.6847611698129548 1107 94-95 -5.199757482516013 1107 96-97 -5.912946383982852 1107 98-99 -5.622539365797914 1107 100-101 -6.409787861129605 1108 1 0.022278482394096244 1108 2 -0.016705940667602448 1108 3 0.0811238322961465 1108 4 0.012018269433802686 1108 5 -0.02022219771475875 1108 6 -0.03472031591140734 1108 7 -0.12335734322965664 1108 8 0.13267069619006122 1108 9 -0.057511853517887346 1108 10-11 0.09367457179168781 1108 12-13 -0.030519561835703257 1108 14-15 -0.01836904707062814 1108 16-17 -0.0728106791570795 1108 18-19 0.052414967743388274 1108 20-21 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2.0756446366714414 2316 86-87 2.1946366950148395 2316 88-89 2.4565862844560833 2316 90-91 3.352292262165193 2316 92-93 5.05466037914989 2316 94-95 11.413626873496293 2316 96-97 12.997527082218781 2316 98-99 12.747207540780988 2316 100-101 11.549450731031822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 26.0 5 146.0 6 333.0 7 610.0 8 1050.0 9 1818.0 10 3087.0 11 4815.0 12 6816.0 13 9277.0 14 11004.0 15 14436.0 16 19125.0 17 25120.0 18 25175.0 19 21111.0 20 20840.0 21 20937.0 22 20598.0 23 18683.0 24 18425.0 25 20911.0 26 28797.0 27 42172.0 28 58746.0 29 77643.0 30 94018.0 31 101739.0 32 101654.0 33 92573.0 34 83256.0 35 72794.0 36 56122.0 37 33985.0 38 12235.0 39 810.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.19897808942342 19.63278323395613 15.521850789633836 18.646387886986613 2 15.0733562210929 24.398839433563637 38.02942813909667 22.498376206246785 3 21.272159707805447 28.476300677047046 31.987863291631612 18.2636763235159 4 11.648318315639218 18.17619769827817 39.382638950843074 30.79284503523954 5 11.00611371346223 41.01369889938863 33.60156957062844 14.378617816520695 6 26.21830671781604 38.68186278883041 17.88643054688197 17.213399946471586 7 23.708983852261575 37.24078865197609 21.704255508966007 17.345971986796325 8 24.97071500007583 31.157723255189445 20.800067090083605 23.07149465465112 9 25.64742617539477 18.5295744491034 22.807030065126238 33.015969310375596 10-11 24.007627799090017 26.8559193505219 27.116736550985816 22.019716299402265 12-13 28.66723168882148 25.707645641894906 27.062405210099026 18.562717459184583 14-15 23.62656136948196 26.49517214430306 30.46910894993358 19.409157536281405 16-17 18.764296547417253 29.801543402622894 30.31488089927737 21.119279150682488 18-19 18.28593910515617 29.358162796932284 33.55604137569871 18.79985672221283 20-21 21.26228923186725 26.092381122312432 32.30765456329735 20.33767508252297 22-23 19.513291804112328 26.82181020120038 32.78984028423652 20.87505771045078 24-25 20.983960196235344 26.869325425395154 33.02590153082481 19.120812847544695 26-27 20.761112625273388 25.500078731481935 35.16464693279543 18.574161710449253 28-29 18.733363875180498 28.499112070884447 33.976035504674286 18.791488549260773 30-31 19.23042948726528 27.569629401608736 33.237371236354065 19.96256987477192 32-33 19.323438975549895 27.738341803654198 33.38032045514463 19.55789876565128 34-35 20.802161198615885 26.551722138234098 33.00516255605631 19.64095410709371 36-37 20.425311680441975 26.314318210336463 34.451835832299196 18.808534276922362 38-39 20.335668769899215 26.914455146668097 33.96733961177274 18.782536471659945 40-41 19.38726201694408 27.861490846891428 33.634715268876754 19.11653186728774 42-43 18.746516413942917 28.597383201612097 31.81041074159295 20.84568964285204 44-45 19.68725088481249 27.458907856688292 31.70158652182165 21.15225473667757 46-47 20.749008510222136 28.336132562440074 30.88526941593984 20.029589511397948 48-49 20.56536477602929 27.826768059953565 31.056256483515405 20.55161068050174 50-51 19.220043271388413 28.779358342022594 32.260628618508456 19.739969768080538 52-53 19.901126336486552 29.794653875208276 30.026457388692346 20.27776239961283 54-55 18.651346524107616 28.097125977801895 31.211092714123158 22.04043478396733 56-57 20.03423612880536 28.468516398531506 30.258735775319124 21.23851169734401 58-59 20.44545580232587 27.713724272513605 31.093331761900767 20.747488163259757 60-61 20.447744394114086 28.192999520613398 31.352466643616644 20.00678944165587 62-63 20.46747495096362 28.277563977029963 30.81161955888001 20.443341513126402 64-65 19.500462460324655 30.012982831132533 30.641535859544256 19.845018848998553 66-67 20.246445402787643 28.35873165167449 29.503043159100233 21.891779786437635 68-69 20.568991820030565 28.718551079216663 29.478943251031396 21.23351384972137 70-71 20.896638889169346 28.736171294668793 29.102810394945898 21.264379421215963 72-73 20.302119372207585 27.70910757245962 30.44761141024172 21.541161645091076 74-75 19.70036914346696 29.94742391765569 29.52269039480322 20.829516544074128 76-77 18.288880642942477 28.726830901784744 30.49867863736741 22.48560981790537 78-79 18.802284802228055 28.722655400284005 30.911484405364593 21.56357539212335 80-81 19.89261756947947 29.406183192819746 29.561811844201753 21.139387393499035 82-83 19.30449607658849 28.848616669023404 30.096766034778316 21.750121219609785 84-85 19.323507291914794 28.921249818226265 29.472013089493345 22.2832298003656 86-87 19.4184917327262 29.448418697371874 29.15392202038459 21.979167549517328 88-89 19.491586663918874 28.802015168181967 28.573270205433314 23.133127962465842 90-91 18.92628686729919 28.164353740036347 29.641415876014253 23.26794351665021 92-93 18.295980111721537 29.749206515551936 30.384173215404548 21.57064015732198 94-95 18.27787065568158 29.354607836709718 29.55192785390124 22.815593653707467 96-97 17.706198966678848 29.477957446263687 29.019049086641584 23.79679450041588 98-99 18.01251399520113 29.63298857846771 28.649952979744636 23.704544446586528 100-101 18.191821321567488 28.834723891232006 29.191806908529454 23.781647878671045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5512.0 1 3980.5 2 2419.0 3 2349.0 4 2321.5 5 2662.0 6 3522.5 7 4661.5 8 5683.5 9 6172.0 10 6056.5 11 5996.5 12 6129.0 13 6110.0 14 6056.5 15 5938.5 16 5874.0 17 5850.5 18 5703.5 19 5616.0 20 5688.0 21 5811.0 22 6057.5 23 6460.5 24 6996.5 25 7773.5 26 8797.5 27 9970.5 28 11317.0 29 12757.0 30 14279.0 31 15752.5 32 17356.5 33 19312.0 34 21160.5 35 22794.5 36 24303.5 37 25442.0 38 26404.0 39 27282.5 40 27956.0 41 29142.5 42 30781.5 43 33246.5 44 36892.5 45 42084.0 46 49566.5 47 58454.5 48 65354.0 49 61017.0 50 48439.0 51 38032.0 52 30793.0 53 26337.0 54 23249.5 55 20718.0 56 18683.5 57 16725.0 58 14765.0 59 12999.0 60 11141.0 61 9069.0 62 7089.0 63 5202.0 64 3639.5 65 2535.0 66 1697.5 67 1151.0 68 786.5 69 527.5 70 372.5 71 276.5 72 198.5 73 142.0 74 101.0 75 68.0 76 49.0 77 22.5 78 8.5 79 5.0 80 2.0 81 1.0 82 0.5 83 0.5 84 0.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022660362208939244 2 0.0060665536622357035 3 2.676420733339281E-4 4 0.0 5 0.14390222142920867 6 0.0 7 0.0 8 0.0016950664644482113 9 0.0 10-11 0.0 12-13 0.0 14-15 8.029262200017842E-4 16-17 0.0 18-19 4.460701222232135E-5 20-21 0.0 22-23 2.2303506111160675E-4 24-25 8.92140244446427E-5 26-27 1.3382103666696404E-4 28-29 0.0029886698188955306 30-31 0.012936033544473192 32-33 0.0022749576233383885 34-35 0.003613167990008029 36-37 0.10736907841912749 38-39 0.10661075921134802 40-41 0.11910072263359799 42-43 0.12168792934249265 44-45 0.11709340708359355 46-47 0.11120528147024712 48-49 0.11004549915246678 50-51 0.1090641448835757 52-53 0.0879650281024177 54-55 0.07578731376572397 56-57 0.5609777857079133 58-59 1.5944776518868764 60-61 0.5293514140422875 62-63 0.004326880185565171 64-65 0.1199482558658221 66-67 1.9224730127576057 68-69 2.8948612721919886 70-71 2.8021232937817824 72-73 2.6563475778392363 74-75 2.023552502453386 76-77 1.4256401106253904 78-79 0.9540993844232314 80-81 0.053974484789008834 82-83 4.460701222232135E-5 84-85 8.92140244446427E-5 86-87 4.014631100008921E-4 88-89 8.92140244446427E-5 90-91 0.2530109733250067 92-93 0.39820679810866266 94-95 2.023730930502275 96-97 2.447631367650995 98-99 1.4325095905076277 100-101 0.9629761798554733 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1120900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.399592791155335 #Duplication Level Percentage of deduplicated Percentage of total 1 69.89398668968117 26.839006274341887 2 14.317514067987322 10.995734199847021 3 6.02729583685612 6.943371173013024 4 3.040714670337734 4.670488205402446 5 1.7896517944530823 3.43609500724794 6 1.1648743639188268 2.683842073640342 7 0.7689197798594586 2.0668344504967613 8 0.539925408642644 1.658633266358054 9 0.3931891579600207 1.3588473200005842 >10 1.887781026817444 12.326654423876986 >50 0.10304502879295847 2.73414245363419 >100 0.05500068389403654 3.8702247944006416 >500 0.006962111845848348 1.8176600247001296 >1k 0.008354534215018016 8.001732065395599 >5k 0.001624492764031281 4.458555981555958 >10k+ 0.001160351974308058 6.138178286088392 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18055 1.610759211348024 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16486 1.4707824069943796 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12955 1.1557676866803461 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10896 0.9720760103488268 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10488 0.9356766883754126 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9136 0.8150593273262556 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8257 0.7366401998394148 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7960 0.7101436345793559 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7253 0.6470693192969935 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6414 0.5722187527879383 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5863 0.5230618253189401 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5149 0.45936301186546524 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4959 0.44241234722098316 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4837 0.43152823623873676 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4736 0.4225176197698278 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4163 0.37139798376304756 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4037 0.3601570166830226 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4020 0.35864037826746364 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3912 0.3490052636274422 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2976 0.26550093674725667 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2970 0.2649656526005888 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2938 0.26211080381836027 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2906 0.2592559550361317 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2758 0.24605227941832458 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2672 0.23837987331608526 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2666 0.23784458916941745 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2646 0.23606030868052458 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2588 0.23088589526273529 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2487 0.22187527879382637 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2339 0.20867160317601927 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2299 0.20510304219823355 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2278 0.2032295476848961 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2193 0.19564635560710142 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1974 0.1761084842537247 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1877 0.16745472388259433 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1812 0.16165581229369258 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1763 0.1572843250959051 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1681 0.14996877509144438 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1668 0.14880899277366402 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1519 0.13551610313141227 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1515 0.1351592470336337 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1512 0.13489160496029975 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1440 0.1284681952002855 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1314 0.11722722812026051 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1139 0.10161477384244805 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2303506111160675E-4 0.0 12-13 0.0 0.0 0.0 8.475332322241056E-4 0.0 14-15 0.0 0.0 0.0 0.001025961281113391 0.0 16-17 0.0 0.0 0.0 0.002676420733339281 0.0 18-19 0.0 0.0 0.0 0.003702382014452672 0.0 20-21 0.0 0.0 0.0 0.005486662503345526 0.0 22-23 0.0 0.0 0.0 0.00927825854224284 0.0 24-25 0.0 0.0 0.0 0.014140422874475868 0.0 26-27 0.0 0.0 0.0 0.018734945133374967 0.0 28-29 0.0 0.0 0.0 0.03037737532340084 0.0 30-31 0.0 0.0 0.0 0.06030868052457846 0.0 32-33 0.0 0.0 0.0 0.10928717994468731 0.0 34-35 0.0 0.0 0.0 0.17954322419484342 0.0 36-37 0.0 0.0 0.0 0.27321794986171827 0.0 38-39 0.0 0.0 0.0 0.39771612097421716 0.0 40-41 0.0 0.0 0.0 0.5409046302078686 0.0 42-43 0.0 0.0 0.0 0.6847176376126327 0.0 44-45 0.0 0.0 0.0 0.837452047461861 0.0 46-47 0.0 0.0 0.0 1.021099116781158 0.0 48-49 0.0 0.0 0.0 1.1818181818181819 0.0 50-51 0.0 0.0 0.0 1.3354447319118565 0.0 52-53 0.0 0.0 0.0 1.4756445713266126 0.0 54-55 0.0 0.0 0.0 1.6200374698902666 0.0 56-57 0.0 0.0 0.0 1.7520296190561155 0.0 58-59 0.0 0.0 0.0 1.835489338924079 0.0 60-61 0.0 0.0 0.0 1.9068159514675709 0.0 62-63 0.0 0.0 0.0 1.972388259434383 0.0 64-65 0.0 0.0 0.0 2.028860736907842 0.0 66-67 0.0 0.0 0.0 2.0811847622446247 0.0 68-69 0.0 0.0 0.0 2.152734409849228 0.0 70-71 0.0 0.0 0.0 2.2638504772950307 0.0 72-73 0.0 0.0 0.0 2.4169417432420377 0.0 74-75 0.0 0.0 0.0 2.624408957088054 0.0 76-77 0.0 0.0 0.0 2.901596931037559 0.0 78-79 0.0 0.0 0.0 3.3860290837719687 0.0 80-81 0.0 0.0 0.0 3.9615933624765813 0.0 82-83 0.0 0.0 0.0 4.486127219198858 0.0 84-85 0.0 0.0 0.0 4.943036845392095 0.0 86-87 0.0 0.0 0.0 5.276295833705058 0.0 88-89 0.0 4.460701222232135E-5 0.0 5.5645909536979214 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8635 0.0 26.813759 1 TACATGG 8455 0.0 26.65626 2 GCTTACG 490 0.0 25.986326 60-61 ACATGGG 8480 0.0 25.355854 3 ATGGGAG 1965 0.0 24.662245 5 TACGCGG 100 1.5666956E-8 24.365839 64-65 CATGGGA 6645 0.0 24.215256 4 ATGGGAT 2475 0.0 22.81203 5 GAGTACT 9720 0.0 22.23161 12-13 AGTACTT 9750 0.0 21.392311 12-13 CATGGGG 1730 0.0 21.213211 4 CAATTCG 115 9.169162E-8 20.622282 78-79 GTACTTT 10590 0.0 20.471756 14-15 CGTCACT 260 1.6580088E-8 19.91014 1 GTCGTAC 110 1.0678177E-6 19.906672 70-71 GTCGGGA 525 0.0 19.716995 2 AATCGCG 75 6.777114E-4 19.372993 92-93 ACTTTTT 11630 0.0 19.045015 16-17 TACTTTT 11145 0.0 18.65145 14-15 ATTACGC 65 0.006762625 18.627882 92-93 >>END_MODULE