FastQCFastQC Report
Fri 27 May 2016
SRR939694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111748
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72500.6521262012614369No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67690.6088610008742988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.4224878299758579No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42170.3793125780302731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.32966103829285054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35720.3212958332283935No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30970.2785703234905752No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30710.2762316640101894No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29970.26957547933524506No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24360.2191144036238428No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22670.20391311700133485No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20710.18628322245688772No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18300.16460564804254202No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17830.16037807128953682No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17700.15920874154934392No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15660.14085925947247038No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15040.13528245609616568No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13930.12529817908374918No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11970.10766828453930208No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11860.10667885168221575No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC11800.10613916103289596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG76950.031.9954681
TTTGCGT4000.028.3438593
TTGCGTT4500.026.2407654
TACATGG91100.026.1866972
ACATGGG89650.025.5563683
GTCCTAT2401.1351798E-425.2259351
ATGGGAG22000.024.8846765
CTTTGCG4600.024.6468332
GTATTAC2050.001343052324.6106661
GAGTACT86200.024.22826212-13
CATGGGA71850.023.797474
CATGGGG16650.023.5457674
AGTACTT90300.023.12819712-13
GCTTTGC6600.022.9326691
GTCCAAG4003.0072988E-722.703341
GTACTTT96100.021.8811414-15
GTCGGGA5250.021.5953222
CGGGAGT5050.021.512234
ACGCCGT550.002971028221.47310380-81
GTATAGG2853.6360198E-421.2428911
Produced by FastQC (version 0.11.5)