##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939694_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1111748 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.345721332532193 2.0 2.0 31.0 2.0 33.0 2 22.628087480256315 16.0 16.0 31.0 16.0 34.0 3 27.137022958440223 28.0 25.0 31.0 16.0 34.0 4 32.384928059236444 35.0 32.0 35.0 26.0 37.0 5 33.04514962023768 35.0 33.0 35.0 28.0 37.0 6 33.53625911627455 35.0 33.0 37.0 28.0 37.0 7 33.73041372685177 35.0 33.0 37.0 28.0 37.0 8 33.68334730532459 35.0 33.0 37.0 28.0 37.0 9 34.97995498979984 37.0 34.0 39.0 28.0 39.0 10-11 34.84019579976757 37.0 34.0 39.0 27.0 39.0 12-13 34.877187995840785 37.0 34.0 39.0 27.0 39.0 14-15 35.34994621083195 38.0 34.0 40.0 27.0 41.0 16-17 35.085946635388595 38.0 33.0 40.0 26.5 41.0 18-19 35.51625638184193 38.0 34.0 40.0 27.0 41.0 20-21 35.57124366313229 38.0 34.0 40.0 27.0 41.0 22-23 35.3986555406441 38.0 34.0 40.0 26.5 41.0 24-25 35.1132095582812 38.0 34.0 40.0 25.5 41.0 26-27 35.082988680888114 38.0 34.0 40.0 25.5 41.0 28-29 34.8110983784095 38.0 34.0 40.0 25.0 41.0 30-31 34.602731014582446 38.0 33.5 40.0 24.5 41.0 32-33 34.135721854233154 37.0 33.0 40.0 24.0 41.0 34-35 33.75950665078777 37.0 33.0 40.0 22.0 41.0 36-37 33.3530759668558 37.0 32.5 40.0 19.5 41.0 38-39 33.06055598930693 37.0 31.5 40.0 18.0 41.0 40-41 32.66039111381356 36.5 31.0 40.0 15.5 41.0 42-43 32.29929759261991 36.0 31.0 39.5 15.0 41.0 44-45 31.79204280106643 36.0 30.0 39.0 9.0 41.0 46-47 31.222839618330774 35.5 30.0 39.0 7.0 40.0 48-49 30.673129612106344 35.0 28.5 39.0 2.0 40.0 50-51 29.939436814817746 34.5 27.5 38.5 2.0 40.0 52-53 29.97920751825054 35.0 28.0 38.5 2.0 40.0 54-55 30.12337103372347 35.0 28.0 39.0 2.0 40.0 56-57 29.792252830677455 35.0 27.5 39.0 2.0 40.0 58-59 29.409431813684396 35.0 26.5 38.0 2.0 40.0 60-61 28.744209569075004 34.0 26.0 38.0 2.0 40.0 62-63 28.38559547667277 34.0 26.0 38.0 2.0 40.0 64-65 27.81430144241321 33.5 24.0 37.0 2.0 39.0 66-67 27.177144011052867 33.0 22.5 36.5 2.0 39.0 68-69 26.86187922083062 33.0 22.0 36.0 2.0 39.0 70-71 26.29754764568949 32.0 21.0 35.5 2.0 38.5 72-73 25.690744665157933 31.5 19.0 35.0 2.0 37.5 74-75 25.169335586841623 31.0 18.0 35.0 2.0 37.0 76-77 24.5559101523007 31.0 12.0 34.5 2.0 36.0 78-79 23.886420303881813 30.5 7.5 34.0 2.0 36.0 80-81 23.095572018119213 29.5 3.5 34.0 2.0 35.5 82-83 22.423594195806963 29.0 2.0 34.0 2.0 35.0 84-85 21.747895656209863 29.0 2.0 34.0 2.0 35.0 86-87 21.097392574576254 28.5 2.0 33.5 2.0 35.0 88-89 20.25275691973361 26.5 2.0 33.0 2.0 35.0 90-91 19.274670608807032 25.5 2.0 32.5 2.0 34.0 92-93 18.33253264228944 24.5 2.0 32.0 2.0 34.0 94-95 17.39026559975822 21.0 2.0 32.0 2.0 34.0 96-97 16.4667896861519 18.0 2.0 31.0 2.0 34.0 98-99 15.474549538204702 2.0 2.0 31.0 2.0 34.0 100-101 13.896700061524733 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.757748374955362 1101 2 7.948576526151623 1101 3 3.605027474394351 1101 4 2.1176272654172976 1101 5 1.6693353163109634 1101 6 1.3268320601008838 1101 7 0.8809535402930493 1101 8 0.9411815541607993 1101 9 0.9894691439541958 1101 10-11 0.8745446464617928 1101 12-13 0.5385920093069032 1101 14-15 0.574193343635784 1101 16-17 0.8631820007884627 1101 18-19 0.9433964987795136 1101 20-21 0.8060404258573186 1101 22-23 0.7754380702011616 1101 24-25 1.2158469253361304 1101 26-27 1.0883047918290103 1101 28-29 0.9975852479767511 1101 30-31 1.084586436207836 1101 32-33 1.0834564054459221 1101 34-35 1.1185332104534425 1101 36-37 0.9883860481688984 1101 38-39 0.9920810798055086 1101 40-41 1.0833825011612888 1101 42-43 0.8394752463257547 1101 44-45 0.6282816207590081 1101 46-47 0.7070822418480134 1101 48-49 0.8301187017467164 1101 50-51 0.6035823000970346 1101 52-53 0.7980221655723021 1101 54-55 0.9902390805174512 1101 56-57 0.6746676742007942 1101 58-59 0.7686623016064651 1101 60-61 0.9704672843190743 1101 62-63 0.9839686865086286 1101 64-65 0.8679999956563655 1101 66-67 0.6162279035754636 1101 68-69 0.5001103742784601 1101 70-71 0.36686526869586444 1101 72-73 0.5159488714386313 1101 74-75 0.4797285532974378 1101 76-77 0.31453761732327123 1101 78-79 0.14047829985932125 1101 80-81 0.06541910216819957 1101 82-83 0.3259812355586007 1101 84-85 0.15952983616548977 1101 86-87 -0.015238690588599724 1101 88-89 0.03700460631259617 1101 90-91 0.3900090557706619 1101 92-93 0.32479849970920327 1101 94-95 0.4055395882138093 1101 96-97 -0.561429655451068 1101 98-99 -0.4957765650245509 1101 100-101 -0.5229227728293608 1102 1 15.113628916827285 1102 2 8.122714457186103 1102 3 3.784799641882035 1102 4 2.280743028964096 1102 5 1.6945200453750004 1102 6 1.3129465921205892 1102 7 1.1482872841354137 1102 8 0.8406889433233644 1102 9 1.239407567599521 1102 10-11 1.3573956316834668 1102 12-13 1.0555870831985317 1102 14-15 1.1190825061973513 1102 16-17 1.124912296354971 1102 18-19 1.1100221145430638 1102 20-21 1.219833529305589 1102 22-23 1.2482521096100356 1102 24-25 1.211628945040566 1102 26-27 1.2895517130112708 1102 28-29 1.553404213493998 1102 30-31 1.4997034312817235 1102 32-33 1.5379052970715392 1102 34-35 1.4660085799115663 1102 36-37 1.4060892501393383 1102 38-39 1.4229924098547784 1102 40-41 1.7315352105208959 1102 42-43 1.5507283251434885 1102 44-45 1.41698235967527 1102 46-47 1.5979227590893856 1102 48-49 1.235291115539816 1102 50-51 1.1987485562546674 1102 52-53 1.4078435941437277 1102 54-55 1.418071592832721 1102 56-57 1.3742212456293679 1102 58-59 1.1906758484044957 1102 60-61 0.9174500429397625 1102 62-63 0.6065518145874442 1102 64-65 1.0171995030455285 1102 66-67 1.043383075989258 1102 68-69 0.864550029450875 1102 70-71 0.828687928794384 1102 72-73 1.0106533049361666 1102 74-75 1.0314680853171474 1102 76-77 1.2578873710178513 1102 78-79 1.0527473885292729 1102 80-81 1.0248248903455384 1102 82-83 0.9291062355585993 1102 84-85 0.7918266341950471 1102 86-87 0.8978924670468693 1102 88-89 0.9622663058199841 1102 90-91 0.8296180459184441 1102 92-93 1.4029542878865442 1102 94-95 1.091946607918242 1102 96-97 0.38749275834203445 1102 98-99 0.044617523645399615 1102 100-101 0.00646454244157546 1103 1 14.860265875884615 1103 2 7.967396994495072 1103 3 3.8891705483628805 1103 4 2.2521257786126867 1103 5 1.562918825169838 1103 6 1.2400251278743966 1103 7 0.992977298191704 1103 8 0.9503780601699674 1103 9 1.0956842969711715 1103 10-11 0.9294998874480385 1103 12-13 0.5176481981310914 1103 14-15 0.8784617118677147 1103 16-17 0.974728742208363 1103 18-19 0.9098737011088431 1103 20-21 0.969093331321325 1103 22-23 1.0620668956255273 1103 24-25 1.4667800501038144 1103 26-27 1.3736957749785503 1103 28-29 1.363538196805898 1103 30-31 1.2444341936108785 1103 32-33 1.0783242192236457 1103 34-35 1.1268995838892053 1103 36-37 1.4790772574366429 1103 38-39 1.5813544040528171 1103 40-41 1.4046484533357386 1103 42-43 1.352024419290693 1103 44-45 1.2381464144648753 1103 46-47 0.999067822940809 1103 48-49 0.8772023964561697 1103 50-51 0.8719791840023738 1103 52-53 1.006640211663246 1103 54-55 0.8851312871832953 1103 56-57 0.9826449229927654 1103 58-59 0.6450321752280743 1103 60-61 0.7418896607284395 1103 62-63 0.6924136592505441 1103 64-65 0.518918407253441 1103 66-67 0.6470713554401648 1103 68-69 0.6954323604016182 1103 70-71 0.4780441808504925 1103 72-73 0.44070571693485405 1103 74-75 0.5425557277491784 1103 76-77 0.6277624345702044 1103 78-79 0.36524327631824605 1103 80-81 0.07554602262415244 1103 82-83 0.3347762268855732 1103 84-85 0.15776967633399153 1103 86-87 -0.007657148648316792 1103 88-89 0.12778990243451105 1103 90-91 0.4107100996314017 1103 92-93 0.09842823889889729 1103 94-95 0.03644073119485469 1103 96-97 -0.9520573657719709 1103 98-99 -1.0003345504043057 1103 100-101 -0.9005583663122021 1104 1 14.883351090916118 1104 2 8.148409021964017 1104 3 3.9033030786058838 1104 4 2.2914936210047543 1104 5 1.7033775512954108 1104 6 1.2203008923574075 1104 7 1.027978845389967 1104 8 1.0477919872787709 1104 9 0.8917454062179004 1104 10-11 0.821486199464907 1104 12-13 0.8041250315610355 1104 14-15 1.0376415369834717 1104 16-17 1.1082810077279248 1104 18-19 1.1465401934433146 1104 20-21 1.1263921846512872 1104 22-23 0.9602445430415543 1104 24-25 1.1798110914045665 1104 26-27 1.165106209632306 1104 28-29 1.32064848080757 1104 30-31 1.245403916466266 1104 32-33 1.264274932605531 1104 34-35 1.471721772310346 1104 36-37 1.4924405468527908 1104 38-39 1.4691629175657468 1104 40-41 1.4547482585195084 1104 42-43 1.4410239122745523 1104 44-45 1.4922069019267532 1104 46-47 1.5638609269401371 1104 48-49 1.522895083656266 1104 50-51 1.6674028911475212 1104 52-53 1.7205948504251367 1104 54-55 1.584358487074045 1104 56-57 1.3455152154786099 1104 58-59 1.4966489610943796 1104 60-61 1.3729798471331414 1104 62-63 1.298887776003724 1104 64-65 1.342650031550118 1104 66-67 1.1653275685670863 1104 68-69 1.1495764546804708 1104 70-71 1.0198145388561883 1104 72-73 1.09950055844509 1104 74-75 1.0791280806963393 1104 76-77 1.0649174928915883 1104 78-79 0.5045942665977741 1104 80-81 0.45724056065109053 1104 82-83 0.4762377263458717 1104 84-85 0.7034443492068831 1104 86-87 0.5298446128332763 1104 88-89 0.5932957345729442 1104 90-91 0.4995784802741312 1104 92-93 0.6839461725974516 1104 94-95 0.5648398095586309 1104 96-97 -0.02611767890165595 1104 98-99 -0.19028182944187577 1104 100-101 -0.1892626567728879 1105 1 14.879210015167004 1105 2 8.158340835915933 1105 3 3.951164439281314 1105 4 2.34722683251686 1105 5 1.6734298329054837 1105 6 1.2265103910292154 1105 7 1.183884636974149 1105 8 1.2431416311208778 1105 9 1.0860841030691546 1105 10-11 1.1166171572843027 1105 12-13 0.8438649770798676 1105 14-15 0.9686137380561775 1105 16-17 1.3544127607692218 1105 18-19 1.2161538340368523 1105 20-21 0.9078922777091663 1105 22-23 0.8506815766946687 1105 24-25 1.2101500758892811 1105 26-27 1.071056415205625 1105 28-29 1.3872948439363526 1105 30-31 0.9633412462126429 1105 32-33 0.8321712322857522 1105 34-35 1.1999906419108797 1105 36-37 1.233849612382464 1105 38-39 1.21140527412971 1105 40-41 1.149550129828917 1105 42-43 1.2935081347336492 1105 44-45 1.3078000190274075 1105 46-47 1.3676356930264575 1105 48-49 1.565682674810688 1105 50-51 1.222368375133108 1105 52-53 1.1539294527295887 1105 54-55 1.0994021396805103 1105 56-57 1.1136572725653942 1105 58-59 0.9558346200287815 1105 60-61 0.897824186675976 1105 62-63 0.8089972459121881 1105 64-65 0.9276139927703646 1105 66-67 1.013323863171422 1105 68-69 0.9659563338744199 1105 70-71 0.8321971590277535 1105 72-73 0.7910390590288188 1105 74-75 0.7039123556062421 1105 76-77 0.6422612825469365 1105 78-79 0.7671993797053993 1105 80-81 0.6890287101528081 1105 82-83 0.42157256552493294 1105 84-85 0.539108359494012 1105 86-87 0.7307163959914895 1105 88-89 0.6437521411851286 1105 90-91 1.1873650562516644 1105 92-93 0.9782692188049218 1105 94-95 1.3718229576221823 1105 96-97 0.7768068765354634 1105 98-99 0.5545661478181625 1105 100-101 0.41697772019613133 1106 1 14.912920606569902 1106 2 8.035389712964808 1106 3 3.5748235437096483 1106 4 2.2750554141915984 1106 5 1.5663873027860262 1106 6 0.977576988249659 1106 7 0.7909228978585645 1106 8 0.891667606569932 1106 9 1.0901982225678921 1106 10-11 0.9778360665801316 1106 12-13 0.7180193127726753 1106 14-15 1.040592329265536 1106 16-17 1.074461586587283 1106 18-19 1.0042058817042872 1106 20-21 1.0122957090356834 1106 22-23 0.8458786872763966 1106 24-25 0.8663382609236194 1106 26-27 0.6272021925052513 1106 28-29 1.2180047323385494 1106 30-31 1.0893043144834067 1106 32-33 0.8456737554036593 1106 34-35 1.0355450447729666 1106 36-37 1.1104644505357584 1106 38-39 1.4133194568131202 1106 40-41 1.401165679521391 1106 42-43 1.0277756478473137 1106 44-45 0.9249585015705115 1106 46-47 1.215062482760942 1106 48-49 1.1795798175539858 1106 50-51 0.9952824268933149 1106 52-53 0.8091855214471977 1106 54-55 0.8640979139916709 1106 56-57 0.6391303749615709 1106 58-59 0.5533041021136498 1106 60-61 0.6640006740063065 1106 62-63 0.6287145656295117 1106 64-65 0.7623892602379385 1106 66-67 0.5899705071257593 1106 68-69 0.635269926264936 1106 70-71 0.5234375425758309 1106 72-73 0.34774303880972113 1106 74-75 0.16375486352567137 1106 76-77 0.19343660295302456 1106 78-79 0.573447974415533 1106 80-81 0.45688941070581635 1106 82-83 0.33431544942165914 1106 84-85 0.017893107509529216 1106 86-87 -0.006434273851485983 1106 88-89 0.28227352009112394 1106 90-91 0.2976194741983882 1106 92-93 0.8290171176297463 1106 94-95 0.4289784090084048 1106 96-97 0.24439769028012037 1106 98-99 -0.19779473915810897 1106 100-101 -0.36017710503561595 1107 1 14.767693668101522 1107 2 7.825455138868385 1107 3 3.68920893599633 1107 4 2.1141487352769133 1107 5 1.3025826325785133 1107 6 0.7824524151793355 1107 7 0.7003331852146033 1107 8 0.772498647472112 1107 9 0.5889840077031678 1107 10-11 0.7103569916310803 1107 12-13 0.5073884585224278 1107 14-15 0.7308820306712818 1107 16-17 0.970877087493669 1107 18-19 0.7416520726853832 1107 20-21 0.7061230022404743 1107 22-23 0.7653276242887017 1107 24-25 0.908791805600373 1107 26-27 0.9087656712674814 1107 28-29 0.9455466435176234 1107 30-31 0.8517261967363225 1107 32-33 0.9707525450000176 1107 34-35 0.9327569924517931 1107 36-37 1.028843314890615 1107 38-39 0.8628696842646377 1107 40-41 1.0876661510374177 1107 42-43 1.1124701885222876 1107 44-45 0.9898371533766799 1107 46-47 0.8340872996514719 1107 48-49 0.7122512368416771 1107 50-51 0.8670886997667289 1107 52-53 0.8068010673064059 1107 54-55 0.9222580730855761 1107 56-57 0.6399830127639667 1107 58-59 0.8066938870730205 1107 60-61 1.011053576639469 1107 62-63 0.7083054949314196 1107 64-65 0.6040136207595879 1107 66-67 0.5697270778116312 1107 68-69 0.42749992836599304 1107 70-71 0.3353417344266667 1107 72-73 0.6984117120662141 1107 74-75 0.656042033891989 1107 76-77 0.24623662640667376 1107 78-79 0.6665027631128275 1107 80-81 0.6216097503928104 1107 82-83 0.5599175485230958 1107 84-85 0.6871258312109063 1107 86-87 0.8669893234493848 1107 88-89 0.7749613569319145 1107 90-91 1.0828378129960967 1107 92-93 1.046229651649746 1107 94-95 1.253962895397958 1107 96-97 0.6060920208495091 1107 98-99 0.8047705035138488 1107 100-101 0.8652197746520596 1108 1 14.116595510769425 1108 2 7.383498465346364 1108 3 3.2113332321123913 1108 4 -0.6706885580361615 1108 5 0.15384200723713093 1108 6 0.24444929803961202 1108 7 0.38213252256796437 1108 8 0.2364069327263607 1108 9 0.2454689678771942 1108 10-11 0.3850087560990758 1108 12-13 0.02963115709420805 1108 14-15 0.3162111861055479 1108 16-17 0.39891316054899306 1108 18-19 0.22540583086074406 1108 20-21 0.24194839627638487 1108 22-23 -0.3983286571142486 1108 24-25 0.2683508870687277 1108 26-27 0.3296935258353457 1108 28-29 0.47683237205234974 1108 30-31 0.3159435497188099 1108 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2312 6 -0.8161000770963085 2312 7 -0.1519859548393896 2312 8 0.16544576063578376 2312 9 0.08938707467640938 2312 10-11 0.3695766251803789 2312 12-13 -0.6530001346822232 2312 14-15 0.20884355871388038 2312 16-17 -0.15169093332971784 2312 18-19 0.15780956194456053 2312 20-21 0.6027916144468577 2312 22-23 0.6657298406335883 2312 24-25 0.5301934840864106 2312 26-27 0.41137121134450894 2312 28-29 -0.16627172508188437 2312 30-31 0.348831115854658 2312 32-33 0.46330627526707246 2312 34-35 -0.1948752115524499 2312 36-37 0.3520562428711784 2312 38-39 0.44310553079034065 2312 40-41 0.665805525838671 2312 42-43 0.422391394163661 2312 44-45 0.3642520192195029 2312 46-47 0.5559184145896872 2312 48-49 0.4573175923964783 2312 50-51 0.6898102262912822 2312 52-53 0.43500286514214537 2312 54-55 0.33300000422132214 2312 56-57 0.4627117369130431 2312 58-59 0.26951553909797354 2312 60-61 0.5016083302778682 2312 62-63 0.2214071345502866 2312 64-65 0.17271756414176664 2312 66-67 0.12286419517609914 2312 68-69 0.3847280446429622 2312 70-71 0.253105929778048 2312 72-73 0.04096302131411278 2312 74-75 0.46626848424604717 2312 76-77 0.4581899831824501 2312 78-79 0.9029974021912182 2312 80-81 0.6249936154124818 2312 82-83 0.4852274716917222 2312 84-85 0.5758319077072578 2312 86-87 0.7038770836925892 2312 88-89 0.7680091059729968 2312 90-91 1.1709795957299107 2312 92-93 1.4944859289598256 2312 94-95 1.6885420502921455 2312 96-97 1.3581325474015458 2312 98-99 1.1786036704293767 2312 100-101 1.167641382706071 2313 1 -14.296715910758923 2313 2 -7.4322508405946 2313 3 -3.825036311263112 2313 4 -1.1561723140803757 2313 5 -0.5344364191135185 2313 6 -0.753014371311771 2313 7 -0.3648364642529174 2313 8 -0.1560993868552245 2313 9 -2.491453404062625 2313 10-11 -0.5106248330245151 2313 12-13 -1.0287835447333222 2313 14-15 -1.0538041560800977 2313 16-17 -0.7938649647758069 2313 18-19 -0.3394268334173276 2313 20-21 -1.2229969647552537 2313 22-23 -1.9158983032503585 2313 24-25 -1.0808877888359376 2313 26-27 -0.39799869436260593 2313 28-29 0.009513450045169236 2313 30-31 -1.2952088530295782 2313 32-33 -0.48169837066499355 2313 34-35 -1.2450052598272734 2313 36-37 -1.1398877613549203 2313 38-39 -0.632452958153344 2313 40-41 -0.10170245167426373 2313 42-43 0.057063537608861736 2313 44-45 -0.04952622672366047 2313 46-47 -0.5745928774837346 2313 48-49 0.07203397620972396 2313 50-51 -0.6183086818327332 2313 52-53 -0.06493882431291098 2313 54-55 -0.6790898204940277 2313 56-57 -0.007948603217091232 2313 58-59 0.10951140377716584 2313 60-61 -1.3131047124987454 2313 62-63 -0.20398058329475788 2313 64-65 -0.10909257168082931 2313 66-67 -1.0611288983338412 2313 68-69 -7.995302561454309E-4 2313 70-71 0.33054580057448746 2313 72-73 -0.491789223799465 2313 74-75 0.005890972322330157 2313 76-77 0.4451589947525214 2313 78-79 0.694939389187649 2313 80-81 0.617085129557676 2313 82-83 0.6014511042936839 2313 84-85 0.7059719034458638 2313 86-87 0.4057995665997183 2313 88-89 0.7361810041987162 2313 90-91 1.0594832147660043 2313 92-93 1.4726210505672555 2313 94-95 1.691882141344852 2313 96-97 1.217771816307085 2313 98-99 1.08996187116138 2313 100-101 0.706924724045285 2314 1 -14.296715910758923 2314 2 -7.308301993558757 2314 3 -3.5272554528553925 2314 4 -1.3429045700039204 2314 5 -1.1295110806549005 2314 6 -5.938115216074266 2314 7 -1.7963155954384717 2314 8 -4.602609389408904 2314 9 -1.3930285574594308 2314 10-11 -0.09982827044431275 2314 12-13 -2.7690637199196217 2314 14-15 -2.874634377315836 2314 16-17 -2.447675228265666 2314 18-19 -1.0826565411009526 2314 20-21 -1.7587222451000457 2314 22-23 -1.0165750604734711 2314 24-25 -2.7475947767670803 2314 26-27 -4.373533287127437 2314 28-29 -1.8629286297384553 2314 30-31 -1.8308320501730435 2314 32-33 -4.075076018988046 2314 34-35 -2.5908938668939925 2314 36-37 -0.9591422531757772 2314 38-39 -1.084257846754653 2314 40-41 -2.3172826522693555 2314 42-43 -1.1365185331248782 2314 44-45 -2.217206685182834 2314 46-47 -1.7269458152718613 2314 48-49 -0.8250200754053338 2314 50-51 -2.1102386687571233 2314 52-53 -0.7389484412545002 2314 54-55 -0.0067813769012339264 2314 56-57 -0.7736970970898618 2314 58-59 -1.246796410035877 2314 60-61 -0.46641928044359915 2314 62-63 -2.4142380569691326 2314 64-65 -0.6938104284328155 2314 66-67 -2.0191803828283987 2314 68-69 -1.8608962628896784 2314 70-71 -2.135712021270809 2314 72-73 -1.9868281082188801 2314 74-75 -1.595709973883345 2314 76-77 -0.549166267287692 2314 78-79 -0.21205481415056582 2314 80-81 -0.09429493932128352 2314 82-83 -0.2123961779570891 2314 84-85 0.2612113957563409 2314 86-87 0.33251982854713447 2314 88-89 0.4876148091628387 2314 90-91 0.7949750797908877 2314 92-93 1.2716864292575316 2314 94-95 1.3543137952015165 2314 96-97 1.2790118167934992 2314 98-99 1.320835031343682 2314 100-101 1.0968777529722704 2315 1 -14.296715910758923 2315 2 -7.458497428832301 2315 3 -4.605936581646073 2315 4 -2.885697263751286 2315 5 -3.3337480553013457 2315 6 -2.66459249203508 2315 7 -2.881560814323393 2315 8 -3.1051781151088385 2315 9 -2.0784741266016127 2315 10-11 -4.511683534521211 2315 12-13 -4.427996185456529 2315 14-15 -3.454286215390404 2315 16-17 -4.769026887590037 2315 18-19 -1.4548866890937049 2315 20-21 -3.080213593435282 2315 22-23 -4.146395301411147 2315 24-25 -4.855710837640444 2315 26-27 -4.686909091956018 2315 28-29 -2.7568962632772624 2315 30-31 -3.442494983176637 2315 32-33 -4.9052106248710245 2315 34-35 -4.415894901627404 2315 36-37 -5.446291834238089 2315 38-39 -3.8962170368688085 2315 40-41 -4.826097287538758 2315 42-43 -3.0800857895033644 2315 44-45 -1.9219451821808171 2315 46-47 -1.3954398642613164 2315 48-49 -1.1835067001825479 2315 50-51 -0.9421830880517952 2315 52-53 -0.8807014219334413 2315 54-55 -2.1978752970663784 2315 56-57 -4.270191937650377 2315 58-59 -3.2880011113471426 2315 60-61 -4.340143648156811 2315 62-63 -2.5481784770191602 2315 64-65 -3.065825222533803 2315 66-67 -1.9663685594470444 2315 68-69 -1.6733020051427623 2315 70-71 -2.6483965595080896 2315 72-73 -2.6675662870363333 2315 74-75 -3.249005082439812 2315 76-77 -2.869597660582105 2315 78-79 -2.039851569226581 2315 80-81 -1.682793748084439 2315 82-83 -1.2206927998111752 2315 84-85 -1.1307520885014846 2315 86-87 -1.531219685076426 2315 88-89 -2.1942324745326083 2315 90-91 -2.212174281390567 2315 92-93 -0.729342807135076 2315 94-95 -0.4088738821582609 2315 96-97 -1.4175590766729336 2315 98-99 -1.1153849710879395 2315 100-101 -0.7820652280429581 2316 1 -14.296715910758923 2316 2 -7.317128337821998 2316 3 -4.955631605808218 2316 4 -2.7963066057404014 2316 5 -1.4740091071962809 2316 6 -0.5620789927786305 2316 7 -4.641862529663374 2316 8 -2.7601816539418955 2316 9 -1.0757749380149448 2316 10-11 -4.575146938746073 2316 12-13 -3.2341423564921783 2316 14-15 -1.5637468283109897 2316 16-17 -3.0916620988464416 2316 18-19 -2.13448743715265 2316 20-21 -3.2096232180008855 2316 22-23 -2.923919337642431 2316 24-25 -3.2625235551726455 2316 26-27 -4.266620643926501 2316 28-29 -3.5431659886267113 2316 30-31 -2.705308010853763 2316 32-33 -2.810341071294154 2316 34-35 -4.172477860091842 2316 36-37 -2.363163386526665 2316 38-39 -3.370476333926675 2316 40-41 -2.9879651021834306 2316 42-43 -7.543418773813212 2316 44-45 -3.960948888014986 2316 46-47 -5.046650754854383 2316 48-49 -4.366470333702583 2316 50-51 -3.384215809666248 2316 52-53 -4.706202160184382 2316 54-55 -4.981533973304241 2316 56-57 -4.364226817025209 2316 58-59 -3.241223901432047 2316 60-61 -3.2414453290362424 2316 62-63 -2.255823814374658 2316 64-65 -2.408903490387683 2316 66-67 -2.6922531351714447 2316 68-69 -2.719938059357151 2316 70-71 -2.898789542280465 2316 72-73 -3.764941188624963 2316 74-75 -4.231346040389994 2316 76-77 -3.9902218338603355 2316 78-79 -4.341176908596694 2316 80-81 -3.82696143310444 2316 82-83 -3.5028908790333197 2316 84-85 -3.3928881652477756 2316 86-87 -3.71961648766786 2316 88-89 -3.205674038723327 2316 90-91 -2.593059413785337 2316 92-93 -2.7073645994546176 2316 94-95 -2.0227149607203536 2316 96-97 -2.1789224419308724 2316 98-99 -2.3051085047254105 2316 100-101 -1.865074389246745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7501.0 3 1825.0 4 1949.0 5 2384.0 6 3017.0 7 4089.0 8 5520.0 9 6829.0 10 8568.0 11 10873.0 12 13240.0 13 15144.0 14 16832.0 15 19333.0 16 22164.0 17 23007.0 18 18505.0 19 16735.0 20 17974.0 21 19523.0 22 21561.0 23 23613.0 24 26598.0 25 29531.0 26 33283.0 27 37006.0 28 41636.0 29 47266.0 30 53143.0 31 60963.0 32 70007.0 33 80759.0 34 93027.0 35 100536.0 36 97056.0 37 54087.0 38 6632.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.21335956018794 18.70065656779091 14.706888006976754 18.37909586504439 2 15.043536346907981 23.769711090065144 39.09367696678778 22.093075596239093 3 21.626416297302214 28.86040635192793 31.17718147156239 18.335995879207466 4 11.508534453236242 17.511490420143833 40.10580198626557 30.874173140354355 5 11.232175759573353 40.69023960173843 33.28515362572562 14.792431012962592 6 26.02065633309553 38.482103609543785 18.347943734641962 17.149296322718723 7 24.27048642045818 37.316827749847086 21.727987575770953 16.684698253923784 8 24.949780113465415 30.56720096422182 20.882825069046714 23.60019385326605 9 26.636030454868482 18.620595856905712 22.64569689425553 32.09767679397027 10-11 24.15316191161498 26.766950608372536 27.03145453292813 22.048432947084358 12-13 29.187170654576917 25.782176380756972 26.74281457849866 18.28783838616746 14-15 24.206872510498467 26.332237997697632 30.41776785730371 19.04312163450019 16-17 19.038879939255583 29.7824631122236 30.07902448646694 21.09963246205388 18-19 18.50823292241159 29.449086224205544 33.42788541872627 18.614795434656596 20-21 21.67748521835033 26.04588843976782 32.20259645240408 20.07402988947777 22-23 19.98285012378011 26.7564203891571 32.596976106428365 20.66375338063443 24-25 21.33225759924999 26.71352720585584 32.99981069484008 18.954404500054086 26-27 21.02479995889659 25.31487826269586 35.213453795169805 18.44686798323774 28-29 18.941099695880965 28.490518588757872 33.91724969669219 18.651132018668978 30-31 19.40052746947623 27.48601881346864 33.17454858358083 19.9389051334743 32-33 19.457933141932546 27.799236500160003 33.28733104073051 19.455499317176944 34-35 21.11874409650369 26.563029512073776 32.88842301840809 19.429803373014444 36-37 20.597889879939892 26.259662371778553 34.42577743933348 18.71667030894807 38-39 20.386139528387112 27.117403087764515 33.94013260310759 18.556324780740784 40-41 19.563222130054715 27.940708005671834 33.474212947797795 19.02185691647566 42-43 18.854164085397034 28.729880176525054 31.586179863618852 20.82977587445906 44-45 19.975981957177588 27.494800972437833 31.485806598427786 21.043410471956793 46-47 21.070257479294607 28.439100794410955 30.682246887148573 19.80839483914587 48-49 20.78127781947177 28.028369945166766 30.88028415019754 20.310068085163923 50-51 19.46025845673372 28.884593347851823 32.0967975329089 19.55835066250556 52-53 20.10540640717283 30.042133704167647 29.821815555020464 20.030644333639056 54-55 18.79825590071745 28.313119112639217 30.9477983023391 21.940826684304234 56-57 20.719367257552626 28.721321702223946 29.390502642962712 21.16880839726071 58-59 21.931529161044118 28.026096143925276 29.34901565002692 20.693359045003685 60-61 20.481255060856444 28.259687217104602 31.375530080356373 19.883527641682576 62-63 20.52525303504616 28.217175966210622 30.728893242890663 20.528677755852556 64-65 19.987672388312557 30.028232753959617 30.150562672568544 19.833532185159285 66-67 20.07748696384122 28.570141113151877 29.41079986950323 21.941572053503677 68-69 19.356712156444345 29.242076835356862 30.11588787067921 21.285323137519583 70-71 21.108171572331806 29.045331833672876 28.795012907984923 21.051483686010393 72-73 20.37256633219439 27.633873419752568 30.546995811547113 21.44656443650593 74-75 19.32346088229884 30.621490119133064 29.18340829495082 20.871640703617274 76-77 20.13765498697255 28.209488780252922 29.315390514646246 22.337465718128286 78-79 19.88698557880443 28.449626801747783 30.25994038205258 21.403447237395206 80-81 20.452424384785253 29.086125105162026 29.363130667816044 21.098319842236677 82-83 19.678581426895057 28.43606318373802 30.10577356981628 21.779581819550643 84-85 19.537650666889 28.589539340704906 29.599010313738656 22.273799678667437 86-87 19.773041858393878 28.985954052176844 29.297405130243792 21.943598959185486 88-89 19.804581050005 28.36639746004044 28.72297078631833 23.106050703636228 90-91 19.672878094991724 27.532790383560616 29.631113536773494 23.163217984674162 92-93 20.129541489646012 28.918118547768874 29.532115644532457 21.420224318052657 94-95 19.58528264645828 28.574236651459884 29.348281335870652 22.492199366211185 96-97 18.157531108280992 28.309392554216263 30.075231711144863 23.457844626357886 98-99 19.65054511552047 28.639369469290994 28.217818879386286 23.492266535802255 100-101 19.480268777969513 27.69501873128788 29.2850358072946 23.539676683448 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9803.0 1 6975.0 2 3798.0 3 3065.5 4 2663.5 5 2853.5 6 4062.5 7 8528.5 8 11085.0 9 8796.0 10 6490.5 11 5276.5 12 4904.5 13 4703.5 14 4406.5 15 4121.0 16 3995.0 17 4025.0 18 4120.5 19 4276.0 20 4585.0 21 4930.5 22 5329.0 23 5843.0 24 6545.5 25 7568.5 26 8760.5 27 9959.0 28 11213.5 29 12618.5 30 14071.0 31 15478.5 32 16859.0 33 18583.5 34 20401.0 35 21778.0 36 23403.5 37 24424.0 38 24612.0 39 24822.5 40 25338.0 41 26270.0 42 27069.0 43 28299.0 44 29721.5 45 32210.0 46 40462.5 47 65944.0 48 84465.0 49 69833.0 50 46140.5 51 32752.0 52 27221.0 53 24544.5 54 22850.0 55 20729.5 56 18594.5 57 16926.0 58 15751.0 59 14918.0 60 13577.5 61 11541.5 62 9125.5 63 6550.5 64 4556.5 65 3198.5 66 2216.5 67 1664.0 68 1192.0 69 823.5 70 610.0 71 494.5 72 382.5 73 281.0 74 217.0 75 157.5 76 105.5 77 62.0 78 36.0 79 22.0 80 11.5 81 7.0 82 5.5 83 5.0 84 6.0 85 4.0 86 3.5 87 4.0 88 2.5 89 1.0 90 0.5 91 1.0 92 1.0 93 1.5 94 1.5 95 1.0 96 2.0 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.17410060553291 2 0.08563091635874316 3 0.11531390207133271 4 0.1914102836254259 5 0.11891183973346477 6 0.14211853765421661 7 0.14922446453692742 8 0.14661595973188168 9 0.13627188895325199 10-11 0.12300449382414 12-13 0.13460784278451593 14-15 0.14265822830353642 16-17 0.13694650226490174 18-19 0.1440524291476126 20-21 0.17324069843165898 22-23 0.1906007476514462 24-25 0.21839481609141642 26-27 0.21146878609181216 28-29 0.20814069375434002 30-31 0.22392664524694444 32-33 0.2143471362215178 34-35 0.2011247153131825 36-37 0.1884869592749436 38-39 0.18398953719727854 40-41 0.18470912472970494 42-43 0.17904237291184694 44-45 0.19478335018367474 46-47 0.21906942940306617 48-49 0.22266736706519824 50-51 0.232741592519168 52-53 0.26058063517991487 54-55 0.2474931369339095 56-57 0.22320705771451801 58-59 0.18039159953514647 60-61 0.19145525784620257 62-63 0.19505319550833464 64-65 0.18385461453494856 66-67 0.19266956180717215 68-69 0.18093129018446627 70-71 0.1946034533005681 72-73 0.214841852650061 74-75 0.24609893608983333 76-77 0.24677354940148308 78-79 0.24160151401216823 80-81 0.24690847206381303 82-83 0.23687922083061988 84-85 0.20737613200113694 86-87 0.21807999654597982 88-89 0.21938424894850272 90-91 0.20818566797511667 92-93 0.20656659602715724 94-95 0.243175611739351 96-97 0.27344326232203703 98-99 0.28976890446396125 100-101 0.28558630193173273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1111748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.99385579048412 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55596528269608 56.64730144374121 2 9.450628845801425 12.662681320500202 3 2.9233858707359293 5.87546674332065 4 1.2225117381889405 3.276031003616158 5 0.6169353932172191 2.0665440382619993 6 0.37074733012364003 1.4902675901406088 7 0.20694296671874385 0.9704735088447334 8 0.14060303276131395 0.7535631440412943 9 0.10046809319346184 0.6057670452252931 >10 0.3718962138868531 4.271043692652487 >50 0.02029732631506593 0.916479114902576 >100 0.012176631693174081 1.6162234984598352 >500 0.003551517455316751 1.8705818068685085 >1k 0.003551517455316751 5.389224362538308 >5k 3.3823975764921436E-4 1.5883516868861067 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7250 0.6521262012614369 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6769 0.6088610008742988 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4697 0.4224878299758579 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4217 0.3793125780302731 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3665 0.32966103829285054 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3572 0.3212958332283935 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3097 0.2785703234905752 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3071 0.2762316640101894 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2997 0.26957547933524506 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2436 0.2191144036238428 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2267 0.20391311700133485 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2071 0.18628322245688772 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1830 0.16460564804254202 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1783 0.16037807128953682 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1770 0.15920874154934392 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1566 0.14085925947247038 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1504 0.13528245609616568 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1393 0.12529817908374918 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1197 0.10766828453930208 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1186 0.10667885168221575 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1180 0.10613916103289596 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7989688310660328E-4 0.0 12-13 0.0 0.0 0.0 3.597937662132066E-4 0.0 14-15 0.0 0.0 0.0 5.3969064931981E-4 0.0 16-17 0.0 0.0 0.0 0.0013492266232995247 0.0 18-19 0.0 0.0 0.0 0.0020688141557259378 0.0 20-21 0.0 0.0 0.0 0.0036878861036853676 0.0 22-23 0.0 0.0 0.0 0.008095359739797149 0.0 24-25 0.0 0.0 0.0 0.012907601362898785 0.0 26-27 0.0 0.0 0.0 0.01731507499901057 0.0 28-29 0.0 0.0 0.0 0.029098320842493083 0.0 30-31 0.0 0.0 0.0 0.0544188071397475 0.0 32-33 0.0 0.0 0.0 0.09993271856571813 0.0 34-35 0.0 0.0 0.0 0.16555010667885167 0.0 36-37 0.0 0.0 0.0 0.24456981258342717 0.0 38-39 0.0 0.0 0.0 0.3583995653691304 0.0 40-41 0.0 0.0 0.0 0.49098356821869704 0.0 42-43 0.0 0.0 0.0 0.615921953536233 0.0 44-45 0.0 0.0 0.0 0.7620431968395716 0.0 46-47 0.0 0.0 0.0 0.9399612142320022 0.0 48-49 0.0 0.0 0.0 1.1276386375329661 0.0 50-51 0.0 0.0 0.0 1.3279987910929454 0.0 52-53 0.0 0.0 0.0 1.5127978642642037 0.0 54-55 0.0 0.0 0.0 1.701059952435264 0.0 56-57 0.0 0.0 0.0 1.9408624976163662 0.0 58-59 0.0 0.0 0.0 2.188715428316489 0.0 60-61 0.0 0.0 0.0 2.4968338148573235 0.0 62-63 0.0 0.0 0.0 2.794203362632539 0.0 64-65 0.0 0.0 0.0 3.0607655691757483 0.0 66-67 0.0 0.0 0.0 3.3670400126647406 0.0 68-69 0.0 0.0 0.0 3.7204024653068863 0.0 70-71 0.0 0.0 0.0 4.112352799375397 0.0 72-73 0.0 0.0 0.0 4.515771559741956 0.0 74-75 0.0 0.0 0.0 4.946129878353728 0.0 76-77 0.0 0.0 0.0 5.342757531383011 0.0 78-79 0.0 0.0 0.0 5.855733493561491 0.0 80-81 0.0 0.0 0.0 6.39798767346557 0.0 82-83 0.0 0.0 0.0 6.918924072721516 0.0 84-85 0.0 0.0 0.0 7.5012502833375905 0.0 86-87 0.0 0.0 0.0 8.089737962200067 0.0 88-89 0.0 0.0 0.0 8.67597693002371 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7695 0.0 31.995468 1 TTTGCGT 400 0.0 28.343859 3 TTGCGTT 450 0.0 26.240765 4 TACATGG 9110 0.0 26.186697 2 ACATGGG 8965 0.0 25.556368 3 GTCCTAT 240 1.1351798E-4 25.225935 1 ATGGGAG 2200 0.0 24.884676 5 CTTTGCG 460 0.0 24.646833 2 GTATTAC 205 0.0013430523 24.610666 1 GAGTACT 8620 0.0 24.228262 12-13 CATGGGA 7185 0.0 23.79747 4 CATGGGG 1665 0.0 23.545767 4 AGTACTT 9030 0.0 23.128197 12-13 GCTTTGC 660 0.0 22.932669 1 GTCCAAG 400 3.0072988E-7 22.70334 1 GTACTTT 9610 0.0 21.88114 14-15 GTCGGGA 525 0.0 21.595322 2 CGGGAGT 505 0.0 21.51223 4 ACGCCGT 55 0.0029710282 21.473103 80-81 GTATAGG 285 3.6360198E-4 21.242891 1 >>END_MODULE