FastQCFastQC Report
Fri 27 May 2016
SRR939693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181983
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101820.3199891388483219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68920.2165944946908893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.15502911234912317No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32030.10066050007181057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149850.029.4868661
TACATGG103850.026.1415922
GGTATCA103300.025.2695831
CAACGCA178400.024.634575
ATCAACG176650.024.6107223
TCAACGC177850.024.5773264
TATCAAC180000.024.4427322
ACATGGG105450.024.3946843
GTACATG112100.024.3019961
AACGCAG184050.024.0031136
ACGCAGA199700.021.8603277
CGCAGAG199300.021.7144138
AGAGTAC145900.020.06847210-11
GAGTACT90550.020.01962512-13
CATGGGA63300.020.0187244
AGTACTT97750.019.7344412-13
GCAGAGT203300.019.4674789
CATGGGG51750.018.4337864
GTACTTT98950.018.20123914-15
GTATAGC15100.016.9727611
Produced by FastQC (version 0.11.5)