##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939693_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3181983 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.224668076479354 31.0 30.0 33.0 26.0 34.0 2 30.373004192668535 31.0 30.0 34.0 26.0 34.0 3 30.415706180705552 31.0 30.0 34.0 26.0 34.0 4 34.00613453937372 35.0 35.0 37.0 28.0 37.0 5 34.003175378372546 35.0 35.0 37.0 28.0 37.0 6 34.10753042992373 36.0 35.0 37.0 30.0 37.0 7 33.92107374552284 36.0 35.0 37.0 28.0 37.0 8 33.92769917375423 36.0 35.0 37.0 28.0 37.0 9 35.204665455472266 38.0 35.0 39.0 28.0 39.0 10-11 35.09240904178307 37.0 34.5 39.0 28.0 39.0 12-13 34.944021542541236 37.0 34.0 39.0 27.0 39.0 14-15 35.646811280889935 38.0 34.0 40.0 27.0 41.0 16-17 35.38485262177704 38.0 34.0 40.0 27.0 41.0 18-19 35.35692066865222 38.0 34.0 40.0 27.0 41.0 20-21 35.48059354811135 38.0 34.0 40.0 27.0 41.0 22-23 35.338553191516105 38.0 34.0 40.0 27.0 41.0 24-25 35.221164600816536 38.0 34.0 40.0 26.0 41.0 26-27 35.355693132238606 38.0 34.0 40.0 26.5 41.0 28-29 35.2412734448927 38.0 34.0 40.0 25.5 41.0 30-31 35.02200294596169 38.0 34.0 40.0 25.0 41.0 32-33 34.793485068902 38.0 34.0 40.0 24.5 41.0 34-35 34.56669551660081 38.0 33.0 40.0 24.0 41.0 36-37 34.29169231890931 38.0 33.0 40.0 24.0 41.0 38-39 34.14046492391694 38.0 33.0 40.0 23.5 41.0 40-41 33.87314451397132 38.0 33.0 40.0 22.0 41.0 42-43 33.56555141872222 38.0 32.0 40.0 20.0 41.0 44-45 33.313259687433906 37.5 31.5 40.0 18.5 41.0 46-47 32.884794796200985 37.0 31.0 40.0 16.0 41.0 48-49 32.52723804621206 37.0 31.0 40.0 14.5 41.0 50-51 31.932812494598494 36.5 30.0 39.0 13.0 40.5 52-53 31.997953006034287 36.5 30.5 39.0 10.5 40.0 54-55 32.04003069783842 37.0 30.0 39.0 9.0 40.0 56-57 31.91287099899654 36.5 30.0 39.0 8.0 40.0 58-59 31.521354136712862 36.0 30.0 39.0 7.5 40.0 60-61 31.114807181559424 35.5 29.0 39.0 6.5 40.0 62-63 30.699068473967333 35.0 28.5 38.5 2.0 40.0 64-65 30.096369308069843 34.5 28.0 38.0 2.0 40.0 66-67 29.556171890296085 34.0 27.5 38.0 2.0 40.0 68-69 29.03533535534288 34.0 26.0 37.5 2.0 39.0 70-71 28.483153901199344 34.0 26.0 36.5 2.0 39.0 72-73 27.74458034502384 33.0 25.5 36.0 2.0 39.0 74-75 27.28040611782024 32.0 25.0 36.0 2.0 38.0 76-77 26.895284952810872 32.0 24.0 35.0 2.0 38.0 78-79 26.305698050555264 32.0 22.5 35.0 2.0 37.0 80-81 25.626388638782796 31.0 20.5 35.0 2.0 36.5 82-83 24.953674013971792 31.0 18.5 34.0 2.0 36.0 84-85 24.27809953730111 31.0 15.0 34.0 2.0 35.5 86-87 23.59145900528067 30.0 7.5 34.0 2.0 35.0 88-89 22.781346097700713 29.5 2.0 34.0 2.0 35.0 90-91 21.501207423169767 28.5 2.0 33.5 2.0 35.0 92-93 19.81052051503732 26.0 2.0 33.0 2.0 34.0 94-95 19.570015144644078 26.0 2.0 32.0 2.0 34.0 96-97 18.942881058761156 25.5 2.0 32.0 2.0 34.0 98-99 17.939297758661816 24.5 2.0 32.0 2.0 34.0 100-101 16.29546669482521 18.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0988104752992705 1101 2 1.0558395881112261 1101 3 0.9379448454838482 1101 4 1.0662532361991595 1101 5 0.9109921591133201 1101 6 0.857198305446289 1101 7 0.8324657740859962 1101 8 0.9247024422640067 1101 9 1.2221337161755201 1101 10-11 0.946520500166379 1101 12-13 0.8628178223465568 1101 14-15 0.9010919852925312 1101 16-17 0.8096112331989431 1101 18-19 1.0305512662619591 1101 20-21 1.1051697542539145 1101 22-23 1.188712028689416 1101 24-25 1.0994062287948339 1101 26-27 1.1758396910196538 1101 28-29 1.158958640883725 1101 30-31 1.1758717708046689 1101 32-33 1.0317882450363385 1101 34-35 1.0877441625583373 1101 36-37 0.9199723725442581 1101 38-39 1.023921734981613 1101 40-41 1.1099807173783347 1101 42-43 1.2262658045740764 1101 44-45 0.9487704216452073 1101 46-47 1.0381585749665874 1101 48-49 1.0585565287408798 1101 50-51 1.1753769629206587 1101 52-53 1.181037372041338 1101 54-55 1.0860041222736143 1101 56-57 1.1230618030860775 1101 58-59 1.0939670381636546 1101 60-61 1.0214794955732636 1101 62-63 0.9745206043603467 1101 64-65 0.9740928267752196 1101 66-67 0.8315735903015664 1101 68-69 0.8227645525114831 1101 70-71 0.5043879455522102 1101 72-73 0.7288171858721562 1101 74-75 0.6245589200324382 1101 76-77 0.12212285775085618 1101 78-79 0.3666852286808684 1101 80-81 0.26988936625015114 1101 82-83 0.37402562506668247 1101 84-85 0.004860797788435178 1101 86-87 -0.3804945143240772 1101 88-89 -0.8886129885359608 1101 90-91 -4.1981745787844 1101 92-93 -9.287924315404732 1101 94-95 -7.603217079173756 1101 96-97 -4.189151890990317 1101 98-99 -3.0039074664813903 1101 100-101 -2.5509815080413034 1102 1 0.9485113076069425 1102 2 0.8413655992492828 1102 3 0.8311505095630096 1102 4 1.0095838073596397 1102 5 0.8722216103751848 1102 6 0.7864265624745954 1102 7 0.8424150800900705 1102 8 0.8055185424821403 1102 9 0.9082897464456892 1102 10-11 0.7635483818641333 1102 12-13 0.7345236265653767 1102 14-15 0.9337219800525318 1102 16-17 1.0014133681960757 1102 18-19 0.9526144265939038 1102 20-21 0.8609675753869723 1102 22-23 1.1001038591570023 1102 24-25 1.0971511989999243 1102 26-27 0.9651470355560292 1102 28-29 1.0128170510907637 1102 30-31 1.0484129292112172 1102 32-33 0.9650552659110616 1102 34-35 0.9098749555050532 1102 36-37 1.1498151114823614 1102 38-39 0.9712679164807412 1102 40-41 0.8273918505597564 1102 42-43 0.8111486965690631 1102 44-45 0.7121427909802165 1102 46-47 0.8132457052720952 1102 48-49 0.7055237238762047 1102 50-51 0.6849612965261471 1102 52-53 0.9103241221418727 1102 54-55 1.0601178912990932 1102 56-57 0.9021365157997927 1102 58-59 0.9164798639883429 1102 60-61 0.8410277584508776 1102 62-63 0.7614474027176605 1102 64-65 0.8004710697895625 1102 66-67 0.8764319883225511 1102 68-69 0.7089388618667201 1102 70-71 0.723431401030453 1102 72-73 0.728907667343254 1102 74-75 0.536059511056191 1102 76-77 0.2581753065389556 1102 78-79 0.34978865174420193 1102 80-81 0.17604941791902817 1102 82-83 0.04929615552573452 1102 84-85 -0.1489985263728606 1102 86-87 -0.6636634374422457 1102 88-89 -1.3140647865886308 1102 90-91 -5.345920158292326 1102 92-93 -10.714900589152311 1102 94-95 -9.440235577607847 1102 96-97 -5.809643050006169 1102 98-99 -4.498236684989575 1102 100-101 -3.771107694658575 1103 1 0.7937963978477747 1103 2 0.7983713493465174 1103 3 0.6906007758712001 1103 4 0.7373921414511884 1103 5 0.7668615519043698 1103 6 0.602880679178277 1103 7 0.7362286513593759 1103 8 0.677281375717321 1103 9 0.6403284163745084 1103 10-11 0.6220033544334456 1103 12-13 0.582394060907184 1103 14-15 0.5461481791392728 1103 16-17 0.7682173799662309 1103 18-19 0.631105203541928 1103 20-21 0.6919711578245327 1103 22-23 0.6478165682616677 1103 24-25 0.5473901614828094 1103 26-27 0.5167825787757394 1103 28-29 0.7121358080459288 1103 30-31 0.7335246166762843 1103 32-33 0.7344419721981694 1103 34-35 0.6538480017367903 1103 36-37 0.6881388946484108 1103 38-39 0.6980794070767189 1103 40-41 0.5613855635468141 1103 42-43 0.4385698914414604 1103 44-45 0.4056345127798906 1103 46-47 0.6162328862846778 1103 48-49 0.4860139369127552 1103 50-51 0.4045135768181396 1103 52-53 0.5216186988933273 1103 54-55 0.5457363492012135 1103 56-57 0.32718763277442164 1103 58-59 0.16802452147712188 1103 60-61 0.016914644209816032 1103 62-63 0.3106101341688259 1103 64-65 0.09168279954798919 1103 66-67 0.06327835344322352 1103 68-69 0.09296441985411619 1103 70-71 0.3182718610782267 1103 72-73 0.20328933942073846 1103 74-75 -0.004572359740098619 1103 76-77 -0.2012116197136251 1103 78-79 -0.14510944925394398 1103 80-81 -0.3026111323745724 1103 82-83 -0.2878802577773776 1103 84-85 -0.38750978010602566 1103 86-87 -0.8543550988514035 1103 88-89 -1.2621993294653997 1103 90-91 -3.142264832178526 1103 92-93 -9.588309763911873 1103 94-95 -6.047694056626643 1103 96-97 -3.5536278943856026 1103 98-99 -2.683065416878666 1103 100-101 -2.315723290010105 1104 1 0.923067062713752 1104 2 0.702692079700558 1104 3 0.7811643808086473 1104 4 0.8442692221098795 1104 5 0.7754494735706317 1104 6 0.7124806554654839 1104 7 0.7977720578133329 1104 8 0.8210796465359493 1104 9 0.8896978968975944 1104 10-11 0.7775615035758037 1104 12-13 0.7316306201067277 1104 14-15 0.9046617204412186 1104 16-17 1.0490391949953235 1104 18-19 0.9155616525881314 1104 20-21 0.9266880770880235 1104 22-23 0.974575900079607 1104 24-25 0.9683420819412163 1104 26-27 0.8573633199116912 1104 28-29 0.9612904840050476 1104 30-31 0.8713435583817173 1104 32-33 0.9524745328278925 1104 34-35 0.9006858636579338 1104 36-37 0.9657935696812459 1104 38-39 0.8210739623969943 1104 40-41 0.593743615614919 1104 42-43 0.7292219562144453 1104 44-45 0.6223846373646822 1104 46-47 0.3133617856961166 1104 48-49 0.5676936734043352 1104 50-51 0.42205446749289877 1104 52-53 0.4894717462651883 1104 54-55 0.6228134880303529 1104 56-57 0.6924424619404199 1104 58-59 0.6017821841366953 1104 60-61 0.6162332719059869 1104 62-63 0.42935250392908486 1104 64-65 0.5343984331334539 1104 66-67 0.4861452672416604 1104 68-69 0.36165908614286124 1104 70-71 0.5238222294601798 1104 72-73 0.5966246549954128 1104 74-75 0.4204314843260555 1104 76-77 0.4589051287436554 1104 78-79 0.2909993924687164 1104 80-81 0.022032110498155788 1104 82-83 0.01870884211831836 1104 84-85 -0.06003906447984875 1104 86-87 -0.05826946973061453 1104 88-89 -0.29752490797419995 1104 90-91 -0.7494130352860147 1104 92-93 -4.288520400211343 1104 94-95 -1.767638831753402 1104 96-97 -0.8550672424319821 1104 98-99 -0.778893354625172 1104 100-101 -0.898718530778325 1105 1 0.7689624908521715 1105 2 0.8189447809999884 1105 3 0.7369528795878004 1105 4 0.7437472047654339 1105 5 0.5711835253794533 1105 6 0.5179225459541001 1105 7 0.7543323564297069 1105 8 0.7413597397132392 1105 9 0.9527441206914844 1105 10-11 0.6672586773851279 1105 12-13 0.691985367377363 1105 14-15 0.8501866846577713 1105 16-17 0.8973721073469321 1105 18-19 0.7920947277193378 1105 20-21 0.7093478396543347 1105 22-23 0.7507637701927052 1105 24-25 0.7544538946885382 1105 26-27 0.6372431997915768 1105 28-29 0.7921063465966114 1105 30-31 0.7599265338805807 1105 32-33 0.8120490606267197 1105 34-35 0.7632255061041207 1105 36-37 0.7584628745000828 1105 38-39 0.7047974054662305 1105 40-41 0.5977064569609425 1105 42-43 0.7272279060895528 1105 44-45 0.6915701780455521 1105 46-47 0.7802726217658318 1105 48-49 0.5738792982815539 1105 50-51 0.5630622977530635 1105 52-53 0.6008228169620509 1105 54-55 0.7937208439844383 1105 56-57 0.7157830922903905 1105 58-59 0.6168449104143789 1105 60-61 0.3494571499896786 1105 62-63 0.5408523215036674 1105 64-65 0.7090441068445514 1105 66-67 0.678257472905269 1105 68-69 0.54256756471489 1105 70-71 0.4956739877605365 1105 72-73 0.6050246437692337 1105 74-75 0.3826960853757022 1105 76-77 0.20665427976383555 1105 78-79 -0.0444430593288736 1105 80-81 -0.018619288564458714 1105 82-83 -0.02310446651741671 1105 84-85 -0.12763750404090501 1105 86-87 -0.4749543789388859 1105 88-89 -0.3932659761451731 1105 90-91 -1.4254788206975952 1105 92-93 -7.586162902526743 1105 94-95 -3.2760949514244757 1105 96-97 -1.479508038368106 1105 98-99 -0.8962223658794208 1105 100-101 -0.47304425837507935 1106 1 0.8166849088105508 1106 2 0.8085388057530842 1106 3 0.7860624500659306 1106 4 0.7996581938692913 1106 5 0.709239297070468 1106 6 0.5702855136445208 1106 7 0.7384401346834721 1106 8 0.7145553486178216 1106 9 0.9310879305233684 1106 10-11 0.850862447176091 1106 12-13 0.8107722500434988 1106 14-15 0.7636774714588412 1106 16-17 0.9232253081430883 1106 18-19 0.8299833708452127 1106 20-21 0.8241261138151188 1106 22-23 0.8972574216385851 1106 24-25 0.7982584810344093 1106 26-27 0.7869313818932326 1106 28-29 0.92530417028253 1106 30-31 0.8479837626645761 1106 32-33 0.8796775543714261 1106 34-35 0.8327590032147043 1106 36-37 0.808865032163375 1106 38-39 0.756577098835578 1106 40-41 0.8706811705693127 1106 42-43 0.906030991629649 1106 44-45 0.8094520602599857 1106 46-47 0.7946303788917461 1106 48-49 0.6251156416775672 1106 50-51 0.5445095699292892 1106 52-53 0.6271113971415119 1106 54-55 0.7680046099286741 1106 56-57 0.7940199514973294 1106 58-59 0.6653104457940486 1106 60-61 0.5776816239062548 1106 62-63 0.6365008590194421 1106 64-65 0.5163061125621837 1106 66-67 0.32985736100052776 1106 68-69 0.3831344904526901 1106 70-71 0.3734161638997655 1106 72-73 0.6697344345598246 1106 74-75 0.6770431474391181 1106 76-77 0.5859067327798719 1106 78-79 0.29223823655133785 1106 80-81 0.4940883107582863 1106 82-83 0.6202558521779586 1106 84-85 0.41069190971230185 1106 86-87 0.30918530965120183 1106 88-89 0.1692510354154031 1106 90-91 -0.2309535561623619 1106 92-93 -3.923706943162152 1106 94-95 -1.4020784163333033 1106 96-97 -0.36642640327664466 1106 98-99 -0.3148394972130397 1106 100-101 -0.14161694837272165 1107 1 0.6621314282841411 1107 2 0.6608038058845729 1107 3 0.6592035645707242 1107 4 0.672791419005577 1107 5 0.6184792503353549 1107 6 0.5604843820836649 1107 7 0.47494471239562586 1107 8 0.47788434279847536 1107 9 0.6439744626199442 1107 10-11 0.3698287708430641 1107 12-13 0.502231492063018 1107 14-15 0.6127223413030407 1107 16-17 0.6189349944092015 1107 18-19 0.6181010896155215 1107 20-21 0.3955595087606625 1107 22-23 0.44362868780709164 1107 24-25 0.6267988821148762 1107 26-27 0.5570223162574379 1107 28-29 0.5041348343795136 1107 30-31 0.5652027790481711 1107 32-33 0.3346089262574736 1107 34-35 0.2953207093470027 1107 36-37 0.4466788224038467 1107 38-39 0.3559196514595513 1107 40-41 0.43613118390610595 1107 42-43 0.24521339946491594 1107 44-45 0.2086694161193421 1107 46-47 0.32696077625293185 1107 48-49 0.47265993484650437 1107 50-51 0.49768536947922826 1107 52-53 0.5612824123529592 1107 54-55 0.6843248487886981 1107 56-57 0.3747071061026581 1107 58-59 0.4590952200641887 1107 60-61 0.38833790666122425 1107 62-63 0.4975355966603736 1107 64-65 0.5183495529279512 1107 66-67 0.38986464927429765 1107 68-69 0.36447575823664025 1107 70-71 0.35786199211440106 1107 72-73 0.2587723448539485 1107 74-75 0.24948192030420202 1107 76-77 -0.007691889961797926 1107 78-79 0.05389421500209224 1107 80-81 0.06397510771223835 1107 82-83 0.19148191639741796 1107 84-85 -0.07959205120151225 1107 86-87 -0.13237987531691786 1107 88-89 -0.15346940668977993 1107 90-91 -0.46364925946199875 1107 92-93 -3.988230049619787 1107 94-95 -1.569098273122485 1107 96-97 -0.7247699270443064 1107 98-99 -0.5619788497588623 1107 100-101 -0.49594708463338577 1108 1 0.48251125266641637 1108 2 0.4100747522928643 1108 3 0.18589310596556174 1108 4 -0.1505833467837192 1108 5 -0.9765381072305033 1108 6 -0.13635337035337614 1108 7 0.2545655149636161 1108 8 0.21892316355877028 1108 9 0.2941123916778352 1108 10-11 0.36494473872888733 1108 12-13 0.3554030990977637 1108 14-15 0.3628799297298997 1108 16-17 0.4241422641661927 1108 18-19 0.26787444709540154 1108 20-21 0.37800969308404575 1108 22-23 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0.35538270083240775 2311 68-69 0.36052165677199 2311 70-71 0.5184627259934196 2311 72-73 0.4158715187217723 2311 74-75 0.5048665503068612 2311 76-77 0.6344580404164404 2311 78-79 0.41324495320008126 2311 80-81 0.40355296315465594 2311 82-83 0.6245810177065856 2311 84-85 0.6519712251329643 2311 86-87 0.8110252766934742 2311 88-89 0.8685350435747132 2311 90-91 1.7544868640397517 2311 92-93 2.6728979438568423 2311 94-95 2.0134113322620593 2311 96-97 1.7135111151053657 2311 98-99 1.5548744539060415 2311 100-101 1.4949984583645346 2312 1 0.2089798930549449 2312 2 0.038542546665354394 2312 3 0.16115681897764134 2312 4 0.06520376012112195 2312 5 0.1151786383359763 2312 6 0.13457771634691085 2312 7 0.17225392028362307 2312 8 -0.5178190205826922 2312 9 0.05555101484912228 2312 10-11 0.06197783017736924 2312 12-13 0.03620770080537028 2312 14-15 -0.17493763289185438 2312 16-17 0.017656944437966615 2312 18-19 0.09699822664409652 2312 20-21 0.1896650917292959 2312 22-23 0.08928192303454097 2312 24-25 0.13724014063666345 2312 26-27 0.15009896438395742 2312 28-29 0.1326246256720367 2312 30-31 0.17904032895837219 2312 32-33 0.3598327593612254 2312 34-35 0.23973185610479675 2312 36-37 0.4307029798149671 2312 38-39 0.4559231386354625 2312 40-41 0.5518958344039007 2312 42-43 0.511958600239069 2312 44-45 0.3531173961726992 2312 46-47 0.4747632015837837 2312 48-49 0.5000092016677655 2312 50-51 0.5113649067309751 2312 52-53 0.4996330653266554 2312 54-55 0.3271632792108292 2312 56-57 0.38926366809592494 2312 58-59 0.5444765427500116 2312 60-61 0.4789417111701404 2312 62-63 0.5352574682276803 2312 64-65 0.49352391172347865 2312 66-67 0.4676635438395351 2312 68-69 0.28260209861987917 2312 70-71 0.3454864407047076 2312 72-73 0.597310869429819 2312 74-75 0.7961643083222647 2312 76-77 0.8199836457556025 2312 78-79 1.0303235212284463 2312 80-81 0.9562913335417491 2312 82-83 1.1961089629337813 2312 84-85 1.077138634202914 2312 86-87 1.0135342442583415 2312 88-89 1.1539068421550915 2312 90-91 1.6161595561187063 2312 92-93 2.729410265620917 2312 94-95 2.1735527215944472 2312 96-97 1.848380344083619 2312 98-99 1.8038991339560262 2312 100-101 1.9046779916485903 2313 1 -0.33048821720808874 2313 2 -0.9951949332989933 2313 3 -0.4240445697379229 2313 4 -0.2358572358300819 2313 5 -0.7833098411372319 2313 6 -0.32966742655398207 2313 7 -1.1649991319674982 2313 8 -1.048281208033913 2313 9 -1.1433052026649904 2313 10-11 -2.131333170109869 2313 12-13 -0.565283598993986 2313 14-15 -0.3458286266729189 2313 16-17 -1.6323235328304193 2313 18-19 -1.1844592393941724 2313 20-21 -1.5171276766955728 2313 22-23 -1.1182691226689556 2313 24-25 -0.34337871514935614 2313 26-27 -0.9785172990878905 2313 28-29 -2.457421446282453 2313 30-31 -0.7563748791437988 2313 32-33 -0.2965591690996945 2313 34-35 -0.13633780134001228 2313 36-37 -0.0928457916912393 2313 38-39 -0.4626611904699871 2313 40-41 -0.3477978088847067 2313 42-43 -0.03433650139546529 2313 44-45 -0.591042938120772 2313 46-47 -0.8306173732348086 2313 48-49 -0.09497533878236197 2313 50-51 -0.21831700599754456 2313 52-53 -0.09228617359562463 2313 54-55 -0.04793519107845867 2313 56-57 -0.37469563494044067 2313 58-59 -0.08972364498971075 2313 60-61 -0.2020143823545304 2313 62-63 -0.16387976663437342 2313 64-65 -0.08825027520656903 2313 66-67 -0.06783601600007216 2313 68-69 -0.3211960139148111 2313 70-71 -0.17264351427244762 2313 72-73 0.16245160442364792 2313 74-75 0.18198606726327426 2313 76-77 0.20680899577935463 2313 78-79 0.21415683813420117 2313 80-81 -0.00365682017469382 2313 82-83 0.0051396751729484436 2313 84-85 0.2179600103037913 2313 86-87 0.25316682771854815 2313 88-89 0.5033213928721025 2313 90-91 1.1739294975420513 2313 92-93 2.257673089390508 2313 94-95 1.5353259863239543 2313 96-97 1.0083374659888023 2313 98-99 1.015751187607794 2313 100-101 1.1855606475523004 2314 1 -0.35914501949833166 2314 2 -0.504109527101047 2314 3 -1.463919664275398 2314 4 -0.30013622229556347 2314 5 -0.14319254507138623 2314 6 -2.6650163220314944 2314 7 -3.4513991913579147 2314 8 -1.3149914895351884 2314 9 -1.7963556891559875 2314 10-11 -0.9385045624902659 2314 12-13 -1.895665507189399 2314 14-15 -2.8844514186719223 2314 16-17 -2.1368135408574105 2314 18-19 -2.7082673712408862 2314 20-21 -2.1988725484726004 2314 22-23 -3.3461877206840143 2314 24-25 -1.91478455118542 2314 26-27 -1.903521590973213 2314 28-29 -1.384823162108603 2314 30-31 -1.3507402137020534 2314 32-33 -2.223791993438631 2314 34-35 -0.6562502932782266 2314 36-37 -0.3846471307594541 2314 38-39 -0.5687626274395967 2314 40-41 -0.669549547678244 2314 42-43 -0.6310194540269691 2314 44-45 -1.049703034722988 2314 46-47 -1.4427729204385926 2314 48-49 -1.607872802162138 2314 50-51 -0.7583285128900812 2314 52-53 -0.4787256719322315 2314 54-55 -0.12817906296220016 2314 56-57 -0.2566488371508804 2314 58-59 -0.404067821713312 2314 60-61 -0.43924428357157197 2314 62-63 -0.596034571457988 2314 64-65 -0.512770164035139 2314 66-67 -0.45648177538538093 2314 68-69 -0.36012828564451027 2314 70-71 -0.5815636759257252 2314 72-73 -0.407548349177592 2314 74-75 -0.11154269764812952 2314 76-77 -0.10859028875176335 2314 78-79 -0.03135152988220469 2314 80-81 -0.12872839026234217 2314 82-83 -0.1295420061325281 2314 84-85 -0.19976060420139063 2314 86-87 0.07437941291827244 2314 88-89 0.3241006136513249 2314 90-91 0.9773741415011621 2314 92-93 1.834275122584181 2314 94-95 1.282480118365548 2314 96-97 1.0770609896962462 2314 98-99 0.9140047382729186 2314 100-101 1.0735855915257986 2315 1 -7.070796886204402 2315 2 -2.8914265041631175 2315 3 -1.1960837140485872 2315 4 -0.7291728106055189 2315 5 -1.197368729924392 2315 6 -1.5164940561344835 2315 7 -2.8971795008358505 2315 8 -2.7502682029249996 2315 9 -2.9722842434613383 2315 10-11 -1.927028832438502 2315 12-13 -2.555866487314205 2315 14-15 -4.136597719485085 2315 16-17 -4.011845261098621 2315 18-19 -2.7259053051401807 2315 20-21 -3.9848182725079795 2315 22-23 -5.2533815035590194 2315 24-25 -3.121237638417881 2315 26-27 -4.237026748445558 2315 28-29 -2.5756620266656043 2315 30-31 -2.385361593884518 2315 32-33 -1.5963960635229242 2315 34-35 -2.2797734998954837 2315 36-37 -2.860627181571033 2315 38-39 -2.0426552517277656 2315 40-41 -3.3443553682209526 2315 42-43 -7.113192403647915 2315 44-45 -3.814492996202077 2315 46-47 -2.27551421306854 2315 48-49 -5.290700789033487 2315 50-51 -6.255600128210332 2315 52-53 -6.425343581808271 2315 54-55 -4.092907888194979 2315 56-57 -3.39041266098209 2315 58-59 -3.574929804734687 2315 60-61 -2.7448318658951614 2315 62-63 -2.964539468911024 2315 64-65 -1.6934383840529073 2315 66-67 -2.3651267196588037 2315 68-69 -1.7133383402316547 2315 70-71 -1.3483136333290666 2315 72-73 -1.8478938746681486 2315 74-75 -2.665025244010838 2315 76-77 -2.4690768673162786 2315 78-79 -1.4770140062215056 2315 80-81 -1.209987002080581 2315 82-83 -1.7154648569249922 2315 84-85 -1.3310024098938271 2315 86-87 -1.9825629545427006 2315 88-89 -1.3746984776763327 2315 90-91 -0.4853510975791906 2315 92-93 0.23137385677637923 2315 94-95 -0.02627745866041664 2315 96-97 -0.009981635289534552 2315 98-99 -0.09975362303891444 2315 100-101 -0.10695760782783381 2316 1 -4.194206129097047 2316 2 -2.786825181086737 2316 3 -4.553307020308868 2316 4 -4.104631589922864 2316 5 -3.9879333297011925 2316 6 -4.231723289539943 2316 7 -2.8773100526571866 2316 8 -1.4029377123766054 2316 9 -1.8201342533784484 2316 10-11 -2.7469714757660526 2316 12-13 -3.685818178877213 2316 14-15 -2.745293012343353 2316 16-17 -2.6703921043681547 2316 18-19 -2.739738592169573 2316 20-21 -5.220748076698314 2316 22-23 -6.169846049613614 2316 24-25 -4.120326673127053 2316 26-27 -3.547117671560663 2316 28-29 -4.070678723179945 2316 30-31 -6.007858516689652 2316 32-33 -4.052118191832648 2316 34-35 -5.9640446806965315 2316 36-37 -9.138603552471736 2316 38-39 -5.696400108467952 2316 40-41 -4.90035322475196 2316 42-43 -4.05250193783991 2316 44-45 -2.973822821635622 2316 46-47 -5.550239207405493 2316 48-49 -2.9509571599197386 2316 50-51 -4.018015349549792 2316 52-53 -5.031570751226138 2316 54-55 -6.675653721472614 2316 56-57 -4.671268968355474 2316 58-59 -5.609838715431181 2316 60-61 -5.950187341062101 2316 62-63 -6.07395709420172 2316 64-65 -4.971004657997131 2316 66-67 -6.314873291390953 2316 68-69 -5.134450155034198 2316 70-71 -6.038568860766915 2316 72-73 -7.824725270762652 2316 74-75 -7.507358883106729 2316 76-77 -6.7193556844739994 2316 78-79 -5.692054561608664 2316 80-81 -5.8433327302982505 2316 82-83 -4.725860573166262 2316 84-85 -6.160247719497381 2316 86-87 -4.669521103608634 2316 88-89 -4.553945169172511 2316 90-91 -5.20698784190521 2316 92-93 -3.918455637544854 2316 94-95 -3.849257194876081 2316 96-97 -4.7003974061345275 2316 98-99 -5.615686528810457 2316 100-101 -4.6694243475692225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15596.0 3 6144.0 4 6399.0 5 7426.0 6 8407.0 7 10060.0 8 11976.0 9 14620.0 10 17804.0 11 21346.0 12 25378.0 13 28666.0 14 31346.0 15 35027.0 16 39370.0 17 43447.0 18 41008.0 19 39831.0 20 42327.0 21 45840.0 22 51109.0 23 57263.0 24 64328.0 25 72669.0 26 82134.0 27 93759.0 28 106536.0 29 121169.0 30 139571.0 31 159553.0 32 184427.0 33 215128.0 34 253414.0 35 305191.0 36 356488.0 37 320506.0 38 104971.0 39 1749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63728723462813 19.405931874191047 13.626671573461161 23.33010931771966 2 14.965164225792691 20.687932616023826 41.23710724032972 23.10979591785376 3 18.873199651340958 25.714327955289605 30.101757230255576 25.310715163113862 4 12.182815209267094 16.947822268303792 38.96534780540715 31.904014717021965 5 13.049278449552807 38.149457527814064 35.16448431091358 13.636779711719543 6 30.52596618566402 37.60944589766 18.32944964663116 13.535138270044808 7 27.018960011474903 33.3019723746757 22.204219291424778 17.474848322424627 8 25.16594265145265 35.706945733615456 20.65410798295579 18.47300363197611 9 26.317885193866037 16.633924763446824 20.852283378184396 36.19590666450275 10-11 24.173177499041422 26.848356609185547 28.72232509611728 20.256140795655746 12-13 25.4182424561446 24.4034515942793 28.58921816360049 21.589087785975615 14-15 22.524955108564377 25.693220178238306 27.015765831298793 24.766058881898527 16-17 21.712731381910004 28.704512944962186 27.518025750735458 22.064729922392353 18-19 21.54173416586716 27.749264717447037 29.547663610198015 21.161337506487786 20-21 22.66577092151382 26.932409988072287 29.28492073017177 21.11689836024213 22-23 22.044186692583082 26.949119107179882 29.163188788905693 21.843505411331343 24-25 21.866742359898026 27.003523574465998 29.48862382971037 21.641110235925606 26-27 21.870595586463914 27.14518394664091 29.411494428675034 21.572726038220143 28-29 21.618688462886862 27.500838695424218 29.355032204016045 21.525440637672876 30-31 21.961448048303314 27.21199922769582 29.194372502068838 21.632180221932025 32-33 21.7857640756323 27.26459944921336 29.211895506000214 21.737740969154125 34-35 22.052099500157972 27.27101797341812 29.1590828131628 21.517799713261105 36-37 22.147884176989724 27.001620051886977 29.24420236676112 21.60629340436218 38-39 21.806451612903228 27.44335663235404 29.218592539855486 21.531599214887247 40-41 22.092392834903023 27.29584202979405 29.079863323575676 21.53190181172725 42-43 21.806278467996037 27.334389441477803 28.978494632114547 21.880837458411612 44-45 21.97042134093487 27.429054165663775 28.851164378497344 21.74936011490401 46-47 22.191366766109034 27.365233563687173 28.47614012562466 21.967259544579132 48-49 22.031652158312724 27.41426589669968 28.63439970980669 21.919682235180904 50-51 21.896837869081185 27.691069181598056 28.546606471575604 21.865486477745154 52-53 22.100342862431464 27.779427844874384 28.157972649390228 21.96225664330392 54-55 22.03468633873245 27.577496533412237 28.268405967724924 22.119411160130383 56-57 21.955495580917848 27.87336323078814 28.12090547086918 22.050235717424837 58-59 22.118084897661465 27.662155785794333 28.21829199928192 22.00146731726228 60-61 22.042129362895967 27.651422064234314 28.37762871925642 21.9288198536133 62-63 21.95164774585686 27.556323096603347 28.44738602086237 22.044643136677422 64-65 21.875100281070143 27.68440233740624 28.290886519266866 22.14961086225675 66-67 21.872765980476558 27.76755687624916 28.043285229543933 22.316391913730346 68-69 21.92820539928095 27.602546016724144 28.262050497141562 22.207198086853346 70-71 21.94674662995127 27.443357111176876 28.186695955910057 22.423200302961792 72-73 21.95992775829118 27.419823411954003 28.18440143123692 22.4358473985179 74-75 21.73230153403704 27.84981408473405 27.93786098599938 22.48002339522954 76-77 21.803982023896676 27.587803184618032 28.010919391963075 22.597295399522217 78-79 21.867187634034487 27.619985113269323 27.959729331597128 22.55309792109907 80-81 21.82571851234346 27.62025261462528 27.870326675080648 22.683702197950613 82-83 21.846979296987893 27.557276276694143 28.00840939842831 22.58733502788966 84-85 21.908616174430072 27.54861502246567 27.853602320169625 22.68916648293464 86-87 21.595895174226744 27.54524236195558 28.036108592684894 22.822753871132782 88-89 21.753195545706934 27.345254933762646 27.98666217959151 22.914887340938904 90-91 21.452667201278025 27.361850490524304 28.338850123438508 22.846632184759162 92-93 21.211481641156137 27.346693942070466 28.58716767975234 22.854656737021052 94-95 21.586875595186562 27.30372026034739 27.999905557173328 23.109498587292716 96-97 21.576391546514024 27.206664959139786 28.052205614153188 23.16473788019301 98-99 21.536797865945154 27.252341404688508 27.755120292509865 23.45574043685647 100-101 21.58232520172605 27.168834514411973 27.942199054843158 23.306641229018823 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3083.0 1 1860.5 2 670.5 3 714.0 4 652.5 5 633.5 6 912.0 7 1589.0 8 2407.0 9 2767.5 10 2508.0 11 2177.0 12 2237.5 13 2474.5 14 2697.0 15 2938.5 16 3136.0 17 3477.5 18 3906.5 19 4414.5 20 5239.5 21 6301.0 22 7699.0 23 9655.5 24 12083.5 25 15418.5 26 20744.0 27 27101.5 28 32359.0 29 37604.5 30 43267.5 31 49723.0 32 56503.5 33 63180.5 34 71328.5 35 79208.0 36 87331.0 37 93566.0 38 96223.0 39 98334.5 40 101471.0 41 104422.5 42 106429.5 43 109750.5 44 112798.0 45 114355.5 46 115332.0 47 117048.0 48 121707.5 49 120966.5 50 113834.5 51 108554.5 52 105045.5 53 101911.5 54 97037.0 55 89767.0 56 81774.0 57 73542.0 58 66196.5 59 59250.5 60 50347.5 61 40693.5 62 31714.0 63 23690.0 64 17252.5 65 12430.0 66 8610.5 67 6020.0 68 4305.5 69 3064.5 70 2130.0 71 1317.5 72 836.0 73 560.0 74 373.5 75 280.5 76 184.0 77 100.0 78 69.0 79 54.0 80 36.5 81 22.5 82 15.5 83 18.5 84 15.5 85 6.0 86 8.0 87 8.0 88 4.0 89 5.5 90 5.0 91 3.5 92 3.5 93 2.5 94 2.5 95 2.5 96 2.5 97 2.0 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08541843246805529 2 0.09638643575405652 3 0.09258377558899593 4 0.09676355907621129 5 0.09195523671873797 6 0.0768074499455214 7 0.09022675482552861 8 0.08554414024210688 9 0.08079867177165938 10-11 0.0878068801750355 12-13 0.08662836979330185 14-15 0.09190809630346862 16-17 0.08958250248351421 18-19 0.09217522532332825 20-21 0.1021847068321861 22-23 0.11187991890591496 24-25 0.13337594826873683 26-27 0.13882852296822454 28-29 0.13918993281862285 30-31 0.14074556652251127 32-33 0.13658149650705237 34-35 0.1313331969403985 36-37 0.13535584571004936 38-39 0.12870904715707154 40-41 0.12463925797215132 42-43 0.12452926366985617 44-45 0.11558829824043686 46-47 0.13397306019548189 48-49 0.1505507728985353 50-51 0.15997885595240452 52-53 0.16796129960468048 54-55 0.16430006068542793 56-57 0.13650292914827014 58-59 0.1280490813433007 60-61 0.12451355019809975 62-63 0.12157513097964383 64-65 0.10738586598357062 66-67 0.12115086724221971 68-69 0.1182753019107896 70-71 0.12647773416765581 72-73 0.15308064185132353 74-75 0.16552571148243092 76-77 0.17072687063381545 78-79 0.16745846850847412 80-81 0.17360243596524558 82-83 0.17401098623091324 84-85 0.15089646927717715 86-87 0.15224782784823176 88-89 0.17133969603231697 90-91 0.262037855010539 92-93 0.533708068207781 94-95 0.17152825769339433 96-97 0.1838319060786937 98-99 0.20213810067495644 100-101 0.20858062409510042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3181983.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.76115851806524 #Duplication Level Percentage of deduplicated Percentage of total 1 75.45788623538763 42.8307704204497 2 13.04439809140813 14.80830295678329 3 4.805731647094795 8.183366874480914 4 2.29633986680018 5.2137164476319136 5 1.2789785343908413 3.629815166588065 6 0.7891718910756542 2.6876586484407907 7 0.5025536459816 1.9967869014375264 8 0.35858208717026013 1.6282827753287858 9 0.25181655426789545 1.2864059418845692 >10 1.1266692374391665 10.834749568574226 >50 0.056652186948679316 2.205547338115735 >100 0.02914633746114442 3.0093195975418747 >500 0.0014571837550679263 0.5923093330498728 >1k 5.044097610162573E-4 0.5498083784142581 >5k 5.604552900180636E-5 0.21924893502465237 >10k+ 5.604552900180636E-5 0.323910716253774 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10182 0.3199891388483219 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6892 0.2165944946908893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4933 0.15502911234912317 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3203 0.10066050007181057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.1426943512897464E-5 2 0.0 0.0 0.0 0.0 3.1426943512897464E-5 3 0.0 0.0 0.0 0.0 3.1426943512897464E-5 4 0.0 0.0 0.0 0.0 3.1426943512897464E-5 5 0.0 0.0 0.0 0.0 3.1426943512897464E-5 6 0.0 0.0 0.0 0.0 3.1426943512897464E-5 7 0.0 0.0 0.0 0.0 3.1426943512897464E-5 8 0.0 0.0 0.0 0.0 3.1426943512897464E-5 9 0.0 0.0 0.0 0.0 3.1426943512897464E-5 10-11 0.0 3.1426943512897464E-5 0.0 1.5713471756448732E-5 3.1426943512897464E-5 12-13 0.0 3.1426943512897464E-5 0.0 3.1426943512897464E-5 3.1426943512897464E-5 14-15 0.0 3.1426943512897464E-5 0.0 6.285388702579493E-5 6.285388702579493E-5 16-17 0.0 3.1426943512897464E-5 0.0 1.4142124580803858E-4 9.428083053869238E-5 18-19 0.0 3.1426943512897464E-5 0.0 2.6712901985962844E-4 9.428083053869238E-5 20-21 0.0 3.1426943512897464E-5 0.0 4.556906809370132E-4 9.428083053869238E-5 22-23 0.0 3.1426943512897464E-5 0.0 6.44252342014398E-4 1.4142124580803858E-4 24-25 0.0 3.1426943512897464E-5 0.0 0.0010213756641691675 1.8856166107738477E-4 26-27 0.0 3.1426943512897464E-5 0.0 0.0014299259298368346 1.8856166107738477E-4 28-29 0.0 3.1426943512897464E-5 0.0 0.0035669580887138616 1.8856166107738477E-4 30-31 0.0 3.1426943512897464E-5 0.0 0.00966378513021597 1.8856166107738477E-4 32-33 0.0 3.1426943512897464E-5 0.0 0.021778871854437942 1.8856166107738477E-4 34-35 0.0 3.1426943512897464E-5 0.0 0.0395979488262508 2.1998860459028223E-4 36-37 0.0 3.1426943512897464E-5 0.0 0.06719080523057477 2.1998860459028223E-4 38-39 0.0 3.1426943512897464E-5 0.0 0.10732301209654482 2.1998860459028223E-4 40-41 0.0 3.1426943512897464E-5 0.0 0.1519178449413463 2.514155481031797E-4 42-43 0.0 3.1426943512897464E-5 0.0 0.20004820893134878 2.514155481031797E-4 44-45 0.0 3.1426943512897464E-5 0.0 0.25920943009437825 2.514155481031797E-4 46-47 0.0 3.1426943512897464E-5 0.0 0.327940155557085 2.8284249161607717E-4 48-49 0.0 3.1426943512897464E-5 0.0 0.4035848085926292 2.8284249161607717E-4 50-51 0.0 3.1426943512897464E-5 0.0 0.4839592166268645 2.8284249161607717E-4 52-53 0.0 3.1426943512897464E-5 0.0 0.5676491672017103 3.142694351289746E-4 54-55 0.0 3.1426943512897464E-5 0.0 0.6601072350166548 3.456963786418721E-4 56-57 0.0 3.1426943512897464E-5 0.0 0.765906040352824 3.456963786418721E-4 58-59 0.0 3.1426943512897464E-5 0.0 0.8847784541903587 3.456963786418721E-4 60-61 0.0 3.1426943512897464E-5 0.0 1.0158130951673847 3.456963786418721E-4 62-63 0.0 3.1426943512897464E-5 0.0 1.1508389579705485 3.7712332215476954E-4 64-65 0.0 3.1426943512897464E-5 0.0 1.2957643079802752 3.7712332215476954E-4 66-67 0.0 3.1426943512897464E-5 0.0 1.4606771940642047 3.7712332215476954E-4 68-69 0.0 3.1426943512897464E-5 0.0 1.6411307037152618 3.7712332215476954E-4 70-71 0.0 3.1426943512897464E-5 0.0 1.8470243241400095 4.08550265667667E-4 72-73 3.1426943512897464E-5 3.1426943512897464E-5 0.0 2.0609318151605462 4.714041526934619E-4 74-75 3.1426943512897464E-5 3.1426943512897464E-5 0.0 2.281156121827175 4.714041526934619E-4 76-77 3.1426943512897464E-5 3.1426943512897464E-5 0.0 2.5239921143513335 4.714041526934619E-4 78-79 3.1426943512897464E-5 3.1426943512897464E-5 0.0 2.8051061240742015 4.714041526934619E-4 80-81 3.1426943512897464E-5 3.1426943512897464E-5 0.0 3.1052962885093978 4.8711762444991064E-4 82-83 3.1426943512897464E-5 3.1426943512897464E-5 0.0 3.4038679653536805 5.028310962063594E-4 84-85 3.1426943512897464E-5 3.1426943512897464E-5 0.0 3.700978289324613 5.028310962063594E-4 86-87 3.1426943512897464E-5 3.1426943512897464E-5 0.0 4.025555761925817 5.185445679628082E-4 88-89 4.71404152693462E-5 3.1426943512897464E-5 0.0 4.381450183737625 5.342580397192569E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14985 0.0 29.486866 1 TACATGG 10385 0.0 26.141592 2 GGTATCA 10330 0.0 25.269583 1 CAACGCA 17840 0.0 24.63457 5 ATCAACG 17665 0.0 24.610722 3 TCAACGC 17785 0.0 24.577326 4 TATCAAC 18000 0.0 24.442732 2 ACATGGG 10545 0.0 24.394684 3 GTACATG 11210 0.0 24.301996 1 AACGCAG 18405 0.0 24.003113 6 ACGCAGA 19970 0.0 21.860327 7 CGCAGAG 19930 0.0 21.714413 8 AGAGTAC 14590 0.0 20.068472 10-11 GAGTACT 9055 0.0 20.019625 12-13 CATGGGA 6330 0.0 20.018724 4 AGTACTT 9775 0.0 19.73444 12-13 GCAGAGT 20330 0.0 19.467478 9 CATGGGG 5175 0.0 18.433786 4 GTACTTT 9895 0.0 18.201239 14-15 GTATAGC 1510 0.0 16.972761 1 >>END_MODULE