FastQCFastQC Report
Fri 27 May 2016
SRR939693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181983
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104690.32900867163652353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79930.2511955594985894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57110.17947927440215738No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34830.10946004425542186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140550.028.47321
GGTATCA98200.028.4309521
TACATGG103650.024.3415372
ATCAACG164100.024.1212023
TCAACGC165600.023.8739154
GTACATG110000.023.626241
CAACGCA168800.023.4213285
ACATGGG103100.023.2364943
AACGCAG171200.023.1145116
TATCAAC172550.023.0239582
GAGTACT92100.020.61537712-13
CGCAGAG188150.020.3577488
ACGCAGA191100.020.3386067
CATGGGG49200.019.8977034
GCAGAGT197750.018.7750079
AGTACTT97650.018.62551312-13
AGAGTAC148000.018.57649210-11
CATGGGA60250.018.5138174
GTACTTT102550.018.37715314-15
AGTACAT78450.017.4003332
Produced by FastQC (version 0.11.5)