##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939693_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3181983 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55206988849406 31.0 31.0 34.0 30.0 34.0 2 31.76319452366653 31.0 31.0 34.0 30.0 34.0 3 31.81009609416518 31.0 31.0 34.0 30.0 34.0 4 35.59857170827122 37.0 35.0 37.0 33.0 37.0 5 35.330831748629706 37.0 35.0 37.0 33.0 37.0 6 35.3536068545935 37.0 35.0 37.0 33.0 37.0 7 35.281939595528954 37.0 35.0 37.0 32.0 37.0 8 35.24748938005011 37.0 35.0 37.0 32.0 37.0 9 36.86073307116977 39.0 37.0 39.0 33.0 39.0 10-11 36.74381635602704 39.0 36.0 39.0 32.0 39.0 12-13 36.68873608061388 39.0 35.0 39.0 32.0 39.0 14-15 37.75652289782818 40.0 37.0 41.0 32.0 41.0 16-17 37.72529771529263 39.0 37.0 41.0 32.0 41.0 18-19 37.70835152167689 39.5 37.0 41.0 32.0 41.0 20-21 37.64253674516803 39.0 36.5 41.0 32.0 41.0 22-23 37.5076776023002 39.0 36.0 41.0 32.0 41.0 24-25 37.38513514999923 39.0 36.0 41.0 32.0 41.0 26-27 37.13294665622035 39.0 36.0 40.5 31.0 41.0 28-29 36.94189613835147 39.0 36.0 40.0 31.0 41.0 30-31 36.70948462012525 38.5 35.5 40.0 30.0 41.0 32-33 36.542336964088115 38.0 35.0 40.0 30.0 41.0 34-35 36.52698018814054 38.0 35.0 40.0 30.0 41.0 36-37 36.43509613344886 38.0 35.0 40.0 30.0 41.0 38-39 36.47341233438394 39.0 35.0 40.0 30.0 41.0 40-41 36.39013831940649 39.0 35.0 40.0 30.0 41.0 42-43 36.20951337577856 38.5 35.0 40.0 30.0 41.0 44-45 36.007085675819134 38.0 35.0 40.0 29.0 41.0 46-47 35.72624225207991 38.0 35.0 40.0 28.5 41.0 48-49 35.43985087286764 38.0 34.0 40.0 27.0 41.0 50-51 35.20915903699046 38.0 34.0 40.0 27.0 41.0 52-53 34.98242102487663 38.0 34.0 40.0 26.0 41.0 54-55 34.705149273267644 38.0 34.0 40.0 26.0 41.0 56-57 32.64042501169868 37.0 30.5 39.5 16.5 41.0 58-59 27.55331722388209 32.5 17.5 38.5 7.0 40.0 60-61 28.340744278017826 33.0 20.0 38.0 9.0 40.0 62-63 31.494237084233323 35.0 29.5 38.5 13.5 40.0 64-65 31.744949925879553 36.0 31.0 39.0 10.0 40.0 66-67 24.956214410950654 27.5 18.0 35.5 2.0 39.0 68-69 17.42030661383169 13.0 7.5 28.0 2.0 35.5 70-71 16.282595947244218 12.5 7.5 26.5 2.0 33.5 72-73 17.407882285983302 13.5 7.0 28.5 2.0 34.0 74-75 18.431808089483823 17.5 8.5 29.5 2.0 34.5 76-77 19.659321404294115 21.5 8.0 30.0 2.0 34.5 78-79 22.767904008286656 27.5 10.5 33.5 2.0 36.0 80-81 26.452968007685772 31.5 23.5 34.5 2.0 36.0 82-83 27.78722780731387 33.5 27.0 35.0 2.0 36.0 84-85 27.841077875023217 34.0 27.5 35.0 2.0 36.0 86-87 27.59601889764967 34.0 27.0 35.0 2.0 36.0 88-89 27.16735145976581 33.5 26.0 35.0 2.0 35.5 90-91 25.778927637262676 32.5 21.0 35.0 2.0 35.0 92-93 23.23408673145017 31.5 2.0 34.5 2.0 35.0 94-95 15.837627039490783 4.5 2.0 32.0 2.0 35.0 96-97 13.262511144779843 2.0 2.0 30.0 2.0 34.0 98-99 12.91689867607715 2.0 2.0 29.5 2.0 34.0 100-101 12.627405457540156 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3825622571179146 1101 2 0.3272576257008737 1101 3 0.35101768551531976 1101 4 0.23463239603613317 1101 5 0.3507115147903832 1101 6 0.4441757820146819 1101 7 0.3344805787353238 1101 8 0.29632211555085064 1101 9 0.37041790238965433 1101 10-11 0.3613334359202227 1101 12-13 0.4394429787502432 1101 14-15 0.5662460598638788 1101 16-17 0.5360811051000027 1101 18-19 0.38114190875707976 1101 20-21 0.4202285840196396 1101 22-23 0.5287189125554761 1101 24-25 0.5350702324551477 1101 26-27 0.5016319379116254 1101 28-29 0.6397813590439299 1101 30-31 0.6259841122159315 1101 32-33 0.6643105854876339 1101 34-35 0.44864333481680774 1101 36-37 0.24268810088576487 1101 38-39 0.38017562494682977 1101 40-41 0.3735905664284189 1101 42-43 0.47560528287457515 1101 44-45 0.2509523761746806 1101 46-47 0.38516795538468784 1101 48-49 0.3348571162818388 1101 50-51 0.20129434130556945 1101 52-53 0.06421545617103419 1101 54-55 -0.10734903984074151 1101 56-57 -2.318331054428029 1101 58-59 -10.122243691084837 1101 60-61 -10.175863989281286 1101 62-63 -3.8561256050417327 1101 64-65 -4.885752374541095 1101 66-67 -9.718898334509358 1101 68-69 -4.824873944773376 1101 70-71 -4.415976927284365 1101 72-73 -4.597517073554133 1101 74-75 -6.595063961821182 1101 76-77 -8.045314076357652 1101 78-79 -11.162655737499492 1101 80-81 -7.910007243902587 1101 82-83 -4.072107785544677 1101 84-85 -3.2152154316153876 1101 86-87 -2.9420140877703176 1101 88-89 -3.1295750226164003 1101 90-91 -9.741121323953344 1101 92-93 -17.310422685308872 1101 94-95 -11.25904379577311 1101 96-97 -9.25850878390451 1101 98-99 -9.323290440320534 1101 100-101 -9.300461576278297 1102 1 0.3804875809623738 1102 2 0.3302968282453236 1102 3 0.3443202287453566 1102 4 0.2363372253704057 1102 5 0.2674216450593292 1102 6 0.3057909219715427 1102 7 0.3630745515116729 1102 8 0.4544057362720224 1102 9 0.4903354027856963 1102 10-11 0.46813477882367494 1102 12-13 0.3712418844423482 1102 14-15 0.34999991370431616 1102 16-17 0.3238298529826835 1102 18-19 0.4269170028943634 1102 20-21 0.5121269888648143 1102 22-23 0.5606576532483771 1102 24-25 0.434986453315247 1102 26-27 0.39832007234820566 1102 28-29 0.3988782503808679 1102 30-31 0.3181082637617294 1102 32-33 0.3575579868110381 1102 34-35 0.15472752828264902 1102 36-37 0.25255088588716745 1102 38-39 0.2379360105136641 1102 40-41 0.3108354208659776 1102 42-43 0.31726148984255786 1102 44-45 0.35296277088104944 1102 46-47 0.31604620449206067 1102 48-49 0.30614615736103445 1102 50-51 0.3680861181545012 1102 52-53 0.17804876310124484 1102 54-55 0.09413369860992304 1102 56-57 -2.8534719861739255 1102 58-59 -10.36230839904599 1102 60-61 -10.693946269536216 1102 62-63 -4.542993605675409 1102 64-65 -5.41217692457095 1102 66-67 -6.870872171045594 1102 68-69 -4.404961318800014 1102 70-71 -4.342669570561927 1102 72-73 -4.854654751622888 1102 74-75 -5.467353782808008 1102 76-77 -7.7493373029817505 1102 78-79 -10.481661720853339 1102 80-81 -6.130753700806601 1102 82-83 -3.5582467553354427 1102 84-85 -3.11717281697506 1102 86-87 -3.0158207681666056 1102 88-89 -3.090301494387397 1102 90-91 -8.90206227032251 1102 92-93 -13.248206772756014 1102 94-95 -10.274173089834845 1102 96-97 -9.057162833940458 1102 98-99 -9.240182142834518 1102 100-101 -9.076768835512281 1103 1 0.11703624282246139 1103 2 0.12205662195771083 1103 3 0.1658247525351193 1103 4 0.1490628274508765 1103 5 0.22719846009936617 1103 6 0.1553955340474431 1103 7 0.14686989564264508 1103 8 0.13557849214934947 1103 9 0.23836236255301202 1103 10-11 0.28259235898358526 1103 12-13 0.2186732877120363 1103 14-15 0.34813380668311567 1103 16-17 0.2509359473258641 1103 18-19 0.19993263943943163 1103 20-21 0.16477467317837835 1103 22-23 0.31138868847584433 1103 24-25 0.322949593102301 1103 26-27 0.3627029142420284 1103 28-29 0.26939502924658143 1103 30-31 0.2561761870977719 1103 32-33 0.3045369611120705 1103 34-35 0.27743891259531495 1103 36-37 0.10542993387662847 1103 38-39 0.24940271024180305 1103 40-41 0.1912757417356019 1103 42-43 0.24901165506661727 1103 44-45 0.19089511550294702 1103 46-47 0.15089388340646792 1103 48-49 0.1170313983781952 1103 50-51 0.2711423883200865 1103 52-53 0.16471535644608792 1103 54-55 0.18456315118586986 1103 56-57 -2.699953138632992 1103 58-59 -10.166557281971368 1103 60-61 -10.382181216649517 1103 62-63 -4.095006389668324 1103 64-65 -5.270686138624345 1103 66-67 -6.263313157810671 1103 68-69 -3.8570570397718207 1103 70-71 -4.186242079677996 1103 72-73 -4.643986926471438 1103 74-75 -5.0611420580487945 1103 76-77 -6.843030143205834 1103 78-79 -9.573126160936244 1103 80-81 -5.213636188256071 1103 82-83 -2.864994845885228 1103 84-85 -2.341039669879155 1103 86-87 -2.155647415382994 1103 88-89 -2.2494299112259526 1103 90-91 -5.163349898164938 1103 92-93 -10.474058564024784 1103 94-95 -8.759180743633198 1103 96-97 -8.322354548841219 1103 98-99 -8.505932270064543 1103 100-101 -8.64510750634096 1104 1 0.23724287758800955 1104 2 0.32141193038149396 1104 3 0.2636844876558051 1104 4 0.2590583257252206 1104 5 0.2784373609372821 1104 6 0.22735219150688124 1104 7 0.2813714545735664 1104 8 0.3055614300533236 1104 9 0.24775651920194974 1104 10-11 0.2846388842256715 1104 12-13 0.34097044606718185 1104 14-15 0.43710281887852886 1104 16-17 0.40751532127107026 1104 18-19 0.33588123454903496 1104 20-21 0.30656096903623364 1104 22-23 0.41456472076970385 1104 24-25 0.425143952792034 1104 26-27 0.4889995687003079 1104 28-29 0.5434752785273176 1104 30-31 0.49679740671343353 1104 32-33 0.483471663859234 1104 34-35 0.5625954448215538 1104 36-37 0.4995532366982687 1104 38-39 0.5072382138515223 1104 40-41 0.39548652253490246 1104 42-43 0.5039351164675665 1104 44-45 0.5386474020275926 1104 46-47 0.5252095006893924 1104 48-49 0.5166471770192658 1104 50-51 0.4673287968137174 1104 52-53 0.40703139240802244 1104 54-55 0.313949138036385 1104 56-57 -2.6470853726560293 1104 58-59 -10.160257644888265 1104 60-61 -10.256259798624463 1104 62-63 -4.044056581130604 1104 64-65 -5.4725273073686616 1104 66-67 -6.7167855073965335 1104 68-69 -3.926076274923073 1104 70-71 -4.014395834913799 1104 72-73 -4.315373087462781 1104 74-75 -5.213910063562018 1104 76-77 -6.7703349619048545 1104 78-79 -9.35403847361907 1104 80-81 -4.913866554340846 1104 82-83 -2.571985859106963 1104 84-85 -1.9826740556529607 1104 86-87 -1.92503808921537 1104 88-89 -1.9102152493092532 1104 90-91 -4.56389955331052 1104 92-93 -6.665618106293763 1104 94-95 -7.903641212738948 1104 96-97 -7.956862776995344 1104 98-99 -8.45391881305417 1104 100-101 -8.480096474334246 1105 1 0.2476748048423687 1105 2 0.23651675483210965 1105 3 0.2562667895839006 1105 4 0.08547913906191695 1105 5 0.1841935844505116 1105 6 0.17362089011202642 1105 7 0.25212164401534665 1105 8 0.19674607263984 1105 9 0.31142579595534414 1105 10-11 0.40137571451390386 1105 12-13 0.26748387538435026 1105 14-15 0.42141142655162867 1105 16-17 0.30644041458868543 1105 18-19 0.3979690232723385 1105 20-21 0.3148711483744506 1105 22-23 0.3561549090888647 1105 24-25 0.4652115905232179 1105 26-27 0.3865270026763383 1105 28-29 0.44401273186851853 1105 30-31 0.4211273441274912 1105 32-33 0.41258255044675707 1105 34-35 0.44830311510866494 1105 36-37 0.3441520411101564 1105 38-39 0.32823678251364186 1105 40-41 0.22514468537063692 1105 42-43 0.14857040596266557 1105 44-45 0.14049691509205786 1105 46-47 0.21903650526406437 1105 48-49 0.02510610323520268 1105 50-51 -0.028169505226642855 1105 52-53 0.16132680932026489 1105 54-55 0.059558298598815895 1105 56-57 1.202632569585269 1105 58-59 -8.895455927282807 1105 60-61 -10.140345285980978 1105 62-63 -4.0397288889524425 1105 64-65 -5.5464713448640985 1105 66-67 -6.9160389664630735 1105 68-69 -4.0667473197997275 1105 70-71 -4.246380623440643 1105 72-73 -2.401226698082745 1105 74-75 -4.670321615300676 1105 76-77 -6.5942705797657055 1105 78-79 -9.35128384983641 1105 80-81 -4.985058774598489 1105 82-83 -2.5331166160321104 1105 84-85 -2.0911792898591273 1105 86-87 -1.992696869490974 1105 88-89 -2.09663578199147 1105 90-91 -4.624584069602516 1105 92-93 -6.968080849410715 1105 94-95 -8.44667115857263 1105 96-97 -8.097470674612588 1105 98-99 -8.57988636939556 1105 100-101 -8.561602746962015 1106 1 0.0822221880490801 1106 2 0.13174827233617137 1106 3 0.11726070184941761 1106 4 0.03404463102022248 1106 5 0.05371469627074532 1106 6 0.0902413669311315 1106 7 0.1824661882220937 1106 8 0.172218826458149 1106 9 0.11234580446476627 1106 10-11 0.17154435845575478 1106 12-13 0.12557001289312808 1106 14-15 0.2359011963017963 1106 16-17 0.25456499021034773 1106 18-19 0.20084751046011462 1106 20-21 0.282883877538751 1106 22-23 0.29118371581714086 1106 24-25 0.30153779284349724 1106 26-27 0.37204903537934086 1106 28-29 0.3704805344848765 1106 30-31 0.36107136261508543 1106 32-33 0.1623131339542141 1106 34-35 0.2878564869675415 1106 36-37 0.2965492504856755 1106 38-39 0.30092095178122236 1106 40-41 0.37305754538189007 1106 42-43 0.20534180847294436 1106 44-45 0.32430700924940936 1106 46-47 0.30335162784928826 1106 48-49 0.3448454875842728 1106 50-51 0.21023859154763613 1106 52-53 0.3335968727620937 1106 54-55 0.24735747621553372 1106 56-57 1.5429238133307024 1106 58-59 -8.586948835611263 1106 60-61 -10.106984705008863 1106 62-63 -3.934674233394656 1106 64-65 -5.382823337730169 1106 66-67 -7.134588342972986 1106 68-69 -4.37197755506196 1106 70-71 -4.370897378552854 1106 72-73 -4.821887693743523 1106 74-75 -5.283822164624729 1106 76-77 -6.779398468816819 1106 78-79 -9.553944647332541 1106 80-81 -5.221834344394697 1106 82-83 -2.7411542323445595 1106 84-85 -2.1711981737841874 1106 86-87 -2.045556268760677 1106 88-89 -1.916282495226831 1106 90-91 -4.551701152852068 1106 92-93 -6.969263426075662 1106 94-95 -7.600563345348638 1106 96-97 -7.515012179906206 1106 98-99 -7.14677888547458 1106 100-101 -7.53691390916852 1107 1 0.07485549020062265 1107 2 -0.05151762568059226 1107 3 0.011505501440673527 1107 4 0.04416903884798273 1107 5 -0.06038097547141774 1107 6 0.021893419481841647 1107 7 0.07344244122286625 1107 8 0.021994730823976738 1107 9 0.09661139817731623 1107 10-11 0.0722198386740942 1107 12-13 -0.008173426721263866 1107 14-15 0.08485961181605006 1107 16-17 0.039883532856144654 1107 18-19 0.04536421150189085 1107 20-21 0.13627170386745036 1107 22-23 0.19088260491891162 1107 24-25 0.19721014549075022 1107 26-27 0.149016211849478 1107 28-29 0.12176908438136991 1107 30-31 0.049987645144639714 1107 32-33 0.05706468459081293 1107 34-35 0.07101968231205547 1107 36-37 0.074205901411176 1107 38-39 0.06635983547313629 1107 40-41 -0.06508820882296362 1107 42-43 -0.11124361648224834 1107 44-45 -0.12879895999708424 1107 46-47 0.008710622292923631 1107 48-49 -0.2647997819879322 1107 50-51 -0.2184028685866295 1107 52-53 -0.1986842630064274 1107 54-55 -0.33466016403676946 1107 56-57 1.0010219213849965 1107 58-59 -9.255350177179594 1107 60-61 -10.751701927500324 1107 62-63 -4.4223725476995845 1107 64-65 -5.945176914807423 1107 66-67 -7.431785597848428 1107 68-69 -4.2391800413400915 1107 70-71 -4.160710372342795 1107 72-73 -2.413718722254197 1107 74-75 -4.972471335677508 1107 76-77 -6.9910009592274776 1107 78-79 -7.906238716030156 1107 80-81 -2.988892333131684 1107 82-83 -1.8165071876644383 1107 84-85 -1.5824180767825204 1107 86-87 -1.5052761586100658 1107 88-89 -1.38159853018049 1107 90-91 -0.8383799071583482 1107 92-93 -3.4097296877547443 1107 94-95 -6.071164458876652 1107 96-97 -6.608352519750147 1107 98-99 -6.1677204158617975 1107 100-101 -6.897544645396875 1108 1 -0.0363175695674407 1108 2 -0.07611521317598147 1108 3 -0.06504790218274081 1108 4 -0.09963122912847666 1108 5 -0.05644408607619056 1108 6 -0.09509612657033983 1108 7 -0.11492046625001962 1108 8 -0.07753201129167309 1108 9 -0.061180160564340724 1108 10-11 -0.09549401406066238 1108 12-13 -0.0792287004477501 1108 14-15 -0.17552680450042146 1108 16-17 -0.10581388661656632 1108 18-19 -0.10170291516713803 1108 20-21 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2.9532511670769424 2316 92-93 5.087349256877051 2316 94-95 11.960500672672977 2316 96-97 13.765444517969167 2316 98-99 13.359008633504594 2316 100-101 12.12961986962994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 40.0 5 92.0 6 243.0 7 672.0 8 1363.0 9 2569.0 10 4323.0 11 6767.0 12 10317.0 13 14459.0 14 18131.0 15 24769.0 16 33737.0 17 46034.0 18 50586.0 19 52031.0 20 54409.0 21 54401.0 22 54855.0 23 46650.0 24 47110.0 25 55514.0 26 78260.0 27 117545.0 28 167540.0 29 226910.0 30 282665.0 31 308156.0 32 307576.0 33 282800.0 34 251559.0 35 224953.0 36 184415.0 37 119540.0 38 47477.0 39 3497.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76540390506263 20.147520396824522 14.505501759525721 23.58157393858713 2 15.148609357610685 21.409446618837354 40.17005208799864 23.27189193555332 3 18.64698624566229 25.783333511419627 30.742952968248048 24.82672727467003 4 12.527848200320365 17.677718579891845 38.452908139358385 31.341525080429406 5 12.749546650754596 38.43023142369602 35.38751997663544 13.432701948913945 6 30.590641119075745 38.08027887012596 17.919234640788464 13.409845370009835 7 26.82545030110164 33.42834748908071 22.14094862887345 17.605253580944204 8 25.0292196231415 36.23946671477153 20.43918531385128 18.292128348235682 9 25.819213993286578 16.54647432120159 20.79998541789821 36.83432626761362 10-11 24.09390622137202 27.02812994286896 28.68959387903707 20.188369956721957 12-13 25.12467854165154 24.46969389842749 28.75383055157743 21.65179700834354 14-15 22.130484738051884 25.881389053182847 27.018374803501988 24.96975140526329 16-17 21.444897725726378 28.858592267777674 27.60552146255967 22.090988543936284 18-19 21.314115532763324 27.88991007330183 29.59798150868618 21.197992885248667 20-21 22.378356515418215 27.094959338249136 29.308516104580068 21.21816804175258 22-23 21.78194158152819 27.103466937023036 29.226992183780315 21.88759929766846 24-25 21.73414584591394 27.111424123616057 29.488056420189746 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21.80645035440799 27.69240111137714 28.595701798295302 21.90544673591956 52-53 22.035625962934 27.74059902751923 28.282452881111116 21.941322128435658 54-55 22.032081827641676 27.57630336061414 28.301535777970088 22.090079033774092 56-57 21.106840488369055 27.671660097970257 29.218755209776106 22.002744203884582 58-59 20.849027351420293 27.221993542393886 30.220252204933036 21.70872690125279 60-61 21.86922985911319 27.554650525239303 28.788762670121663 21.787356945525843 62-63 21.912090592148335 27.564089871806114 28.495019047268844 22.02880048877671 64-65 21.49119515586079 27.664741464931108 28.743008616603237 22.101054762604864 66-67 21.57991084672544 27.315476621453556 29.185395112268154 21.91921741955285 68-69 21.896489938027276 26.79074154518443 29.67962018752796 21.633148329260333 70-71 21.787840153921447 26.718912334986033 29.57712332141382 21.916124189678705 72-73 21.003187918027916 26.942886188276148 30.077820189028547 21.976105704667386 74-75 21.454339730474352 27.272381775243915 29.236392104190028 22.036886390091702 76-77 19.642463183721773 27.727618202314826 29.90216332297836 22.727755290985048 78-79 20.517540369785905 27.701455526769468 29.234631752806006 22.546372350638613 80-81 21.49435969208357 27.631998266818457 28.192310830639517 22.681331210458456 82-83 21.869183050893266 27.60804381432526 27.991563724770764 22.531209410010707 84-85 21.929078032015326 27.581320537929887 27.800429670116934 22.68917175993785 86-87 21.61985236185519 27.611849769749487 27.974298303974678 22.79399956442065 88-89 21.780695391794282 27.45542392525902 27.89042092083661 22.873459762110084 90-91 21.176764155351343 27.558546299379856 28.39761356622571 22.86707597904309 92-93 20.216715110959097 27.726900402489402 29.054332913889482 23.002051572662022 94-95 19.56745122677517 27.634264074407973 29.51304868775483 23.285236011062032 96-97 19.627614310772763 27.654748967545732 29.211257117347746 23.506379604333763 98-99 19.818396805069344 27.664364569760934 28.812931364182525 23.704307260987196 100-101 20.234261051601585 27.568839606026174 28.561928279960895 23.63497106241134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 570.0 1 438.0 2 313.0 3 350.5 4 401.0 5 474.0 6 623.0 7 875.0 8 1203.0 9 1451.5 10 1625.5 11 1864.0 12 2140.0 13 2425.5 14 2803.5 15 3146.5 16 3451.5 17 3869.0 18 4369.0 19 4911.0 20 5655.0 21 6841.5 22 8418.0 23 10332.5 24 12944.0 25 16678.0 26 21436.0 27 26848.5 28 32413.0 29 38361.0 30 45111.0 31 52265.5 32 59743.5 33 67964.5 34 76217.0 35 83655.5 36 90656.5 37 96643.5 38 101342.0 39 104925.0 40 108279.0 41 111988.5 42 114927.0 43 117404.5 44 119457.0 45 121062.5 46 122634.5 47 123678.5 48 123390.5 49 120457.0 50 115852.0 51 111002.5 52 105951.0 53 100432.5 54 93148.5 55 84788.5 56 76167.0 57 66980.0 58 57795.0 59 48568.5 60 39111.5 61 30159.5 62 22602.0 63 16298.5 64 11432.5 65 7974.5 66 5289.5 67 3495.0 68 2339.5 69 1522.5 70 954.5 71 546.0 72 330.5 73 210.0 74 123.0 75 72.5 76 41.0 77 18.5 78 6.5 79 3.5 80 3.5 81 4.0 82 4.0 83 2.5 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022878814877389353 2 0.00791958976525016 3 1.571347175644873E-4 4 0.0 5 0.14208121162180942 6 0.0 7 3.1426943512897464E-5 8 0.0014456394015932832 9 0.0 10-11 0.0 12-13 0.0 14-15 5.342580397192568E-4 16-17 0.0 18-19 7.856735878224366E-5 20-21 0.0 22-23 3.142694351289746E-4 24-25 4.714041526934619E-5 26-27 9.428083053869238E-5 28-29 0.0034255368429058236 30-31 0.013042181557852445 32-33 0.0027027171421091815 34-35 0.004588333752883029 36-37 0.11076426241120711 38-39 0.10988430799284596 40-41 0.12232937762395336 42-43 0.12503209476606253 44-45 0.1206951765612827 46-47 0.11398552412127907 48-49 0.11354554691209852 50-51 0.1126341655502245 52-53 0.09104385535686395 54-55 0.07918018418074516 56-57 0.5612694976685922 58-59 1.5864949624180897 60-61 0.5251913665157859 62-63 0.005091164849089389 64-65 0.1217636926407212 66-67 1.9050070349213053 68-69 2.875895314337003 70-71 2.779933142320371 72-73 2.6360763083900824 74-75 2.0097373241780363 76-77 1.4244890686091032 78-79 0.9427140245563851 80-81 0.05356722521773372 82-83 3.142694351289746E-4 84-85 2.1998860459028223E-4 86-87 4.3997720918056445E-4 88-89 2.1998860459028223E-4 90-91 0.25273547973072136 92-93 0.399342171218388 94-95 2.0091244987795345 96-97 2.4275742516537644 98-99 1.4343099884568837 100-101 0.9490936940895034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3181983.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.49386432957015 #Duplication Level Percentage of deduplicated Percentage of total 1 71.12097287976431 36.62293728457621 2 14.934888625865678 15.381102573551352 3 5.699918464490068 8.805324843001902 4 2.7999145787870208 5.767136858177776 5 1.5551945740680608 4.004148920157218 6 0.9648189067979719 2.9809352333555377 7 0.6503083032804006 2.3440821279059736 8 0.4267473194789467 1.7579894857805287 9 0.3062009314892734 1.4190722301326986 >10 1.424110880520718 12.540130551857784 >50 0.07527726474223179 2.633576010469786 >100 0.03859117848472371 3.669930883551267 >500 0.002322630096055117 0.8368307050700953 >1k 5.500966008941047E-4 0.4759812975403739 >5k 1.2224368908757882E-4 0.4313196919588232 >10k+ 6.112184454378941E-5 0.3295013029127934 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10469 0.32900867163652353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7993 0.2511955594985894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5711 0.17947927440215738 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3483 0.10946004425542186 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.1426943512897464E-5 7 0.0 0.0 0.0 0.0 3.1426943512897464E-5 8 0.0 0.0 0.0 0.0 3.1426943512897464E-5 9 0.0 0.0 0.0 0.0 3.1426943512897464E-5 10-11 0.0 0.0 0.0 1.5713471756448732E-5 4.71404152693462E-5 12-13 0.0 0.0 0.0 3.1426943512897464E-5 6.285388702579493E-5 14-15 0.0 0.0 0.0 3.1426943512897464E-5 6.285388702579493E-5 16-17 0.0 0.0 0.0 4.71404152693462E-5 9.428083053869238E-5 18-19 0.0 0.0 0.0 1.4142124580803858E-4 9.428083053869238E-5 20-21 0.0 0.0 0.0 3.456963786418721E-4 9.428083053869238E-5 22-23 0.0 0.0 0.0 5.656849832321543E-4 9.428083053869238E-5 24-25 0.0 0.0 0.0 9.428083053869238E-4 9.428083053869238E-5 26-27 0.0 0.0 0.0 0.001335645099298142 9.428083053869238E-5 28-29 0.0 0.0 0.0 0.0033941098993929258 9.428083053869238E-5 30-31 0.0 0.0 0.0 0.009695212073728867 9.428083053869238E-5 32-33 0.0 0.0 0.0 0.022250276007131402 1.2570777405158985E-4 34-35 0.0 0.0 0.0 0.04052504365988128 1.2570777405158985E-4 36-37 0.0 0.0 0.0 0.06882500629324544 1.2570777405158985E-4 38-39 0.0 0.0 0.0 0.11030857173027009 1.2570777405158985E-4 40-41 0.0 0.0 0.0 0.1566161729965245 1.2570777405158985E-4 42-43 0.0 0.0 0.0 0.20622360333163314 1.2570777405158985E-4 44-45 0.0 0.0 0.0 0.26705045250084614 1.2570777405158985E-4 46-47 0.0 0.0 0.0 0.33457124063830634 1.4142124580803858E-4 48-49 0.0 0.0 0.0 0.39797509917557694 2.1998860459028223E-4 50-51 0.0 0.0 0.0 0.4590533638928932 2.1998860459028223E-4 52-53 3.1426943512897464E-5 0.0 0.0 0.5206658866499287 2.1998860459028223E-4 54-55 6.285388702579493E-5 0.0 0.0 0.58906662920575 2.1998860459028223E-4 56-57 6.285388702579493E-5 0.0 0.0 0.6457765487747734 2.1998860459028223E-4 58-59 6.285388702579493E-5 3.1426943512897464E-5 0.0 0.6856259131491275 2.1998860459028223E-4 60-61 6.285388702579493E-5 3.1426943512897464E-5 0.0 0.7177128224757958 2.1998860459028223E-4 62-63 2.042751328338335E-4 3.1426943512897464E-5 0.0 0.7477726939458822 2.1998860459028223E-4 64-65 2.3570207634673098E-4 3.1426943512897464E-5 0.0 0.7779582731900201 2.1998860459028223E-4 66-67 2.514155481031797E-4 3.1426943512897464E-5 0.0 0.8044040461561235 2.1998860459028223E-4 68-69 2.8284249161607717E-4 3.1426943512897464E-5 0.0 0.8371823482400754 2.3570207634673098E-4 70-71 2.8284249161607717E-4 3.1426943512897464E-5 0.0 0.8922894936899411 2.514155481031797E-4 72-73 2.8284249161607717E-4 3.1426943512897464E-5 0.0 0.9709511333027235 2.6712901985962844E-4 74-75 2.985559633725259E-4 3.1426943512897464E-5 0.0 1.0727430033409984 2.8284249161607717E-4 76-77 3.142694351289746E-4 3.1426943512897464E-5 0.0 1.240437173925819 2.8284249161607717E-4 78-79 3.142694351289746E-4 3.1426943512897464E-5 0.0 1.5009193952324698 2.8284249161607717E-4 80-81 3.142694351289746E-4 3.1426943512897464E-5 0.0 1.8092019976222375 2.985559633725259E-4 82-83 3.142694351289746E-4 6.285388702579493E-5 0.0 2.1161646683844637 3.456963786418721E-4 84-85 3.142694351289746E-4 1.0999430229514113E-4 0.0 2.3516938965418737 3.456963786418721E-4 86-87 3.142694351289746E-4 1.2570777405158985E-4 0.0 2.533860174614383 3.456963786418721E-4 88-89 3.142694351289746E-4 1.2570777405158985E-4 0.0 2.7144708189830054 3.7712332215476954E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14055 0.0 28.4732 1 GGTATCA 9820 0.0 28.430952 1 TACATGG 10365 0.0 24.341537 2 ATCAACG 16410 0.0 24.121202 3 TCAACGC 16560 0.0 23.873915 4 GTACATG 11000 0.0 23.62624 1 CAACGCA 16880 0.0 23.421328 5 ACATGGG 10310 0.0 23.236494 3 AACGCAG 17120 0.0 23.114511 6 TATCAAC 17255 0.0 23.023958 2 GAGTACT 9210 0.0 20.615377 12-13 CGCAGAG 18815 0.0 20.357748 8 ACGCAGA 19110 0.0 20.338606 7 CATGGGG 4920 0.0 19.897703 4 GCAGAGT 19775 0.0 18.775007 9 AGTACTT 9765 0.0 18.625513 12-13 AGAGTAC 14800 0.0 18.576492 10-11 CATGGGA 6025 0.0 18.513817 4 GTACTTT 10255 0.0 18.377153 14-15 AGTACAT 7845 0.0 17.400333 2 >>END_MODULE