FastQCFastQC Report
Fri 27 May 2016
SRR939692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996081
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39610.13220603848827853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115400.035.7380141
GGTATCA82650.028.04461
GTACATG94900.027.61511
TATCAAC153200.025.9876582
TACATGG101450.025.4644092
ATCAACG160300.024.8370293
TCAACGC161450.024.5694794
CAACGCA161650.024.3150675
ACATGGG100550.024.2836553
AACGCAG166150.023.7133166
ACGCAGA174100.022.2510497
CATGGGG50000.021.1559584
GAGTACT78550.021.05517612-13
CGCAGAG184800.020.7322148
AGAGTAC135150.019.84800710-11
GTATAGG7903.6379788E-1219.8245491
AGTACTT87900.019.40601712-13
GCAGAGT184450.019.1347059
CATGGGA59450.018.5874024
GTACTTT88400.018.55190514-15
Produced by FastQC (version 0.11.5)