##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939692_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2996081 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.78848469050069 2.0 2.0 31.0 2.0 33.0 2 22.338234847455727 16.0 16.0 31.0 16.0 34.0 3 26.99525713757405 28.0 25.0 31.0 16.0 34.0 4 32.29332885192356 35.0 32.0 35.0 25.0 37.0 5 32.9205295183942 35.0 33.0 35.0 28.0 37.0 6 33.32295321788697 35.0 33.0 37.0 28.0 37.0 7 33.49738074504661 35.0 33.0 37.0 28.0 37.0 8 33.42935187666822 35.0 33.0 37.0 27.0 37.0 9 34.80253504494705 37.0 34.0 39.0 27.0 39.0 10-11 34.651127923443994 37.0 34.0 39.0 27.0 39.0 12-13 34.66268235071081 37.0 34.0 39.0 27.0 39.0 14-15 35.36629617156545 38.0 34.0 40.0 27.0 41.0 16-17 34.97564902284017 38.0 33.0 40.0 25.5 41.0 18-19 35.22564259778024 38.0 33.5 40.0 27.0 41.0 20-21 35.225959678660224 38.0 34.0 40.0 26.0 41.0 22-23 35.046406288748535 38.0 34.0 40.0 25.5 41.0 24-25 34.85824698998458 38.0 33.0 40.0 25.0 41.0 26-27 34.95661582580711 38.0 34.0 40.0 25.0 41.0 28-29 34.70284998970322 38.0 33.0 40.0 24.0 41.0 30-31 34.57968159071801 38.0 33.0 40.0 24.0 41.0 32-33 34.28444491320495 38.0 33.0 40.0 24.0 41.0 34-35 34.01654778358796 38.0 33.0 40.0 23.0 41.0 36-37 33.75546038308043 38.0 32.0 40.0 21.0 41.0 38-39 33.55637764800083 38.0 32.0 40.0 19.0 41.0 40-41 33.18799124589756 37.0 31.0 40.0 17.0 41.0 42-43 32.95055958099931 37.0 31.0 40.0 16.0 41.0 44-45 32.61548586303241 37.0 30.5 40.0 15.0 41.0 46-47 32.29928363084977 37.0 30.0 39.5 14.0 41.0 48-49 31.93118160023043 36.5 30.0 39.0 9.0 40.0 50-51 31.285917837334836 35.5 29.5 38.5 9.0 40.0 52-53 31.34270885867238 36.0 30.0 39.0 8.5 40.0 54-55 31.47379209707615 36.0 30.0 39.0 8.0 40.0 56-57 31.196038257977673 36.0 29.0 39.0 7.0 40.0 58-59 30.790149698889984 35.5 28.5 39.0 4.0 40.0 60-61 30.220627212682167 35.0 28.0 38.0 2.0 40.0 62-63 29.8240226148759 34.5 28.0 38.0 2.0 40.0 64-65 29.254830059667945 34.0 26.5 38.0 2.0 40.0 66-67 28.667589594540335 34.0 26.0 37.5 2.0 39.0 68-69 28.32093141006535 34.0 26.0 37.0 2.0 39.0 70-71 27.811216719441163 33.0 25.5 36.0 2.0 39.0 72-73 27.218021141618003 32.5 24.0 36.0 2.0 39.0 74-75 26.650039835371608 32.0 22.5 35.5 2.0 38.0 76-77 26.00862877205256 31.5 21.5 35.0 2.0 37.0 78-79 25.330660119002122 31.0 19.0 34.5 2.0 36.5 80-81 24.595746243175668 31.0 17.5 34.0 2.0 36.0 82-83 23.896871780168826 30.0 10.5 34.0 2.0 35.5 84-85 23.18918530573773 29.5 7.0 34.0 2.0 35.0 86-87 22.498186464251134 29.0 2.0 34.0 2.0 35.0 88-89 21.69310909818526 29.0 2.0 33.5 2.0 35.0 90-91 20.782566626202694 27.5 2.0 33.0 2.0 34.0 92-93 19.759218959701023 26.0 2.0 32.0 2.0 34.0 94-95 18.76946684685761 25.0 2.0 32.0 2.0 34.0 96-97 17.766679705922503 23.5 2.0 32.0 2.0 34.0 98-99 16.645103887378212 19.0 2.0 31.0 2.0 34.0 100-101 15.05170153944436 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.864657925374168 1101 2 8.058238304909992 1101 3 3.9160882301956725 1101 4 2.507142379132695 1101 5 1.7685118801873543 1101 6 1.530798118544979 1101 7 1.2218476996818879 1101 8 1.199089733940987 1101 9 1.1577912907965668 1101 10-11 1.1494541418375448 1101 12-13 0.922409386263304 1101 14-15 1.1145978925796172 1101 16-17 1.1479810720454608 1101 18-19 1.1526878125085958 1101 20-21 1.0591732925684099 1101 22-23 0.9942881362058458 1101 24-25 1.14831853765007 1101 26-27 1.3272373015280152 1101 28-29 1.3288018670701334 1101 30-31 1.2764621465530013 1101 32-33 1.2535878297928562 1101 34-35 1.2516387867971233 1101 36-37 1.337008416105057 1101 38-39 1.3015512619988314 1101 40-41 1.3176669385727493 1101 42-43 1.2372921561848145 1101 44-45 0.8938726526873495 1101 46-47 0.6935293130747624 1101 48-49 0.6727785972922149 1101 50-51 0.5896807052272379 1101 52-53 0.825259710452265 1101 54-55 0.989583827015867 1101 56-57 0.5639472164341868 1101 58-59 0.5744045468823522 1101 60-61 0.5584988728062754 1101 62-63 0.3834210057232674 1101 64-65 0.5058919664184636 1101 66-67 0.4925038067646845 1101 68-69 0.36253308481692414 1101 70-71 -0.036153771211346 1101 72-73 0.053639703721124476 1101 74-75 -0.12429862644161815 1101 76-77 -0.14101712831531543 1101 78-79 -0.16400964534418705 1101 80-81 -0.14707278568543103 1101 82-83 -0.04764415384534715 1101 84-85 -0.30489936512871907 1101 86-87 -0.45416873710633254 1101 88-89 -0.6578392577835963 1101 90-91 -0.2709934633467732 1101 92-93 -0.49225761803545254 1101 94-95 -0.5008191537773818 1101 96-97 -1.2597117812388525 1101 98-99 -1.516081048281217 1101 100-101 -1.4115041878175916 1102 1 14.816127322244295 1102 2 7.963587513609145 1102 3 3.714209400736035 1102 4 2.21584210878909 1102 5 1.712207194366023 1102 6 1.3454822750735218 1102 7 1.0493831142327465 1102 8 1.0286364518844167 1102 9 1.1179798880533411 1102 10-11 1.0234982470739666 1102 12-13 0.7871858367415072 1102 14-15 0.8164055309816547 1102 16-17 0.9915448915882692 1102 18-19 0.9567430433279611 1102 20-21 0.9638259522144779 1102 22-23 0.9441985785274554 1102 24-25 0.8664957988710427 1102 26-27 0.985165085856849 1102 28-29 1.1218318448900106 1102 30-31 0.9633796688084857 1102 32-33 0.8927792126697867 1102 34-35 0.8897359011926582 1102 36-37 0.8620718447931282 1102 38-39 0.7423303830777783 1102 40-41 0.8867512593159645 1102 42-43 0.8640889983165536 1102 44-45 0.7045802389946232 1102 46-47 0.44623308511590665 1102 48-49 0.26442683493190344 1102 50-51 0.21701188833140606 1102 52-53 0.40398141860752546 1102 54-55 0.4425982090496454 1102 56-57 0.4147391125395963 1102 58-59 0.30183050098174036 1102 60-61 0.4953985372914147 1102 62-63 -0.03232454562771281 1102 64-65 0.07912202615366581 1102 66-67 0.15868096890383043 1102 68-69 -0.10207353442488198 1102 70-71 -0.0010700691534601958 1102 72-73 0.13759729094049078 1102 74-75 0.016984563808726705 1102 76-77 -0.15060723103973928 1102 78-79 -0.17720068105070297 1102 80-81 -0.271322543309406 1102 82-83 -0.18474147312518951 1102 84-85 -0.5080764352907465 1102 86-87 -0.4273719045755442 1102 88-89 -0.4194735951652646 1102 90-91 -0.2809272801456366 1102 92-93 -0.12331766680444645 1102 94-95 -0.23307313850841282 1102 96-97 -0.9037533249413272 1102 98-99 -1.0495603352838145 1102 100-101 -1.0441549222808035 1103 1 14.70597856652634 1103 2 7.849811113438253 1103 3 3.7595686123193026 1103 4 2.1438308117190132 1103 5 1.58074776931376 1103 6 1.0016518113881006 1103 7 0.7837102270615901 1103 8 0.8484644039950524 1103 9 0.9097105494337754 1103 10-11 0.7380595283003899 1103 12-13 0.4889308498451541 1103 14-15 0.6546948954991691 1103 16-17 0.8327495631025172 1103 18-19 0.7745078829716832 1103 20-21 0.6778900928700935 1103 22-23 0.7462330507181889 1103 24-25 0.9248943252949147 1103 26-27 1.0223829064442356 1103 28-29 0.9843113737811606 1103 30-31 0.9048925646897032 1103 32-33 0.8490660505631311 1103 34-35 0.8037162297912843 1103 36-37 0.8821770306896966 1103 38-39 0.8658340726383642 1103 40-41 0.6495287659880944 1103 42-43 0.6329698193737201 1103 44-45 0.5718389223724216 1103 46-47 0.5606372860861057 1103 48-49 0.560727724489773 1103 50-51 0.38984884842666645 1103 52-53 0.6178943687036806 1103 54-55 0.5076853036371567 1103 56-57 0.711707795238322 1103 58-59 0.42263487005912026 1103 60-61 0.5887140008760419 1103 62-63 0.46377952215545193 1103 64-65 0.3168151378374624 1103 66-67 0.41513104615268404 1103 68-69 0.393438265824102 1103 70-71 0.2491901133100889 1103 72-73 0.24619586732460874 1103 74-75 0.2653346358646189 1103 76-77 0.24966617537227975 1103 78-79 0.2080951057445013 1103 80-81 0.14802279738772484 1103 82-83 0.0687612939639628 1103 84-85 -0.03011137940040598 1103 86-87 -0.24098757362140333 1103 88-89 -0.2832028266685249 1103 90-91 -0.27908317335856125 1103 92-93 -0.013397252957563666 1103 94-95 0.0495039679801863 1103 96-97 -0.5857730097199294 1103 98-99 -1.0097841196072057 1103 100-101 -0.9677102349405562 1104 1 14.819436092809013 1104 2 8.063602473034816 1104 3 3.7719959200537403 1104 4 2.245983277653906 1104 5 1.606687664020093 1104 6 1.2116908127855126 1104 7 1.0481125308510926 1104 8 1.0319775746266444 1104 9 1.148286412492098 1104 10-11 0.9190318215831041 1104 12-13 0.7998427275202715 1104 14-15 0.752179130996474 1104 16-17 1.0077448988329536 1104 18-19 0.8297228641411394 1104 20-21 1.046395069047037 1104 22-23 1.0482115205045162 1104 24-25 1.0151736870425623 1104 26-27 0.833808741553355 1104 28-29 0.99404378538744 1104 30-31 0.8839210617605247 1104 32-33 0.9528512612456055 1104 34-35 1.004428103405374 1104 36-37 1.0250507779952827 1104 38-39 0.7904913000435627 1104 40-41 0.986790966774862 1104 42-43 0.748338990505026 1104 44-45 0.5805359071099474 1104 46-47 0.6166477653726972 1104 48-49 0.5824302133840789 1104 50-51 0.6889933817798095 1104 52-53 0.6347475651783725 1104 54-55 0.5895494034395021 1104 56-57 0.4905159311813101 1104 58-59 0.46822037679638484 1104 60-61 0.43911860634257494 1104 62-63 0.336770801769692 1104 64-65 0.22199283671950099 1104 66-67 0.4343553942862961 1104 68-69 0.4434116915523134 1104 70-71 0.3873969049418484 1104 72-73 0.2847564241122882 1104 74-75 0.18425264915985906 1104 76-77 0.17980279271560562 1104 78-79 0.11046727721443261 1104 80-81 0.10679657298395284 1104 82-83 -0.06681515921892611 1104 84-85 -0.030706921715417224 1104 86-87 0.023062485623242424 1104 88-89 -0.14656431315962593 1104 90-91 -0.29087792510150123 1104 92-93 0.10720473237773476 1104 94-95 0.20728562225387392 1104 96-97 -0.3160158786231335 1104 98-99 -0.4675428645549289 1104 100-101 -0.5223357289228989 1105 1 14.776659494399372 1105 2 8.04734941358577 1105 3 3.796886906349549 1105 4 2.1896759013637634 1105 5 1.4861946657663836 1105 6 1.2778738629344488 1105 7 1.0240039000712002 1105 8 0.9882574514741265 1105 9 1.002007154200676 1105 10-11 1.0137225970619497 1105 12-13 0.8830774728121256 1105 14-15 0.7820078181756216 1105 16-17 1.078782979212228 1105 18-19 0.9567951856800079 1105 20-21 0.9956354241526313 1105 22-23 0.9013601432014582 1105 24-25 0.9906546506622078 1105 26-27 0.8835195620579981 1105 28-29 1.0850777789432087 1105 30-31 1.0690790175796607 1105 32-33 0.6439956516404735 1105 34-35 0.9351689158978118 1105 36-37 1.0291053585843386 1105 38-39 0.988772303715173 1105 40-41 1.3057706703359528 1105 42-43 1.201366505003449 1105 44-45 0.8885158789538536 1105 46-47 0.7322345800792363 1105 48-49 0.5823336660334277 1105 50-51 0.5900983750509745 1105 52-53 0.9622019403197442 1105 54-55 0.8920941539754743 1105 56-57 0.7172290185760275 1105 58-59 0.6961959715852011 1105 60-61 0.9086892758288414 1105 62-63 0.9028926765423826 1105 64-65 0.8153857781785199 1105 66-67 0.760477969242686 1105 68-69 0.6513986743029427 1105 70-71 0.6014553510528273 1105 72-73 0.6663514153681511 1105 74-75 0.5699876579275731 1105 76-77 0.4678412698051737 1105 78-79 0.44763828837583475 1105 80-81 0.4204790966346543 1105 82-83 0.5069684666043592 1105 84-85 0.4213850947574045 1105 86-87 0.36009790421609367 1105 88-89 0.37326916102672314 1105 90-91 0.711978815318453 1105 92-93 0.9474872982208531 1105 94-95 0.8050017899126765 1105 96-97 0.3337324180349057 1105 98-99 0.09130771134616111 1105 100-101 0.10738949069624759 1106 1 14.726600953773495 1106 2 7.866179792716284 1106 3 3.4936382397343024 1106 4 2.1400086672630323 1106 5 1.5436059674883893 1106 6 1.1856521812996803 1106 7 0.8620388212085857 1106 8 1.0197655771615644 1106 9 1.1093534453056861 1106 10-11 0.9567276410962648 1106 12-13 0.6474059443918705 1106 14-15 0.9201148047790824 1106 16-17 1.0618846966110524 1106 18-19 0.8205196269304835 1106 20-21 0.7513422670272618 1106 22-23 0.7306851586362271 1106 24-25 0.9443214724380766 1106 26-27 0.7700954088135177 1106 28-29 0.8424961537911102 1106 30-31 0.6959076582199017 1106 32-33 0.7323977539123874 1106 34-35 0.8044997689460374 1106 36-37 0.9970472836516038 1106 38-39 1.0761592205158408 1106 40-41 1.0271969780509167 1106 42-43 0.7454230322558217 1106 44-45 0.7054634723913153 1106 46-47 0.8482059724834698 1106 48-49 0.7351465403653705 1106 50-51 0.722296011713091 1106 52-53 0.6431712473398363 1106 54-55 0.5641529672913741 1106 56-57 0.5817934620635796 1106 58-59 0.4808415860461075 1106 60-61 0.786959774094921 1106 62-63 0.7418329395164527 1106 64-65 0.6660967644062126 1106 66-67 0.5802868888382413 1106 68-69 0.5455344498081374 1106 70-71 0.5135382239628292 1106 72-73 0.622620306736998 1106 74-75 0.3766336181857426 1106 76-77 0.5605973653958252 1106 78-79 0.3928047480880785 1106 80-81 0.472288281609508 1106 82-83 0.5631637207013789 1106 84-85 0.3435609710482481 1106 86-87 0.3154350437562563 1106 88-89 0.2968197276626974 1106 90-91 0.6210910667401137 1106 92-93 0.8966076718573568 1106 94-95 0.8492691401808159 1106 96-97 0.4198935091166618 1106 98-99 0.06476320201091568 1106 100-101 0.08805737654805945 1107 1 14.683804442092121 1107 2 7.869121616746359 1107 3 3.611631358377924 1107 4 2.1905703718169534 1107 5 1.3922246021841502 1107 6 1.0769762662814202 1107 7 0.6134747512222276 1107 8 0.809439539498932 1107 9 0.6604723374076684 1107 10-11 0.6342912312521847 1107 12-13 0.45127565699309 1107 14-15 0.5495026792600584 1107 16-17 0.8232360855858474 1107 18-19 0.6039169797306343 1107 20-21 0.697171601022248 1107 22-23 0.5354053936864247 1107 24-25 0.6849745611594642 1107 26-27 0.7044036280150721 1107 28-29 0.6448104295672081 1107 30-31 0.6734387547848328 1107 32-33 0.716483287430286 1107 34-35 0.5088547486249198 1107 36-37 0.6681226592257659 1107 38-39 0.5541731678758666 1107 40-41 0.7206824728722339 1107 42-43 0.5353483601083937 1107 44-45 0.5789062999900949 1107 46-47 0.6370006513964839 1107 48-49 0.4450664983004131 1107 50-51 0.6269583822053022 1107 52-53 0.5159076729258167 1107 54-55 0.5131813061385806 1107 56-57 0.4112691431712605 1107 58-59 0.5987341314357089 1107 60-61 0.5756855709227047 1107 62-63 0.3249519780379444 1107 64-65 0.2828824179628633 1107 66-67 0.5197712270896737 1107 68-69 0.561896965403534 1107 70-71 0.5385300239292512 1107 72-73 0.4892154977215952 1107 74-75 0.4094358261631186 1107 76-77 0.2807164250147096 1107 78-79 0.436690480282099 1107 80-81 0.49486068849201814 1107 82-83 0.4563692603357161 1107 84-85 0.37186357401569836 1107 86-87 0.6001359719874344 1107 88-89 0.5874728254000594 1107 90-91 0.8624082725293114 1107 92-93 1.164037454054725 1107 94-95 1.109887034123684 1107 96-97 0.6481445316640055 1107 98-99 0.558094667834311 1107 100-101 0.46408192994601016 1108 1 14.390006803846639 1108 2 7.479399984558583 1108 3 3.3816162676797497 1108 4 0.14499887355298569 1108 5 0.47405037581118137 1108 6 0.6101536192542341 1108 7 0.5530639325172544 1108 8 0.6291660858714749 1108 9 0.7827266315169368 1108 10-11 0.6231964660417617 1108 12-13 0.36709634147073444 1108 14-15 0.49357416837408863 1108 16-17 0.6678449660220807 1108 18-19 0.5869955803529194 1108 20-21 0.468093597600614 1108 22-23 -0.23458576435170642 1108 24-25 0.3685365908025773 1108 26-27 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6 -0.685259918135948 2312 7 -0.2186714322769845 2312 8 0.09767184467431633 2312 9 0.10047094277252455 2312 10-11 -0.012180150556481806 2312 12-13 -0.6943268166931702 2312 14-15 0.06948300134078522 2312 16-17 -0.339908750577564 2312 18-19 0.06860419193498046 2312 20-21 0.07576004766997357 2312 22-23 0.20907937737649007 2312 24-25 0.20315311723562957 2312 26-27 0.19278068310211438 2312 28-29 -0.47908942589155856 2312 30-31 0.0021811677609875346 2312 32-33 0.2133002761730367 2312 34-35 -0.7518529017661351 2312 36-37 -0.1756226890921866 2312 38-39 0.11326909303716803 2312 40-41 0.2080666329471299 2312 42-43 0.07701847568574038 2312 44-45 0.23409841185026892 2312 46-47 0.40026103186407624 2312 48-49 0.36680377373285467 2312 50-51 0.16995561832364103 2312 52-53 0.11865463463838921 2312 54-55 -0.06500286151812773 2312 56-57 0.1993868952419966 2312 58-59 0.0998909656348026 2312 60-61 0.2850422163417612 2312 62-63 0.12171855471992288 2312 64-65 0.24521335757829021 2312 66-67 -0.054674770620483315 2312 68-69 -0.001784648784045828 2312 70-71 0.14563513351070867 2312 72-73 -0.05074327707230353 2312 74-75 0.05553220414610749 2312 76-77 0.20803624058899217 2312 78-79 0.5355140307658068 2312 80-81 0.3733818379078713 2312 82-83 0.2575954089446988 2312 84-85 0.40491503570461873 2312 86-87 0.40732444666697987 2312 88-89 0.7868440049344692 2312 90-91 0.993015810027952 2312 92-93 1.5562172896477655 2312 94-95 1.7352299109116665 2312 96-97 1.3391492915085301 2312 98-99 1.0625279381945951 2312 100-101 1.0874277135570658 2313 1 -14.198898743379747 2313 2 -7.433751777786902 2313 3 -3.6554987625913746 2313 4 -1.074071626428971 2313 5 -0.5491506778017694 2313 6 -0.9834426536603971 2313 7 -0.370633026988898 2313 8 -0.1970935089347634 2313 9 -2.6821307812329422 2313 10-11 -0.6380645505433122 2313 12-13 -0.8565183485418615 2313 14-15 -0.981318978123717 2313 16-17 -0.8768974895264208 2313 18-19 -0.09594668310138132 2313 20-21 -1.229302126146436 2313 22-23 -2.174380473573727 2313 24-25 -1.1953093781683037 2313 26-27 -0.32600179806087226 2313 28-29 0.12237510755958425 2313 30-31 -1.2818828088114103 2313 32-33 -0.4344234185438225 2313 34-35 -1.2361660777996306 2313 36-37 -1.1936698852166714 2313 38-39 -0.6384724392231078 2313 40-41 -0.21883711648350612 2313 42-43 -0.00864690453936845 2313 44-45 -0.2509832335144324 2313 46-47 -0.8329507171933841 2313 48-49 -0.14369163774551552 2313 50-51 -0.790786505937561 2313 52-53 0.009041342262644747 2313 54-55 -0.7423429801103829 2313 56-57 -0.27877912637460867 2313 58-59 -0.16416768430126538 2313 60-61 -1.5019225132997214 2313 62-63 -0.3292620711409846 2313 64-65 -0.06297559978666456 2313 66-67 -1.4933592012020291 2313 68-69 -0.27897278846059237 2313 70-71 0.11857750156927338 2313 72-73 -0.6904989686501111 2313 74-75 -0.2773928566317423 2313 76-77 0.23086197486221138 2313 78-79 0.4682771659148841 2313 80-81 0.3014538116375718 2313 82-83 0.27744167391964325 2313 84-85 0.3276222154478958 2313 86-87 0.49001232978692855 2313 88-89 0.8140137946403776 2313 90-91 1.1771443030391886 2313 92-93 1.7678145739893019 2313 94-95 1.8551654047625412 2313 96-97 1.4362876907599897 2313 98-99 1.354955128255014 2313 100-101 1.4523392598387783 2314 1 -14.198898743379747 2314 2 -7.31382766197318 2314 3 -3.6573145627630197 2314 4 -1.486061779552415 2314 5 -1.4333753491961403 2314 6 -6.369067568968223 2314 7 -2.062254148087412 2314 8 -4.598493752848221 2314 9 -1.6585005359644072 2314 10-11 -0.6257491794786745 2314 12-13 -3.209738909542807 2314 14-15 -3.023143360435469 2314 16-17 -2.6189567514623704 2314 18-19 -1.299447292885013 2314 20-21 -2.0509302484645175 2314 22-23 -1.184030864286946 2314 24-25 -2.9118729085897357 2314 26-27 -4.879454980228083 2314 28-29 -2.176971068871147 2314 30-31 -2.1708129772923783 2314 32-33 -4.328406886631804 2314 34-35 -3.0372919190752086 2314 36-37 -1.3138808251868639 2314 38-39 -1.876030594514674 2314 40-41 -3.0089699138094943 2314 42-43 -1.876402900745159 2314 44-45 -2.9864353767910004 2314 46-47 -2.6530927741968604 2314 48-49 -1.5686972838903301 2314 50-51 -2.585298453179984 2314 52-53 -1.40039675340563 2314 54-55 -0.7915626828973217 2314 56-57 -1.5827280400563488 2314 58-59 -1.9205049172386133 2314 60-61 -1.1278552564227162 2314 62-63 -3.3161144583310076 2314 64-65 -1.6781829262241743 2314 66-67 -2.8059568795072813 2314 68-69 -2.6422107395875614 2314 70-71 -3.2856266178579787 2314 72-73 -3.3221986840844124 2314 74-75 -2.6822304735069373 2314 76-77 -1.3466686272426713 2314 78-79 -0.8098078876105674 2314 80-81 -0.6298630951784538 2314 82-83 -0.7493368616962357 2314 84-85 -0.523477653561546 2314 86-87 -0.20776534761494148 2314 88-89 0.014733113489466376 2314 90-91 0.5005475732862621 2314 92-93 1.0673911131283802 2314 94-95 0.8763215093743106 2314 96-97 0.7318769224326047 2314 98-99 0.7802351770377065 2314 100-101 0.8103748531356754 2315 1 -14.198898743379747 2315 2 -7.415924414310684 2315 3 -4.541910976331835 2315 4 -2.7226917086368374 2315 5 -3.39033681990394 2315 6 -2.7738595649933373 2315 7 -2.9471802965132667 2315 8 -3.464069433773286 2315 9 -2.3738747624425685 2315 10-11 -4.501511409028339 2315 12-13 -4.1959252774907725 2315 14-15 -3.250902066790772 2315 16-17 -4.3518945535311175 2315 18-19 -1.4308967711812315 2315 20-21 -3.1158552676614093 2315 22-23 -4.023440955076953 2315 24-25 -4.7401009225018385 2315 26-27 -4.533835033527694 2315 28-29 -2.33046106390254 2315 30-31 -3.008001423021632 2315 32-33 -4.217435749724089 2315 34-35 -3.672243588075613 2315 36-37 -4.918385093672338 2315 38-39 -3.3661764908966276 2315 40-41 -4.188355613253911 2315 42-43 -2.846215900428973 2315 44-45 -1.4211182892983416 2315 46-47 -0.8467968710395368 2315 48-49 -0.5501743153732335 2315 50-51 -0.27954164392933833 2315 52-53 -0.08121115333336348 2315 54-55 -1.6912038139330505 2315 56-57 -3.7562600424051453 2315 58-59 -2.9948194752583994 2315 60-61 -4.2341245097765245 2315 62-63 -2.0488745197610605 2315 64-65 -2.643345535194772 2315 66-67 -1.4766122839970954 2315 68-69 -0.9038935165874271 2315 70-71 -1.532127137303302 2315 72-73 -1.7320609181509923 2315 74-75 -2.4408455871073684 2315 76-77 -2.1411932218494734 2315 78-79 -1.3312002269212861 2315 80-81 -1.1285696763248474 2315 82-83 -0.5964103519171147 2315 84-85 -0.21637661015398635 2315 86-87 -0.7580851452571125 2315 88-89 -1.6067436921476457 2315 90-91 -1.394276718687177 2315 92-93 -0.09011261332719656 2315 94-95 0.17807204011192468 2315 96-97 -0.8175232311425376 2315 98-99 -0.33055867092290825 2315 100-101 0.06366045772486295 2316 1 -14.198898743379747 2316 2 -7.375848195333093 2316 3 -5.117713087550754 2316 4 -2.8943480685961553 2316 5 -2.061175579358249 2316 6 -1.1390807987588119 2316 7 -4.4858385151869165 2316 8 -2.901585536785806 2316 9 -1.1787887822922372 2316 10-11 -4.46408929383481 2316 12-13 -3.6155872019030575 2316 14-15 -1.753573238014603 2316 16-17 -3.1406812456041138 2316 18-19 -2.3253414364373057 2316 20-21 -3.1450522796904465 2316 22-23 -2.910364490608792 2316 24-25 -3.4139552413823964 2316 26-27 -4.017917424839663 2316 28-29 -3.5353337010557873 2316 30-31 -2.6108949385635256 2316 32-33 -2.8250246816062976 2316 34-35 -4.001686792078278 2316 36-37 -2.3346244119099495 2316 38-39 -3.2475720463482745 2316 40-41 -2.732760883118189 2316 42-43 -6.358006695701526 2316 44-45 -3.2663593961101185 2316 46-47 -4.410837412054001 2316 48-49 -4.231006879113103 2316 50-51 -3.2610421758255796 2316 52-53 -4.817699235350595 2316 54-55 -4.819856538293902 2316 56-57 -4.8955888010411535 2316 58-59 -3.4996158934340507 2316 60-61 -3.270971008028866 2316 62-63 -2.3062865815311646 2316 64-65 -2.4923136564904915 2316 66-67 -2.895712853301422 2316 68-69 -2.8148243934563695 2316 70-71 -2.9379733199249074 2316 72-73 -3.959723263404257 2316 74-75 -4.51516540287254 2316 76-77 -4.141608685417506 2316 78-79 -4.422932338257105 2316 80-81 -4.2154034324474985 2316 82-83 -3.7069217906087637 2316 84-85 -3.2999979467916987 2316 86-87 -3.5769965699207305 2316 88-89 -3.32101020138861 2316 90-91 -2.475564725888237 2316 92-93 -2.441760322666358 2316 94-95 -1.7188530151234538 2316 96-97 -1.8139783472829585 2316 98-99 -1.9216031748566014 2316 100-101 -1.7542975283172453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19900.0 3 5902.0 4 6743.0 5 7830.0 6 9228.0 7 11367.0 8 14032.0 9 16909.0 10 20169.0 11 24251.0 12 27621.0 13 31100.0 14 33601.0 15 37211.0 16 42051.0 17 44032.0 18 41706.0 19 41978.0 20 45823.0 21 50218.0 22 55799.0 23 61454.0 24 68755.0 25 76925.0 26 86661.0 27 97579.0 28 110084.0 29 125097.0 30 142613.0 31 163324.0 32 186490.0 33 215397.0 34 252001.0 35 294490.0 36 312810.0 37 190023.0 38 24788.0 39 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.592588082031526 19.210094143610522 13.720536297510048 23.476781476847904 2 15.05210424724613 20.672728402477166 41.20942519965444 23.065742150622263 3 18.812826801289777 25.694538895013558 30.25608088080247 25.236553422894193 4 12.205502788046282 16.894048890633808 39.09775919600464 31.802689125315275 5 13.041046010590836 38.082267131578085 35.265429264422714 13.611257593408363 6 30.54853781856422 37.48005265364053 18.440072388727735 13.531337139067517 7 26.970159816458615 33.30775975815755 22.31309477366383 17.40898565172 8 25.147268143348345 35.6633601673017 20.789795826951323 18.39957586239864 9 26.349869846155084 16.631047608097212 20.82658721510881 36.1924953306389 10-11 24.217663316834827 26.801372907469112 28.796249569713154 20.18471420598291 12-13 25.415145585896582 24.4159450933572 28.638026339626222 21.53088298111999 14-15 22.55205078583638 25.671095090070718 27.011906419311398 24.764947704781505 16-17 21.791832907192703 28.630533484391957 27.544438927465958 22.033194680949382 18-19 21.570682570902523 27.694636799649146 29.606357460224924 21.128323169223403 20-21 22.694340763269548 26.939829921634757 29.315547093878802 21.050282221216897 22-23 22.078694522385014 26.956912148807593 29.162823518395275 21.801569810412115 24-25 21.921077337545594 26.98301431121178 29.485080382334804 21.610827968907824 26-27 21.9116698181463 27.130777652268645 29.412436642883115 21.545115886701936 28-29 21.755058761746916 27.445222879869146 29.33304121099205 21.466677147391888 30-31 21.986081571924284 27.167262840172253 29.266903128844568 21.579752459058895 32-33 21.778551608905303 27.21959353795257 29.268495428096426 21.733359425045702 34-35 22.028290464736084 27.24902940287206 29.25285092002708 21.46982921236478 36-37 22.122620214092947 26.97524320383642 29.359660768687984 21.542475813382648 38-39 21.768551997425103 27.409011298608473 29.32055360255483 21.501883101411597 40-41 22.130496269858334 27.23262580638042 29.166367093826885 21.470510829934366 42-43 21.83361190467393 27.307927471024207 29.010486844170973 21.84797378013089 44-45 22.034360562841417 27.357635862712925 28.898697507854216 21.70930606659144 46-47 22.17004603862896 27.38195335923093 28.505647154260767 21.942353447879338 48-49 22.066062937744864 27.418783707180598 28.63989043425696 21.875262920817576 50-51 21.94060281107528 27.64537399760385 28.55018613478989 21.863837056530976 52-53 22.09711055122927 27.77340213220273 28.20005685829317 21.929430458274833 54-55 22.05703088584621 27.5912064864192 28.261320566260036 22.090442061474555 56-57 21.985292924643257 27.832997099765944 28.142072161115117 22.03963781447568 58-59 22.121419293202432 27.62572068075166 28.247926718327122 22.004933307718787 60-61 22.14438283955972 27.6359163575182 28.365900241555508 21.85380056136657 62-63 21.98043620143853 27.576972863619787 28.435432892980366 22.00715804196132 64-65 21.929411402768253 27.6903854131189 28.30049619780096 22.079706986311884 66-67 21.957240596267187 27.764181997105453 28.019424083288115 22.259153323339252 68-69 21.965631050429863 27.631224314410012 28.257426040548538 22.145718594611587 70-71 21.991219986445206 27.390645722122088 28.26710866056885 22.351025630863855 72-73 21.99772101710274 27.390812116374974 28.23704298874909 22.374423877773193 74-75 21.788182811517345 27.875341699588112 27.882217935867637 22.454257553026906 76-77 21.81213482664832 27.607176491314306 28.020385044520552 22.56030363751682 78-79 21.90987955556262 27.682529910354674 27.940081422748346 22.46750911133436 80-81 21.848565443389926 27.584583470731754 27.876830117666586 22.690020968211737 82-83 21.88162619981528 27.529977712129984 28.00559614071721 22.582799947337527 84-85 21.933211895144836 27.532926116256885 27.795241299865808 22.73862068873247 86-87 21.62990266793849 27.504950399000737 28.063907690007262 22.80123924305351 88-89 21.8121023145997 27.388420612117304 27.91248470810674 22.886992365176265 90-91 21.70820981214522 27.38362305553428 28.050110663166205 22.858056469154295 92-93 21.747753301209404 27.369262340934963 28.053109673284833 22.829874684570797 94-95 21.670825550239954 27.309067091171148 27.930799969550783 23.08930738903811 96-97 21.579731813500498 27.20525207208094 28.029699798875185 23.18531631554338 98-99 21.546721442911267 27.259193695197116 27.732130907279533 23.46195395461209 100-101 21.63092671692353 27.15052287669215 27.90389044360832 23.314659962776 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3689.0 1 2388.0 2 1048.0 3 882.5 4 725.0 5 684.0 6 882.5 7 1906.5 8 2645.0 9 2418.5 10 2147.5 11 2075.0 12 2142.0 13 2304.5 14 2565.0 15 2758.5 16 3048.0 17 3369.5 18 3779.5 19 4355.0 20 5086.0 21 6112.0 22 7518.5 23 9664.5 24 12205.5 25 15912.5 26 21233.0 27 26876.0 28 31809.5 29 36775.0 30 42246.0 31 48646.0 32 55601.5 33 62633.0 34 70086.0 35 77442.0 36 84456.0 37 89080.0 38 91493.0 39 94048.0 40 96907.5 41 99584.5 42 102274.5 43 105297.0 44 107658.5 45 108364.5 46 109305.5 47 112985.0 48 115161.5 49 111845.0 50 106405.0 51 101383.5 52 97902.5 53 94514.5 54 89011.0 55 81484.0 56 73565.0 57 66430.0 58 59421.0 59 51932.0 60 43512.5 61 34754.0 62 26577.0 63 19620.0 64 14224.5 65 10081.5 66 7001.0 67 4993.0 68 3588.5 69 2516.0 70 1706.5 71 1082.5 72 674.5 73 441.5 74 298.5 75 198.5 76 135.0 77 92.0 78 59.0 79 36.5 80 28.5 81 20.0 82 18.0 83 20.5 84 12.5 85 4.5 86 5.0 87 7.0 88 6.5 89 5.0 90 5.5 91 5.0 92 3.0 93 4.0 94 4.5 95 2.5 96 4.0 97 4.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.77942685795211 2 0.08874926946234098 3 0.12109151922127606 4 0.19408687548834627 5 0.12292725063174194 6 0.1486942442477356 7 0.15727211647482162 8 0.15350052284968263 9 0.14141807247534363 10-11 0.1298696530567765 12-13 0.1414848263448151 14-15 0.14884444045404646 16-17 0.14373776943947777 18-19 0.15049659872346577 20-21 0.17829958535833978 22-23 0.1972910612229776 24-25 0.22400929747893997 26-27 0.21628253708761547 28-29 0.21581526000131507 30-31 0.22958324557980908 32-33 0.22115556955903398 34-35 0.20590231038479934 36-37 0.19328582905468844 38-39 0.1891971545495599 40-41 0.19064905121056475 42-43 0.1843908759476129 44-45 0.19916016956817925 46-47 0.2250773593904838 48-49 0.22781426803881472 50-51 0.23757702144901957 52-53 0.2653633196165257 54-55 0.252329626602218 56-57 0.22828154512511512 58-59 0.18666050750964344 60-61 0.1961896223766981 62-63 0.20084570477233427 64-65 0.1882626003769591 66-67 0.19954400431764027 68-69 0.18661044210753983 70-71 0.20007803527341217 72-73 0.2199373114411793 74-75 0.2512448762233064 76-77 0.250911106875949 78-79 0.2456041742529658 80-81 0.250644091398063 82-83 0.24149881128046938 84-85 0.21217717411511905 86-87 0.22280772782845323 88-89 0.22536106333573758 90-91 0.21287808974456965 92-93 0.2104582619762283 94-95 0.2502769451159698 96-97 0.2798322208244704 98-99 0.29698796527864235 100-101 0.29241532521984553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2996081.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.50723199232827 #Duplication Level Percentage of deduplicated Percentage of total 1 83.45406099716874 61.34477022420797 2 10.478971949225148 15.405604442255866 3 3.027183196581143 6.675595725431038 4 1.2039023538944629 3.5398211849532144 5 0.6259404955260488 2.300557660901309 6 0.3378494641124445 1.4900627362198346 7 0.2135071672480706 1.098602461245013 8 0.13902756706694566 0.8175625300575162 9 0.10383042511911478 0.6869058432383555 >10 0.395161344693768 4.725834711823794 >50 0.013857665781315793 0.688660835795888 >100 0.006315334729973984 0.7912347324389819 >500 1.960194265099802E-4 0.08180622009203786 >1k 1.9601942634104357E-4 0.3529806913392101 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3961 0.13220603848827853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.3376934735743127E-5 0.0 4 0.0 0.0 0.0 3.3376934735743127E-5 3.3376934735743127E-5 5 0.0 0.0 0.0 3.3376934735743127E-5 3.3376934735743127E-5 6 0.0 0.0 0.0 3.3376934735743127E-5 3.3376934735743127E-5 7 0.0 0.0 0.0 3.3376934735743127E-5 3.3376934735743127E-5 8 0.0 0.0 0.0 3.3376934735743127E-5 3.3376934735743127E-5 9 0.0 0.0 0.0 3.3376934735743127E-5 3.3376934735743127E-5 10-11 0.0 0.0 0.0 5.006540210361469E-5 3.3376934735743127E-5 12-13 0.0 0.0 0.0 6.675386947148625E-5 3.3376934735743127E-5 14-15 0.0 0.0 0.0 1.0013080420722938E-4 3.3376934735743127E-5 16-17 0.0 0.0 0.0 1.0013080420722938E-4 3.3376934735743127E-5 18-19 0.0 0.0 0.0 1.335077389429725E-4 1.0013080420722938E-4 20-21 0.0 0.0 0.0 2.1695007578233032E-4 1.0013080420722938E-4 22-23 0.0 3.3376934735743127E-5 0.0 5.006540210361469E-4 1.335077389429725E-4 24-25 0.0 3.3376934735743127E-5 0.0 9.17865705232936E-4 1.835731410465872E-4 26-27 0.0 3.3376934735743127E-5 0.0 0.0014018312589012113 2.0026160841445876E-4 28-29 0.0 3.3376934735743127E-5 0.0 0.002953858724113267 2.0026160841445876E-4 30-31 0.0 3.3376934735743127E-5 0.0 0.00801046433657835 2.0026160841445876E-4 32-33 0.0 3.3376934735743127E-5 0.0 0.020576880264585637 2.0026160841445876E-4 34-35 0.0 3.3376934735743127E-5 0.0 0.03763249391455037 2.0026160841445876E-4 36-37 0.0 3.3376934735743127E-5 0.0 0.06376663381263725 2.0026160841445876E-4 38-39 0.0 3.3376934735743127E-5 0.0 0.10183302787875227 2.1695007578233032E-4 40-41 0.0 3.3376934735743127E-5 0.0 0.14632448188149788 2.3363854315020189E-4 42-43 0.0 3.3376934735743127E-5 3.3376934735743127E-5 0.19351946759783864 2.3363854315020189E-4 44-45 0.0 3.3376934735743127E-5 3.3376934735743127E-5 0.24995986423598027 2.5032701051807345E-4 46-47 3.3376934735743127E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.31799874569479264 2.67015477885945E-4 48-49 3.3376934735743127E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.38957558223559374 3.0039241262168814E-4 50-51 3.3376934735743127E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.4690293753740303 3.3376934735743127E-4 52-53 3.3376934735743127E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.5488002493924564 4.005232168289175E-4 54-55 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.637716403528476 4.3390015156466065E-4 56-57 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.7463249491585842 4.3390015156466065E-4 58-59 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.8662983410662128 4.3390015156466065E-4 60-61 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 0.9953335707545957 4.3390015156466065E-4 62-63 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 1.1282739018070607 4.3390015156466065E-4 64-65 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 1.2704763322486943 4.3390015156466065E-4 66-67 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 1.432120827173898 4.3390015156466065E-4 68-69 6.675386947148625E-5 3.3376934735743127E-5 3.3376934735743127E-5 1.6131740096479368 4.3390015156466065E-4 70-71 6.675386947148625E-5 5.006540210361469E-5 3.3376934735743127E-5 1.8163394113844051 4.3390015156466065E-4 72-73 6.675386947148625E-5 6.675386947148625E-5 3.3376934735743127E-5 2.0222083448344685 5.006540210361469E-4 74-75 6.675386947148625E-5 6.675386947148625E-5 3.3376934735743127E-5 2.236655150511618 5.3403095577189E-4 76-77 6.675386947148625E-5 6.675386947148625E-5 3.3376934735743127E-5 2.467056130992453 5.507194231397616E-4 78-79 6.675386947148625E-5 6.675386947148625E-5 3.3376934735743127E-5 2.735189736192045 5.674078905076332E-4 80-81 6.675386947148625E-5 6.675386947148625E-5 3.3376934735743127E-5 3.0324947823506774 6.174732926112478E-4 82-83 6.675386947148625E-5 1.0013080420722938E-4 3.3376934735743127E-5 3.336475215456458 7.676694989220919E-4 84-85 6.675386947148625E-5 1.0013080420722938E-4 3.3376934735743127E-5 3.6490502092566923 7.676694989220919E-4 86-87 6.675386947148625E-5 1.0013080420722938E-4 3.3376934735743127E-5 3.9849056150351077 7.676694989220919E-4 88-89 6.675386947148625E-5 1.0013080420722938E-4 6.675386947148625E-5 4.34587716420217 7.676694989220919E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11540 0.0 35.738014 1 GGTATCA 8265 0.0 28.0446 1 GTACATG 9490 0.0 27.6151 1 TATCAAC 15320 0.0 25.987658 2 TACATGG 10145 0.0 25.464409 2 ATCAACG 16030 0.0 24.837029 3 TCAACGC 16145 0.0 24.569479 4 CAACGCA 16165 0.0 24.315067 5 ACATGGG 10055 0.0 24.283655 3 AACGCAG 16615 0.0 23.713316 6 ACGCAGA 17410 0.0 22.251049 7 CATGGGG 5000 0.0 21.155958 4 GAGTACT 7855 0.0 21.055176 12-13 CGCAGAG 18480 0.0 20.732214 8 AGAGTAC 13515 0.0 19.848007 10-11 GTATAGG 790 3.6379788E-12 19.824549 1 AGTACTT 8790 0.0 19.406017 12-13 GCAGAGT 18445 0.0 19.134705 9 CATGGGA 5945 0.0 18.587402 4 GTACTTT 8840 0.0 18.551905 14-15 >>END_MODULE