FastQCFastQC Report
Fri 27 May 2016
SRR939692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996081
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97610.32579225995558864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74700.24932570247600114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55090.18387353345920887No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31560.1053376060260053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91450.029.3507331
GTATCAA131050.027.5368821
TACATGG102450.023.5677322
GTACATG107450.023.3119451
ATCAACG155550.022.7940033
TCAACGC155900.022.7730714
ACATGGG100900.022.5231253
CAACGCA158350.022.3909515
TATCAAC160400.022.2268162
AACGCAG161400.022.0137526
ACGCAGA180200.019.481747
CGCAGAG179200.019.2749048
GAGTACT89050.019.15614512-13
CATGGGA61750.019.0123214
AGAGTAC143500.018.40590910-11
CATGGGG46000.017.9371324
GCAGAGT189000.017.477929
AGTACTT94800.017.372912-13
GTACTTT97050.017.35857414-15
ATTATAC16200.016.2984313
Produced by FastQC (version 0.11.5)