##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939692_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2996081 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47786458376793 31.0 31.0 34.0 30.0 34.0 2 31.68147523381377 31.0 31.0 34.0 30.0 34.0 3 31.737176998886213 31.0 31.0 34.0 30.0 34.0 4 35.54307143231441 37.0 35.0 37.0 33.0 37.0 5 35.296526362271244 37.0 35.0 37.0 33.0 37.0 6 35.3000880149769 37.0 35.0 37.0 32.0 37.0 7 35.20516534766583 37.0 35.0 37.0 32.0 37.0 8 35.16360472230223 37.0 35.0 37.0 32.0 37.0 9 36.766738616212315 39.0 35.0 39.0 32.0 39.0 10-11 36.65022908259156 39.0 35.0 39.0 32.0 39.0 12-13 36.58865781666117 39.0 35.0 39.0 32.0 39.0 14-15 37.664424793588694 39.0 36.5 41.0 32.0 41.0 16-17 37.61662601912298 39.0 36.0 41.0 32.0 41.0 18-19 37.5879582361091 39.0 36.0 41.0 32.0 41.0 20-21 37.52420779010981 39.0 36.0 41.0 32.0 41.0 22-23 37.3927045029824 39.0 36.0 41.0 32.0 41.0 24-25 37.269598518865145 39.0 36.0 41.0 31.0 41.0 26-27 36.98922559169795 39.0 36.0 40.0 31.0 41.0 28-29 36.79516074498653 39.0 36.0 40.0 30.0 41.0 30-31 36.55995782490527 38.0 35.0 40.0 30.0 41.0 32-33 36.39310569373792 38.0 35.0 40.0 30.0 41.0 34-35 36.367287299642435 38.0 35.0 40.0 30.0 41.0 36-37 36.30017279239112 38.0 35.0 40.0 30.0 41.0 38-39 36.344714645565325 38.0 35.0 40.0 30.0 41.0 40-41 36.24747411702154 38.0 35.0 40.0 30.0 41.0 42-43 36.07765344127879 38.0 35.0 40.0 29.5 41.0 44-45 35.87100031674711 38.0 35.0 40.0 29.0 41.0 46-47 35.59980137386139 38.0 34.5 40.0 27.5 41.0 48-49 35.31871301209814 38.0 34.0 40.0 27.0 41.0 50-51 35.092411052972196 38.0 34.0 40.0 26.5 41.0 52-53 34.882847125962215 38.0 34.0 40.0 26.0 41.0 54-55 34.61617576427339 38.0 33.0 40.0 26.0 41.0 56-57 33.95267517800754 37.5 32.5 40.0 23.5 41.0 58-59 30.957078597007225 35.5 28.0 39.0 8.5 40.5 60-61 30.729911173963586 35.0 27.0 39.0 10.5 40.0 62-63 32.40209009703008 36.0 31.0 39.0 20.0 40.0 64-65 32.43683915755281 36.0 31.0 39.0 19.0 40.0 66-67 27.677740521701516 32.0 19.0 37.0 5.0 39.5 68-69 19.98475274867402 20.5 8.0 32.5 2.0 37.0 70-71 18.015718700529124 16.0 7.5 29.0 2.0 35.5 72-73 19.791555702265725 20.0 7.5 31.0 2.0 36.0 74-75 21.32556129156722 24.5 9.5 32.5 2.0 36.0 76-77 23.281339690081808 27.5 12.5 33.5 2.0 36.0 78-79 25.56293020782816 31.0 18.0 35.0 2.0 37.0 80-81 27.16552890258975 32.5 24.0 35.0 2.0 37.0 82-83 28.14628559775253 33.5 27.5 35.0 2.0 37.0 84-85 28.13555574765836 34.0 28.0 35.0 2.0 36.0 86-87 27.838989166180752 34.0 27.5 35.0 2.0 36.0 88-89 27.390840901831425 33.0 26.5 35.0 2.0 35.5 90-91 25.671647562265505 32.5 19.0 34.5 2.0 35.0 92-93 24.197717952218248 31.5 6.5 34.0 2.0 35.0 94-95 20.307153077637086 29.0 2.0 34.0 2.0 35.0 96-97 17.54250602703999 19.5 2.0 33.0 2.0 35.0 98-99 17.835218907633006 24.0 2.0 33.0 2.0 35.0 100-101 17.040762082200047 20.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18523424295217694 1101 2 0.1416948769837134 1101 3 0.17175725419320642 1101 4 0.16477534605451893 1101 5 0.3011964696685041 1101 6 0.25217976662771235 1101 7 0.25606443529191125 1101 8 0.1948735752631947 1101 9 0.22114870895798333 1101 10-11 0.2415187306793527 1101 12-13 0.21816677529471207 1101 14-15 0.2513623287336415 1101 16-17 0.3074717884747713 1101 18-19 0.35831130737722106 1101 20-21 0.3670149373395546 1101 22-23 0.2538737040671464 1101 24-25 0.360836193938745 1101 26-27 0.4056945629002655 1101 28-29 0.35736994445704084 1101 30-31 0.06475108928463413 1101 32-33 0.20132241605283951 1101 34-35 0.055338495425800716 1101 36-37 0.05501922983447827 1101 38-39 0.05793000116144498 1101 40-41 0.142938364848618 1101 42-43 0.12467379370821163 1101 44-45 0.15253003148018962 1101 46-47 -0.020412804351899183 1101 48-49 -0.13804833729099641 1101 50-51 -0.10712572594312775 1101 52-53 -0.04288173301738141 1101 54-55 -0.0799552046858949 1101 56-57 -3.222771613392112 1101 58-59 -10.182547470555036 1101 60-61 -11.893013014766133 1101 62-63 -3.987226403170588 1101 64-65 -1.196013678960746 1101 66-67 -2.1392820377895063 1101 68-69 -4.820846963638498 1101 70-71 -4.614581737562197 1101 72-73 -3.6439210297617493 1101 74-75 -6.571818962875319 1101 76-77 -4.9804118492399105 1101 78-79 1.1135406152442684 1101 80-81 1.6659702788969604 1101 82-83 0.2672304212634238 1101 84-85 0.16632891956951923 1101 86-87 0.14847669264976204 1101 88-89 -0.07673650300495538 1101 90-91 1.2581605284004915 1101 92-93 0.8260996640876925 1101 94-95 -8.906428486460708 1101 96-97 -9.704591759102266 1101 98-99 -8.800435183284883 1101 100-101 -9.719959489633133 1102 1 0.2630530295954081 1102 2 0.2750206063632241 1102 3 0.32211641619909415 1102 4 0.18860375630779913 1102 5 0.3362850682118079 1102 6 0.2824626087475508 1102 7 0.3056856105367416 1102 8 0.20054860186263568 1102 9 0.21991786693723014 1102 10-11 0.27622737155517285 1102 12-13 0.2599154100873733 1102 14-15 0.3511996982111043 1102 16-17 0.34632463219028153 1102 18-19 0.3298364204908282 1102 20-21 0.26361115030172044 1102 22-23 0.25817157014039793 1102 24-25 0.2283511719036042 1102 26-27 -0.018107291120337266 1102 28-29 0.19543222659944348 1102 30-31 0.22454630674331355 1102 32-33 0.07038881181905055 1102 34-35 0.04625676309917992 1102 36-37 -0.14176197426697712 1102 38-39 -0.016381269760707085 1102 40-41 0.0039873417999984895 1102 42-43 0.12004234256467328 1102 44-45 0.028446658350915754 1102 46-47 0.004294573038656324 1102 48-49 0.1398550049347378 1102 50-51 0.1500533857652897 1102 52-53 -9.035394671812469E-4 1102 54-55 -0.10630693709124017 1102 56-57 -3.73215711983368 1102 58-59 -10.243328600153102 1102 60-61 -8.14518393398238 1102 62-63 -2.4763635022660395 1102 64-65 -0.7510236284270952 1102 66-67 -2.0249026346730012 1102 68-69 -5.240091581872971 1102 70-71 -4.958960149860481 1102 72-73 -3.70866576829615 1102 74-75 -4.282486311593601 1102 76-77 -4.129959826828216 1102 78-79 1.7238505109260878 1102 80-81 2.150985593375175 1102 82-83 0.8282780732834532 1102 84-85 0.3941570095350464 1102 86-87 0.2753151549075561 1102 88-89 0.273825306290572 1102 90-91 1.5791551985281096 1102 92-93 1.0407517438362746 1102 94-95 -9.05568526718092 1102 96-97 -10.02007442679201 1102 98-99 -9.31791592425562 1102 100-101 -10.094043198795724 1103 1 0.16316981094859173 1103 2 0.11643318231499578 1103 3 0.22837678297113229 1103 4 0.14213804349331127 1103 5 0.1308245761397231 1103 6 0.14746026169409987 1103 7 0.20668621799300269 1103 8 0.2929314681959667 1103 9 0.2468330379321273 1103 10-11 0.28610622309403766 1103 12-13 0.2707864061270655 1103 14-15 0.3365194897009758 1103 16-17 0.3504022717707542 1103 18-19 0.3290566859526578 1103 20-21 0.24383031576513048 1103 22-23 0.3148441088533076 1103 24-25 0.4158145528998176 1103 26-27 0.32422260045628803 1103 28-29 0.332443341126087 1103 30-31 0.26095968207742715 1103 32-33 0.17529109255303155 1103 34-35 0.1412854874475329 1103 36-37 0.04785597671124009 1103 38-39 0.02820039037387545 1103 40-41 0.09846625273746668 1103 42-43 0.10709866562327619 1103 44-45 -0.10145469108637428 1103 46-47 -0.012131410333999781 1103 48-49 -0.12322478995889696 1103 50-51 -0.23019003521584125 1103 52-53 -0.06105657311157131 1103 54-55 -0.016997489172275948 1103 56-57 0.2581416485043846 1103 58-59 -8.818463666041993 1103 60-61 -8.026259576546902 1103 62-63 -2.604886997093189 1103 64-65 -0.6550263555239084 1103 66-67 -2.0329096495433703 1103 68-69 -3.552695597958717 1103 70-71 -4.058096937696046 1103 72-73 -3.2568275094019885 1103 74-75 -3.851517227020622 1103 76-77 -0.5807492737921898 1103 78-79 3.128100205827721 1103 80-81 2.560328110919553 1103 82-83 0.9768963030468427 1103 84-85 0.4932939367167499 1103 86-87 0.3886859649201533 1103 88-89 0.29930052074660196 1103 90-91 1.7573611653111492 1103 92-93 1.202321732617051 1103 94-95 -7.445185266298395 1103 96-97 -8.941437313769008 1103 98-99 -8.26897254421439 1103 100-101 -9.796417127467782 1104 1 0.18950961725916216 1104 2 0.1478278822296879 1104 3 0.17523725379420085 1104 4 0.10590688985122654 1104 5 0.26737271800308804 1104 6 0.12927338915672948 1104 7 0.18546768405692404 1104 8 0.2292612976589794 1104 9 0.24218943775158408 1104 10-11 0.16977460531956723 1104 12-13 0.2864364532432475 1104 14-15 0.32473202232240794 1104 16-17 0.29075188666006824 1104 18-19 0.2790483408027242 1104 20-21 0.23778028693271125 1104 22-23 0.3175119617787132 1104 24-25 0.31894252270102896 1104 26-27 0.31925402657002167 1104 28-29 0.3292845220417462 1104 30-31 0.3272607373906169 1104 32-33 0.26901099617724356 1104 34-35 0.21952493306369547 1104 36-37 0.1515000073066659 1104 38-39 0.10400367137906841 1104 40-41 0.10497729078033302 1104 42-43 0.14166852891487025 1104 44-45 0.13894344677513715 1104 46-47 0.012277265453242592 1104 48-49 -0.10071564876450623 1104 50-51 0.04296657215337518 1104 52-53 -0.04793766050691062 1104 54-55 -0.002192929123758347 1104 56-57 0.4254430564718561 1104 58-59 -8.493654541428644 1104 60-61 -7.984764832261327 1104 62-63 -2.4541730884097994 1104 64-65 -0.5907174828129875 1104 66-67 -1.6641606471598358 1104 68-69 -3.452563678119869 1104 70-71 -4.152937722888609 1104 72-73 -3.0168473574738215 1104 74-75 -3.8603679573737573 1104 76-77 -0.4859764608247126 1104 78-79 3.4507761498608573 1104 80-81 2.899233391145863 1104 82-83 1.3535174724841603 1104 84-85 0.9228282281807445 1104 86-87 0.9569926789096925 1104 88-89 0.8749913949040184 1104 90-91 2.3739479634751603 1104 92-93 2.1778167211109434 1104 94-95 -6.451761362820594 1104 96-97 -8.060288183435613 1104 98-99 -5.991803071565039 1104 100-101 -5.414011626388648 1105 1 0.23900453267388855 1105 2 0.22120840359082194 1105 3 0.27668770427897016 1105 4 0.17162792759602752 1105 5 0.21807497867131787 1105 6 0.2910167304732454 1105 7 0.25774323935610965 1105 8 0.22853336056522267 1105 9 0.3084731619815173 1105 10-11 0.19685829123892518 1105 12-13 0.15137899918009623 1105 14-15 0.30012587111791333 1105 16-17 0.3913713678384596 1105 18-19 0.2933931729300454 1105 20-21 0.2723263257341344 1105 22-23 0.4214186330963372 1105 24-25 0.34636777491681414 1105 26-27 0.20540388510933383 1105 28-29 0.32188066896350875 1105 30-31 0.43975926129091647 1105 32-33 0.30471265552191795 1105 34-35 0.28420984405057936 1105 36-37 0.2791208665409002 1105 38-39 0.35940098118622643 1105 40-41 0.3294050311877754 1105 42-43 0.20381169144873468 1105 44-45 0.15409820641894356 1105 46-47 0.19829521373723225 1105 48-49 0.22497797297920386 1105 50-51 0.30576229319655823 1105 52-53 0.3941556511243576 1105 54-55 0.22821294705002515 1105 56-57 0.7964807831313934 1105 58-59 -8.461170371294163 1105 60-61 -7.843843611815746 1105 62-63 -2.24641910720338 1105 64-65 -0.271282297058427 1105 66-67 -1.659067173543061 1105 68-69 -3.3246269652936817 1105 70-71 -3.851780596769018 1105 72-73 -2.9505640867307434 1105 74-75 -3.7774294750418775 1105 76-77 -0.3374165718092712 1105 78-79 3.711163402236629 1105 80-81 3.0538461959094327 1105 82-83 1.5086583173374244 1105 84-85 1.1554937839860173 1105 86-87 1.1110390785959545 1105 88-89 1.058401839141549 1105 90-91 2.4414112977027678 1105 92-93 2.091273426449156 1105 94-95 -6.518567685297995 1105 96-97 -7.693159830875345 1105 98-99 -5.548447071144928 1105 100-101 -5.053996623229658 1106 1 0.06272523814417497 1106 2 0.11073092921217054 1106 3 0.14933616407382289 1106 4 0.1488875537182679 1106 5 0.1452248890966672 1106 6 0.1364120889674112 1106 7 0.19988854219738528 1106 8 0.17982017600269273 1106 9 0.17383008799735222 1106 10-11 0.10258649970570133 1106 12-13 0.1524032316728352 1106 14-15 0.1937130681386492 1106 16-17 0.31466103299631243 1106 18-19 0.2002026375401087 1106 20-21 0.15833284982461038 1106 22-23 0.21058766691466246 1106 24-25 0.20798837244424817 1106 26-27 0.14936901002951686 1106 28-29 0.32299674243189713 1106 30-31 0.35200105107322344 1106 32-33 0.2941212435030067 1106 34-35 0.36204100433199926 1106 36-37 0.3381674859339867 1106 38-39 0.4619025465950912 1106 40-41 0.41784917012516587 1106 42-43 0.36142021823906845 1106 44-45 0.4110179898606674 1106 46-47 0.2699319972085661 1106 48-49 0.22285786289431542 1106 50-51 0.20881304022465486 1106 52-53 0.33056093867890723 1106 54-55 0.2561266636870769 1106 56-57 0.9215870170957814 1106 58-59 -8.38864094836985 1106 60-61 -7.530126079199864 1106 62-63 -1.8408054943420638 1106 64-65 0.03224308047652613 1106 66-67 0.8949059858632609 1106 68-69 -2.043567809470346 1106 70-71 -2.450732348546527 1106 72-73 -1.0389069721347717 1106 74-75 -0.9943670893404857 1106 76-77 2.276386368344774 1106 78-79 4.587350344039969 1106 80-81 3.5463393612959315 1106 82-83 1.7363954251490412 1106 84-85 1.2902941299294959 1106 86-87 1.1533944916573624 1106 88-89 1.0911204380265893 1106 90-91 2.3904027284371274 1106 92-93 2.0019649771159465 1106 94-95 -6.245031264913795 1106 96-97 -7.757439466815889 1106 98-99 -5.722291711671126 1106 100-101 -5.152288125207027 1107 1 -0.032347546958686024 1107 2 -0.01874773618480674 1107 3 0.004636631516309109 1107 4 0.005382818796185518 1107 5 -0.007344421698398662 1107 6 -0.05257005921237834 1107 7 -0.05555363841468619 1107 8 -0.05467860494668031 1107 9 -0.07356974852490339 1107 10-11 -0.06488018489675795 1107 12-13 -0.003929890025396787 1107 14-15 0.07893847394338849 1107 16-17 -0.00476863776354719 1107 18-19 -0.007088576261402579 1107 20-21 0.03643743482898287 1107 22-23 0.011099918819688526 1107 24-25 -0.016766208079175726 1107 26-27 9.834744740047086E-4 1107 28-29 0.010420478513253784 1107 30-31 -0.11150866427606587 1107 32-33 -0.06008396272039107 1107 34-35 -0.00869558678736837 1107 36-37 0.04988009112117453 1107 38-39 0.18312809232784133 1107 40-41 0.0016930483380335204 1107 42-43 0.03092911241292029 1107 44-45 -0.028153719293207757 1107 46-47 -0.01253166072949341 1107 48-49 -0.02561676857300199 1107 50-51 -0.08934067645972021 1107 52-53 -0.02190224501861593 1107 54-55 -0.07069047547403073 1107 56-57 0.500000300230532 1107 58-59 -8.671715348775646 1107 60-61 -7.820096171391864 1107 62-63 -2.2741834898337103 1107 64-65 -0.4985532072479124 1107 66-67 0.3706447507353481 1107 68-69 -2.6753161576076145 1107 70-71 -2.5558532176725492 1107 72-73 -1.19184697493694 1107 74-75 -1.0634952657377674 1107 76-77 2.0519359418979484 1107 78-79 4.064762745231594 1107 80-81 2.864713531307377 1107 82-83 1.240586052533203 1107 84-85 0.8946898888847521 1107 86-87 0.8166163628374079 1107 88-89 0.6143297787979876 1107 90-91 1.959084298277883 1107 92-93 1.468911674102749 1107 94-95 -6.914017142102807 1107 96-97 -8.379058136313333 1107 98-99 -6.24303437054486 1107 100-101 -5.337944368619727 1108 1 -0.006158914570502105 1108 2 -0.06892830234709635 1108 3 -0.08608564652299933 1108 4 -0.13019958351263483 1108 5 -0.015294064025198395 1108 6 -0.08922827074371042 1108 7 -0.024846455536938095 1108 8 -0.058726268521859026 1108 9 0.05496041925812989 1108 10-11 -0.10891395842380547 1108 12-13 -0.03555309301157905 1108 14-15 -0.03951989139611811 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0.3361341667499964 2312 84-85 0.02963753268172198 2312 86-87 -0.017181645999222894 2312 88-89 0.03338116432104954 2312 90-91 1.7441598502890763 2312 92-93 3.3489061894653034 2312 94-95 5.436458146770995 2312 96-97 0.2110129048704863 2312 98-99 -1.190341290091455 2312 100-101 -0.7559913820833462 2313 1 -0.2570115857099786 2313 2 -0.2887424410792576 2313 3 -0.3174755299723486 2313 4 -0.24537731467054158 2313 5 -0.2987900729203403 2313 6 -0.27811577062266224 2313 7 -0.24687284692139855 2313 8 -0.3546951073555249 2313 9 -0.4897649573371936 2313 10-11 -0.4176880505321847 2313 12-13 -0.32144213615882933 2313 14-15 -0.5047648379187493 2313 16-17 -0.5024588249662401 2313 18-19 -0.565096903334954 2313 20-21 -0.4763183678043035 2313 22-23 -0.4350827803275905 2313 24-25 -0.35970365783117586 2313 26-27 -0.5277998120121765 2313 28-29 -0.4919049364025838 2313 30-31 -0.56362838470708 2313 32-33 -0.4999579867576287 2313 34-35 -0.5060588287555134 2313 36-37 -0.5641806167657464 2313 38-39 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3.2208977758064243 2314 62-63 0.628921477656263 2314 64-65 -0.24504690694368492 2314 66-67 -2.2481075723532022 2314 68-69 -4.87812262473382 2314 70-71 -3.617738358889909 2314 72-73 -2.77404185542229 2314 74-75 -4.116762607208443 2314 76-77 0.3567327784458776 2314 78-79 3.0970565826832157 2314 80-81 2.283678037156715 2314 82-83 0.5820507389448757 2314 84-85 0.3218076648500592 2314 86-87 0.37133000859348897 2314 88-89 0.40257270471810713 2314 90-91 2.081164737475941 2314 92-93 3.810113884538019 2314 94-95 6.354451566004528 2314 96-97 3.3675302485081566 2314 98-99 5.763296276960471 2314 100-101 6.3486134263002185 2315 1 -0.44526760450034786 2315 2 -0.4293531281021572 2315 3 -0.45748727395355715 2315 4 -0.3089944608831061 2315 5 -0.49583058965551174 2315 6 -0.5296366161893076 2315 7 -0.4700789537916279 2315 8 -0.5321584074142507 2315 9 -0.685854211594382 2315 10-11 -0.5048753670324828 2315 12-13 -0.5489289241799682 2315 14-15 -0.6330326006903277 2315 16-17 -0.6757177797519205 2315 18-19 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1.276585270766546 2315 84-85 0.8665578794035618 2315 86-87 0.7686076634107799 2315 88-89 1.028209364007143 2315 90-91 2.6096102408862087 2315 92-93 4.3078265183516535 2315 94-95 7.022105931603697 2315 96-97 4.72174340176711 2315 98-99 6.977090712120148 2315 100-101 7.167319329909233 2316 1 -0.5054221466604325 2316 2 -0.455745001195222 2316 3 -0.5726288986335426 2316 4 -0.35076479860799736 2316 5 -0.5355748118595471 2316 6 -0.5608648873511797 2316 7 -0.45608458838475485 2316 8 -0.5472628578076169 2316 9 -0.5886932516093495 2316 10-11 -0.6973986594117534 2316 12-13 -0.5901799908949528 2316 14-15 -0.6885387204195865 2316 16-17 -0.7426658643409496 2316 18-19 -0.7324629333747268 2316 20-21 -0.6925388201364129 2316 22-23 -0.865501445164611 2316 24-25 -0.6466008458400836 2316 26-27 -0.713083371607496 2316 28-29 -0.6087707231998607 2316 30-31 -0.748770674819383 2316 32-33 -0.5942579744381291 2316 34-35 -0.6485000571435791 2316 36-37 -0.5194976907699029 2316 38-39 -0.6757943153930412 2316 40-41 -0.5930149588124181 2316 42-43 -0.6425832953398825 2316 44-45 -0.7583161090148565 2316 46-47 -0.5903676680367838 2316 48-49 -0.5852575954617052 2316 50-51 -0.5391252829225692 2316 52-53 -0.39409083392537525 2316 54-55 -0.3961136602893873 2316 56-57 0.44678549071100093 2316 58-59 3.5625819403522847 2316 60-61 3.655553257561099 2316 62-63 0.7888660212737975 2316 64-65 0.16255637150715785 2316 66-67 -1.967611092746111 2316 68-69 -3.0453566923507367 2316 70-71 -2.9733016670624774 2316 72-73 -0.36514300895128926 2316 74-75 5.884271083450056 2316 76-77 6.042396805195427 2316 78-79 5.011107118670964 2316 80-81 3.2843313713357887 2316 82-83 1.5364124921639721 2316 84-85 1.095282614332195 2316 86-87 1.0672672848876985 2316 88-89 0.9698312383861101 2316 90-91 2.4924555935158814 2316 92-93 4.164131300741797 2316 94-95 6.67573159355149 2316 96-97 3.5911225444205144 2316 98-99 6.10620542635743 2316 100-101 6.387992441677582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 39.0 5 87.0 6 257.0 7 654.0 8 1434.0 9 2535.0 10 4237.0 11 6460.0 12 9488.0 13 12675.0 14 15979.0 15 21450.0 16 28425.0 17 36883.0 18 39920.0 19 39549.0 20 41727.0 21 42843.0 22 43589.0 23 44591.0 24 47463.0 25 51527.0 26 65184.0 27 91519.0 28 126467.0 29 166803.0 30 205062.0 31 236176.0 32 250437.0 33 252057.0 34 257147.0 35 273663.0 36 276698.0 37 211770.0 38 85436.0 39 5839.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.613680470954776 20.202572249578253 14.501229769400293 23.68251751006668 2 15.168908960842442 21.4269142731819 40.16838320147066 23.235793564505 3 18.63731093603767 25.734093527667522 30.80652827231352 24.82206726398129 4 12.52105667370141 17.70936767063374 38.47616269386575 31.293412961799095 5 12.74177809214468 38.41114916019287 35.384811136069 13.46226161159346 6 30.557585058614904 38.153674750449 17.870678396211584 13.418061794724508 7 26.823874254400998 33.3530034735376 22.176169469383506 17.646952802677898 8 25.04928109348288 36.243315427702484 20.44292571822229 18.26447776059235 9 25.734651366234758 16.54728293393937 20.82210060408914 36.895965095736734 10-11 24.04077192839579 27.075452899971665 28.722854956191103 20.16092021544144 12-13 25.06714604845463 24.49292926326091 28.772403015806315 21.667521672478145 14-15 22.10829871848575 25.885619971529323 27.02586559370374 24.98021571628119 16-17 21.450779017916744 28.85286774718966 27.648777736242025 22.04757549865157 18-19 21.30156955349916 27.90979865323799 29.58186961274334 21.20676218051951 20-21 22.355653935924963 27.08821957750808 29.33235116140051 21.223775325166443 22-23 21.75093063238277 27.099534358383504 29.27736266142337 21.872172347810356 24-25 21.670411564993678 27.106471931953664 29.543008922337084 21.68010758071558 26-27 21.73984658926205 27.232333508395733 29.425152812455096 21.602667089887117 28-29 21.528094282947304 27.61563534332172 29.29096036759121 21.56531000613976 30-31 21.821226266228138 27.304658626052703 29.223554536167658 21.650560571551498 32-33 21.70941021101932 27.31525190755865 29.19533143596913 21.780006445452894 34-35 21.914789826688146 27.34620364948045 29.207025130544217 21.531981393287182 36-37 22.00896178214799 27.06549737256234 29.32915909635252 21.596381748937148 38-39 21.666946134426443 27.47517691645295 29.274921453971658 21.58295549514895 40-41 21.961499031780384 27.3588933067748 29.13990297427312 21.5397046871717 42-43 21.663645439238252 27.367309712362335 29.04405335421385 21.924991494185562 44-45 21.877106147231498 27.40664302743021 28.943271344505096 21.772979480833193 46-47 22.012153386142096 27.448590628777893 28.559956359783257 21.97929962529676 48-49 21.868955878003366 27.48695292162868 28.69634097277787 21.947750227590078 50-51 21.78461954674765 27.688306136132685 28.625672275889247 21.901402041230416 52-53 21.994010321216773 27.788068566543128 28.24094305296616 21.97697805927394 54-55 21.99788755416774 27.56882062008934 28.32674321026984 22.10654861547308 56-57 21.852608979479232 27.773236310900863 28.3516902300606 22.022464479559307 58-59 21.129215032213807 27.37682757878596 29.75557184553513 21.738385543465103 60-61 21.329687439031108 27.59818953481187 29.299113775046333 21.773009251110693 62-63 21.919676473572622 27.54939612494593 28.525219093292886 22.005708308188563 64-65 21.805756539887856 27.715399621197268 28.354497811011512 22.124346027903368 66-67 21.847375417839476 27.357100756111375 28.858807897478343 21.936715928570802 68-69 21.364963085414526 26.75395379027658 30.327070154388743 21.55401296992015 70-71 22.233209015196543 26.68802394349532 29.314503911815056 21.76426312949308 72-73 21.456192710313555 26.93507106522684 29.620481014018523 21.988255210441082 74-75 21.43734686322964 27.28578692916099 29.193277734401885 22.08358847320748 76-77 20.27340432866365 27.638707420878543 29.450046831358346 22.63784141909946 78-79 21.61688365204836 27.54406498835693 28.475712033377082 22.36333932621763 80-81 21.63441431806271 27.64944714194868 28.077532521282812 22.6386060187058 82-83 21.84689240192839 27.671861461458253 27.95599978104694 22.525246355566413 84-85 21.918187715357078 27.586467009886213 27.791170449407993 22.704174825348712 86-87 21.58478964552519 27.604105822422813 28.041516829893347 22.76958770215865 88-89 21.80353418643737 27.42618392675626 27.877323641553453 22.89295824525292 90-91 21.603856237081015 27.44921491428426 28.22246171694046 22.72446713169426 92-93 21.554094964121038 27.513072018749835 28.100724258467462 22.83210875866166 94-95 20.509189700255074 27.48555880169443 28.912631487584573 23.092620010465918 96-97 20.270450593669135 27.481657187269004 29.008518725135048 23.239373493926813 98-99 20.94416380261936 27.288296661936702 28.413561095298817 23.35397844014512 100-101 20.250096188259057 27.382228975731017 28.924078086736632 23.443596749273297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 609.0 1 469.5 2 345.0 3 405.5 4 434.0 5 481.0 6 648.0 7 965.5 8 1419.0 9 1752.0 10 1905.5 11 2056.0 12 2220.0 13 2388.5 14 2559.0 15 2823.0 16 3188.5 17 3571.5 18 4029.5 19 4554.5 20 5228.5 21 6170.5 22 7563.0 23 9335.5 24 11728.5 25 15226.0 26 19880.5 27 25203.5 28 30342.0 29 35797.5 30 41774.5 31 48580.0 32 55625.5 33 62726.0 34 70713.0 35 78142.5 36 85032.0 37 90121.0 38 93657.5 39 96585.0 40 99781.0 41 103049.0 42 105175.5 43 107908.0 44 110538.0 45 112230.0 46 113562.0 47 115112.5 48 116395.0 49 114028.0 50 108814.5 51 104128.0 52 99599.5 53 95321.5 54 89790.0 55 82216.5 56 73919.5 57 65361.0 58 57034.0 59 48584.5 60 39499.5 61 30895.5 62 23281.0 63 16923.5 64 12097.5 65 8370.5 66 5685.0 67 3841.5 68 2597.5 69 1718.5 70 1085.0 71 649.0 72 372.5 73 228.5 74 153.0 75 90.5 76 45.5 77 19.0 78 10.5 79 6.5 80 2.5 81 0.5 82 1.5 83 2.0 84 1.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026835055527537472 2 0.013818050980597655 3 1.6688467367871563E-4 4 0.0 5 0.05684091985497054 6 0.0 7 0.0 8 1.0013080420722938E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 5.3403095577189E-4 16-17 3.3376934735743127E-5 18-19 2.0026160841445876E-4 20-21 0.0 22-23 0.0 24-25 1.6688467367871563E-4 26-27 2.1695007578233032E-4 28-29 0.001184881183118881 30-31 0.005523882698765487 32-33 0.0056907673724442024 34-35 0.002186189225191175 36-37 0.08277479814464295 38-39 0.07660006521853048 40-41 0.0815064746246847 42-43 0.08479410269615542 44-45 0.08716386506239317 46-47 0.08384286005618673 48-49 0.07381309116809592 50-51 0.0685395354798485 52-53 0.058860224406483 54-55 0.04832980149735605 56-57 0.06894005869667742 58-59 0.7410013280682332 60-61 0.34843851017379035 62-63 0.003237562669367083 64-65 0.0020860584209839456 66-67 1.1287745558280968 68-69 2.429123244665281 70-71 2.3985666609147085 72-73 1.8538550860273804 74-75 1.314033232078839 76-77 0.7558540640256388 78-79 0.5764363513536517 80-81 0.04120382593127489 82-83 1.6688467367871563E-4 84-85 0.0013183889220618535 86-87 0.0014352081936369544 88-89 8.344233683935782E-5 90-91 0.3392765415888289 92-93 0.05700780452864926 94-95 0.9782278917025273 96-97 1.6996202706135115 98-99 0.6565076177846995 100-101 0.5856650738080846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2996081.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.39790024141405 #Duplication Level Percentage of deduplicated Percentage of total 1 71.91362660047186 37.681230326098245 2 14.639441981029341 15.341520410238887 3 5.50328559749276 8.650818292123091 4 2.7110949654593797 5.682227341805619 5 1.4883495728948477 3.899319622244772 6 0.9284958358665271 2.919073930938158 7 0.5911043055403928 2.1680837103783084 8 0.42954994500119237 1.8006012133501899 9 0.3100780749669891 1.4622696035253002 >10 1.3723385129895687 12.249558877201604 >50 0.07269228064499023 2.604477149783477 >100 0.03726737061829202 3.604980257827968 >500 0.001974373041660484 0.7425923203573331 >1k 5.095156234969494E-4 0.434369119162048 >5k 1.9106835881135603E-4 0.758877824965041 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9761 0.32579225995558864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7470 0.24932570247600114 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5509 0.18387353345920887 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3156 0.1053376060260053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.3376934735743127E-5 0.0 4 0.0 0.0 0.0 3.3376934735743127E-5 0.0 5 0.0 0.0 0.0 3.3376934735743127E-5 0.0 6 0.0 0.0 0.0 3.3376934735743127E-5 0.0 7 0.0 0.0 0.0 3.3376934735743127E-5 0.0 8 0.0 0.0 0.0 3.3376934735743127E-5 3.3376934735743127E-5 9 0.0 0.0 0.0 3.3376934735743127E-5 6.675386947148625E-5 10-11 0.0 0.0 0.0 5.006540210361469E-5 6.675386947148625E-5 12-13 0.0 0.0 0.0 6.675386947148625E-5 6.675386947148625E-5 14-15 0.0 0.0 0.0 1.0013080420722938E-4 8.344233683935782E-5 16-17 0.0 0.0 0.0 1.0013080420722938E-4 1.0013080420722938E-4 18-19 0.0 0.0 0.0 1.0013080420722938E-4 1.0013080420722938E-4 20-21 0.0 0.0 0.0 2.0026160841445876E-4 1.0013080420722938E-4 22-23 0.0 0.0 0.0 5.006540210361469E-4 1.335077389429725E-4 24-25 0.0 0.0 0.0 9.17865705232936E-4 2.0026160841445876E-4 26-27 0.0 0.0 0.0 0.0014352081936369544 2.0026160841445876E-4 28-29 0.0 0.0 0.0 0.003120743397791982 2.3363854315020189E-4 30-31 0.0 0.0 0.0 0.00852780682498237 2.67015477885945E-4 32-33 0.0 0.0 0.0 0.0215948767740258 3.0039241262168814E-4 34-35 0.0 0.0 0.0 0.03960173306395922 3.0039241262168814E-4 36-37 0.0 0.0 0.0 0.06745478510093686 3.0039241262168814E-4 38-39 0.0 0.0 0.0 0.10807451467433624 3.0039241262168814E-4 40-41 0.0 0.0 0.0 0.15471878096753727 3.0039241262168814E-4 42-43 0.0 0.0 0.0 0.2038162519638154 3.0039241262168814E-4 44-45 0.0 0.0 0.0 0.2627599187071378 3.170808799895597E-4 46-47 0.0 0.0 0.0 0.33271797391325536 3.3376934735743127E-4 48-49 0.0 0.0 0.0 0.4027427829888444 3.3376934735743127E-4 50-51 0.0 1.6688467367871563E-5 0.0 0.47745705139480543 3.3376934735743127E-4 52-53 0.0 3.3376934735743127E-5 0.0 0.5503188999229327 3.5045781472530283E-4 54-55 0.0 3.3376934735743127E-5 0.0 0.6330603211328398 3.671462820931744E-4 56-57 0.0 3.3376934735743127E-5 0.0 0.7150173843764571 3.671462820931744E-4 58-59 0.0 6.675386947148625E-5 0.0 0.7763308134860172 3.671462820931744E-4 60-61 0.0 6.675386947148625E-5 0.0 0.8270804427517147 3.671462820931744E-4 62-63 3.3376934735743127E-5 6.675386947148625E-5 0.0 0.8709544234618489 3.671462820931744E-4 64-65 3.3376934735743127E-5 6.675386947148625E-5 0.0 0.9240070612243128 3.671462820931744E-4 66-67 3.3376934735743127E-5 6.675386947148625E-5 0.0 0.9690659231175659 3.671462820931744E-4 68-69 3.3376934735743127E-5 6.675386947148625E-5 0.0 1.0232199997263092 3.671462820931744E-4 70-71 3.3376934735743127E-5 6.675386947148625E-5 0.0 1.1075301368687964 3.8383474946104596E-4 72-73 3.3376934735743127E-5 6.675386947148625E-5 0.0 1.222179907686074 4.505886189325322E-4 74-75 3.3376934735743127E-5 6.675386947148625E-5 0.0 1.3738613875926586 4.6727708630040377E-4 76-77 3.3376934735743127E-5 6.675386947148625E-5 0.0 1.5693834712746417 4.6727708630040377E-4 78-79 3.3376934735743127E-5 6.675386947148625E-5 0.0 1.8234653869504864 4.6727708630040377E-4 80-81 3.3376934735743127E-5 6.675386947148625E-5 0.0 2.12287317999747 5.006540210361469E-4 82-83 3.3376934735743127E-5 6.675386947148625E-5 0.0 2.4198444568087445 5.3403095577189E-4 84-85 3.3376934735743127E-5 8.344233683935782E-5 0.0 2.687043507835736 5.3403095577189E-4 86-87 3.3376934735743127E-5 1.0013080420722938E-4 0.0 2.93783779544011 5.674078905076332E-4 88-89 3.3376934735743127E-5 1.335077389429725E-4 0.0 3.185344454973013 6.007848252433763E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9145 0.0 29.350733 1 GTATCAA 13105 0.0 27.536882 1 TACATGG 10245 0.0 23.567732 2 GTACATG 10745 0.0 23.311945 1 ATCAACG 15555 0.0 22.794003 3 TCAACGC 15590 0.0 22.773071 4 ACATGGG 10090 0.0 22.523125 3 CAACGCA 15835 0.0 22.390951 5 TATCAAC 16040 0.0 22.226816 2 AACGCAG 16140 0.0 22.013752 6 ACGCAGA 18020 0.0 19.48174 7 CGCAGAG 17920 0.0 19.274904 8 GAGTACT 8905 0.0 19.156145 12-13 CATGGGA 6175 0.0 19.012321 4 AGAGTAC 14350 0.0 18.405909 10-11 CATGGGG 4600 0.0 17.937132 4 GCAGAGT 18900 0.0 17.47792 9 AGTACTT 9480 0.0 17.3729 12-13 GTACTTT 9705 0.0 17.358574 14-15 ATTATAC 1620 0.0 16.298431 3 >>END_MODULE