FastQCFastQC Report
Fri 27 May 2016
SRR939691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281885
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169640.743420461592061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112260.4919616895680544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83370.3653558351976546No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40570.1777916065007658No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36530.16008694566115297No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32230.1412428759556244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23960.10500090933592184No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.10473796882840283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT1404.620233E-1033.892016
GTACATG135100.027.5047681
GTATCAA221050.027.3514581
TACATGG139150.026.4666612
ACATGGG137750.025.633153
GGTATCA156650.023.2362771
CATGGGA95600.023.2331774
ATCAACG262100.023.1592313
TCAACGC262300.023.1415734
CAACGCA263500.023.1262425
TATCAAC263750.022.9783612
AACGCAG266450.022.9364136
GAGTACT117750.022.64896812-13
CATGGGG39750.021.3715744
AGTACTT122250.021.36886612-13
GTATAGG8300.021.1555941
ATGGGAT40650.021.1319055
ACGCAGA290350.020.6734587
CATTCCG3001.6916601E-1020.563419
CGCAGAG290150.020.459658
Produced by FastQC (version 0.11.5)