FastQCFastQC Report
Fri 27 May 2016
SRR939691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281885
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169120.7411416438602295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127070.5568641715073284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94050.4121592455360371No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39950.17507455458973611No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39790.1743733799030188No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34270.1501828532112705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28100.12314380435473303No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.1089888403666267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24210.1060964947839177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.10184562324569381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211450.026.2256621
GTACATG132950.026.1310541
TACATGG134900.025.3622322
GGTATCA148950.025.3151721
ACATGGG132650.024.5845573
CATGGGA94700.022.8578174
ATCAACG247300.022.149573
TCAACGC247350.022.1446064
AACGCAG251400.021.8718576
CAACGCA251300.021.8152565
GAGTACT120450.021.59660112-13
TATCAAC258350.021.2946782
CATGGGG37400.020.6347474
AGTACTT125300.020.62938112-13
ATGGGAT42100.020.3430565
ACGCAGA277300.019.591747
GTACTTT134050.019.5630814-15
CGCAGAG274850.019.5616238
GCAGAGT282000.018.615679
ATGGGAG38000.018.3275745
Produced by FastQC (version 0.11.5)