##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939691_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2281885 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56311777324449 31.0 31.0 34.0 30.0 34.0 2 31.759170597992448 31.0 31.0 34.0 30.0 34.0 3 31.792784912473678 31.0 31.0 34.0 30.0 34.0 4 35.58676445131985 37.0 35.0 37.0 33.0 37.0 5 35.33459836933062 37.0 35.0 37.0 33.0 37.0 6 35.368803861719584 37.0 35.0 37.0 33.0 37.0 7 35.30234564844416 37.0 35.0 37.0 32.0 37.0 8 35.269969345519165 37.0 35.0 37.0 32.0 37.0 9 36.85474202249456 39.0 37.0 39.0 32.0 39.0 10-11 36.748290119791314 39.0 36.0 39.0 32.0 39.0 12-13 36.69254497926057 39.0 35.0 39.0 32.0 39.0 14-15 37.69255834540303 39.5 36.5 41.0 32.0 41.0 16-17 37.67161995455511 39.0 36.5 41.0 32.0 41.0 18-19 37.72368677650276 39.5 37.0 41.0 32.0 41.0 20-21 37.68322921619625 39.0 37.0 41.0 32.0 41.0 22-23 37.54372919757131 39.0 36.0 41.0 32.0 41.0 24-25 37.419245053979495 39.0 36.0 41.0 32.0 41.0 26-27 37.155435089848964 39.0 36.0 40.5 31.0 41.0 28-29 36.94759354656348 39.0 36.0 40.0 31.0 41.0 30-31 36.670061812930975 38.5 35.0 40.0 30.0 41.0 32-33 36.444513198517896 38.0 35.0 40.0 30.0 41.0 34-35 36.37915648685188 38.0 35.0 40.0 30.0 41.0 36-37 36.21546791358898 38.0 35.0 40.0 30.0 41.0 38-39 36.20056597944243 38.0 35.0 40.0 30.0 41.0 40-41 36.07289806453875 38.0 35.0 40.0 30.0 41.0 42-43 35.8465838550146 38.0 35.0 40.0 29.0 41.0 44-45 35.5608444334399 38.0 35.0 40.0 27.5 41.0 46-47 35.14862493070422 38.0 34.0 40.0 26.5 41.0 48-49 34.77619928261065 38.0 34.0 40.0 24.5 41.0 50-51 34.51527509055013 38.0 34.0 40.0 24.0 41.0 52-53 34.27011067604195 38.0 33.0 40.0 23.5 41.0 54-55 33.99816270320371 38.0 33.0 40.0 23.0 41.0 56-57 32.053755776474276 37.0 30.0 39.5 14.0 41.0 58-59 27.11622540136773 32.5 17.0 38.5 4.0 40.0 60-61 27.840942028191606 33.0 19.0 38.0 4.0 40.0 62-63 30.925095918505974 34.5 29.0 38.5 2.0 40.0 64-65 31.171904587654502 35.5 30.5 39.0 2.0 40.0 66-67 24.57679352815764 27.0 17.5 35.5 2.0 39.0 68-69 17.205632623905238 13.0 7.0 28.0 2.0 35.5 70-71 16.1069858910506 12.5 7.0 26.5 2.0 33.5 72-73 17.22049971843454 13.5 7.0 28.5 2.0 34.0 74-75 18.2043356698519 17.0 7.5 29.5 2.0 34.5 76-77 19.35736529229124 21.0 8.0 30.0 2.0 34.5 78-79 22.37854843692824 27.5 10.0 33.5 2.0 36.0 80-81 26.00042464891964 31.5 22.5 34.5 2.0 36.0 82-83 27.293180418820405 33.5 26.0 35.0 2.0 36.0 84-85 27.332725137331636 34.0 26.0 35.0 2.0 36.0 86-87 27.091201134150054 34.0 26.0 35.0 2.0 36.0 88-89 26.659423897348027 33.0 25.5 35.0 2.0 35.5 90-91 25.30562648862673 32.5 14.5 35.0 2.0 35.0 92-93 22.90072177169314 31.5 2.0 34.5 2.0 35.0 94-95 15.681268994712706 4.5 2.0 32.0 2.0 35.0 96-97 13.15191585027291 2.0 2.0 30.0 2.0 34.0 98-99 12.851066333316535 2.0 2.0 29.5 2.0 34.0 100-101 12.570783584624115 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3581696710967712 1101 2 0.3389257112974775 1101 3 0.45602882559970226 1101 4 0.32181575914975014 1101 5 0.43039315731128625 1101 6 0.4371821539810412 1101 7 0.44566041865182626 1101 8 0.3608446746739773 1101 9 0.42813473524258683 1101 10-11 0.5240141818053345 1101 12-13 0.6508778953238874 1101 14-15 0.7021514259440593 1101 16-17 0.7061291116023156 1101 18-19 0.44765986620235054 1101 20-21 0.5242326738312002 1101 22-23 0.5157626265972368 1101 24-25 0.5445535380797963 1101 26-27 0.6680237388089978 1101 28-29 0.5973007095393257 1101 30-31 0.5897965197660682 1101 32-33 0.5427085172940949 1101 34-35 0.5294615501668787 1101 36-37 0.44793036141362563 1101 38-39 0.48293508401406626 1101 40-41 0.6030202725006575 1101 42-43 0.6493559782857048 1101 44-45 0.5457749797111049 1101 46-47 0.37380260063874715 1101 48-49 0.3935718161694268 1101 50-51 0.32770692803043744 1101 52-53 0.35824776198771957 1101 54-55 0.46329307559246047 1101 56-57 -1.3346680781071782 1101 58-59 -9.36664627209107 1101 60-61 -9.557031830239051 1101 62-63 -3.5988803442121338 1101 64-65 -4.559640847641511 1101 66-67 -9.585307129893957 1101 68-69 -4.743662356422856 1101 70-71 -4.499595269120958 1101 72-73 -4.61550288533677 1101 74-75 -6.4231506905966285 1101 76-77 -7.645958249950979 1101 78-79 -11.307766592215298 1101 80-81 -8.16909570749684 1101 82-83 -4.2761815813458135 1101 84-85 -3.4333314106488544 1101 86-87 -3.2017131520579873 1101 88-89 -3.212665647840435 1101 90-91 -9.239816203502693 1101 92-93 -16.810158009935478 1101 94-95 -10.908587705937174 1101 96-97 -9.087014217918384 1101 98-99 -9.229858658624998 1101 100-101 -9.098009485257503 1102 1 0.3384156756615049 1102 2 0.30160099005397 1102 3 0.37604983153995875 1102 4 0.2363323560606787 1102 5 0.2575340304866316 1102 6 0.2147821936192429 1102 7 0.27508250467535333 1102 8 0.3696010867141979 1102 9 0.5142662920011176 1102 10-11 0.37979350881442286 1102 12-13 0.3242310592788229 1102 14-15 0.38456275348088553 1102 16-17 0.32041588762547235 1102 18-19 0.42178701205911295 1102 20-21 0.493564202704718 1102 22-23 0.5524115396147238 1102 24-25 0.39250316876183433 1102 26-27 0.40302279826600085 1102 28-29 0.4746242324187193 1102 30-31 0.4775623743970101 1102 32-33 0.5133058182669856 1102 34-35 0.42441226024083534 1102 36-37 0.5082439410395878 1102 38-39 0.32986840974239584 1102 40-41 0.19828964372533875 1102 42-43 0.41497669777084667 1102 44-45 0.3156835958050763 1102 46-47 0.2232830262538883 1102 48-49 0.10418704651316091 1102 50-51 0.04068028545779612 1102 52-53 0.010870234944583501 1102 54-55 -0.025849198170945442 1102 56-57 -2.6700324369722885 1102 58-59 -10.365128728299648 1102 60-61 -10.535661627620453 1102 62-63 -4.696598872102474 1102 64-65 -5.550433752749797 1102 66-67 -7.044068280657669 1102 68-69 -4.17025405619577 1102 70-71 -4.33745660398912 1102 72-73 -4.532490100001521 1102 74-75 -5.332293229382865 1102 76-77 -7.564476835685435 1102 78-79 -10.173571416149397 1102 80-81 -6.134226192353232 1102 82-83 -3.6567343498405904 1102 84-85 -3.159841241411609 1102 86-87 -2.9999125235505737 1102 88-89 -3.225465386504876 1102 90-91 -9.102593429629682 1102 92-93 -13.410097400365677 1102 94-95 -10.528789388313646 1102 96-97 -9.009442322552857 1102 98-99 -9.191630699234562 1102 100-101 -9.202949309230489 1103 1 0.18466599798322036 1103 2 0.11330544380201601 1103 3 0.23421630252542514 1103 4 0.14889517137596187 1103 5 0.18097144887394734 1103 6 0.21254116737580375 1103 7 0.13097285273739345 1103 8 0.16219973367524432 1103 9 0.3878364893869062 1103 10-11 0.37718727064793 1103 12-13 0.301046811824925 1103 14-15 0.5086454140074252 1103 16-17 0.3627389740357714 1103 18-19 0.36426097290318893 1103 20-21 0.1824828182521756 1103 22-23 0.3608763633478489 1103 24-25 0.38639737869737445 1103 26-27 0.3869520284946546 1103 28-29 0.34648016343764 1103 30-31 0.3250227063744404 1103 32-33 0.4161060791385438 1103 34-35 0.3223683534683488 1103 36-37 0.17729288088243322 1103 38-39 0.2839683522870686 1103 40-41 0.2953352671383769 1103 42-43 0.1605337161468512 1103 44-45 0.10531234105955178 1103 46-47 0.033662373880936514 1103 48-49 -0.0030898784726431927 1103 50-51 0.08228647056635907 1103 52-53 0.07662159396029011 1103 54-55 0.004110669807737111 1103 56-57 -2.6929016401104313 1103 58-59 -10.220799722111689 1103 60-61 -10.555313497458013 1103 62-63 -4.451061053674941 1103 64-65 -5.6120248132537895 1103 66-67 -6.652211783962848 1103 68-69 -4.262060467863723 1103 70-71 -4.124827497238849 1103 72-73 -4.480885386889007 1103 74-75 -5.395274944219036 1103 76-77 -6.9632888750027995 1103 78-79 -9.502875616210382 1103 80-81 -5.433298318714492 1103 82-83 -3.4207462348151445 1103 84-85 -3.0003435533537903 1103 86-87 -2.8076554911565452 1103 88-89 -2.8958027874057883 1103 90-91 -5.60894365774778 1103 92-93 -10.172713263280523 1103 94-95 -8.882498890615885 1103 96-97 -8.532003397485713 1103 98-99 -8.509058905362732 1103 100-101 -8.388342772948675 1104 1 0.2517011275494738 1104 2 0.2516182813628447 1104 3 0.12036388460859371 1104 4 0.13245681625111416 1104 5 0.18613660740766136 1104 6 0.14564225259788088 1104 7 0.15765843011450187 1104 8 0.1710831006164497 1104 9 0.22376805340776684 1104 10-11 0.2360340322361978 1104 12-13 0.2456542523775127 1104 14-15 0.37486788386990355 1104 16-17 0.24859240426751938 1104 18-19 0.25376516814792893 1104 20-21 0.2650704325336335 1104 22-23 0.37223255989705706 1104 24-25 0.3430247308924663 1104 26-27 0.39413290029585113 1104 28-29 0.4507096136440225 1104 30-31 0.434835540046123 1104 32-33 0.43448729728196867 1104 34-35 0.48096951106639096 1104 36-37 0.4713584123373309 1104 38-39 0.40444464114360557 1104 40-41 0.3156420843091112 1104 42-43 0.3170423262304993 1104 44-45 0.24681379986680696 1104 46-47 0.2794860531334322 1104 48-49 0.26894357496874477 1104 50-51 0.3115635627486739 1104 52-53 0.3012945124683739 1104 54-55 0.22164568677862206 1104 56-57 -2.865206897071662 1104 58-59 -9.830943132759248 1104 60-61 -10.434654988122084 1104 62-63 -4.229173613759798 1104 64-65 -5.425563083443603 1104 66-67 -6.6886495244137265 1104 68-69 -4.156564580582788 1104 70-71 -4.178252720537193 1104 72-73 -4.577762368809434 1104 74-75 -4.869173496832568 1104 76-77 -6.743934214379667 1104 78-79 -9.19930673184175 1104 80-81 -4.912811842205944 1104 82-83 -2.7336118733447314 1104 84-85 -2.1339036710297066 1104 86-87 -1.9980417435557207 1104 88-89 -2.027797193872612 1104 90-91 -4.48246699363656 1104 92-93 -6.681156019015724 1104 94-95 -7.304170834182743 1104 96-97 -7.512533409606553 1104 98-99 -7.819762940412808 1104 100-101 -7.951915523692499 1105 1 0.3205756800877424 1105 2 0.3530976119732756 1105 3 0.3801779137696357 1105 4 0.1927337011454071 1105 5 0.3028589184474626 1105 6 0.2570697802741293 1105 7 0.24475703269693128 1105 8 0.29820893222207445 1105 9 0.37332092121142324 1105 10-11 0.37179820554535326 1105 12-13 0.34267638396186584 1105 14-15 0.5196582147420727 1105 16-17 0.47177162810854867 1105 18-19 0.4255854829576222 1105 20-21 0.38454615143822224 1105 22-23 0.5890061842758882 1105 24-25 0.4702887789782437 1105 26-27 0.6776212093283291 1105 28-29 0.620018587735018 1105 30-31 0.5928711147837689 1105 32-33 0.4678873850324905 1105 34-35 0.6194922706506887 1105 36-37 0.5737229686605474 1105 38-39 0.3929823710414908 1105 40-41 0.5150956207867736 1105 42-43 0.5687408448285254 1105 44-45 0.5351861972448404 1105 46-47 0.6178099617599102 1105 48-49 0.48168679548805926 1105 50-51 0.5448295767473326 1105 52-53 0.6580225856666075 1105 54-55 0.5826267413289372 1105 56-57 1.738199649568866 1105 58-59 -8.495782479639306 1105 60-61 -10.02175340239868 1105 62-63 -3.6899592068572566 1105 64-65 -4.97178482109215 1105 66-67 -6.343069815496882 1105 68-69 -4.098333826851039 1105 70-71 -3.9508687216368994 1105 72-73 -2.338415112142938 1105 74-75 -4.376402746000728 1105 76-77 -6.242450458582201 1105 78-79 -8.756477323743468 1105 80-81 -4.479684892063684 1105 82-83 -2.1343982384961 1105 84-85 -1.7151749613577536 1105 86-87 -1.5034319979757278 1105 88-89 -1.5630480454857363 1105 90-91 -4.085452205979632 1105 92-93 -6.301727301246622 1105 94-95 -7.465317127099995 1105 96-97 -7.812518250987136 1105 98-99 -8.162532278950914 1105 100-101 -8.20428855377812 1106 1 0.1565242465571579 1106 2 0.1539782557189362 1106 3 0.07665691312218925 1106 4 0.06509821484310407 1106 5 -0.0037064528693306897 1106 6 0.17822766444635363 1106 7 0.07401683932921088 1106 8 0.09232465766589115 1106 9 0.14063969063231951 1106 10-11 0.1883346287738803 1106 12-13 0.1888496671001363 1106 14-15 0.25786093401253396 1106 16-17 0.2212741381892087 1106 18-19 0.22049221343733905 1106 20-21 0.22256044246588402 1106 22-23 0.3230205815711784 1106 24-25 0.19875295596500564 1106 26-27 0.182695980410152 1106 28-29 0.20731409635352804 1106 30-31 0.3053759607842679 1106 32-33 0.1688755755506648 1106 34-35 0.3121797788913483 1106 36-37 0.24678510963832423 1106 38-39 0.34099792153731556 1106 40-41 0.24299146277074612 1106 42-43 0.1689398998338234 1106 44-45 0.2744478537209645 1106 46-47 0.22518503334296014 1106 48-49 0.34591514493536124 1106 50-51 0.46180081167553055 1106 52-53 0.559429254156889 1106 54-55 0.33607532255774686 1106 56-57 1.5250294501596393 1106 58-59 -8.401621295266938 1106 60-61 -9.749864337927686 1106 62-63 -3.527535734306774 1106 64-65 -4.932653539248761 1106 66-67 -6.410701188848503 1106 68-69 -3.594190373428729 1106 70-71 -3.7612361124321563 1106 72-73 -4.286305798179175 1106 74-75 -5.065813767527793 1106 76-77 -6.632214426379177 1106 78-79 -8.908069075014254 1106 80-81 -4.52920363747597 1106 82-83 -2.3465950067795447 1106 84-85 -1.8786259828061063 1106 86-87 -1.75895250270759 1106 88-89 -1.6601097456496632 1106 90-91 -4.195666689469775 1106 92-93 -6.29016479325195 1106 94-95 -7.253285860803166 1106 96-97 -6.7708903687237925 1106 98-99 -6.187172592558484 1106 100-101 -7.071523981830019 1107 1 0.05337633090172744 1107 2 -0.03369815773582019 1107 3 4.999544829118463E-4 1107 4 0.10350392453543833 1107 5 0.005649189900481133 1107 6 -0.024965509553162235 1107 7 0.14291586497206765 1107 8 0.08190211457155527 1107 9 0.1465234683627088 1107 10-11 0.0750298336089017 1107 12-13 0.012133010389028698 1107 14-15 0.04899300056256095 1107 16-17 0.10771834738891073 1107 18-19 0.09928689398966384 1107 20-21 0.17054055967438586 1107 22-23 0.2134112411753577 1107 24-25 0.2786231253122793 1107 26-27 0.27831643949766516 1107 28-29 0.1520094613779861 1107 30-31 0.2843530257082776 1107 32-33 0.31806879008004074 1107 34-35 0.17565349672438657 1107 36-37 0.18078560950660716 1107 38-39 0.01117103229094596 1107 40-41 0.1027058430547001 1107 42-43 0.13023388317605367 1107 44-45 0.08292892000368823 1107 46-47 0.1323593011276074 1107 48-49 0.09467034747369496 1107 50-51 0.028120208205208996 1107 52-53 0.0675351297321427 1107 54-55 -0.07928240653487961 1107 56-57 1.124185174568165 1107 58-59 -9.117626826627887 1107 60-61 -10.46533082214565 1107 62-63 -3.87875370486087 1107 64-65 -5.035131650711669 1107 66-67 -6.96284343321847 1107 68-69 -4.112232819772501 1107 70-71 -4.083168170876215 1107 72-73 -2.454070180730877 1107 74-75 -4.692995697386044 1107 76-77 -6.485983462144848 1107 78-79 -7.277491321854612 1107 80-81 -2.1433113098750383 1107 82-83 -1.0535289903919711 1107 84-85 -0.6983977996921951 1107 86-87 -0.6758088716373969 1107 88-89 -0.5360214986336764 1107 90-91 -0.1643708822838299 1107 92-93 -3.09955324590873 1107 94-95 -5.894100684696097 1107 96-97 -6.600941523197566 1107 98-99 -6.4296194188291675 1107 100-101 -7.23999869843462 1108 1 -0.18942675001012432 1108 2 -0.13906826797988714 1108 3 -0.168547389440306 1108 4 -0.15145615577337423 1108 5 -0.1089524617744857 1108 6 -0.17633191490322986 1108 7 -0.1988304545082329 1108 8 -0.16912539769684543 1108 9 -0.20267597120888325 1108 10-11 -0.17683155063482303 1108 12-13 -0.21960242190277057 1108 14-15 -0.2485286745117392 1108 16-17 -0.20945641816640403 1108 18-19 -0.19249481336704122 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2316 84-85 1.9764348814586405 2316 86-87 1.8636200251456607 2316 88-89 1.8700019799362515 2316 90-91 2.724495363425998 2316 92-93 4.715981546686624 2316 94-95 11.646732894716036 2316 96-97 13.506443846813594 2316 98-99 13.202041551186943 2316 100-101 12.161473619026482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 53.0 5 93.0 6 294.0 7 627.0 8 1382.0 9 2442.0 10 4208.0 11 6922.0 12 10119.0 13 13941.0 14 17227.0 15 22846.0 16 31053.0 17 41489.0 18 42319.0 19 38223.0 20 38409.0 21 37784.0 22 38309.0 23 32713.0 24 33137.0 25 39442.0 26 55381.0 27 82241.0 28 115834.0 29 156154.0 30 195028.0 31 214930.0 32 216479.0 33 199355.0 34 178064.0 35 159980.0 36 131206.0 37 86412.0 38 35119.0 39 2645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.715561263626675 20.689322643849966 14.672324085965888 21.92279200655746 2 15.052541020212198 22.47906313643836 40.10871523104507 22.359680612304373 3 18.24820685058235 27.927028558481847 31.65119622907262 22.173568361863182 4 11.884779469605173 18.31516487465407 39.16998446459834 30.63007119114241 5 11.965926377169321 39.62399461394843 34.72862147451062 13.68145753437163 6 28.42496444825221 38.761068151988376 18.598833858849154 14.215133540910257 7 25.644324757820836 35.33644333522504 22.237711365822555 16.781520541131563 8 25.369710818103375 34.97853277887119 20.898472336712608 18.75328406631283 9 26.806171213711472 16.47409926442393 21.486446512422845 35.233283009441756 10-11 23.75038619387042 27.582064827982126 28.524180666422716 20.143368311724736 12-13 25.58766107845049 25.733001443981617 28.60209870348418 20.077238774083707 14-15 22.496471094528214 26.32589776187853 28.10319084925559 23.074440294337666 16-17 20.411633364521 29.678489494431137 28.62885290012424 21.28102424092362 18-19 20.24418905555705 27.983532872791923 31.84223794464169 19.930040127009335 20-21 21.586320081862144 26.93003810446188 31.48169167157854 20.00195014209743 22-23 21.029061401465594 27.23858072011378 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21.19229981173359 48-49 20.995967481872096 27.93722065565384 29.839405656581786 21.227406205892276 50-51 20.903084758634897 28.2055601952305 29.71326808831572 21.178086957818884 52-53 21.04579575578492 28.48680281544031 29.461192730631897 21.006208698142874 54-55 20.748434921963195 28.11248123967689 29.62021474703936 21.51886909132056 56-57 20.29009359137056 28.578856457980823 29.86322617033277 21.267823780315847 58-59 20.10020322412142 27.820115827288657 30.728640045367655 21.351040903222273 60-61 21.039561112371356 28.16505416720808 29.726311894762112 21.069072825658452 62-63 21.267012845518728 28.046009049559583 29.505271897653067 21.181706207268615 64-65 20.5667242964483 28.136539804365867 29.896584486641935 21.4001514125439 66-67 20.888929479208095 28.063419743821 29.55863461410925 21.48901616286166 68-69 21.3253240252185 27.69583570094975 29.642828151635687 21.336012122196067 70-71 21.165577207070918 27.912777147594646 29.436834060077793 21.484811585256644 72-73 20.432532793231573 27.83921205367486 30.0006613307324 21.727593822361165 74-75 20.782485773412677 28.13388393322166 29.434986876534815 21.648643416830847 76-77 18.945358805937783 28.506962274376292 30.489133465867667 22.058545453818258 78-79 19.617211653714655 28.45537858649836 29.898094130239596 22.029315629547394 80-81 20.3268667223071 28.694460137619092 28.995374477877668 21.98329866219614 82-83 20.734412619780723 28.723380478710958 28.49576094778073 22.046445953727588 84-85 20.57792259014497 28.400091152909006 28.39936806492892 22.622618192017107 86-87 20.654917413329517 28.417304099193842 28.66188361284391 22.26589487463273 88-89 20.916379750657516 28.051325189322935 28.580515684096653 22.451779375922897 90-91 20.020278125740635 28.314475247485603 29.158020677450185 22.507225949323576 92-93 19.161471569905377 28.614663348491533 29.87576199762707 22.34810308397602 94-95 18.794154239142244 28.542922960897275 29.59954007840304 23.06338272155744 96-97 18.947446873086253 28.731275602655764 28.87490189951277 23.446375624745208 98-99 18.892694748846576 28.476328632525927 28.98854197638352 23.642434642243977 100-101 19.321117469961454 28.41783905589318 28.969125357422705 23.29191811672266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1579.0 1 1170.5 2 785.0 3 856.5 4 985.5 5 1205.5 6 1552.0 7 2185.5 8 2933.5 9 3406.5 10 3604.5 11 3832.5 12 4237.5 13 4570.0 14 4892.0 15 5258.0 16 5648.0 17 6048.5 18 6323.0 19 6740.5 20 7402.0 21 8164.5 22 9216.5 23 10628.0 24 12384.0 25 14866.0 26 18252.0 27 21941.0 28 25579.5 29 29962.0 30 35153.0 31 40644.5 32 47003.0 33 54182.5 34 61042.5 35 67249.0 36 72979.0 37 77024.0 38 79008.5 39 79708.0 40 80100.0 41 80623.0 42 80975.5 43 82227.0 44 83571.0 45 83833.0 46 84631.5 47 85957.0 48 86711.5 49 83672.5 50 76686.5 51 70183.5 52 64603.5 53 59108.5 54 53927.0 55 48498.5 56 43187.0 57 38232.0 58 33254.0 59 28398.0 60 23615.0 61 19107.0 62 14754.0 63 10847.0 64 7866.5 65 5686.5 66 3963.5 67 2607.0 68 1703.5 69 1163.0 70 809.0 71 602.5 72 467.0 73 347.5 74 233.0 75 148.0 76 85.0 77 41.5 78 19.0 79 11.5 80 5.5 81 2.5 82 2.0 83 1.0 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02256906022871442 2 0.00631057218045607 3 2.629405075190029E-4 4 0.0 5 0.14930638485287384 6 0.0 7 0.0 8 0.00148999620927435 9 0.0 10-11 0.0 12-13 0.0 14-15 7.011746867173411E-4 16-17 0.0 18-19 2.41028798559086E-4 20-21 0.0 22-23 4.163224702384213E-4 24-25 0.0 26-27 2.8485221647891985E-4 28-29 0.003703078814225958 30-31 0.012402027271312972 32-33 0.0029142572916689494 34-35 0.004623370590542468 36-37 0.113130153360051 38-39 0.11293294797941175 40-41 0.12597041481056231 42-43 0.1281396739975941 44-45 0.12417365467584913 46-47 0.11692087901011662 48-49 0.11674558533843729 50-51 0.11527750083812287 52-53 0.09200726592269111 54-55 0.07918891618113971 56-57 0.5453824360123319 58-59 1.5491797351750856 60-61 0.5083516478700723 62-63 0.00510542818766064 64-65 0.1175124951520344 66-67 1.8716324442292227 68-69 2.825076636202087 70-71 2.739511412713612 72-73 2.58970105855466 74-75 1.9743326241243535 76-77 1.3870330888717004 78-79 0.9191961908685145 80-81 0.051251487257245654 82-83 3.0676392543883677E-4 84-85 2.1911708959916913E-4 86-87 4.163224702384213E-4 88-89 1.5338196271941839E-4 90-91 0.2428036469848393 92-93 0.38336725996270626 94-95 1.971264984869965 96-97 2.391750679810771 98-99 1.3956005670750278 100-101 0.9238633848769767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2281885.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.296684185768434 #Duplication Level Percentage of deduplicated Percentage of total 1 70.25138070989294 30.416518442104195 2 14.780199405744614 12.798672517464137 3 5.739857851704574 7.455504380293686 4 2.923646997821504 5.063368829413906 5 1.6133537092839243 3.4926433015402067 6 1.0411427678657807 2.7046817767548914 7 0.7048770324275582 2.136318678397235 8 0.5043900894011727 1.7470734725787265 9 0.35450920804172575 1.3814165899376531 >10 1.8243941612152754 14.267830494051728 >50 0.1517485353975058 4.569905311807992 >100 0.10127782645607507 8.346680747531805 >500 0.006384257132683103 1.8423862172327468 >1k 0.0025334353701123237 2.0647923455733976 >5k 1.0133741480449296E-4 0.41265133893153016 >10k+ 2.026748296089859E-4 1.2995555563862828 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16912 0.7411416438602295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12707 0.5568641715073284 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9405 0.4121592455360371 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3995 0.17507455458973611 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3979 0.1743733799030188 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3427 0.1501828532112705 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2810 0.12314380435473303 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2487 0.1089888403666267 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2421 0.1060964947839177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2324 0.10184562324569381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.382341791983382E-5 0.0 9 0.0 0.0 0.0 4.382341791983382E-5 0.0 10-11 0.0 0.0 0.0 4.382341791983382E-5 0.0 12-13 0.0 0.0 0.0 1.0955854479958455E-4 0.0 14-15 0.0 0.0 0.0 2.191170895991691E-4 0.0 16-17 0.0 0.0 0.0 3.067639254388367E-4 0.0 18-19 0.0 0.0 0.0 3.944107612785044E-4 0.0 20-21 0.0 0.0 0.0 6.573512687975073E-4 0.0 22-23 0.0 0.0 0.0 0.0013585259555148484 0.0 24-25 0.0 0.0 0.0 0.0023445528587111098 0.0 26-27 0.0 0.0 0.0 0.0033524914708672874 0.0 28-29 0.0 0.0 0.0 0.0067926297775742425 0.0 30-31 0.0 0.0 0.0 0.01759510229481328 0.0 32-33 0.0 0.0 0.0 0.03952872296369011 0.0 34-35 0.0 0.0 0.0 0.07222099273188615 0.0 36-37 0.0 0.0 0.0 0.11963793092114633 0.0 38-39 0.0 0.0 0.0 0.18872554927176435 0.0 40-41 0.0 0.0 0.0 0.26833078792314247 0.0 42-43 0.0 0.0 0.0 0.3457886790964487 0.0 44-45 0.0 0.0 0.0 0.4408416725645683 0.0 46-47 0.0 0.0 0.0 0.5503125705283132 0.0 48-49 0.0 0.0 0.0 0.6539549539087202 0.0 50-51 0.0 0.0 0.0 0.7526014676462661 0.0 52-53 0.0 0.0 0.0 0.8485090177638225 0.0 54-55 0.0 0.0 0.0 0.9518008138008708 0.0 56-57 0.0 0.0 0.0 1.0357664825352724 0.0 58-59 0.0 0.0 0.0 1.0955197128689658 0.0 60-61 0.0 0.0 0.0 1.1455222327154961 0.0 62-63 4.382341791983382E-5 0.0 0.0 1.1890169750009312 0.0 64-65 8.764683583966764E-5 2.191170895991691E-5 0.0 1.2301452527186953 0.0 66-67 8.764683583966764E-5 4.382341791983382E-5 0.0 1.2649410465470434 0.0 68-69 8.764683583966764E-5 4.382341791983382E-5 0.0 1.3132782765126199 0.0 70-71 8.764683583966764E-5 4.382341791983382E-5 0.0 1.3915030774995234 0.0 72-73 8.764683583966764E-5 4.382341791983382E-5 0.0 1.502091472620224 0.0 74-75 8.764683583966764E-5 4.382341791983382E-5 0.0 1.6465553697929562 0.0 76-77 8.764683583966764E-5 4.382341791983382E-5 0.0 1.876080521148086 0.0 78-79 8.764683583966764E-5 4.382341791983382E-5 0.0 2.2241041945584463 0.0 80-81 8.764683583966764E-5 4.382341791983382E-5 0.0 2.642113866386781 0.0 82-83 8.764683583966764E-5 4.382341791983382E-5 0.0 3.058282954662483 0.0 84-85 8.764683583966764E-5 4.382341791983382E-5 0.0 3.3796619899775844 0.0 86-87 8.764683583966764E-5 4.382341791983382E-5 0.0 3.6290829730683187 0.0 88-89 8.764683583966764E-5 4.382341791983382E-5 0.0 3.8629685545064714 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21145 0.0 26.225662 1 GTACATG 13295 0.0 26.131054 1 TACATGG 13490 0.0 25.362232 2 GGTATCA 14895 0.0 25.315172 1 ACATGGG 13265 0.0 24.584557 3 CATGGGA 9470 0.0 22.857817 4 ATCAACG 24730 0.0 22.14957 3 TCAACGC 24735 0.0 22.144606 4 AACGCAG 25140 0.0 21.871857 6 CAACGCA 25130 0.0 21.815256 5 GAGTACT 12045 0.0 21.596601 12-13 TATCAAC 25835 0.0 21.294678 2 CATGGGG 3740 0.0 20.634747 4 AGTACTT 12530 0.0 20.629381 12-13 ATGGGAT 4210 0.0 20.343056 5 ACGCAGA 27730 0.0 19.59174 7 GTACTTT 13405 0.0 19.56308 14-15 CGCAGAG 27485 0.0 19.561623 8 GCAGAGT 28200 0.0 18.61567 9 ATGGGAG 3800 0.0 18.327574 5 >>END_MODULE