FastQCFastQC Report
Fri 27 May 2016
SRR939690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225785
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68480.307666733309821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.21138609524280197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.1566638287166101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG107600.031.1399691
GTATCAA194250.030.8206751
GGTATCA143450.026.0231321
TACATGG130150.025.8835622
ACATGGG128350.024.8109113
GTGTAGG9350.023.6753981
GAGTACT109050.023.45862812-13
CATGGGA89000.023.3025954
TATCAAC249750.023.2879492
ATCAACG264000.022.1025163
TCAACGC266650.021.9689794
AGTACTT117300.021.90931712-13
CAACGCA269150.021.67475
AACGCAG272850.021.4667856
ACGCAGA281800.020.550527
GTACTTT124750.020.5284714-15
ATGGGAT38650.020.1494625
CATATAG5551.6267222E-819.942791
CGCAGAG293000.019.7005378
CATGGGG39750.019.6099554
Produced by FastQC (version 0.11.5)