FastQCFastQC Report
Fri 27 May 2016
SRR939690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225785
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165630.7441419544115896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123320.5540517165853844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92890.41733590620837147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39060.175488647825374No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38030.1708610669943413No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31280.14053468776184583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26760.12022724566838217No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.11461124951421633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.11425182576034973No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.10243576985198481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131050.027.014551
GGTATCA150350.026.529361
CATTCCG4650.026.3648519
TACATGG133150.026.298962
ACATGGG131050.025.4925613
GTATCAA209450.025.4440981
CATGGGA94850.023.431544
GAGTACT118450.022.49301112-13
CATGGGG36950.021.8372974
GTCGGGA6800.021.514372
ATCAACG247950.021.2762413
TCAACGC248800.021.2035544
AGTACTT124300.021.1309112-13
ATGGGAT39750.021.1300325
CAACGCA252300.020.8720055
AACGCAG252250.020.8611376
TATCAAC256300.020.6595122
GTACTTT132250.020.23505414-15
CGGGAGT7300.020.0380784
CGCAGAG273100.019.0094938
Produced by FastQC (version 0.11.5)