##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939690_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2225785 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50040906915987 31.0 31.0 34.0 30.0 34.0 2 31.68947180432971 31.0 31.0 34.0 30.0 34.0 3 31.729179143538122 31.0 31.0 34.0 30.0 34.0 4 35.53960467879872 37.0 35.0 37.0 33.0 37.0 5 35.30977340578717 37.0 35.0 37.0 33.0 37.0 6 35.33028347302188 37.0 35.0 37.0 32.0 37.0 7 35.23409628513087 37.0 35.0 37.0 32.0 37.0 8 35.20234748639244 37.0 35.0 37.0 32.0 37.0 9 36.778528923503394 39.0 37.0 39.0 32.0 39.0 10-11 36.66739914232507 39.0 35.0 39.0 32.0 39.0 12-13 36.60453682633318 39.0 35.0 39.0 32.0 39.0 14-15 37.61813090662396 39.0 36.0 41.0 32.0 41.0 16-17 37.58588700166458 39.0 36.0 41.0 32.0 41.0 18-19 37.62382148320705 39.0 36.5 41.0 32.0 41.0 20-21 37.59127364053581 39.0 36.0 41.0 32.0 41.0 22-23 37.45271263846239 39.0 36.0 41.0 32.0 41.0 24-25 37.317744526088546 39.0 36.0 41.0 31.5 41.0 26-27 37.030103536505095 39.0 36.0 40.0 31.0 41.0 28-29 36.82694734666646 39.0 36.0 40.0 30.5 41.0 30-31 36.543621239248175 38.0 35.0 40.0 30.0 41.0 32-33 36.31721527461098 38.0 35.0 40.0 30.0 41.0 34-35 36.25028113676748 38.0 35.0 40.0 30.0 41.0 36-37 36.10236837789813 38.0 35.0 40.0 30.0 41.0 38-39 36.09781762389449 38.0 35.0 40.0 30.0 41.0 40-41 35.96198577131214 38.0 35.0 40.0 29.5 41.0 42-43 35.73449322373904 38.0 35.0 40.0 28.5 41.0 44-45 35.44878368755293 38.0 34.5 40.0 27.0 41.0 46-47 35.04228800176118 38.0 34.0 40.0 25.5 41.0 48-49 34.67460446539086 38.0 34.0 40.0 24.0 41.0 50-51 34.419402592793105 38.0 33.0 40.0 24.0 41.0 52-53 34.19178447154599 38.0 33.0 40.0 23.5 41.0 54-55 33.923271115583944 38.0 33.0 40.0 23.0 41.0 56-57 33.15467351967958 37.5 32.0 40.0 17.5 41.0 58-59 30.160235826910505 35.5 25.5 39.0 7.5 40.5 60-61 29.925143264061894 34.5 25.5 39.0 9.0 40.0 62-63 31.715139827072246 35.5 30.5 39.0 10.5 40.0 64-65 31.7733833231871 36.0 31.0 39.0 6.5 40.0 66-67 26.982138661191446 31.5 18.5 37.0 2.0 39.5 68-69 19.61080787227877 19.5 8.0 32.5 2.0 37.0 70-71 17.7039291755493 15.5 7.5 29.0 2.0 35.0 72-73 19.410535608785217 20.0 7.5 31.0 2.0 35.5 74-75 20.841279368851886 23.5 8.0 32.5 2.0 36.0 76-77 22.62197674079033 27.5 11.0 33.0 2.0 35.5 78-79 24.756357195326594 30.5 13.0 34.5 2.0 37.0 80-81 26.33529473871016 32.0 22.0 35.0 2.0 37.0 82-83 27.45330478909688 33.0 26.0 35.0 2.0 36.5 84-85 27.491691919929373 34.0 26.0 35.0 2.0 36.0 86-87 27.210969388328163 33.5 26.0 35.0 2.0 36.0 88-89 26.768803366003453 33.0 25.5 35.0 2.0 35.5 90-91 24.96919109437794 32.0 13.0 34.5 2.0 35.0 92-93 23.39618381829332 31.0 2.0 34.0 2.0 35.0 94-95 19.698170308452973 27.0 2.0 34.0 2.0 35.0 96-97 17.03321277661589 17.5 2.0 33.0 2.0 35.0 98-99 17.327595207982803 20.0 2.0 33.0 2.0 35.0 100-101 16.576173574716336 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.15085263253254055 1101 2 0.17632348148814714 1101 3 0.09072152063230732 1101 4 0.10114963348429029 1101 5 0.2675840635150877 1101 6 0.15217714827277007 1101 7 0.19894343248464708 1101 8 0.1900267588556872 1101 9 0.24894652970225195 1101 10-11 0.20121068090269745 1101 12-13 0.20321282334684554 1101 14-15 0.29985763057253934 1101 16-17 0.24579604896957363 1101 18-19 0.20342157844882536 1101 20-21 0.22881301846108926 1101 22-23 0.3073733160420815 1101 24-25 0.23839564828450222 1101 26-27 0.21664270585250023 1101 28-29 0.2988422886218771 1101 30-31 0.2113534143106861 1101 32-33 0.09236317069164812 1101 34-35 0.23127594566548737 1101 36-37 0.17517113660081662 1101 38-39 0.11667932219976507 1101 40-41 0.17189363345610786 1101 42-43 0.16314270596045333 1101 44-45 0.24360521844121763 1101 46-47 0.1671445349461962 1101 48-49 -0.18139391730674248 1101 50-51 0.07296673963163158 1101 52-53 -0.021503267586027164 1101 54-55 -0.005548660642212155 1101 56-57 -3.3959482687268903 1101 58-59 -9.85710152070574 1101 60-61 -11.540593946066657 1101 62-63 -3.916461748908631 1101 64-65 -1.4490534102247032 1101 66-67 -2.1686839206127964 1101 68-69 -4.428387430312267 1101 70-71 -4.424908757742951 1101 72-73 -3.6753581513921176 1101 74-75 -6.355763189782827 1101 76-77 -4.809410461072012 1101 78-79 1.1643057196723312 1101 80-81 1.845032564832568 1101 82-83 0.3227138178393396 1101 84-85 0.15745190900682005 1101 86-87 0.08703060367682625 1101 88-89 -0.07837675391229837 1101 90-91 1.12298869696723 1101 92-93 0.6698386438564263 1101 94-95 -8.027303862335902 1101 96-97 -9.095670034114018 1101 98-99 -8.055709131779693 1101 100-101 -8.962264381709453 1102 1 0.2247757927555014 1102 2 0.2422836565925266 1102 3 0.23710838228205589 1102 4 0.19840879594053717 1102 5 0.300771220891221 1102 6 0.31608135010296223 1102 7 0.3081911708220133 1102 8 0.3094094146872379 1102 9 0.11854622538630366 1102 10-11 0.2459116874154219 1102 12-13 0.2656616310848108 1102 14-15 0.37881220405471794 1102 16-17 0.43527337405692634 1102 18-19 0.4176636875546862 1102 20-21 0.3313716345597726 1102 22-23 0.4459026447984016 1102 24-25 0.3556079055910004 1102 26-27 0.2186919301842849 1102 28-29 0.31924940323425943 1102 30-31 0.33181168283882556 1102 32-33 0.31231427153618796 1102 34-35 0.4268798533031415 1102 36-37 0.0161541660783584 1102 38-39 0.11051631069894796 1102 40-41 0.07964057572504402 1102 42-43 0.14336331968473104 1102 44-45 -0.18389158348038848 1102 46-47 -0.049652969172193195 1102 48-49 0.1506410115379353 1102 50-51 0.13143673904876607 1102 52-53 0.007448161502523476 1102 54-55 -0.10729855403960187 1102 56-57 -3.5201560349189833 1102 58-59 -9.953057677121684 1102 60-61 -7.8759243528131435 1102 62-63 -2.208439787236493 1102 64-65 -0.6473660979296625 1102 66-67 -1.8058239642355112 1102 68-69 -5.016092237706655 1102 70-71 -4.79253515469734 1102 72-73 -3.616039165696808 1102 74-75 -4.083760103675527 1102 76-77 -3.8578398748036555 1102 78-79 1.9166713006795781 1102 80-81 2.180558920986531 1102 82-83 0.8289086567390171 1102 84-85 0.4577645077484469 1102 86-87 0.21875768871657186 1102 88-89 0.10149598250775682 1102 90-91 1.4517810657539876 1102 92-93 1.1218099485803776 1102 94-95 -8.431797891055721 1102 96-97 -9.4265308003644 1102 98-99 -8.663172618467405 1102 100-101 -9.603068027365435 1103 1 0.23796352291941858 1103 2 0.3234939213621999 1103 3 0.2504961414053888 1103 4 0.18738854375477842 1103 5 0.23000985693255416 1103 6 0.2064617484591409 1103 7 0.22226007333476616 1103 8 0.2735833324036818 1103 9 0.4114867705100025 1103 10-11 0.40555588217198135 1103 12-13 0.3528735110136978 1103 14-15 0.4644147091935267 1103 16-17 0.5399122172924393 1103 18-19 0.3289310650705346 1103 20-21 0.46574444674303095 1103 22-23 0.4365645391323554 1103 24-25 0.4856591965988031 1103 26-27 0.5380980606240655 1103 28-29 0.436970924111165 1103 30-31 0.4830139837689629 1103 32-33 0.4708691294783094 1103 34-35 0.4908383666998546 1103 36-37 0.45127201149776397 1103 38-39 0.3730740989160495 1103 40-41 0.3048897909099253 1103 42-43 0.34340981895403644 1103 44-45 0.3354097183498723 1103 46-47 0.40934967747963924 1103 48-49 0.29164761995735233 1103 50-51 0.3339921836429838 1103 52-53 0.651149563048719 1103 54-55 0.3815686774849567 1103 56-57 0.7541689419806588 1103 58-59 -8.396363461690491 1103 60-61 -7.199887672875899 1103 62-63 -1.8371666327648732 1103 64-65 0.016015764817630895 1103 66-67 -1.4811853598405413 1103 68-69 -3.3472218937911897 1103 70-71 -3.7748318454122636 1103 72-73 -2.7920612912881246 1103 74-75 -3.558743235561785 1103 76-77 -0.06712894351957388 1103 78-79 3.790215119710094 1103 80-81 3.2376961109976214 1103 82-83 1.7588804213079143 1103 84-85 1.1137673171104012 1103 86-87 0.9413072593260488 1103 88-89 0.8071386277862516 1103 90-91 2.024120083492374 1103 92-93 1.7954427357152305 1103 94-95 -7.145795290679249 1103 96-97 -8.888648636827291 1103 98-99 -7.974234257241974 1103 100-101 -9.260208128245711 1104 1 0.11847072727814023 1104 2 0.16048833073607227 1104 3 0.13523478966274283 1104 4 0.11086589132542457 1104 5 0.1758887303466281 1104 6 0.20503218196059692 1104 7 0.24059754584661164 1104 8 0.18128366317327504 1104 9 0.23823425144607313 1104 10-11 0.24200873382893207 1104 12-13 0.24632667994688973 1104 14-15 0.3687812690073855 1104 16-17 0.3058954636483264 1104 18-19 0.31886064433306416 1104 20-21 0.23924312840555473 1104 22-23 0.2612723350106805 1104 24-25 0.3035637876224371 1104 26-27 0.17634753456547259 1104 28-29 0.39245396806668964 1104 30-31 0.27745836743252283 1104 32-33 0.2710946873556921 1104 34-35 0.15768154106685728 1104 36-37 0.23079857700071926 1104 38-39 0.18727348697405688 1104 40-41 0.15174164478780483 1104 42-43 0.28850881278720664 1104 44-45 0.36572582759501415 1104 46-47 0.23892620487297478 1104 48-49 0.1498769267999478 1104 50-51 0.1197240524000236 1104 52-53 0.09366074977126004 1104 54-55 0.04483355938161537 1104 56-57 0.4550440044330202 1104 58-59 -8.36029024746339 1104 60-61 -7.735173166787476 1104 62-63 -2.277808624822029 1104 64-65 -0.7295483851555673 1104 66-67 -1.7996090804332319 1104 68-69 -3.5663797259557075 1104 70-71 -3.9832808751669404 1104 72-73 -3.1324904894544865 1104 74-75 -3.7995845281524723 1104 76-77 -0.42726500761666486 1104 78-79 3.2465148764649783 1104 80-81 2.591817419926212 1104 82-83 0.9106487804792067 1104 84-85 0.45154361183449865 1104 86-87 0.4892420462951108 1104 88-89 0.3176811336850349 1104 90-91 1.8428793145898261 1104 92-93 1.6499832504321112 1104 94-95 -6.867819184862153 1104 96-97 -8.173071649930433 1104 98-99 -6.1424936870929265 1104 100-101 -5.73036793335787 1105 1 0.17750282145275875 1105 2 0.24912943592583758 1105 3 0.19537884771835579 1105 4 0.11920778238467022 1105 5 0.26133627238758805 1105 6 0.20830033244685353 1105 7 0.19588513948615116 1105 8 0.2042515185531144 1105 9 0.2627659186352105 1105 10-11 0.23441866151163993 1105 12-13 0.1853850418359997 1105 14-15 0.2701040231898659 1105 16-17 0.30451091367660865 1105 18-19 0.2783617018878246 1105 20-21 0.26723716141855647 1105 22-23 0.42408748452766787 1105 24-25 0.34292518309026576 1105 26-27 0.30008594560295876 1105 28-29 0.32659406975048455 1105 30-31 0.33588187217259957 1105 32-33 0.3103299689679275 1105 34-35 0.37051776961398986 1105 36-37 0.384764317271042 1105 38-39 0.39691110781816263 1105 40-41 0.41164446505394636 1105 42-43 0.5332869785152141 1105 44-45 0.41084720317288514 1105 46-47 0.4163087658490241 1105 48-49 0.3753375126168237 1105 50-51 0.3187668482361019 1105 52-53 0.2470614999685381 1105 54-55 0.19069288117733407 1105 56-57 0.8717124727261947 1105 58-59 -7.733149644927423 1105 60-61 -6.92290787368086 1105 62-63 -1.6805316031268376 1105 64-65 -0.09243577989553842 1105 66-67 -1.4022281048885112 1105 68-69 -3.38691106164557 1105 70-71 -3.8258617772053007 1105 72-73 -2.7419582169188423 1105 74-75 -3.6634174164840587 1105 76-77 -0.013333570315072762 1105 78-79 3.85456832712023 1105 80-81 3.2139487801181907 1105 82-83 1.6891090744325865 1105 84-85 1.393555920546806 1105 86-87 1.2777943312980113 1105 88-89 1.3008375472952451 1105 90-91 2.791190830226139 1105 92-93 2.61210819683372 1105 94-95 -5.749487995464364 1105 96-97 -7.393256352680076 1105 98-99 -5.199954762952213 1105 100-101 -4.670493939115868 1106 1 0.07197968215880479 1106 2 0.0640098529320916 1106 3 0.11312380155088775 1106 4 0.10536782455287153 1106 5 0.18666720868539244 1106 6 0.12082314739780742 1106 7 0.19772909622550117 1106 8 0.139147326518966 1106 9 0.1119797927230195 1106 10-11 0.06472241829840186 1106 12-13 0.0467721511677297 1106 14-15 0.08069347962489104 1106 16-17 0.21540678225383658 1106 18-19 0.08690570484335325 1106 20-21 0.12945506147256935 1106 22-23 0.21534227345757984 1106 24-25 0.060641057894585515 1106 26-27 -3.395606733889167E-4 1106 28-29 0.12706783061769045 1106 30-31 0.217323228684009 1106 32-33 0.04273400005723005 1106 34-35 0.10569269656465252 1106 36-37 -0.06633366859136203 1106 38-39 0.025282931177862622 1106 40-41 0.03452843359808355 1106 42-43 0.08267731107323328 1106 44-45 0.14715534634704852 1106 46-47 0.1261521972898052 1106 48-49 0.11066342682936181 1106 50-51 0.09660821383922524 1106 52-53 0.22147917078167012 1106 54-55 0.12682112041331095 1106 56-57 0.7320222870425326 1106 58-59 -8.129591504729703 1106 60-61 -7.509488515490368 1106 62-63 -2.0185221809444513 1106 64-65 -0.37705497631138485 1106 66-67 0.625756040186257 1106 68-69 -2.3408841191682903 1106 70-71 -2.407921369730907 1106 72-73 -1.1453853571768065 1106 74-75 -0.9286778631970982 1106 76-77 2.200998349835217 1106 78-79 4.244322281020228 1106 80-81 3.160388168563859 1106 82-83 1.4060764168078386 1106 84-85 1.069717286784897 1106 86-87 1.0729053385039116 1106 88-89 1.1282785892047613 1106 90-91 2.3031716784751026 1106 92-93 2.0714086513829493 1106 94-95 -5.854128030647825 1106 96-97 -7.432366382888352 1106 98-99 -5.3640327286613285 1106 100-101 -4.515460958230458 1107 1 0.002360160250283627 1107 2 -0.039051846175361504 1107 3 0.041430530451005865 1107 4 0.08351165472078037 1107 5 0.09781743856496661 1107 6 0.10107009205243855 1107 7 0.0842152746976268 1107 8 0.06924743595712357 1107 9 0.11641412711577459 1107 10-11 -0.03161899800726786 1107 12-13 0.08038291662484909 1107 14-15 0.09571251725075314 1107 16-17 0.1730552094014186 1107 18-19 0.1143033506206308 1107 20-21 0.14398904734471785 1107 22-23 0.18792706094111367 1107 24-25 0.201634503315951 1107 26-27 0.16052387689450853 1107 28-29 0.18309559061023606 1107 30-31 0.17457242764944425 1107 32-33 0.10917193204826248 1107 34-35 0.11026835144132718 1107 36-37 0.029056084913200664 1107 38-39 0.13132083792871185 1107 40-41 0.020911763619082535 1107 42-43 -0.02371726166084187 1107 44-45 -0.11727686605352972 1107 46-47 -0.22887195129579396 1107 48-49 -0.2806205689623127 1107 50-51 -0.11402109667587723 1107 52-53 -0.02648426732808673 1107 54-55 -0.09791008662792677 1107 56-57 0.4296603384524218 1107 58-59 -8.914007587193048 1107 60-61 -7.882489643432336 1107 62-63 -2.352261472006788 1107 64-65 -0.4542814206687851 1107 66-67 0.2993504694498945 1107 68-69 -2.749824872762172 1107 70-71 -2.8975229962886004 1107 72-73 -1.4253409736303908 1107 74-75 -1.1603617408983347 1107 76-77 1.871015194677561 1107 78-79 3.889174378689898 1107 80-81 2.941158584566967 1107 82-83 1.3590242991767596 1107 84-85 1.060466082589297 1107 86-87 1.0525495080332554 1107 88-89 0.7814576708366126 1107 90-91 2.0789756889004813 1107 92-93 1.5930732476215006 1107 94-95 -6.892247155513081 1107 96-97 -8.3443197885547 1107 98-99 -6.0969831595183805 1107 100-101 -5.16288566552576 1108 1 -0.042112870422648996 1108 2 -0.11294718977988794 1108 3 -0.08131304252412619 1108 4 -0.04247456160670282 1108 5 -0.06778477322887255 1108 6 -0.12575170680614178 1108 7 -0.09665889581979314 1108 8 -0.04295647670589631 1108 9 -0.07172899886017348 1108 10-11 -0.0920742513769639 1108 12-13 -0.05680437302083874 1108 14-15 -0.1967850008463472 1108 16-17 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2312 94-95 5.566810325511959 2312 96-97 0.7062463687583964 2312 98-99 -0.5170425658301898 2312 100-101 0.12833870701295425 2313 1 -0.2806807541518239 2313 2 -0.39617164581540365 2313 3 -0.3074739783624807 2313 4 -0.2846759086467543 2313 5 -0.38013601645569395 2313 6 -0.3265670205246849 2313 7 -0.40571427975996954 2313 8 -0.44585733928418136 2313 9 -0.5197084387750976 2313 10-11 -0.5309067322601351 2313 12-13 -0.4485305423967674 2313 14-15 -0.5261198365175872 2313 16-17 -0.4998038852246225 2313 18-19 -0.5166120354886203 2313 20-21 -0.5614414492229685 2313 22-23 -0.3874679692901921 2313 24-25 -0.4999210337647355 2313 26-27 -0.5524113497087839 2313 28-29 -0.4783493094273652 2313 30-31 -0.5297763959957322 2313 32-33 -0.5601069169471842 2313 34-35 -0.617861120009259 2313 36-37 -0.6875634708369134 2313 38-39 -0.6625652216349849 2313 40-41 -0.46639370087918053 2313 42-43 -0.3394787210773629 2313 44-45 -0.4856704855553673 2313 46-47 -0.46854089102088636 2313 48-49 -0.6107631714983768 2313 50-51 -0.6386741949222667 2313 52-53 -0.5539352798772157 2313 54-55 -0.7692678455425792 2313 56-57 -0.01756875050342188 2313 58-59 2.938391269934222 2313 60-61 2.928051402494958 2313 62-63 0.2191013368257302 2313 64-65 -0.43449840800793993 2313 66-67 -2.1193219022708263 2313 68-69 -5.067447494835413 2313 70-71 -3.5664727887386007 2313 72-73 -2.815386481346863 2313 74-75 -3.4856940936924197 2313 76-77 0.42069583202590977 2313 78-79 2.8599106729529993 2313 80-81 2.1054046464429135 2313 82-83 0.6573070348228143 2313 84-85 0.30533197235884657 2313 86-87 0.2073189362526513 2313 88-89 0.2669506857122741 2313 90-91 1.9882690965980494 2313 92-93 3.4029295506659345 2313 94-95 5.579598496913576 2313 96-97 1.6706113247199923 2313 98-99 3.173526489234664 2313 100-101 4.2745514540660405 2314 1 -0.41491244424943474 2314 2 -0.3851914049867986 2314 3 -0.578945523577044 2314 4 -0.35084019129148913 2314 5 -0.39457170048435586 2314 6 -0.3448615751320858 2314 7 -0.38445321839515856 2314 8 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2314 72-73 -2.747146959496007 2314 74-75 -3.8227697275557624 2314 76-77 0.5996684954359246 2314 78-79 3.303886888277976 2314 80-81 2.3994461994629184 2314 82-83 0.8308268510002499 2314 84-85 0.49523554444517615 2314 86-87 0.5391598806235258 2314 88-89 0.5193241103722102 2314 90-91 2.228801708439523 2314 92-93 3.702338441854554 2314 94-95 6.303855883581178 2314 96-97 3.7986743032418673 2314 98-99 5.853659006358402 2314 100-101 6.3287389864060035 2315 1 -0.5092831376837843 2315 2 -0.40773111250565464 2315 3 -0.4503156437393585 2315 4 -0.33868457603570334 2315 5 -0.5154534153545782 2315 6 -0.6107799906531426 2315 7 -0.5958871853252319 2315 8 -0.5870970854535074 2315 9 -0.7689964746828508 2315 10-11 -0.6297254688555327 2315 12-13 -0.7598114144422254 2315 14-15 -0.8310930825874081 2315 16-17 -0.7216066800153484 2315 18-19 -0.6509858608584196 2315 20-21 -0.7749941942646501 2315 22-23 -0.8946777112584883 2315 24-25 -0.6317438387263721 2315 26-27 -0.6308810691241789 2315 28-29 -0.7746910964130151 2315 30-31 -0.8020070325623934 2315 32-33 -0.8226785002934136 2315 34-35 -0.8219129032360897 2315 36-37 -0.7034223604382603 2315 38-39 -0.7787038114154328 2315 40-41 -0.8920514937904969 2315 42-43 -0.7820661135641558 2315 44-45 -0.9422583644772544 2315 46-47 -0.7293972378933304 2315 48-49 -0.6629184106254442 2315 50-51 -0.6476472641066096 2315 52-53 -0.6192585381079141 2315 54-55 -0.5354379430507024 2315 56-57 0.09288794571786241 2315 58-59 3.1187370810647366 2315 60-61 3.1527556720684444 2315 62-63 0.5491442315567525 2315 64-65 -0.15281711143730803 2315 66-67 -2.2030313015234846 2315 68-69 -4.966786385321184 2315 70-71 -3.5382418647330276 2315 72-73 -2.775944886724183 2315 74-75 -0.569825807049483 2315 76-77 1.857520074182144 2315 78-79 3.3481024610441814 2315 80-81 2.3222264564236745 2315 82-83 0.5819593442839732 2315 84-85 0.3040633717441672 2315 86-87 0.23846183761688167 2315 88-89 0.3139010693326796 2315 90-91 2.174954041431885 2315 92-93 3.560968996129926 2315 94-95 6.332006350629278 2315 96-97 4.214027315168174 2315 98-99 6.317940224392867 2315 100-101 6.476237946647904 2316 1 -0.43427786525601775 2316 2 -0.3982840619302479 2316 3 -0.47652730463960324 2316 4 -0.3322197246041725 2316 5 -0.5780271580943079 2316 6 -0.45399907855163946 2316 7 -0.4666152363509326 2316 8 -0.4749408619937654 2316 9 -0.7060157575186778 2316 10-11 -0.586966364655261 2316 12-13 -0.6107618764209022 2316 14-15 -0.7152894790967821 2316 16-17 -0.78423571199761 2316 18-19 -0.7587366146730901 2316 20-21 -0.6715617012898036 2316 22-23 -0.867978707034851 2316 24-25 -0.6223355476199615 2316 26-27 -0.7222017980301914 2316 28-29 -0.5488562003510964 2316 30-31 -0.6045965070865904 2316 32-33 -0.44453941079893156 2316 34-35 -0.5145406242363109 2316 36-37 -0.5547113977784406 2316 38-39 -0.6793667123336888 2316 40-41 -0.8687884024948431 2316 42-43 -0.849287002637972 2316 44-45 -0.6589619856376459 2316 46-47 -0.6565892853622799 2316 48-49 -0.7883189726377466 2316 50-51 -0.5573656309649877 2316 52-53 -0.6162632291436481 2316 54-55 -0.596677334071039 2316 56-57 0.3970557286192289 2316 58-59 3.1646043526831136 2316 60-61 3.29939414312139 2316 62-63 0.5885973810486007 2316 64-65 0.05110158487655525 2316 66-67 -2.0124464325361906 2316 68-69 -3.011888727419098 2316 70-71 -2.931273710196745 2316 72-73 -0.728573405713469 2316 74-75 5.504807260477492 2316 76-77 5.784857469659826 2316 78-79 4.785861593744066 2316 80-81 2.9841008187449134 2316 82-83 1.3859760792547036 2316 84-85 0.9800171111779363 2316 86-87 0.7986131135444019 2316 88-89 0.6495842904725464 2316 90-91 2.2602927022922366 2316 92-93 3.767222380458559 2316 94-95 6.394071660334532 2316 96-97 4.035995384205627 2316 98-99 6.090971043870642 2316 100-101 6.028951394473667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 11.0 4 41.0 5 137.0 6 270.0 7 693.0 8 1379.0 9 2479.0 10 4074.0 11 6777.0 12 9733.0 13 13118.0 14 16175.0 15 21236.0 16 27884.0 17 36206.0 18 36009.0 19 31653.0 20 32255.0 21 32444.0 22 33139.0 23 33527.0 24 35762.0 25 37984.0 26 47721.0 27 66475.0 28 94158.0 29 124804.0 30 151728.0 31 169416.0 32 178342.0 33 179275.0 34 183713.0 35 195208.0 36 197378.0 37 155285.0 38 64880.0 39 4408.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50145152621465 20.795697011204165 14.738967951379713 21.96388351120147 2 15.06797425268763 22.514833911861512 40.01379480785001 22.40339702760085 3 18.217004982066985 27.95191624700162 31.696126711364048 22.134952059567354 4 11.95200794326496 18.305227144580453 39.19102698598472 30.55173792616987 5 11.926489192681288 39.71845853530463 34.74247929485156 13.612572977162522 6 28.434732015895513 38.82463939688694 18.577221070319013 14.163407516898532 7 25.611054077550165 35.347708785889026 22.293527901392093 16.747709235168713 8 25.37026295914741 35.02403423873756 20.86959959708561 18.736103205029423 9 26.73456780416797 16.43119169191993 21.52705674627154 35.307183757640566 10-11 23.70395163953392 27.588311539524256 28.53018148653172 20.17755533441011 12-13 25.55527600374699 25.715578997971498 28.67862349687863 20.050521501402876 14-15 22.432001365813335 26.36004007583858 28.110909433996174 23.097049124351912 16-17 20.379443742195168 29.695058079522074 28.66524140140252 21.26025677688024 18-19 20.192494749020003 28.03075333310869 31.86087991823073 19.915871999640576 20-21 21.567851342335402 26.95280541471885 31.456003162929036 20.023340080016713 22-23 20.966760041962722 27.265885968321292 31.094040978800734 20.67331301091525 24-25 20.865929562268352 27.32431283124601 31.49102910595672 20.318728500528916 26-27 20.80043382534801 27.308375646205903 31.64231603251084 20.248874495935247 28-29 20.305298102804482 27.465439879160325 31.852482681188754 20.376779336846443 30-31 20.604279329066763 27.46749751536163 31.64283570474768 20.28538745082393 32-33 20.339005135331796 27.611254102435705 31.38406840758885 20.665672354643647 34-35 20.89911099349745 27.38224943311065 31.331514914345455 20.387124659046442 36-37 20.995964463535373 27.071034255741665 31.31419354026363 20.61880774045933 38-39 20.768560601376638 27.54465976961618 31.31160496380791 20.37517466519927 40-41 20.665610206769646 27.41054754092021 31.50288315520562 20.420959097104525 42-43 20.456708624637344 27.663074969639478 30.966295489913513 20.913920915809666 44-45 20.760956160069362 27.70363328144355 30.592701507247067 20.942709051240012 46-47 20.878195051875643 27.84695454464714 30.040564699470234 21.23428570400698 48-49 21.0232775854766 27.93123651606022 29.865437138198747 21.18004876026443 50-51 20.900393107600333 28.20271636767318 29.704555560901102 21.19233496382538 52-53 21.02887528736033 28.481710205020228 29.505496345772446 20.983918161847 54-55 20.787106910110907 28.09403028181439 29.618235167855833 21.50062764021887 56-57 21.031557722071298 28.61523452208209 29.06581184957227 21.28739590627434 58-59 20.33671671232381 27.85379007064604 30.51594455993829 21.29354865709186 60-61 20.492697945634465 28.168244633414314 30.328852191743188 21.010205229208037 62-63 21.30103447725864 28.01528588472865 29.50289873337247 21.180780904640244 64-65 20.89050374057219 28.143228578142264 29.571255080919556 21.39501260036599 66-67 21.084989012096912 28.085911369243956 29.381139681021562 21.447959937637577 68-69 20.753745465851622 27.720069331391773 30.339451995002285 21.186733207754315 70-71 21.572727874726613 27.93372672875398 29.149391236528384 21.344154159991024 72-73 20.813891371382105 27.82036130262136 29.723173294085036 21.642574031911497 74-75 20.70470020041995 28.136981689214856 29.539398103176463 21.61892000718873 76-77 19.5165466831512 28.424576015002618 30.16567952544815 21.893197776398033 78-79 20.675686755756253 28.24028303031083 29.233229182407328 21.850801031525588 80-81 20.46626279187774 28.687784473068344 28.907520044297684 21.93843269075623 82-83 20.723251261073354 28.702602343220867 28.559551528507388 22.01459486719839 84-85 20.609613367149574 28.317110373783276 28.47905542164344 22.594220837423705 86-87 20.594027110776246 28.39622654225057 28.69576508127347 22.313981265699713 88-89 20.886802332751593 28.043405838887747 28.618012488636026 22.451779339724638 90-91 20.50703431800639 28.27497225148884 28.815699177967964 22.402294252536805 92-93 20.35573659992354 28.50192403343757 28.881381501184357 22.260957865454536 94-95 19.602840564884445 28.323552510819212 29.248763000286576 22.824843924009766 96-97 19.475081414853378 28.453366677155113 28.80560494056065 23.26594696743086 98-99 19.89696990246273 28.097243413691388 28.675504922071905 23.33028176177398 100-101 19.155363341808567 28.257114950567153 29.354648819882286 23.232872887741994 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1778.0 1 1349.5 2 962.5 3 1045.5 4 1068.5 5 1201.0 6 1570.5 7 2390.0 8 3387.0 9 4016.0 10 4206.0 11 4209.5 12 4295.0 13 4455.0 14 4646.5 15 4946.5 16 5268.0 17 5496.5 18 5831.0 19 6267.5 20 6909.5 21 7761.0 22 8665.0 23 9847.0 24 11373.0 25 13709.0 26 17184.0 27 21167.5 28 24917.5 29 28820.0 30 33346.5 31 38742.0 32 45328.0 33 52002.0 34 58777.5 35 65056.5 36 70659.5 37 75108.0 38 76717.0 39 76830.5 40 76513.5 41 76615.0 42 77417.0 43 78663.0 44 80135.5 45 80774.0 46 81361.5 47 83249.0 48 86672.0 49 83629.0 50 74674.5 51 68091.5 52 62804.5 53 58083.5 54 53368.0 55 48101.5 56 43063.0 57 38310.5 58 33825.5 59 29398.5 60 24814.5 61 20116.0 62 15580.0 63 11739.0 64 8613.0 65 6210.0 66 4316.0 67 2978.5 68 1968.0 69 1245.5 70 878.5 71 652.5 72 484.5 73 377.5 74 264.0 75 165.0 76 100.0 77 52.0 78 24.0 79 14.0 80 9.0 81 3.5 82 2.0 83 1.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02430603135522973 2 0.013927670462331268 3 8.985593846665335E-5 4 0.0 5 0.06554990711142361 6 0.0 7 0.0 8 8.985593846665335E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 2.2463984616663335E-4 16-17 2.2463984616663337E-5 18-19 4.492796923332667E-4 20-21 0.0 22-23 0.0 24-25 1.5724789231664333E-4 26-27 2.0217586154997E-4 28-29 0.0011681272000664935 30-31 0.004897148646432608 32-33 0.005638460138782497 34-35 0.0018869747077997201 36-37 0.09169798520521973 38-39 0.0848015419279041 40-41 0.09012550628205329 42-43 0.09396684765150272 44-45 0.09711180549783559 46-47 0.09225958482063631 48-49 0.08280224729702106 50-51 0.07691668332745526 52-53 0.06485352358830704 54-55 0.05312732361840879 56-57 0.06862747300390648 58-59 0.6950356840395635 60-61 0.32642416046473494 62-63 0.003212349800182857 64-65 0.002515966277066293 66-67 1.0700943711993747 68-69 2.3178114687627063 70-71 2.3008960883463585 72-73 1.7684996529314374 74-75 1.253490341609814 76-77 0.7205098425948597 78-79 0.5467958495542022 80-81 0.037649638217527745 82-83 1.1231992308331667E-4 84-85 0.0013478390769998 86-87 0.0012579831385331466 88-89 6.739195384999E-5 90-91 0.3233690585568687 92-93 0.052183836264508926 94-95 0.9187320428522971 96-97 1.620731562123026 98-99 0.6204552551122413 100-101 0.5515806782775515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2225785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.59905382154922 #Duplication Level Percentage of deduplicated Percentage of total 1 70.51823425205504 30.74528290555963 2 14.553799657904204 12.690637891860199 3 5.845271183375326 7.645448787765946 4 2.8604803296716144 4.988569433953423 5 1.6147638768433443 3.5201088587793223 6 1.03483466343869 2.707068731260093 7 0.6674756852994926 2.0370915829563616 8 0.5063642370077105 1.7661601298085496 9 0.33894334219745437 1.3299848117030328 >10 1.80217535782416 14.27002652136383 >50 0.14975869227152092 4.545582395354067 >100 0.09850563736276405 8.156263250787767 >500 0.006502904826011397 1.8401380353190258 >1k 0.002580517788099751 2.0392324291230106 >5k 1.0322071152399004E-4 0.4180352224333777 >10k+ 2.0644142304798008E-4 1.3003690119724889 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16563 0.7441419544115896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12332 0.5540517165853844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9289 0.41733590620837147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3906 0.175488647825374 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3803 0.1708610669943413 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3128 0.14053468776184583 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2676 0.12022724566838217 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2551 0.11461124951421633 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2543 0.11425182576034973 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2280 0.10243576985198481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.4927969233326666E-5 0.0 5 0.0 0.0 0.0 4.4927969233326666E-5 0.0 6 0.0 0.0 0.0 4.4927969233326666E-5 0.0 7 0.0 0.0 0.0 4.4927969233326666E-5 0.0 8 0.0 0.0 0.0 4.4927969233326666E-5 0.0 9 0.0 0.0 0.0 8.985593846665333E-5 0.0 10-11 0.0 0.0 0.0 1.5724789231664333E-4 0.0 12-13 0.0 0.0 0.0 2.2463984616663335E-4 0.0 14-15 0.0 0.0 0.0 2.9203180001662337E-4 0.0 16-17 0.0 0.0 0.0 4.942076615665934E-4 0.0 18-19 0.0 0.0 0.0 5.3913563079992E-4 0.0 20-21 0.0 0.0 0.0 6.739195384999001E-4 0.0 22-23 0.0 0.0 0.0 0.0010558072769831766 0.0 24-25 0.0 0.0 0.0 0.001931902677033047 0.0 26-27 0.0 0.0 0.0 0.00303263792324955 0.0 28-29 0.0 0.0 0.0 0.006042811861882437 0.0 30-31 0.0 0.0 0.0 0.01637624478554757 0.0 32-33 0.0 0.0 0.0 0.036683686879011225 0.0 34-35 0.0 0.0 0.0 0.06579701094220691 0.0 36-37 0.0 0.0 0.0 0.11299384262181658 0.0 38-39 0.0 0.0 0.0 0.17937491716405674 0.0 40-41 0.0 2.2463984616663333E-5 0.0 0.25678580815307855 0.0 42-43 0.0 4.4927969233326666E-5 0.0 0.33123145317270086 0.0 44-45 0.0 4.4927969233326666E-5 0.0 0.4239852456549038 0.0 46-47 0.0 4.4927969233326666E-5 0.0 0.5301500369532547 0.0 48-49 0.0 4.4927969233326666E-5 0.0 0.640762697205705 0.0 50-51 0.0 4.4927969233326666E-5 0.0 0.7515775333197052 0.0 52-53 0.0 4.4927969233326666E-5 0.0 0.859157555648906 0.0 54-55 0.0 4.4927969233326666E-5 0.0 0.9792724813942048 0.0 56-57 0.0 4.4927969233326666E-5 0.0 1.0924460358929546 0.0 58-59 0.0 4.4927969233326666E-5 0.0 1.179898328005625 0.0 60-61 0.0 4.4927969233326666E-5 0.0 1.255961379917647 0.0 62-63 4.4927969233326666E-5 4.4927969233326666E-5 0.0 1.3213989671059871 0.0 64-65 4.4927969233326666E-5 4.4927969233326666E-5 0.0 1.3949235887563263 0.0 66-67 8.985593846665333E-5 6.739195384998999E-5 0.0 1.4572386820829506 0.0 68-69 8.985593846665333E-5 8.985593846665333E-5 0.0 1.5349640688566057 0.0 70-71 8.985593846665333E-5 8.985593846665333E-5 0.0 1.653776083494138 0.0 72-73 8.985593846665333E-5 1.1231992308331667E-4 0.0 1.8136073340416976 0.0 74-75 8.985593846665333E-5 1.3478390769998E-4 0.0 2.026161556484566 0.0 76-77 8.985593846665333E-5 1.3478390769998E-4 0.0 2.2979083783923424 0.0 78-79 8.985593846665333E-5 1.3478390769998E-4 0.0 2.631610869872876 0.0 80-81 8.985593846665333E-5 1.3478390769998E-4 0.0 3.027269030926168 0.0 82-83 8.985593846665333E-5 1.3478390769998E-4 0.0 3.4255554781796085 0.0 84-85 8.985593846665333E-5 1.3478390769998E-4 0.0 3.7862596791693717 0.0 86-87 8.985593846665333E-5 1.3478390769998E-4 0.0 4.123736120065505 0.0 88-89 8.985593846665333E-5 1.3478390769998E-4 0.0 4.4379848008680085 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13105 0.0 27.01455 1 GGTATCA 15035 0.0 26.52936 1 CATTCCG 465 0.0 26.364851 9 TACATGG 13315 0.0 26.29896 2 ACATGGG 13105 0.0 25.492561 3 GTATCAA 20945 0.0 25.444098 1 CATGGGA 9485 0.0 23.43154 4 GAGTACT 11845 0.0 22.493011 12-13 CATGGGG 3695 0.0 21.837297 4 GTCGGGA 680 0.0 21.51437 2 ATCAACG 24795 0.0 21.276241 3 TCAACGC 24880 0.0 21.203554 4 AGTACTT 12430 0.0 21.13091 12-13 ATGGGAT 3975 0.0 21.130032 5 CAACGCA 25230 0.0 20.872005 5 AACGCAG 25225 0.0 20.861137 6 TATCAAC 25630 0.0 20.659512 2 GTACTTT 13225 0.0 20.235054 14-15 CGGGAGT 730 0.0 20.038078 4 CGCAGAG 27310 0.0 19.009493 8 >>END_MODULE